pycom01g11270

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003446
Physical Location & Seq
Reverse (-)
12242370 .. 12243560
1191 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g11270.1

Sequence Viewer

Length: 1191 bp
ATGCTGCAATCCCAGATTGTTCAATCCCCCGCTAGGCTAGGCCTAACAAACCCCACATCGCCCTCTCTTCAGAACCCTACGCCTCCAAAAGTCCCTTCACAGCAGCAACCCAATCTCTCCCCAACCCTTATCTCTCTCCTTCCACCGCTCCCTAGGGCTCAATCCATTCTTGTCCAAATGGCCTCCCTTGCCTCTAAACTCTTTGAAGTCTCTCCCAATCGGTCACTTTGGCTCAATGCATTTCGGGGGACCTTCCCGACTTTTCTTTCTTCCCAAACTCAGTCACTTCCCTCAACTGCACTTGAATCTTCTCCTTCCTCCACCAAAGAGATCCTCTCCCAGTTCACTGCCCTTCAAACCCAACTCTTTGAGGCTGTTGCTGAACTCCAAGAAATTCTTGATCTTCAGGACGCCAAGCTGAAAATTGCACGTGAAGTGAAGGCCAAGGATGCTGCGCTTCTTTCCTTTTCCAAGAAACTCAGGGATGTTGAGCAGGTTCTTGATAATCTTGTTGATGATTACTCTGATTTTACCCGCCCCAAACGGACAAAACAGGAGGACGGTGCAGATGATGATTCTTCATGCACCACCACTACGGTTTCATCTCAGTTGAATTTGTCAGATATACTGTCGTATGCCCACCGGATAAGCTACACAACCTTTGCACCACCAGAGTTTGGGGCGGGACAAGCTCCTCTTCGTGGGGCACTCCCACCTGCTCCACAGGACGAGCAAATGCGTGCTTCTCAGCTGTATGCTTTTGCAAACCTTGATGTGGGCCTACCTAAAACAGTCGAGGGGAAGGAGGAAACGATGCAGGCAATATTGGAGCCCCCGCCTGCTGAACAAACAGAATCTAATCAACTTCCCAACCTGGGTGCACTCCAAGGTTTGCTTCCTCCCAACATCACAGTCCCGTCAGGTTGGAAACCAGGGATGCCTGTGGAATTGCCAACCGAATTTCCTGTGCCCCCACCTGGATGGAAGCCAGGGGACCCAGTGCCTCTTCCCCCATTTGAATCTCTTCCAGTTCCTAGGGCCGAGGAGCAACAAATACGACCTAATGCTCATCCAGGAGTACATAAACCACCAGCGACAATACAGGTTCAGCATGTTGAGCTGGATATTCTGGATCCAGACGATGATAGCGATTATAGTACTGAAGATGGAAGCTCAGACGATGATGAGTGA

Protein Analysis

397

Amino Acids

43.07

Weight (kDa)

4.54

Isoelectric Point (pI)

74.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med4 PF10018 110 - 284 7.5e-12 Vitamin-D-receptor interacting Mediator subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 724
Acc36I ACCTGC 2 cut(s) 484, 724
AccB7I CCANNNNNTGG 1 cut(s) 677
AccBSI CCGCTC 1 cut(s) 148
AciI CCGC 5 cut(s) 30, 146, 535, 683, 836
AclWI GGATC 3 cut(s) 325, 1127, 1140
AcsI RAATTY 3 cut(s) 393, 613, 959
AcuI CTGAAG 3 cut(s) 53, 389, 1182
AcvI CACGTG 1 cut(s) 431
AcyI GRCGYC 1 cut(s) 411
AfaI GTAC 2 cut(s) 1080, 1159
AfiI CCNNNNNNNGG 6 cut(s) 33, 677, 701, 775, 875, 977
AgsI TTSAA 6 cut(s) 23, 206, 305, 356, 613, 1019
AjnI CCWGG 5 cut(s) 873, 931, 976, 988, 1072
AjuI GAANNNNNNNTTGG 4 cut(s) 79, 111, 879, 911
AluBI AGCT 6 cut(s) 418, 651, 692, 751, 1120, 1173
AluI AGCT 6 cut(s) 418, 651, 692, 751, 1120, 1173
Alw21I GWGCWC 1 cut(s) 883
Alw26I GTCTC 1 cut(s) 214
Alw44I GTGCAC 1 cut(s) 879
AlwI GGATC 3 cut(s) 325, 1127, 1140
AoxI GGCC 5 cut(s) 40, 180, 441, 778, 1038
ApaLI GTGCAC 1 cut(s) 879
ApeKI GCWGC 3 cut(s) 4, 103, 452
ApoI RAATTY 3 cut(s) 393, 613, 959
Asp700I GAANNNNTTC 1 cut(s) 1023
AspA2I CCTAGG 2 cut(s) 152, 1034
AspLEI GCGC 1 cut(s) 457
AspS9I GGNCC 4 cut(s) 249, 778, 994, 1038
AvaII GGWCC 2 cut(s) 249, 994
AvrII CCTAGG 2 cut(s) 152, 1034
BaeGI GKGCMC 3 cut(s) 709, 883, 972
BamHI GGATCC 1 cut(s) 1132
BanII GRGCYC 2 cut(s) 160, 834
BbrPI CACGTG 1 cut(s) 431
Bbv12I GWGCWC 1 cut(s) 883
BbvI GCAGC 2 cut(s) 115, 439
BccI CCATC 2 cut(s) 975, 1160
BciT130I CCWGG 5 cut(s) 875, 933, 978, 990, 1074
BcoDI GTCTC 1 cut(s) 214
BfaI CTAG 4 cut(s) 33, 38, 153, 1035
BfuAI ACCTGC 2 cut(s) 484, 724
BisI GCNGC 3 cut(s) 5, 104, 453
BlnI CCTAGG 2 cut(s) 152, 1034
BlsI GCNGC 3 cut(s) 6, 105, 454
BmcAI AGTACT 1 cut(s) 1159
Bme1390I CCNGG 5 cut(s) 875, 933, 978, 990, 1074
Bme18I GGWCC 2 cut(s) 249, 994
BmgT120I GGNCC 4 cut(s) 249, 778, 994, 1038
BmiI GGNNCC 5 cut(s) 250, 831, 995, 996, 1134
BmrFI CCNGG 5 cut(s) 875, 933, 978, 990, 1074
BmrI ACTGGG 2 cut(s) 334, 992
BmsI GCATC 3 cut(s) 439, 804, 927
BmuI ACTGGG 2 cut(s) 334, 992
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BsaAI YACGTR 1 cut(s) 431
BsaHI GRCGYC 1 cut(s) 411
BsaJI CCNNGG 8 cut(s) 152, 444, 874, 886, 932, 989, 1034, 1041
BsaWI WCCGGW 1 cut(s) 642
Bsc4I CCNNNNNNNGG 6 cut(s) 33, 677, 701, 775, 875, 977
Bse1I ACTGG 3 cut(s) 340, 998, 1028
BseBI CCWGG 5 cut(s) 875, 933, 978, 990, 1074
BseDI CCNNGG 8 cut(s) 152, 444, 874, 886, 932, 989, 1034, 1041
BseGI GGATG 5 cut(s) 454, 490, 942, 986, 1069
BseLI CCNNNNNNNGG 6 cut(s) 33, 677, 701, 775, 875, 977
BseMII CTCAG 5 cut(s) 293, 493, 620, 761, 1188
BseNI ACTGG 3 cut(s) 340, 998, 1028
BseRI GAGGAG 2 cut(s) 684, 1058
BseSI GKGCMC 3 cut(s) 709, 883, 972
BseXI GCAGC 2 cut(s) 115, 439
BsgI GTGCAG 2 cut(s) 282, 585
BshFI GGCC 5 cut(s) 42, 182, 443, 780, 1040
BsiHKAI GWGCWC 1 cut(s) 883
BsiSI CCGG 1 cut(s) 643
BslFI GGGAC 5 cut(s) 77, 262, 699, 899, 1007
BslI CCNNNNNNNGG 6 cut(s) 33, 677, 701, 775, 875, 977
BsmAI GTCTC 1 cut(s) 214
BsmFI GGGAC 5 cut(s) 77, 262, 699, 899, 1007
BsnI GGCC 5 cut(s) 42, 182, 443, 780, 1040
Bsp1286I GDGCHC 5 cut(s) 160, 709, 834, 883, 972
Bsp143I GATC 3 cut(s) 330, 400, 1132
BspACI CCGC 5 cut(s) 30, 146, 535, 683, 836
BspANI GGCC 5 cut(s) 42, 182, 443, 780, 1040
BspCNI CTCAG 5 cut(s) 292, 492, 619, 760, 1187
BspLI GGNNCC 5 cut(s) 250, 831, 995, 996, 1134
BspMI ACCTGC 2 cut(s) 484, 724
BspPI GGATC 3 cut(s) 325, 1127, 1140
BsrBI CCGCTC 1 cut(s) 148
BsrI ACTGG 3 cut(s) 340, 998, 1028
BssECI CCNNGG 8 cut(s) 152, 444, 874, 886, 932, 989, 1034, 1041
BssMI GATC 3 cut(s) 330, 400, 1132
BssNI GRCGYC 1 cut(s) 411
BssT1I CCWWGG 4 cut(s) 152, 444, 886, 1034
Bst2UI CCWGG 5 cut(s) 875, 933, 978, 990, 1074
Bst4CI ACNGT 5 cut(s) 563, 598, 630, 793, 913
Bst6I CTCTTC 4 cut(s) 72, 702, 1011, 1029
BstACI GRCGYC 1 cut(s) 411
BstBAI YACGTR 1 cut(s) 431
BstC8I GCNNGC 3 cut(s) 741, 819, 840
BstDEI CTNAG 5 cut(s) 279, 479, 606, 747, 1174
BstF5I GGATG 5 cut(s) 454, 490, 942, 986, 1069
BstHHI GCGC 1 cut(s) 457
BstKTI GATC 3 cut(s) 333, 403, 1135
BstMAI GTCTC 1 cut(s) 214
BstMBI GATC 3 cut(s) 330, 400, 1132
BstMWI GCNNNNNNNGC 4 cut(s) 188, 449, 689, 1117
BstNI CCWGG 5 cut(s) 875, 933, 978, 990, 1074
BstNSI RCATGY 1 cut(s) 1115
BstSCI CCNGG 5 cut(s) 873, 931, 976, 988, 1072
BstSLI GKGCMC 3 cut(s) 709, 883, 972
BstV1I GCAGC 2 cut(s) 115, 439
BstX2I RGATCY 2 cut(s) 330, 1132
BstXI CCANNNNNNTGG 1 cut(s) 981
BstYI RGATCY 2 cut(s) 330, 1132
BsuRI GGCC 5 cut(s) 42, 182, 443, 780, 1040
BtgZI GCGATG 1 cut(s) 42
BtsCI GGATG 5 cut(s) 454, 490, 942, 986, 1069
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 2 cut(s) 345, 1005
BveI ACCTGC 2 cut(s) 484, 724
Cac8I GCNNGC 3 cut(s) 741, 819, 840
CfoI GCGC 1 cut(s) 457
Cfr13I GGNCC 4 cut(s) 249, 778, 994, 1038
CseI GACGC 1 cut(s) 419
Csp6I GTAC 2 cut(s) 1079, 1158
CviAII CATG 2 cut(s) 582, 1112
CviQI GTAC 2 cut(s) 1079, 1158
DdeI CTNAG 5 cut(s) 279, 479, 606, 747, 1174
DpnI GATC 3 cut(s) 332, 402, 1134
DpnII GATC 3 cut(s) 330, 400, 1132
Eam1104I CTCTTC 4 cut(s) 72, 702, 1011, 1029
EarI CTCTTC 4 cut(s) 72, 702, 1011, 1029
Eco130I CCWWGG 4 cut(s) 152, 444, 886, 1034
Eco147I AGGCCT 1 cut(s) 42
Eco24I GRGCYC 2 cut(s) 160, 834
Eco47I GGWCC 2 cut(s) 249, 994
Eco57I CTGAAG 3 cut(s) 53, 389, 1182
Eco72I CACGTG 1 cut(s) 431
EcoO109I RGGNCCY 2 cut(s) 249, 994
EcoRII CCWGG 5 cut(s) 873, 931, 976, 988, 1072
EcoT14I CCWWGG 4 cut(s) 152, 444, 886, 1034
EcoT22I ATGCAT 1 cut(s) 241
EcoT38I GRGCYC 2 cut(s) 160, 834
ErhI CCWWGG 4 cut(s) 152, 444, 886, 1034
FaeI CATG 2 cut(s) 585, 1115
FaiI YATR 7 cut(s) 583, 626, 636, 756, 1083, 1113, 1155
FalI AAGNNNNNCTT 6 cut(s) 381, 413, 681, 713, 879, 911
FaqI GGGAC 5 cut(s) 77, 262, 699, 899, 1007
FatI CATG 2 cut(s) 581, 1111
FauI CCCGC 4 cut(s) 37, 542, 676, 843
Fnu4HI GCNGC 3 cut(s) 5, 104, 453
FokI GGATG 5 cut(s) 461, 497, 949, 993, 1056
FriOI GRGCYC 2 cut(s) 160, 834
Fsp4HI GCNGC 3 cut(s) 5, 104, 453
FspBI CTAG 4 cut(s) 33, 38, 153, 1035
GlaI GCGC 1 cut(s) 456
GluI GCNGC 3 cut(s) 5, 104, 453
HaeIII GGCC 5 cut(s) 42, 182, 443, 780, 1040
HapII CCGG 1 cut(s) 643
HgaI GACGC 1 cut(s) 419
HhaI GCGC 1 cut(s) 457
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 2 cut(s) 585, 1115
Hin6I GCGC 1 cut(s) 455
HinP1I GCGC 1 cut(s) 455
HinfI GANTC 4 cut(s) 305, 575, 854, 1019
HpaII CCGG 1 cut(s) 643
Hpy166II GTNNAC 2 cut(s) 345, 881
Hpy188I TCNGA 4 cut(s) 72, 526, 622, 1177
Hpy188III TCNNGA 6 cut(s) 256, 398, 407, 500, 1130, 1136
Hpy8I GTNNAC 2 cut(s) 345, 881
HpyAV CCTTC 7 cut(s) 105, 149, 262, 324, 362, 433, 796
HpyCH4III ACNGT 5 cut(s) 563, 598, 630, 793, 913
HpyCH4IV ACGT 1 cut(s) 430
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 449, 689, 1117
HpyF3I CTNAG 5 cut(s) 279, 479, 606, 747, 1174
HpySE526I ACGT 1 cut(s) 430
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 2 cut(s) 585, 1115
HspAI GCGC 1 cut(s) 455
KflI GGGWCCC 1 cut(s) 994
Kzo9I GATC 3 cut(s) 330, 400, 1132
LmnI GCTCC 5 cut(s) 153, 697, 724, 829, 1045
Lsp1109I GCAGC 2 cut(s) 115, 439
LweI GCATC 3 cut(s) 439, 804, 927
MaeI CTAG 4 cut(s) 33, 38, 153, 1035
MaeII ACGT 1 cut(s) 430
MaeIII GTNAC 2 cut(s) 222, 282
MalI GATC 3 cut(s) 332, 402, 1134
MbiI CCGCTC 1 cut(s) 148
MboI GATC 3 cut(s) 330, 400, 1132
MboII GAAGA 9 cut(s) 59, 261, 300, 395, 570, 689, 998, 1016, 1175
MflI RGATCY 2 cut(s) 330, 1132
MhlI GDGCHC 5 cut(s) 160, 709, 834, 883, 972
MluCI AATT 5 cut(s) 393, 423, 613, 947, 959
MmeI TCCRAC 1 cut(s) 905
Mph1103I ATGCAT 1 cut(s) 241
MroXI GAANNNNTTC 1 cut(s) 1023
MslI CAYNNNNRTG 1 cut(s) 979
MspA1I CMGCKG 1 cut(s) 751
MspI CCGG 1 cut(s) 643
MspR9I CCNGG 5 cut(s) 875, 933, 978, 990, 1074
MvaI CCWGG 5 cut(s) 875, 933, 978, 990, 1074
MwoI GCNNNNNNNGC 4 cut(s) 188, 449, 689, 1117
NdeII GATC 3 cut(s) 330, 400, 1132
NlaIII CATG 2 cut(s) 585, 1115
NlaIV GGNNCC 5 cut(s) 250, 831, 995, 996, 1134
NmeAIII GCCGAG 1 cut(s) 1066
NmuCI GTSAC 2 cut(s) 222, 282
NsiI ATGCAT 1 cut(s) 241
NspI RCATGY 1 cut(s) 1115
PaqCI CACCTGC 1 cut(s) 724
PceI AGGCCT 1 cut(s) 42
PcsI WCGNNNNNNNCGW 1 cut(s) 1146
PdmI GAANNNNTTC 1 cut(s) 1023
PfeI GAWTC 4 cut(s) 305, 575, 854, 1019
PflMI CCANNNNNTGG 1 cut(s) 677
PfoI TCCNGGA 1 cut(s) 1072
PkrI GCNGC 3 cut(s) 6, 105, 454
PmaCI CACGTG 1 cut(s) 431
PmlI CACGTG 1 cut(s) 431
Ppu21I YACGTR 1 cut(s) 431
PpuMI RGGWCCY 2 cut(s) 249, 994
Psp5II RGGWCCY 2 cut(s) 249, 994
Psp6I CCWGG 5 cut(s) 873, 931, 976, 988, 1072
PspCI CACGTG 1 cut(s) 431
PspGI CCWGG 5 cut(s) 873, 931, 976, 988, 1072
PspN4I GGNNCC 5 cut(s) 250, 831, 995, 996, 1134
PspPI GGNCC 4 cut(s) 249, 778, 994, 1038
PspPPI RGGWCCY 2 cut(s) 249, 994
PsuI RGATCY 2 cut(s) 330, 1132
PvuII CAGCTG 1 cut(s) 751
RsaI GTAC 2 cut(s) 1080, 1159
RsaNI GTAC 2 cut(s) 1079, 1158
RseI CAYNNNNRTG 1 cut(s) 979
SatI GCNGC 3 cut(s) 5, 104, 453
Sau3AI GATC 3 cut(s) 330, 400, 1132
Sau96I GGNCC 4 cut(s) 249, 778, 994, 1038
ScaI AGTACT 1 cut(s) 1159
ScrFI CCNGG 5 cut(s) 875, 933, 978, 990, 1074
SduI GDGCHC 5 cut(s) 160, 709, 834, 883, 972
SfaNI GCATC 3 cut(s) 439, 804, 927
SinI GGWCC 2 cut(s) 249, 994
SmiMI CAYNNNNRTG 1 cut(s) 979
Sse9I AATT 5 cut(s) 393, 423, 613, 947, 959
SseBI AGGCCT 1 cut(s) 42
SsiI CCGC 5 cut(s) 30, 146, 535, 683, 836
SspI AATATT 1 cut(s) 825
SspMI CTAG 4 cut(s) 33, 38, 153, 1035
StuI AGGCCT 1 cut(s) 42
StyD4I CCNGG 5 cut(s) 873, 931, 976, 988, 1072
StyI CCWWGG 4 cut(s) 152, 444, 886, 1034
TaaI ACNGT 5 cut(s) 563, 598, 630, 793, 913
TaiI ACGT 1 cut(s) 433
TaqI TCGA 1 cut(s) 795
TaqII GACCGA 1 cut(s) 210
TasI AATT 5 cut(s) 393, 423, 613, 947, 959
TatI WGTACW 2 cut(s) 1078, 1157
TfiI GAWTC 4 cut(s) 305, 575, 854, 1019
TscAI CASTG 2 cut(s) 352, 1005
TseFI GTSAC 2 cut(s) 222, 282
TseI GCWGC 3 cut(s) 4, 103, 452
Tsp45I GTSAC 2 cut(s) 222, 282
TspDTI ATGAA 2 cut(s) 570, 591
TspGWI ACGGA 1 cut(s) 559
TspRI CASTG 2 cut(s) 352, 1005
Van91I CCANNNNNTGG 1 cut(s) 677
VneI GTGCAC 1 cut(s) 879
VpaK11BI GGWCC 2 cut(s) 249, 994
XapI RAATTY 3 cut(s) 393, 613, 959
XceI RCATGY 1 cut(s) 1115
XmaJI CCTAGG 2 cut(s) 152, 1034
XmnI GAANNNNTTC 1 cut(s) 1023
XspI CTAG 4 cut(s) 33, 38, 153, 1035
ZrmI AGTACT 1 cut(s) 1159
Zsp2I ATGCAT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.