MD01G1056200.v1.1

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009833
Physical Location & Seq
Reverse (-)
16139887 .. 16144271
4385 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1056200.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGGATCGTAGGGACCCTATGGCATTGTCGGGGTCAGCCTCTTACTTTACATCTAGAGGAATAACTGGGTCCGGAACACTGTCTGGATTACATGGATCGCCAGGCATTCATCCCTTATCTAATCCAAATATAGCATTTCAATCTAATATTGGAGGAACTAACATTGGGTCAACATTGCCGGTAGAGCCTTCTTCAGCTATTTCATACCATGGTGTCAATGTGGGTGCACCAACTGTGGCACCACCAGGCGAATCTTTGAAAAGGAAAAGAGGAAGGCCTCGAAAATATGGACCTGATGGGACTGTTTCATTGGCATTGTCTCCTGCAGCATCTGCTAATCCTGGTACGGTATCATCAAGCCCAAAACGAGGTAGAGGGCGGCCACCAGGGTCTGGGAAGAAGCAACAATTAGCATCTCTTGGCGGATTGCTGTCTGGTTCGGCTGGGATAGGTTTTACTCCACATATTATCAGCATTGCAGTGGGAGAAGACGTTGCAACAAAAATAATGGCGTTTTCACAGCAAGGGCCAAGAGCTGTGTGCATCTTGTCAGCTAATGGTGCCGTCTCTACTGTGACACTTCGTCAGCCCTCAACTTCTGGGGGTACAGTCACATATGAGGGACGGTTTGAGATTATATGTCTCTCAGGTTCTTACTTGCTCAATGAAATTGGTAGCTCCCGAAACAGAAGTGGCGGTCTGAGCGTCTCCCTTGCTAGTCCTGATGGTCGTGTCATTGGTGGTGGAGTAGGAGGGATGCTTATTGCAGCAACCCCTGTTCAGGTGATATTAGGTAGCTTCACATGGGGTGGTTTGAAGACAAAGAACAAGAAAAAGGAAGCTGTAGAAGGTGTCACAGACTTGGAACATGAGACGGTTGACAATTCAGTTGCACTAAACAGCATTCAACCAGATCAAAGTCTTAGCCAAAGTGCTTCATTGGCCGCGTGGCAGGCATCACAGCCACTGGATATGCATAACAGTCATGTTGACATTGATTTGATGCGAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

338

Amino Acids

34.38

Weight (kDa)

9.65

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 153 - 268 1.2e-25 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 238, 560
AccB7I CCANNNNNTGG 1 cut(s) 392
AccII CGCG 1 cut(s) 947
AccIII TCCGGA 1 cut(s) 71
AciI CCGC 4 cut(s) 379, 423, 696, 945
AclWI GGATC 2 cut(s) 12, 103
AcoI YGGCCR 2 cut(s) 380, 942
AcuI CTGAAG 1 cut(s) 177
AfaI GTAC 2 cut(s) 346, 607
AfiI CCNNNNNNNGG 3 cut(s) 368, 392, 781
AgsI TTSAA 4 cut(s) 140, 259, 817, 908
AhdI GACNNNNNGTC 1 cut(s) 582
AjnI CCWGG 4 cut(s) 100, 244, 340, 385
AluBI AGCT 6 cut(s) 197, 536, 554, 678, 798, 842
AluI AGCT 6 cut(s) 197, 536, 554, 678, 798, 842
Alw21I GWGCWC 1 cut(s) 229
Alw26I GTCTC 5 cut(s) 324, 571, 647, 712, 866
Alw44I GTGCAC 1 cut(s) 225
AlwI GGATC 2 cut(s) 12, 103
AlwNI CAGNNNCTG 3 cut(s) 332, 392, 967
Aor13HI TCCGGA 1 cut(s) 71
AoxI GGCC 4 cut(s) 275, 380, 527, 942
ApaLI GTGCAC 1 cut(s) 225
ApeKI GCWGC 2 cut(s) 326, 767
AspS9I GGNCC 4 cut(s) 13, 69, 290, 527
AsuHPI GGTGA 1 cut(s) 796
AvaII GGWCC 3 cut(s) 13, 69, 290
BaeGI GKGCMC 1 cut(s) 229
BanI GGYRCC 2 cut(s) 238, 560
BbsI GAAGAC 2 cut(s) 495, 824
Bbv12I GWGCWC 1 cut(s) 229
BbvI GCAGC 2 cut(s) 338, 779
BccI CCATC 2 cut(s) 290, 719
BceAI ACGGC 1 cut(s) 548
BciT130I CCWGG 4 cut(s) 102, 246, 342, 387
BcoDI GTCTC 5 cut(s) 324, 571, 647, 712, 866
BfaI CTAG 2 cut(s) 54, 717
BfmI CTRYAG 2 cut(s) 324, 843
BisI GCNGC 4 cut(s) 327, 380, 768, 945
BlsI GCNGC 4 cut(s) 328, 381, 769, 946
Bme1390I CCNGG 4 cut(s) 102, 246, 342, 387
Bme18I GGWCC 3 cut(s) 13, 69, 290
BmeRI GACNNNNNGTC 1 cut(s) 582
BmgT120I GGNCC 4 cut(s) 13, 69, 290, 527
BmiI GGNNCC 5 cut(s) 14, 15, 70, 240, 562
BmrFI CCNGG 4 cut(s) 102, 246, 342, 387
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 6 cut(s) 338, 422, 552, 747, 965, 993
BmuI ACTGGG 1 cut(s) 75
BpiI GAAGAC 2 cut(s) 495, 824
BsaJI CCNNGG 2 cut(s) 208, 386
BsaWI WCCGGW 1 cut(s) 71
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bsc4I CCNNNNNNNGG 3 cut(s) 368, 392, 781
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 2 cut(s) 70, 972
Bse3DI GCAATG 2 cut(s) 173, 474
BseAI TCCGGA 1 cut(s) 71
BseBI CCWGG 4 cut(s) 102, 246, 342, 387
BseDI CCNNGG 2 cut(s) 208, 386
BseGI GGATG 2 cut(s) 109, 762
BseLI CCNNNNNNNGG 3 cut(s) 368, 392, 781
BseMI GCAATG 2 cut(s) 173, 474
BseMII CTCAG 2 cut(s) 660, 692
BseNI ACTGG 2 cut(s) 70, 972
BseSI GKGCMC 1 cut(s) 229
BseXI GCAGC 2 cut(s) 338, 779
BseYI CCCAGC 1 cut(s) 443
Bsh1236I CGCG 1 cut(s) 947
BshFI GGCC 4 cut(s) 277, 382, 529, 944
BshNI GGYRCC 2 cut(s) 238, 560
BsiHKAI GWGCWC 1 cut(s) 229
BsiSI CCGG 2 cut(s) 72, 179
BslFI GGGAC 3 cut(s) 26, 313, 636
BslI CCNNNNNNNGG 3 cut(s) 368, 392, 781
BsmAI GTCTC 5 cut(s) 324, 571, 647, 712, 866
BsmBI CGTCTC 3 cut(s) 571, 712, 866
BsmFI GGGAC 3 cut(s) 26, 313, 636
BsmI GAATGC 2 cut(s) 105, 903
BsnI GGCC 4 cut(s) 277, 382, 529, 944
Bsp1286I GDGCHC 1 cut(s) 229
Bsp13I TCCGGA 1 cut(s) 71
Bsp143I GATC 3 cut(s) 4, 95, 913
Bsp19I CCATGG 1 cut(s) 208
BspACI CCGC 4 cut(s) 379, 423, 696, 945
BspANI GGCC 4 cut(s) 277, 382, 529, 944
BspCNI CTCAG 2 cut(s) 659, 693
BspEI TCCGGA 1 cut(s) 71
BspFNI CGCG 1 cut(s) 947
BspLI GGNNCC 5 cut(s) 14, 15, 70, 240, 562
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 2 cut(s) 12, 103
BspT107I GGYRCC 2 cut(s) 238, 560
BsrDI GCAATG 2 cut(s) 173, 474
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 2 cut(s) 70, 972
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 208, 386
BssMI GATC 3 cut(s) 4, 95, 913
BssT1I CCWWGG 1 cut(s) 208
Bst2UI CCWGG 4 cut(s) 102, 246, 342, 387
Bst4CI ACNGT 9 cut(s) 81, 235, 304, 349, 574, 610, 627, 877, 983
BstAPI GCANNNNNTGC 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 954
BstDEI CTNAG 3 cut(s) 646, 701, 923
BstDSI CCRYGG 1 cut(s) 208
BstF5I GGATG 2 cut(s) 109, 762
BstFNI CGCG 1 cut(s) 947
BstKTI GATC 3 cut(s) 7, 98, 916
BstMAI GTCTC 5 cut(s) 324, 571, 647, 712, 866
BstMBI GATC 3 cut(s) 4, 95, 913
BstMWI GCNNNNNNNGC 6 cut(s) 184, 332, 560, 702, 941, 953
BstNI CCWGG 4 cut(s) 102, 246, 342, 387
BstSCI CCNGG 4 cut(s) 100, 244, 340, 385
BstSFI CTRYAG 2 cut(s) 324, 843
BstSLI GKGCMC 1 cut(s) 229
BstUI CGCG 1 cut(s) 947
BstV1I GCAGC 2 cut(s) 338, 779
BstV2I GAAGAC 2 cut(s) 495, 824
BsuRI GGCC 4 cut(s) 277, 382, 529, 944
BtgI CCRYGG 1 cut(s) 208
BtsCI GGATG 2 cut(s) 109, 762
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 3 cut(s) 77, 486, 965
Cac8I GCNNGC 1 cut(s) 954
CaiI CAGNNNCTG 3 cut(s) 332, 392, 967
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 4 cut(s) 13, 69, 290, 527
CseI GACGC 1 cut(s) 694
Csp6I GTAC 2 cut(s) 345, 606
CviAII CATG 5 cut(s) 92, 209, 804, 869, 986
CviQI GTAC 2 cut(s) 345, 606
DdeI CTNAG 3 cut(s) 646, 701, 923
DpnI GATC 3 cut(s) 6, 97, 915
DpnII GATC 3 cut(s) 4, 95, 913
DriI GACNNNNNGTC 1 cut(s) 582
EaeI YGGCCR 2 cut(s) 380, 942
Eam1105I GACNNNNNGTC 1 cut(s) 582
EciI GGCGGA 1 cut(s) 438
Eco130I CCWWGG 1 cut(s) 208
Eco147I AGGCCT 1 cut(s) 277
Eco47I GGWCC 3 cut(s) 13, 69, 290
Eco57I CTGAAG 1 cut(s) 177
EcoO109I RGGNCCY 1 cut(s) 13
EcoRII CCWGG 4 cut(s) 100, 244, 340, 385
EcoT14I CCWWGG 1 cut(s) 208
EcoT22I ATGCAT 1 cut(s) 978
ErhI CCWWGG 1 cut(s) 208
Esp3I CGTCTC 3 cut(s) 571, 712, 866
FaeI CATG 5 cut(s) 95, 212, 807, 872, 989
FaqI GGGAC 3 cut(s) 26, 313, 636
FatI CATG 5 cut(s) 91, 208, 803, 868, 985
FauNDI CATATG 1 cut(s) 616
Fnu4HI GCNGC 4 cut(s) 327, 380, 768, 945
FokI GGATG 2 cut(s) 96, 769
Fsp4HI GCNGC 4 cut(s) 327, 380, 768, 945
FspBI CTAG 2 cut(s) 54, 717
GluI GCNGC 4 cut(s) 327, 380, 768, 945
GsaI CCCAGC 1 cut(s) 447
HaeIII GGCC 4 cut(s) 277, 382, 529, 944
HapII CCGG 2 cut(s) 72, 179
HgaI GACGC 1 cut(s) 694
Hin1II CATG 5 cut(s) 95, 212, 807, 872, 989
HincII GTYRAC 3 cut(s) 171, 880, 991
HindII GTYRAC 3 cut(s) 171, 880, 991
HinfI GANTC 1 cut(s) 251
HpaII CCGG 2 cut(s) 72, 179
HphI GGTGA 1 cut(s) 796
Hpy166II GTNNAC 4 cut(s) 171, 227, 880, 991
Hpy188I TCNGA 1 cut(s) 702
Hpy188III TCNNGA 5 cut(s) 54, 72, 84, 681, 722
Hpy8I GTNNAC 4 cut(s) 171, 227, 880, 991
HpyAV CCTTC 3 cut(s) 198, 267, 842
HpyCH4III ACNGT 9 cut(s) 81, 235, 304, 349, 574, 610, 627, 877, 983
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 8 cut(s) 227, 326, 479, 497, 543, 767, 893, 976
HpyF10VI GCNNNNNNNGC 6 cut(s) 184, 332, 560, 702, 941, 953
HpyF3I CTNAG 3 cut(s) 646, 701, 923
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 5 cut(s) 95, 212, 807, 872, 989
KflI GGGWCCC 1 cut(s) 13
Kpn2I TCCGGA 1 cut(s) 71
Kzo9I GATC 3 cut(s) 4, 95, 913
LmnI GCTCC 1 cut(s) 683
Lsp1109I GCAGC 2 cut(s) 338, 779
LweI GCATC 6 cut(s) 338, 422, 552, 747, 965, 993
MaeI CTAG 2 cut(s) 54, 717
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 3 cut(s) 574, 610, 853
MalI GATC 3 cut(s) 6, 97, 915
MboI GATC 3 cut(s) 4, 95, 913
MboII GAAGA 4 cut(s) 183, 409, 500, 829
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 3 cut(s) 407, 669, 883
Mph1103I ATGCAT 1 cut(s) 978
MroI TCCGGA 1 cut(s) 71
MslI CAYNNNNRTG 1 cut(s) 479
MspI CCGG 2 cut(s) 72, 179
MspR9I CCNGG 4 cut(s) 102, 246, 342, 387
Mva1269I GAATGC 2 cut(s) 105, 903
MvaI CCWGG 4 cut(s) 102, 246, 342, 387
MvnI CGCG 1 cut(s) 947
MwoI GCNNNNNNNGC 6 cut(s) 184, 332, 560, 702, 941, 953
NcoI CCATGG 1 cut(s) 208
NdeI CATATG 1 cut(s) 616
NdeII GATC 3 cut(s) 4, 95, 913
NlaIII CATG 5 cut(s) 95, 212, 807, 872, 989
NlaIV GGNNCC 5 cut(s) 14, 15, 70, 240, 562
NmuCI GTSAC 3 cut(s) 574, 610, 853
NsiI ATGCAT 1 cut(s) 978
PceI AGGCCT 1 cut(s) 277
PctI GAATGC 2 cut(s) 105, 903
PfeI GAWTC 1 cut(s) 251
PflMI CCANNNNNTGG 1 cut(s) 392
PkrI GCNGC 4 cut(s) 328, 381, 769, 946
PpuMI RGGWCCY 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 13
Psp6I CCWGG 4 cut(s) 100, 244, 340, 385
PspFI CCCAGC 1 cut(s) 443
PspGI CCWGG 4 cut(s) 100, 244, 340, 385
PspN4I GGNNCC 5 cut(s) 14, 15, 70, 240, 562
PspPI GGNCC 4 cut(s) 13, 69, 290, 527
PspPPI RGGWCCY 1 cut(s) 13
PstI CTGCAG 1 cut(s) 328
PstNI CAGNNNCTG 3 cut(s) 332, 392, 967
RsaI GTAC 2 cut(s) 346, 607
RsaNI GTAC 2 cut(s) 345, 606
RseI CAYNNNNRTG 1 cut(s) 479
SatI GCNGC 4 cut(s) 327, 380, 768, 945
Sau3AI GATC 3 cut(s) 4, 95, 913
Sau96I GGNCC 4 cut(s) 13, 69, 290, 527
ScrFI CCNGG 4 cut(s) 102, 246, 342, 387
SduI GDGCHC 1 cut(s) 229
SfaNI GCATC 6 cut(s) 338, 422, 552, 747, 965, 993
SfcI CTRYAG 2 cut(s) 324, 843
SinI GGWCC 3 cut(s) 13, 69, 290
SmiMI CAYNNNNRTG 1 cut(s) 479
Sse9I AATT 3 cut(s) 407, 669, 883
SseBI AGGCCT 1 cut(s) 277
SsiI CCGC 4 cut(s) 379, 423, 696, 945
SspI AATATT 1 cut(s) 148
SspMI CTAG 2 cut(s) 54, 717
StuI AGGCCT 1 cut(s) 277
StyD4I CCNGG 4 cut(s) 100, 244, 340, 385
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 9 cut(s) 81, 235, 304, 349, 574, 610, 627, 877, 983
TaiI ACGT 1 cut(s) 495
TaqI TCGA 1 cut(s) 280
TasI AATT 3 cut(s) 407, 669, 883
TauI GCSGC 2 cut(s) 382, 947
TfiI GAWTC 1 cut(s) 251
TscAI CASTG 3 cut(s) 84, 486, 972
TseFI GTSAC 3 cut(s) 574, 610, 853
TseI GCWGC 2 cut(s) 326, 767
Tsp45I GTSAC 3 cut(s) 574, 610, 853
TspDTI ATGAA 5 cut(s) 98, 192, 297, 681, 927
TspRI CASTG 3 cut(s) 84, 486, 972
Van91I CCANNNNNTGG 1 cut(s) 392
VneI GTGCAC 1 cut(s) 225
VpaK11BI GGWCC 3 cut(s) 13, 69, 290
XbaI TCTAGA 1 cut(s) 53
XspI CTAG 2 cut(s) 54, 717
Zsp2I ATGCAT 1 cut(s) 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.