Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0009110

Physical Location & Seq

Genomic Coordinates Reverse (-)

25421198 .. 25429441

Gene Structure 8244 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1175300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2745 bp

ATGAATTTCACTTTGTGTTCGTCATCTTCGGAGCTCAGATATACTTTTGTTGAGGGGTTGCCATGTACAAACAAGAATATGGTGGACTCTGAGCCTTTTGTGAAGGTTGATAAGTTCAATAAGAAGGATAGTACTCGAGCATCTAATCACAATGCTAGAAAGCTGGTGATGTCTAGCAATTTGATGCCTCAGCGCTTAAAAGGTTCCAATATGGGAAGGAAATATCTCGAGGAACCTGATCGGAAGCTGGAATTTGTGAGGACATTGTTGATTGACAACTATGACAGCTACACATACAATATATACCAGGAGCTGTCTGTCATTAATGGGGTGCCTCCTGTGGTCGTGCGGAATGATGAATTGACATGGAAAGACATCCGCTATTACTTGTACGAAGACAATGCTTTTGACAATGTTGTTATATCACCTGGACCTGGTTCGCCAGAATGTCCAGCAGACATAGGAATATGTCTTCAGGTGCTGCTTGAATGCTGGGATATCCCTATTTTGGGCGTTTGCCTTGGACATCAGGCTTTAGGTTATGTGCATGGTGCTAAAGTTGTCCACGCATCTGAACCAGTCCATGGACGGTTGAGTGAAATTGAGCACAACGGGTGCAAACTTTTCAATGATATCCCTTCCGGCCACAACTCAGGATTTAAGGTGGTTCGGTACCATTCACTTGTTGTAGATGCTGAATCCCTCCCAGATGAACTCATTCCTATAGCATGGACTTACTCCAGGGATGCACTTTCTTTTTTTGAAACCCAGAAGTCTGATGTTCCCTCAGAATTTGCTGTGGGATCTTTTCCAACAAAACCGAAAAATGGAAGTTTTTCGCCTTTCAGTTGTTCTGGAAAAGTACGGAGCGAAAAGGTTCTCATGGGCATTGTGCATTCTACCCGGCCTCATTATGGTTTGCAGTTCCATCCAGAGAGTATTGCGACCTTTCATGGGAGGCAGATATTCAAGAATTTTAGAGAGATCACAGAGGACTATTGGCTGAATTCGAGGGCATCATTCATCAAGAAGCGGAATTTTGATTATACAGCGTGCATGCAGATGCCCAACGCTAATCGATTCCTCACAGAAGTTCCTAGACATAGGCAACTAGTAAGTAATTCAGATGGTCAGCTATATAGCAAGATTTCTAGAAGTACCTCATTGAAGAACAGTGCAAGTAACACAAGTTCTTCTCGTGTGGTCAATATGGTAAACCTTTCGCATCCAGGCATTGGTGTTAAATATCTAAAGTTGAGATGGAAGAGATTTAAGCATTTGGCTGGTCAAGTTGGTGGAGCAAAAAATATATTCTGTACACTGTTTGGACATCATAAAGCTGAAAATACCTTTTGGTTGGACAGTTCATCGATAGAAAAGAGAAGAGCACGGTTTTCATTTATGGGAGGAAAAGGTGGAACTCTTTGGAAGCAGTTGACCTATAAATTATCAGATAGAAGTGATACGACTTTGAAGGGCAGTGGTTTTCTATCAGTTGAGGACGCTCAAGGCTCTAACAGAAGCACATTTTTGGAAGAAGGGTTTCTTGATTTTTTGGAAAAGGAGCTCCTGTCATTTCGGTACGATGAAAAGGATTATGAAGGCCTTCCATTTGAATTTCATGGTGGATATATTGGCTACATGGGGTATAGCCTTAAATTTGAATGTGGTGCATCTTCTAACTACCACAAATCCAGGACTCCAGATGCTTGCTTTTTCTTTGCTGATAATCTTGTAGTTATCGATCACTGCTCCGACGATGTTTATATACTGTCTATTAACGAAGAACGCACAAGGACGACACCATGGTTGGACGATACAGAACAGAAGCTTCTCAGGCTAAAAGCTTCTGCTACAAAGGAGGGAGAGGAGCCAACTTTACAGGCTCTTCGATCTTTTCAGTGTCAAGAAAGTTTTGTTGTTGATAAATCAAGAGAGCAGTATATAAAAGATGTCGACAAGTGTCTGGAATACATTAAAGATGGTGAAAGCTATGAGTTATGTCTCACAACTCAGATGAGAAAAAGAATTGGGGAGATGGACTCGTTAGGACTTTACCTTCACCTTAGAGAAAAAAATCCAGCACCATATGCTGCCTGGCTTAATTTTTCAAAGGAAAATCTTTGTGTCTGCTGTTCTTCCCCAGAGCGGTTTTTACGGTTGGATAGAAATGGTATACTAGAAGCAAAGCCCATTAAGGGTACTATAGCTCGTGGTGCAACACTAGAGGAAGACGAACAACGCAAACTACAACTGCAATTCAGTGAAAAGGATCAAGCTGAAAATTTGATGATTGTTGATCTTCTAAGAAATGACCTTGGCCGTGTGTGTGAGCCCGGTTCTGTTCATGTGCCCCATCTTATGGATGTAGAATCATATGCTACTGTCCATACTATGGTGAGCACAATTCGCGGGAAAAAGCGGTCAGATGTAAGTGCAGTTGACTGTGTGAGAGCAGCATTTCCAGGCGGTTCAATGACAGGTGCACCAAAATTAAGATCAATGGAACTTCTTGACTCGATCGAAACTAGTCCACGAGGAATCTACTCAGGATGCATTGGTTTTTTCTCATATAACCAGACTTTCGATCTCAACATAGTTATCAGAACTGTTGTTATACACGAGGGTGAAGCTTCAATAGGCGCTGGAGGAGCCATTGTTGCGTTATCAAATCCTGAAGACGAGTATGACGAAATGATGCTGAAAACGCGCGCCCCGGCAAAAGCTGTAACGGAATTTTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006766 GO:0006767 GO:0006807 GO:0008150 GO:0008152 GO:0008153 GO:0008483 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009108 GO:0009110 GO:0009396 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016769 GO:0017144 GO:0018130 GO:0019438 GO:0019752 GO:0032787 GO:0034641 GO:0042364 GO:0042398 GO:0042537 GO:0042558 GO:0042559 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046417 GO:0046482 GO:0046483 GO:0046653 GO:0046654 GO:0046655 GO:0046656 GO:0046820 GO:0051186 GO:0051188 GO:0071704 GO:0072330 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00790 map00790

Pfam Domains

Protein Families

Anth_synt_I_N Chorismate_bind GATase

Protein Analysis

915

Amino Acids

103.26

Weight (kDa)

6.6

Isoelectric Point (pI)

45.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase	PF00117	89 - 248	2.7e-30	Glutamine amidotransferase class-I
GATase	PF00117	283 - 326	1.1e-07	Glutamine amidotransferase class-I
Anth_synt_I_N	PF04715	446 - 590	1.7e-15	Anthranilate synthase component I, N terminal region
Chorismate_bind	PF00425	645 - 902	3.1e-85	chorismate binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28880
fragaria_vesca	FvH4_7g19690
malus_domestica	MD01G1109300.v1.1 MD07G1175300.v1.1
prunus_persica	Prupe.2G214500_v2.0.a1
pyrus_communis	pycom01g13660 pycom07g16970
rosa_chinensis	RchiOBHm_Chr1g0363521
rosa_laevigata	RLG00000027559
rosa_multiflora	Rmu_ssc0000433.1_g000001
rosa_roxburghii	Rroxscaffold_4G00293090
rosa_rugosa	Rorug01G0308100
rosa_samantha	Rh1AG316800 Rh1BG280200 Rh1CG296300 Rh1DG311400 Rh5DG228600
rosa_wichuraiana	Rw0G000800 Rw1G028080 Rw2G022180 Rw3G029370 Rw5G038670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	672
AccB1I	GGYRCC	2 cut(s)	331, 672
AccB7I	CCANNNNNTGG	4 cut(s)	584, 1235, 2362, 2395
AccBSI	CCGCTC	1 cut(s)	2149
AccI	GTMKAC	2 cut(s)	1956, 2176
AccII	CGCG	3 cut(s)	2412, 2710, 2712
AciI	CCGC	7 cut(s)	349, 379, 1033, 2149, 2412, 2422, 2469
AclWI	GGATC	2 cut(s)	811, 2280
AcoI	YGGCCR	2 cut(s)	643, 2320
AcuI	CTGAAG	2 cut(s)	458, 2697
AdeI	CACNNNGTG	1 cut(s)	15
AfaI	GTAC	9 cut(s)	67, 133, 392, 674, 864, 1159, 1318, 1583, 2203
AfeI	AGCGCT	1 cut(s)	194
AhlI	ACTAGT	2 cut(s)	1111, 2528
AjnI	CCWGG	8 cut(s)	306, 427, 433, 740, 1228, 1694, 2096, 2464
AleI	CACNNNNGTG	1 cut(s)	2625
Alw21I	GWGCWC	6 cut(s)	36, 609, 1390, 1569, 2405, 2488
Alw26I	GTCTC	1 cut(s)	2009
Alw44I	GTGCAC	1 cut(s)	2484
AlwI	GGATC	2 cut(s)	811, 2280
AlwNI	CAGNNNCTG	2 cut(s)	313, 481
Ama87I	CYCGRG	2 cut(s)	135, 227
Aor51HI	AGCGCT	1 cut(s)	194
AoxI	GGCC	4 cut(s)	643, 905, 1603, 2320
ApaLI	GTGCAC	1 cut(s)	2484
ApeKI	GCWGC	3 cut(s)	481, 2093, 2456
ArsI	GACNNNNNNTTYG	2 cut(s)	389, 421
AseI	ATTAAT	1 cut(s)	324
Asp700I	GAANNNNTTC	5 cut(s)	717, 835, 876, 1542, 1605
Asp718I	GGTACC	1 cut(s)	672
AspLEI	GCGC	4 cut(s)	195, 2645, 2712, 2714
AspS9I	GGNCC	1 cut(s)	431
AsuC2I	CCSGG	3 cut(s)	904, 2337, 2717
AsuHPI	GGTGA	6 cut(s)	178, 417, 1997, 2054, 2410, 2639
AvaI	CYCGRG	2 cut(s)	135, 227
AvaII	GGWCC	1 cut(s)	431
BaeGI	GKGCMC	2 cut(s)	2355, 2488
BanI	GGYRCC	2 cut(s)	331, 672
BanII	GRGCYC	3 cut(s)	36, 1569, 2337
BauI	CACGAG	4 cut(s)	1197, 2211, 2535, 2621
BbsI	GAAGAC	4 cut(s)	402, 464, 2238, 2685
Bbv12I	GWGCWC	6 cut(s)	36, 609, 1390, 1569, 2405, 2488
BbvCI	CCTCAGC	1 cut(s)	189
BbvI	GCAGC	3 cut(s)	468, 2080, 2468
BccI	CCATC	6 cut(s)	936, 1121, 1254, 1976, 2032, 2364
BceAI	ACGGC	1 cut(s)	2307
BcgI	CGANNNNNNTGC	2 cut(s)	383, 417
BciT130I	CCWGG	8 cut(s)	308, 429, 435, 742, 1230, 1696, 2098, 2466
BcnI	CCSGG	3 cut(s)	904, 2337, 2717
BcoDI	GTCTC	1 cut(s)	2009
BcuI	ACTAGT	2 cut(s)	1111, 2528
BfaI	CTAG	8 cut(s)	156, 174, 1098, 1112, 1152, 2180, 2225, 2529
BfmI	CTRYAG	2 cut(s)	723, 2205
BfoI	RGCGCY	2 cut(s)	196, 2646
BisI	GCNGC	3 cut(s)	482, 2094, 2457
BlsI	GCNGC	3 cut(s)	483, 2095, 2458
BmcAI	AGTACT	1 cut(s)	133
Bme18I	GGWCC	1 cut(s)	431
BmeT110I	CYCGRG	2 cut(s)	135, 227
BmgT120I	GGNCC	1 cut(s)	431
BmiI	GGNNCC	6 cut(s)	205, 234, 333, 674, 1872, 2653
BoxI	GACNNNNGTC	1 cut(s)	1962
BpiI	GAAGAC	4 cut(s)	402, 464, 2238, 2685
BplI	GAGNNNNNCTC	2 cut(s)	2027, 2059
BpmI	CTGGAG	3 cut(s)	724, 1686, 2667
Bpu10I	CCTNAGC	1 cut(s)	189
BpuEI	CTTGAG	1 cut(s)	1491
BpuMI	CCSGG	3 cut(s)	904, 2337, 2717
Bsa29I	ATCGAT	3 cut(s)	1078, 1370, 1743
BsaJI	CCNNGG	6 cut(s)	520, 583, 741, 1805, 2317, 2715
BsaXI	ACNNNNNCTCC	4 cut(s)	1556, 1586, 2643, 2673
Bse1I	ACTGG	1 cut(s)	578
BseBI	CCWGG	8 cut(s)	308, 429, 435, 742, 1230, 1696, 2098, 2466
BseCI	ATCGAT	3 cut(s)	1078, 1370, 1743
BseDI	CCNNGG	6 cut(s)	520, 583, 741, 1805, 2317, 2715
BseGI	GGATG	6 cut(s)	375, 751, 928, 1225, 2371, 2558
BseMII	CTCAG	8 cut(s)	49, 81, 203, 666, 801, 1849, 2027, 2562
BseNI	ACTGG	1 cut(s)	578
BsePI	GCGCGC	1 cut(s)	2710
BseRI	GAGGAG	2 cut(s)	1883, 2664
BseSI	GKGCMC	2 cut(s)	2355, 2488
BseXI	GCAGC	3 cut(s)	468, 2080, 2468
BseYI	CCCAGC	1 cut(s)	492
BsgI	GTGCAG	1 cut(s)	2457
Bsh1236I	CGCG	3 cut(s)	2412, 2710, 2712
Bsh1285I	CGRYCG	1 cut(s)	2523
BshFI	GGCC	4 cut(s)	645, 907, 1605, 2322
BshNI	GGYRCC	2 cut(s)	331, 672
BshVI	ATCGAT	3 cut(s)	1078, 1370, 1743
BsiEI	CGRYCG	1 cut(s)	2523
BsiHKAI	GWGCWC	6 cut(s)	36, 609, 1390, 1569, 2405, 2488
BsiHKCI	CYCGRG	2 cut(s)	135, 227
BsiSI	CCGG	4 cut(s)	642, 904, 2337, 2717
BsmAI	GTCTC	1 cut(s)	2009
BsmI	GAATGC	2 cut(s)	494, 895
BsnI	GGCC	4 cut(s)	645, 907, 1605, 2322
BsoBI	CYCGRG	2 cut(s)	135, 227
Bsp1286I	GDGCHC	8 cut(s)	36, 609, 1390, 1569, 2337, 2355, 2405, 2488
Bsp1407I	TGTACA	2 cut(s)	65, 1316
Bsp19I	CCATGG	2 cut(s)	583, 1805
BspACI	CCGC	7 cut(s)	349, 379, 1033, 2149, 2412, 2422, 2469
BspANI	GGCC	4 cut(s)	645, 907, 1605, 2322
BspCNI	CTCAG	8 cut(s)	48, 82, 202, 665, 800, 1848, 2026, 2561
BspDI	ATCGAT	3 cut(s)	1078, 1370, 1743
BspFNI	CGCG	3 cut(s)	2412, 2710, 2712
BspLI	GGNNCC	6 cut(s)	205, 234, 333, 674, 1872, 2653
BspPI	GGATC	2 cut(s)	811, 2280
BspQI	GCTCTTC	2 cut(s)	1378, 1893
BspT107I	GGYRCC	2 cut(s)	331, 672
BsrBI	CCGCTC	1 cut(s)	2149
BsrGI	TGTACA	2 cut(s)	65, 1316
BsrI	ACTGG	1 cut(s)	578
BssECI	CCNNGG	6 cut(s)	520, 583, 741, 1805, 2317, 2715
BssHII	GCGCGC	1 cut(s)	2710
BssNAI	GTATAC	1 cut(s)	2177
BssSI	CACGAG	4 cut(s)	1197, 2211, 2535, 2621
BssT1I	CCWWGG	4 cut(s)	520, 583, 1805, 2317
Bst1107I	GTATAC	1 cut(s)	2177
Bst2BI	CACGAG	4 cut(s)	1197, 2211, 2535, 2621
Bst2UI	CCWGG	8 cut(s)	308, 429, 435, 742, 1230, 1696, 2098, 2466
Bst6I	CTCTTC	3 cut(s)	1259, 1378, 1893
BstAPI	GCANNNNNTGC	1 cut(s)	2090
BstAUI	TGTACA	2 cut(s)	65, 1316
BstC8I	GCNNGC	4 cut(s)	1054, 1058, 1711, 2712
BstDSI	CCRYGG	2 cut(s)	583, 1805
BstF5I	GGATG	6 cut(s)	375, 751, 928, 1225, 2371, 2558
BstFNI	CGCG	3 cut(s)	2412, 2710, 2712
BstH2I	RGCGCY	2 cut(s)	196, 2646
BstHHI	GCGC	4 cut(s)	195, 2645, 2712, 2714
BstMAI	GTCTC	1 cut(s)	2009
BstMCI	CGRYCG	1 cut(s)	2523
BstMWI	GCNNNNNNNGC	7 cut(s)	1837, 2090, 2216, 2409, 2651, 2660, 2707
BstNI	CCWGG	8 cut(s)	308, 429, 435, 742, 1230, 1696, 2098, 2466
BstNSI	RCATGY	1 cut(s)	1060
BstPAI	GACNNNNGTC	1 cut(s)	1962
BstSFI	CTRYAG	2 cut(s)	723, 2205
BstSLI	GKGCMC	2 cut(s)	2355, 2488
BstUI	CGCG	3 cut(s)	2412, 2710, 2712
BstV1I	GCAGC	3 cut(s)	468, 2080, 2468
BstV2I	GAAGAC	4 cut(s)	402, 464, 2238, 2685
BstX2I	RGATCY	1 cut(s)	803
BstYI	RGATCY	1 cut(s)	803
BstZ17I	GTATAC	1 cut(s)	2177
Bsu15I	ATCGAT	3 cut(s)	1078, 1370, 1743
BsuRI	GGCC	4 cut(s)	645, 907, 1605, 2322
BsuTUI	ATCGAT	3 cut(s)	1078, 1370, 1743
BtgI	CCRYGG	2 cut(s)	583, 1805
BtsCI	GGATG	6 cut(s)	375, 751, 928, 1225, 2371, 2558
BtsI	GCAGTG	2 cut(s)	1486, 1747
BtsIMutI	CAGTG	6 cut(s)	1180, 1319, 1486, 1747, 1907, 2269
Cac8I	GCNNGC	4 cut(s)	1054, 1058, 1711, 2712
CaiI	CAGNNNCTG	2 cut(s)	313, 481
CfoI	GCGC	4 cut(s)	195, 2645, 2712, 2714
Cfr13I	GGNCC	1 cut(s)	431
ClaI	ATCGAT	3 cut(s)	1078, 1370, 1743
CseI	GACGC	1 cut(s)	1511
CsiI	ACCWGGT	1 cut(s)	433
Csp6I	GTAC	9 cut(s)	66, 132, 391, 673, 863, 1158, 1317, 1582, 2202
CviQI	GTAC	9 cut(s)	66, 132, 391, 673, 863, 1158, 1317, 1582, 2202
DraIII	CACNNNGTG	1 cut(s)	15
EaeI	YGGCCR	2 cut(s)	643, 2320
Eam1104I	CTCTTC	3 cut(s)	1259, 1378, 1893
EarI	CTCTTC	3 cut(s)	1259, 1378, 1893
Ecl136II	GAGCTC	2 cut(s)	34, 1567
Eco130I	CCWWGG	4 cut(s)	520, 583, 1805, 2317
Eco147I	AGGCCT	1 cut(s)	1605
Eco24I	GRGCYC	3 cut(s)	36, 1569, 2337
Eco32I	GATATC	2 cut(s)	499, 634
Eco47I	GGWCC	1 cut(s)	431
Eco47III	AGCGCT	1 cut(s)	194
Eco53kI	GAGCTC	2 cut(s)	34, 1567
Eco57I	CTGAAG	2 cut(s)	458, 2697
Eco88I	CYCGRG	2 cut(s)	135, 227
EcoICRI	GAGCTC	2 cut(s)	34, 1567
EcoRI	GAATTC	1 cut(s)	1006
EcoRII	CCWGG	8 cut(s)	306, 427, 433, 740, 1228, 1694, 2096, 2464
EcoRV	GATATC	2 cut(s)	499, 634
EcoT14I	CCWWGG	4 cut(s)	520, 583, 1805, 2317
EcoT22I	ATGCAT	1 cut(s)	2558
EcoT38I	GRGCYC	3 cut(s)	36, 1569, 2337
ErhI	CCWWGG	4 cut(s)	520, 583, 1805, 2317
FalI	AAGNNNNNCTT	2 cut(s)	1530, 1562
FauI	CCCGC	1 cut(s)	2405
FauNDI	CATATG	2 cut(s)	2089, 2377
FblI	GTMKAC	2 cut(s)	1956, 2176
Fnu4HI	GCNGC	3 cut(s)	482, 2094, 2457
FokI	GGATG	6 cut(s)	362, 758, 915, 1212, 2378, 2565
FriOI	GRGCYC	3 cut(s)	36, 1569, 2337
Fsp4HI	GCNGC	3 cut(s)	482, 2094, 2457
FspBI	CTAG	8 cut(s)	156, 174, 1098, 1112, 1152, 2180, 2225, 2529
GlaI	GCGC	4 cut(s)	194, 2644, 2711, 2713
GluI	GCNGC	3 cut(s)	482, 2094, 2457
GsaI	CCCAGC	1 cut(s)	496
GsuI	CTGGAG	3 cut(s)	724, 1686, 2667
HaeII	RGCGCY	2 cut(s)	196, 2646
HaeIII	GGCC	4 cut(s)	645, 907, 1605, 2322
HapII	CCGG	4 cut(s)	642, 904, 2337, 2717
HgaI	GACGC	1 cut(s)	1511
HhaI	GCGC	4 cut(s)	195, 2645, 2712, 2714
Hin6I	GCGC	4 cut(s)	193, 2643, 2710, 2712
HinP1I	GCGC	4 cut(s)	193, 2643, 2710, 2712
HincII	GTYRAC	3 cut(s)	1437, 1957, 2443
HindII	GTYRAC	3 cut(s)	1437, 1957, 2443
HindIII	AAGCTT	3 cut(s)	1829, 1845, 2631
HinfI	GANTC	8 cut(s)	86, 698, 1080, 1699, 2042, 2372, 2516, 2541
HpaII	CCGG	4 cut(s)	642, 904, 2337, 2717
HphI	GGTGA	6 cut(s)	178, 417, 1997, 2054, 2410, 2639
Hpy99I	CGWCG	1 cut(s)	1760
HpyAV	CCTTC	9 cut(s)	97, 118, 210, 648, 1468, 1532, 1595, 1616, 2069
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1837, 2090, 2216, 2409, 2651, 2660, 2707
HspAI	GCGC	4 cut(s)	193, 2643, 2710, 2712
KpnI	GGTACC	1 cut(s)	676
LguI	GCTCTTC	2 cut(s)	1378, 1893
LmnI	GCTCC	9 cut(s)	31, 310, 867, 1298, 1564, 1572, 1757, 1870, 2651
Lsp1109I	GCAGC	3 cut(s)	468, 2080, 2468
MabI	ACCWGGT	1 cut(s)	433
MaeI	CTAG	8 cut(s)	156, 174, 1098, 1112, 1152, 2180, 2225, 2529
MaeIII	GTNAC	2 cut(s)	1181, 2728
MbiI	CCGCTC	1 cut(s)	2149
MflI	RGATCY	1 cut(s)	803
MhlI	GDGCHC	8 cut(s)	36, 609, 1390, 1569, 2337, 2355, 2405, 2488
MlyI	GAGTC	4 cut(s)	80, 1693, 2036, 2510
MmeI	TCCRAC	5 cut(s)	836, 1338, 1779, 1791, 2142
Mph1103I	ATGCAT	1 cut(s)	2558
MroXI	GAANNNNTTC	5 cut(s)	717, 835, 876, 1542, 1605
MslI	CAYNNNNRTG	2 cut(s)	1061, 2625
MspI	CCGG	4 cut(s)	642, 904, 2337, 2717
Mva1269I	GAATGC	2 cut(s)	494, 895
MvaI	CCWGG	8 cut(s)	308, 429, 435, 742, 1230, 1696, 2098, 2466
MvnI	CGCG	3 cut(s)	2412, 2710, 2712
MwoI	GCNNNNNNNGC	7 cut(s)	1837, 2090, 2216, 2409, 2651, 2660, 2707
NciI	CCSGG	3 cut(s)	904, 2337, 2717
NcoI	CCATGG	2 cut(s)	583, 1805
NdeI	CATATG	2 cut(s)	2089, 2377
NlaIV	GGNNCC	6 cut(s)	205, 234, 333, 674, 1872, 2653
NsiI	ATGCAT	1 cut(s)	2558
NspI	RCATGY	1 cut(s)	1060
OliI	CACNNNNGTG	1 cut(s)	2625
PaeI	GCATGC	1 cut(s)	1060
PaeR7I	CTCGAG	2 cut(s)	135, 227
PauI	GCGCGC	1 cut(s)	2710
PceI	AGGCCT	1 cut(s)	1605
PciSI	GCTCTTC	2 cut(s)	1378, 1893
PcsI	WCGNNNNNNNCGW	1 cut(s)	2688
PctI	GAATGC	2 cut(s)	494, 895
PdmI	GAANNNNTTC	5 cut(s)	717, 835, 876, 1542, 1605
PfeI	GAWTC	4 cut(s)	698, 1080, 2372, 2541
PflMI	CCANNNNNTGG	4 cut(s)	584, 1235, 2362, 2395
PfoI	TCCNGGA	1 cut(s)	1694
PkrI	GCNGC	3 cut(s)	483, 2095, 2458
Ple19I	CGATCG	1 cut(s)	2523
PleI	GAGTC	4 cut(s)	80, 1693, 2036, 2510
PpsI	GAGTC	4 cut(s)	80, 1693, 2036, 2510
PshAI	GACNNNNGTC	1 cut(s)	1962
PshBI	ATTAAT	1 cut(s)	324
Psp124BI	GAGCTC	2 cut(s)	36, 1569
Psp6I	CCWGG	8 cut(s)	306, 427, 433, 740, 1228, 1694, 2096, 2464
PspFI	CCCAGC	1 cut(s)	492
PspGI	CCWGG	8 cut(s)	306, 427, 433, 740, 1228, 1694, 2096, 2464
PspN4I	GGNNCC	6 cut(s)	205, 234, 333, 674, 1872, 2653
PspPI	GGNCC	1 cut(s)	431
PspXI	VCTCGAGB	1 cut(s)	135
PsrI	GAACNNNNNNTAC	2 cut(s)	1174, 1206
PstNI	CAGNNNCTG	2 cut(s)	313, 481
PsuI	RGATCY	1 cut(s)	803
PteI	GCGCGC	1 cut(s)	2710
PvuI	CGATCG	1 cut(s)	2523
RsaI	GTAC	9 cut(s)	67, 133, 392, 674, 864, 1159, 1318, 1583, 2203
RsaNI	GTAC	9 cut(s)	66, 132, 391, 673, 863, 1158, 1317, 1582, 2202
RseI	CAYNNNNRTG	2 cut(s)	1061, 2625
SacI	GAGCTC	2 cut(s)	36, 1569
SalI	GTCGAC	1 cut(s)	1955
SapI	GCTCTTC	2 cut(s)	1378, 1893
SatI	GCNGC	3 cut(s)	482, 2094, 2457
Sau96I	GGNCC	1 cut(s)	431
ScaI	AGTACT	1 cut(s)	133
SchI	GAGTC	4 cut(s)	80, 1693, 2036, 2510
SduI	GDGCHC	8 cut(s)	36, 609, 1390, 1569, 2337, 2355, 2405, 2488
SexAI	ACCWGGT	1 cut(s)	433
SfcI	CTRYAG	2 cut(s)	723, 2205
Sfr274I	CTCGAG	2 cut(s)	135, 227
SinI	GGWCC	1 cut(s)	431
SlaI	CTCGAG	2 cut(s)	135, 227
SmiMI	CAYNNNNRTG	2 cut(s)	1061, 2625
SmlI	CTYRAG	3 cut(s)	135, 227, 1506
SmoI	CTYRAG	3 cut(s)	135, 227, 1506
SpeI	ACTAGT	2 cut(s)	1111, 2528
SphI	GCATGC	1 cut(s)	1060
SseBI	AGGCCT	1 cut(s)	1605
SsiI	CCGC	7 cut(s)	349, 379, 1033, 2149, 2412, 2422, 2469
SspMI	CTAG	8 cut(s)	156, 174, 1098, 1112, 1152, 2180, 2225, 2529
SstI	GAGCTC	2 cut(s)	36, 1569
StuI	AGGCCT	1 cut(s)	1605
StyI	CCWWGG	4 cut(s)	520, 583, 1805, 2317
TatI	WGTACW	3 cut(s)	65, 131, 1316
TfiI	GAWTC	4 cut(s)	698, 1080, 2372, 2541
TscAI	CASTG	6 cut(s)	1180, 1326, 1486, 1754, 1907, 2269
TseI	GCWGC	3 cut(s)	481, 2093, 2456
TspGWI	ACGGA	1 cut(s)	880
TspRI	CASTG	6 cut(s)	1180, 1326, 1486, 1754, 1907, 2269
Van91I	CCANNNNNTGG	4 cut(s)	584, 1235, 2362, 2395
VneI	GTGCAC	1 cut(s)	2484
VpaK11BI	GGWCC	1 cut(s)	431
VspI	ATTAAT	1 cut(s)	324
XbaI	TCTAGA	1 cut(s)	1151
XceI	RCATGY	1 cut(s)	1060
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2094
XhoI	CTCGAG	2 cut(s)	135, 227
XmiI	GTMKAC	2 cut(s)	1956, 2176
XmnI	GAANNNNTTC	5 cut(s)	717, 835, 876, 1542, 1605
XspI	CTAG	8 cut(s)	156, 174, 1098, 1112, 1152, 2180, 2225, 2529
ZrmI	AGTACT	1 cut(s)	133
Zsp2I	ATGCAT	1 cut(s)	2558

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.