Rorug01G0243500

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0009833
Physical Location & Seq
Reverse (-)
34887590 .. 34892653
5064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0243500.1

Sequence Viewer

Length: 1668 bp
ATGTCTTCATCAGATCCTCCCCCGGCAGCTCAACGTCGATTAGCTCAATATCATCCTACCATTTGGGGTCCCGACCTCATCGATTCCTTCACCACTCACTACACTTATGAATCACATGCCACACGTTTGGAGGGTCTGAAGAAGAATGTAGCCAAAGCCTTAGTCTCATCCAGTACTAAAGACGATGCTTGCTCCGTTTTGAAGCTTATAGCCTCGGTACAGCGGTTGGGAGTAGCATACCACTTCCGAAATGAGATCGAATCAGCTCTAGTTACTCTTCTTTCTTCCACTAGCACCCTTAGTACCACAAATGATCTTCATACAGCTGCCTTGCAATTTCAAATACTAAGAGAAAATGGTATATCAGTAAGCTCAGATTTGTTTAACAAGTTTAGAAGTGGGGAAGGAAGCTTCAAAGATAGCTTAAGCAACGACGTGGAGGGACTTTTGAGTTTGTATGAAGCCTCACACCTTGGAATGCCAGGAGAAGATGTTCTGGAGGAAGCCAAGAGATTTAGTTCAAAAAATTTGAAACAATCCATGGCAACAATACTGAACGACGATAATCTACTGAAGCGAGTAGAGCAATCATTGCAAACCCCTGTTCACTGGAGGATGCGGAGGTTAGAAGCTCTGAACTTCATCGATATGTACCAAAGGGACGACTCTAAGAACCTGGCACTGCTGGAGTTGGCGAAATTGGACTATAATCTTGTTCAATCAGTATATCAAACTGAGATAAAGGAGCTCTCTAGGTGGTGGAGAGAGTTGGATTTTAAATCGAAGGCAAGTTTCTCAAGGGACAGACTGCCAGAGAACTACTTGTGGGCAATGGGGATTAGTCAGGAGCCCCAATCCTCAGAATGCAGGATAGGCCTCACCAAGTTTGTGTGCATATTAACAGTCATTGATGACATGTATGATGTATATGGATTCCTGGATGAGCTTGAGTGCTTTACAGATGCAGTAATTCAATGGAATATGGAAGCAAAGGAGGTACTTCCCGAGTACATGAAGCCGGTCTATATTGCCATGCTCGAGTTTGGCAATGAATTGTCTGATAACGCATTCAAAAATACTGGTTTAAACCCCCTCCCTTATATTAAGAACGAGTGGGTTAATCTTTGTACAGCCTATATGGTGGAAGCACGGTGGTTCTATGGCGGATACACACCAACTCTTGAAGAGTATTTAAAGAATGCATGGACTTCAGTGGGTGGTCCTGGAGCAATGCTCCATGCTTATTTGTTAACCGAAGGATGCCAACTAACAGAAGCTTCACTCGAGTCCTTTAACCATGGCTTTCAACTGATATATTGGTCATCCATCTTGACTCGACTCAGTGACGATCTGAGCACTTCCAAGGCTGAGGGCGAGAGAGGTGATGTCGCAAAATCTATTCAGTGCTACATGGAAGAAAAAGGTGTATCGGAAGGAGAAGCACAAGATTACATAAAGGGTTTAATCAGCTATGCATGGAAGAAAATCAACCAAGAGAGTGCCAAAACTAGCATACCAAAATCCATTGTGAACCTGTCTCTGAATATGGCTCGGACTGCTCACTGCATTTTTGAACATGGGGATGGCATTGGAAACTCAATTGGGGTGACTAAGGATCGTTTGATCTCTTTGATTGCAAATCCTATCCCCCTCGATGATGATAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

555

Amino Acids

62.67

Weight (kDa)

5.11

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 22 - 198 1.8e-48 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 255 - 495 1.2e-79 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 295 - 495 4e-51 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 223, 619, 1164
AclWI GGATC 2 cut(s) 8, 1623
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 3 cut(s) 158, 593, 1194
AfaI GTAC 7 cut(s) 175, 219, 304, 653, 999, 1010, 1129
AflII CTTAAG 1 cut(s) 424
AflIII ACRYGT 2 cut(s) 122, 915
AjiI CACGTC 1 cut(s) 436
AjnI CCWGG 4 cut(s) 481, 675, 936, 1222
AloI GAACNNNNNNTCC 2 cut(s) 477, 509
Alw21I GWGCWC 2 cut(s) 750, 1358
Alw26I GTCTC 2 cut(s) 169, 1542
AlwI GGATC 2 cut(s) 8, 1623
Ama87I CYCGRG 3 cut(s) 1004, 1037, 1283
AoxI GGCC 1 cut(s) 874
ApeKI GCWGC 2 cut(s) 26, 326
ApoI RAATTY 1 cut(s) 526
ArsI GACNNNNNNTTYG 2 cut(s) 147, 179
Asp700I GAANNNNTTC 1 cut(s) 492
AspS9I GGNCC 2 cut(s) 68, 1220
AsuC2I CCSGG 1 cut(s) 23
AsuHPI GGTGA 4 cut(s) 82, 871, 1394, 1618
AvaI CYCGRG 3 cut(s) 1004, 1037, 1283
AvaII GGWCC 2 cut(s) 68, 1220
BaeI ACNNNNGTAYC 2 cut(s) 209, 242
BanII GRGCYC 2 cut(s) 750, 852
Bbv12I GWGCWC 2 cut(s) 750, 1358
BbvCI CCTCAGC 1 cut(s) 1368
BbvI GCAGC 2 cut(s) 38, 313
BccI CCATC 2 cut(s) 1334, 1577
BciT130I CCWGG 4 cut(s) 483, 677, 938, 1224
BciVI GTATCC 1 cut(s) 1160
BcnI CCSGG 1 cut(s) 23
BcoDI GTCTC 2 cut(s) 169, 1542
BfaI CTAG 4 cut(s) 269, 291, 753, 1509
BfrI CTTAAG 1 cut(s) 424
BfuI GTATCC 1 cut(s) 1160
BisI GCNGC 2 cut(s) 27, 327
BlsI GCNGC 2 cut(s) 28, 328
BmcAI AGTACT 1 cut(s) 175
Bme1390I CCNGG 5 cut(s) 23, 483, 677, 938, 1224
Bme18I GGWCC 2 cut(s) 68, 1220
BmeT110I CYCGRG 3 cut(s) 1004, 1037, 1283
BmgBI CACGTC 1 cut(s) 436
BmgT120I GGNCC 2 cut(s) 68, 1220
BmiI GGNNCC 3 cut(s) 69, 70, 849
BmrFI CCNGG 5 cut(s) 23, 483, 677, 938, 1224
BmsI GCATC 4 cut(s) 175, 606, 952, 1250
BplI GAGNNNNNCTC 2 cut(s) 1218, 1250
BpmI CTGGAG 4 cut(s) 518, 631, 707, 1245
Bpu10I CCTNAGC 1 cut(s) 1368
BpuEI CTTGAG 2 cut(s) 781, 968
BpuMI CCSGG 1 cut(s) 23
Bsa29I ATCGAT 2 cut(s) 81, 645
BsaJI CCNNGG 6 cut(s) 21, 213, 472, 540, 1297, 1362
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bse118I RCCGGY 1 cut(s) 1018
Bse1I ACTGG 3 cut(s) 171, 614, 1084
Bse3DI GCAATG 4 cut(s) 590, 837, 1054, 1236
BseBI CCWGG 4 cut(s) 483, 677, 938, 1224
BseCI ATCGAT 2 cut(s) 81, 645
BseDI CCNNGG 6 cut(s) 21, 213, 472, 540, 1297, 1362
BseGI GGATG 7 cut(s) 52, 167, 621, 946, 1265, 1322, 1588
BseMI GCAATG 4 cut(s) 590, 837, 1054, 1236
BseMII CTCAG 6 cut(s) 387, 726, 873, 1343, 1354, 1359
BseNI ACTGG 3 cut(s) 171, 614, 1084
BseXI GCAGC 2 cut(s) 38, 313
BshFI GGCC 1 cut(s) 876
BshVI ATCGAT 2 cut(s) 81, 645
BsiHKAI GWGCWC 2 cut(s) 750, 1358
BsiHKCI CYCGRG 3 cut(s) 1004, 1037, 1283
BsiSI CCGG 2 cut(s) 23, 1019
BslFI GGGAC 4 cut(s) 54, 456, 674, 815
BsmAI GTCTC 2 cut(s) 169, 1542
BsmFI GGGAC 4 cut(s) 54, 456, 674, 815
BsmI GAATGC 4 cut(s) 483, 869, 1067, 1204
BsnI GGCC 1 cut(s) 876
BsoBI CYCGRG 3 cut(s) 1004, 1037, 1283
Bsp1286I GDGCHC 3 cut(s) 750, 852, 1358
Bsp1407I TGTACA 1 cut(s) 1127
Bsp143I GATC 6 cut(s) 13, 255, 313, 1348, 1615, 1623
Bsp19I CCATGG 2 cut(s) 540, 1297
BspACI CCGC 3 cut(s) 223, 619, 1164
BspANI GGCC 1 cut(s) 876
BspCNI CTCAG 6 cut(s) 386, 727, 872, 1344, 1353, 1360
BspDI ATCGAT 2 cut(s) 81, 645
BspLI GGNNCC 3 cut(s) 69, 70, 849
BspPI GGATC 2 cut(s) 8, 1623
BspTI CTTAAG 1 cut(s) 424
BsrDI GCAATG 4 cut(s) 590, 837, 1054, 1236
BsrFI RCCGGY 1 cut(s) 1018
BsrGI TGTACA 1 cut(s) 1127
BsrI ACTGG 3 cut(s) 171, 614, 1084
BssAI RCCGGY 1 cut(s) 1018
BssECI CCNNGG 6 cut(s) 21, 213, 472, 540, 1297, 1362
BssMI GATC 6 cut(s) 13, 255, 313, 1348, 1615, 1623
BssT1I CCWWGG 4 cut(s) 472, 540, 1297, 1362
Bst2UI CCWGG 4 cut(s) 483, 677, 938, 1224
Bst4CI ACNGT 2 cut(s) 904, 1152
Bst6I CTCTTC 2 cut(s) 282, 1179
BstAFI CTTAAG 1 cut(s) 424
BstAPI GCANNNNNTGC 1 cut(s) 592
BstAUI TGTACA 1 cut(s) 1127
BstC8I GCNNGC 1 cut(s) 190
BstDSI CCRYGG 2 cut(s) 540, 1297
BstF5I GGATG 7 cut(s) 52, 167, 621, 946, 1265, 1322, 1588
BstKTI GATC 6 cut(s) 16, 258, 316, 1351, 1618, 1626
BstMAI GTCTC 2 cut(s) 169, 1542
BstMBI GATC 6 cut(s) 13, 255, 313, 1348, 1615, 1623
BstMWI GCNNNNNNNGC 4 cut(s) 583, 592, 873, 1556
BstNI CCWGG 4 cut(s) 483, 677, 938, 1224
BstNSI RCATGY 2 cut(s) 119, 919
BstSCI CCNGG 5 cut(s) 21, 481, 675, 936, 1222
BstV1I GCAGC 2 cut(s) 38, 313
BstX2I RGATCY 1 cut(s) 13
BstXI CCANNNNNNTGG 1 cut(s) 127
BstYI RGATCY 1 cut(s) 13
Bsu15I ATCGAT 2 cut(s) 81, 645
BsuI GTATCC 1 cut(s) 1160
BsuRI GGCC 1 cut(s) 876
BsuTUI ATCGAT 2 cut(s) 81, 645
BtgI CCRYGG 2 cut(s) 540, 1297
BtrI CACGTC 1 cut(s) 436
BtsCI GGATG 7 cut(s) 52, 167, 621, 946, 1265, 1322, 1588
BtsI GCAGTG 2 cut(s) 680, 1561
BtsIMutI CAGTG 6 cut(s) 607, 680, 1218, 1348, 1409, 1561
Cac8I GCNNGC 1 cut(s) 190
Cfr10I RCCGGY 1 cut(s) 1018
Cfr13I GGNCC 2 cut(s) 68, 1220
ClaI ATCGAT 2 cut(s) 81, 645
Csp6I GTAC 7 cut(s) 174, 218, 303, 652, 998, 1009, 1128
CviQI GTAC 7 cut(s) 174, 218, 303, 652, 998, 1009, 1128
DpnI GATC 6 cut(s) 15, 257, 315, 1350, 1617, 1625
DpnII GATC 6 cut(s) 13, 255, 313, 1348, 1615, 1623
DraI TTTAAA 3 cut(s) 778, 1086, 1194
Eam1104I CTCTTC 2 cut(s) 282, 1179
EarI CTCTTC 2 cut(s) 282, 1179
EciI GGCGGA 1 cut(s) 1179
Ecl136II GAGCTC 1 cut(s) 748
Eco130I CCWWGG 4 cut(s) 472, 540, 1297, 1362
Eco147I AGGCCT 1 cut(s) 876
Eco24I GRGCYC 2 cut(s) 750, 852
Eco47I GGWCC 2 cut(s) 68, 1220
Eco53kI GAGCTC 1 cut(s) 748
Eco57I CTGAAG 3 cut(s) 158, 593, 1194
Eco88I CYCGRG 3 cut(s) 1004, 1037, 1283
EcoICRI GAGCTC 1 cut(s) 748
EcoO109I RGGNCCY 1 cut(s) 68
EcoRII CCWGG 4 cut(s) 481, 675, 936, 1222
EcoT14I CCWWGG 4 cut(s) 472, 540, 1297, 1362
EcoT22I ATGCAT 2 cut(s) 1204, 1477
EcoT38I GRGCYC 2 cut(s) 750, 852
ErhI CCWWGG 4 cut(s) 472, 540, 1297, 1362
FaqI GGGAC 4 cut(s) 54, 456, 674, 815
Fnu4HI GCNGC 2 cut(s) 27, 327
FokI GGATG 7 cut(s) 39, 154, 628, 953, 1272, 1309, 1595
FriOI GRGCYC 2 cut(s) 750, 852
Fsp4HI GCNGC 2 cut(s) 27, 327
FspBI CTAG 4 cut(s) 269, 291, 753, 1509
GluI GCNGC 2 cut(s) 27, 327
GsuI CTGGAG 4 cut(s) 518, 631, 707, 1245
HaeIII GGCC 1 cut(s) 876
HapII CCGG 2 cut(s) 23, 1019
HincII GTYRAC 1 cut(s) 1251
HindII GTYRAC 1 cut(s) 1251
HindIII AAGCTT 3 cut(s) 203, 409, 1275
HinfI GANTC 8 cut(s) 83, 110, 260, 665, 933, 1286, 1333, 1338
HpaI GTTAAC 1 cut(s) 1251
HpaII CCGG 2 cut(s) 23, 1019
HphI GGTGA 4 cut(s) 82, 871, 1394, 1618
Hpy166II GTNNAC 3 cut(s) 607, 1251, 1531
Hpy188III TCNNGA 6 cut(s) 71, 497, 845, 1004, 1181, 1330
Hpy8I GTNNAC 3 cut(s) 607, 1251, 1531
Hpy99I CGWCG 3 cut(s) 39, 437, 563
HpyAV CCTTC 5 cut(s) 97, 398, 778, 1250, 1427
HpyCH4III ACNGT 2 cut(s) 904, 1152
HpyCH4IV ACGT 3 cut(s) 34, 124, 435
HpyCH4V TGCA 9 cut(s) 334, 595, 867, 894, 965, 1202, 1475, 1566, 1637
HpyF10VI GCNNNNNNNGC 4 cut(s) 583, 592, 873, 1556
HpySE526I ACGT 3 cut(s) 34, 124, 435
KflI GGGWCCC 1 cut(s) 68
KspAI GTTAAC 1 cut(s) 1251
Kzo9I GATC 6 cut(s) 13, 255, 313, 1348, 1615, 1623
LmnI GCTCC 5 cut(s) 197, 745, 847, 1226, 1239
Lsp1109I GCAGC 2 cut(s) 38, 313
LweI GCATC 4 cut(s) 175, 606, 952, 1250
MaeI CTAG 4 cut(s) 269, 291, 753, 1509
MaeII ACGT 3 cut(s) 34, 124, 435
MaeIII GTNAC 3 cut(s) 271, 1343, 1606
MalI GATC 6 cut(s) 15, 257, 315, 1350, 1617, 1625
MboI GATC 6 cut(s) 13, 255, 313, 1348, 1615, 1623
MboII GAAGA 9 cut(s) 151, 154, 269, 276, 308, 500, 1196, 1427, 1492
MfeI CAATTG 1 cut(s) 1599
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 3 cut(s) 750, 852, 1358
MluCI AATT 6 cut(s) 335, 526, 698, 969, 1052, 1599
MlyI GAGTC 4 cut(s) 659, 1295, 1327, 1332
MmeI TCCRAC 1 cut(s) 750
Mph1103I ATGCAT 2 cut(s) 1204, 1477
MroXI GAANNNNTTC 1 cut(s) 492
MslI CAYNNNNRTG 2 cut(s) 647, 1581
MspA1I CMGCKG 2 cut(s) 223, 326
MspCI CTTAAG 1 cut(s) 424
MspI CCGG 2 cut(s) 23, 1019
MspR9I CCNGG 5 cut(s) 23, 483, 677, 938, 1224
MssI GTTTAAAC 1 cut(s) 1086
MunI CAATTG 1 cut(s) 1599
Mva1269I GAATGC 4 cut(s) 483, 869, 1067, 1204
MvaI CCWGG 4 cut(s) 483, 677, 938, 1224
MwoI GCNNNNNNNGC 4 cut(s) 583, 592, 873, 1556
NciI CCSGG 1 cut(s) 23
NcoI CCATGG 2 cut(s) 540, 1297
NdeII GATC 6 cut(s) 13, 255, 313, 1348, 1615, 1623
NlaIV GGNNCC 3 cut(s) 69, 70, 849
NmuCI GTSAC 2 cut(s) 1343, 1606
NsiI ATGCAT 2 cut(s) 1204, 1477
NspI RCATGY 2 cut(s) 119, 919
PaeR7I CTCGAG 2 cut(s) 1037, 1283
PceI AGGCCT 1 cut(s) 876
PciI ACATGT 1 cut(s) 915
PctI GAATGC 4 cut(s) 483, 869, 1067, 1204
PdmI GAANNNNTTC 1 cut(s) 492
PfeI GAWTC 4 cut(s) 83, 110, 260, 933
PfoI TCCNGGA 2 cut(s) 936, 1222
PkrI GCNGC 2 cut(s) 28, 328
PleI GAGTC 4 cut(s) 659, 1294, 1327, 1332
PmeI GTTTAAAC 1 cut(s) 1086
PpsI GAGTC 4 cut(s) 659, 1294, 1327, 1332
PpuMI RGGWCCY 1 cut(s) 68
PscI ACATGT 1 cut(s) 915
Psp124BI GAGCTC 1 cut(s) 750
Psp5II RGGWCCY 1 cut(s) 68
Psp6I CCWGG 4 cut(s) 481, 675, 936, 1222
PspGI CCWGG 4 cut(s) 481, 675, 936, 1222
PspN4I GGNNCC 3 cut(s) 69, 70, 849
PspPI GGNCC 2 cut(s) 68, 1220
PspPPI RGGWCCY 1 cut(s) 68
PspXI VCTCGAGB 2 cut(s) 1037, 1283
PsuI RGATCY 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 326
RsaI GTAC 7 cut(s) 175, 219, 304, 653, 999, 1010, 1129
RsaNI GTAC 7 cut(s) 174, 218, 303, 652, 998, 1009, 1128
RseI CAYNNNNRTG 2 cut(s) 647, 1581
SacI GAGCTC 1 cut(s) 750
SatI GCNGC 2 cut(s) 27, 327
Sau3AI GATC 6 cut(s) 13, 255, 313, 1348, 1615, 1623
Sau96I GGNCC 2 cut(s) 68, 1220
ScaI AGTACT 1 cut(s) 175
SchI GAGTC 4 cut(s) 659, 1295, 1327, 1332
ScrFI CCNGG 5 cut(s) 23, 483, 677, 938, 1224
SduI GDGCHC 3 cut(s) 750, 852, 1358
SfaNI GCATC 4 cut(s) 175, 606, 952, 1250
Sfr274I CTCGAG 2 cut(s) 1037, 1283
SinI GGWCC 2 cut(s) 68, 1220
SlaI CTCGAG 2 cut(s) 1037, 1283
SmiMI CAYNNNNRTG 2 cut(s) 647, 1581
SmlI CTYRAG 5 cut(s) 424, 796, 947, 1037, 1283
SmoI CTYRAG 5 cut(s) 424, 796, 947, 1037, 1283
Sse9I AATT 6 cut(s) 335, 526, 698, 969, 1052, 1599
SseBI AGGCCT 1 cut(s) 876
SsiI CCGC 3 cut(s) 223, 619, 1164
SspMI CTAG 4 cut(s) 269, 291, 753, 1509
SstI GAGCTC 1 cut(s) 750
StuI AGGCCT 1 cut(s) 876
StyD4I CCNGG 5 cut(s) 21, 481, 675, 936, 1222
StyI CCWWGG 4 cut(s) 472, 540, 1297, 1362
TaaI ACNGT 2 cut(s) 904, 1152
TaiI ACGT 3 cut(s) 37, 127, 438
TaqI TCGA 9 cut(s) 37, 81, 258, 645, 782, 1038, 1284, 1336, 1653
TasI AATT 6 cut(s) 335, 526, 698, 969, 1052, 1599
TatI WGTACW 3 cut(s) 173, 1008, 1127
TfiI GAWTC 4 cut(s) 83, 110, 260, 933
TscAI CASTG 6 cut(s) 614, 687, 1218, 1348, 1409, 1568
TseFI GTSAC 2 cut(s) 1343, 1606
TseI GCWGC 2 cut(s) 26, 326
Tsp45I GTSAC 2 cut(s) 1343, 1606
TspDTI ATGAA 6 cut(s) 123, 308, 474, 631, 1028, 1065
TspGWI ACGGA 1 cut(s) 184
TspRI CASTG 6 cut(s) 614, 687, 1218, 1348, 1409, 1568
Vha464I CTTAAG 1 cut(s) 424
VpaK11BI GGWCC 2 cut(s) 68, 1220
XapI RAATTY 1 cut(s) 526
XceI RCATGY 2 cut(s) 119, 919
XhoI CTCGAG 2 cut(s) 1037, 1283
XmnI GAANNNNTTC 1 cut(s) 492
XspI CTAG 4 cut(s) 269, 291, 753, 1509
ZrmI AGTACT 1 cut(s) 175
Zsp2I ATGCAT 2 cut(s) 1204, 1477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.