FvH4_7g16920

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003446
Physical Location & Seq
Forward (+)
14517178 .. 14519529
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g16920.t2

Sequence Viewer

Length: 1209 bp
ATGAATTCCAATCTACCTCAGCACCAAATTGGGCAATCCCCAGCTAGGCTAGGCCTGGCAAATCCCACTTCACCATCTATTCAAGTCCCTAATCCTCCAAAACCCACTTCACAGCAATCCAATGAGTCGAAGCAATCCAATGCCTCTTCGACTCTTATCTCTCTCCTCCCACCCCTCCCAAGAGCACAGTCCCTTCTTGTCCAAATGGCCTCTCTAGCCTCCAAGCTCTTTGAAGTCTCGCCTAATCGGTCCCTCTGGCTCAATGCATTTCGTGGCTCGTTGCCAACCTTTCTGCCTTCCCAAAGCCAATCAGTGCCCATTACTGCACTTGACTCCAATCCTTCCTCCACCAAAGATATCCTGGCCCAGTTCACGGTGCTTCAAACGCAACTCTTTGAGGCTGTTGCCGAGCTCCAGGAAATTCTTGATCTTCAGGACTCTAAGCTGAAAATTGCACGTGAAATCCGGTCCAAAGATGCTGCACTTCTTGCCTTTACCAAGAAACTTAAGGATGCCGAGCAGATTCTTGATAACCTTGTTGATGACTACTCTGACTTTCGTTGCTCCAAGCGGCCAAAACAGGAGGAGGAGGATGCAGAAGATGAGGAATCTTCATGCACTACTACTGTTACTTCTAAGTTGAAATTATCGGATATTTTGTCATATGCCCACCGGATAAGCTACACAACCTTTGCACCGCCTGATTTTGGTGCCGGACAAGCTCCTCTTCGTGGGGCACTCCCACCTGCCCCACAGGATGAACAAATGCGTGCTTCTCAGCTATACCATTTTGCAAACCTTGATGTGGGATTACCTAAGACAGTTGAGAGCGAGGAGAAAGCATTTGATGCAATAATGGAGCCCCCACCTCCACAACCAACAGAATACAATCAACAAGCTGGCATGGTTCCGTTTCAGGGAATACTTCCTCCAAATTTCACAATTCCACCAGGTTGGAAACCAGGAATGCCTGTGGAATTGCCAACTGATTTACCTCCGCCCCCACCTGGATGGAAACCTGGAGATCCAGTGCCGTTGGACGCTCTCCCCATACATGTGCCCCCACAAGTACGGGCTAGTGCTCCTCAAGGAATACATAAACCACCAGCGACAATACAGGTTCAGCATGTTCAGCTTGATATTGTGGATCAAGATGATGATAGTAGTGATTATAGTAGCGATGAGGGAAGCTCCGAAGATGATGATTAA

Protein Analysis

403

Amino Acids

43.81

Weight (kDa)

4.64

Isoelectric Point (pI)

72.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med4 PF10018 120 - 269 1.3e-11 Vitamin-D-receptor interacting Mediator subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 754
Acc36I ACCTGC 1 cut(s) 754
AccB1I GGYRCC 1 cut(s) 710
AciI CCGC 3 cut(s) 571, 698, 998
AclWI GGATC 2 cut(s) 1019, 1155
AcoI YGGCCR 1 cut(s) 572
AcsI RAATTY 3 cut(s) 4, 420, 934
AcuI CTGAAG 1 cut(s) 416
AcvI CACGTG 1 cut(s) 458
AfaI GTAC 1 cut(s) 1071
AfiI CCNNNNNNNGG 7 cut(s) 45, 373, 707, 731, 805, 917, 1007
AflII CTTAAG 1 cut(s) 506
AflIII ACRYGT 1 cut(s) 1054
AgsI TTSAA 4 cut(s) 83, 233, 383, 643
AjnI CCWGG 7 cut(s) 54, 360, 414, 949, 961, 1006, 1018
AjuI GAANNNNNNNTTGG 2 cut(s) 91, 123
Alw21I GWGCWC 3 cut(s) 187, 414, 1084
Alw26I GTCTC 1 cut(s) 241
AlwI GGATC 2 cut(s) 1019, 1155
AoxI GGCC 4 cut(s) 52, 207, 363, 572
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 3 cut(s) 4, 420, 934
AspS9I GGNCC 3 cut(s) 249, 364, 468
AsuHPI GGTGA 1 cut(s) 63
AvaII GGWCC 2 cut(s) 249, 468
BaeGI GKGCMC 3 cut(s) 318, 739, 1062
BanI GGYRCC 1 cut(s) 710
BanII GRGCYC 2 cut(s) 414, 864
BbrPI CACGTG 1 cut(s) 458
Bbv12I GWGCWC 3 cut(s) 187, 414, 1084
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 466
BccI CCATC 2 cut(s) 82, 1005
BceAI ACGGC 1 cut(s) 1018
BciT130I CCWGG 7 cut(s) 56, 362, 416, 951, 963, 1008, 1020
BcoDI GTCTC 1 cut(s) 241
BfaI CTAG 4 cut(s) 45, 50, 215, 1077
BfrI CTTAAG 1 cut(s) 506
BfuAI ACCTGC 1 cut(s) 754
BisI GCNGC 2 cut(s) 480, 572
BlsI GCNGC 2 cut(s) 481, 573
Bme1390I CCNGG 7 cut(s) 56, 362, 416, 951, 963, 1008, 1020
Bme18I GGWCC 2 cut(s) 249, 468
BmgT120I GGNCC 3 cut(s) 249, 364, 468
BmiI GGNNCC 4 cut(s) 251, 712, 861, 909
BmrFI CCNGG 7 cut(s) 56, 362, 416, 951, 963, 1008, 1020
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 4 cut(s) 466, 502, 583, 838
BmuI ACTGGG 1 cut(s) 361
BplI GAGNNNNNCTC 2 cut(s) 1175, 1207
BpmI CTGGAG 2 cut(s) 398, 1041
Bpu10I CCTNAGC 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 1071
BsaAI YACGTR 1 cut(s) 458
BsaWI WCCGGW 2 cut(s) 465, 672
BsaXI ACNNNNNCTCC 2 cut(s) 1014, 1044
Bsc4I CCNNNNNNNGG 7 cut(s) 45, 373, 707, 731, 805, 917, 1007
Bse1I ACTGG 2 cut(s) 367, 1028
BseBI CCWGG 7 cut(s) 56, 362, 416, 951, 963, 1008, 1020
BseGI GGATG 4 cut(s) 517, 598, 763, 1016
BseLI CCNNNNNNNGG 7 cut(s) 45, 373, 707, 731, 805, 917, 1007
BseMII CTCAG 2 cut(s) 32, 791
BseNI ACTGG 2 cut(s) 367, 1028
BseRI GAGGAG 6 cut(s) 155, 599, 602, 714, 848, 1074
BseSI GKGCMC 3 cut(s) 318, 739, 1062
BseXI GCAGC 1 cut(s) 466
BseYI CCCAGC 1 cut(s) 40
BsgI GTGCAG 2 cut(s) 309, 465
BshFI GGCC 4 cut(s) 54, 209, 365, 574
BshNI GGYRCC 1 cut(s) 710
BsiHKAI GWGCWC 3 cut(s) 187, 414, 1084
BsiSI CCGG 3 cut(s) 466, 673, 714
BslFI GGGAC 3 cut(s) 71, 175, 235
BslI CCNNNNNNNGG 7 cut(s) 45, 373, 707, 731, 805, 917, 1007
BsmAI GTCTC 1 cut(s) 241
BsmFI GGGAC 3 cut(s) 71, 175, 235
BsmI GAATGC 1 cut(s) 972
BsnI GGCC 4 cut(s) 54, 209, 365, 574
Bsp1286I GDGCHC 7 cut(s) 187, 318, 414, 739, 864, 1062, 1084
Bsp143I GATC 3 cut(s) 427, 1024, 1147
BspACI CCGC 3 cut(s) 571, 698, 998
BspANI GGCC 4 cut(s) 54, 209, 365, 574
BspCNI CTCAG 2 cut(s) 31, 790
BspLI GGNNCC 4 cut(s) 251, 712, 861, 909
BspMI ACCTGC 1 cut(s) 754
BspPI GGATC 2 cut(s) 1019, 1155
BspT107I GGYRCC 1 cut(s) 710
BspTI CTTAAG 1 cut(s) 506
BsrI ACTGG 2 cut(s) 367, 1028
BssMI GATC 3 cut(s) 427, 1024, 1147
Bst2UI CCWGG 7 cut(s) 56, 362, 416, 951, 963, 1008, 1020
Bst4CI ACNGT 4 cut(s) 189, 376, 628, 823
Bst6I CTCTTC 2 cut(s) 151, 732
BstAFI CTTAAG 1 cut(s) 506
BstAPI GCANNNNNTGC 2 cut(s) 488, 848
BstBAI YACGTR 1 cut(s) 458
BstC8I GCNNGC 2 cut(s) 771, 901
BstDEI CTNAG 5 cut(s) 18, 441, 636, 777, 816
BstF5I GGATG 4 cut(s) 517, 598, 763, 1016
BstKTI GATC 3 cut(s) 430, 1027, 1150
BstMAI GTCTC 1 cut(s) 241
BstMBI GATC 3 cut(s) 427, 1024, 1147
BstMWI GCNNNNNNNGC 6 cut(s) 215, 385, 488, 719, 848, 1132
BstNI CCWGG 7 cut(s) 56, 362, 416, 951, 963, 1008, 1020
BstNSI RCATGY 2 cut(s) 1058, 1130
BstSCI CCNGG 7 cut(s) 54, 360, 414, 949, 961, 1006, 1018
BstSLI GKGCMC 3 cut(s) 318, 739, 1062
BstV1I GCAGC 1 cut(s) 466
BstX2I RGATCY 1 cut(s) 1024
BstXI CCANNNNNNTGG 2 cut(s) 954, 1011
BstYI RGATCY 1 cut(s) 1024
BsuRI GGCC 4 cut(s) 54, 209, 365, 574
BtgZI GCGATG 1 cut(s) 1194
BtsCI GGATG 4 cut(s) 517, 598, 763, 1016
BtsIMutI CAGTG 2 cut(s) 318, 1035
BveI ACCTGC 1 cut(s) 754
Cac8I GCNNGC 2 cut(s) 771, 901
Cfr13I GGNCC 3 cut(s) 249, 364, 468
CseI GACGC 1 cut(s) 1049
CsiI ACCWGGT 1 cut(s) 949
Csp6I GTAC 1 cut(s) 1070
CviAII CATG 4 cut(s) 615, 904, 1055, 1127
CviQI GTAC 1 cut(s) 1070
DdeI CTNAG 5 cut(s) 18, 441, 636, 777, 816
DpnI GATC 3 cut(s) 429, 1026, 1149
DpnII GATC 3 cut(s) 427, 1024, 1147
EaeI YGGCCR 1 cut(s) 572
Eam1104I CTCTTC 2 cut(s) 151, 732
EarI CTCTTC 2 cut(s) 151, 732
EciI GGCGGA 1 cut(s) 987
Ecl136II GAGCTC 1 cut(s) 412
Eco147I AGGCCT 1 cut(s) 54
Eco24I GRGCYC 2 cut(s) 414, 864
Eco32I GATATC 1 cut(s) 358
Eco47I GGWCC 2 cut(s) 249, 468
Eco53kI GAGCTC 1 cut(s) 412
Eco57I CTGAAG 1 cut(s) 416
Eco72I CACGTG 1 cut(s) 458
EcoICRI GAGCTC 1 cut(s) 412
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 7 cut(s) 54, 360, 414, 949, 961, 1006, 1018
EcoRV GATATC 1 cut(s) 358
EcoT22I ATGCAT 1 cut(s) 268
EcoT38I GRGCYC 2 cut(s) 414, 864
FaeI CATG 4 cut(s) 618, 907, 1058, 1130
FalI AAGNNNNNCTT 2 cut(s) 711, 743
FaqI GGGAC 3 cut(s) 71, 175, 235
FatI CATG 4 cut(s) 614, 903, 1054, 1126
FauNDI CATATG 1 cut(s) 664
Fnu4HI GCNGC 2 cut(s) 480, 572
FokI GGATG 4 cut(s) 524, 605, 770, 1023
FriOI GRGCYC 2 cut(s) 414, 864
Fsp4HI GCNGC 2 cut(s) 480, 572
FspBI CTAG 4 cut(s) 45, 50, 215, 1077
GluI GCNGC 2 cut(s) 480, 572
GsaI CCCAGC 1 cut(s) 44
GsuI CTGGAG 2 cut(s) 398, 1041
HaeIII GGCC 4 cut(s) 54, 209, 365, 574
HapII CCGG 3 cut(s) 466, 673, 714
HgaI GACGC 1 cut(s) 1049
Hin1II CATG 4 cut(s) 618, 907, 1058, 1130
HinfI GANTC 6 cut(s) 125, 151, 332, 437, 523, 608
HpaII CCGG 3 cut(s) 466, 673, 714
HphI GGTGA 1 cut(s) 63
Hpy166II GTNNAC 1 cut(s) 372
Hpy188I TCNGA 3 cut(s) 553, 652, 1195
Hpy188III TCNNGA 4 cut(s) 425, 434, 527, 1151
Hpy8I GTNNAC 1 cut(s) 372
HpyAV CCTTC 3 cut(s) 203, 306, 351
HpyCH4III ACNGT 4 cut(s) 189, 376, 628, 823
HpyCH4IV ACGT 1 cut(s) 457
HpyCH4V TGCA 9 cut(s) 266, 326, 455, 482, 596, 618, 695, 794, 851
HpyF10VI GCNNNNNNNGC 6 cut(s) 215, 385, 488, 719, 848, 1132
HpyF3I CTNAG 5 cut(s) 18, 441, 636, 777, 816
HpySE526I ACGT 1 cut(s) 457
Hsp92II CATG 4 cut(s) 618, 907, 1058, 1130
Kzo9I GATC 3 cut(s) 427, 1024, 1147
LmnI GCTCC 6 cut(s) 417, 569, 727, 859, 1087, 1196
Lsp1109I GCAGC 1 cut(s) 466
LweI GCATC 4 cut(s) 466, 502, 583, 838
MabI ACCWGGT 1 cut(s) 949
MaeI CTAG 4 cut(s) 45, 50, 215, 1077
MaeII ACGT 1 cut(s) 457
MaeIII GTNAC 1 cut(s) 628
MalI GATC 3 cut(s) 429, 1026, 1149
MboI GATC 3 cut(s) 427, 1024, 1147
MboII GAAGA 6 cut(s) 138, 422, 603, 611, 719, 1208
MflI RGATCY 1 cut(s) 1024
MhlI GDGCHC 7 cut(s) 187, 318, 414, 739, 864, 1062, 1084
MluCI AATT 8 cut(s) 4, 27, 420, 450, 644, 934, 942, 977
MlyI GAGTC 4 cut(s) 134, 145, 326, 431
MmeI TCCRAC 2 cut(s) 935, 1017
Mph1103I ATGCAT 1 cut(s) 268
MseI TTAA 2 cut(s) 507, 1207
MslI CAYNNNNRTG 2 cut(s) 1009, 1055
MspCI CTTAAG 1 cut(s) 506
MspI CCGG 3 cut(s) 466, 673, 714
MspR9I CCNGG 7 cut(s) 56, 362, 416, 951, 963, 1008, 1020
Mva1269I GAATGC 1 cut(s) 972
MvaI CCWGG 7 cut(s) 56, 362, 416, 951, 963, 1008, 1020
MwoI GCNNNNNNNGC 6 cut(s) 215, 385, 488, 719, 848, 1132
NdeI CATATG 1 cut(s) 664
NdeII GATC 3 cut(s) 427, 1024, 1147
NlaIII CATG 4 cut(s) 618, 907, 1058, 1130
NlaIV GGNNCC 4 cut(s) 251, 712, 861, 909
NmeAIII GCCGAG 2 cut(s) 433, 541
NsiI ATGCAT 1 cut(s) 268
NspI RCATGY 2 cut(s) 1058, 1130
PaqCI CACCTGC 1 cut(s) 754
PceI AGGCCT 1 cut(s) 54
PciI ACATGT 1 cut(s) 1054
PctI GAATGC 1 cut(s) 972
PfeI GAWTC 2 cut(s) 523, 608
PfoI TCCNGGA 1 cut(s) 414
PkrI GCNGC 2 cut(s) 481, 573
PleI GAGTC 4 cut(s) 133, 145, 326, 431
PmaCI CACGTG 1 cut(s) 458
PmlI CACGTG 1 cut(s) 458
PpsI GAGTC 4 cut(s) 133, 145, 326, 431
Ppu21I YACGTR 1 cut(s) 458
PscI ACATGT 1 cut(s) 1054
Psp124BI GAGCTC 1 cut(s) 414
Psp6I CCWGG 7 cut(s) 54, 360, 414, 949, 961, 1006, 1018
PspCI CACGTG 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 40
PspGI CCWGG 7 cut(s) 54, 360, 414, 949, 961, 1006, 1018
PspN4I GGNNCC 4 cut(s) 251, 712, 861, 909
PspPI GGNCC 3 cut(s) 249, 364, 468
PsuI RGATCY 1 cut(s) 1024
RsaI GTAC 1 cut(s) 1071
RsaNI GTAC 1 cut(s) 1070
RseI CAYNNNNRTG 2 cut(s) 1009, 1055
SacI GAGCTC 1 cut(s) 414
SaqAI TTAA 2 cut(s) 507, 1207
SatI GCNGC 2 cut(s) 480, 572
Sau3AI GATC 3 cut(s) 427, 1024, 1147
Sau96I GGNCC 3 cut(s) 249, 364, 468
SchI GAGTC 4 cut(s) 134, 145, 326, 431
ScrFI CCNGG 7 cut(s) 56, 362, 416, 951, 963, 1008, 1020
SduI GDGCHC 7 cut(s) 187, 318, 414, 739, 864, 1062, 1084
SexAI ACCWGGT 1 cut(s) 949
SfaNI GCATC 4 cut(s) 466, 502, 583, 838
SinI GGWCC 2 cut(s) 249, 468
SmiMI CAYNNNNRTG 2 cut(s) 1009, 1055
SmlI CTYRAG 2 cut(s) 506, 1086
SmoI CTYRAG 2 cut(s) 506, 1086
Sse9I AATT 8 cut(s) 4, 27, 420, 450, 644, 934, 942, 977
SseBI AGGCCT 1 cut(s) 54
SsiI CCGC 3 cut(s) 571, 698, 998
SspMI CTAG 4 cut(s) 45, 50, 215, 1077
SstI GAGCTC 1 cut(s) 414
StuI AGGCCT 1 cut(s) 54
StyD4I CCNGG 7 cut(s) 54, 360, 414, 949, 961, 1006, 1018
TaaI ACNGT 4 cut(s) 189, 376, 628, 823
TaiI ACGT 1 cut(s) 460
TaqI TCGA 2 cut(s) 128, 149
TaqII GACCGA 1 cut(s) 237
TasI AATT 8 cut(s) 4, 27, 420, 450, 644, 934, 942, 977
TauI GCSGC 1 cut(s) 574
TfiI GAWTC 2 cut(s) 523, 608
Tru1I TTAA 2 cut(s) 507, 1207
Tru9I TTAA 2 cut(s) 507, 1207
TscAI CASTG 2 cut(s) 318, 1035
TseI GCWGC 1 cut(s) 479
TspDTI ATGAA 3 cut(s) 17, 603, 774
TspGWI ACGGA 1 cut(s) 900
TspRI CASTG 2 cut(s) 318, 1035
Vha464I CTTAAG 1 cut(s) 506
VpaK11BI GGWCC 2 cut(s) 249, 468
XapI RAATTY 3 cut(s) 4, 420, 934
XceI RCATGY 2 cut(s) 1058, 1130
XcmI CCANNNNNNNNNTGG 1 cut(s) 358
XspI CTAG 4 cut(s) 45, 50, 215, 1077
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.