Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0009110

Physical Location & Seq

Genomic Coordinates Reverse (-)

16163568 .. 16172590

Gene Structure 9023 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g19690.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2715 bp

ATGAAGTTCACCTTGTGTTCGTCATCTTCAGAGCTTAGATGTCCTTTTGTTGAGGGGTTGCCCCGATGTATAAACAAGAATATGCTAGCGCCTGGACTGCCAGTGAAGGTTGATAATTTTGTTAAGGAGAACTCTCAAGCATCTAACCATGATACTAGGAAGCTGGTGATGTCTAGCAATTTGATGCCTCGGCGCATAAATGAATCACGTTTGGGAAAGAAACAACTGGAGGAGCCAGGTCGGAAGTTGGAATATGTGAGGACATTATTGATTGACAACTATGATAGCTACACATACAATATATACCAGGAGCTTTCTGTCATTAATGGCTTACCTCCTGTTGTGGTGCGAAATGATGAGTTGTCATGGAAAGATGTTTGCCATTACTTGTATGAAGAAAATGCATTTGATAACATTGTTATATCACCTGGACCAGGTTCTCCTACATGCCCAGCTGACATAGGAATATGTCTTCAGGTGCTGCTTGACTGCTGGAATGTTCCTATCTTGGGTGTTTGCCTTGGACATCAGGCTTTAGGTTATGTGCATGGTGCTAAAGTTGTCCACGCATCTGAACCGGTCCATGGACGCTTGAGTGAAATTCAGCACAATGGATGTGTACTTTTCAATGACATCCCGTCTGGCCACAACTCTGGATTTAAGGTCGTCCGGTACCATTCACTGGTCATAGATGCTGAATCACTCCCAGATGAACTCATTCCTATAGCATGGACTTCTTCCATGAATGCACTTTCTTTTATTGAGACCAAAGAATCTGATGTTGCCTCTAAATCTGCCGATGCGTCTTTTTTAATGAAACAAAAAAATTGTACTTATTCACCTTCCAGCCATTCTGGCAAAGTACAGAGTGAAAGGGTTCTCATGGGCATTATGCATTCTTCCAGGCCTCATTATGGTGTACAGTTCCATCCAGAGAGTGTTGCTACTTGCCATGGAAGGCAGATATTCAAGAATTTTAGAAAGATCACAGAGGATTATTGGCTGAGATCGAGGGCATCATTCATCAACGCGCAAAATTTTCCTTTTACTGCACGTATGCAGATGCCCCATGTTGGTCAACTATTGACAGACGTTCCTAAACATCGGCAACTACTAAATAATGCAGACGGTCACTTATATAAAGATGCAGACAACGTGATTTGCTCTGGCATGGTTGATATGGTAAACCTTTTGCATCCAAGCATTACTGTTAAAGATCTGAGGTTGACATGGAAGAAATTGAAGCACTTGGCCGGTCAAGTTGGTGGAGCAAGAAATATATTTTGTGAACTGTATGGACAAGATAAAGCTGAAAACACCTTTTGGTTGGACAGTTCATCAATAGAGAAGAGAAGAGCACGATTTTCATTTATGGGAGGAAAAGGTGGAGCTCTTTGGAAGCAAGTGACCTTTAAATTGTCTGATAAAAGTGATACGACTTTAAAGGGCAGAGGTCTTCTATCTGTTCAGGATGCTCAAGGCTCTATCAAACGTTCATTTTTGGAAGAAGGATTTCTTGATTTTTTGAAGAAGGGGCTCCTGTCATTTTGTTATGATGAAAAGGAATATGAAGAACTTCCATTCGATTTTCATGGTGGATATATTGGTTACCTCGGGTATAACCTAAAAGTTGAATGTGGTGTGGATTCTAATCGCCACAGATCCCAGACTCCAGATGCTTGCTTTTTCTTTGCTGATAACCTTGTAGTTGTTGATCACCGAAATGATGATGTTTACATATTGTCTGTAGACGAAGTATGCAGACCTTTGACACCTTGGTTGGATGACACAGAACAGAAGCTTCTTAACTTAAAAGCTTCTGCTACAGGGGAGGGAAAGAAGCCAACTTTAACGGCTCTAAGATCTTCGTCCGGTCAAGGAACTTTTCTTGGTGATAAATCAAGAGAAGCGTATATAGAAGATGTGGACAAGTGCCTGGAATACATTAAAGATGGTGAAAGTTATGAATTGTGTCTGACAACTCAGATGAGAAAAACAATTGGGGATATAGACTCTTTGGGACTTTACCTTCATTTAAGAGAAAAGAATCCAGCACCATATGCTGCCTGGCTCAATTTTTCAGATGAAGACCTATCTATCTGCTGTTCTTCCCCAGAGCGTTTCTTACAATTGGATAGAAATGGTGTACTCGAAGCAAAGCCCATTAAGGGTACTGTAGCTCGTGGTGCAACACCAGAGGAAGATGAACAACGTAAACTACAGCTGCAATATAGTGAAAAGGATCAAGCTGAAAATCTGATGATAGTAGATCTTTTAAGGAATGACCTTGGTCGTGTGTGTGAGCCAGGCTCAGTTCATGTGCCACGTCTTATGGATGTGGAATCATATGCTACAGTTCATACTATGGTCAGCACAATTCGTGGGCAAAAACGGTCTGATATGACTGCAATTGATTGTGTGAGAGCAGCATTTCCGGGTGGTTCGATGACGGGTGCACCTAAATTGAGATCAATGGAACTTCTTGATTCAATCGAAAGTAGTTCTCGAGGTATCTACTCGGGCTCCATTGGATTTTTCTCATATAACCAGACATTTGATCTAAATATAGTTATTAGAACTGTTGTTATACACAAGGGTGAAGCTTCAATAGGAGCTGGGGGAGCCATTGTTGCCTTATCAAATCCTGAAGACGAATACGAGGAAATGGTTTTGAAAACTCGAGCTCCAGCAAAAGCTGTACTGGAATTTTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006766 GO:0006767 GO:0006807 GO:0008150 GO:0008152 GO:0008153 GO:0008483 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009108 GO:0009110 GO:0009396 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016769 GO:0017144 GO:0018130 GO:0019438 GO:0019752 GO:0032787 GO:0034641 GO:0042364 GO:0042398 GO:0042537 GO:0042558 GO:0042559 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046417 GO:0046482 GO:0046483 GO:0046653 GO:0046654 GO:0046655 GO:0046656 GO:0046820 GO:0051186 GO:0051188 GO:0071704 GO:0072330 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00790 map00790

Pfam Domains

Protein Families

Anth_synt_I_N Chorismate_bind GATase

Protein Analysis

905

Amino Acids

101.07

Weight (kDa)

6.14

Isoelectric Point (pI)

45.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase	PF00117	89 - 252	4.4e-30	Glutamine amidotransferase class-I
GATase	PF00117	289 - 326	4.1e-08	Glutamine amidotransferase class-I
Anth_synt_I_N	PF04715	433 - 580	6.3e-17	Anthranilate synthase component I, N terminal region
Chorismate_bind	PF00425	635 - 892	2.6e-86	chorismate binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28880
fragaria_vesca	FvH4_7g19690
malus_domestica	MD01G1109300.v1.1 MD07G1175300.v1.1
prunus_persica	Prupe.2G214500_v2.0.a1
pyrus_communis	pycom01g13660 pycom07g16970
rosa_chinensis	RchiOBHm_Chr1g0363521
rosa_laevigata	RLG00000027559
rosa_multiflora	Rmu_ssc0000433.1_g000001
rosa_roxburghii	Rroxscaffold_4G00293090
rosa_rugosa	Rorug01G0308100
rosa_samantha	Rh1AG316800 Rh1BG280200 Rh1CG296300 Rh1DG311400 Rh5DG228600
rosa_wichuraiana	Rw0G000800 Rw1G028080 Rw2G022180 Rw3G029370 Rw5G038670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	672
AccB1I	GGYRCC	1 cut(s)	672
AccB7I	CCANNNNNTGG	1 cut(s)	682
AccI	GTMKAC	1 cut(s)	1749
AccII	CGCG	1 cut(s)	1031
AclI	AACGTT	1 cut(s)	1492
AclWI	GGATC	2 cut(s)	1656, 2250
AcoI	YGGCCR	2 cut(s)	643, 1251
AcsI	RAATTY	4 cut(s)	600, 973, 1036, 2705
AcuI	CTGAAG	3 cut(s)	12, 458, 2667
AdeI	CACNNNGTG	1 cut(s)	15
AfaI	GTAC	8 cut(s)	621, 674, 832, 864, 921, 2148, 2173, 2700
AfiI	CCNNNNNNNGG	7 cut(s)	434, 509, 584, 682, 914, 1073, 2168
AgeI	ACCGGT	1 cut(s)	577
AgsI	TTSAA	8 cut(s)	628, 970, 1243, 1528, 1634, 2490, 2607, 2674
AhdI	GACNNNNNGTC	1 cut(s)	637
AjiI	CACGTC	1 cut(s)	2327
AjnI	CCWGG	9 cut(s)	91, 235, 306, 427, 433, 902, 1935, 2066, 2305
AleI	CACNNNNGTG	1 cut(s)	2595
Alw21I	GWGCWC	4 cut(s)	1360, 1393, 2458, 2686
Alw26I	GTCTC	1 cut(s)	758
Alw44I	GTGCAC	1 cut(s)	2454
AlwI	GGATC	2 cut(s)	1656, 2250
AlwNI	CAGNNNCTG	1 cut(s)	481
Ama87I	CYCGRG	4 cut(s)	1613, 2505, 2518, 2679
AoxI	GGCC	3 cut(s)	643, 905, 1251
ApaLI	GTGCAC	1 cut(s)	2454
ApeKI	GCWGC	4 cut(s)	481, 2063, 2224, 2426
ApoI	RAATTY	4 cut(s)	600, 973, 1036, 2705
AseI	ATTAAT	1 cut(s)	324
AsiGI	ACCGGT	1 cut(s)	577
Asp700I	GAANNNNTTC	4 cut(s)	717, 876, 1512, 1575
Asp718I	GGTACC	1 cut(s)	672
AspLEI	GCGC	3 cut(s)	91, 195, 1033
AspS9I	GGNCC	2 cut(s)	431, 580
AsuC2I	CCSGG	1 cut(s)	2436
AsuHPI	GGTGA	7 cut(s)	178, 417, 831, 1709, 1904, 1967, 2609
AsuNHI	GCTAGC	1 cut(s)	85
AvaI	CYCGRG	4 cut(s)	1613, 2505, 2518, 2679
AvaII	GGWCC	2 cut(s)	431, 580
BaeGI	GKGCMC	1 cut(s)	2458
BalI	TGGCCA	1 cut(s)	645
BanI	GGYRCC	1 cut(s)	672
BanII	GRGCYC	4 cut(s)	1393, 1539, 2525, 2686
BauI	CACGAG	1 cut(s)	2181
BbsI	GAAGAC	4 cut(s)	464, 1448, 2094, 2655
Bbv12I	GWGCWC	4 cut(s)	1360, 1393, 2458, 2686
BbvI	GCAGC	4 cut(s)	468, 2050, 2211, 2438
BccI	CCATC	2 cut(s)	936, 1946
BceAI	ACGGC	1 cut(s)	1869
BciT130I	CCWGG	9 cut(s)	93, 237, 308, 429, 435, 904, 1937, 2068, 2307
BclI	TGATCA	1 cut(s)	1714
BcnI	CCSGG	1 cut(s)	2436
BcoDI	GTCTC	1 cut(s)	758
BfaI	CTAG	3 cut(s)	86, 156, 174
BfmI	CTRYAG	6 cut(s)	723, 1746, 1824, 2175, 2219, 2352
BfoI	RGCGCY	1 cut(s)	92
BglI	GCCNNNNNGGC	1 cut(s)	855
BglII	AGATCT	3 cut(s)	1216, 1862, 2269
BisI	GCNGC	4 cut(s)	482, 2064, 2225, 2427
BlsI	GCNGC	4 cut(s)	483, 2065, 2226, 2428
Bme18I	GGWCC	2 cut(s)	431, 580
BmeRI	GACNNNNNGTC	1 cut(s)	637
BmeT110I	CYCGRG	4 cut(s)	1613, 2505, 2518, 2679
BmgBI	CACGTC	1 cut(s)	2327
BmgT120I	GGNCC	2 cut(s)	431, 580
BmiI	GGNNCC	5 cut(s)	234, 674, 1538, 2524, 2623
BmtI	GCTAGC	1 cut(s)	89
BoxI	GACNNNNGTC	1 cut(s)	2289
BpiI	GAAGAC	4 cut(s)	464, 1448, 2094, 2655
BplI	GAGNNNNNCTC	2 cut(s)	2294, 2326
BpmI	CTGGAG	3 cut(s)	248, 1656, 2670
BpuEI	CTTGAG	3 cut(s)	120, 613, 1461
BpuMI	CCSGG	1 cut(s)	2436
BsaAI	YACGTR	1 cut(s)	1055
BsaBI	GATNNNNATC	1 cut(s)	1650
BsaI	GGTCTC	1 cut(s)	758
BsaJI	CCNNGG	7 cut(s)	188, 520, 583, 952, 1612, 1775, 2287
BsaWI	WCCGGW	3 cut(s)	577, 669, 1871
BsaXI	ACNNNNNCTCC	6 cut(s)	2507, 2537, 2613, 2643, 2668, 2698
Bsc4I	CCNNNNNNNGG	7 cut(s)	434, 509, 584, 682, 914, 1073, 2168
Bse118I	RCCGGY	2 cut(s)	577, 1253
Bse1I	ACTGG	4 cut(s)	101, 231, 687, 2706
Bse8I	GATNNNNATC	1 cut(s)	1650
BseBI	CCWGG	9 cut(s)	93, 237, 308, 429, 435, 904, 1937, 2068, 2307
BseDI	CCNNGG	7 cut(s)	188, 520, 583, 952, 1612, 1775, 2287
BseGI	GGATG	7 cut(s)	620, 633, 928, 1195, 1477, 1789, 2341
BseJI	GATNNNNATC	1 cut(s)	1650
BseLI	CCNNNNNNNGG	7 cut(s)	434, 509, 584, 682, 914, 1073, 2168
BseMII	CTCAG	4 cut(s)	995, 1211, 1997, 2325
BseNI	ACTGG	4 cut(s)	101, 231, 687, 2706
BseRI	GAGGAG	1 cut(s)	245
BseSI	GKGCMC	1 cut(s)	2458
BseXI	GCAGC	4 cut(s)	468, 2050, 2211, 2438
BseYI	CCCAGC	2 cut(s)	451, 2615
BsgI	GTGCAG	1 cut(s)	1035
Bsh1236I	CGCG	1 cut(s)	1031
BshFI	GGCC	3 cut(s)	645, 907, 1253
BshNI	GGYRCC	1 cut(s)	672
BshTI	ACCGGT	1 cut(s)	577
BsiHKAI	GWGCWC	4 cut(s)	1360, 1393, 2458, 2686
BsiHKCI	CYCGRG	4 cut(s)	1613, 2505, 2518, 2679
BsiSI	CCGG	5 cut(s)	578, 670, 1254, 1872, 2435
BslFI	GGGAC	1 cut(s)	2034
BslI	CCNNNNNNNGG	7 cut(s)	434, 509, 584, 682, 914, 1073, 2168
BsmAI	GTCTC	1 cut(s)	758
BsmFI	GGGAC	1 cut(s)	2034
BsmI	GAATGC	2 cut(s)	751, 895
BsnI	GGCC	3 cut(s)	645, 907, 1253
Bso31I	GGTCTC	1 cut(s)	758
BsoBI	CYCGRG	4 cut(s)	1613, 2505, 2518, 2679
Bsp1286I	GDGCHC	6 cut(s)	1360, 1393, 1539, 2458, 2525, 2686
Bsp1407I	TGTACA	1 cut(s)	919
Bsp19I	CCATGG	2 cut(s)	583, 952
BspANI	GGCC	3 cut(s)	645, 907, 1253
BspCNI	CTCAG	4 cut(s)	996, 1212, 1996, 2324
BspFNI	CGCG	1 cut(s)	1031
BspLI	GGNNCC	5 cut(s)	234, 674, 1538, 2524, 2623
BspOI	GCTAGC	1 cut(s)	89
BspPI	GGATC	2 cut(s)	1656, 2250
BspQI	GCTCTTC	1 cut(s)	1348
BspT107I	GGYRCC	1 cut(s)	672
BspTNI	GGTCTC	1 cut(s)	758
BsrFI	RCCGGY	2 cut(s)	577, 1253
BsrGI	TGTACA	1 cut(s)	919
BsrI	ACTGG	4 cut(s)	101, 231, 687, 2706
BssAI	RCCGGY	2 cut(s)	577, 1253
BssECI	CCNNGG	7 cut(s)	188, 520, 583, 952, 1612, 1775, 2287
BssSI	CACGAG	1 cut(s)	2181
BssT1I	CCWWGG	5 cut(s)	520, 583, 952, 1775, 2287
Bst2BI	CACGAG	1 cut(s)	2181
Bst2UI	CCWGG	9 cut(s)	93, 237, 308, 429, 435, 904, 1937, 2068, 2307
Bst4CI	ACNGT	9 cut(s)	924, 1130, 1210, 1293, 1334, 2176, 2356, 2394, 2581
Bst6I	CTCTTC	2 cut(s)	1343, 1348
BstAPI	GCANNNNNTGC	1 cut(s)	2060
BstAUI	TGTACA	1 cut(s)	919
BstBAI	YACGTR	1 cut(s)	1055
BstC8I	GCNNGC	2 cut(s)	87, 1681
BstDEI	CTNAG	6 cut(s)	35, 1004, 1220, 1859, 1983, 2311
BstDSI	CCRYGG	2 cut(s)	583, 952
BstEII	GGTNACC	1 cut(s)	1607
BstENI	CCTNNNNNAGG	1 cut(s)	432
BstF5I	GGATG	7 cut(s)	620, 633, 928, 1195, 1477, 1789, 2341
BstFNI	CGCG	1 cut(s)	1031
BstH2I	RGCGCY	1 cut(s)	92
BstHHI	GCGC	3 cut(s)	91, 195, 1033
BstMAI	GTCTC	1 cut(s)	758
BstMWI	GCNNNNNNNGC	6 cut(s)	97, 855, 2060, 2186, 2621, 2630
BstNI	CCWGG	9 cut(s)	93, 237, 308, 429, 435, 904, 1937, 2068, 2307
BstNSI	RCATGY	1 cut(s)	450
BstPAI	GACNNNNGTC	1 cut(s)	2289
BstPI	GGTNACC	1 cut(s)	1607
BstSFI	CTRYAG	6 cut(s)	723, 1746, 1824, 2175, 2219, 2352
BstSLI	GKGCMC	1 cut(s)	2458
BstUI	CGCG	1 cut(s)	1031
BstV1I	GCAGC	4 cut(s)	468, 2050, 2211, 2438
BstV2I	GAAGAC	4 cut(s)	464, 1448, 2094, 2655
BstX2I	RGATCY	4 cut(s)	1216, 1661, 1862, 2269
BstXI	CCANNNNNNTGG	1 cut(s)	653
BstYI	RGATCY	4 cut(s)	1216, 1661, 1862, 2269
BsuRI	GGCC	3 cut(s)	645, 907, 1253
BtgI	CCRYGG	2 cut(s)	583, 952
BtrI	CACGTC	1 cut(s)	2327
BtsCI	GGATG	7 cut(s)	620, 633, 928, 1195, 1477, 1789, 2341
BtsIMutI	CAGTG	2 cut(s)	108, 680
Cac8I	GCNNGC	2 cut(s)	87, 1681
CaiI	CAGNNNCTG	1 cut(s)	481
CfoI	GCGC	3 cut(s)	91, 195, 1033
Cfr10I	RCCGGY	2 cut(s)	577, 1253
Cfr13I	GGNCC	2 cut(s)	431, 580
CseI	GACGC	2 cut(s)	597, 792
CsiI	ACCWGGT	1 cut(s)	433
Csp6I	GTAC	8 cut(s)	620, 673, 831, 863, 920, 2147, 2172, 2699
CspAI	ACCGGT	1 cut(s)	577
CviQI	GTAC	8 cut(s)	620, 673, 831, 863, 920, 2147, 2172, 2699
DdeI	CTNAG	6 cut(s)	35, 1004, 1220, 1859, 1983, 2311
DraI	TTTAAA	2 cut(s)	1414, 1443
DraIII	CACNNNGTG	1 cut(s)	15
DriI	GACNNNNNGTC	1 cut(s)	637
EaeI	YGGCCR	2 cut(s)	643, 1251
Eam1104I	CTCTTC	2 cut(s)	1343, 1348
Eam1105I	GACNNNNNGTC	1 cut(s)	637
EarI	CTCTTC	2 cut(s)	1343, 1348
Ecl136II	GAGCTC	2 cut(s)	1391, 2684
Eco130I	CCWWGG	5 cut(s)	520, 583, 952, 1775, 2287
Eco147I	AGGCCT	1 cut(s)	907
Eco24I	GRGCYC	4 cut(s)	1393, 1539, 2525, 2686
Eco31I	GGTCTC	1 cut(s)	758
Eco47I	GGWCC	2 cut(s)	431, 580
Eco53kI	GAGCTC	2 cut(s)	1391, 2684
Eco57I	CTGAAG	3 cut(s)	12, 458, 2667
Eco88I	CYCGRG	4 cut(s)	1613, 2505, 2518, 2679
Eco91I	GGTNACC	1 cut(s)	1607
EcoICRI	GAGCTC	2 cut(s)	1391, 2684
EcoNI	CCTNNNNNAGG	1 cut(s)	432
EcoO65I	GGTNACC	1 cut(s)	1607
EcoRII	CCWGG	9 cut(s)	91, 235, 306, 427, 433, 902, 1935, 2066, 2305
EcoT14I	CCWWGG	5 cut(s)	520, 583, 952, 1775, 2287
EcoT22I	ATGCAT	2 cut(s)	406, 897
EcoT38I	GRGCYC	4 cut(s)	1393, 1539, 2525, 2686
ErhI	CCWWGG	5 cut(s)	520, 583, 952, 1775, 2287
FalI	AAGNNNNNCTT	3 cut(s)	28, 1500, 1532
FaqI	GGGAC	1 cut(s)	2034
FauNDI	CATATG	2 cut(s)	2059, 2347
FbaI	TGATCA	1 cut(s)	1714
FblI	GTMKAC	1 cut(s)	1749
Fnu4HI	GCNGC	4 cut(s)	482, 2064, 2225, 2427
FokI	GGATG	7 cut(s)	620, 627, 915, 1182, 1484, 1796, 2348
FriOI	GRGCYC	4 cut(s)	1393, 1539, 2525, 2686
Fsp4HI	GCNGC	4 cut(s)	482, 2064, 2225, 2427
FspBI	CTAG	3 cut(s)	86, 156, 174
GlaI	GCGC	3 cut(s)	90, 194, 1032
GluI	GCNGC	4 cut(s)	482, 2064, 2225, 2427
GsaI	CCCAGC	2 cut(s)	455, 2619
GsuI	CTGGAG	3 cut(s)	248, 1656, 2670
HaeII	RGCGCY	1 cut(s)	92
HaeIII	GGCC	3 cut(s)	645, 907, 1253
HapII	CCGG	5 cut(s)	578, 670, 1254, 1872, 2435
HgaI	GACGC	2 cut(s)	597, 792
HhaI	GCGC	3 cut(s)	91, 195, 1033
Hin6I	GCGC	3 cut(s)	89, 193, 1031
HinP1I	GCGC	3 cut(s)	89, 193, 1031
HincII	GTYRAC	2 cut(s)	1079, 1227
HindII	GTYRAC	2 cut(s)	1079, 1227
HindIII	AAGCTT	3 cut(s)	1799, 1815, 2601
HinfI	GANTC	9 cut(s)	203, 698, 773, 1646, 1669, 2012, 2047, 2342, 2486
HpaII	CCGG	5 cut(s)	578, 670, 1254, 1872, 2435
HphI	GGTGA	7 cut(s)	178, 417, 831, 1709, 1904, 1967, 2609
HpyAV	CCTTC	6 cut(s)	100, 852, 951, 1502, 1525, 2039
HpyCH4III	ACNGT	9 cut(s)	924, 1130, 1210, 1293, 1334, 2176, 2356, 2394, 2581
HpyCH4IV	ACGT	7 cut(s)	208, 1054, 1092, 1155, 1492, 2212, 2326
HpyF10VI	GCNNNNNNNGC	6 cut(s)	97, 855, 2060, 2186, 2621, 2630
HpyF3I	CTNAG	6 cut(s)	35, 1004, 1220, 1859, 1983, 2311
HpySE526I	ACGT	7 cut(s)	208, 1054, 1092, 1155, 1492, 2212, 2326
HspAI	GCGC	3 cut(s)	89, 193, 1031
KpnI	GGTACC	1 cut(s)	676
Ksp22I	TGATCA	1 cut(s)	1714
LguI	GCTCTTC	1 cut(s)	1348
LmnI	GCTCC	9 cut(s)	232, 310, 1268, 1388, 1542, 2528, 2612, 2621, 2689
Lsp1109I	GCAGC	4 cut(s)	468, 2050, 2211, 2438
MabI	ACCWGGT	1 cut(s)	433
MaeI	CTAG	3 cut(s)	86, 156, 174
MaeII	ACGT	7 cut(s)	208, 1054, 1092, 1155, 1492, 2212, 2326
MaeIII	GTNAC	3 cut(s)	1130, 1405, 1607
MfeI	CAATTG	3 cut(s)	1998, 2129, 2409
MflI	RGATCY	4 cut(s)	1216, 1661, 1862, 2269
MhlI	GDGCHC	6 cut(s)	1360, 1393, 1539, 2458, 2525, 2686
MlsI	TGGCCA	1 cut(s)	645
MluNI	TGGCCA	1 cut(s)	645
MlyI	GAGTC	2 cut(s)	1663, 2006
MmeI	TCCRAC	4 cut(s)	221, 228, 1308, 1761
Mox20I	TGGCCA	1 cut(s)	645
Mph1103I	ATGCAT	2 cut(s)	406, 897
MroXI	GAANNNNTTC	4 cut(s)	717, 876, 1512, 1575
MscI	TGGCCA	1 cut(s)	645
MslI	CAYNNNNRTG	3 cut(s)	915, 1722, 2595
Msp20I	TGGCCA	1 cut(s)	645
MspA1I	CMGCKG	2 cut(s)	455, 2224
MspI	CCGG	5 cut(s)	578, 670, 1254, 1872, 2435
MunI	CAATTG	3 cut(s)	1998, 2129, 2409
Mva1269I	GAATGC	2 cut(s)	751, 895
MvaI	CCWGG	9 cut(s)	93, 237, 308, 429, 435, 904, 1937, 2068, 2307
MvnI	CGCG	1 cut(s)	1031
MwoI	GCNNNNNNNGC	6 cut(s)	97, 855, 2060, 2186, 2621, 2630
NciI	CCSGG	1 cut(s)	2436
NcoI	CCATGG	2 cut(s)	583, 952
NdeI	CATATG	2 cut(s)	2059, 2347
NheI	GCTAGC	1 cut(s)	85
NlaIV	GGNNCC	5 cut(s)	234, 674, 1538, 2524, 2623
NmeAIII	GCCGAG	1 cut(s)	169
NmuCI	GTSAC	2 cut(s)	1130, 1405
NsiI	ATGCAT	2 cut(s)	406, 897
NspI	RCATGY	1 cut(s)	450
OliI	CACNNNNGTG	1 cut(s)	2595
PaeR7I	CTCGAG	2 cut(s)	2505, 2679
PceI	AGGCCT	1 cut(s)	907
PciSI	GCTCTTC	1 cut(s)	1348
PctI	GAATGC	2 cut(s)	751, 895
PdmI	GAANNNNTTC	4 cut(s)	717, 876, 1512, 1575
PfeI	GAWTC	7 cut(s)	203, 698, 773, 1646, 2047, 2342, 2486
PflMI	CCANNNNNTGG	1 cut(s)	682
PinAI	ACCGGT	1 cut(s)	577
PkrI	GCNGC	4 cut(s)	483, 2065, 2226, 2428
PleI	GAGTC	2 cut(s)	1663, 2006
PpsI	GAGTC	2 cut(s)	1663, 2006
Ppu21I	YACGTR	1 cut(s)	1055
PshAI	GACNNNNGTC	1 cut(s)	2289
PshBI	ATTAAT	1 cut(s)	324
Psp124BI	GAGCTC	2 cut(s)	1393, 2686
Psp1406I	AACGTT	1 cut(s)	1492
Psp6I	CCWGG	9 cut(s)	91, 235, 306, 427, 433, 902, 1935, 2066, 2305
PspEI	GGTNACC	1 cut(s)	1607
PspFI	CCCAGC	2 cut(s)	451, 2615
PspGI	CCWGG	9 cut(s)	91, 235, 306, 427, 433, 902, 1935, 2066, 2305
PspN4I	GGNNCC	5 cut(s)	234, 674, 1538, 2524, 2623
PspPI	GGNCC	2 cut(s)	431, 580
PspXI	VCTCGAGB	1 cut(s)	2679
PstNI	CAGNNNCTG	1 cut(s)	481
PsuI	RGATCY	4 cut(s)	1216, 1661, 1862, 2269
PvuII	CAGCTG	2 cut(s)	455, 2224
RsaI	GTAC	8 cut(s)	621, 674, 832, 864, 921, 2148, 2173, 2700
RsaNI	GTAC	8 cut(s)	620, 673, 831, 863, 920, 2147, 2172, 2699
RseI	CAYNNNNRTG	3 cut(s)	915, 1722, 2595
SacI	GAGCTC	2 cut(s)	1393, 2686
SapI	GCTCTTC	1 cut(s)	1348
SatI	GCNGC	4 cut(s)	482, 2064, 2225, 2427
Sau96I	GGNCC	2 cut(s)	431, 580
SchI	GAGTC	2 cut(s)	1663, 2006
SduI	GDGCHC	6 cut(s)	1360, 1393, 1539, 2458, 2525, 2686
SexAI	ACCWGGT	1 cut(s)	433
SfcI	CTRYAG	6 cut(s)	723, 1746, 1824, 2175, 2219, 2352
Sfr274I	CTCGAG	2 cut(s)	2505, 2679
SinI	GGWCC	2 cut(s)	431, 580
SlaI	CTCGAG	2 cut(s)	2505, 2679
SmiMI	CAYNNNNRTG	3 cut(s)	915, 1722, 2595
SmlI	CTYRAG	5 cut(s)	135, 592, 1476, 2505, 2679
SmoI	CTYRAG	5 cut(s)	135, 592, 1476, 2505, 2679
SseBI	AGGCCT	1 cut(s)	907
SspMI	CTAG	3 cut(s)	86, 156, 174
SstI	GAGCTC	2 cut(s)	1393, 2686
StuI	AGGCCT	1 cut(s)	907
StyI	CCWWGG	5 cut(s)	520, 583, 952, 1775, 2287
TaaI	ACNGT	9 cut(s)	924, 1130, 1210, 1293, 1334, 2176, 2356, 2394, 2581
TaiI	ACGT	7 cut(s)	211, 1057, 1095, 1158, 1495, 2215, 2329
TaqI	TCGA	7 cut(s)	1010, 1584, 2151, 2444, 2493, 2506, 2680
TatI	WGTACW	6 cut(s)	619, 830, 862, 919, 2146, 2698
TfiI	GAWTC	7 cut(s)	203, 698, 773, 1646, 2047, 2342, 2486
TscAI	CASTG	2 cut(s)	108, 687
TseFI	GTSAC	2 cut(s)	1130, 1405
TseI	GCWGC	4 cut(s)	481, 2063, 2224, 2426
Tsp45I	GTSAC	2 cut(s)	1130, 1405
TspRI	CASTG	2 cut(s)	108, 687
Van91I	CCANNNNNTGG	1 cut(s)	682
VneI	GTGCAC	1 cut(s)	2454
VpaK11BI	GGWCC	2 cut(s)	431, 580
VspI	ATTAAT	1 cut(s)	324
XagI	CCTNNNNNAGG	1 cut(s)	432
XapI	RAATTY	4 cut(s)	600, 973, 1036, 2705
XceI	RCATGY	1 cut(s)	450
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2064
XhoI	CTCGAG	2 cut(s)	2505, 2679
XmiI	GTMKAC	1 cut(s)	1749
XmnI	GAANNNNTTC	4 cut(s)	717, 876, 1512, 1575
XspI	CTAG	3 cut(s)	86, 156, 174
Zsp2I	ATGCAT	2 cut(s)	406, 897

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.