Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0009110

Physical Location & Seq

Genomic Coordinates Reverse (-)

22207923 .. 22216304

Gene Structure 8382 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1109300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2739 bp

ATGAATTTCACTTTGTGTTCGTCATCTTCCGAGCTCAGATACCCTTTGGTTGAGGGGTTGCCATATACAAACAAGAATATGCTAGAGTCTGAACCTTTTGTGAAGGTTGATAAGTTCAATAAGAAGGATAACACTCAAGTATCTAATCATGATGCTGGAAAGCTCGTGATGTCTACCAATTTGATGCCTCGGTGCTTAAATGGATCATATGTGGGAAAGAATAACCTCAAGGAACCTGGGTGGAAGCTGGAATTTGTGAGGACATTGTTGATTGACAACTATGACAGCTACACTTACAATATATACCAGGAGCTGTCTGTCATTAATGGGGTGCCTCCTGTGGTCGTGCGGAATGATGAATTGACATGGAAAGACATCCACTATTACTTGTATGAAGAAAATGCTTTTGACAATGTTGTTATATCACCTGGACCTGGTTCGCCAACATGTCCAGCAGACATAGGCATATGTCTTCAGGTGCTGCTTGACTGCTGGGATATCCCTATTTTGGGTGTTTGTCTTGGGCATCAGGCTTTAGGTTATGTGCATGGTGCTAAAGTTGTCCACGCACCTGAACCAGTCCATGGGCGGTTGAGCGAAGTTGAGCACAATGGGTGCAGACTTTTCAATGACATCCCTTCTGGTCACGATTCAGGATTCAAGGTGGTTCGGTACCATTCACTTGTCATAGATGCTGAATCACTTCCAGATGAACTCATTCCTATAGCATGGACTTCCTCCATGGATGCACTTTCTTTCATCGAAACCCAGAAGTCTGATTTTTCCTTGGAATATGCTGTGGGATATTTTTCAGAGAAACTGAAAAATGGAAGTTATTCGCCTTTCAGTCATTCTGGAAAAATGCAGAGTGTAAAGGTTCTCATGGGCATTATGCATTCTACCAGACCTCATTATGGTGTGCAGTTCCATCCAGAGAGTATTGCAACCTGTCATGGAAGACAGATATTCAAGAATTTTAGAGAGATCACTGAGGAGTATTGGCTGAATTCAAGACCGTCATTCATCAAGAAGCGAAATTTTGATTGTACAGCATGCTTGCAGATGCCCCAGCGACTGTTCACAGAAGTTCCTGGATATCAGCAATTAGTAAATAATGCAGATGGTCAGCTATATAGCAAGGCTTCTAGAAGTAGCTTATTACAGAACAGTGAAAGTAACGCAAGTTGTTCTGGTATGGTCGATATGGTAAGCCTTTTGCATCCAAGCGCTGGTGTTAAATATCTGAAGTTGAAATGGAAGAAATTTAAGCACTTGGCTGGTAAAGTTGGTGGAGCAAAAAATATTTTCTGCGAACTGTTTGGACATCATAAAGCTGAAAACACCTTTTGGTTGGACAGTTCATCGATAGAAAAGAGAAGAGCACGCTTTTCATTTATGGGAGGAAAAGGTGGAACTCTTTGGAAGCAATTGACCTTTAAATTATCTGATAGAAGTGATAAGACATTGAAGGGAAGTGGTTTTCTATCTGTTGAGGATGCTCAAGGCTCTACCAGCAGCAGATTTTTGGACGAAGGTTTTCTTGATTTTTTGAAAAAGGAGCTCCTGTCATTTTGTTACGATGAAAAGGATTATGAAGGCCTTCCATTTGATTTTCATGGTGGATATATTGGCTACATGGGATATAGCCTTAAAGTTGAATGTGGTGCATCATCTAACTGCCACAAATCCAAGACTCCAGATGCTTGCTTTTTCTTTGCCGATAATCTTGTAGTTATTGATCACTGCTCCGATGATGTTTATATACTGTCTATTAAGGAAGAATGCACAAGTACGACGCCATGGTTGGACAATACAGAACAGAAGCTTCTCAGCTTAAAAGCTTCTGCTACAGAGGAGGGAGGTGAGCCAGCTTTGCAGGCTCTTCGATCTTCGGAATGTCAAGGAAGTTTTATAGTTGATAAATCAAGAGAAGAGTATATAAAAGATGTCGATAAGTGTCTGGAATACATTAAAGACGGTGAAAGCTATGAATTATGTCTAACAACTCAGATGAGGAAAAGAATTGGGGAAATGGATTCATTAGGACTTTACCTTCACCTTCGAGAAAAAAATCCAGCACCATATGCTGCCTGGCTTAATTTTTCAAAGGAAAACCTATGTGTCTGCTGTTCTTCCCCAGAGCGGTTTCTACGGTTGGATAGAAATGGTATATTAGAAGCAAAGCCCATTAAGGGTACTATAGCTCGTGGTGCAACACTAGAAGAAGATGAGCAACGCAAATTACAACTGCAATACAGTGAAAAGGATCAAGCTGAAAATCTGATGATCGTTGATCTTCTGAGAAATGACCTTGGGCGTGTATGTGAGCCCGGCTCTGTTCATGTGCCCCATCTGATGGACGTGGAATCATATGCTACTGTTCATACTATGGTGAGCACAATTCGCGGGAAAAAGCGGTCAGATGTAAGTGCAGTTGACTGCGTGAGAGCGGCATTTCCAGGGGGTTCAATGACAGGTGCACCAAAACTGAGATCAATGGAACTTCTTGACTCAATCGAATGTAGTCCACGAGGAATCTACTCAGGCTCCATAGGGTTTTTCTCATATAACCAGACATTCGATCTCAACATAGTTATCAGAACCGTTGTTATACATGAGGGTGAAGCTTCAATAGGCGGGGGAGGAGCCATTGTTGCGTTATCAAACCCTGAAGACGAGTATGACGAAATGATACTGAAAACAAGCGCTCCGGCAAAAGCTGTAACGGAATTTCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006766 GO:0006767 GO:0006807 GO:0008150 GO:0008152 GO:0008153 GO:0008483 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009108 GO:0009110 GO:0009396 GO:0009507 GO:0009536 GO:0009987 GO:0016053 GO:0016740 GO:0016769 GO:0017144 GO:0018130 GO:0019438 GO:0019752 GO:0032787 GO:0034641 GO:0042364 GO:0042398 GO:0042537 GO:0042558 GO:0042559 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046417 GO:0046482 GO:0046483 GO:0046653 GO:0046654 GO:0046655 GO:0046656 GO:0046820 GO:0051186 GO:0051188 GO:0071704 GO:0072330 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00790 map00790

Pfam Domains

Protein Families

Anth_synt_I_N Chorismate_bind GATase

Protein Analysis

913

Amino Acids

102.04

Weight (kDa)

5.81

Isoelectric Point (pI)

46.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase	PF00117	89 - 249	6.8e-30	Glutamine amidotransferase class-I
GATase	PF00117	286 - 326	3.1e-08	Glutamine amidotransferase class-I
Anth_synt_I_N	PF04715	444 - 588	4.4e-14	Anthranilate synthase component I, N terminal region
Chorismate_bind	PF00425	643 - 900	1.2e-83	chorismate binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28880
fragaria_vesca	FvH4_7g19690
malus_domestica	MD01G1109300.v1.1 MD07G1175300.v1.1
prunus_persica	Prupe.2G214500_v2.0.a1
pyrus_communis	pycom01g13660 pycom07g16970
rosa_chinensis	RchiOBHm_Chr1g0363521
rosa_laevigata	RLG00000027559
rosa_multiflora	Rmu_ssc0000433.1_g000001
rosa_roxburghii	Rroxscaffold_4G00293090
rosa_rugosa	Rorug01G0308100
rosa_samantha	Rh1AG316800 Rh1BG280200 Rh1CG296300 Rh1DG311400 Rh5DG228600
rosa_wichuraiana	Rw0G000800 Rw1G028080 Rw2G022180 Rw3G029370 Rw5G038670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	672
AccB1I	GGYRCC	2 cut(s)	331, 672
AccB7I	CCANNNNNTGG	3 cut(s)	584, 1229, 2356
AccBSI	CCGCTC	2 cut(s)	2143, 2450
AccI	GTMKAC	1 cut(s)	173
AccII	CGCG	1 cut(s)	2406
AciI	CCGC	7 cut(s)	349, 589, 2143, 2406, 2416, 2450, 2637
AclWI	GGATC	2 cut(s)	211, 2274
AcsI	RAATTY	7 cut(s)	4, 251, 973, 1006, 1036, 1262, 2729
AcuI	CTGAAG	3 cut(s)	458, 1265, 2691
AcyI	GRCGYC	1 cut(s)	1796
AdeI	CACNNNGTG	1 cut(s)	15
AfaI	GTAC	4 cut(s)	674, 1048, 1792, 2197
AfeI	AGCGCT	2 cut(s)	1228, 2707
AfiI	CCNNNNNNNGG	9 cut(s)	434, 508, 509, 584, 914, 1229, 2142, 2192, 2356
AflIII	ACRYGT	1 cut(s)	446
AjiI	CACGTC	1 cut(s)	2362
AjnI	CCWGG	7 cut(s)	235, 306, 427, 433, 1090, 2090, 2458
Alw21I	GWGCWC	6 cut(s)	36, 609, 1384, 1563, 2399, 2482
Alw44I	GTGCAC	1 cut(s)	2478
AlwI	GGATC	2 cut(s)	211, 2274
AlwNI	CAGNNNCTG	3 cut(s)	313, 481, 1075
Aor51HI	AGCGCT	2 cut(s)	1228, 2707
AoxI	GGCC	1 cut(s)	1597
ApaLI	GTGCAC	1 cut(s)	2478
ApeKI	GCWGC	3 cut(s)	481, 1515, 2087
ApoI	RAATTY	7 cut(s)	4, 251, 973, 1006, 1036, 1262, 2729
AseI	ATTAAT	1 cut(s)	324
Asp700I	GAANNNNTTC	5 cut(s)	702, 717, 835, 1536, 1599
Asp718I	GGTACC	1 cut(s)	672
AspLEI	GCGC	2 cut(s)	1229, 2708
AspS9I	GGNCC	1 cut(s)	431
AsuC2I	CCSGG	1 cut(s)	2331
AsuHPI	GGTGA	6 cut(s)	417, 1874, 1991, 2048, 2404, 2633
AvaII	GGWCC	1 cut(s)	431
BaeGI	GKGCMC	2 cut(s)	2349, 2482
BaeI	ACNNNNGTAYC	2 cut(s)	123, 156
BanI	GGYRCC	2 cut(s)	331, 672
BanII	GRGCYC	3 cut(s)	36, 1563, 2331
BauI	CACGAG	3 cut(s)	164, 2205, 2529
BbsI	GAAGAC	3 cut(s)	464, 964, 2679
Bbv12I	GWGCWC	6 cut(s)	36, 609, 1384, 1563, 2399, 2482
BbvI	GCAGC	3 cut(s)	468, 1527, 2074
BccI	CCATC	4 cut(s)	936, 1115, 2350, 2358
BcgI	CGANNNNNNTGC	2 cut(s)	1865, 1899
BciT130I	CCWGG	7 cut(s)	237, 308, 429, 435, 1092, 2092, 2460
BclI	TGATCA	1 cut(s)	1738
BcnI	CCSGG	1 cut(s)	2331
BfaI	CTAG	3 cut(s)	83, 1146, 2219
BfmI	CTRYAG	4 cut(s)	723, 1848, 2199, 2735
BfoI	RGCGCY	2 cut(s)	1230, 2709
BisI	GCNGC	4 cut(s)	482, 1516, 2088, 2451
BlsI	GCNGC	4 cut(s)	483, 1517, 2089, 2452
Bme1390I	CCNGG	8 cut(s)	237, 308, 429, 435, 1092, 2092, 2331, 2460
Bme18I	GGWCC	1 cut(s)	431
BmgBI	CACGTC	1 cut(s)	2362
BmgT120I	GGNCC	1 cut(s)	431
BmiI	GGNNCC	5 cut(s)	234, 333, 674, 2548, 2647
BmrFI	CCNGG	8 cut(s)	237, 308, 429, 435, 1092, 2092, 2331, 2460
BpiI	GAAGAC	3 cut(s)	464, 964, 2679
BplI	GAGNNNNNCTC	2 cut(s)	2318, 2350
BpmI	CTGGAG	1 cut(s)	1680
BpuEI	CTTGAG	3 cut(s)	120, 212, 1485
BpuMI	CCSGG	1 cut(s)	2331
Bsa29I	ATCGAT	1 cut(s)	1364
BsaHI	GRCGYC	1 cut(s)	1796
BsaJI	CCNNGG	8 cut(s)	188, 236, 583, 741, 786, 1799, 2311, 2459
BsaXI	ACNNNNNCTCC	8 cut(s)	1550, 1580, 2531, 2561, 2637, 2667, 2692, 2722
Bsc4I	CCNNNNNNNGG	9 cut(s)	434, 508, 509, 584, 914, 1229, 2142, 2192, 2356
Bse1I	ACTGG	1 cut(s)	578
BseBI	CCWGG	7 cut(s)	237, 308, 429, 435, 1092, 2092, 2460
BseCI	ATCGAT	1 cut(s)	1364
BseDI	CCNNGG	8 cut(s)	188, 236, 583, 741, 786, 1799, 2311, 2459
BseGI	GGATG	6 cut(s)	375, 633, 751, 928, 1219, 1501
BseLI	CCNNNNNNNGG	9 cut(s)	434, 508, 509, 584, 914, 1229, 2142, 2192, 2356
BseMII	CTCAG	7 cut(s)	49, 981, 1843, 2021, 2291, 2480, 2556
BseNI	ACTGG	1 cut(s)	578
BseRI	GAGGAG	3 cut(s)	1007, 1868, 2658
BseSI	GKGCMC	2 cut(s)	2349, 2482
BseXI	GCAGC	3 cut(s)	468, 1527, 2074
BseYI	CCCAGC	2 cut(s)	492, 1068
BsgI	GTGCAG	3 cut(s)	637, 941, 2451
Bsh1236I	CGCG	1 cut(s)	2406
BshFI	GGCC	1 cut(s)	1599
BshNI	GGYRCC	2 cut(s)	331, 672
BshVI	ATCGAT	1 cut(s)	1364
BsiHKAI	GWGCWC	6 cut(s)	36, 609, 1384, 1563, 2399, 2482
BsiSI	CCGG	2 cut(s)	2331, 2711
BslI	CCNNNNNNNGG	9 cut(s)	434, 508, 509, 584, 914, 1229, 2142, 2192, 2356
BsmI	GAATGC	2 cut(s)	895, 1787
BsnI	GGCC	1 cut(s)	1599
Bsp1286I	GDGCHC	8 cut(s)	36, 609, 1384, 1563, 2331, 2349, 2399, 2482
Bsp1407I	TGTACA	1 cut(s)	1046
Bsp143I	GATC	9 cut(s)	203, 984, 1738, 1886, 2266, 2286, 2293, 2492, 2581
Bsp19I	CCATGG	3 cut(s)	583, 741, 1799
BspACI	CCGC	7 cut(s)	349, 589, 2143, 2406, 2416, 2450, 2637
BspANI	GGCC	1 cut(s)	1599
BspCNI	CTCAG	7 cut(s)	48, 982, 1842, 2020, 2292, 2481, 2555
BspDI	ATCGAT	1 cut(s)	1364
BspFNI	CGCG	1 cut(s)	2406
BspHI	TCATGA	1 cut(s)	148
BspLI	GGNNCC	5 cut(s)	234, 333, 674, 2548, 2647
BspPI	GGATC	2 cut(s)	211, 2274
BspQI	GCTCTTC	2 cut(s)	1372, 1887
BspT107I	GGYRCC	2 cut(s)	331, 672
BsrBI	CCGCTC	2 cut(s)	2143, 2450
BsrGI	TGTACA	1 cut(s)	1046
BsrI	ACTGG	1 cut(s)	578
BssECI	CCNNGG	8 cut(s)	188, 236, 583, 741, 786, 1799, 2311, 2459
BssMI	GATC	9 cut(s)	203, 984, 1738, 1886, 2266, 2286, 2293, 2492, 2581
BssNI	GRCGYC	1 cut(s)	1796
BssSI	CACGAG	3 cut(s)	164, 2205, 2529
BssT1I	CCWWGG	5 cut(s)	583, 741, 786, 1799, 2311
Bst2BI	CACGAG	3 cut(s)	164, 2205, 2529
Bst2UI	CCWGG	7 cut(s)	237, 308, 429, 435, 1092, 2092, 2460
Bst6I	CTCTTC	3 cut(s)	1372, 1887, 1926
BstACI	GRCGYC	1 cut(s)	1796
BstAPI	GCANNNNNTGC	1 cut(s)	2084
BstAUI	TGTACA	1 cut(s)	1046
BstC8I	GCNNGC	6 cut(s)	1054, 1058, 1384, 1705, 1869, 1878
BstDEI	CTNAG	7 cut(s)	35, 990, 1829, 2007, 2300, 2489, 2542
BstDSI	CCRYGG	3 cut(s)	583, 741, 1799
BstF5I	GGATG	6 cut(s)	375, 633, 751, 928, 1219, 1501
BstFNI	CGCG	1 cut(s)	2406
BstH2I	RGCGCY	2 cut(s)	1230, 2709
BstHHI	GCGC	2 cut(s)	1229, 2708
BstKTI	GATC	9 cut(s)	206, 987, 1741, 1889, 2269, 2289, 2296, 2495, 2584
BstMBI	GATC	9 cut(s)	203, 984, 1738, 1886, 2266, 2286, 2293, 2492, 2581
BstMWI	GCNNNNNNNGC	7 cut(s)	1512, 1873, 1877, 2084, 2210, 2403, 2654
BstNI	CCWGG	7 cut(s)	237, 308, 429, 435, 1092, 2092, 2460
BstNSI	RCATGY	2 cut(s)	450, 1056
BstSCI	CCNGG	8 cut(s)	235, 306, 427, 433, 1090, 2090, 2329, 2458
BstSFI	CTRYAG	4 cut(s)	723, 1848, 2199, 2735
BstSLI	GKGCMC	2 cut(s)	2349, 2482
BstUI	CGCG	1 cut(s)	2406
BstV1I	GCAGC	3 cut(s)	468, 1527, 2074
BstV2I	GAAGAC	3 cut(s)	464, 964, 2679
Bsu15I	ATCGAT	1 cut(s)	1364
BsuRI	GGCC	1 cut(s)	1599
BsuTUI	ATCGAT	1 cut(s)	1364
BtgI	CCRYGG	3 cut(s)	583, 741, 1799
BtrI	CACGTC	1 cut(s)	2362
BtsCI	GGATG	6 cut(s)	375, 633, 751, 928, 1219, 1501
BtsI	GCAGTG	1 cut(s)	1741
BtsIMutI	CAGTG	4 cut(s)	987, 1174, 1741, 2263
Cac8I	GCNNGC	6 cut(s)	1054, 1058, 1384, 1705, 1869, 1878
CaiI	CAGNNNCTG	3 cut(s)	313, 481, 1075
CciI	TCATGA	1 cut(s)	148
CfoI	GCGC	2 cut(s)	1229, 2708
Cfr13I	GGNCC	1 cut(s)	431
ClaI	ATCGAT	1 cut(s)	1364
CseI	GACGC	1 cut(s)	1804
CsiI	ACCWGGT	1 cut(s)	433
Csp6I	GTAC	4 cut(s)	673, 1047, 1791, 2196
CviQI	GTAC	4 cut(s)	673, 1047, 1791, 2196
DdeI	CTNAG	7 cut(s)	35, 990, 1829, 2007, 2300, 2489, 2542
DpnI	GATC	9 cut(s)	205, 986, 1740, 1888, 2268, 2288, 2295, 2494, 2583
DpnII	GATC	9 cut(s)	203, 984, 1738, 1886, 2266, 2286, 2293, 2492, 2581
DraI	TTTAAA	1 cut(s)	1438
DraIII	CACNNNGTG	1 cut(s)	15
Eam1104I	CTCTTC	3 cut(s)	1372, 1887, 1926
EarI	CTCTTC	3 cut(s)	1372, 1887, 1926
Ecl136II	GAGCTC	2 cut(s)	34, 1561
Eco130I	CCWWGG	5 cut(s)	583, 741, 786, 1799, 2311
Eco147I	AGGCCT	1 cut(s)	1599
Eco24I	GRGCYC	3 cut(s)	36, 1563, 2331
Eco32I	GATATC	2 cut(s)	499, 1097
Eco47I	GGWCC	1 cut(s)	431
Eco47III	AGCGCT	2 cut(s)	1228, 2707
Eco53kI	GAGCTC	2 cut(s)	34, 1561
Eco57I	CTGAAG	3 cut(s)	458, 1265, 2691
EcoICRI	GAGCTC	2 cut(s)	34, 1561
EcoRI	GAATTC	1 cut(s)	1006
EcoRII	CCWGG	7 cut(s)	235, 306, 427, 433, 1090, 2090, 2458
EcoRV	GATATC	2 cut(s)	499, 1097
EcoT14I	CCWWGG	5 cut(s)	583, 741, 786, 1799, 2311
EcoT22I	ATGCAT	1 cut(s)	897
EcoT38I	GRGCYC	3 cut(s)	36, 1563, 2331
ErhI	CCWWGG	5 cut(s)	583, 741, 786, 1799, 2311
FalI	AAGNNNNNCTT	2 cut(s)	1524, 1556
FauI	CCCGC	2 cut(s)	2399, 2630
FauNDI	CATATG	4 cut(s)	208, 467, 2083, 2371
FbaI	TGATCA	1 cut(s)	1738
FblI	GTMKAC	1 cut(s)	173
Fnu4HI	GCNGC	4 cut(s)	482, 1516, 2088, 2451
FokI	GGATG	6 cut(s)	362, 620, 758, 915, 1206, 1508
FriOI	GRGCYC	3 cut(s)	36, 1563, 2331
Fsp4HI	GCNGC	4 cut(s)	482, 1516, 2088, 2451
FspBI	CTAG	3 cut(s)	83, 1146, 2219
GlaI	GCGC	2 cut(s)	1228, 2707
GluI	GCNGC	4 cut(s)	482, 1516, 2088, 2451
GsaI	CCCAGC	2 cut(s)	496, 1072
GsuI	CTGGAG	1 cut(s)	1680
HaeII	RGCGCY	2 cut(s)	1230, 2709
HaeIII	GGCC	1 cut(s)	1599
HapII	CCGG	2 cut(s)	2331, 2711
HgaI	GACGC	1 cut(s)	1804
HhaI	GCGC	2 cut(s)	1229, 2708
Hin1I	GRCGYC	1 cut(s)	1796
Hin6I	GCGC	2 cut(s)	1227, 2706
HinP1I	GCGC	2 cut(s)	1227, 2706
HincII	GTYRAC	1 cut(s)	2437
HindII	GTYRAC	1 cut(s)	2437
HindIII	AAGCTT	3 cut(s)	1823, 1839, 2625
HinfI	GANTC	9 cut(s)	86, 650, 657, 698, 1693, 2036, 2366, 2510, 2535
HpaII	CCGG	2 cut(s)	2331, 2711
HphI	GGTGA	6 cut(s)	417, 1874, 1991, 2048, 2404, 2633
Hpy166II	GTNNAC	6 cut(s)	174, 565, 1080, 2437, 2480, 2528
Hpy8I	GTNNAC	6 cut(s)	174, 565, 1080, 2437, 2480, 2528
Hpy99I	CGWCG	1 cut(s)	1798
HpyAV	CCTTC	9 cut(s)	97, 118, 648, 1462, 1526, 1589, 1610, 2063, 2069
HpyCH4IV	ACGT	1 cut(s)	2361
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1512, 1873, 1877, 2084, 2210, 2403, 2654
HpyF3I	CTNAG	7 cut(s)	35, 990, 1829, 2007, 2300, 2489, 2542
HpySE526I	ACGT	1 cut(s)	2361
Hsp92I	GRCGYC	1 cut(s)	1796
HspAI	GCGC	2 cut(s)	1227, 2706
KpnI	GGTACC	1 cut(s)	676
Ksp22I	TGATCA	1 cut(s)	1738
Kzo9I	GATC	9 cut(s)	203, 984, 1738, 1886, 2266, 2286, 2293, 2492, 2581
LguI	GCTCTTC	2 cut(s)	1372, 1887
LmnI	GCTCC	8 cut(s)	310, 1292, 1558, 1566, 1751, 2552, 2645, 2713
Lsp1109I	GCAGC	3 cut(s)	468, 1527, 2074
MabI	ACCWGGT	1 cut(s)	433
MaeI	CTAG	3 cut(s)	83, 1146, 2219
MaeII	ACGT	1 cut(s)	2361
MaeIII	GTNAC	4 cut(s)	644, 1175, 1574, 2722
MalI	GATC	9 cut(s)	205, 986, 1740, 1888, 2268, 2288, 2295, 2494, 2583
MbiI	CCGCTC	2 cut(s)	2143, 2450
MboI	GATC	9 cut(s)	203, 984, 1738, 1886, 2266, 2286, 2293, 2492, 2581
MfeI	CAATTG	1 cut(s)	1427
MhlI	GDGCHC	8 cut(s)	36, 609, 1384, 1563, 2331, 2349, 2399, 2482
MlyI	GAGTC	3 cut(s)	95, 1687, 2504
MmeI	TCCRAC	3 cut(s)	1332, 1785, 2136
Mph1103I	ATGCAT	1 cut(s)	897
MroXI	GAANNNNTTC	5 cut(s)	702, 717, 835, 1536, 1599
MslI	CAYNNNNRTG	2 cut(s)	915, 2619
MspI	CCGG	2 cut(s)	2331, 2711
MspR9I	CCNGG	8 cut(s)	237, 308, 429, 435, 1092, 2092, 2331, 2460
MunI	CAATTG	1 cut(s)	1427
Mva1269I	GAATGC	2 cut(s)	895, 1787
MvaI	CCWGG	7 cut(s)	237, 308, 429, 435, 1092, 2092, 2460
MvnI	CGCG	1 cut(s)	2406
MwoI	GCNNNNNNNGC	7 cut(s)	1512, 1873, 1877, 2084, 2210, 2403, 2654
NciI	CCSGG	1 cut(s)	2331
NcoI	CCATGG	3 cut(s)	583, 741, 1799
NdeI	CATATG	4 cut(s)	208, 467, 2083, 2371
NdeII	GATC	9 cut(s)	203, 984, 1738, 1886, 2266, 2286, 2293, 2492, 2581
NlaIV	GGNNCC	5 cut(s)	234, 333, 674, 2548, 2647
NmuCI	GTSAC	1 cut(s)	644
NsiI	ATGCAT	1 cut(s)	897
NspI	RCATGY	2 cut(s)	450, 1056
PaeI	GCATGC	1 cut(s)	1056
PagI	TCATGA	1 cut(s)	148
PceI	AGGCCT	1 cut(s)	1599
PciI	ACATGT	1 cut(s)	446
PciSI	GCTCTTC	2 cut(s)	1372, 1887
PcsI	WCGNNNNNNNCGW	1 cut(s)	2682
PctI	GAATGC	2 cut(s)	895, 1787
PdmI	GAANNNNTTC	5 cut(s)	702, 717, 835, 1536, 1599
PfeI	GAWTC	6 cut(s)	650, 657, 698, 2036, 2366, 2535
PflMI	CCANNNNNTGG	3 cut(s)	584, 1229, 2356
PfoI	TCCNGGA	1 cut(s)	1090
PkrI	GCNGC	4 cut(s)	483, 1517, 2089, 2452
PleI	GAGTC	3 cut(s)	94, 1687, 2504
PpsI	GAGTC	3 cut(s)	94, 1687, 2504
PscI	ACATGT	1 cut(s)	446
PshBI	ATTAAT	1 cut(s)	324
Psp124BI	GAGCTC	2 cut(s)	36, 1563
Psp6I	CCWGG	7 cut(s)	235, 306, 427, 433, 1090, 2090, 2458
PspFI	CCCAGC	2 cut(s)	492, 1068
PspGI	CCWGG	7 cut(s)	235, 306, 427, 433, 1090, 2090, 2458
PspN4I	GGNNCC	5 cut(s)	234, 333, 674, 2548, 2647
PspPI	GGNCC	1 cut(s)	431
PstNI	CAGNNNCTG	3 cut(s)	313, 481, 1075
RsaI	GTAC	4 cut(s)	674, 1048, 1792, 2197
RsaNI	GTAC	4 cut(s)	673, 1047, 1791, 2196
RseI	CAYNNNNRTG	2 cut(s)	915, 2619
SacI	GAGCTC	2 cut(s)	36, 1563
SapI	GCTCTTC	2 cut(s)	1372, 1887
SatI	GCNGC	4 cut(s)	482, 1516, 2088, 2451
Sau3AI	GATC	9 cut(s)	203, 984, 1738, 1886, 2266, 2286, 2293, 2492, 2581
Sau96I	GGNCC	1 cut(s)	431
SchI	GAGTC	3 cut(s)	95, 1687, 2504
ScrFI	CCNGG	8 cut(s)	237, 308, 429, 435, 1092, 2092, 2331, 2460
SduI	GDGCHC	8 cut(s)	36, 609, 1384, 1563, 2331, 2349, 2399, 2482
SexAI	ACCWGGT	1 cut(s)	433
SfcI	CTRYAG	4 cut(s)	723, 1848, 2199, 2735
SinI	GGWCC	1 cut(s)	431
SmiMI	CAYNNNNRTG	2 cut(s)	915, 2619
SmlI	CTYRAG	3 cut(s)	135, 227, 1500
SmoI	CTYRAG	3 cut(s)	135, 227, 1500
SphI	GCATGC	1 cut(s)	1056
SseBI	AGGCCT	1 cut(s)	1599
SsiI	CCGC	7 cut(s)	349, 589, 2143, 2406, 2416, 2450, 2637
SspI	AATATT	1 cut(s)	1303
SspMI	CTAG	3 cut(s)	83, 1146, 2219
SstI	GAGCTC	2 cut(s)	36, 1563
StuI	AGGCCT	1 cut(s)	1599
StyD4I	CCNGG	8 cut(s)	235, 306, 427, 433, 1090, 2090, 2329, 2458
StyI	CCWWGG	5 cut(s)	583, 741, 786, 1799, 2311
TaiI	ACGT	1 cut(s)	2364
TaqI	TCGA	8 cut(s)	762, 1200, 1364, 1885, 1950, 2062, 2517, 2580
TatI	WGTACW	1 cut(s)	1046
TauI	GCSGC	1 cut(s)	2453
TfiI	GAWTC	6 cut(s)	650, 657, 698, 2036, 2366, 2535
TscAI	CASTG	4 cut(s)	994, 1174, 1748, 2263
TseFI	GTSAC	1 cut(s)	644
TseI	GCWGC	3 cut(s)	481, 1515, 2087
Tsp45I	GTSAC	1 cut(s)	644
TspRI	CASTG	4 cut(s)	994, 1174, 1748, 2263
Van91I	CCANNNNNTGG	3 cut(s)	584, 1229, 2356
VneI	GTGCAC	1 cut(s)	2478
VpaK11BI	GGWCC	1 cut(s)	431
VspI	ATTAAT	1 cut(s)	324
XapI	RAATTY	7 cut(s)	4, 251, 973, 1006, 1036, 1262, 2729
XbaI	TCTAGA	1 cut(s)	1145
XceI	RCATGY	2 cut(s)	450, 1056
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2088
XmiI	GTMKAC	1 cut(s)	173
XmnI	GAANNNNTTC	5 cut(s)	702, 717, 835, 1536, 1599
XspI	CTAG	3 cut(s)	83, 1146, 2219
Zsp2I	ATGCAT	1 cut(s)	897

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.