Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0009110

Physical Location & Seq

Genomic Coordinates Forward (+)

12717229 .. 12724705

Gene Structure 7477 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027559

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2733 bp

ATGAAGTTCACCTTGTGTTCATCATCTTCTGAGCTTAGATATCCTGTTGTTGAGGGATTGCCCAGACGTACAAACAAGAATATACTAGAGCCTGAACCGTCGGTGAAGGTTGATAATTTTGTTAAGAAGGTTAATGCTCAAGCATCTAATCATGATACGAGGAAGCTGGTGATGTCTAGCAATTTGATGCCTCAGCACATACAAGAATCACATGTGGGAAAGAAACAACTGGAGGCACCTGGTCGGAAGTTGGAACTTGTGAGGACATTATTGATTGACAACTATGATAGCTACACATACAATATATACCAGGAGCTGTCTGTCATTAATGGCTTACCTCCTGTTGTGGTGCGAAATGATGATTTGACATGGAAAGATGTTTGCCATTATTTGTATGAAGAAAATGCATTTGATAACATTGTTATATCACCTGGACCTGGTTCTCCTACATGCCCAGCGGACATAGGTAAGATTATCATATATTTACTAAGTACATGCCTGCAAAGGCCACTGTTAACAATGGTATATACATACAAACTGCAAAATGATGCTTTAGGTTATGTGCATGGTGCTAAAGTTGTTCACGCATCTGAACCAGTCCATGGACGCTTGAGTGAAATTGAGCACAATGGGTGCGTACTTTTCAATGACATTCCCTCTGGCCACAACTCTGGATTCAAGGTGGTTCGGTACCATTCACTGGTCATAGATGCTGAATCACTCCCAGATGAACTCATTCCTATAGCATGGACCTCTTCTGTGAATGCACTTTCTTTCATTGAGACCAAAGAATCTGATGTTGCCTCTAAATTTGCTGGTGGGTCTTTCTCAAGGAAACCAAAAAATGGAAGTTATTCACCTTCCAGCCATTCTGGCAAAGTACGGAGTGAAAGGGTTCTCATGGGCATTATGCATTCTTCCAGGCCCCATTATGGTGTACAGTTCCATCCAGAGAGTGTTGCTACCTGCCATGGAAGGCAAATATTCAAGAATTTTAGAAAAATCACAGAGGATTATTGGCTGAGATCAAGGGCATCATTCATCAACATGCGAAATTTTCCTTTTAATGCACGCATGCAGATGCCCCATGTTGGTCAACTATGGACAGACGTTCCAAAACACAGGCAACCACTAAATAATGCAGATGGTCAATTATATAAAAATGCAAATAACAGAAGTTTCTCTGACATGGTTGATATGGTAAACCTTTTGCATCCAAGCATTACTATTAAAGATCTGAGGTTGAAATGGAAGAAATTTAAGCACTTGGCCGGTCAAGTTGGTGGAGCAAGAAATATATTTTGTGAACTGTTTGGACAAGATAAAGCTGAAAACACCTTTTGGTTAGACAGTTCATCAATAGAGAAGAGAAGAGCACGCTTTTCATATATGGGAGGAAAAGGTGGAGCCCTTTGGAAGCAAGTGACCTTTAAATTGTCTGATAAAAGTGATATGGCTTTGAAGGGCAGTGGTCTTCTATCTGTTCAGGATGCTCAAGGCTCTATCAAATGTTCGTTTTTGGAAGAAGGTTTTCTTGATTTTCTGAAAAAGGAGCTCTTGTCATTTTGTTATGATGAAAAGGATTATGAAGAACTTCCATTTGATTTTCATGGTGGATATATTGGTTACCTCGGGTATAACCTAAAAGTTGAATGTGGTGTGGATTCTAATCGCCACAGATCCGAGACTCCAGATGCTTGCTTTTTCTTTGCTGATAACCTTGTAGTTGTTGATCATCGTAATGATGATGTTTACATACTGTCTGTAGACGAAGTCTGCAGACCTTTGACACCATGGTTGGATGATACAGAACAGAAGCTTCTCAACTTAAAAGCTTCTGCTACAGGGGAGGGAAAGAAGCGAACTTTGCTGGCTCTAAAATCTTCACTGTGTCAAGGAAGTTTCCTTGCTGATAAATCAAGAGAAGCGTATATAGAAGATGTTGACAAGTGCCTGGAATACATTAAAGATGGTGAAAGTTATGAATTATGTCTGACAACTCAGATGAGAAAAAGAATTGGGGACATAGACTCATTGGGACTTTACCTTCATTTAAGAGAGAAGAATCCAGCACCATATGCTGCCTGGCTTAATTTTTCAGATGAAGACCTATGTATCTGCTGTTCTTCCCCTGAGCGTTTCTTACAATTGGATAGAAATGGTGTACTCGAAGCAAAACCCATTAAGGGTACCGTAGCTCGTGGTGTGACACCAGAGGAAGATGAACAACGTAAACTACAACTGCAGTATAGTGAAAAGGATCAAGCTGAAAATCTGATGATAGTTGATCTTCTAAGGAATGATCTTGGCCGTGTATGTGAGCCTGGCTCAGTTCATGTGCCACGTCTTATGGATGTGGAATCATATGCTACGGTTCATACTATGGTCAGCACAATTCGTGGGCAAAAGCGGTCTGATGTGACTGCAATTGATTGTGTGAGAGCAGCATTTCCGGGTGGTTCGATGACTGGTGCACCGAAATTGAGATCAATGGAACTTCTTGATTCAATCGAAACTAGTTCTCGAGGTATCTACTCGGGCTCCATTGGCTTTTTCTCATATAACCAGACATTTGATCTAAATATAGTTATTAGAACTGTTGTTATACACGAGGGTGAAGCTTCAATAGGAGCGGGGGGAGCCATTGTTGCCCTATCAAATCCTGAAGACGAATACGAGGAAATGGTTTTGAAAACACGAGCTCCAGCAAAAGCTGTACTAGAATTTTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006766 GO:0006767 GO:0006807 GO:0008150 GO:0008152 GO:0008153 GO:0008483 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009108 GO:0009110 GO:0009396 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016769 GO:0017144 GO:0018130 GO:0019438 GO:0019752 GO:0032787 GO:0034641 GO:0042364 GO:0042398 GO:0042537 GO:0042558 GO:0042559 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046417 GO:0046482 GO:0046483 GO:0046653 GO:0046654 GO:0046655 GO:0046656 GO:0046820 GO:0051186 GO:0051188 GO:0071704 GO:0072330 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00790 map00790

Pfam Domains

Protein Families

Anth_synt_I_N Chorismate_bind GATase

Protein Analysis

911

Amino Acids

102.31

Weight (kDa)

6.39

Isoelectric Point (pI)

44.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase	PF00117	90 - 256	1.3e-16	Glutamine amidotransferase class-I
GATase	PF00117	297 - 332	5.7e-08	Glutamine amidotransferase class-I
Anth_synt_I_N	PF04715	442 - 586	2.4e-15	Anthranilate synthase component I, N terminal region
Chorismate_bind	PF00425	641 - 898	1.4e-85	chorismate binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28880
fragaria_vesca	FvH4_7g19690
malus_domestica	MD01G1109300.v1.1 MD07G1175300.v1.1
prunus_persica	Prupe.2G214500_v2.0.a1
pyrus_communis	pycom01g13660 pycom07g16970
rosa_chinensis	RchiOBHm_Chr1g0363521
rosa_laevigata	RLG00000027559
rosa_multiflora	Rmu_ssc0000433.1_g000001
rosa_roxburghii	Rroxscaffold_4G00293090
rosa_rugosa	Rorug01G0308100
rosa_samantha	Rh1AG316800 Rh1BG280200 Rh1CG296300 Rh1DG311400 Rh5DG228600
rosa_wichuraiana	Rw0G000800 Rw1G028080 Rw2G022180 Rw3G029370 Rw5G038670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	974
Acc65I	GGTACC	2 cut(s)	690, 2189
AccB1I	GGYRCC	3 cut(s)	235, 690, 2189
AccB7I	CCANNNNNTGG	3 cut(s)	602, 700, 845
AccBSI	CCGCTC	1 cut(s)	2633
AccI	GTMKAC	1 cut(s)	1767
AciI	CCGC	3 cut(s)	458, 2410, 2633
AclWI	GGATC	2 cut(s)	1674, 2268
AcoI	YGGCCR	3 cut(s)	661, 1269, 2308
AcsI	RAATTY	5 cut(s)	809, 991, 1054, 1256, 2723
AcuI	CTGAAG	1 cut(s)	2685
AdeI	CACNNNGTG	1 cut(s)	15
AfaI	GTAC	9 cut(s)	70, 493, 639, 692, 882, 939, 2166, 2191, 2718
AfiI	CCNNNNNNNGG	7 cut(s)	437, 602, 700, 845, 932, 1091, 2186
AflIII	ACRYGT	1 cut(s)	211
AgsI	TTSAA	9 cut(s)	646, 679, 988, 1246, 1462, 1652, 2508, 2625, 2692
AhlI	ACTAGT	1 cut(s)	2516
AjiI	CACGTC	1 cut(s)	2345
AjnI	CCWGG	8 cut(s)	238, 309, 430, 436, 920, 1953, 2084, 2323
AleI	CACNNNNGTG	1 cut(s)	2613
Alw21I	GWGCWC	5 cut(s)	627, 1378, 1557, 2476, 2704
Alw26I	GTCTC	2 cut(s)	776, 1679
Alw44I	GTGCAC	1 cut(s)	2472
AlwI	GGATC	2 cut(s)	1674, 2268
AlwNI	CAGNNNCTG	1 cut(s)	316
Ama87I	CYCGRG	3 cut(s)	1631, 2523, 2536
AoxI	GGCC	5 cut(s)	506, 661, 923, 1269, 2308
ApaLI	GTGCAC	1 cut(s)	2472
ApeKI	GCWGC	2 cut(s)	2081, 2444
ApoI	RAATTY	5 cut(s)	809, 991, 1054, 1256, 2723
AseI	ATTAAT	1 cut(s)	327
Asp700I	GAANNNNTTC	5 cut(s)	735, 853, 894, 1530, 1593
Asp718I	GGTACC	2 cut(s)	690, 2189
AspS9I	GGNCC	3 cut(s)	434, 750, 924
AsuC2I	CCSGG	1 cut(s)	2454
AsuHPI	GGTGA	6 cut(s)	115, 181, 420, 849, 1985, 2627
AvaI	CYCGRG	3 cut(s)	1631, 2523, 2536
AvaII	GGWCC	2 cut(s)	434, 750
BaeGI	GKGCMC	1 cut(s)	2476
BaeI	ACNNNNGTAYC	2 cut(s)	2098, 2131
BalI	TGGCCA	1 cut(s)	663
BanI	GGYRCC	3 cut(s)	235, 690, 2189
BanII	GRGCYC	4 cut(s)	1411, 1557, 2543, 2704
BauI	CACGAG	3 cut(s)	2199, 2609, 2697
BbsI	GAAGAC	3 cut(s)	1466, 2112, 2673
Bbv12I	GWGCWC	5 cut(s)	627, 1378, 1557, 2476, 2704
BbvCI	CCTCAGC	1 cut(s)	192
BbvI	GCAGC	2 cut(s)	2068, 2456
BccI	CCATC	3 cut(s)	954, 1139, 1964
BceAI	ACGGC	1 cut(s)	2295
BciT130I	CCWGG	8 cut(s)	240, 311, 432, 438, 922, 1955, 2086, 2325
BclI	TGATCA	1 cut(s)	1732
BcnI	CCSGG	1 cut(s)	2454
BcoDI	GTCTC	2 cut(s)	776, 1679
BcuI	ACTAGT	1 cut(s)	2516
BfaI	CTAG	4 cut(s)	86, 177, 2517, 2720
BfmI	CTRYAG	5 cut(s)	741, 1764, 1777, 1842, 2243
BfuAI	ACCTGC	1 cut(s)	974
BglI	GCCNNNNNGGC	1 cut(s)	873
BglII	AGATCT	1 cut(s)	1234
BisI	GCNGC	2 cut(s)	2082, 2445
BlsI	GCNGC	2 cut(s)	2083, 2446
Bme1390I	CCNGG	9 cut(s)	240, 311, 432, 438, 922, 1955, 2086, 2325, 2454
Bme18I	GGWCC	2 cut(s)	434, 750
BmeT110I	CYCGRG	3 cut(s)	1631, 2523, 2536
BmgBI	CACGTC	1 cut(s)	2345
BmgT120I	GGNCC	3 cut(s)	434, 750, 924
BmiI	GGNNCC	7 cut(s)	237, 692, 926, 1408, 2191, 2542, 2641
BmrFI	CCNGG	9 cut(s)	240, 311, 432, 438, 922, 1955, 2086, 2325, 2454
BpiI	GAAGAC	3 cut(s)	1466, 2112, 2673
BplI	GAGNNNNNCTC	2 cut(s)	2312, 2344
BpmI	CTGGAG	3 cut(s)	251, 1674, 2688
Bpu10I	CCTNAGC	2 cut(s)	192, 2133
BpuEI	CTTGAG	4 cut(s)	123, 631, 814, 1479
BpuMI	CCSGG	1 cut(s)	2454
BsaBI	GATNNNNATC	1 cut(s)	1668
BsaI	GGTCTC	1 cut(s)	776
BsaJI	CCNNGG	4 cut(s)	601, 970, 1630, 1793
BsaXI	ACNNNNNCTCC	8 cut(s)	1544, 1574, 2525, 2555, 2631, 2661, 2686, 2716
Bsc4I	CCNNNNNNNGG	7 cut(s)	437, 602, 700, 845, 932, 1091, 2186
Bse118I	RCCGGY	1 cut(s)	1271
Bse1I	ACTGG	4 cut(s)	234, 596, 705, 2473
Bse8I	GATNNNNATC	1 cut(s)	1668
BseBI	CCWGG	8 cut(s)	240, 311, 432, 438, 922, 1955, 2086, 2325
BseDI	CCNNGG	4 cut(s)	601, 970, 1630, 1793
BseGI	GGATG	5 cut(s)	946, 1213, 1495, 1807, 2359
BseJI	GATNNNNATC	1 cut(s)	1668
BseLI	CCNNNNNNNGG	7 cut(s)	437, 602, 700, 845, 932, 1091, 2186
BseMII	CTCAG	7 cut(s)	21, 206, 1013, 1229, 2015, 2124, 2343
BseNI	ACTGG	4 cut(s)	234, 596, 705, 2473
BseSI	GKGCMC	1 cut(s)	2476
BseXI	GCAGC	2 cut(s)	2068, 2456
BseYI	CCCAGC	1 cut(s)	454
BshFI	GGCC	5 cut(s)	508, 663, 925, 1271, 2310
BshNI	GGYRCC	3 cut(s)	235, 690, 2189
BsiHKAI	GWGCWC	5 cut(s)	627, 1378, 1557, 2476, 2704
BsiHKCI	CYCGRG	3 cut(s)	1631, 2523, 2536
BsiSI	CCGG	2 cut(s)	1272, 2453
BslFI	GGGAC	2 cut(s)	2036, 2052
BslI	CCNNNNNNNGG	7 cut(s)	437, 602, 700, 845, 932, 1091, 2186
BsmAI	GTCTC	2 cut(s)	776, 1679
BsmFI	GGGAC	2 cut(s)	2036, 2052
BsmI	GAATGC	2 cut(s)	769, 913
BsnI	GGCC	5 cut(s)	508, 663, 925, 1271, 2310
Bso31I	GGTCTC	1 cut(s)	776
BsoBI	CYCGRG	3 cut(s)	1631, 2523, 2536
Bsp1286I	GDGCHC	7 cut(s)	627, 1378, 1411, 1557, 2476, 2543, 2704
Bsp1407I	TGTACA	1 cut(s)	937
Bsp143I	GATC	9 cut(s)	1025, 1234, 1679, 1732, 2260, 2287, 2302, 2486, 2575
Bsp19I	CCATGG	3 cut(s)	601, 970, 1793
BspACI	CCGC	3 cut(s)	458, 2410, 2633
BspANI	GGCC	5 cut(s)	508, 663, 925, 1271, 2310
BspCNI	CTCAG	7 cut(s)	22, 205, 1014, 1230, 2014, 2125, 2342
BspHI	TCATGA	1 cut(s)	151
BspLI	GGNNCC	7 cut(s)	237, 692, 926, 1408, 2191, 2542, 2641
BspMAI	CTGCAG	2 cut(s)	1781, 2247
BspMI	ACCTGC	1 cut(s)	974
BspPI	GGATC	2 cut(s)	1674, 2268
BspQI	GCTCTTC	1 cut(s)	1366
BspT107I	GGYRCC	3 cut(s)	235, 690, 2189
BspTNI	GGTCTC	1 cut(s)	776
BsrBI	CCGCTC	1 cut(s)	2633
BsrFI	RCCGGY	1 cut(s)	1271
BsrGI	TGTACA	1 cut(s)	937
BsrI	ACTGG	4 cut(s)	234, 596, 705, 2473
BssAI	RCCGGY	1 cut(s)	1271
BssECI	CCNNGG	4 cut(s)	601, 970, 1630, 1793
BssMI	GATC	9 cut(s)	1025, 1234, 1679, 1732, 2260, 2287, 2302, 2486, 2575
BssSI	CACGAG	3 cut(s)	2199, 2609, 2697
BssT1I	CCWWGG	3 cut(s)	601, 970, 1793
Bst2BI	CACGAG	3 cut(s)	2199, 2609, 2697
Bst2UI	CCWGG	8 cut(s)	240, 311, 432, 438, 922, 1955, 2086, 2325
Bst6I	CTCTTC	3 cut(s)	760, 1361, 1366
BstAPI	GCANNNNNTGC	1 cut(s)	2078
BstAUI	TGTACA	1 cut(s)	937
BstC8I	GCNNGC	6 cut(s)	500, 1072, 1076, 1378, 1699, 1872
BstDSI	CCRYGG	3 cut(s)	601, 970, 1793
BstEII	GGTNACC	1 cut(s)	1625
BstF5I	GGATG	5 cut(s)	946, 1213, 1495, 1807, 2359
BstKTI	GATC	9 cut(s)	1028, 1237, 1682, 1735, 2263, 2290, 2305, 2489, 2578
BstMAI	GTCTC	2 cut(s)	776, 1679
BstMBI	GATC	9 cut(s)	1025, 1234, 1679, 1732, 2260, 2287, 2302, 2486, 2575
BstMWI	GCNNNNNNNGC	6 cut(s)	873, 1867, 2078, 2547, 2639, 2648
BstNI	CCWGG	8 cut(s)	240, 311, 432, 438, 922, 1955, 2086, 2325
BstNSI	RCATGY	5 cut(s)	215, 453, 498, 1051, 1078
BstPI	GGTNACC	1 cut(s)	1625
BstSCI	CCNGG	9 cut(s)	238, 309, 430, 436, 920, 1953, 2084, 2323, 2452
BstSFI	CTRYAG	5 cut(s)	741, 1764, 1777, 1842, 2243
BstSLI	GKGCMC	1 cut(s)	2476
BstV1I	GCAGC	2 cut(s)	2068, 2456
BstV2I	GAAGAC	3 cut(s)	1466, 2112, 2673
BstX2I	RGATCY	2 cut(s)	1234, 1679
BstXI	CCANNNNNNTGG	1 cut(s)	671
BstYI	RGATCY	2 cut(s)	1234, 1679
BsuRI	GGCC	5 cut(s)	508, 663, 925, 1271, 2310
BtgI	CCRYGG	3 cut(s)	601, 970, 1793
BtrI	CACGTC	1 cut(s)	2345
BtsCI	GGATG	5 cut(s)	946, 1213, 1495, 1807, 2359
BtsI	GCAGTG	1 cut(s)	1474
BtsIMutI	CAGTG	4 cut(s)	509, 698, 1474, 1886
BveI	ACCTGC	1 cut(s)	974
Cac8I	GCNNGC	6 cut(s)	500, 1072, 1076, 1378, 1699, 1872
CaiI	CAGNNNCTG	1 cut(s)	316
CciI	TCATGA	1 cut(s)	151
Cfr10I	RCCGGY	1 cut(s)	1271
Cfr13I	GGNCC	3 cut(s)	434, 750, 924
CseI	GACGC	1 cut(s)	615
CsiI	ACCWGGT	2 cut(s)	238, 436
Csp6I	GTAC	9 cut(s)	69, 492, 638, 691, 881, 938, 2165, 2190, 2717
CviQI	GTAC	9 cut(s)	69, 492, 638, 691, 881, 938, 2165, 2190, 2717
DpnI	GATC	9 cut(s)	1027, 1236, 1681, 1734, 2262, 2289, 2304, 2488, 2577
DpnII	GATC	9 cut(s)	1025, 1234, 1679, 1732, 2260, 2287, 2302, 2486, 2575
DraI	TTTAAA	1 cut(s)	1432
DraIII	CACNNNGTG	1 cut(s)	15
EaeI	YGGCCR	3 cut(s)	661, 1269, 2308
Eam1104I	CTCTTC	3 cut(s)	760, 1361, 1366
EarI	CTCTTC	3 cut(s)	760, 1361, 1366
Ecl136II	GAGCTC	2 cut(s)	1555, 2702
Eco130I	CCWWGG	3 cut(s)	601, 970, 1793
Eco24I	GRGCYC	4 cut(s)	1411, 1557, 2543, 2704
Eco31I	GGTCTC	1 cut(s)	776
Eco32I	GATATC	1 cut(s)	41
Eco47I	GGWCC	2 cut(s)	434, 750
Eco53kI	GAGCTC	2 cut(s)	1555, 2702
Eco57I	CTGAAG	1 cut(s)	2685
Eco88I	CYCGRG	3 cut(s)	1631, 2523, 2536
Eco91I	GGTNACC	1 cut(s)	1625
EcoICRI	GAGCTC	2 cut(s)	1555, 2702
EcoO109I	RGGNCCY	1 cut(s)	924
EcoO65I	GGTNACC	1 cut(s)	1625
EcoRII	CCWGG	8 cut(s)	238, 309, 430, 436, 920, 1953, 2084, 2323
EcoRV	GATATC	1 cut(s)	41
EcoT14I	CCWWGG	3 cut(s)	601, 970, 1793
EcoT22I	ATGCAT	2 cut(s)	409, 915
EcoT38I	GRGCYC	4 cut(s)	1411, 1557, 2543, 2704
ErhI	CCWWGG	3 cut(s)	601, 970, 1793
FalI	AAGNNNNNCTT	5 cut(s)	28, 1518, 1550, 1541, 1573
FaqI	GGGAC	2 cut(s)	2036, 2052
FauI	CCCGC	1 cut(s)	2626
FauNDI	CATATG	2 cut(s)	2077, 2365
FbaI	TGATCA	1 cut(s)	1732
FblI	GTMKAC	1 cut(s)	1767
Fnu4HI	GCNGC	2 cut(s)	2082, 2445
FokI	GGATG	5 cut(s)	933, 1200, 1502, 1814, 2366
FriOI	GRGCYC	4 cut(s)	1411, 1557, 2543, 2704
Fsp4HI	GCNGC	2 cut(s)	2082, 2445
FspBI	CTAG	4 cut(s)	86, 177, 2517, 2720
GluI	GCNGC	2 cut(s)	2082, 2445
GsaI	CCCAGC	1 cut(s)	458
GsuI	CTGGAG	3 cut(s)	251, 1674, 2688
HaeIII	GGCC	5 cut(s)	508, 663, 925, 1271, 2310
HapII	CCGG	2 cut(s)	1272, 2453
HgaI	GACGC	1 cut(s)	615
HincII	GTYRAC	3 cut(s)	516, 1097, 1945
HindII	GTYRAC	3 cut(s)	516, 1097, 1945
HindIII	AAGCTT	3 cut(s)	1817, 1833, 2619
HpaI	GTTAAC	1 cut(s)	516
HpaII	CCGG	2 cut(s)	1272, 2453
HphI	GGTGA	6 cut(s)	115, 181, 420, 849, 1985, 2627
Hpy99I	CGWCG	1 cut(s)	103
HpyAV	CCTTC	7 cut(s)	100, 121, 870, 969, 1456, 1520, 2057
HpyCH4IV	ACGT	4 cut(s)	67, 1110, 2230, 2344
HpyF10VI	GCNNNNNNNGC	6 cut(s)	873, 1867, 2078, 2547, 2639, 2648
HpySE526I	ACGT	4 cut(s)	67, 1110, 2230, 2344
KpnI	GGTACC	2 cut(s)	694, 2193
Ksp22I	TGATCA	1 cut(s)	1732
KspAI	GTTAAC	1 cut(s)	516
Kzo9I	GATC	9 cut(s)	1025, 1234, 1679, 1732, 2260, 2287, 2302, 2486, 2575
LguI	GCTCTTC	1 cut(s)	1366
LmnI	GCTCC	8 cut(s)	313, 1286, 1406, 1552, 2546, 2630, 2639, 2707
Lsp1109I	GCAGC	2 cut(s)	2068, 2456
MabI	ACCWGGT	2 cut(s)	238, 436
MaeI	CTAG	4 cut(s)	86, 177, 2517, 2720
MaeII	ACGT	4 cut(s)	67, 1110, 2230, 2344
MaeIII	GTNAC	4 cut(s)	1423, 1625, 2206, 2419
MalI	GATC	9 cut(s)	1027, 1236, 1681, 1734, 2262, 2289, 2304, 2488, 2577
MbiI	CCGCTC	1 cut(s)	2633
MboI	GATC	9 cut(s)	1025, 1234, 1679, 1732, 2260, 2287, 2302, 2486, 2575
MfeI	CAATTG	2 cut(s)	2147, 2427
MflI	RGATCY	2 cut(s)	1234, 1679
MhlI	GDGCHC	7 cut(s)	627, 1378, 1411, 1557, 2476, 2543, 2704
MlsI	TGGCCA	1 cut(s)	663
MluNI	TGGCCA	1 cut(s)	663
MlyI	GAGTC	2 cut(s)	1681, 2024
MmeI	TCCRAC	3 cut(s)	224, 231, 1779
Mox20I	TGGCCA	1 cut(s)	663
Mph1103I	ATGCAT	2 cut(s)	409, 915
MroXI	GAANNNNTTC	5 cut(s)	735, 853, 894, 1530, 1593
MscI	TGGCCA	1 cut(s)	663
MslI	CAYNNNNRTG	5 cut(s)	933, 1046, 1079, 1740, 2613
Msp20I	TGGCCA	1 cut(s)	663
MspA1I	CMGCKG	1 cut(s)	458
MspI	CCGG	2 cut(s)	1272, 2453
MspR9I	CCNGG	9 cut(s)	240, 311, 432, 438, 922, 1955, 2086, 2325, 2454
MunI	CAATTG	2 cut(s)	2147, 2427
Mva1269I	GAATGC	2 cut(s)	769, 913
MvaI	CCWGG	8 cut(s)	240, 311, 432, 438, 922, 1955, 2086, 2325
MwoI	GCNNNNNNNGC	6 cut(s)	873, 1867, 2078, 2547, 2639, 2648
NciI	CCSGG	1 cut(s)	2454
NcoI	CCATGG	3 cut(s)	601, 970, 1793
NdeI	CATATG	2 cut(s)	2077, 2365
NdeII	GATC	9 cut(s)	1025, 1234, 1679, 1732, 2260, 2287, 2302, 2486, 2575
NlaIV	GGNNCC	7 cut(s)	237, 692, 926, 1408, 2191, 2542, 2641
NmuCI	GTSAC	3 cut(s)	1423, 2206, 2419
NsiI	ATGCAT	2 cut(s)	409, 915
NspI	RCATGY	5 cut(s)	215, 453, 498, 1051, 1078
OliI	CACNNNNGTG	1 cut(s)	2613
PaeI	GCATGC	1 cut(s)	1078
PaeR7I	CTCGAG	1 cut(s)	2523
PagI	TCATGA	1 cut(s)	151
PciI	ACATGT	1 cut(s)	211
PciSI	GCTCTTC	1 cut(s)	1366
PctI	GAATGC	2 cut(s)	769, 913
PdmI	GAANNNNTTC	5 cut(s)	735, 853, 894, 1530, 1593
PfeI	GAWTC	8 cut(s)	206, 675, 716, 791, 1664, 2065, 2360, 2504
PflFI	GACNNNGTC	1 cut(s)	1772
PflMI	CCANNNNNTGG	3 cut(s)	602, 700, 845
PkrI	GCNGC	2 cut(s)	2083, 2446
PleI	GAGTC	2 cut(s)	1681, 2024
PpsI	GAGTC	2 cut(s)	1681, 2024
PscI	ACATGT	1 cut(s)	211
PshBI	ATTAAT	1 cut(s)	327
Psp124BI	GAGCTC	2 cut(s)	1557, 2704
Psp6I	CCWGG	8 cut(s)	238, 309, 430, 436, 920, 1953, 2084, 2323
PspEI	GGTNACC	1 cut(s)	1625
PspFI	CCCAGC	1 cut(s)	454
PspGI	CCWGG	8 cut(s)	238, 309, 430, 436, 920, 1953, 2084, 2323
PspN4I	GGNNCC	7 cut(s)	237, 692, 926, 1408, 2191, 2542, 2641
PspPI	GGNCC	3 cut(s)	434, 750, 924
PsrI	GAACNNNNNNTAC	2 cut(s)	2107, 2139
PstI	CTGCAG	2 cut(s)	1781, 2247
PstNI	CAGNNNCTG	1 cut(s)	316
PsuI	RGATCY	2 cut(s)	1234, 1679
PsyI	GACNNNGTC	1 cut(s)	1772
RsaI	GTAC	9 cut(s)	70, 493, 639, 692, 882, 939, 2166, 2191, 2718
RsaNI	GTAC	9 cut(s)	69, 492, 638, 691, 881, 938, 2165, 2190, 2717
RseI	CAYNNNNRTG	5 cut(s)	933, 1046, 1079, 1740, 2613
SacI	GAGCTC	2 cut(s)	1557, 2704
SapI	GCTCTTC	1 cut(s)	1366
SatI	GCNGC	2 cut(s)	2082, 2445
Sau3AI	GATC	9 cut(s)	1025, 1234, 1679, 1732, 2260, 2287, 2302, 2486, 2575
Sau96I	GGNCC	3 cut(s)	434, 750, 924
SchI	GAGTC	2 cut(s)	1681, 2024
ScrFI	CCNGG	9 cut(s)	240, 311, 432, 438, 922, 1955, 2086, 2325, 2454
SduI	GDGCHC	7 cut(s)	627, 1378, 1411, 1557, 2476, 2543, 2704
SexAI	ACCWGGT	2 cut(s)	238, 436
SfcI	CTRYAG	5 cut(s)	741, 1764, 1777, 1842, 2243
Sfr274I	CTCGAG	1 cut(s)	2523
SinI	GGWCC	2 cut(s)	434, 750
SlaI	CTCGAG	1 cut(s)	2523
SmiMI	CAYNNNNRTG	5 cut(s)	933, 1046, 1079, 1740, 2613
SmlI	CTYRAG	5 cut(s)	138, 610, 829, 1494, 2523
SmoI	CTYRAG	5 cut(s)	138, 610, 829, 1494, 2523
SpeI	ACTAGT	1 cut(s)	2516
SphI	GCATGC	1 cut(s)	1078
SsiI	CCGC	3 cut(s)	458, 2410, 2633
SspI	AATATT	1 cut(s)	984
SspMI	CTAG	4 cut(s)	86, 177, 2517, 2720
SstI	GAGCTC	2 cut(s)	1557, 2704
StyD4I	CCNGG	9 cut(s)	238, 309, 430, 436, 920, 1953, 2084, 2323, 2452
StyI	CCWWGG	3 cut(s)	601, 970, 1793
TaiI	ACGT	4 cut(s)	70, 1113, 2233, 2347
TaqI	TCGA	4 cut(s)	2169, 2462, 2511, 2524
TatI	WGTACW	4 cut(s)	491, 937, 2164, 2716
TfiI	GAWTC	8 cut(s)	206, 675, 716, 791, 1664, 2065, 2360, 2504
TscAI	CASTG	4 cut(s)	516, 705, 1474, 1893
TseFI	GTSAC	3 cut(s)	1423, 2206, 2419
TseI	GCWGC	2 cut(s)	2081, 2444
Tsp45I	GTSAC	3 cut(s)	1423, 2206, 2419
TspGWI	ACGGA	1 cut(s)	898
TspRI	CASTG	4 cut(s)	516, 705, 1474, 1893
Tth111I	GACNNNGTC	1 cut(s)	1772
Van91I	CCANNNNNTGG	3 cut(s)	602, 700, 845
VneI	GTGCAC	1 cut(s)	2472
VpaK11BI	GGWCC	2 cut(s)	434, 750
VspI	ATTAAT	1 cut(s)	327
XapI	RAATTY	5 cut(s)	809, 991, 1054, 1256, 2723
XceI	RCATGY	5 cut(s)	215, 453, 498, 1051, 1078
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2082
XhoI	CTCGAG	1 cut(s)	2523
XmiI	GTMKAC	1 cut(s)	1767
XmnI	GAANNNNTTC	5 cut(s)	735, 853, 894, 1530, 1593
XspI	CTAG	4 cut(s)	86, 177, 2517, 2720
Zsp2I	ATGCAT	2 cut(s)	409, 915

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.