Rh1BG247500

Protein trichome birefringence-like 25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0012827
Physical Location & Seq
Forward (+)
37472021 .. 37476451
4431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG247500.1

Sequence Viewer

Length: 1395 bp
ATGTTTTGGTCGCCGGCGACGGTGACTACAGAGACAAATTCTTCGCTTGGGTCTCCGGCGGAGGCGAGTTCTTGGGCCTTCGGCCAGAACGGAGCCGGGATGAGAATGAGTTTGCAATTTTTGGTTTGTTTCCTCTTAGTGGGTTTGGCTTTTCGCTTCTCCGATTCGATTCGATTAGTCTCAGTACTACAGCCATTGCCACCTTCAATTGAAACAGAGCAAGAACCAGAGGCAACAGAGTCCCCTGTTTCTTCTTTCCTTGCTCAAACGCCTAGTTCTTCTCCAACTATTGATTTTCCTGCAACTAATATCACCCAAAATTCTGAGAATGTAAAATGTGACATTTTCACTGGAGAATGGGTACCAGACCCATCAGGGCCAAGATACACCAACGAGAGCTGCCCTGTGATTGAGTCTCATCAGAATTGTATGAAGAATGGTCGCCCCGATTCGGCCTACTTTTACTGGAGGTGGAATCCAAGAGACTGTGAGCTCCCCAGATTCAATGCACAGAGGTTTCTTGCTCTAATGAGGAACAAGTCATGGGCATTCATCGGCGATTCAATTTCTCGTAACCATGTCCAGTCATTGCTTTGCCTACTCTCTCAGGTGGAACAAGCTGTTGAGGTATACCATGACAAAGAATACAGGTCTAAGAGATGGCTCTTCCCTTCTCACAACTTCACACTTTCAGTCATCTGGACTCCTTTCCTTGTCAAAGCAGCTACTTTTGAAGACATGAATGGAGTTTCCACTTCAGAGATCCAGCTTTATCTTGACAAACTCGATAAGACATGGACTGATCAATACAACAGCTTGGATTACCTGGTCATTTCCGGTGGGAAATGGTTCCTTAAAACTGCAATATACTATGAAAATGACACAGTCAGTGGCTGCCATTACTGCCCCGGAAAGAACTTAACCGAGCTAGGATTTGAACACGCCTATCGCAATGCTCTCCGCCTGGTTTTCAACTTCATCAAGGGCTCAGATCATAAAGCATCTGTATTATTCAGAACCACGACGCCTGACCACTTTGAGAATGGAGAGTGGTTTAGTGGAGGGTATTGCAACAGAACAGTGCCATTCAAAGAAGGTGAGGTTGATATGAAAGATGCAGACACCGTAATGCGTAACATTGAGTTGGAAGAATTCGAGAAGGCTTCGGACAAAGGAGCTGAAGAGGGAGTGCGTTTAAAGCTATTGGATACAACACACCTTTCATTGTTGAGACCCGATGGGCATCCAGGTCCTTACAGGCAGTTCCAGCCATTTGCAAAGGACCAAAATGCCAAAGTTCAGACTGACTGCCTACATTGGTGCTTGCCTGGGCCTATAGACTCCTGGAATGATTTGGTGATGGAAATGTTGATTAACGGCGAAAAATATAAATGA

Protein Analysis

464

Amino Acids

52.84

Weight (kDa)

5.26

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 112 - 164 4.7e-23 PMR5 N terminal Domain
PC-Esterase PF13839 165 - 456 3.1e-95 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012827)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01430 AT4G01080
fragaria_vesca FvH4_7g16070
malus_domestica MD01G1077000.v1.1 MD07G1146500.v1.1
prunus_persica Prupe.2G184500_v2.0.a1
pyrus_communis pycom01g10610 pycom07g14580
rosa_chinensis RchiOBHm_Chr1g0359011
rosa_laevigata RLG00000027909
rosa_multiflora Rmu_sc0004044.1_g000006
rosa_roxburghii Rroxscaffold_4G00297460
rosa_rugosa Rorug01G0267100
rosa_samantha Rh1AG280400 Rh1BG247500 Rh1CG264800 Rh1DG275900
rosa_wichuraiana Rw1G024930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 361
AccB1I GGYRCC 1 cut(s) 361
AccI GTMKAC 1 cut(s) 630
AciI CCGC 2 cut(s) 59, 961
AclWI GGATC 1 cut(s) 757
AcoI YGGCCR 1 cut(s) 82
AcsI RAATTY 3 cut(s) 37, 319, 1151
AcuI CTGAAG 2 cut(s) 741, 1200
AcyI GRCGYC 1 cut(s) 1025
AfaI GTAC 2 cut(s) 186, 363
AfiI CCNNNNNNNGG 2 cut(s) 139, 451
AgsI TTSAA 8 cut(s) 207, 212, 505, 564, 734, 938, 973, 1090
AjnI CCWGG 5 cut(s) 825, 963, 1246, 1327, 1343
AluBI AGCT 9 cut(s) 399, 493, 620, 725, 769, 816, 928, 1178, 1201
AluI AGCT 9 cut(s) 399, 493, 620, 725, 769, 816, 928, 1178, 1201
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 6 cut(s) 26, 57, 184, 420, 477, 1225
AlwI GGATC 1 cut(s) 757
AlwNI CAGNNNCTG 1 cut(s) 894
AoxI GGCC 5 cut(s) 75, 82, 377, 453, 1331
ApeKI GCWGC 3 cut(s) 399, 722, 894
ApoI RAATTY 3 cut(s) 37, 319, 1151
ArsI GACNNNNNNTTYG 2 cut(s) 25, 57
Asp700I GAANNNNTTC 1 cut(s) 848
Asp718I GGTACC 1 cut(s) 361
AspS9I GGNCC 5 cut(s) 75, 377, 1250, 1282, 1331
AsuC2I CCSGG 2 cut(s) 97, 909
AsuHPI GGTGA 4 cut(s) 34, 304, 1109, 1369
AvaII GGWCC 2 cut(s) 1250, 1282
BanI GGYRCC 1 cut(s) 361
BanII GRGCYC 2 cut(s) 495, 989
BbsI GAAGAC 1 cut(s) 741
Bbv12I GWGCWC 1 cut(s) 495
BbvI GCAGC 3 cut(s) 386, 734, 881
BccI CCATC 4 cut(s) 379, 654, 1232, 1354
BceAI ACGGC 1 cut(s) 1393
BciT130I CCWGG 5 cut(s) 827, 965, 1248, 1329, 1345
BciVI GTATCC 1 cut(s) 1201
BclI TGATCA 1 cut(s) 802
BcnI CCSGG 2 cut(s) 97, 909
BcoDI GTCTC 6 cut(s) 26, 57, 184, 420, 477, 1225
BfaI CTAG 2 cut(s) 273, 929
BfmI CTRYAG 3 cut(s) 27, 188, 1335
BfuI GTATCC 1 cut(s) 1201
BisI GCNGC 3 cut(s) 400, 723, 895
BlsI GCNGC 3 cut(s) 401, 724, 896
BmcAI AGTACT 1 cut(s) 186
Bme1390I CCNGG 7 cut(s) 97, 827, 909, 965, 1248, 1329, 1345
Bme18I GGWCC 2 cut(s) 1250, 1282
BmgT120I GGNCC 5 cut(s) 75, 377, 1250, 1282, 1331
BmiI GGNNCC 3 cut(s) 94, 363, 851
BmrFI CCNGG 7 cut(s) 97, 827, 909, 965, 1248, 1329, 1345
BmsI GCATC 3 cut(s) 1010, 1105, 1252
BpiI GAAGAC 1 cut(s) 741
BpmI CTGGAG 2 cut(s) 372, 487
BpuMI CCSGG 2 cut(s) 97, 909
BsaBI GATNNNNATC 1 cut(s) 1242
BsaHI GRCGYC 1 cut(s) 1025
BsaI GGTCTC 2 cut(s) 57, 1225
BsaJI CCNNGG 2 cut(s) 907, 1328
BsaWI WCCGGW 1 cut(s) 836
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 451
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 3 cut(s) 355, 470, 583
Bse3DI GCAATG 3 cut(s) 194, 587, 958
Bse8I GATNNNNATC 1 cut(s) 1242
BseBI CCWGG 5 cut(s) 827, 965, 1248, 1329, 1345
BseDI CCNNGG 2 cut(s) 907, 1328
BseGI GGATG 2 cut(s) 105, 1243
BseJI GATNNNNATC 1 cut(s) 1242
BseLI CCNNNNNNNGG 2 cut(s) 139, 451
BseMI GCAATG 3 cut(s) 194, 587, 958
BseMII CTCAG 4 cut(s) 195, 315, 620, 1002
BseNI ACTGG 3 cut(s) 355, 470, 583
BseXI GCAGC 3 cut(s) 386, 734, 881
BshFI GGCC 5 cut(s) 77, 84, 379, 455, 1333
BshNI GGYRCC 1 cut(s) 361
BsiHKAI GWGCWC 1 cut(s) 495
BsiSI CCGG 5 cut(s) 14, 56, 96, 837, 909
BslFI GGGAC 1 cut(s) 226
BslI CCNNNNNNNGG 2 cut(s) 139, 451
BsmAI GTCTC 6 cut(s) 26, 57, 184, 420, 477, 1225
BsmFI GGGAC 1 cut(s) 226
BsmI GAATGC 1 cut(s) 548
BsnI GGCC 5 cut(s) 77, 84, 379, 455, 1333
Bso31I GGTCTC 2 cut(s) 57, 1225
Bsp1286I GDGCHC 2 cut(s) 495, 989
Bsp143I GATC 3 cut(s) 762, 802, 991
BspACI CCGC 2 cut(s) 59, 961
BspANI GGCC 5 cut(s) 77, 84, 379, 455, 1333
BspCNI CTCAG 4 cut(s) 194, 316, 619, 1001
BspLI GGNNCC 3 cut(s) 94, 363, 851
BspPI GGATC 1 cut(s) 757
BspQI GCTCTTC 1 cut(s) 671
BspT107I GGYRCC 1 cut(s) 361
BspTNI GGTCTC 2 cut(s) 57, 1225
BsrDI GCAATG 3 cut(s) 194, 587, 958
BsrFI RCCGGY 1 cut(s) 13
BsrI ACTGG 3 cut(s) 355, 470, 583
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 907, 1328
BssMI GATC 3 cut(s) 762, 802, 991
BssNAI GTATAC 1 cut(s) 631
BssNI GRCGYC 1 cut(s) 1025
Bst1107I GTATAC 1 cut(s) 631
Bst2UI CCWGG 5 cut(s) 827, 965, 1248, 1329, 1345
Bst4CI ACNGT 5 cut(s) 22, 488, 886, 1081, 1126
Bst6I CTCTTC 2 cut(s) 671, 1176
BstACI GRCGYC 1 cut(s) 1025
BstC8I GCNNGC 2 cut(s) 15, 1325
BstDEI CTNAG 6 cut(s) 136, 181, 324, 606, 654, 988
BstF5I GGATG 2 cut(s) 105, 1243
BstKTI GATC 3 cut(s) 765, 805, 994
BstMAI GTCTC 6 cut(s) 26, 57, 184, 420, 477, 1225
BstMBI GATC 3 cut(s) 762, 802, 991
BstMWI GCNNNNNNNGC 3 cut(s) 903, 1198, 1267
BstNI CCWGG 5 cut(s) 827, 965, 1248, 1329, 1345
BstSCI CCNGG 7 cut(s) 95, 825, 907, 963, 1246, 1327, 1343
BstSFI CTRYAG 3 cut(s) 27, 188, 1335
BstV1I GCAGC 3 cut(s) 386, 734, 881
BstV2I GAAGAC 1 cut(s) 741
BstX2I RGATCY 1 cut(s) 762
BstYI RGATCY 1 cut(s) 762
BstZ17I GTATAC 1 cut(s) 631
BsuI GTATCC 1 cut(s) 1201
BsuRI GGCC 5 cut(s) 77, 84, 379, 455, 1333
BtsCI GGATG 2 cut(s) 105, 1243
BtsIMutI CAGTG 3 cut(s) 348, 895, 1086
Cac8I GCNNGC 2 cut(s) 15, 1325
CaiI CAGNNNCTG 1 cut(s) 894
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 5 cut(s) 75, 377, 1250, 1282, 1331
CseI GACGC 1 cut(s) 1033
CsiI ACCWGGT 1 cut(s) 825
Csp6I GTAC 2 cut(s) 185, 362
CviAII CATG 5 cut(s) 543, 578, 635, 739, 795
CviQI GTAC 2 cut(s) 185, 362
DdeI CTNAG 6 cut(s) 136, 181, 324, 606, 654, 988
DpnI GATC 3 cut(s) 764, 804, 993
DpnII GATC 3 cut(s) 762, 802, 991
DraI TTTAAA 1 cut(s) 1197
EaeI YGGCCR 1 cut(s) 82
Eam1104I CTCTTC 2 cut(s) 671, 1176
EarI CTCTTC 2 cut(s) 671, 1176
EciI GGCGGA 2 cut(s) 74, 950
Ecl136II GAGCTC 1 cut(s) 493
Eco24I GRGCYC 2 cut(s) 495, 989
Eco31I GGTCTC 2 cut(s) 57, 1225
Eco47I GGWCC 2 cut(s) 1250, 1282
Eco53kI GAGCTC 1 cut(s) 493
Eco57I CTGAAG 2 cut(s) 741, 1200
EcoICRI GAGCTC 1 cut(s) 493
EcoO109I RGGNCCY 1 cut(s) 1250
EcoRI GAATTC 1 cut(s) 1151
EcoRII CCWGG 5 cut(s) 825, 963, 1246, 1327, 1343
EcoT38I GRGCYC 2 cut(s) 495, 989
FaeI CATG 5 cut(s) 546, 581, 638, 742, 798
FaqI GGGAC 1 cut(s) 226
FatI CATG 5 cut(s) 542, 577, 634, 738, 794
FbaI TGATCA 1 cut(s) 802
FblI GTMKAC 1 cut(s) 630
Fnu4HI GCNGC 3 cut(s) 400, 723, 895
FokI GGATG 2 cut(s) 112, 1230
FriOI GRGCYC 2 cut(s) 495, 989
Fsp4HI GCNGC 3 cut(s) 400, 723, 895
FspBI CTAG 2 cut(s) 273, 929
GluI GCNGC 3 cut(s) 400, 723, 895
GsuI CTGGAG 2 cut(s) 372, 487
HaeIII GGCC 5 cut(s) 77, 84, 379, 455, 1333
HapII CCGG 5 cut(s) 14, 56, 96, 837, 909
HgaI GACGC 1 cut(s) 1033
Hin1I GRCGYC 1 cut(s) 1025
Hin1II CATG 5 cut(s) 546, 581, 638, 742, 798
HpaII CCGG 5 cut(s) 14, 56, 96, 837, 909
HphI GGTGA 4 cut(s) 34, 304, 1109, 1369
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 8 cut(s) 163, 325, 423, 760, 991, 1016, 1168, 1302
Hpy188III TCNNGA 3 cut(s) 700, 776, 1156
Hpy8I GTNNAC 1 cut(s) 631
Hpy99I CGWCG 2 cut(s) 22, 1027
HpyAV CCTTC 5 cut(s) 88, 213, 681, 1088, 1153
HpyCH4III ACNGT 5 cut(s) 22, 488, 886, 1081, 1126
HpyCH4V TGCA 7 cut(s) 115, 302, 509, 863, 1071, 1118, 1277
HpyF10VI GCNNNNNNNGC 3 cut(s) 903, 1198, 1267
HpyF3I CTNAG 6 cut(s) 136, 181, 324, 606, 654, 988
Hsp92I GRCGYC 1 cut(s) 1025
Hsp92II CATG 5 cut(s) 546, 581, 638, 742, 798
KpnI GGTACC 1 cut(s) 365
KroI GCCGGC 1 cut(s) 13
KroNI GCCGGC 1 cut(s) 15
Ksp22I TGATCA 1 cut(s) 802
Kzo9I GATC 3 cut(s) 762, 802, 991
LguI GCTCTTC 1 cut(s) 671
LmnI GCTCC 3 cut(s) 92, 498, 1175
Lsp1109I GCAGC 3 cut(s) 386, 734, 881
LweI GCATC 3 cut(s) 1010, 1105, 1252
MabI ACCWGGT 1 cut(s) 825
MaeI CTAG 2 cut(s) 273, 929
MaeIII GTNAC 4 cut(s) 22, 338, 572, 1133
MalI GATC 3 cut(s) 764, 804, 993
MboI GATC 3 cut(s) 762, 802, 991
MboII GAAGA 8 cut(s) 33, 243, 270, 445, 658, 746, 1160, 1193
MfeI CAATTG 1 cut(s) 207
MflI RGATCY 1 cut(s) 762
MhlI GDGCHC 2 cut(s) 495, 989
MluCI AATT 7 cut(s) 37, 116, 207, 319, 424, 564, 1151
MlyI GAGTC 4 cut(s) 248, 422, 697, 1334
MmeI TCCRAC 2 cut(s) 308, 1125
MreI CGCCGGCG 1 cut(s) 13
MroNI GCCGGC 1 cut(s) 13
MroXI GAANNNNTTC 1 cut(s) 848
MseI TTAA 4 cut(s) 855, 920, 1196, 1374
MslI CAYNNNNRTG 1 cut(s) 1127
MspI CCGG 5 cut(s) 14, 56, 96, 837, 909
MspR9I CCNGG 7 cut(s) 97, 827, 909, 965, 1248, 1329, 1345
MunI CAATTG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 548
MvaI CCWGG 5 cut(s) 827, 965, 1248, 1329, 1345
MwoI GCNNNNNNNGC 3 cut(s) 903, 1198, 1267
NaeI GCCGGC 1 cut(s) 15
NciI CCSGG 2 cut(s) 97, 909
NdeII GATC 3 cut(s) 762, 802, 991
NgoMIV GCCGGC 1 cut(s) 13
NlaIII CATG 5 cut(s) 546, 581, 638, 742, 798
NlaIV GGNNCC 3 cut(s) 94, 363, 851
NmuCI GTSAC 2 cut(s) 22, 338
PciSI GCTCTTC 1 cut(s) 671
PctI GAATGC 1 cut(s) 548
PdiI GCCGGC 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 848
PfeI GAWTC 6 cut(s) 164, 169, 449, 475, 501, 560
PflFI GACNNNGTC 1 cut(s) 884
PfoI TCCNGGA 1 cut(s) 1343
PkrI GCNGC 3 cut(s) 401, 724, 896
PleI GAGTC 4 cut(s) 247, 421, 697, 1334
PpsI GAGTC 4 cut(s) 247, 421, 697, 1334
PpuMI RGGWCCY 1 cut(s) 1250
Psp124BI GAGCTC 1 cut(s) 495
Psp5II RGGWCCY 1 cut(s) 1250
Psp6I CCWGG 5 cut(s) 825, 963, 1246, 1327, 1343
PspGI CCWGG 5 cut(s) 825, 963, 1246, 1327, 1343
PspN4I GGNNCC 3 cut(s) 94, 363, 851
PspPI GGNCC 5 cut(s) 75, 377, 1250, 1282, 1331
PspPPI RGGWCCY 1 cut(s) 1250
PstNI CAGNNNCTG 1 cut(s) 894
PsuI RGATCY 1 cut(s) 762
PsyI GACNNNGTC 1 cut(s) 884
RsaI GTAC 2 cut(s) 186, 363
RsaNI GTAC 2 cut(s) 185, 362
RseI CAYNNNNRTG 1 cut(s) 1127
SacI GAGCTC 1 cut(s) 495
SapI GCTCTTC 1 cut(s) 671
SaqAI TTAA 4 cut(s) 855, 920, 1196, 1374
SatI GCNGC 3 cut(s) 400, 723, 895
Sau3AI GATC 3 cut(s) 762, 802, 991
Sau96I GGNCC 5 cut(s) 75, 377, 1250, 1282, 1331
ScaI AGTACT 1 cut(s) 186
SchI GAGTC 4 cut(s) 248, 422, 697, 1334
ScrFI CCNGG 7 cut(s) 97, 827, 909, 965, 1248, 1329, 1345
SduI GDGCHC 2 cut(s) 495, 989
SexAI ACCWGGT 1 cut(s) 825
SfaNI GCATC 3 cut(s) 1010, 1105, 1252
SfcI CTRYAG 3 cut(s) 27, 188, 1335
SgrAI CRCCGGYG 1 cut(s) 13
SinI GGWCC 2 cut(s) 1250, 1282
SmiMI CAYNNNNRTG 1 cut(s) 1127
Sse9I AATT 7 cut(s) 37, 116, 207, 319, 424, 564, 1151
SsiI CCGC 2 cut(s) 59, 961
SspMI CTAG 2 cut(s) 273, 929
SstI GAGCTC 1 cut(s) 495
StyD4I CCNGG 7 cut(s) 95, 825, 907, 963, 1246, 1327, 1343
TaaI ACNGT 5 cut(s) 22, 488, 886, 1081, 1126
TaqI TCGA 4 cut(s) 167, 172, 786, 1155
TasI AATT 7 cut(s) 37, 116, 207, 319, 424, 564, 1151
TatI WGTACW 1 cut(s) 184
TfiI GAWTC 6 cut(s) 164, 169, 449, 475, 501, 560
Tru1I TTAA 4 cut(s) 855, 920, 1196, 1374
Tru9I TTAA 4 cut(s) 855, 920, 1196, 1374
TscAI CASTG 3 cut(s) 355, 895, 1086
TseFI GTSAC 2 cut(s) 22, 338
TseI GCWGC 3 cut(s) 399, 722, 894
Tsp45I GTSAC 2 cut(s) 22, 338
TspDTI ATGAA 7 cut(s) 446, 541, 755, 888, 967, 1124, 1212
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 3 cut(s) 355, 895, 1086
Tth111I GACNNNGTC 1 cut(s) 884
VpaK11BI GGWCC 2 cut(s) 1250, 1282
XapI RAATTY 3 cut(s) 37, 319, 1151
XcmI CCANNNNNNNNNTGG 1 cut(s) 1040
XmiI GTMKAC 1 cut(s) 630
XmnI GAANNNNTTC 1 cut(s) 848
XspI CTAG 2 cut(s) 273, 929
ZrmI AGTACT 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.