Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0012520

Physical Location & Seq

Genomic Coordinates Forward (+)

15963477 .. 15966697

Gene Structure 3221 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g19340.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1035 bp

ATGATCACACCGAGCTTCGTCGACGAGATTGACTGCGGCAGCTTCTTCGACAACATCGACGACCTCATGGAGGACGTCGACTCCGTCCTCGGCCCCGCTCCTGACTGTAACTCCGCCTTCTCCCTCTGGCCCGCCGCCTCCTCCGCCGCCGCCGCTCCACTCCCACCTCCCGACGCCGCCGTCTTCTCCGGCAACGGTGACTCCGACTTCTCCTCCGAGCTCCCCGTTCCGTATGAAGAGATGGTGCATTTGGAATGGCTGTCCAACTTTGTTGAGAATTCCGAATACTCTGGCGGAGACCTCACCATGAACAAGCAACAGGAGTCTTACTCCTTGGTCAACAAGGAGTCTTCCCACCAACAGTTCCAGACCTCCAGCCCCATTTCTGTGCTTGACAGCAGCAGCTCCTGCTCGGGCGAGAAGAATGTGCTTGAAGGATCAATTGCTCCTGGCAGGCGGGGACGTGCTAGAAGCAAGCGTCCTCGCCCTGCTACTTTCAATCCTCGCTCTGCAATTCAACTGATTTCCCCTGCTTCCTCTGTCTCGGAGATTGACAACCCTCTTCAGCAGCATTCATTGTTTGCTGCTCCCAAGGTGTTTTCGGAGTCTGAGAACTTTGCTGAGTCTCGTCCTGTGGTGAAGATGCCGAAACTGGCTTCTGGGGAGCAGAAGAAGAAGAAAAGAATCAAGTTGCCCCCTCAGGTTCCAGGGGAGCTGGACGAGAACTCTCCATTGCAGGGAGCAATTAGGAAGTGCTTGCATTGTGAGATTACCAAAACACCCCAGTGGAGGGCAGGCCCAATGGGGCCAAAAACCCTATGCAATGCCTGTGGAGTGCGCTACAAGTCAGGCAGGCTCTTCCCTGAGTACCGTCCTGCAGCTAGTCCAACTTTTGTTCCAGCCTTGCACTCCAATTCCCATAAGAAGGTTCTCGAGATGAGAACCAAGGGTGGCGAGATGGTTTCTGTTCACGACAACAGCCTCCCAGAACTCATTCCGAATACTAACAGCAGCATTTCGCTGGATTACATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000976 GO:0000977 GO:0000981 GO:0001012 GO:0001067 GO:0001085 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009628 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048511 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090351 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GATA

Protein Analysis

345

Amino Acids

37.44

Weight (kDa)

5.59

Isoelectric Point (pI)

62.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATA	PF00320	172 - 205	1.5e-14	GATA zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012520)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54810 AT3G54810
fragaria_vesca	FvH4_7g19340 FvH4_7g19340 FvH4_7g19340
malus_domestica	MD01G1108000.v1.1 MD07G1173900.v1.1
prunus_persica	Prupe.2G212900_v2.0.a1 Prupe.2G212900_v2.0.a1
pyrus_communis	pycom01g13490 pycom07g16830
rosa_chinensis	RchiOBHm_Chr1g0363221
rosa_laevigata	RLG00000027582
rosa_multiflora	Rmu_sc0001770.1_g000004
rosa_roxburghii	Rroxscaffold_4G00293350
rosa_samantha	Rh1AG314400 Rh1CG294300 Rh1DG308700
rosa_wichuraiana	Rw1G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	179
AatII	GACGTC	1 cut(s)	78
AccBSI	CCGCTC	2 cut(s)	98, 155
AccI	GTMKAC	2 cut(s)	21, 78
AclWI	GGATC	1 cut(s)	445
AcsI	RAATTY	1 cut(s)	277
AcuI	CTGAAG	1 cut(s)	548
AcyI	GRCGYC	2 cut(s)	75, 174
AfaI	GTAC	1 cut(s)	869
AfiI	CCNNNNNNNGG	5 cut(s)	70, 737, 789, 790, 925
AflIII	ACRYGT	1 cut(s)	1029
AgsI	TTSAA	3 cut(s)	434, 499, 518
AjiI	CACGTC	1 cut(s)	464
AjnI	CCWGG	2 cut(s)	448, 706
AleI	CACNNNNGTG	1 cut(s)	784
AluBI	AGCT	6 cut(s)	15, 42, 220, 405, 715, 881
AluI	AGCT	6 cut(s)	15, 42, 220, 405, 715, 881
Alw21I	GWGCWC	1 cut(s)	222
Alw26I	GTCTC	3 cut(s)	291, 547, 630
AlwI	GGATC	1 cut(s)	445
AlwNI	CAGNNNCTG	1 cut(s)	408
Ama87I	CYCGRG	2 cut(s)	412, 932
AoxI	GGCC	4 cut(s)	91, 128, 796, 806
ApeKI	GCWGC	7 cut(s)	39, 399, 402, 568, 584, 878, 1011
ApoI	RAATTY	1 cut(s)	277
Asp700I	GAANNNNTTC	1 cut(s)	993
AspLEI	GCGC	1 cut(s)	840
AspS9I	GGNCC	4 cut(s)	92, 129, 797, 806
AsuHPI	GGTGA	3 cut(s)	209, 295, 649
AvaI	CYCGRG	2 cut(s)	412, 932
AxyI	CCTNAGG	1 cut(s)	699
BanII	GRGCYC	1 cut(s)	222
BbsI	GAAGAC	2 cut(s)	175, 342
Bbv12I	GWGCWC	1 cut(s)	222
BbvI	GCAGC	7 cut(s)	51, 411, 414, 571, 580, 890, 1023
BccI	CCATC	2 cut(s)	235, 952
BceAI	ACGGC	1 cut(s)	164
BcgI	CGANNNNNNTGC	2 cut(s)	28, 62
BciT130I	CCWGG	2 cut(s)	450, 708
BclI	TGATCA	1 cut(s)	3
BcoDI	GTCTC	3 cut(s)	291, 547, 630
BfaI	CTAG	2 cut(s)	468, 882
BfmI	CTRYAG	1 cut(s)	876
Bme1390I	CCNGG	2 cut(s)	450, 708
BmeT110I	CYCGRG	2 cut(s)	412, 932
BmgBI	CACGTC	1 cut(s)	464
BmgT120I	GGNCC	4 cut(s)	92, 129, 797, 806
BmiI	GGNNCC	3 cut(s)	94, 705, 807
BmrFI	CCNGG	2 cut(s)	450, 708
BmrI	ACTGGG	1 cut(s)	778
BmsI	GCATC	1 cut(s)	633
BmuI	ACTGGG	1 cut(s)	778
BpiI	GAAGAC	2 cut(s)	175, 342
BplI	GAGNNNNNCTC	2 cut(s)	314, 346
BpmI	CTGGAG	1 cut(s)	358
BsaHI	GRCGYC	2 cut(s)	75, 174
BsaI	GGTCTC	1 cut(s)	291
BsaJI	CCNNGG	5 cut(s)	88, 333, 591, 707, 945
BsaXI	ACNNNNNCTCC	8 cut(s)	65, 95, 95, 125, 185, 197, 215, 227
Bsc4I	CCNNNNNNNGG	5 cut(s)	70, 737, 789, 790, 925
Bse1I	ACTGG	2 cut(s)	657, 784
Bse21I	CCTNAGG	1 cut(s)	699
Bse3DI	GCAATG	2 cut(s)	731, 829
BseBI	CCWGG	2 cut(s)	450, 708
BseDI	CCNNGG	5 cut(s)	88, 333, 591, 707, 945
BseLI	CCNNNNNNNGG	5 cut(s)	70, 737, 789, 790, 925
BseMI	GCAATG	2 cut(s)	731, 829
BseMII	CTCAG	4 cut(s)	600, 612, 713, 855
BseNI	ACTGG	2 cut(s)	657, 784
BseRI	GAGGAG	2 cut(s)	130, 202
BseXI	GCAGC	7 cut(s)	51, 411, 414, 571, 580, 890, 1023
BshFI	GGCC	4 cut(s)	93, 130, 798, 808
BsiHKAI	GWGCWC	1 cut(s)	222
BsiHKCI	CYCGRG	2 cut(s)	412, 932
BsiSI	CCGG	1 cut(s)	189
BslFI	GGGAC	1 cut(s)	474
BslI	CCNNNNNNNGG	5 cut(s)	70, 737, 789, 790, 925
BsmAI	GTCTC	3 cut(s)	291, 547, 630
BsmFI	GGGAC	1 cut(s)	474
BsmI	GAATGC	1 cut(s)	571
BsnI	GGCC	4 cut(s)	93, 130, 798, 808
Bso31I	GGTCTC	1 cut(s)	291
BsoBI	CYCGRG	2 cut(s)	412, 932
Bsp1286I	GDGCHC	1 cut(s)	222
Bsp143I	GATC	2 cut(s)	3, 437
BspANI	GGCC	4 cut(s)	93, 130, 798, 808
BspCNI	CTCAG	4 cut(s)	601, 613, 712, 856
BspLI	GGNNCC	3 cut(s)	94, 705, 807
BspMAI	CTGCAG	1 cut(s)	880
BspPI	GGATC	1 cut(s)	445
BspQI	GCTCTTC	1 cut(s)	863
BspTNI	GGTCTC	1 cut(s)	291
BsrBI	CCGCTC	2 cut(s)	98, 155
BsrDI	GCAATG	2 cut(s)	731, 829
BsrI	ACTGG	2 cut(s)	657, 784
BssECI	CCNNGG	5 cut(s)	88, 333, 591, 707, 945
BssMI	GATC	2 cut(s)	3, 437
BssNI	GRCGYC	2 cut(s)	75, 174
BssT1I	CCWWGG	3 cut(s)	333, 591, 945
Bst2UI	CCWGG	2 cut(s)	450, 708
Bst4CI	ACNGT	4 cut(s)	107, 197, 363, 872
Bst6I	CTCTTC	3 cut(s)	231, 567, 863
BstACI	GRCGYC	2 cut(s)	75, 174
BstAPI	GCANNNNNTGC	1 cut(s)	408
BstC8I	GCNNGC	6 cut(s)	132, 455, 476, 758, 796, 854
BstDEI	CTNAG	4 cut(s)	609, 621, 699, 864
BstENI	CCTNNNNNAGG	1 cut(s)	68
BstHHI	GCGC	1 cut(s)	840
BstKTI	GATC	2 cut(s)	6, 440
BstMAI	GTCTC	3 cut(s)	291, 547, 630
BstMBI	GATC	2 cut(s)	3, 437
BstMWI	GCNNNNNNNGC	3 cut(s)	143, 152, 408
BstNI	CCWGG	2 cut(s)	450, 708
BstNSI	RCATGY	1 cut(s)	1033
BstSCI	CCNGG	2 cut(s)	448, 706
BstSFI	CTRYAG	1 cut(s)	876
BstV1I	GCAGC	7 cut(s)	51, 411, 414, 571, 580, 890, 1023
BstV2I	GAAGAC	2 cut(s)	175, 342
Bsu36I	CCTNAGG	1 cut(s)	699
BsuRI	GGCC	4 cut(s)	93, 130, 798, 808
BtrI	CACGTC	1 cut(s)	464
BtsIMutI	CAGTG	1 cut(s)	791
Cac8I	GCNNGC	6 cut(s)	132, 455, 476, 758, 796, 854
CaiI	CAGNNNCTG	1 cut(s)	408
CfoI	GCGC	1 cut(s)	840
Cfr13I	GGNCC	4 cut(s)	92, 129, 797, 806
CseI	GACGC	2 cut(s)	182, 467
Csp6I	GTAC	1 cut(s)	868
CspCI	CAANNNNNGTGG	2 cut(s)	811, 846
CviAII	CATG	3 cut(s)	67, 307, 1030
CviQI	GTAC	1 cut(s)	868
DdeI	CTNAG	4 cut(s)	609, 621, 699, 864
DpnI	GATC	2 cut(s)	5, 439
DpnII	GATC	2 cut(s)	3, 437
DrdI	GACNNNNNNGTC	1 cut(s)	179
DseDI	GACNNNNNNGTC	1 cut(s)	179
Eam1104I	CTCTTC	3 cut(s)	231, 567, 863
EarI	CTCTTC	3 cut(s)	231, 567, 863
EciI	GGCGGA	3 cut(s)	103, 133, 309
Ecl136II	GAGCTC	1 cut(s)	220
Eco130I	CCWWGG	3 cut(s)	333, 591, 945
Eco24I	GRGCYC	1 cut(s)	222
Eco31I	GGTCTC	1 cut(s)	291
Eco53kI	GAGCTC	1 cut(s)	220
Eco57I	CTGAAG	1 cut(s)	548
Eco81I	CCTNAGG	1 cut(s)	699
Eco88I	CYCGRG	2 cut(s)	412, 932
EcoICRI	GAGCTC	1 cut(s)	220
EcoNI	CCTNNNNNAGG	1 cut(s)	68
EcoRI	GAATTC	1 cut(s)	277
EcoRII	CCWGG	2 cut(s)	448, 706
EcoT14I	CCWWGG	3 cut(s)	333, 591, 945
EcoT38I	GRGCYC	1 cut(s)	222
ErhI	CCWWGG	3 cut(s)	333, 591, 945
FaeI	CATG	3 cut(s)	70, 310, 1033
FaiI	YATR	6 cut(s)	68, 234, 308, 820, 921, 1031
FaqI	GGGAC	1 cut(s)	474
FatI	CATG	3 cut(s)	66, 306, 1029
FauI	CCCGC	3 cut(s)	103, 139, 450
FbaI	TGATCA	1 cut(s)	3
FblI	GTMKAC	2 cut(s)	21, 78
FriOI	GRGCYC	1 cut(s)	222
FspBI	CTAG	2 cut(s)	468, 882
GlaI	GCGC	1 cut(s)	839
GsuI	CTGGAG	1 cut(s)	358
HaeIII	GGCC	4 cut(s)	93, 130, 798, 808
HapII	CCGG	1 cut(s)	189
HgaI	GACGC	2 cut(s)	182, 467
HhaI	GCGC	1 cut(s)	840
Hin1I	GRCGYC	2 cut(s)	75, 174
Hin1II	CATG	3 cut(s)	70, 310, 1033
Hin6I	GCGC	1 cut(s)	838
HinP1I	GCGC	1 cut(s)	838
HincII	GTYRAC	3 cut(s)	22, 79, 340
HindII	GTYRAC	3 cut(s)	22, 79, 340
HinfI	GANTC	7 cut(s)	80, 200, 323, 347, 605, 623, 684
HpaII	CCGG	1 cut(s)	189
HphI	GGTGA	3 cut(s)	209, 295, 649
Hpy166II	GTNNAC	4 cut(s)	22, 79, 340, 970
Hpy188I	TCNGA	7 cut(s)	205, 217, 283, 547, 604, 610, 999
Hpy188III	TCNNGA	6 cut(s)	101, 170, 367, 932, 934, 971
Hpy8I	GTNNAC	4 cut(s)	22, 79, 340, 970
Hpy99I	CGWCG	5 cut(s)	23, 26, 62, 80, 176
HpyAV	CCTTC	3 cut(s)	127, 428, 919
HpyCH4III	ACNGT	4 cut(s)	107, 197, 363, 872
HpyCH4IV	ACGT	2 cut(s)	75, 463
HpyCH4V	TGCA	7 cut(s)	247, 512, 736, 760, 822, 878, 907
HpyF10VI	GCNNNNNNNGC	3 cut(s)	143, 152, 408
HpyF3I	CTNAG	4 cut(s)	609, 621, 699, 864
HpySE526I	ACGT	2 cut(s)	75, 463
Hsp92I	GRCGYC	2 cut(s)	75, 174
Hsp92II	CATG	3 cut(s)	70, 310, 1033
HspAI	GCGC	1 cut(s)	838
Ksp22I	TGATCA	1 cut(s)	3
Kzo9I	GATC	2 cut(s)	3, 437
LguI	GCTCTTC	1 cut(s)	863
LmnI	GCTCC	9 cut(s)	103, 160, 225, 410, 451, 592, 664, 712, 740
Lsp1109I	GCAGC	7 cut(s)	51, 411, 414, 571, 580, 890, 1023
LweI	GCATC	1 cut(s)	633
MaeI	CTAG	2 cut(s)	468, 882
MaeII	ACGT	2 cut(s)	75, 463
MaeIII	GTNAC	2 cut(s)	107, 197
MalI	GATC	2 cut(s)	5, 439
MbiI	CCGCTC	2 cut(s)	98, 155
MboI	GATC	2 cut(s)	3, 437
MfeI	CAATTG	1 cut(s)	441
MhlI	GDGCHC	1 cut(s)	222
MluCI	AATT	5 cut(s)	277, 441, 513, 744, 913
MlyI	GAGTC	6 cut(s)	74, 194, 332, 356, 614, 632
MmeI	TCCRAC	3 cut(s)	228, 288, 911
MroXI	GAANNNNTTC	1 cut(s)	993
MslI	CAYNNNNRTG	2 cut(s)	386, 784
MspI	CCGG	1 cut(s)	189
MspR9I	CCNGG	2 cut(s)	450, 708
MunI	CAATTG	1 cut(s)	441
Mva1269I	GAATGC	1 cut(s)	571
MvaI	CCWGG	2 cut(s)	450, 708
MwoI	GCNNNNNNNGC	3 cut(s)	143, 152, 408
NdeII	GATC	2 cut(s)	3, 437
NlaIII	CATG	3 cut(s)	70, 310, 1033
NlaIV	GGNNCC	3 cut(s)	94, 705, 807
NmeAIII	GCCGAG	1 cut(s)	69
NmuCI	GTSAC	1 cut(s)	197
NspI	RCATGY	1 cut(s)	1033
OliI	CACNNNNGTG	1 cut(s)	784
PaeR7I	CTCGAG	1 cut(s)	932
PciI	ACATGT	1 cut(s)	1029
PciSI	GCTCTTC	1 cut(s)	863
PcsI	WCGNNNNNNNCGW	3 cut(s)	54, 81, 201
PctI	GAATGC	1 cut(s)	571
PdmI	GAANNNNTTC	1 cut(s)	993
PfeI	GAWTC	1 cut(s)	684
PflFI	GACNNNGTC	1 cut(s)	83
PleI	GAGTC	6 cut(s)	74, 194, 331, 355, 613, 631
PpsI	GAGTC	6 cut(s)	74, 194, 331, 355, 613, 631
PscI	ACATGT	1 cut(s)	1029
Psp124BI	GAGCTC	1 cut(s)	222
Psp6I	CCWGG	2 cut(s)	448, 706
PspGI	CCWGG	2 cut(s)	448, 706
PspN4I	GGNNCC	3 cut(s)	94, 705, 807
PspPI	GGNCC	4 cut(s)	92, 129, 797, 806
PstI	CTGCAG	1 cut(s)	880
PstNI	CAGNNNCTG	1 cut(s)	408
PsyI	GACNNNGTC	1 cut(s)	83
RsaI	GTAC	1 cut(s)	869
RsaNI	GTAC	1 cut(s)	868
RseI	CAYNNNNRTG	2 cut(s)	386, 784
SacI	GAGCTC	1 cut(s)	222
SalI	GTCGAC	2 cut(s)	20, 77
SapI	GCTCTTC	1 cut(s)	863
Sau3AI	GATC	2 cut(s)	3, 437
Sau96I	GGNCC	4 cut(s)	92, 129, 797, 806
SchI	GAGTC	6 cut(s)	74, 194, 332, 356, 614, 632
ScrFI	CCNGG	2 cut(s)	450, 708
SduI	GDGCHC	1 cut(s)	222
SfaNI	GCATC	1 cut(s)	633
SfcI	CTRYAG	1 cut(s)	876
Sfr274I	CTCGAG	1 cut(s)	932
SgrDI	CGTCGACG	1 cut(s)	20
SlaI	CTCGAG	1 cut(s)	932
SmiMI	CAYNNNNRTG	2 cut(s)	386, 784
SmlI	CTYRAG	1 cut(s)	932
SmoI	CTYRAG	1 cut(s)	932
Sse9I	AATT	5 cut(s)	277, 441, 513, 744, 913
SspMI	CTAG	2 cut(s)	468, 882
SstI	GAGCTC	1 cut(s)	222
StyD4I	CCNGG	2 cut(s)	448, 706
StyI	CCWWGG	3 cut(s)	333, 591, 945
TaaI	ACNGT	4 cut(s)	107, 197, 363, 872
TaiI	ACGT	2 cut(s)	78, 466
TaqI	TCGA	5 cut(s)	21, 48, 57, 78, 933
TasI	AATT	5 cut(s)	277, 441, 513, 744, 913
TauI	GCSGC	6 cut(s)	39, 137, 149, 152, 155, 179
TfiI	GAWTC	1 cut(s)	684
TscAI	CASTG	1 cut(s)	791
TseFI	GTSAC	1 cut(s)	197
TseI	GCWGC	7 cut(s)	39, 399, 402, 568, 584, 878, 1011
Tsp45I	GTSAC	1 cut(s)	197
TspDTI	ATGAA	3 cut(s)	249, 323, 564
TspGWI	ACGGA	2 cut(s)	73, 219
TspRI	CASTG	1 cut(s)	791
Tth111I	GACNNNGTC	1 cut(s)	83
XagI	CCTNNNNNAGG	1 cut(s)	68
XapI	RAATTY	1 cut(s)	277
XceI	RCATGY	1 cut(s)	1033
XhoI	CTCGAG	1 cut(s)	932
XmiI	GTMKAC	2 cut(s)	21, 78
XmnI	GAANNNNTTC	1 cut(s)	993
XspI	CTAG	2 cut(s)	468, 882
ZraI	GACGTC	1 cut(s)	76

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.