FvH4_7g16070

Protein trichome birefringence-like 25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012827
Physical Location & Seq
Forward (+)
13826663 .. 13829909
3247 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g16070.t1

Sequence Viewer

Length: 1374 bp
ATGGTGAAGGAAATGAGGTCTGAGTATTCATCATCAAACCCATTTTCATCACCATCATCATTTCACAAACATAATCATGTGTTTGTGAAGTTTGCAGTCTCTCTTCTGTTAGTGGGTTTGGCTTTTCTACTCTTCTTCTCCGATTCGATTCGATATATCTCAGTGTTAGAGCCACTGCCACCTCCAGTTGAAACAGAGCAAGAATCAGAGGAAACAGAGACCCCTGTTTCTTCTTTCCTTGCTCAAACGCCTAGTTCTCTTCCTCCTATCGATTTTCCTGCAACTAATATTACCCAAGAATCTGAGAATGTAAAATGTGACATTTTCACTGGAGGATGGGTACCAGACCCATCAGGGCCAAGATATACTAATGAGAGTTGCCCTGAGATAGAGTCTCATCAGAATTGTATGAAGAATGGGCGCCCCGATTCGGCCTACTTTTACTGGAGGTGGAATCCAAGAGACTGTGAGTTACCCAAGTTCAATGCACATAGATTTCTTGCTCTAATGAGGAACAAATCATGGGCGTTCATTGGTGATTCAATTTCTCGTAATCATGTCCAGTCATTGCTTTGCCTTCTCTCTCAGGTGGAACAAGCTGTTGAAGTATACCATGACAAAGAATACAGGTCTAAGAGATGGCTCTTCCCTTCTCACAACTTCACACTTTCAGTCATCTGGACTCCATTTCTCGTCGAAGCAGCTATTTTTGAAGACATGAATGGAGTTTCCACTTCAGAGATCCAGCTTTATCTTGACAAACTTGACAAGACATGGACCGATCAATACAACAACTTGGATTATGTAGTCATTTCTGGTGGGAAATGGTTCCTTAAAACTGCAATATACCATGAAAACAACACAGTCAGTGGCTGTCATAACTGCCCCGGAAAGAACTTAACCGAGGTAGGACTGGAACATGCCTATCGCAATGTTCTCCGCCTGGTTTTCAACTTCATCAAGGGTTCAGATCATAAAGCATCTGTATTCTTCAGAACCACCACGCCCGACCACTTTGAGAATGGAGAGTGGTTTAGTGGAGGGTATTGCAATAGAACAGTGCCATTCAAGGAAGGTGAAGTTGATATGAGAGATGTAGACACCATGATGCGTAACATTGAGTTGGAAGAATTCGAGAAGGCTTCAGCCAATGGCCCCGAAGAGGGGGCACGTCTAACGCTTTTGGACACAACACACCTTTCCTTGTTGAGACCTGATGGGCATCCAGGTCCTTACAGGCAGTTCCAGCCATTTGCAAAGGATCAAAATGCCAAAGTTCAGACTGACTGTCTACATTGGTGCTTGCCTGGGCCTATAGACTCCTGGAATGATTTGGTGATGGAATTGTTGATCAACGGCAAAATATATCAATGA

Protein Analysis

458

Amino Acids

52.41

Weight (kDa)

5.35

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 105 - 157 3.8e-22 PMR5 N terminal Domain
PC-Esterase PF13839 158 - 448 5.9e-94 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012827)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01430 AT4G01080
fragaria_vesca FvH4_7g16070
malus_domestica MD01G1077000.v1.1 MD07G1146500.v1.1
prunus_persica Prupe.2G184500_v2.0.a1
pyrus_communis pycom01g10610 pycom07g14580
rosa_chinensis RchiOBHm_Chr1g0359011
rosa_laevigata RLG00000027909
rosa_multiflora Rmu_sc0004044.1_g000006
rosa_roxburghii Rroxscaffold_4G00297460
rosa_rugosa Rorug01G0267100
rosa_samantha Rh1AG280400 Rh1BG247500 Rh1CG264800 Rh1DG275900
rosa_wichuraiana Rw1G024930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 340
AccB1I GGYRCC 2 cut(s) 340, 420
AccI GTMKAC 3 cut(s) 609, 1098, 1291
AciI CCGC 1 cut(s) 940
AclWI GGATC 2 cut(s) 736, 1269
AcsI RAATTY 1 cut(s) 1130
AcuI CTGAAG 3 cut(s) 720, 976, 1128
AcyI GRCGYC 1 cut(s) 421
AfaI GTAC 1 cut(s) 342
AfiI CCNNNNNNNGG 4 cut(s) 430, 1162, 1163, 1164
AgsI TTSAA 7 cut(s) 191, 484, 543, 605, 713, 952, 1069
AhdI GACNNNNNGTC 1 cut(s) 1287
AjiI CACGTC 1 cut(s) 1172
AjnI CCWGG 4 cut(s) 942, 1225, 1306, 1322
AluBI AGCT 3 cut(s) 599, 704, 748
AluI AGCT 3 cut(s) 599, 704, 748
Alw26I GTCTC 5 cut(s) 103, 212, 399, 456, 1204
AlwI GGATC 2 cut(s) 736, 1269
AlwNI CAGNNNCTG 1 cut(s) 873
AoxI GGCC 4 cut(s) 356, 432, 1153, 1310
ApeKI GCWGC 1 cut(s) 701
ApoI RAATTY 1 cut(s) 1130
Asp700I GAANNNNTTC 1 cut(s) 827
Asp718I GGTACC 1 cut(s) 340
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 5 cut(s) 356, 777, 1154, 1229, 1310
AsuC2I CCSGG 1 cut(s) 888
AsuHPI GGTGA 5 cut(s) 16, 42, 548, 1088, 1348
AvaII GGWCC 2 cut(s) 777, 1229
BaeGI GKGCMC 1 cut(s) 1171
BanI GGYRCC 2 cut(s) 340, 420
BbsI GAAGAC 1 cut(s) 720
BbvI GCAGC 1 cut(s) 713
BccI CCATC 6 cut(s) 61, 330, 358, 633, 1211, 1333
BceAI ACGGC 1 cut(s) 1372
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BciT130I CCWGG 4 cut(s) 944, 1227, 1308, 1324
BclI TGATCA 1 cut(s) 1350
BcnI CCSGG 1 cut(s) 888
BcoDI GTCTC 5 cut(s) 103, 212, 399, 456, 1204
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 1 cut(s) 1314
BfoI RGCGCY 1 cut(s) 424
BisI GCNGC 1 cut(s) 702
BlsI GCNGC 1 cut(s) 703
Bme1390I CCNGG 5 cut(s) 888, 944, 1227, 1308, 1324
Bme18I GGWCC 2 cut(s) 777, 1229
BmeRI GACNNNNNGTC 1 cut(s) 1287
BmgBI CACGTC 1 cut(s) 1172
BmgT120I GGNCC 5 cut(s) 356, 777, 1154, 1229, 1310
BmiI GGNNCC 4 cut(s) 342, 422, 830, 1156
BmrFI CCNGG 5 cut(s) 888, 944, 1227, 1308, 1324
BmsI GCATC 3 cut(s) 989, 1098, 1231
BpiI GAAGAC 1 cut(s) 720
BpmI CTGGAG 3 cut(s) 168, 351, 466
BpuMI CCSGG 1 cut(s) 888
Bsa29I ATCGAT 1 cut(s) 270
BsaBI GATNNNNATC 1 cut(s) 1221
BsaHI GRCGYC 1 cut(s) 421
BsaI GGTCTC 2 cut(s) 212, 1204
BsaJI CCNNGG 3 cut(s) 886, 903, 1307
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 4 cut(s) 430, 1162, 1163, 1164
Bse1I ACTGG 5 cut(s) 185, 334, 449, 562, 918
Bse3DI GCAATG 2 cut(s) 566, 937
Bse8I GATNNNNATC 1 cut(s) 1221
BseBI CCWGG 4 cut(s) 944, 1227, 1308, 1324
BseCI ATCGAT 1 cut(s) 270
BseDI CCNNGG 3 cut(s) 886, 903, 1307
BseGI GGATG 2 cut(s) 341, 1222
BseJI GATNNNNATC 1 cut(s) 1221
BseLI CCNNNNNNNGG 4 cut(s) 430, 1162, 1163, 1164
BseMI GCAATG 2 cut(s) 566, 937
BseMII CTCAG 5 cut(s) 12, 174, 294, 375, 599
BseNI ACTGG 5 cut(s) 185, 334, 449, 562, 918
BseSI GKGCMC 1 cut(s) 1171
BseXI GCAGC 1 cut(s) 713
BshFI GGCC 4 cut(s) 358, 434, 1155, 1312
BshNI GGYRCC 2 cut(s) 340, 420
BshVI ATCGAT 1 cut(s) 270
BsiSI CCGG 1 cut(s) 888
BslI CCNNNNNNNGG 4 cut(s) 430, 1162, 1163, 1164
BsmAI GTCTC 5 cut(s) 103, 212, 399, 456, 1204
BsnI GGCC 4 cut(s) 358, 434, 1155, 1312
Bso31I GGTCTC 2 cut(s) 212, 1204
Bsp1286I GDGCHC 1 cut(s) 1171
Bsp143I GATC 5 cut(s) 741, 781, 970, 1261, 1350
BspACI CCGC 1 cut(s) 940
BspANI GGCC 4 cut(s) 358, 434, 1155, 1312
BspCNI CTCAG 5 cut(s) 13, 173, 295, 376, 598
BspDI ATCGAT 1 cut(s) 270
BspLI GGNNCC 4 cut(s) 342, 422, 830, 1156
BspPI GGATC 2 cut(s) 736, 1269
BspQI GCTCTTC 1 cut(s) 650
BspT107I GGYRCC 2 cut(s) 340, 420
BspTNI GGTCTC 2 cut(s) 212, 1204
BsrDI GCAATG 2 cut(s) 566, 937
BsrI ACTGG 5 cut(s) 185, 334, 449, 562, 918
BssECI CCNNGG 3 cut(s) 886, 903, 1307
BssMI GATC 5 cut(s) 741, 781, 970, 1261, 1350
BssNAI GTATAC 1 cut(s) 610
BssNI GRCGYC 1 cut(s) 421
Bst1107I GTATAC 1 cut(s) 610
Bst2UI CCWGG 4 cut(s) 944, 1227, 1308, 1324
Bst4CI ACNGT 4 cut(s) 467, 865, 1060, 1289
Bst6I CTCTTC 5 cut(s) 108, 137, 264, 650, 1155
BstACI GRCGYC 1 cut(s) 421
BstC8I GCNNGC 1 cut(s) 1304
BstDEI CTNAG 6 cut(s) 21, 160, 303, 384, 585, 633
BstF5I GGATG 2 cut(s) 341, 1222
BstH2I RGCGCY 1 cut(s) 424
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 5 cut(s) 744, 784, 973, 1264, 1353
BstMAI GTCTC 5 cut(s) 103, 212, 399, 456, 1204
BstMBI GATC 5 cut(s) 741, 781, 970, 1261, 1350
BstMWI GCNNNNNNNGC 1 cut(s) 1246
BstNI CCWGG 4 cut(s) 944, 1227, 1308, 1324
BstNSI RCATGY 1 cut(s) 923
BstSCI CCNGG 5 cut(s) 886, 942, 1225, 1306, 1322
BstSFI CTRYAG 1 cut(s) 1314
BstSLI GKGCMC 1 cut(s) 1171
BstV1I GCAGC 1 cut(s) 713
BstV2I GAAGAC 1 cut(s) 720
BstX2I RGATCY 1 cut(s) 741
BstYI RGATCY 1 cut(s) 741
BstZ17I GTATAC 1 cut(s) 610
Bsu15I ATCGAT 1 cut(s) 270
BsuRI GGCC 4 cut(s) 358, 434, 1155, 1312
BsuTUI ATCGAT 1 cut(s) 270
BtrI CACGTC 1 cut(s) 1172
BtsCI GGATG 2 cut(s) 341, 1222
BtsI GCAGTG 1 cut(s) 173
BtsIMutI CAGTG 5 cut(s) 168, 173, 327, 874, 1065
Cac8I GCNNGC 1 cut(s) 1304
CaiI CAGNNNCTG 1 cut(s) 873
CfoI GCGC 1 cut(s) 423
Cfr13I GGNCC 5 cut(s) 356, 777, 1154, 1229, 1310
ClaI ATCGAT 1 cut(s) 270
Csp6I GTAC 1 cut(s) 341
CviAII CATG 9 cut(s) 77, 522, 557, 614, 718, 774, 851, 920, 1105
CviQI GTAC 1 cut(s) 341
DdeI CTNAG 6 cut(s) 21, 160, 303, 384, 585, 633
DinI GGCGCC 1 cut(s) 422
DpnI GATC 5 cut(s) 743, 783, 972, 1263, 1352
DpnII GATC 5 cut(s) 741, 781, 970, 1261, 1350
DriI GACNNNNNGTC 1 cut(s) 1287
Eam1104I CTCTTC 5 cut(s) 108, 137, 264, 650, 1155
Eam1105I GACNNNNNGTC 1 cut(s) 1287
EarI CTCTTC 5 cut(s) 108, 137, 264, 650, 1155
EciI GGCGGA 1 cut(s) 929
Eco31I GGTCTC 2 cut(s) 212, 1204
Eco47I GGWCC 2 cut(s) 777, 1229
Eco57I CTGAAG 3 cut(s) 720, 976, 1128
EcoO109I RGGNCCY 1 cut(s) 1229
EcoRI GAATTC 1 cut(s) 1130
EcoRII CCWGG 4 cut(s) 942, 1225, 1306, 1322
EgeI GGCGCC 1 cut(s) 422
EheI GGCGCC 1 cut(s) 422
FaeI CATG 9 cut(s) 80, 525, 560, 617, 721, 777, 854, 923, 1108
FatI CATG 9 cut(s) 76, 521, 556, 613, 717, 773, 850, 919, 1104
FbaI TGATCA 1 cut(s) 1350
FblI GTMKAC 3 cut(s) 609, 1098, 1291
Fnu4HI GCNGC 1 cut(s) 702
FokI GGATG 2 cut(s) 348, 1209
Fsp4HI GCNGC 1 cut(s) 702
FspBI CTAG 1 cut(s) 252
GlaI GCGC 1 cut(s) 422
GluI GCNGC 1 cut(s) 702
GsuI CTGGAG 3 cut(s) 168, 351, 466
HaeII RGCGCY 1 cut(s) 424
HaeIII GGCC 4 cut(s) 358, 434, 1155, 1312
HapII CCGG 1 cut(s) 888
HhaI GCGC 1 cut(s) 423
Hin1I GRCGYC 1 cut(s) 421
Hin1II CATG 9 cut(s) 80, 525, 560, 617, 721, 777, 854, 923, 1108
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HpaII CCGG 1 cut(s) 888
HphI GGTGA 5 cut(s) 16, 42, 548, 1088, 1348
Hpy166II GTNNAC 3 cut(s) 610, 1099, 1292
Hpy188I TCNGA 9 cut(s) 22, 142, 208, 304, 402, 739, 970, 995, 1281
Hpy188III TCNNGA 3 cut(s) 679, 755, 1135
Hpy8I GTNNAC 3 cut(s) 610, 1099, 1292
Hpy99I CGWCG 1 cut(s) 698
HpyAV CCTTC 4 cut(s) 587, 660, 1067, 1132
HpyCH4III ACNGT 4 cut(s) 467, 865, 1060, 1289
HpyCH4IV ACGT 1 cut(s) 1171
HpyCH4V TGCA 6 cut(s) 95, 281, 488, 842, 1050, 1256
HpyF10VI GCNNNNNNNGC 1 cut(s) 1246
HpyF3I CTNAG 6 cut(s) 21, 160, 303, 384, 585, 633
HpySE526I ACGT 1 cut(s) 1171
Hsp92I GRCGYC 1 cut(s) 421
Hsp92II CATG 9 cut(s) 80, 525, 560, 617, 721, 777, 854, 923, 1108
HspAI GCGC 1 cut(s) 421
KasI GGCGCC 1 cut(s) 420
KpnI GGTACC 1 cut(s) 344
Ksp22I TGATCA 1 cut(s) 1350
Kzo9I GATC 5 cut(s) 741, 781, 970, 1261, 1350
LguI GCTCTTC 1 cut(s) 650
Lsp1109I GCAGC 1 cut(s) 713
LweI GCATC 3 cut(s) 989, 1098, 1231
MaeI CTAG 1 cut(s) 252
MaeII ACGT 1 cut(s) 1171
MaeIII GTNAC 3 cut(s) 317, 471, 1112
MalI GATC 5 cut(s) 743, 783, 972, 1263, 1352
MboI GATC 5 cut(s) 741, 781, 970, 1261, 1350
MflI RGATCY 1 cut(s) 741
MhlI GDGCHC 1 cut(s) 1171
MluCI AATT 4 cut(s) 403, 543, 1130, 1343
Mly113I GGCGCC 1 cut(s) 421
MlyI GAGTC 3 cut(s) 401, 676, 1313
MmeI TCCRAC 1 cut(s) 1104
MroXI GAANNNNTTC 1 cut(s) 827
MseI TTAA 2 cut(s) 834, 899
MslI CAYNNNNRTG 2 cut(s) 75, 1106
MspI CCGG 1 cut(s) 888
MspR9I CCNGG 5 cut(s) 888, 944, 1227, 1308, 1324
MvaI CCWGG 4 cut(s) 944, 1227, 1308, 1324
MwoI GCNNNNNNNGC 1 cut(s) 1246
NarI GGCGCC 1 cut(s) 421
NciI CCSGG 1 cut(s) 888
NdeII GATC 5 cut(s) 741, 781, 970, 1261, 1350
NlaIII CATG 9 cut(s) 80, 525, 560, 617, 721, 777, 854, 923, 1108
NlaIV GGNNCC 4 cut(s) 342, 422, 830, 1156
NmuCI GTSAC 1 cut(s) 317
NspI RCATGY 1 cut(s) 923
PciSI GCTCTTC 1 cut(s) 650
PdmI GAANNNNTTC 1 cut(s) 827
PfeI GAWTC 7 cut(s) 143, 148, 203, 299, 428, 454, 539
PfoI TCCNGGA 1 cut(s) 1322
PkrI GCNGC 1 cut(s) 703
PleI GAGTC 3 cut(s) 400, 676, 1313
PluTI GGCGCC 1 cut(s) 424
PpsI GAGTC 3 cut(s) 400, 676, 1313
PpuMI RGGWCCY 1 cut(s) 1229
Psp5II RGGWCCY 1 cut(s) 1229
Psp6I CCWGG 4 cut(s) 942, 1225, 1306, 1322
PspGI CCWGG 4 cut(s) 942, 1225, 1306, 1322
PspN4I GGNNCC 4 cut(s) 342, 422, 830, 1156
PspPI GGNCC 5 cut(s) 356, 777, 1154, 1229, 1310
PspPPI RGGWCCY 1 cut(s) 1229
PstNI CAGNNNCTG 1 cut(s) 873
PsuI RGATCY 1 cut(s) 741
RsaI GTAC 1 cut(s) 342
RsaNI GTAC 1 cut(s) 341
RseI CAYNNNNRTG 2 cut(s) 75, 1106
SapI GCTCTTC 1 cut(s) 650
SaqAI TTAA 2 cut(s) 834, 899
SatI GCNGC 1 cut(s) 702
Sau3AI GATC 5 cut(s) 741, 781, 970, 1261, 1350
Sau96I GGNCC 5 cut(s) 356, 777, 1154, 1229, 1310
SchI GAGTC 3 cut(s) 401, 676, 1313
ScrFI CCNGG 5 cut(s) 888, 944, 1227, 1308, 1324
SduI GDGCHC 1 cut(s) 1171
SfaNI GCATC 3 cut(s) 989, 1098, 1231
SfcI CTRYAG 1 cut(s) 1314
SfoI GGCGCC 1 cut(s) 422
SinI GGWCC 2 cut(s) 777, 1229
SmiMI CAYNNNNRTG 2 cut(s) 75, 1106
Sse9I AATT 4 cut(s) 403, 543, 1130, 1343
SsiI CCGC 1 cut(s) 940
SspDI GGCGCC 1 cut(s) 420
SspI AATATT 1 cut(s) 289
SspMI CTAG 1 cut(s) 252
StyD4I CCNGG 5 cut(s) 886, 942, 1225, 1306, 1322
TaaI ACNGT 4 cut(s) 467, 865, 1060, 1289
TaiI ACGT 1 cut(s) 1174
TaqI TCGA 5 cut(s) 146, 151, 270, 696, 1134
TaqII GACCGA 1 cut(s) 794
TasI AATT 4 cut(s) 403, 543, 1130, 1343
TfiI GAWTC 7 cut(s) 143, 148, 203, 299, 428, 454, 539
Tru1I TTAA 2 cut(s) 834, 899
Tru9I TTAA 2 cut(s) 834, 899
TscAI CASTG 5 cut(s) 168, 180, 334, 874, 1065
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 1 cut(s) 701
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 7 cut(s) 18, 36, 425, 520, 734, 867, 946
TspRI CASTG 5 cut(s) 168, 180, 334, 874, 1065
VpaK11BI GGWCC 2 cut(s) 777, 1229
XapI RAATTY 1 cut(s) 1130
XceI RCATGY 1 cut(s) 923
XcmI CCANNNNNNNNNTGG 1 cut(s) 1019
XmiI GTMKAC 3 cut(s) 609, 1098, 1291
XmnI GAANNNNTTC 1 cut(s) 827
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.