Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0009110

Physical Location & Seq

Genomic Coordinates Reverse (-)

55587375 .. 55594579

Gene Structure 7205 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G028080.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2718 bp

ATGAAGTTCACCTTGTGTTCATCATCTTCTGAGCTTAGATATCCTGTTGTTGAGGGGTTGCCCAGACGTACAAACAAGAATGTACTAGAGCCTGAACCGTTGGTGAAGGTTGATAATTTTGTTAAGAAGGTTAATGCTCAAGCATCTAATCATGATACGAGGAAGCTGGTGATGTCTAGCAATTTGATGCCTCAGCACATACAAGAATCACATGTGGGAAAGAAACAACTGGAGGCACCTGGTCGGAAGTTGGAATTTGTGAGGACATTATTGATTGACAACTATGATAGCTACACATACAATATATTCCAGGAGCTGTCTGTCATTAATGGCTTACCTCCTGTTGTGGTGCGAAATGATGATTTGACATGGAAAGATGTTTGCCATTACTTGTATGAAGAAAATGCATTTGATAACATTGTTATATCACCTGGACCTGGTTCTCCTACATGCCCAGCGGACATAGGAATATGTCTTCAGGTGCTGCTTGACTGCTGGAATGTCCCTATCTTGGGTGTTTGCCTTGGACATCAGGCTTTAGGTTATGTGCATGGTGCTAAAGTTGTCCACGCATCTGAACCAGTCCATGGACGCTTGAGTGAAATAGAGCACAATGGGTGCATACTTTTCAATGACATTCCCTCTGGCCACAACTCTGGATTCAAGGTGGTTCGGTACCATTCACTGGTCATAGATGCTGAATCACTCCCAGATGAACTCATTCCTATAGCATGGACCTCTTCTGTGAATGCACTTTCTTTTATTGAGACCAAAGAATCTGATGTTGCCTCTAAATTTGCTGATGGGTCTTTTTCAAGGAAACCAAAAAATGGAAGTTATTCACCTTCCAGCCATTCTGGCAAAGTACAGAGTGAAAGGGTTCTCATGGGCATTATGCATTCTTCCAGGCCCCATTATGGTGTACAGTTCCATCCAGAGAGTGTTGCTACCTGCCATGGAAGGCAAATATTCAAGAATTTTAGAAAAATCACAGAGGATTATTGGCTGAGATCAAGGGCATCATTCATCAACGCGCGAAATTTTCCTTTTAATGCACGCATGCAGATGCCCCATGTTGGTCGACTATTGACAGACGTTCCAAAACACAGGCAACCACTAAATAATGCAGATGGTCAATTATATAAAAATGTAAATAACAAAAGTTTCTCTGGCATGGTTGATATGGTAAACCTTTTGCATCCAAGCATTACTATTAAAGATCTGAGGTTGAAATGGAAGAAATTTAAGCACTTGGCCGGTCAAGTTGGTGGAGCAAGAAATATATTTTGTGAACTGTTTGGACAAGATAAAGCTGAAAACACCTTTTGGTTAGACAGTTCATCAATAGAGAAGAGAAGAGCACGCTTTTCATTTATGGGAGGAAAAGGTGGAGCCCTTTGGAAGCAAGTGACCTTTAAATTGTCTGATAAAAGTGATATGGCTTTGAAGGGCAGTGGTCTTCTATCTGTTCAGGATGCTCAAGGCTCTATCAAATGTTCATTTTTGGAAGAAGGTTTTCTTGATTTTCTGAAAAAGGAGCTCCTGTCATTTTGTTATGATGAAAAGGATTATGAAGAACTTCCATTTGATTTTCATGGTGGATATATTGGTTACCTCGGGTATAACCTAAAAGTTGAATGTGGTGTGGATTCTAATCGCCACAGATCCGAGACTCCAGATGCTTGCTTTTTCTTTGCTGATAACCTTGTAGTTGTTGATCATCGTAATGATGATGTTTACATACTGTCTGTAGACGAAGTATGCAGACCTTTGACACCATGGTTGGATGATACAGAACAAAAGCTTCTCAACTTAAAAGCTTCTGCTACAAGGGAGGGAAAGAAGCCAACTTTGCTGGCTCCACAATCTTCACCGTGTCAAGGAAGTTTCCTTGCTGATAAATCAAGAGAAGCGTATATAGAAGATGTTGACAAGTGCCTGGAATACATTAAAGATGGTGAAAGTTATGAATTATGTCTGACAACTCAGATGAGAAAAAGAATTGGGGACATAGACTCATTGGGACTATACCTTCATTTAAGAGAGAAGAATCCAGCACCATATGCTGCCTGGCTTAATTTTTCAGATGAAGACCTATGTATCTGCTGTTCTTCCCCTGAGCGTTTCTTACAATTGGATAGAAATGGTGTACTCGAAGCAAAACCCATTAAGGGTACCGTAGCTCGTGGTGCGACACCAGAGGAAGATGAACAACGTAAACTACAACTGCGGTATAGTGAAAAGGATCAAGCTGAAAATCTGATGATAGTTGATCTTCTAAGGAATGACCTTGGTCGTGTGTGTGAGCCTGGCTCAGTTCATGTGCCACGTCTTATGGATGTGGAATCATATGCTACGGTTCATACTATGGTCAGCACAATTCGCGGGCAAAAGCGGTCTGATGTGACTGCAATTGATTGTGTGAGAGCAGCATTTCCGGGTGGTTCGATGACTGGTGCACCGAAATTGAGATCAATGGAACTTCTGGATTCAATCGAAACTAGTTCTCGAGGTATCTACTCGGGCTCCATTGGCTTTTTCTCATATAACCAGACATTTGATCTAAATATAGTTATTAGAACTGTTGTTATACACGAGGGTGAAGCTTCAATAGGAGCGGGGGGAGCCATTGTTGCCCTATCAAATCCTGAAGACGAGTACGAGGAGATGGTTTTGAAAACACGAGCTCCAGCAAAAGCGGTACTAGAATTTTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006766 GO:0006767 GO:0006807 GO:0008150 GO:0008152 GO:0008153 GO:0008483 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009108 GO:0009110 GO:0009396 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016769 GO:0017144 GO:0018130 GO:0019438 GO:0019752 GO:0032787 GO:0034641 GO:0042364 GO:0042398 GO:0042537 GO:0042558 GO:0042559 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046417 GO:0046482 GO:0046483 GO:0046653 GO:0046654 GO:0046655 GO:0046656 GO:0046820 GO:0051186 GO:0051188 GO:0071704 GO:0072330 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00790 map00790

Pfam Domains

Protein Families

Anth_synt_I_N Chorismate_bind GATase

Protein Analysis

905

Amino Acids

101.4

Weight (kDa)

6.22

Isoelectric Point (pI)

45.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase	PF00117	90 - 251	1e-29	Glutamine amidotransferase class-I
GATase	PF00117	290 - 327	4.4e-08	Glutamine amidotransferase class-I
Anth_synt_I_N	PF04715	437 - 581	8.1e-16	Anthranilate synthase component I, N terminal region
Chorismate_bind	PF00425	636 - 893	4.4e-86	chorismate binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28880
fragaria_vesca	FvH4_7g19690
malus_domestica	MD01G1109300.v1.1 MD07G1175300.v1.1
prunus_persica	Prupe.2G214500_v2.0.a1
pyrus_communis	pycom01g13660 pycom07g16970
rosa_chinensis	RchiOBHm_Chr1g0363521
rosa_laevigata	RLG00000027559
rosa_multiflora	Rmu_ssc0000433.1_g000001
rosa_roxburghii	Rroxscaffold_4G00293090
rosa_rugosa	Rorug01G0308100
rosa_samantha	Rh1AG316800 Rh1BG280200 Rh1CG296300 Rh1DG311400 Rh5DG228600
rosa_wichuraiana	Rw0G000800 Rw1G028080 Rw2G022180 Rw3G029370 Rw5G038670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	959
Acc65I	GGTACC	2 cut(s)	675, 2174
AccB1I	GGYRCC	3 cut(s)	235, 675, 2174
AccB7I	CCANNNNNTGG	3 cut(s)	587, 685, 830
AccBSI	CCGCTC	1 cut(s)	2618
AccI	GTMKAC	2 cut(s)	1081, 1752
AccII	CGCG	3 cut(s)	1034, 1036, 2385
AciI	CCGC	6 cut(s)	458, 2230, 2385, 2395, 2618, 2699
AclWI	GGATC	2 cut(s)	1659, 2253
AcoI	YGGCCR	2 cut(s)	646, 1254
AcsI	RAATTY	6 cut(s)	254, 794, 976, 1039, 1241, 2708
AcuI	CTGAAG	2 cut(s)	461, 2670
AdeI	CACNNNGTG	1 cut(s)	15
AfaI	GTAC	9 cut(s)	70, 84, 677, 867, 924, 2151, 2176, 2660, 2703
AflIII	ACRYGT	1 cut(s)	211
AhlI	ACTAGT	1 cut(s)	2501
AjiI	CACGTC	1 cut(s)	2330
AjnI	CCWGG	8 cut(s)	238, 309, 430, 436, 905, 1938, 2069, 2308
AleI	CACNNNNGTG	1 cut(s)	2598
Alw21I	GWGCWC	5 cut(s)	612, 1363, 1542, 2461, 2689
Alw26I	GTCTC	2 cut(s)	761, 1664
Alw44I	GTGCAC	1 cut(s)	2457
AlwI	GGATC	2 cut(s)	1659, 2253
AlwNI	CAGNNNCTG	2 cut(s)	316, 484
Ama87I	CYCGRG	3 cut(s)	1616, 2508, 2521
AoxI	GGCC	3 cut(s)	646, 908, 1254
ApaLI	GTGCAC	1 cut(s)	2457
ApeKI	GCWGC	3 cut(s)	484, 2066, 2429
ApoI	RAATTY	6 cut(s)	254, 794, 976, 1039, 1241, 2708
AseI	ATTAAT	1 cut(s)	327
Asp700I	GAANNNNTTC	5 cut(s)	720, 838, 879, 1515, 1578
Asp718I	GGTACC	2 cut(s)	675, 2174
AspLEI	GCGC	1 cut(s)	1036
AspS9I	GGNCC	3 cut(s)	434, 735, 909
AsuC2I	CCSGG	1 cut(s)	2439
AsuHPI	GGTGA	7 cut(s)	115, 181, 420, 834, 1863, 1970, 2612
AvaI	CYCGRG	3 cut(s)	1616, 2508, 2521
AvaII	GGWCC	2 cut(s)	434, 735
BaeGI	GKGCMC	1 cut(s)	2461
BaeI	ACNNNNGTAYC	2 cut(s)	2083, 2116
BalI	TGGCCA	1 cut(s)	648
BanI	GGYRCC	3 cut(s)	235, 675, 2174
BanII	GRGCYC	4 cut(s)	1396, 1542, 2528, 2689
BauI	CACGAG	3 cut(s)	2184, 2594, 2682
BbsI	GAAGAC	4 cut(s)	467, 1451, 2097, 2658
Bbv12I	GWGCWC	5 cut(s)	612, 1363, 1542, 2461, 2689
BbvCI	CCTCAGC	1 cut(s)	192
BbvI	GCAGC	3 cut(s)	471, 2053, 2441
BccI	CCATC	5 cut(s)	797, 939, 1124, 1949, 2662
BciT130I	CCWGG	8 cut(s)	240, 311, 432, 438, 907, 1940, 2071, 2310
BclI	TGATCA	1 cut(s)	1717
BcnI	CCSGG	1 cut(s)	2439
BcoDI	GTCTC	2 cut(s)	761, 1664
BcuI	ACTAGT	1 cut(s)	2501
BfaI	CTAG	4 cut(s)	86, 177, 2502, 2705
BfmI	CTRYAG	2 cut(s)	726, 1749
BfuAI	ACCTGC	1 cut(s)	959
BglI	GCCNNNNNGGC	1 cut(s)	858
BglII	AGATCT	1 cut(s)	1219
BisI	GCNGC	3 cut(s)	485, 2067, 2430
BlsI	GCNGC	3 cut(s)	486, 2068, 2431
Bme1390I	CCNGG	9 cut(s)	240, 311, 432, 438, 907, 1940, 2071, 2310, 2439
Bme18I	GGWCC	2 cut(s)	434, 735
BmeT110I	CYCGRG	3 cut(s)	1616, 2508, 2521
BmgBI	CACGTC	1 cut(s)	2330
BmgT120I	GGNCC	3 cut(s)	434, 735, 909
BmiI	GGNNCC	8 cut(s)	237, 677, 911, 1393, 1860, 2176, 2527, 2626
BmrFI	CCNGG	9 cut(s)	240, 311, 432, 438, 907, 1940, 2071, 2310, 2439
BmsI	GCATC	9 cut(s)	152, 177, 581, 685, 1028, 1056, 1207, 1465, 1669
BoxI	GACNNNNGTC	1 cut(s)	2292
BpiI	GAAGAC	4 cut(s)	467, 1451, 2097, 2658
BplI	GAGNNNNNCTC	2 cut(s)	2297, 2329
BpmI	CTGGAG	3 cut(s)	251, 1659, 2673
Bpu10I	CCTNAGC	2 cut(s)	192, 2118
BpuEI	CTTGAG	3 cut(s)	123, 616, 1464
BpuMI	CCSGG	1 cut(s)	2439
BsaBI	GATNNNNATC	1 cut(s)	1653
BsaI	GGTCTC	1 cut(s)	761
BsaJI	CCNNGG	6 cut(s)	523, 586, 955, 1615, 1778, 2290
BsaXI	ACNNNNNCTCC	8 cut(s)	1529, 1559, 2510, 2540, 2616, 2646, 2671, 2701
Bse118I	RCCGGY	1 cut(s)	1256
Bse1I	ACTGG	4 cut(s)	234, 581, 690, 2458
Bse8I	GATNNNNATC	1 cut(s)	1653
BseBI	CCWGG	8 cut(s)	240, 311, 432, 438, 907, 1940, 2071, 2310
BseDI	CCNNGG	6 cut(s)	523, 586, 955, 1615, 1778, 2290
BseGI	GGATG	5 cut(s)	931, 1198, 1480, 1792, 2344
BseJI	GATNNNNATC	1 cut(s)	1653
BseMII	CTCAG	7 cut(s)	21, 206, 998, 1214, 2000, 2109, 2328
BseNI	ACTGG	4 cut(s)	234, 581, 690, 2458
BseRI	GAGGAG	1 cut(s)	2678
BseSI	GKGCMC	1 cut(s)	2461
BseXI	GCAGC	3 cut(s)	471, 2053, 2441
BseYI	CCCAGC	1 cut(s)	454
Bsh1236I	CGCG	3 cut(s)	1034, 1036, 2385
BshFI	GGCC	3 cut(s)	648, 910, 1256
BshNI	GGYRCC	3 cut(s)	235, 675, 2174
BsiHKAI	GWGCWC	5 cut(s)	612, 1363, 1542, 2461, 2689
BsiHKCI	CYCGRG	3 cut(s)	1616, 2508, 2521
BsiSI	CCGG	2 cut(s)	1257, 2438
BslFI	GGGAC	3 cut(s)	488, 2021, 2037
BsmAI	GTCTC	2 cut(s)	761, 1664
BsmFI	GGGAC	3 cut(s)	488, 2021, 2037
BsmI	GAATGC	2 cut(s)	754, 898
BsnI	GGCC	3 cut(s)	648, 910, 1256
Bso31I	GGTCTC	1 cut(s)	761
BsoBI	CYCGRG	3 cut(s)	1616, 2508, 2521
Bsp1286I	GDGCHC	7 cut(s)	612, 1363, 1396, 1542, 2461, 2528, 2689
Bsp1407I	TGTACA	1 cut(s)	922
Bsp143I	GATC	8 cut(s)	1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
Bsp19I	CCATGG	3 cut(s)	586, 955, 1778
BspACI	CCGC	6 cut(s)	458, 2230, 2385, 2395, 2618, 2699
BspANI	GGCC	3 cut(s)	648, 910, 1256
BspCNI	CTCAG	7 cut(s)	22, 205, 999, 1215, 1999, 2110, 2327
BspFNI	CGCG	3 cut(s)	1034, 1036, 2385
BspHI	TCATGA	1 cut(s)	151
BspLI	GGNNCC	8 cut(s)	237, 677, 911, 1393, 1860, 2176, 2527, 2626
BspMI	ACCTGC	1 cut(s)	959
BspPI	GGATC	2 cut(s)	1659, 2253
BspQI	GCTCTTC	1 cut(s)	1351
BspT107I	GGYRCC	3 cut(s)	235, 675, 2174
BspTNI	GGTCTC	1 cut(s)	761
BsrBI	CCGCTC	1 cut(s)	2618
BsrFI	RCCGGY	1 cut(s)	1256
BsrGI	TGTACA	1 cut(s)	922
BsrI	ACTGG	4 cut(s)	234, 581, 690, 2458
BssAI	RCCGGY	1 cut(s)	1256
BssECI	CCNNGG	6 cut(s)	523, 586, 955, 1615, 1778, 2290
BssMI	GATC	8 cut(s)	1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
BssSI	CACGAG	3 cut(s)	2184, 2594, 2682
BssT1I	CCWWGG	5 cut(s)	523, 586, 955, 1778, 2290
Bst2BI	CACGAG	3 cut(s)	2184, 2594, 2682
Bst2UI	CCWGG	8 cut(s)	240, 311, 432, 438, 907, 1940, 2071, 2310
Bst4CI	ACNGT	9 cut(s)	99, 927, 1296, 1337, 1746, 1875, 2179, 2359, 2584
Bst6I	CTCTTC	3 cut(s)	745, 1346, 1351
BstAPI	GCANNNNNTGC	1 cut(s)	2063
BstAUI	TGTACA	1 cut(s)	922
BstC8I	GCNNGC	6 cut(s)	1057, 1061, 1363, 1684, 1857, 2387
BstDEI	CTNAG	9 cut(s)	30, 35, 192, 1007, 1223, 1986, 2118, 2279, 2314
BstDSI	CCRYGG	3 cut(s)	586, 955, 1778
BstEII	GGTNACC	1 cut(s)	1610
BstF5I	GGATG	5 cut(s)	931, 1198, 1480, 1792, 2344
BstFNI	CGCG	3 cut(s)	1034, 1036, 2385
BstHHI	GCGC	1 cut(s)	1036
BstKTI	GATC	8 cut(s)	1013, 1222, 1667, 1720, 2248, 2275, 2474, 2563
BstMAI	GTCTC	2 cut(s)	761, 1664
BstMBI	GATC	8 cut(s)	1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
BstMWI	GCNNNNNNNGC	8 cut(s)	858, 1852, 2063, 2189, 2382, 2532, 2624, 2633
BstNI	CCWGG	8 cut(s)	240, 311, 432, 438, 907, 1940, 2071, 2310
BstNSI	RCATGY	3 cut(s)	215, 453, 1063
BstPAI	GACNNNNGTC	1 cut(s)	2292
BstPI	GGTNACC	1 cut(s)	1610
BstSCI	CCNGG	9 cut(s)	238, 309, 430, 436, 905, 1938, 2069, 2308, 2437
BstSFI	CTRYAG	2 cut(s)	726, 1749
BstSLI	GKGCMC	1 cut(s)	2461
BstUI	CGCG	3 cut(s)	1034, 1036, 2385
BstV1I	GCAGC	3 cut(s)	471, 2053, 2441
BstV2I	GAAGAC	4 cut(s)	467, 1451, 2097, 2658
BstX2I	RGATCY	2 cut(s)	1219, 1664
BstXI	CCANNNNNNTGG	1 cut(s)	656
BstYI	RGATCY	2 cut(s)	1219, 1664
BsuRI	GGCC	3 cut(s)	648, 910, 1256
BtgI	CCRYGG	3 cut(s)	586, 955, 1778
BtrI	CACGTC	1 cut(s)	2330
BtsCI	GGATG	5 cut(s)	931, 1198, 1480, 1792, 2344
BtsI	GCAGTG	1 cut(s)	1459
BtsIMutI	CAGTG	2 cut(s)	683, 1459
BveI	ACCTGC	1 cut(s)	959
Cac8I	GCNNGC	6 cut(s)	1057, 1061, 1363, 1684, 1857, 2387
CaiI	CAGNNNCTG	2 cut(s)	316, 484
CciI	TCATGA	1 cut(s)	151
CfoI	GCGC	1 cut(s)	1036
Cfr10I	RCCGGY	1 cut(s)	1256
Cfr13I	GGNCC	3 cut(s)	434, 735, 909
CseI	GACGC	1 cut(s)	600
CsiI	ACCWGGT	2 cut(s)	238, 436
Csp6I	GTAC	9 cut(s)	69, 83, 676, 866, 923, 2150, 2175, 2659, 2702
CviQI	GTAC	9 cut(s)	69, 83, 676, 866, 923, 2150, 2175, 2659, 2702
DdeI	CTNAG	9 cut(s)	30, 35, 192, 1007, 1223, 1986, 2118, 2279, 2314
DpnI	GATC	8 cut(s)	1012, 1221, 1666, 1719, 2247, 2274, 2473, 2562
DpnII	GATC	8 cut(s)	1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
DraI	TTTAAA	1 cut(s)	1417
DraIII	CACNNNGTG	1 cut(s)	15
EaeI	YGGCCR	2 cut(s)	646, 1254
Eam1104I	CTCTTC	3 cut(s)	745, 1346, 1351
EarI	CTCTTC	3 cut(s)	745, 1346, 1351
Ecl136II	GAGCTC	2 cut(s)	1540, 2687
Eco130I	CCWWGG	5 cut(s)	523, 586, 955, 1778, 2290
Eco24I	GRGCYC	4 cut(s)	1396, 1542, 2528, 2689
Eco31I	GGTCTC	1 cut(s)	761
Eco32I	GATATC	1 cut(s)	41
Eco47I	GGWCC	2 cut(s)	434, 735
Eco53kI	GAGCTC	2 cut(s)	1540, 2687
Eco57I	CTGAAG	2 cut(s)	461, 2670
Eco88I	CYCGRG	3 cut(s)	1616, 2508, 2521
Eco91I	GGTNACC	1 cut(s)	1610
EcoICRI	GAGCTC	2 cut(s)	1540, 2687
EcoO109I	RGGNCCY	1 cut(s)	909
EcoO65I	GGTNACC	1 cut(s)	1610
EcoRII	CCWGG	8 cut(s)	238, 309, 430, 436, 905, 1938, 2069, 2308
EcoRV	GATATC	1 cut(s)	41
EcoT14I	CCWWGG	5 cut(s)	523, 586, 955, 1778, 2290
EcoT22I	ATGCAT	2 cut(s)	409, 900
EcoT38I	GRGCYC	4 cut(s)	1396, 1542, 2528, 2689
ErhI	CCWWGG	5 cut(s)	523, 586, 955, 1778, 2290
FalI	AAGNNNNNCTT	3 cut(s)	28, 1503, 1535
FaqI	GGGAC	3 cut(s)	488, 2021, 2037
FauI	CCCGC	2 cut(s)	2378, 2611
FauNDI	CATATG	2 cut(s)	2062, 2350
FbaI	TGATCA	1 cut(s)	1717
FblI	GTMKAC	2 cut(s)	1081, 1752
Fnu4HI	GCNGC	3 cut(s)	485, 2067, 2430
FokI	GGATG	5 cut(s)	918, 1185, 1487, 1799, 2351
FriOI	GRGCYC	4 cut(s)	1396, 1542, 2528, 2689
Fsp4HI	GCNGC	3 cut(s)	485, 2067, 2430
FspBI	CTAG	4 cut(s)	86, 177, 2502, 2705
GlaI	GCGC	1 cut(s)	1035
GluI	GCNGC	3 cut(s)	485, 2067, 2430
GsaI	CCCAGC	1 cut(s)	458
GsuI	CTGGAG	3 cut(s)	251, 1659, 2673
HaeIII	GGCC	3 cut(s)	648, 910, 1256
HapII	CCGG	2 cut(s)	1257, 2438
HgaI	GACGC	1 cut(s)	600
HhaI	GCGC	1 cut(s)	1036
Hin6I	GCGC	1 cut(s)	1034
HinP1I	GCGC	1 cut(s)	1034
HincII	GTYRAC	2 cut(s)	1082, 1930
HindII	GTYRAC	2 cut(s)	1082, 1930
HindIII	AAGCTT	3 cut(s)	1802, 1818, 2604
HpaII	CCGG	2 cut(s)	1257, 2438
HphI	GGTGA	7 cut(s)	115, 181, 420, 834, 1863, 1970, 2612
HpyAV	CCTTC	7 cut(s)	100, 121, 855, 954, 1441, 1505, 2042
HpyCH4III	ACNGT	9 cut(s)	99, 927, 1296, 1337, 1746, 1875, 2179, 2359, 2584
HpyCH4IV	ACGT	4 cut(s)	67, 1095, 2215, 2329
HpyF10VI	GCNNNNNNNGC	8 cut(s)	858, 1852, 2063, 2189, 2382, 2532, 2624, 2633
HpyF3I	CTNAG	9 cut(s)	30, 35, 192, 1007, 1223, 1986, 2118, 2279, 2314
HpySE526I	ACGT	4 cut(s)	67, 1095, 2215, 2329
HspAI	GCGC	1 cut(s)	1034
KpnI	GGTACC	2 cut(s)	679, 2178
Ksp22I	TGATCA	1 cut(s)	1717
Kzo9I	GATC	8 cut(s)	1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
LguI	GCTCTTC	1 cut(s)	1351
Lsp1109I	GCAGC	3 cut(s)	471, 2053, 2441
LweI	GCATC	9 cut(s)	152, 177, 581, 685, 1028, 1056, 1207, 1465, 1669
MabI	ACCWGGT	2 cut(s)	238, 436
MaeI	CTAG	4 cut(s)	86, 177, 2502, 2705
MaeII	ACGT	4 cut(s)	67, 1095, 2215, 2329
MaeIII	GTNAC	3 cut(s)	1408, 1610, 2404
MalI	GATC	8 cut(s)	1012, 1221, 1666, 1719, 2247, 2274, 2473, 2562
MbiI	CCGCTC	1 cut(s)	2618
MboI	GATC	8 cut(s)	1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
MfeI	CAATTG	2 cut(s)	2132, 2412
MflI	RGATCY	2 cut(s)	1219, 1664
MhlI	GDGCHC	7 cut(s)	612, 1363, 1396, 1542, 2461, 2528, 2689
MlsI	TGGCCA	1 cut(s)	648
MluNI	TGGCCA	1 cut(s)	648
MlyI	GAGTC	2 cut(s)	1666, 2009
MmeI	TCCRAC	3 cut(s)	224, 231, 1764
Mox20I	TGGCCA	1 cut(s)	648
Mph1103I	ATGCAT	2 cut(s)	409, 900
MroXI	GAANNNNTTC	5 cut(s)	720, 838, 879, 1515, 1578
MscI	TGGCCA	1 cut(s)	648
MslI	CAYNNNNRTG	4 cut(s)	918, 1064, 1725, 2598
Msp20I	TGGCCA	1 cut(s)	648
MspA1I	CMGCKG	1 cut(s)	458
MspI	CCGG	2 cut(s)	1257, 2438
MspR9I	CCNGG	9 cut(s)	240, 311, 432, 438, 907, 1940, 2071, 2310, 2439
MunI	CAATTG	2 cut(s)	2132, 2412
Mva1269I	GAATGC	2 cut(s)	754, 898
MvaI	CCWGG	8 cut(s)	240, 311, 432, 438, 907, 1940, 2071, 2310
MvnI	CGCG	3 cut(s)	1034, 1036, 2385
MwoI	GCNNNNNNNGC	8 cut(s)	858, 1852, 2063, 2189, 2382, 2532, 2624, 2633
NciI	CCSGG	1 cut(s)	2439
NcoI	CCATGG	3 cut(s)	586, 955, 1778
NdeI	CATATG	2 cut(s)	2062, 2350
NdeII	GATC	8 cut(s)	1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
NlaIV	GGNNCC	8 cut(s)	237, 677, 911, 1393, 1860, 2176, 2527, 2626
NmuCI	GTSAC	2 cut(s)	1408, 2404
NsiI	ATGCAT	2 cut(s)	409, 900
NspI	RCATGY	3 cut(s)	215, 453, 1063
OliI	CACNNNNGTG	1 cut(s)	2598
PaeI	GCATGC	1 cut(s)	1063
PaeR7I	CTCGAG	1 cut(s)	2508
PagI	TCATGA	1 cut(s)	151
PciI	ACATGT	1 cut(s)	211
PciSI	GCTCTTC	1 cut(s)	1351
PctI	GAATGC	2 cut(s)	754, 898
PdmI	GAANNNNTTC	5 cut(s)	720, 838, 879, 1515, 1578
PfeI	GAWTC	8 cut(s)	206, 660, 701, 776, 1649, 2050, 2345, 2489
PflMI	CCANNNNNTGG	3 cut(s)	587, 685, 830
PfoI	TCCNGGA	1 cut(s)	309
PkrI	GCNGC	3 cut(s)	486, 2068, 2431
PleI	GAGTC	2 cut(s)	1666, 2009
PpsI	GAGTC	2 cut(s)	1666, 2009
PscI	ACATGT	1 cut(s)	211
PshAI	GACNNNNGTC	1 cut(s)	2292
PshBI	ATTAAT	1 cut(s)	327
Psp124BI	GAGCTC	2 cut(s)	1542, 2689
Psp6I	CCWGG	8 cut(s)	238, 309, 430, 436, 905, 1938, 2069, 2308
PspEI	GGTNACC	1 cut(s)	1610
PspFI	CCCAGC	1 cut(s)	454
PspGI	CCWGG	8 cut(s)	238, 309, 430, 436, 905, 1938, 2069, 2308
PspN4I	GGNNCC	8 cut(s)	237, 677, 911, 1393, 1860, 2176, 2527, 2626
PspPI	GGNCC	3 cut(s)	434, 735, 909
PsrI	GAACNNNNNNTAC	2 cut(s)	2092, 2124
PstNI	CAGNNNCTG	2 cut(s)	316, 484
PsuI	RGATCY	2 cut(s)	1219, 1664
RsaI	GTAC	9 cut(s)	70, 84, 677, 867, 924, 2151, 2176, 2660, 2703
RsaNI	GTAC	9 cut(s)	69, 83, 676, 866, 923, 2150, 2175, 2659, 2702
RseI	CAYNNNNRTG	4 cut(s)	918, 1064, 1725, 2598
SacI	GAGCTC	2 cut(s)	1542, 2689
SalI	GTCGAC	1 cut(s)	1080
SapI	GCTCTTC	1 cut(s)	1351
SatI	GCNGC	3 cut(s)	485, 2067, 2430
Sau3AI	GATC	8 cut(s)	1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
Sau96I	GGNCC	3 cut(s)	434, 735, 909
SchI	GAGTC	2 cut(s)	1666, 2009
ScrFI	CCNGG	9 cut(s)	240, 311, 432, 438, 907, 1940, 2071, 2310, 2439
SduI	GDGCHC	7 cut(s)	612, 1363, 1396, 1542, 2461, 2528, 2689
SexAI	ACCWGGT	2 cut(s)	238, 436
SfaNI	GCATC	9 cut(s)	152, 177, 581, 685, 1028, 1056, 1207, 1465, 1669
SfcI	CTRYAG	2 cut(s)	726, 1749
Sfr274I	CTCGAG	1 cut(s)	2508
SinI	GGWCC	2 cut(s)	434, 735
SlaI	CTCGAG	1 cut(s)	2508
SmiMI	CAYNNNNRTG	4 cut(s)	918, 1064, 1725, 2598
SmlI	CTYRAG	4 cut(s)	138, 595, 1479, 2508
SmoI	CTYRAG	4 cut(s)	138, 595, 1479, 2508
SpeI	ACTAGT	1 cut(s)	2501
SphI	GCATGC	1 cut(s)	1063
SsiI	CCGC	6 cut(s)	458, 2230, 2385, 2395, 2618, 2699
SspI	AATATT	1 cut(s)	969
SspMI	CTAG	4 cut(s)	86, 177, 2502, 2705
SstI	GAGCTC	2 cut(s)	1542, 2689
StyD4I	CCNGG	9 cut(s)	238, 309, 430, 436, 905, 1938, 2069, 2308, 2437
StyI	CCWWGG	5 cut(s)	523, 586, 955, 1778, 2290
TaaI	ACNGT	9 cut(s)	99, 927, 1296, 1337, 1746, 1875, 2179, 2359, 2584
TaiI	ACGT	4 cut(s)	70, 1098, 2218, 2332
TaqI	TCGA	5 cut(s)	1081, 2154, 2447, 2496, 2509
TatI	WGTACW	4 cut(s)	82, 865, 922, 2149
TfiI	GAWTC	8 cut(s)	206, 660, 701, 776, 1649, 2050, 2345, 2489
TscAI	CASTG	2 cut(s)	690, 1459
TseFI	GTSAC	2 cut(s)	1408, 2404
TseI	GCWGC	3 cut(s)	484, 2066, 2429
Tsp45I	GTSAC	2 cut(s)	1408, 2404
TspRI	CASTG	2 cut(s)	690, 1459
Van91I	CCANNNNNTGG	3 cut(s)	587, 685, 830
VneI	GTGCAC	1 cut(s)	2457
VpaK11BI	GGWCC	2 cut(s)	434, 735
VspI	ATTAAT	1 cut(s)	327
XapI	RAATTY	6 cut(s)	254, 794, 976, 1039, 1241, 2708
XceI	RCATGY	3 cut(s)	215, 453, 1063
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2067
XhoI	CTCGAG	1 cut(s)	2508
XmiI	GTMKAC	2 cut(s)	1081, 1752
XmnI	GAANNNNTTC	5 cut(s)	720, 838, 879, 1515, 1578
XspI	CTAG	4 cut(s)	86, 177, 2502, 2705
Zsp2I	ATGCAT	2 cut(s)	409, 900

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.