Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0012827

Physical Location & Seq

Genomic Coordinates Forward (+)

18359707 .. 18362786

Gene Structure 3080 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1077000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1374 bp

ATGGTGAAAGAAATGAGGGTTCAATCGAACCCAATATTGTCATTCCACAAAAACAGCCATGTATTTGTGAAGTTTGCAGTCTGCTTTGTCTTAGTTGGTCTCGCTTTTCGACTCTTGTTTTCCGATTCTGCACTTTCTATCGGATTAAATTTACCAGTAGAGCCACCTTCTCTTGCAGCTGCAGACCAACAAGAAACAGAATCTTCCTCCTCTTCTGCTTCTGATTCTTCTTTATTTCCTGTCCAAACTCAAAACTCTACAATTGTTGATTTTCCTGGAAATAACTCTCAAACATATGGCAATGTTGCAGTAAAGTGCGACCTTTTTACTGGAGATTGGGTACCAGACCCATCAGGTCCAAGATACACTAACGAAAGCTGCCATGTGATGGAATCTCATCAGAATTGTATGAAGAACGGTCGCCCCGATTCCGAGTACTTTTACTGGAGGTGGAGTCCAAGAGACTGTGAGCTACCCAAGTTCAATCCGCAGAGTTTTCTTGGTACGATGAGGAACAAATCATGGGCCTTCATTGGCGATTCGATTTCGCGGAACCATGTCCAGTCATTGCTTTGCATTCTCTCTCAAGTGGAGCAAGCTGTTGAGGTATACCATGACAAAGAATACAGGTCCAAAAGATGGTTTTTCCCCTCTTACAACTTCACCCTTTCGGTAATTTGGACTCCGTTTCTCGTCAAAGCGGGTATTTTCGAAGACATGAATGGAGTTTCTACTTCCGAAATCCAGCTTCATCTTGACGAGCTTGACAAGACATGGACCGATCAGTATAGCAGCTTGGATTACGTGGTGATTGCCGGAGGGAAATGGTTTCTTAAGACTGCAATTTACCATGAAAGCAACACGGTCAGCGGCTGCCATTACTGTCCCGGAAAGAACTTGACAGAGTTAGGGTTTGACTACGCATATCGCAGTGCCCTCCGTCTGGTTTTCAATTTCATCACTCGTTCGGATCATAAAGCGTTTGTTTTCTTGAGAACCACCACACCTGACCACTTCGAGAACGGAGAGTGGTTCAGTGGGGGGTACTGCAATAGATCAGTGCCATTCAAAGAGGGTGAGGTTGACATAAAAGATGTCGATGCTGCAATGCGAAATATCGAGTTGGAAGAATTTGAGAAGGCTACAGCCCAAGGATCGGAGAAGGGAGTAAACTTTAAACTACTGGACACAACCTACCTTTCACTACTTAGACCTGATGGGCACCCAGGTCCATACAGGCAGTTCCAGCCATTTGCAAAGGATAAGAATGCCAAAGTTCAGAATGATTGCCTACATTGGTGCTTGCCTGGGCCAATAGACTCTTGGAATGATTTGGTTATGGAATTGTTGGTCAATGGCGGAAAATATCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008374 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

458

Amino Acids

52.01

Weight (kDa)

5.65

Isoelectric Point (pI)

41.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	105 - 157	8.1e-22	PMR5 N terminal Domain
PC-Esterase	PF13839	158 - 448	5.1e-93	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012827)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01430 AT4G01080
fragaria_vesca	FvH4_7g16070
malus_domestica	MD01G1077000.v1.1 MD07G1146500.v1.1
prunus_persica	Prupe.2G184500_v2.0.a1
pyrus_communis	pycom01g10610 pycom07g14580
rosa_chinensis	RchiOBHm_Chr1g0359011
rosa_laevigata	RLG00000027909
rosa_multiflora	Rmu_sc0004044.1_g000006
rosa_roxburghii	Rroxscaffold_4G00297460
rosa_rugosa	Rorug01G0267100
rosa_samantha	Rh1AG280400 Rh1BG247500 Rh1CG264800 Rh1DG275900
rosa_wichuraiana	Rw1G024930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	340
AccB1I	GGYRCC	2 cut(s)	340, 1221
AccB7I	CCANNNNNTGG	2 cut(s)	388, 639
AccI	GTMKAC	1 cut(s)	609
AccII	CGCG	1 cut(s)	550
AciI	CCGC	5 cut(s)	488, 550, 701, 870, 1359
AclWI	GGATC	2 cut(s)	978, 1162
AcsI	RAATTY	2 cut(s)	148, 1130
AfaI	GTAC	4 cut(s)	342, 437, 505, 1046
AfiI	CCNNNNNNNGG	4 cut(s)	388, 639, 943, 1156
AflII	CTTAAG	1 cut(s)	833
AgsI	TTSAA	4 cut(s)	23, 484, 952, 1069
AjnI	CCWGG	3 cut(s)	274, 1225, 1306
AluBI	AGCT	7 cut(s)	179, 378, 472, 599, 748, 763, 795
AluI	AGCT	7 cut(s)	179, 378, 472, 599, 748, 763, 795
Alw26I	GTCTC	2 cut(s)	104, 456
AlwI	GGATC	2 cut(s)	978, 1162
AlwNI	CAGNNNCTG	1 cut(s)	873
AoxI	GGCC	2 cut(s)	525, 1310
ApeKI	GCWGC	6 cut(s)	176, 179, 378, 792, 873, 1103
ApoI	RAATTY	2 cut(s)	148, 1130
Asp718I	GGTACC	1 cut(s)	340
AspS9I	GGNCC	6 cut(s)	356, 525, 630, 777, 1229, 1310
AsuC2I	CCSGG	1 cut(s)	888
AsuHPI	GGTGA	4 cut(s)	16, 655, 820, 1088
AsuII	TTCGAA	1 cut(s)	711
AvaII	GGWCC	4 cut(s)	356, 630, 777, 1229
BaeGI	GKGCMC	2 cut(s)	937, 1224
BanI	GGYRCC	2 cut(s)	340, 1221
BbsI	GAAGAC	1 cut(s)	720
BbvI	GCAGC	6 cut(s)	166, 188, 365, 804, 860, 1090
BccI	CCATC	4 cut(s)	358, 382, 633, 1211
BciT130I	CCWGG	3 cut(s)	276, 1227, 1308
BcnI	CCSGG	1 cut(s)	888
BcoDI	GTCTC	2 cut(s)	104, 456
BfmI	CTRYAG	2 cut(s)	180, 1143
BfrI	CTTAAG	1 cut(s)	833
BisI	GCNGC	7 cut(s)	177, 180, 379, 793, 871, 874, 1104
BlsI	GCNGC	7 cut(s)	178, 181, 380, 794, 872, 875, 1105
BmcAI	AGTACT	1 cut(s)	437
Bme1390I	CCNGG	4 cut(s)	276, 888, 1227, 1308
Bme18I	GGWCC	4 cut(s)	356, 630, 777, 1229
BmgT120I	GGNCC	6 cut(s)	356, 525, 630, 777, 1229, 1310
BmiI	GGNNCC	3 cut(s)	342, 554, 1223
BmrFI	CCNGG	4 cut(s)	276, 888, 1227, 1308
BmsI	GCATC	1 cut(s)	1090
BpiI	GAAGAC	1 cut(s)	720
BpmI	CTGGAG	2 cut(s)	351, 466
Bpu14I	TTCGAA	1 cut(s)	711
BpuEI	CTTGAG	2 cut(s)	570, 1012
BpuMI	CCSGG	1 cut(s)	888
Bsa29I	ATCGAT	1 cut(s)	1369
BsaAI	YACGTR	1 cut(s)	805
BsaI	GGTCTC	1 cut(s)	104
BsaJI	CCNNGG	3 cut(s)	1150, 1225, 1307
BsaXI	ACNNNNNCTCC	4 cut(s)	324, 354, 439, 469
Bsc4I	CCNNNNNNNGG	4 cut(s)	388, 639, 943, 1156
Bse1I	ACTGG	5 cut(s)	155, 334, 449, 562, 1188
Bse3DI	GCAATG	3 cut(s)	307, 566, 1113
BseBI	CCWGG	3 cut(s)	276, 1227, 1308
BseCI	ATCGAT	1 cut(s)	1369
BseDI	CCNNGG	3 cut(s)	1150, 1225, 1307
BseLI	CCNNNNNNNGG	4 cut(s)	388, 639, 943, 1156
BseMI	GCAATG	3 cut(s)	307, 566, 1113
BseNI	ACTGG	5 cut(s)	155, 334, 449, 562, 1188
BseRI	GAGGAG	1 cut(s)	199
BseSI	GKGCMC	2 cut(s)	937, 1224
BseXI	GCAGC	6 cut(s)	166, 188, 365, 804, 860, 1090
BsgI	GTGCAG	1 cut(s)	114
Bsh1236I	CGCG	1 cut(s)	550
Bsh1285I	CGRYCG	1 cut(s)	421
BshFI	GGCC	2 cut(s)	527, 1312
BshNI	GGYRCC	2 cut(s)	340, 1221
BshVI	ATCGAT	1 cut(s)	1369
BsiEI	CGRYCG	1 cut(s)	421
BsiSI	CCGG	2 cut(s)	816, 888
BslFI	GGGAC	1 cut(s)	870
BslI	CCNNNNNNNGG	4 cut(s)	388, 639, 943, 1156
BsmAI	GTCTC	2 cut(s)	104, 456
BsmFI	GGGAC	1 cut(s)	870
BsmI	GAATGC	2 cut(s)	576, 1273
BsnI	GGCC	2 cut(s)	527, 1312
Bso31I	GGTCTC	1 cut(s)	104
Bsp119I	TTCGAA	1 cut(s)	711
Bsp1286I	GDGCHC	2 cut(s)	937, 1224
Bsp143I	GATC	4 cut(s)	781, 970, 1055, 1154
BspACI	CCGC	5 cut(s)	488, 550, 701, 870, 1359
BspANI	GGCC	2 cut(s)	527, 1312
BspDI	ATCGAT	1 cut(s)	1369
BspFNI	CGCG	1 cut(s)	550
BspLI	GGNNCC	3 cut(s)	342, 554, 1223
BspMAI	CTGCAG	1 cut(s)	184
BspPI	GGATC	2 cut(s)	978, 1162
BspT104I	TTCGAA	1 cut(s)	711
BspT107I	GGYRCC	2 cut(s)	340, 1221
BspTI	CTTAAG	1 cut(s)	833
BspTNI	GGTCTC	1 cut(s)	104
BsrDI	GCAATG	3 cut(s)	307, 566, 1113
BsrI	ACTGG	5 cut(s)	155, 334, 449, 562, 1188
BssECI	CCNNGG	3 cut(s)	1150, 1225, 1307
BssMI	GATC	4 cut(s)	781, 970, 1055, 1154
BssNAI	GTATAC	1 cut(s)	610
BssT1I	CCWWGG	1 cut(s)	1150
Bst1107I	GTATAC	1 cut(s)	610
Bst2UI	CCWGG	3 cut(s)	276, 1227, 1308
Bst4CI	ACNGT	4 cut(s)	419, 467, 865, 884
Bst6I	CTCTTC	1 cut(s)	217
BstAFI	CTTAAG	1 cut(s)	833
BstBAI	YACGTR	1 cut(s)	805
BstBI	TTCGAA	1 cut(s)	711
BstC8I	GCNNGC	2 cut(s)	597, 1304
BstDEI	CTNAG	2 cut(s)	91, 1208
BstFNI	CGCG	1 cut(s)	550
BstKTI	GATC	4 cut(s)	784, 973, 1058, 1157
BstMAI	GTCTC	2 cut(s)	104, 456
BstMBI	GATC	4 cut(s)	781, 970, 1055, 1154
BstMCI	CGRYCG	1 cut(s)	421
BstMWI	GCNNNNNNNGC	1 cut(s)	1246
BstNI	CCWGG	3 cut(s)	276, 1227, 1308
BstSCI	CCNGG	4 cut(s)	274, 886, 1225, 1306
BstSFI	CTRYAG	2 cut(s)	180, 1143
BstSLI	GKGCMC	2 cut(s)	937, 1224
BstUI	CGCG	1 cut(s)	550
BstV1I	GCAGC	6 cut(s)	166, 188, 365, 804, 860, 1090
BstV2I	GAAGAC	1 cut(s)	720
BstZ17I	GTATAC	1 cut(s)	610
Bsu15I	ATCGAT	1 cut(s)	1369
BsuRI	GGCC	2 cut(s)	527, 1312
BsuTUI	ATCGAT	1 cut(s)	1369
BtsI	GCAGTG	1 cut(s)	937
BtsIMutI	CAGTG	3 cut(s)	937, 1042, 1065
Cac8I	GCNNGC	2 cut(s)	597, 1304
CaiI	CAGNNNCTG	1 cut(s)	873
Cfr13I	GGNCC	6 cut(s)	356, 525, 630, 777, 1229, 1310
ClaI	ATCGAT	1 cut(s)	1369
Csp6I	GTAC	4 cut(s)	341, 436, 504, 1045
CviAII	CATG	8 cut(s)	59, 383, 522, 557, 614, 718, 774, 851
CviQI	GTAC	4 cut(s)	341, 436, 504, 1045
DdeI	CTNAG	2 cut(s)	91, 1208
DpnI	GATC	4 cut(s)	783, 972, 1057, 1156
DpnII	GATC	4 cut(s)	781, 970, 1055, 1154
DraI	TTTAAA	1 cut(s)	1177
Eam1104I	CTCTTC	1 cut(s)	217
EarI	CTCTTC	1 cut(s)	217
EciI	GGCGGA	1 cut(s)	1374
Eco130I	CCWWGG	1 cut(s)	1150
Eco31I	GGTCTC	1 cut(s)	104
Eco47I	GGWCC	4 cut(s)	356, 630, 777, 1229
EcoRII	CCWGG	3 cut(s)	274, 1225, 1306
EcoT14I	CCWWGG	1 cut(s)	1150
ErhI	CCWWGG	1 cut(s)	1150
FaeI	CATG	8 cut(s)	62, 386, 525, 560, 617, 721, 777, 854
FaqI	GGGAC	1 cut(s)	870
FatI	CATG	8 cut(s)	58, 382, 521, 556, 613, 717, 773, 850
FauI	CCCGC	1 cut(s)	694
FauNDI	CATATG	1 cut(s)	295
FblI	GTMKAC	1 cut(s)	609
Fnu4HI	GCNGC	7 cut(s)	177, 180, 379, 793, 871, 874, 1104
Fsp4HI	GCNGC	7 cut(s)	177, 180, 379, 793, 871, 874, 1104
GluI	GCNGC	7 cut(s)	177, 180, 379, 793, 871, 874, 1104
GsuI	CTGGAG	2 cut(s)	351, 466
HaeIII	GGCC	2 cut(s)	527, 1312
HapII	CCGG	2 cut(s)	816, 888
Hin1II	CATG	8 cut(s)	62, 386, 525, 560, 617, 721, 777, 854
HincII	GTYRAC	1 cut(s)	1084
HindII	GTYRAC	1 cut(s)	1084
HpaII	CCGG	2 cut(s)	816, 888
HphI	GGTGA	4 cut(s)	16, 655, 820, 1088
Hpy166II	GTNNAC	3 cut(s)	610, 1084, 1171
Hpy188I	TCNGA	9 cut(s)	124, 143, 223, 402, 433, 739, 970, 1159, 1281
Hpy188III	TCNNGA	3 cut(s)	755, 991, 1018
Hpy8I	GTNNAC	3 cut(s)	610, 1084, 1171
HpyAV	CCTTC	4 cut(s)	177, 538, 1132, 1156
HpyCH4III	ACNGT	4 cut(s)	419, 467, 865, 884
HpyCH4IV	ACGT	1 cut(s)	804
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1246
HpyF3I	CTNAG	2 cut(s)	91, 1208
HpySE526I	ACGT	1 cut(s)	804
Hsp92II	CATG	8 cut(s)	62, 386, 525, 560, 617, 721, 777, 854
KpnI	GGTACC	1 cut(s)	344
Kzo9I	GATC	4 cut(s)	781, 970, 1055, 1154
LmnI	GCTCC	1 cut(s)	592
Lsp1109I	GCAGC	6 cut(s)	166, 188, 365, 804, 860, 1090
LweI	GCATC	1 cut(s)	1090
MaeII	ACGT	1 cut(s)	804
MalI	GATC	4 cut(s)	783, 972, 1057, 1156
MboI	GATC	4 cut(s)	781, 970, 1055, 1154
MboII	GAAGA	6 cut(s)	195, 204, 219, 424, 725, 1139
MfeI	CAATTG	1 cut(s)	261
MhlI	GDGCHC	2 cut(s)	937, 1224
MluCI	AATT	8 cut(s)	148, 261, 403, 675, 843, 952, 1130, 1343
MlyI	GAGTC	4 cut(s)	105, 463, 676, 1313
MmeI	TCCRAC	1 cut(s)	1104
MseI	TTAA	3 cut(s)	146, 834, 1176
MspA1I	CMGCKG	2 cut(s)	179, 870
MspCI	CTTAAG	1 cut(s)	833
MspI	CCGG	2 cut(s)	816, 888
MspR9I	CCNGG	4 cut(s)	276, 888, 1227, 1308
MunI	CAATTG	1 cut(s)	261
Mva1269I	GAATGC	2 cut(s)	576, 1273
MvaI	CCWGG	3 cut(s)	276, 1227, 1308
MvnI	CGCG	1 cut(s)	550
MwoI	GCNNNNNNNGC	1 cut(s)	1246
NciI	CCSGG	1 cut(s)	888
NdeI	CATATG	1 cut(s)	295
NdeII	GATC	4 cut(s)	781, 970, 1055, 1154
NlaIII	CATG	8 cut(s)	62, 386, 525, 560, 617, 721, 777, 854
NlaIV	GGNNCC	3 cut(s)	342, 554, 1223
NspV	TTCGAA	1 cut(s)	711
PcsI	WCGNNNNNNNCGW	1 cut(s)	423
PctI	GAATGC	2 cut(s)	576, 1273
PfeI	GAWTC	6 cut(s)	125, 200, 224, 392, 428, 539
PflMI	CCANNNNNTGG	2 cut(s)	388, 639
PfoI	TCCNGGA	2 cut(s)	274, 886
PkrI	GCNGC	7 cut(s)	178, 181, 380, 794, 872, 875, 1105
PleI	GAGTC	4 cut(s)	105, 462, 676, 1313
PpsI	GAGTC	4 cut(s)	105, 462, 676, 1313
Ppu21I	YACGTR	1 cut(s)	805
Psp6I	CCWGG	3 cut(s)	274, 1225, 1306
PspGI	CCWGG	3 cut(s)	274, 1225, 1306
PspN4I	GGNNCC	3 cut(s)	342, 554, 1223
PspPI	GGNCC	6 cut(s)	356, 525, 630, 777, 1229, 1310
PstI	CTGCAG	1 cut(s)	184
PstNI	CAGNNNCTG	1 cut(s)	873
PvuII	CAGCTG	1 cut(s)	179
RsaI	GTAC	4 cut(s)	342, 437, 505, 1046
RsaNI	GTAC	4 cut(s)	341, 436, 504, 1045
SaqAI	TTAA	3 cut(s)	146, 834, 1176
SatI	GCNGC	7 cut(s)	177, 180, 379, 793, 871, 874, 1104
Sau3AI	GATC	4 cut(s)	781, 970, 1055, 1154
Sau96I	GGNCC	6 cut(s)	356, 525, 630, 777, 1229, 1310
ScaI	AGTACT	1 cut(s)	437
SchI	GAGTC	4 cut(s)	105, 463, 676, 1313
ScrFI	CCNGG	4 cut(s)	276, 888, 1227, 1308
SduI	GDGCHC	2 cut(s)	937, 1224
SfaNI	GCATC	1 cut(s)	1090
SfcI	CTRYAG	2 cut(s)	180, 1143
SfuI	TTCGAA	1 cut(s)	711
SinI	GGWCC	4 cut(s)	356, 630, 777, 1229
SmlI	CTYRAG	3 cut(s)	585, 833, 991
SmoI	CTYRAG	3 cut(s)	585, 833, 991
Sse9I	AATT	8 cut(s)	148, 261, 403, 675, 843, 952, 1130, 1343
SsiI	CCGC	5 cut(s)	488, 550, 701, 870, 1359
SspI	AATATT	1 cut(s)	36
StyD4I	CCNGG	4 cut(s)	274, 886, 1225, 1306
StyI	CCWWGG	1 cut(s)	1150
TaaI	ACNGT	4 cut(s)	419, 467, 865, 884
TaiI	ACGT	1 cut(s)	807
TaqI	TCGA	8 cut(s)	26, 109, 542, 711, 1017, 1098, 1119, 1369
TaqII	GACCGA	1 cut(s)	794
TasI	AATT	8 cut(s)	148, 261, 403, 675, 843, 952, 1130, 1343
TatI	WGTACW	1 cut(s)	435
TauI	GCSGC	1 cut(s)	873
TfiI	GAWTC	6 cut(s)	125, 200, 224, 392, 428, 539
Tru1I	TTAA	3 cut(s)	146, 834, 1176
Tru9I	TTAA	3 cut(s)	146, 834, 1176
TscAI	CASTG	3 cut(s)	937, 1042, 1065
TseI	GCWGC	6 cut(s)	176, 179, 378, 792, 873, 1103
TspDTI	ATGAA	6 cut(s)	425, 520, 734, 740, 867, 946
TspGWI	ACGGA	3 cut(s)	675, 929, 1038
TspRI	CASTG	3 cut(s)	937, 1042, 1065
Van91I	CCANNNNNTGG	2 cut(s)	388, 639
Vha464I	CTTAAG	1 cut(s)	833
VpaK11BI	GGWCC	4 cut(s)	356, 630, 777, 1229
XapI	RAATTY	2 cut(s)	148, 1130
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1320
XmiI	GTMKAC	1 cut(s)	609
ZrmI	AGTACT	1 cut(s)	437

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.