Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1C

Orthogroup ID

OG0009110

Physical Location & Seq

Genomic Coordinates Reverse (-)

56953207 .. 56960462

Gene Structure 7256 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1CG296300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2643 bp

ATGAAGTTCACCTTGTGTTCATCATCTTCTGAGCTTAGATATCCTGTTGTTGAGGGGTTGCCCAGACGTACAAACAAGAATGTACTAGAGCCTGAACCGTTGGTGAAGGTTGATAATTTTGTTAAGAAGGTTAATGCTCAAGCATCTAATCATGATACGAGGAAGCTGGTGATGTCTAGCAATTTGATGCCTCAGCACATACAAGAATCACATGTGGGAAAGAAACAACTGGAGGCACCTGGTCGGAAGTTGGAATTTGTGAGGACATTATTGATTGACAACTATGATAGCTACACATACAATATATTCCAGGAGCTGTCTGTCATTAATGGCTTACCTCCTGTTGTGGTGCGAAATGATGATTTGACATGGAAAGATGTTTGCCATTACTTGTATGAAGAAAATGCATTTGATAACATTGTTATATCACCTGGACCTGGTTCTCCTACATGCCCAGCGGACATAGGTTATGTGCATGGTGCTAAAGTTGTCCACGCATCTGAACCAGTCCATGGACGCTTGAGTGAAATAGAGCACAATGGGTGCATACTTTTCAATGACATTCCCTCTGGCCACAACTCTGGATTCAAGGTGGTTCGGTACCATTCACTGGTCATAGATGCTGAATCACTCCCAGATGAACTCATTCCTATAGCATGGACCTCTTCTGTGAATGCACTTTCTTTTATTGAGACCAAAGAATCTGATGTTGCCTCTAAATTTGCTGATGGGTCTTTTTCAAGGAAACCAAAAAATGGAAGTTATTCACCTTCCAGCCATTCTGGCAAAGTACAGAGTGAAAGGGTTCTCATGGGCATTATGCATTCTTCCAGGCCCCATTATGGTGTACAGTTCCATCCAGAGAGTGTTGCTACCTGCCATGGAAGGCAAATATTCAAGAATTTTAGAAAAATCACAGAGGATTATTGGCTGAGATCAAGGGCATCATTCATCAACGCGCGAAATTTTCCTTTTAATGCACGCATGCAGATGCCCCATGTTGGTCGACTATTGACAGACGTTCCAAAACACAGGCAACCACTAAATAATGCAGATGGTCAATTATATAAAAATGTAAATAACAAAAGTTTCTCTGGCATGGTTGATATGGTAAACCTTTTGCATCCAAGCATTACTATTAAAGATCTGAGGTTGAAATGGAAGAAATTTAAGCACTTGGCCGGTCAAGTTGGTGGAGCAAGAAATATATTTTGTGAACTGTTTGGACAAGATAAAGCTGAAAACACCTTTTGGTTAGACAGTTCATCAATAGAGAAGAGAAGAGCACGCTTTTCATTTATGGGAGGAAAAGGTGGAGCCCTTTGGAAGCAAGTGACCTTTAAATTGTCTGATAAAAGTGATATGGCTTTGAAGGGCAGTGGTCTTCTATCTGTTCAGGATGCTCAAGGCTCTATCAAATGTTCATTTTTGGAAGAAGGTTTTCTTGATTTTCTGAAAAAGGAGCTCCTGTCATTTTGTTATGATGAAAAGGATTATGAAGAACTTCCATTTGATTTTCATGGTGGATATATTGGTTACCTCGGGTATAACCTAAAAGTTGAATGTGGTGTGGATTCTAATCGCCACAGATCCGAGACTCCAGATGCTTGCTTTTTCTTTGCTGATAACCTTGTAGTTGTTGATCATCGTAATGATGATGTTTACATACTGTCTGTAGACGAAGTATGCAGACCTTTGACACCATGGTTGGATGATACAGAACAAAAGCTTCTCAACTTAAAAGCTTCTGCTACAAGGGAGGGAAAGAAGCCAACTTTGCTGGCTCCACAATCTTCACCGTGTCAAGGAAGTTTCCTTGCTGATAAATCAAGAGAAGCGTATATAGAAGATGTTGACAAGTGCCTGGAATACATTAAAGATGGTGAAAGTTATGAATTATGTCTGACAACTCAGATGAGAAAAAGAATTGGGGACATAGACTCATTGGGACTATACCTTCATTTAAGAGAGAAGAATCCAGCACCATATGCTGCCTGGCTTAATTTTTCAGATGAAGACCTATGTATCTGCTGTTCTTCCCCTGAGCGTTTCTTACAATTGGATAGAAATGGTGTACTCGAAGCAAAGCCCATTAAGGGTACCGTAGCTCGTGGTGCGACACCAGAGGAAGATGAACAACGTAAACTACAACTGCGGTATAGTGAAAAGGATCAAGCTGAAAATCTGATGATAGTTGATCTTCTAAGGAATGACCTTGGTCGTGTGTGTGAGCCTGGCTCAGTTCATGTGCCACGTCTTATGGATGTGGAATCATATGCTACGGTTCATACTATGGTCAGCACAATTCGCGGGCAAAAGCGGTCTGATGTGACTGCAATTGATTGTGTGAGAGCAGCATTTCCGGGTGGTTCGATGACTGGTGCACCGAAATTGAGATCAATGGAACTTCTGGATTCAATCGAAACTAGTTCTCGAGGTATCTACTCGGGCTCCATTGGCTTTTTCTCATATAACCAGACATTTGATCTAAATATAGTTATTAGAACTGTTGTTATACACGAGGGTGAAGCTTCAATAGGAGCGGGGGGAGCCATTGTTGCCCTATCAAATCCTGAAGACGAGTACGAGGAGATGGTTTTGAAAACACGAGCTCCAGCAAAAGCGGTACTAGAATTTTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006766 GO:0006767 GO:0006807 GO:0008150 GO:0008152 GO:0008153 GO:0008483 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009108 GO:0009110 GO:0009396 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016769 GO:0017144 GO:0018130 GO:0019438 GO:0019752 GO:0032787 GO:0034641 GO:0042364 GO:0042398 GO:0042537 GO:0042558 GO:0042559 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046417 GO:0046482 GO:0046483 GO:0046653 GO:0046654 GO:0046655 GO:0046656 GO:0046820 GO:0051186 GO:0051188 GO:0071704 GO:0072330 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00790 map00790

Pfam Domains

Protein Families

Anth_synt_I_N Chorismate_bind GATase

Protein Analysis

880

Amino Acids

98.74

Weight (kDa)

6.26

Isoelectric Point (pI)

44.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase	PF00117	90 - 154	3.9e-07	Glutamine amidotransferase class-I
GATase	PF00117	265 - 302	4.2e-08	Glutamine amidotransferase class-I
Anth_synt_I_N	PF04715	412 - 556	7.7e-16	Anthranilate synthase component I, N terminal region
Chorismate_bind	PF00425	611 - 868	4.2e-86	chorismate binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28880
fragaria_vesca	FvH4_7g19690
malus_domestica	MD01G1109300.v1.1 MD07G1175300.v1.1
prunus_persica	Prupe.2G214500_v2.0.a1
pyrus_communis	pycom01g13660 pycom07g16970
rosa_chinensis	RchiOBHm_Chr1g0363521
rosa_laevigata	RLG00000027559
rosa_multiflora	Rmu_ssc0000433.1_g000001
rosa_roxburghii	Rroxscaffold_4G00293090
rosa_rugosa	Rorug01G0308100
rosa_samantha	Rh1AG316800 Rh1BG280200 Rh1CG296300 Rh1DG311400 Rh5DG228600
rosa_wichuraiana	Rw0G000800 Rw1G028080 Rw2G022180 Rw3G029370 Rw5G038670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	884
Acc65I	GGTACC	2 cut(s)	600, 2099
AccB1I	GGYRCC	3 cut(s)	235, 600, 2099
AccB7I	CCANNNNNTGG	3 cut(s)	512, 610, 755
AccBSI	CCGCTC	1 cut(s)	2543
AccI	GTMKAC	2 cut(s)	1006, 1677
AccII	CGCG	3 cut(s)	959, 961, 2310
AciI	CCGC	6 cut(s)	458, 2155, 2310, 2320, 2543, 2624
AclWI	GGATC	2 cut(s)	1584, 2178
AcoI	YGGCCR	2 cut(s)	571, 1179
AcsI	RAATTY	6 cut(s)	254, 719, 901, 964, 1166, 2633
AcuI	CTGAAG	1 cut(s)	2595
AdeI	CACNNNGTG	1 cut(s)	15
AfaI	GTAC	9 cut(s)	70, 84, 602, 792, 849, 2076, 2101, 2585, 2628
AfiI	CCNNNNNNNGG	8 cut(s)	437, 512, 610, 755, 842, 1001, 1805, 2096
AflIII	ACRYGT	1 cut(s)	211
AhlI	ACTAGT	1 cut(s)	2426
AjiI	CACGTC	1 cut(s)	2255
AjnI	CCWGG	8 cut(s)	238, 309, 430, 436, 830, 1863, 1994, 2233
AleI	CACNNNNGTG	1 cut(s)	2523
Alw21I	GWGCWC	5 cut(s)	537, 1288, 1467, 2386, 2614
Alw26I	GTCTC	2 cut(s)	686, 1589
Alw44I	GTGCAC	1 cut(s)	2382
AlwI	GGATC	2 cut(s)	1584, 2178
AlwNI	CAGNNNCTG	1 cut(s)	316
Ama87I	CYCGRG	3 cut(s)	1541, 2433, 2446
AoxI	GGCC	3 cut(s)	571, 833, 1179
ApaLI	GTGCAC	1 cut(s)	2382
ApeKI	GCWGC	2 cut(s)	1991, 2354
ApoI	RAATTY	6 cut(s)	254, 719, 901, 964, 1166, 2633
AseI	ATTAAT	1 cut(s)	327
Asp700I	GAANNNNTTC	5 cut(s)	645, 763, 804, 1440, 1503
Asp718I	GGTACC	2 cut(s)	600, 2099
AspLEI	GCGC	1 cut(s)	961
AspS9I	GGNCC	3 cut(s)	434, 660, 834
AsuC2I	CCSGG	1 cut(s)	2364
AsuHPI	GGTGA	7 cut(s)	115, 181, 420, 759, 1788, 1895, 2537
AvaI	CYCGRG	3 cut(s)	1541, 2433, 2446
AvaII	GGWCC	2 cut(s)	434, 660
BaeGI	GKGCMC	1 cut(s)	2386
BaeI	ACNNNNGTAYC	2 cut(s)	2008, 2041
BalI	TGGCCA	1 cut(s)	573
BanI	GGYRCC	3 cut(s)	235, 600, 2099
BanII	GRGCYC	4 cut(s)	1321, 1467, 2453, 2614
BauI	CACGAG	3 cut(s)	2109, 2519, 2607
BbsI	GAAGAC	3 cut(s)	1376, 2022, 2583
Bbv12I	GWGCWC	5 cut(s)	537, 1288, 1467, 2386, 2614
BbvCI	CCTCAGC	1 cut(s)	192
BbvI	GCAGC	2 cut(s)	1978, 2366
BccI	CCATC	5 cut(s)	722, 864, 1049, 1874, 2587
BciT130I	CCWGG	8 cut(s)	240, 311, 432, 438, 832, 1865, 1996, 2235
BclI	TGATCA	1 cut(s)	1642
BcnI	CCSGG	1 cut(s)	2364
BcoDI	GTCTC	2 cut(s)	686, 1589
BcuI	ACTAGT	1 cut(s)	2426
BfaI	CTAG	4 cut(s)	86, 177, 2427, 2630
BfmI	CTRYAG	2 cut(s)	651, 1674
BfuAI	ACCTGC	1 cut(s)	884
BglI	GCCNNNNNGGC	1 cut(s)	783
BglII	AGATCT	1 cut(s)	1144
BisI	GCNGC	2 cut(s)	1992, 2355
BlsI	GCNGC	2 cut(s)	1993, 2356
Bme1390I	CCNGG	9 cut(s)	240, 311, 432, 438, 832, 1865, 1996, 2235, 2364
Bme18I	GGWCC	2 cut(s)	434, 660
BmeT110I	CYCGRG	3 cut(s)	1541, 2433, 2446
BmgBI	CACGTC	1 cut(s)	2255
BmgT120I	GGNCC	3 cut(s)	434, 660, 834
BmiI	GGNNCC	8 cut(s)	237, 602, 836, 1318, 1785, 2101, 2452, 2551
BmrFI	CCNGG	9 cut(s)	240, 311, 432, 438, 832, 1865, 1996, 2235, 2364
BmsI	GCATC	9 cut(s)	152, 177, 506, 610, 953, 981, 1132, 1390, 1594
BoxI	GACNNNNGTC	1 cut(s)	2217
BpiI	GAAGAC	3 cut(s)	1376, 2022, 2583
BplI	GAGNNNNNCTC	2 cut(s)	2222, 2254
BpmI	CTGGAG	3 cut(s)	251, 1584, 2598
Bpu10I	CCTNAGC	2 cut(s)	192, 2043
BpuEI	CTTGAG	3 cut(s)	123, 541, 1389
BpuMI	CCSGG	1 cut(s)	2364
BsaBI	GATNNNNATC	1 cut(s)	1578
BsaI	GGTCTC	1 cut(s)	686
BsaJI	CCNNGG	5 cut(s)	511, 880, 1540, 1703, 2215
BsaXI	ACNNNNNCTCC	8 cut(s)	1454, 1484, 2435, 2465, 2541, 2571, 2596, 2626
Bsc4I	CCNNNNNNNGG	8 cut(s)	437, 512, 610, 755, 842, 1001, 1805, 2096
Bse118I	RCCGGY	1 cut(s)	1181
Bse1I	ACTGG	4 cut(s)	234, 506, 615, 2383
Bse8I	GATNNNNATC	1 cut(s)	1578
BseBI	CCWGG	8 cut(s)	240, 311, 432, 438, 832, 1865, 1996, 2235
BseDI	CCNNGG	5 cut(s)	511, 880, 1540, 1703, 2215
BseGI	GGATG	5 cut(s)	856, 1123, 1405, 1717, 2269
BseJI	GATNNNNATC	1 cut(s)	1578
BseLI	CCNNNNNNNGG	8 cut(s)	437, 512, 610, 755, 842, 1001, 1805, 2096
BseMII	CTCAG	7 cut(s)	21, 206, 923, 1139, 1925, 2034, 2253
BseNI	ACTGG	4 cut(s)	234, 506, 615, 2383
BseRI	GAGGAG	1 cut(s)	2603
BseSI	GKGCMC	1 cut(s)	2386
BseXI	GCAGC	2 cut(s)	1978, 2366
BseYI	CCCAGC	1 cut(s)	454
Bsh1236I	CGCG	3 cut(s)	959, 961, 2310
BshFI	GGCC	3 cut(s)	573, 835, 1181
BshNI	GGYRCC	3 cut(s)	235, 600, 2099
BsiHKAI	GWGCWC	5 cut(s)	537, 1288, 1467, 2386, 2614
BsiHKCI	CYCGRG	3 cut(s)	1541, 2433, 2446
BsiSI	CCGG	2 cut(s)	1182, 2363
BslFI	GGGAC	2 cut(s)	1946, 1962
BslI	CCNNNNNNNGG	8 cut(s)	437, 512, 610, 755, 842, 1001, 1805, 2096
BsmAI	GTCTC	2 cut(s)	686, 1589
BsmFI	GGGAC	2 cut(s)	1946, 1962
BsmI	GAATGC	2 cut(s)	679, 823
BsnI	GGCC	3 cut(s)	573, 835, 1181
Bso31I	GGTCTC	1 cut(s)	686
BsoBI	CYCGRG	3 cut(s)	1541, 2433, 2446
Bsp1286I	GDGCHC	7 cut(s)	537, 1288, 1321, 1467, 2386, 2453, 2614
Bsp1407I	TGTACA	1 cut(s)	847
Bsp143I	GATC	8 cut(s)	935, 1144, 1589, 1642, 2170, 2197, 2396, 2485
Bsp19I	CCATGG	3 cut(s)	511, 880, 1703
BspACI	CCGC	6 cut(s)	458, 2155, 2310, 2320, 2543, 2624
BspANI	GGCC	3 cut(s)	573, 835, 1181
BspCNI	CTCAG	7 cut(s)	22, 205, 924, 1140, 1924, 2035, 2252
BspFNI	CGCG	3 cut(s)	959, 961, 2310
BspHI	TCATGA	1 cut(s)	151
BspLI	GGNNCC	8 cut(s)	237, 602, 836, 1318, 1785, 2101, 2452, 2551
BspMI	ACCTGC	1 cut(s)	884
BspPI	GGATC	2 cut(s)	1584, 2178
BspQI	GCTCTTC	1 cut(s)	1276
BspT107I	GGYRCC	3 cut(s)	235, 600, 2099
BspTNI	GGTCTC	1 cut(s)	686
BsrBI	CCGCTC	1 cut(s)	2543
BsrFI	RCCGGY	1 cut(s)	1181
BsrGI	TGTACA	1 cut(s)	847
BsrI	ACTGG	4 cut(s)	234, 506, 615, 2383
BssAI	RCCGGY	1 cut(s)	1181
BssECI	CCNNGG	5 cut(s)	511, 880, 1540, 1703, 2215
BssMI	GATC	8 cut(s)	935, 1144, 1589, 1642, 2170, 2197, 2396, 2485
BssSI	CACGAG	3 cut(s)	2109, 2519, 2607
BssT1I	CCWWGG	4 cut(s)	511, 880, 1703, 2215
Bst2BI	CACGAG	3 cut(s)	2109, 2519, 2607
Bst2UI	CCWGG	8 cut(s)	240, 311, 432, 438, 832, 1865, 1996, 2235
Bst4CI	ACNGT	9 cut(s)	99, 852, 1221, 1262, 1671, 1800, 2104, 2284, 2509
Bst6I	CTCTTC	3 cut(s)	670, 1271, 1276
BstAPI	GCANNNNNTGC	1 cut(s)	1988
BstAUI	TGTACA	1 cut(s)	847
BstC8I	GCNNGC	6 cut(s)	982, 986, 1288, 1609, 1782, 2312
BstDEI	CTNAG	9 cut(s)	30, 35, 192, 932, 1148, 1911, 2043, 2204, 2239
BstDSI	CCRYGG	3 cut(s)	511, 880, 1703
BstEII	GGTNACC	1 cut(s)	1535
BstF5I	GGATG	5 cut(s)	856, 1123, 1405, 1717, 2269
BstFNI	CGCG	3 cut(s)	959, 961, 2310
BstHHI	GCGC	1 cut(s)	961
BstKTI	GATC	8 cut(s)	938, 1147, 1592, 1645, 2173, 2200, 2399, 2488
BstMAI	GTCTC	2 cut(s)	686, 1589
BstMBI	GATC	8 cut(s)	935, 1144, 1589, 1642, 2170, 2197, 2396, 2485
BstMWI	GCNNNNNNNGC	8 cut(s)	783, 1777, 1988, 2114, 2307, 2457, 2549, 2558
BstNI	CCWGG	8 cut(s)	240, 311, 432, 438, 832, 1865, 1996, 2235
BstNSI	RCATGY	3 cut(s)	215, 453, 988
BstPAI	GACNNNNGTC	1 cut(s)	2217
BstPI	GGTNACC	1 cut(s)	1535
BstSCI	CCNGG	9 cut(s)	238, 309, 430, 436, 830, 1863, 1994, 2233, 2362
BstSFI	CTRYAG	2 cut(s)	651, 1674
BstSLI	GKGCMC	1 cut(s)	2386
BstUI	CGCG	3 cut(s)	959, 961, 2310
BstV1I	GCAGC	2 cut(s)	1978, 2366
BstV2I	GAAGAC	3 cut(s)	1376, 2022, 2583
BstX2I	RGATCY	2 cut(s)	1144, 1589
BstXI	CCANNNNNNTGG	1 cut(s)	581
BstYI	RGATCY	2 cut(s)	1144, 1589
BsuRI	GGCC	3 cut(s)	573, 835, 1181
BtgI	CCRYGG	3 cut(s)	511, 880, 1703
BtrI	CACGTC	1 cut(s)	2255
BtsCI	GGATG	5 cut(s)	856, 1123, 1405, 1717, 2269
BtsI	GCAGTG	1 cut(s)	1384
BtsIMutI	CAGTG	2 cut(s)	608, 1384
BveI	ACCTGC	1 cut(s)	884
Cac8I	GCNNGC	6 cut(s)	982, 986, 1288, 1609, 1782, 2312
CaiI	CAGNNNCTG	1 cut(s)	316
CciI	TCATGA	1 cut(s)	151
CfoI	GCGC	1 cut(s)	961
Cfr10I	RCCGGY	1 cut(s)	1181
Cfr13I	GGNCC	3 cut(s)	434, 660, 834
CseI	GACGC	1 cut(s)	525
CsiI	ACCWGGT	2 cut(s)	238, 436
Csp6I	GTAC	9 cut(s)	69, 83, 601, 791, 848, 2075, 2100, 2584, 2627
CviQI	GTAC	9 cut(s)	69, 83, 601, 791, 848, 2075, 2100, 2584, 2627
DdeI	CTNAG	9 cut(s)	30, 35, 192, 932, 1148, 1911, 2043, 2204, 2239
DpnI	GATC	8 cut(s)	937, 1146, 1591, 1644, 2172, 2199, 2398, 2487
DpnII	GATC	8 cut(s)	935, 1144, 1589, 1642, 2170, 2197, 2396, 2485
DraI	TTTAAA	1 cut(s)	1342
DraIII	CACNNNGTG	1 cut(s)	15
EaeI	YGGCCR	2 cut(s)	571, 1179
Eam1104I	CTCTTC	3 cut(s)	670, 1271, 1276
EarI	CTCTTC	3 cut(s)	670, 1271, 1276
Ecl136II	GAGCTC	2 cut(s)	1465, 2612
Eco130I	CCWWGG	4 cut(s)	511, 880, 1703, 2215
Eco24I	GRGCYC	4 cut(s)	1321, 1467, 2453, 2614
Eco31I	GGTCTC	1 cut(s)	686
Eco32I	GATATC	1 cut(s)	41
Eco47I	GGWCC	2 cut(s)	434, 660
Eco53kI	GAGCTC	2 cut(s)	1465, 2612
Eco57I	CTGAAG	1 cut(s)	2595
Eco88I	CYCGRG	3 cut(s)	1541, 2433, 2446
Eco91I	GGTNACC	1 cut(s)	1535
EcoICRI	GAGCTC	2 cut(s)	1465, 2612
EcoO109I	RGGNCCY	1 cut(s)	834
EcoO65I	GGTNACC	1 cut(s)	1535
EcoRII	CCWGG	8 cut(s)	238, 309, 430, 436, 830, 1863, 1994, 2233
EcoRV	GATATC	1 cut(s)	41
EcoT14I	CCWWGG	4 cut(s)	511, 880, 1703, 2215
EcoT22I	ATGCAT	2 cut(s)	409, 825
EcoT38I	GRGCYC	4 cut(s)	1321, 1467, 2453, 2614
ErhI	CCWWGG	4 cut(s)	511, 880, 1703, 2215
FalI	AAGNNNNNCTT	3 cut(s)	28, 1428, 1460
FaqI	GGGAC	2 cut(s)	1946, 1962
FauI	CCCGC	2 cut(s)	2303, 2536
FauNDI	CATATG	2 cut(s)	1987, 2275
FbaI	TGATCA	1 cut(s)	1642
FblI	GTMKAC	2 cut(s)	1006, 1677
Fnu4HI	GCNGC	2 cut(s)	1992, 2355
FokI	GGATG	5 cut(s)	843, 1110, 1412, 1724, 2276
FriOI	GRGCYC	4 cut(s)	1321, 1467, 2453, 2614
Fsp4HI	GCNGC	2 cut(s)	1992, 2355
FspBI	CTAG	4 cut(s)	86, 177, 2427, 2630
GlaI	GCGC	1 cut(s)	960
GluI	GCNGC	2 cut(s)	1992, 2355
GsaI	CCCAGC	1 cut(s)	458
GsuI	CTGGAG	3 cut(s)	251, 1584, 2598
HaeIII	GGCC	3 cut(s)	573, 835, 1181
HapII	CCGG	2 cut(s)	1182, 2363
HgaI	GACGC	1 cut(s)	525
HhaI	GCGC	1 cut(s)	961
Hin6I	GCGC	1 cut(s)	959
HinP1I	GCGC	1 cut(s)	959
HincII	GTYRAC	2 cut(s)	1007, 1855
HindII	GTYRAC	2 cut(s)	1007, 1855
HindIII	AAGCTT	3 cut(s)	1727, 1743, 2529
HpaII	CCGG	2 cut(s)	1182, 2363
HphI	GGTGA	7 cut(s)	115, 181, 420, 759, 1788, 1895, 2537
HpyAV	CCTTC	7 cut(s)	100, 121, 780, 879, 1366, 1430, 1967
HpyCH4III	ACNGT	9 cut(s)	99, 852, 1221, 1262, 1671, 1800, 2104, 2284, 2509
HpyCH4IV	ACGT	4 cut(s)	67, 1020, 2140, 2254
HpyF10VI	GCNNNNNNNGC	8 cut(s)	783, 1777, 1988, 2114, 2307, 2457, 2549, 2558
HpyF3I	CTNAG	9 cut(s)	30, 35, 192, 932, 1148, 1911, 2043, 2204, 2239
HpySE526I	ACGT	4 cut(s)	67, 1020, 2140, 2254
HspAI	GCGC	1 cut(s)	959
KpnI	GGTACC	2 cut(s)	604, 2103
Ksp22I	TGATCA	1 cut(s)	1642
Kzo9I	GATC	8 cut(s)	935, 1144, 1589, 1642, 2170, 2197, 2396, 2485
LguI	GCTCTTC	1 cut(s)	1276
Lsp1109I	GCAGC	2 cut(s)	1978, 2366
LweI	GCATC	9 cut(s)	152, 177, 506, 610, 953, 981, 1132, 1390, 1594
MabI	ACCWGGT	2 cut(s)	238, 436
MaeI	CTAG	4 cut(s)	86, 177, 2427, 2630
MaeII	ACGT	4 cut(s)	67, 1020, 2140, 2254
MaeIII	GTNAC	3 cut(s)	1333, 1535, 2329
MalI	GATC	8 cut(s)	937, 1146, 1591, 1644, 2172, 2199, 2398, 2487
MbiI	CCGCTC	1 cut(s)	2543
MboI	GATC	8 cut(s)	935, 1144, 1589, 1642, 2170, 2197, 2396, 2485
MfeI	CAATTG	2 cut(s)	2057, 2337
MflI	RGATCY	2 cut(s)	1144, 1589
MhlI	GDGCHC	7 cut(s)	537, 1288, 1321, 1467, 2386, 2453, 2614
MlsI	TGGCCA	1 cut(s)	573
MluNI	TGGCCA	1 cut(s)	573
MlyI	GAGTC	2 cut(s)	1591, 1934
MmeI	TCCRAC	3 cut(s)	224, 231, 1689
Mox20I	TGGCCA	1 cut(s)	573
Mph1103I	ATGCAT	2 cut(s)	409, 825
MroXI	GAANNNNTTC	5 cut(s)	645, 763, 804, 1440, 1503
MscI	TGGCCA	1 cut(s)	573
MslI	CAYNNNNRTG	4 cut(s)	843, 989, 1650, 2523
Msp20I	TGGCCA	1 cut(s)	573
MspA1I	CMGCKG	1 cut(s)	458
MspI	CCGG	2 cut(s)	1182, 2363
MspR9I	CCNGG	9 cut(s)	240, 311, 432, 438, 832, 1865, 1996, 2235, 2364
MunI	CAATTG	2 cut(s)	2057, 2337
Mva1269I	GAATGC	2 cut(s)	679, 823
MvaI	CCWGG	8 cut(s)	240, 311, 432, 438, 832, 1865, 1996, 2235
MvnI	CGCG	3 cut(s)	959, 961, 2310
MwoI	GCNNNNNNNGC	8 cut(s)	783, 1777, 1988, 2114, 2307, 2457, 2549, 2558
NciI	CCSGG	1 cut(s)	2364
NcoI	CCATGG	3 cut(s)	511, 880, 1703
NdeI	CATATG	2 cut(s)	1987, 2275
NdeII	GATC	8 cut(s)	935, 1144, 1589, 1642, 2170, 2197, 2396, 2485
NlaIV	GGNNCC	8 cut(s)	237, 602, 836, 1318, 1785, 2101, 2452, 2551
NmuCI	GTSAC	2 cut(s)	1333, 2329
NsiI	ATGCAT	2 cut(s)	409, 825
NspI	RCATGY	3 cut(s)	215, 453, 988
OliI	CACNNNNGTG	1 cut(s)	2523
PaeI	GCATGC	1 cut(s)	988
PaeR7I	CTCGAG	1 cut(s)	2433
PagI	TCATGA	1 cut(s)	151
PciI	ACATGT	1 cut(s)	211
PciSI	GCTCTTC	1 cut(s)	1276
PctI	GAATGC	2 cut(s)	679, 823
PdmI	GAANNNNTTC	5 cut(s)	645, 763, 804, 1440, 1503
PfeI	GAWTC	8 cut(s)	206, 585, 626, 701, 1574, 1975, 2270, 2414
PflMI	CCANNNNNTGG	3 cut(s)	512, 610, 755
PfoI	TCCNGGA	1 cut(s)	309
PkrI	GCNGC	2 cut(s)	1993, 2356
PleI	GAGTC	2 cut(s)	1591, 1934
PpsI	GAGTC	2 cut(s)	1591, 1934
PscI	ACATGT	1 cut(s)	211
PshAI	GACNNNNGTC	1 cut(s)	2217
PshBI	ATTAAT	1 cut(s)	327
Psp124BI	GAGCTC	2 cut(s)	1467, 2614
Psp6I	CCWGG	8 cut(s)	238, 309, 430, 436, 830, 1863, 1994, 2233
PspEI	GGTNACC	1 cut(s)	1535
PspFI	CCCAGC	1 cut(s)	454
PspGI	CCWGG	8 cut(s)	238, 309, 430, 436, 830, 1863, 1994, 2233
PspN4I	GGNNCC	8 cut(s)	237, 602, 836, 1318, 1785, 2101, 2452, 2551
PspPI	GGNCC	3 cut(s)	434, 660, 834
PsrI	GAACNNNNNNTAC	2 cut(s)	2017, 2049
PstNI	CAGNNNCTG	1 cut(s)	316
PsuI	RGATCY	2 cut(s)	1144, 1589
RsaI	GTAC	9 cut(s)	70, 84, 602, 792, 849, 2076, 2101, 2585, 2628
RsaNI	GTAC	9 cut(s)	69, 83, 601, 791, 848, 2075, 2100, 2584, 2627
RseI	CAYNNNNRTG	4 cut(s)	843, 989, 1650, 2523
SacI	GAGCTC	2 cut(s)	1467, 2614
SalI	GTCGAC	1 cut(s)	1005
SapI	GCTCTTC	1 cut(s)	1276
SatI	GCNGC	2 cut(s)	1992, 2355
Sau3AI	GATC	8 cut(s)	935, 1144, 1589, 1642, 2170, 2197, 2396, 2485
Sau96I	GGNCC	3 cut(s)	434, 660, 834
SchI	GAGTC	2 cut(s)	1591, 1934
ScrFI	CCNGG	9 cut(s)	240, 311, 432, 438, 832, 1865, 1996, 2235, 2364
SduI	GDGCHC	7 cut(s)	537, 1288, 1321, 1467, 2386, 2453, 2614
SexAI	ACCWGGT	2 cut(s)	238, 436
SfaNI	GCATC	9 cut(s)	152, 177, 506, 610, 953, 981, 1132, 1390, 1594
SfcI	CTRYAG	2 cut(s)	651, 1674
Sfr274I	CTCGAG	1 cut(s)	2433
SinI	GGWCC	2 cut(s)	434, 660
SlaI	CTCGAG	1 cut(s)	2433
SmiMI	CAYNNNNRTG	4 cut(s)	843, 989, 1650, 2523
SmlI	CTYRAG	4 cut(s)	138, 520, 1404, 2433
SmoI	CTYRAG	4 cut(s)	138, 520, 1404, 2433
SpeI	ACTAGT	1 cut(s)	2426
SphI	GCATGC	1 cut(s)	988
SsiI	CCGC	6 cut(s)	458, 2155, 2310, 2320, 2543, 2624
SspI	AATATT	1 cut(s)	894
SspMI	CTAG	4 cut(s)	86, 177, 2427, 2630
SstI	GAGCTC	2 cut(s)	1467, 2614
StyD4I	CCNGG	9 cut(s)	238, 309, 430, 436, 830, 1863, 1994, 2233, 2362
StyI	CCWWGG	4 cut(s)	511, 880, 1703, 2215
TaaI	ACNGT	9 cut(s)	99, 852, 1221, 1262, 1671, 1800, 2104, 2284, 2509
TaiI	ACGT	4 cut(s)	70, 1023, 2143, 2257
TaqI	TCGA	5 cut(s)	1006, 2079, 2372, 2421, 2434
TatI	WGTACW	4 cut(s)	82, 790, 847, 2074
TfiI	GAWTC	8 cut(s)	206, 585, 626, 701, 1574, 1975, 2270, 2414
TscAI	CASTG	2 cut(s)	615, 1384
TseFI	GTSAC	2 cut(s)	1333, 2329
TseI	GCWGC	2 cut(s)	1991, 2354
Tsp45I	GTSAC	2 cut(s)	1333, 2329
TspRI	CASTG	2 cut(s)	615, 1384
Van91I	CCANNNNNTGG	3 cut(s)	512, 610, 755
VneI	GTGCAC	1 cut(s)	2382
VpaK11BI	GGWCC	2 cut(s)	434, 660
VspI	ATTAAT	1 cut(s)	327
XapI	RAATTY	6 cut(s)	254, 719, 901, 964, 1166, 2633
XceI	RCATGY	3 cut(s)	215, 453, 988
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1992
XhoI	CTCGAG	1 cut(s)	2433
XmiI	GTMKAC	2 cut(s)	1006, 1677
XmnI	GAANNNNTTC	5 cut(s)	645, 763, 804, 1440, 1503
XspI	CTAG	4 cut(s)	86, 177, 2427, 2630
Zsp2I	ATGCAT	2 cut(s)	409, 825

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.