FvH4_7g20020

Assembly, mitochondrial proton-transport ATP synth complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012541
Physical Location & Seq
Forward (+)
16366810 .. 16369192
2383 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g20020.t1

Sequence Viewer

Length: 729 bp
ATGGCGAGGACGGCTCTCTTTCACTTGGTCCGATCACACTCTAAACGCCTCGCAACTCCAAATACCCTTTCAGGATATCAGCCTTGCGAGTCTGGAGCATGGGCACGTGGCCTGAACACCAAACCTAGTTTCAACTCTGGTGTTGGGATCAATGGTTTCCAGAAGAGATGGGCGTCTCAGGCTGCTATAAAAGAAGATGATGGCAAAATCAGCATCGGACCCCGTAGAGGTGTCGAAACTGGGAAAGATGAAAAAGATTCTGGGATTGTTTACTATGGTCCAATCTCATCTACTATCAAGAAAGTGAAACTTCTATCGCTTTCAACCTGCTGCCTTTCTGTATCGTTAGGCCCTGTGATAACCTTTATGACATCACCTGATATGAATGTTATCTTGAAGGGTGCCGTGGCATCTTCAGTGATATTCTTAAGTGCGTCTACCACAGCTGCCCTTCACTGGTTTGTGACCCCATACATACACAAGCTCAAGTGGAAGCCTGGTTCAGACAGTTTTGAGGTCGAAATGTTGTCATGGCTAGCAACTTACATACCAAGGACAATTAAGTTTGCTGATATCAGGCCACCAGAAACCCAGAGGCCTTTTGTGACTTTCAAGGCAAACGGGAAGTTCTACTTCGTTGATGCTGATCATTGTCAGAACAAGGCTTTGTTAGCAAAGCTGACCCCGCAGAAAGCACCAGCTCGTGAATCCGCTTTTAAGAACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM70

Protein Analysis

243

Amino Acids

26.6

Weight (kDa)

9.8

Isoelectric Point (pI)

25.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM70 PF06979 89 - 226 1.6e-11 TMEM70/TMEM186/TMEM223 protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012541)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35790
fragaria_vesca FvH4_7g20020
malus_domestica MD01G1111700.v1.1 MD07G1177900.v1.1
prunus_persica Prupe.2G217200_v2.0.a1 Prupe.2G217200_v2.0.a1
pyrus_communis pycom01g13890 pycom07g17170 pycom07g17180
rosa_chinensis RchiOBHm_Chr1g0363981
rosa_laevigata RLG00000027531
rosa_multiflora Rmu_sc0001607.1_g000014 Rmu_ssc0000167.1_g000025
rosa_roxburghii Rroxscaffold_4G00292790
rosa_rugosa Rorug01G0311700 Rorug01G0311800
rosa_samantha Rh1BG282700 Rh1DG314100
rosa_wichuraiana Rw1G028330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 401
AccI GTMKAC 1 cut(s) 437
AciI CCGC 2 cut(s) 686, 711
AclWI GGATC 1 cut(s) 155
AcuI CTGAAG 1 cut(s) 399
AcvI CACGTG 1 cut(s) 107
AcyI GRCGYC 1 cut(s) 173
AfiI CCNNNNNNNGG 2 cut(s) 227, 456
AflII CTTAAG 1 cut(s) 427
AgsI TTSAA 4 cut(s) 133, 324, 397, 613
AjnI CCWGG 1 cut(s) 496
AjuI GAANNNNNNNTTGG 4 cut(s) 52, 84, 113, 145
AloI GAACNNNNNNTCC 2 cut(s) 484, 516
AluBI AGCT 4 cut(s) 446, 484, 679, 701
AluI AGCT 4 cut(s) 446, 484, 679, 701
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 1 cut(s) 155
AoxI GGCC 4 cut(s) 109, 349, 578, 596
ApeKI GCWGC 3 cut(s) 182, 330, 446
AspS9I GGNCC 4 cut(s) 28, 218, 278, 350
AsuHPI GGTGA 1 cut(s) 366
AsuNHI GCTAGC 1 cut(s) 535
AvaII GGWCC 3 cut(s) 28, 218, 278
BaeGI GKGCMC 1 cut(s) 106
BanI GGYRCC 1 cut(s) 401
BauI CACGAG 1 cut(s) 702
BbrPI CACGTG 1 cut(s) 107
BbvI GCAGC 3 cut(s) 169, 317, 433
BccI CCATC 2 cut(s) 162, 194
BceAI ACGGC 2 cut(s) 27, 389
BciT130I CCWGG 1 cut(s) 498
BclI TGATCA 1 cut(s) 646
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 2 cut(s) 126, 536
BfrI CTTAAG 1 cut(s) 427
BfuAI ACCTGC 1 cut(s) 335
BisI GCNGC 3 cut(s) 183, 331, 447
BlsI GCNGC 3 cut(s) 184, 332, 448
Bme1390I CCNGG 1 cut(s) 498
Bme18I GGWCC 3 cut(s) 28, 218, 278
BmgT120I GGNCC 4 cut(s) 28, 218, 278, 350
BmiI GGNNCC 2 cut(s) 220, 403
BmrFI CCNGG 1 cut(s) 498
BmrI ACTGGG 1 cut(s) 249
BmsI GCATC 3 cut(s) 222, 419, 631
BmtI GCTAGC 1 cut(s) 539
BmuI ACTGGG 1 cut(s) 249
BplI GAGNNNNNCTC 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 470
BsaAI YACGTR 1 cut(s) 107
BsaBI GATNNNNATC 1 cut(s) 645
BsaHI GRCGYC 1 cut(s) 173
BsaJI CCNNGG 2 cut(s) 405, 551
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 456
Bse1I ACTGG 2 cut(s) 244, 461
Bse8I GATNNNNATC 1 cut(s) 645
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 2 cut(s) 405, 551
BseJI GATNNNNATC 1 cut(s) 645
BseLI CCNNNNNNNGG 2 cut(s) 227, 456
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 2 cut(s) 244, 461
BseSI GKGCMC 1 cut(s) 106
BseXI GCAGC 3 cut(s) 169, 317, 433
BshFI GGCC 4 cut(s) 111, 351, 580, 598
BshNI GGYRCC 1 cut(s) 401
BslI CCNNNNNNNGG 2 cut(s) 227, 456
BsmAI GTCTC 1 cut(s) 180
BsmBI CGTCTC 1 cut(s) 180
BsnI GGCC 4 cut(s) 111, 351, 580, 598
Bsp1286I GDGCHC 1 cut(s) 106
Bsp143I GATC 3 cut(s) 32, 147, 646
BspACI CCGC 2 cut(s) 686, 711
BspANI GGCC 4 cut(s) 111, 351, 580, 598
BspCNI CTCAG 1 cut(s) 190
BspLI GGNNCC 2 cut(s) 220, 403
BspMI ACCTGC 1 cut(s) 335
BspOI GCTAGC 1 cut(s) 539
BspPI GGATC 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 401
BspTI CTTAAG 1 cut(s) 427
BsrI ACTGG 2 cut(s) 244, 461
BssECI CCNNGG 2 cut(s) 405, 551
BssMI GATC 3 cut(s) 32, 147, 646
BssNI GRCGYC 1 cut(s) 173
BssSI CACGAG 1 cut(s) 702
BssT1I CCWWGG 1 cut(s) 551
Bst2BI CACGAG 1 cut(s) 702
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 1 cut(s) 509
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 173
BstAFI CTTAAG 1 cut(s) 427
BstBAI YACGTR 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 1 cut(s) 177
BstDSI CCRYGG 1 cut(s) 405
BstKTI GATC 3 cut(s) 35, 150, 649
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 3 cut(s) 32, 147, 646
BstMWI GCNNNNNNNGC 5 cut(s) 11, 179, 210, 671, 685
BstNI CCWGG 1 cut(s) 498
BstSCI CCNGG 1 cut(s) 496
BstSLI GKGCMC 1 cut(s) 106
BstV1I GCAGC 3 cut(s) 169, 317, 433
BsuRI GGCC 4 cut(s) 111, 351, 580, 598
BtgI CCRYGG 1 cut(s) 405
BtsIMutI CAGTG 2 cut(s) 423, 454
BveI ACCTGC 1 cut(s) 335
Cac8I GCNNGC 1 cut(s) 537
Cfr13I GGNCC 4 cut(s) 28, 218, 278, 350
CseI GACGC 2 cut(s) 162, 423
CviAII CATG 2 cut(s) 99, 531
DdeI CTNAG 1 cut(s) 177
DpnI GATC 3 cut(s) 34, 149, 648
DpnII GATC 3 cut(s) 32, 147, 646
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 551
Eco147I AGGCCT 1 cut(s) 598
Eco32I GATATC 2 cut(s) 77, 574
Eco47I GGWCC 3 cut(s) 28, 218, 278
Eco57I CTGAAG 1 cut(s) 399
Eco72I CACGTG 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 496
EcoRV GATATC 2 cut(s) 77, 574
EcoT14I CCWWGG 1 cut(s) 551
ErhI CCWWGG 1 cut(s) 551
Esp3I CGTCTC 1 cut(s) 180
FaeI CATG 2 cut(s) 102, 534
FaiI YATR 9 cut(s) 100, 188, 276, 368, 383, 472, 476, 532, 548
FalI AAGNNNNNCTT 4 cut(s) 294, 326, 617, 649
FatI CATG 2 cut(s) 98, 530
FauI CCCGC 1 cut(s) 693
FbaI TGATCA 1 cut(s) 646
FblI GTMKAC 1 cut(s) 437
Fnu4HI GCNGC 3 cut(s) 183, 331, 447
Fsp4HI GCNGC 3 cut(s) 183, 331, 447
FspBI CTAG 2 cut(s) 126, 536
GluI GCNGC 3 cut(s) 183, 331, 447
GsuI CTGGAG 1 cut(s) 114
HaeIII GGCC 4 cut(s) 111, 351, 580, 598
HgaI GACGC 2 cut(s) 162, 423
Hin1I GRCGYC 1 cut(s) 173
Hin1II CATG 2 cut(s) 102, 534
HinfI GANTC 3 cut(s) 89, 257, 707
HphI GGTGA 1 cut(s) 366
Hpy166II GTNNAC 2 cut(s) 271, 438
Hpy188I TCNGA 4 cut(s) 32, 218, 505, 657
Hpy188III TCNNGA 6 cut(s) 72, 93, 160, 298, 394, 704
Hpy8I GTNNAC 2 cut(s) 271, 438
HpyAV CCTTC 2 cut(s) 391, 461
HpyCH4III ACNGT 1 cut(s) 509
HpyCH4IV ACGT 1 cut(s) 106
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 179, 210, 671, 685
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 1 cut(s) 106
Hsp92I GRCGYC 1 cut(s) 173
Hsp92II CATG 2 cut(s) 102, 534
Ksp22I TGATCA 1 cut(s) 646
Kzo9I GATC 3 cut(s) 32, 147, 646
LmnI GCTCC 1 cut(s) 95
Lsp1109I GCAGC 3 cut(s) 169, 317, 433
LweI GCATC 3 cut(s) 222, 419, 631
MaeI CTAG 2 cut(s) 126, 536
MaeII ACGT 1 cut(s) 106
MaeIII GTNAC 2 cut(s) 463, 604
MalI GATC 3 cut(s) 34, 149, 648
MboI GATC 3 cut(s) 32, 147, 646
MboII GAAGA 3 cut(s) 175, 206, 405
MhlI GDGCHC 1 cut(s) 106
MluCI AATT 1 cut(s) 558
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 4 cut(s) 59, 221, 508, 588
MseI TTAA 3 cut(s) 428, 561, 717
MspA1I CMGCKG 1 cut(s) 446
MspCI CTTAAG 1 cut(s) 427
MspR9I CCNGG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 498
MwoI GCNNNNNNNGC 5 cut(s) 11, 179, 210, 671, 685
NdeII GATC 3 cut(s) 32, 147, 646
NheI GCTAGC 1 cut(s) 535
NlaIII CATG 2 cut(s) 102, 534
NlaIV GGNNCC 2 cut(s) 220, 403
NmuCI GTSAC 2 cut(s) 463, 604
PceI AGGCCT 1 cut(s) 598
PfeI GAWTC 2 cut(s) 257, 707
PkrI GCNGC 3 cut(s) 184, 332, 448
PleI GAGTC 1 cut(s) 97
PmaCI CACGTG 1 cut(s) 107
PmlI CACGTG 1 cut(s) 107
PpsI GAGTC 1 cut(s) 97
Ppu21I YACGTR 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 496
PspCI CACGTG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 2 cut(s) 220, 403
PspPI GGNCC 4 cut(s) 28, 218, 278, 350
PvuII CAGCTG 1 cut(s) 446
SaqAI TTAA 3 cut(s) 428, 561, 717
SatI GCNGC 3 cut(s) 183, 331, 447
Sau3AI GATC 3 cut(s) 32, 147, 646
Sau96I GGNCC 4 cut(s) 28, 218, 278, 350
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 498
SduI GDGCHC 1 cut(s) 106
SfaNI GCATC 3 cut(s) 222, 419, 631
SinI GGWCC 3 cut(s) 28, 218, 278
SmlI CTYRAG 2 cut(s) 427, 485
SmoI CTYRAG 2 cut(s) 427, 485
Sse9I AATT 1 cut(s) 558
SseBI AGGCCT 1 cut(s) 598
SsiI CCGC 2 cut(s) 686, 711
SspMI CTAG 2 cut(s) 126, 536
StuI AGGCCT 1 cut(s) 598
StyD4I CCNGG 1 cut(s) 496
StyI CCWWGG 1 cut(s) 551
TaaI ACNGT 1 cut(s) 509
TaiI ACGT 1 cut(s) 109
TaqI TCGA 2 cut(s) 234, 519
TasI AATT 1 cut(s) 558
TfiI GAWTC 2 cut(s) 257, 707
Tru1I TTAA 3 cut(s) 428, 561, 717
Tru9I TTAA 3 cut(s) 428, 561, 717
TscAI CASTG 2 cut(s) 423, 461
TseFI GTSAC 2 cut(s) 463, 604
TseI GCWGC 3 cut(s) 182, 330, 446
Tsp45I GTSAC 2 cut(s) 463, 604
TspDTI ATGAA 2 cut(s) 264, 398
TspRI CASTG 2 cut(s) 423, 461
Vha464I CTTAAG 1 cut(s) 427
VpaK11BI GGWCC 3 cut(s) 28, 218, 278
XmiI GTMKAC 1 cut(s) 437
XspI CTAG 2 cut(s) 126, 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.