Rh1DG275900

Protein trichome birefringence-like 25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0012827
Physical Location & Seq
Forward (+)
49636132 .. 49640562
4431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG275900.1

Sequence Viewer

Length: 1380 bp
ATGTTTTGGTCGCCGGCGACGGTGACTACAGAGACAAATTCTTCGCTTGGGTCTCCGGCGGAGGCGAGTTCTTGGGCCTTCGGCCAGAACGGAGCCGGGATGAGAATGAGTTTGCAATTTTTGGTGGGTTTGGCTTTTCGCTTCTCCGATTCGATTCGATTAGTCTCAGTACTACAGCCATTGCCACCTTCAATTGAAACAGAGCAAGAACCAGAGGCAACAGAGTCCCCTGTTTCTTCTTTCCTTGCTCAAACGCCTAGTTCTTCTCCAACTATTGATTTTCCTGCAACTAATATCACCCAAAATTCTGAGAATGTAAAATGTGACATTTTCACTGGAGAATGGGTACCAGACCCATCAGGGCCAAGATACACCAACGAGAGCTGCCCTGTGATTGAGTCTCATCAGAATTGTATGAAGAATGGTCGCCCCGATTCGGCCTACTTTTACTGGAGGTGGAATCCAAGAGACTGTGAGCTCCCCAGATTCAATGCACAGAGGTTTCTTGCTCTAATGAGGAACAAGTCATGGGCATTCATCGGCGATTCAATTTCTCGTAACCATGTCCAGTCATTGCTTTGCCTACTCTCTCAGGTGGAACAAGCTGTTGAGGTATACCATGACAAAGAATACAGGTCTAAGAGATGGCTCTTCCCTTCTCACAACTTCACACTTTCAGTCATCTGGACTCCTTTCCTTGTCAAAGCAGCTACTTTTGAAGACATGAATGGAGTTTCCACTTCAGAGATCCAGCTTTATCTTGACAAACTCGATAAGACATGGACTGATCAATACAACAGCTTGGATTACCTGGTCATTTCCGGTGGGAAATGGTTCCTTAAAACTGCAATATACTATGAAAATGACACAGTCAGTGGCTGCCATTACTGCCCCGGAAAGAACTTAACCGAGCTAGGATTTGAACACGCCTATCGCAATGCTCTCCGCCTGGTTTTCAACTTCATCAAGGGCTCAGATCATAAAGCATCTGTATTATTCAGAACCACGACGCCTGACCACTTTGAGAATGGAGAGTGGTTTAGTGGAGGGTATTGCAACAGAACAGTGCCATTCAAAGAAGGTGAGGTTGATATGAAAGATGCAGACACCGTAATGCGTAACATTGAGTTGGAAGAATTCGAGAAGGCTTCGGACAAAGGAGCTGAAGAGGGAGTGCGTTTAAAGCTATTGGATACAACACACCTTTCATTGTTGAGACCCGATGGGCATCCAGGTCCTTACAGGCAGTTCCAGCCATTTGCAAAGGACCAAAATGCCAAAGTTCAGACTGACTGCCTACATTGGTGCTTGCCTGGGCCTATAGACTCCTGGAATGATTTGGTGATGGAAATGTTGATTAACGGCGAAAAATATAAATGA

Protein Analysis

459

Amino Acids

52.26

Weight (kDa)

5.26

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 107 - 159 4.6e-23 PMR5 N terminal Domain
PC-Esterase PF13839 160 - 451 3e-95 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012827)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01430 AT4G01080
fragaria_vesca FvH4_7g16070
malus_domestica MD01G1077000.v1.1 MD07G1146500.v1.1
prunus_persica Prupe.2G184500_v2.0.a1
pyrus_communis pycom01g10610 pycom07g14580
rosa_chinensis RchiOBHm_Chr1g0359011
rosa_laevigata RLG00000027909
rosa_multiflora Rmu_sc0004044.1_g000006
rosa_roxburghii Rroxscaffold_4G00297460
rosa_rugosa Rorug01G0267100
rosa_samantha Rh1AG280400 Rh1BG247500 Rh1CG264800 Rh1DG275900
rosa_wichuraiana Rw1G024930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 346
AccB1I GGYRCC 1 cut(s) 346
AccI GTMKAC 1 cut(s) 615
AciI CCGC 2 cut(s) 59, 946
AclWI GGATC 1 cut(s) 742
AcoI YGGCCR 1 cut(s) 82
AcsI RAATTY 3 cut(s) 37, 304, 1136
AcuI CTGAAG 2 cut(s) 726, 1185
AcyI GRCGYC 1 cut(s) 1010
AfaI GTAC 2 cut(s) 171, 348
AfiI CCNNNNNNNGG 1 cut(s) 436
AgsI TTSAA 8 cut(s) 192, 197, 490, 549, 719, 923, 958, 1075
AjnI CCWGG 5 cut(s) 810, 948, 1231, 1312, 1328
AluBI AGCT 9 cut(s) 384, 478, 605, 710, 754, 801, 913, 1163, 1186
AluI AGCT 9 cut(s) 384, 478, 605, 710, 754, 801, 913, 1163, 1186
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 6 cut(s) 26, 57, 169, 405, 462, 1210
AlwI GGATC 1 cut(s) 742
AlwNI CAGNNNCTG 1 cut(s) 879
AoxI GGCC 5 cut(s) 75, 82, 362, 438, 1316
ApeKI GCWGC 3 cut(s) 384, 707, 879
ApoI RAATTY 3 cut(s) 37, 304, 1136
ArsI GACNNNNNNTTYG 2 cut(s) 25, 57
Asp700I GAANNNNTTC 1 cut(s) 833
Asp718I GGTACC 1 cut(s) 346
AspS9I GGNCC 5 cut(s) 75, 362, 1235, 1267, 1316
AsuC2I CCSGG 2 cut(s) 97, 894
AsuHPI GGTGA 4 cut(s) 34, 289, 1094, 1354
AvaII GGWCC 2 cut(s) 1235, 1267
BanI GGYRCC 1 cut(s) 346
BanII GRGCYC 2 cut(s) 480, 974
BbsI GAAGAC 1 cut(s) 726
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 3 cut(s) 371, 719, 866
BccI CCATC 4 cut(s) 364, 639, 1217, 1339
BceAI ACGGC 1 cut(s) 1378
BciT130I CCWGG 5 cut(s) 812, 950, 1233, 1314, 1330
BciVI GTATCC 1 cut(s) 1186
BclI TGATCA 1 cut(s) 787
BcnI CCSGG 2 cut(s) 97, 894
BcoDI GTCTC 6 cut(s) 26, 57, 169, 405, 462, 1210
BfaI CTAG 2 cut(s) 258, 914
BfmI CTRYAG 3 cut(s) 27, 173, 1320
BfuI GTATCC 1 cut(s) 1186
BisI GCNGC 3 cut(s) 385, 708, 880
BlsI GCNGC 3 cut(s) 386, 709, 881
BmcAI AGTACT 1 cut(s) 171
Bme1390I CCNGG 7 cut(s) 97, 812, 894, 950, 1233, 1314, 1330
Bme18I GGWCC 2 cut(s) 1235, 1267
BmgT120I GGNCC 5 cut(s) 75, 362, 1235, 1267, 1316
BmiI GGNNCC 3 cut(s) 94, 348, 836
BmrFI CCNGG 7 cut(s) 97, 812, 894, 950, 1233, 1314, 1330
BmsI GCATC 3 cut(s) 995, 1090, 1237
BpiI GAAGAC 1 cut(s) 726
BpmI CTGGAG 2 cut(s) 357, 472
BpuMI CCSGG 2 cut(s) 97, 894
BsaBI GATNNNNATC 1 cut(s) 1227
BsaHI GRCGYC 1 cut(s) 1010
BsaI GGTCTC 2 cut(s) 57, 1210
BsaJI CCNNGG 2 cut(s) 892, 1313
BsaWI WCCGGW 1 cut(s) 821
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bsc4I CCNNNNNNNGG 1 cut(s) 436
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 3 cut(s) 340, 455, 568
Bse3DI GCAATG 3 cut(s) 179, 572, 943
Bse8I GATNNNNATC 1 cut(s) 1227
BseBI CCWGG 5 cut(s) 812, 950, 1233, 1314, 1330
BseDI CCNNGG 2 cut(s) 892, 1313
BseGI GGATG 2 cut(s) 105, 1228
BseJI GATNNNNATC 1 cut(s) 1227
BseLI CCNNNNNNNGG 1 cut(s) 436
BseMI GCAATG 3 cut(s) 179, 572, 943
BseMII CTCAG 4 cut(s) 180, 300, 605, 987
BseNI ACTGG 3 cut(s) 340, 455, 568
BseXI GCAGC 3 cut(s) 371, 719, 866
BshFI GGCC 5 cut(s) 77, 84, 364, 440, 1318
BshNI GGYRCC 1 cut(s) 346
BsiHKAI GWGCWC 1 cut(s) 480
BsiSI CCGG 5 cut(s) 14, 56, 96, 822, 894
BslFI GGGAC 1 cut(s) 211
BslI CCNNNNNNNGG 1 cut(s) 436
BsmAI GTCTC 6 cut(s) 26, 57, 169, 405, 462, 1210
BsmFI GGGAC 1 cut(s) 211
BsmI GAATGC 1 cut(s) 533
BsnI GGCC 5 cut(s) 77, 84, 364, 440, 1318
Bso31I GGTCTC 2 cut(s) 57, 1210
Bsp1286I GDGCHC 2 cut(s) 480, 974
Bsp143I GATC 3 cut(s) 747, 787, 976
BspACI CCGC 2 cut(s) 59, 946
BspANI GGCC 5 cut(s) 77, 84, 364, 440, 1318
BspCNI CTCAG 4 cut(s) 179, 301, 604, 986
BspLI GGNNCC 3 cut(s) 94, 348, 836
BspPI GGATC 1 cut(s) 742
BspQI GCTCTTC 1 cut(s) 656
BspT107I GGYRCC 1 cut(s) 346
BspTNI GGTCTC 2 cut(s) 57, 1210
BsrDI GCAATG 3 cut(s) 179, 572, 943
BsrFI RCCGGY 1 cut(s) 13
BsrI ACTGG 3 cut(s) 340, 455, 568
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 892, 1313
BssMI GATC 3 cut(s) 747, 787, 976
BssNAI GTATAC 1 cut(s) 616
BssNI GRCGYC 1 cut(s) 1010
Bst1107I GTATAC 1 cut(s) 616
Bst2UI CCWGG 5 cut(s) 812, 950, 1233, 1314, 1330
Bst4CI ACNGT 5 cut(s) 22, 473, 871, 1066, 1111
Bst6I CTCTTC 2 cut(s) 656, 1161
BstACI GRCGYC 1 cut(s) 1010
BstC8I GCNNGC 2 cut(s) 15, 1310
BstDEI CTNAG 5 cut(s) 166, 309, 591, 639, 973
BstF5I GGATG 2 cut(s) 105, 1228
BstKTI GATC 3 cut(s) 750, 790, 979
BstMAI GTCTC 6 cut(s) 26, 57, 169, 405, 462, 1210
BstMBI GATC 3 cut(s) 747, 787, 976
BstMWI GCNNNNNNNGC 3 cut(s) 888, 1183, 1252
BstNI CCWGG 5 cut(s) 812, 950, 1233, 1314, 1330
BstSCI CCNGG 7 cut(s) 95, 810, 892, 948, 1231, 1312, 1328
BstSFI CTRYAG 3 cut(s) 27, 173, 1320
BstV1I GCAGC 3 cut(s) 371, 719, 866
BstV2I GAAGAC 1 cut(s) 726
BstX2I RGATCY 1 cut(s) 747
BstYI RGATCY 1 cut(s) 747
BstZ17I GTATAC 1 cut(s) 616
BsuI GTATCC 1 cut(s) 1186
BsuRI GGCC 5 cut(s) 77, 84, 364, 440, 1318
BtsCI GGATG 2 cut(s) 105, 1228
BtsIMutI CAGTG 3 cut(s) 333, 880, 1071
Cac8I GCNNGC 2 cut(s) 15, 1310
CaiI CAGNNNCTG 1 cut(s) 879
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 5 cut(s) 75, 362, 1235, 1267, 1316
CseI GACGC 1 cut(s) 1018
CsiI ACCWGGT 1 cut(s) 810
Csp6I GTAC 2 cut(s) 170, 347
CviAII CATG 5 cut(s) 528, 563, 620, 724, 780
CviQI GTAC 2 cut(s) 170, 347
DdeI CTNAG 5 cut(s) 166, 309, 591, 639, 973
DpnI GATC 3 cut(s) 749, 789, 978
DpnII GATC 3 cut(s) 747, 787, 976
DraI TTTAAA 1 cut(s) 1182
EaeI YGGCCR 1 cut(s) 82
Eam1104I CTCTTC 2 cut(s) 656, 1161
EarI CTCTTC 2 cut(s) 656, 1161
EciI GGCGGA 2 cut(s) 74, 935
Ecl136II GAGCTC 1 cut(s) 478
Eco24I GRGCYC 2 cut(s) 480, 974
Eco31I GGTCTC 2 cut(s) 57, 1210
Eco47I GGWCC 2 cut(s) 1235, 1267
Eco53kI GAGCTC 1 cut(s) 478
Eco57I CTGAAG 2 cut(s) 726, 1185
EcoICRI GAGCTC 1 cut(s) 478
EcoO109I RGGNCCY 1 cut(s) 1235
EcoRI GAATTC 1 cut(s) 1136
EcoRII CCWGG 5 cut(s) 810, 948, 1231, 1312, 1328
EcoT38I GRGCYC 2 cut(s) 480, 974
FaeI CATG 5 cut(s) 531, 566, 623, 727, 783
FaqI GGGAC 1 cut(s) 211
FatI CATG 5 cut(s) 527, 562, 619, 723, 779
FbaI TGATCA 1 cut(s) 787
FblI GTMKAC 1 cut(s) 615
Fnu4HI GCNGC 3 cut(s) 385, 708, 880
FokI GGATG 2 cut(s) 112, 1215
FriOI GRGCYC 2 cut(s) 480, 974
Fsp4HI GCNGC 3 cut(s) 385, 708, 880
FspBI CTAG 2 cut(s) 258, 914
GluI GCNGC 3 cut(s) 385, 708, 880
GsuI CTGGAG 2 cut(s) 357, 472
HaeIII GGCC 5 cut(s) 77, 84, 364, 440, 1318
HapII CCGG 5 cut(s) 14, 56, 96, 822, 894
HgaI GACGC 1 cut(s) 1018
Hin1I GRCGYC 1 cut(s) 1010
Hin1II CATG 5 cut(s) 531, 566, 623, 727, 783
HpaII CCGG 5 cut(s) 14, 56, 96, 822, 894
HphI GGTGA 4 cut(s) 34, 289, 1094, 1354
Hpy166II GTNNAC 1 cut(s) 616
Hpy188I TCNGA 8 cut(s) 148, 310, 408, 745, 976, 1001, 1153, 1287
Hpy188III TCNNGA 3 cut(s) 685, 761, 1141
Hpy8I GTNNAC 1 cut(s) 616
Hpy99I CGWCG 2 cut(s) 22, 1012
HpyAV CCTTC 5 cut(s) 88, 198, 666, 1073, 1138
HpyCH4III ACNGT 5 cut(s) 22, 473, 871, 1066, 1111
HpyCH4V TGCA 7 cut(s) 115, 287, 494, 848, 1056, 1103, 1262
HpyF10VI GCNNNNNNNGC 3 cut(s) 888, 1183, 1252
HpyF3I CTNAG 5 cut(s) 166, 309, 591, 639, 973
Hsp92I GRCGYC 1 cut(s) 1010
Hsp92II CATG 5 cut(s) 531, 566, 623, 727, 783
KpnI GGTACC 1 cut(s) 350
KroI GCCGGC 1 cut(s) 13
KroNI GCCGGC 1 cut(s) 15
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 3 cut(s) 747, 787, 976
LguI GCTCTTC 1 cut(s) 656
LmnI GCTCC 3 cut(s) 92, 483, 1160
Lsp1109I GCAGC 3 cut(s) 371, 719, 866
LweI GCATC 3 cut(s) 995, 1090, 1237
MabI ACCWGGT 1 cut(s) 810
MaeI CTAG 2 cut(s) 258, 914
MaeIII GTNAC 4 cut(s) 22, 323, 557, 1118
MalI GATC 3 cut(s) 749, 789, 978
MboI GATC 3 cut(s) 747, 787, 976
MboII GAAGA 8 cut(s) 33, 228, 255, 430, 643, 731, 1145, 1178
MfeI CAATTG 1 cut(s) 192
MflI RGATCY 1 cut(s) 747
MhlI GDGCHC 2 cut(s) 480, 974
MluCI AATT 7 cut(s) 37, 116, 192, 304, 409, 549, 1136
MlyI GAGTC 4 cut(s) 233, 407, 682, 1319
MmeI TCCRAC 2 cut(s) 293, 1110
MnlI CCTC 9 cut(s) 55, 208, 447, 492, 510, 604, 1040, 1078, 1162
MreI CGCCGGCG 1 cut(s) 13
MroNI GCCGGC 1 cut(s) 13
MroXI GAANNNNTTC 1 cut(s) 833
MseI TTAA 4 cut(s) 840, 905, 1181, 1359
MslI CAYNNNNRTG 1 cut(s) 1112
MspI CCGG 5 cut(s) 14, 56, 96, 822, 894
MspR9I CCNGG 7 cut(s) 97, 812, 894, 950, 1233, 1314, 1330
MunI CAATTG 1 cut(s) 192
Mva1269I GAATGC 1 cut(s) 533
MvaI CCWGG 5 cut(s) 812, 950, 1233, 1314, 1330
MwoI GCNNNNNNNGC 3 cut(s) 888, 1183, 1252
NaeI GCCGGC 1 cut(s) 15
NciI CCSGG 2 cut(s) 97, 894
NdeII GATC 3 cut(s) 747, 787, 976
NgoMIV GCCGGC 1 cut(s) 13
NlaIII CATG 5 cut(s) 531, 566, 623, 727, 783
NlaIV GGNNCC 3 cut(s) 94, 348, 836
NmuCI GTSAC 2 cut(s) 22, 323
PciSI GCTCTTC 1 cut(s) 656
PctI GAATGC 1 cut(s) 533
PdiI GCCGGC 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 833
PfeI GAWTC 6 cut(s) 149, 154, 434, 460, 486, 545
PflFI GACNNNGTC 1 cut(s) 869
PfoI TCCNGGA 1 cut(s) 1328
PkrI GCNGC 3 cut(s) 386, 709, 881
PleI GAGTC 4 cut(s) 232, 406, 682, 1319
PpsI GAGTC 4 cut(s) 232, 406, 682, 1319
PpuMI RGGWCCY 1 cut(s) 1235
Psp124BI GAGCTC 1 cut(s) 480
Psp5II RGGWCCY 1 cut(s) 1235
Psp6I CCWGG 5 cut(s) 810, 948, 1231, 1312, 1328
PspGI CCWGG 5 cut(s) 810, 948, 1231, 1312, 1328
PspN4I GGNNCC 3 cut(s) 94, 348, 836
PspPI GGNCC 5 cut(s) 75, 362, 1235, 1267, 1316
PspPPI RGGWCCY 1 cut(s) 1235
PstNI CAGNNNCTG 1 cut(s) 879
PsuI RGATCY 1 cut(s) 747
PsyI GACNNNGTC 1 cut(s) 869
RsaI GTAC 2 cut(s) 171, 348
RsaNI GTAC 2 cut(s) 170, 347
RseI CAYNNNNRTG 1 cut(s) 1112
SacI GAGCTC 1 cut(s) 480
SapI GCTCTTC 1 cut(s) 656
SaqAI TTAA 4 cut(s) 840, 905, 1181, 1359
SatI GCNGC 3 cut(s) 385, 708, 880
Sau3AI GATC 3 cut(s) 747, 787, 976
Sau96I GGNCC 5 cut(s) 75, 362, 1235, 1267, 1316
ScaI AGTACT 1 cut(s) 171
SchI GAGTC 4 cut(s) 233, 407, 682, 1319
ScrFI CCNGG 7 cut(s) 97, 812, 894, 950, 1233, 1314, 1330
SduI GDGCHC 2 cut(s) 480, 974
SexAI ACCWGGT 1 cut(s) 810
SfaNI GCATC 3 cut(s) 995, 1090, 1237
SfcI CTRYAG 3 cut(s) 27, 173, 1320
SgrAI CRCCGGYG 1 cut(s) 13
SinI GGWCC 2 cut(s) 1235, 1267
SmiMI CAYNNNNRTG 1 cut(s) 1112
Sse9I AATT 7 cut(s) 37, 116, 192, 304, 409, 549, 1136
SsiI CCGC 2 cut(s) 59, 946
SspMI CTAG 2 cut(s) 258, 914
SstI GAGCTC 1 cut(s) 480
StyD4I CCNGG 7 cut(s) 95, 810, 892, 948, 1231, 1312, 1328
TaaI ACNGT 5 cut(s) 22, 473, 871, 1066, 1111
TaqI TCGA 4 cut(s) 152, 157, 771, 1140
TasI AATT 7 cut(s) 37, 116, 192, 304, 409, 549, 1136
TatI WGTACW 1 cut(s) 169
TfiI GAWTC 6 cut(s) 149, 154, 434, 460, 486, 545
Tru1I TTAA 4 cut(s) 840, 905, 1181, 1359
Tru9I TTAA 4 cut(s) 840, 905, 1181, 1359
TscAI CASTG 3 cut(s) 340, 880, 1071
TseFI GTSAC 2 cut(s) 22, 323
TseI GCWGC 3 cut(s) 384, 707, 879
Tsp45I GTSAC 2 cut(s) 22, 323
TspDTI ATGAA 7 cut(s) 431, 526, 740, 873, 952, 1109, 1197
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 3 cut(s) 340, 880, 1071
Tth111I GACNNNGTC 1 cut(s) 869
VpaK11BI GGWCC 2 cut(s) 1235, 1267
XapI RAATTY 3 cut(s) 37, 304, 1136
XcmI CCANNNNNNNNNTGG 1 cut(s) 1025
XmiI GTMKAC 1 cut(s) 615
XmnI GAANNNNTTC 1 cut(s) 833
XspI CTAG 2 cut(s) 258, 914
ZrmI AGTACT 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.