MD07G1177900.v1.1

Assembly, mitochondrial proton-transport ATP synth complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012541
Physical Location & Seq
Forward (+)
25724193 .. 25726537
2345 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1177900.v1.1.491

Sequence Viewer

Length: 726 bp
ATGACGAGGGCGGCTCTCTTTCAGTTGCTGCGATCACACTCCAGACAACTCACACCCTCAAACCTCCGCTCAGGTTATCAGCCATGCAGGTCTGCAGCATGGGCTGCTGGTCTGAACACTAAACTAAGTTTCCACTCTGGCTTCGAGATCAATGCTTCACAGAAAAGATGGGCATCTCATGCCACAACAAGAGAAGATGATGGCAAAATCAGCATTGGACCGCGTAGGGGTGGTCAAGTTGGGGAAGATGAAAAGGACTCAGGGGTCGTTTATTATGGCCCAATCTCATCTACCATCAAGAAAGTGAAACTTCTCTCGCTGTCGACCTGCTGCCTTTCTGTATCTCTGGGCCCTGTGATAACCTTTATGACATCTCCTGATATGAATGTGATCCTGAAGGGTGCGGTGGCATCTTCAGTGATATTCTTGAGTGCTTCTACCACAGCCGCCCTTCACTGGTTCGTGACCCCATACATCCACAAGCTTAGGTGGAAGCCTGGTTCAGACAGTTTTGAGGTGGAAATGATGTCATGGCTTGCAACATTCATCCCGAGGACTATTAAGTTTTCTGATATCAAGCCAGCAGAAACTAATCGGCCTTTTGTGACTTTTAAGGCCAATGGAAACTTTTACTTTGTCGACGCTGATCATTGTCATAACAAGGCTTTGTTAGCAAGACTGACCCCGCAGAAAGCAACTCATGAATCGGCTTTTAAGAATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM70

Protein Analysis

242

Amino Acids

26.66

Weight (kDa)

9.75

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM70 PF06979 89 - 226 1.7e-11 TMEM70/TMEM186/TMEM223 protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012541)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35790
fragaria_vesca FvH4_7g20020
malus_domestica MD01G1111700.v1.1 MD07G1177900.v1.1
prunus_persica Prupe.2G217200_v2.0.a1 Prupe.2G217200_v2.0.a1
pyrus_communis pycom01g13890 pycom07g17170 pycom07g17180
rosa_chinensis RchiOBHm_Chr1g0363981
rosa_laevigata RLG00000027531
rosa_multiflora Rmu_sc0001607.1_g000014 Rmu_ssc0000167.1_g000025
rosa_roxburghii Rroxscaffold_4G00292790
rosa_rugosa Rorug01G0311700 Rorug01G0311800
rosa_samantha Rh1BG282700 Rh1DG314100
rosa_wichuraiana Rw1G028330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
Acc36I ACCTGC 2 cut(s) 78, 335
AccBSI CCGCTC 1 cut(s) 69
AccI GTMKAC 2 cut(s) 323, 639
AccII CGCG 1 cut(s) 223
AciI CCGC 6 cut(s) 11, 67, 221, 404, 447, 686
AclWI GGATC 1 cut(s) 385
AcsI RAATTY 1 cut(s) 718
AcuI CTGAAG 2 cut(s) 399, 416
AfiI CCNNNNNNNGG 2 cut(s) 227, 456
AjnI CCWGG 1 cut(s) 496
AloI GAACNNNNNNTCC 2 cut(s) 484, 516
AluBI AGCT 1 cut(s) 484
AluI AGCT 1 cut(s) 484
AlwI GGATC 1 cut(s) 385
AlwNI CAGNNNCTG 1 cut(s) 28
Ama87I CYCGRG 1 cut(s) 550
AoxI GGCC 4 cut(s) 277, 349, 596, 615
ApaI GGGCCC 1 cut(s) 353
ApeKI GCWGC 4 cut(s) 28, 95, 104, 330
ApoI RAATTY 1 cut(s) 718
AspS9I GGNCC 4 cut(s) 218, 278, 349, 350
AvaI CYCGRG 1 cut(s) 550
AvaII GGWCC 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 353
BanII GRGCYC 1 cut(s) 353
BbvI GCAGC 4 cut(s) 15, 91, 107, 317
BccI CCATC 3 cut(s) 162, 194, 302
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 1 cut(s) 498
BclI TGATCA 1 cut(s) 646
BfmI CTRYAG 1 cut(s) 93
BfuAI ACCTGC 2 cut(s) 78, 335
BisI GCNGC 6 cut(s) 12, 29, 96, 105, 331, 447
BlsI GCNGC 6 cut(s) 13, 30, 97, 106, 332, 448
Bme1390I CCNGG 1 cut(s) 498
Bme18I GGWCC 1 cut(s) 218
BmeT110I CYCGRG 1 cut(s) 550
BmgT120I GGNCC 4 cut(s) 218, 278, 349, 350
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 1 cut(s) 498
BmsI GCATC 2 cut(s) 182, 419
BplI GAGNNNNNCTC 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 25
Bpu10I CCTNAGC 2 cut(s) 70, 485
BpuEI CTTGAG 1 cut(s) 448
BsaBI GATNNNNATC 1 cut(s) 172
BsaJI CCNNGG 1 cut(s) 551
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 456
Bse1I ACTGG 1 cut(s) 461
Bse8I GATNNNNATC 1 cut(s) 172
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 1 cut(s) 551
BseGI GGATG 2 cut(s) 474, 546
BseJI GATNNNNATC 1 cut(s) 172
BseLI CCNNNNNNNGG 2 cut(s) 227, 456
BseMII CTCAG 2 cut(s) 84, 273
BseNI ACTGG 1 cut(s) 461
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 4 cut(s) 15, 91, 107, 317
Bsh1236I CGCG 1 cut(s) 223
BshFI GGCC 4 cut(s) 279, 351, 598, 617
BsiHKCI CYCGRG 1 cut(s) 550
BslI CCNNNNNNNGG 2 cut(s) 227, 456
BsnI GGCC 4 cut(s) 279, 351, 598, 617
BsoBI CYCGRG 1 cut(s) 550
Bsp120I GGGCCC 1 cut(s) 349
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 4 cut(s) 32, 147, 390, 646
BspACI CCGC 6 cut(s) 11, 67, 221, 404, 447, 686
BspANI GGCC 4 cut(s) 279, 351, 598, 617
BspCNI CTCAG 2 cut(s) 83, 272
BspFNI CGCG 1 cut(s) 223
BspHI TCATGA 1 cut(s) 700
BspLI GGNNCC 1 cut(s) 351
BspMAI CTGCAG 1 cut(s) 97
BspMI ACCTGC 2 cut(s) 78, 335
BspPI GGATC 1 cut(s) 385
BsrBI CCGCTC 1 cut(s) 69
BsrI ACTGG 1 cut(s) 461
BssECI CCNNGG 1 cut(s) 551
BssMI GATC 4 cut(s) 32, 147, 390, 646
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 1 cut(s) 509
BstAPI GCANNNNNTGC 2 cut(s) 104, 179
BstC8I GCNNGC 2 cut(s) 537, 582
BstDEI CTNAG 4 cut(s) 70, 125, 259, 485
BstF5I GGATG 2 cut(s) 474, 546
BstFNI CGCG 1 cut(s) 223
BstKTI GATC 4 cut(s) 35, 150, 393, 649
BstMBI GATC 4 cut(s) 32, 147, 390, 646
BstMWI GCNNNNNNNGC 5 cut(s) 101, 104, 179, 210, 671
BstNI CCWGG 1 cut(s) 498
BstSCI CCNGG 1 cut(s) 496
BstSFI CTRYAG 1 cut(s) 93
BstSLI GKGCMC 1 cut(s) 353
BstUI CGCG 1 cut(s) 223
BstV1I GCAGC 4 cut(s) 15, 91, 107, 317
BsuRI GGCC 4 cut(s) 279, 351, 598, 617
BtsCI GGATG 2 cut(s) 474, 546
BtsIMutI CAGTG 2 cut(s) 423, 454
BveI ACCTGC 2 cut(s) 78, 335
Cac8I GCNNGC 2 cut(s) 537, 582
CaiI CAGNNNCTG 1 cut(s) 28
CciI TCATGA 1 cut(s) 700
Cfr13I GGNCC 4 cut(s) 218, 278, 349, 350
CseI GACGC 1 cut(s) 650
CviAII CATG 5 cut(s) 84, 99, 179, 531, 701
DdeI CTNAG 4 cut(s) 70, 125, 259, 485
DpnI GATC 4 cut(s) 34, 149, 392, 648
DpnII GATC 4 cut(s) 32, 147, 390, 646
DrdI GACNNNNNNGTC 1 cut(s) 263
DseDI GACNNNNNNGTC 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 353
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 218
Eco57I CTGAAG 2 cut(s) 399, 416
Eco88I CYCGRG 1 cut(s) 550
EcoO109I RGGNCCY 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 496
EcoRV GATATC 1 cut(s) 574
EcoT38I GRGCYC 1 cut(s) 353
FaeI CATG 5 cut(s) 87, 102, 182, 534, 704
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FatI CATG 5 cut(s) 83, 98, 178, 530, 700
FauI CCCGC 1 cut(s) 693
FbaI TGATCA 1 cut(s) 646
FblI GTMKAC 2 cut(s) 323, 639
Fnu4HI GCNGC 6 cut(s) 12, 29, 96, 105, 331, 447
FokI GGATG 2 cut(s) 461, 533
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 6 cut(s) 12, 29, 96, 105, 331, 447
GluI GCNGC 6 cut(s) 12, 29, 96, 105, 331, 447
GsuI CTGGAG 1 cut(s) 25
HaeIII GGCC 4 cut(s) 279, 351, 598, 617
HgaI GACGC 1 cut(s) 650
Hin1II CATG 5 cut(s) 87, 102, 182, 534, 704
HincII GTYRAC 2 cut(s) 324, 640
HindII GTYRAC 2 cut(s) 324, 640
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 2 cut(s) 257, 704
Hpy166II GTNNAC 2 cut(s) 324, 640
Hpy188I TCNGA 3 cut(s) 114, 505, 571
Hpy188III TCNNGA 9 cut(s) 42, 145, 298, 377, 394, 427, 463, 550, 701
Hpy8I GTNNAC 2 cut(s) 324, 640
Hpy99I CGWCG 1 cut(s) 644
HpyAV CCTTC 2 cut(s) 391, 461
HpyCH4III ACNGT 1 cut(s) 509
HpyCH4V TGCA 3 cut(s) 87, 95, 539
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 104, 179, 210, 671
HpyF3I CTNAG 4 cut(s) 70, 125, 259, 485
Hsp92II CATG 5 cut(s) 87, 102, 182, 534, 704
Ksp22I TGATCA 1 cut(s) 646
Kzo9I GATC 4 cut(s) 32, 147, 390, 646
Lsp1109I GCAGC 4 cut(s) 15, 91, 107, 317
LweI GCATC 2 cut(s) 182, 419
MaeIII GTNAC 2 cut(s) 463, 604
MalI GATC 4 cut(s) 34, 149, 392, 648
MbiI CCGCTC 1 cut(s) 69
MboI GATC 4 cut(s) 32, 147, 390, 646
MboII GAAGA 3 cut(s) 206, 257, 405
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 1 cut(s) 718
MlyI GAGTC 1 cut(s) 251
MnlI CCTC 4 cut(s) 67, 74, 508, 546
MseI TTAA 3 cut(s) 561, 612, 714
MspR9I CCNGG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 498
MvnI CGCG 1 cut(s) 223
MwoI GCNNNNNNNGC 5 cut(s) 101, 104, 179, 210, 671
NdeII GATC 4 cut(s) 32, 147, 390, 646
NlaIII CATG 5 cut(s) 87, 102, 182, 534, 704
NlaIV GGNNCC 1 cut(s) 351
NmuCI GTSAC 2 cut(s) 463, 604
PagI TCATGA 1 cut(s) 700
PfeI GAWTC 1 cut(s) 704
PkrI GCNGC 6 cut(s) 13, 30, 97, 106, 332, 448
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 496
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 1 cut(s) 351
PspOMI GGGCCC 1 cut(s) 349
PspPI GGNCC 4 cut(s) 218, 278, 349, 350
PstI CTGCAG 1 cut(s) 97
PstNI CAGNNNCTG 1 cut(s) 28
SalI GTCGAC 2 cut(s) 322, 638
SaqAI TTAA 3 cut(s) 561, 612, 714
SatI GCNGC 6 cut(s) 12, 29, 96, 105, 331, 447
Sau3AI GATC 4 cut(s) 32, 147, 390, 646
Sau96I GGNCC 4 cut(s) 218, 278, 349, 350
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 498
SduI GDGCHC 1 cut(s) 353
SetI ASST 8 cut(s) 66, 76, 92, 329, 365, 486, 491, 519
SfaNI GCATC 2 cut(s) 182, 419
SfcI CTRYAG 1 cut(s) 93
SinI GGWCC 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 427
SmoI CTYRAG 1 cut(s) 427
Sse9I AATT 1 cut(s) 718
SsiI CCGC 6 cut(s) 11, 67, 221, 404, 447, 686
StyD4I CCNGG 1 cut(s) 496
TaaI ACNGT 1 cut(s) 509
TaqI TCGA 3 cut(s) 144, 323, 639
TasI AATT 1 cut(s) 718
TauI GCSGC 2 cut(s) 14, 449
TfiI GAWTC 1 cut(s) 704
Tru1I TTAA 3 cut(s) 561, 612, 714
Tru9I TTAA 3 cut(s) 561, 612, 714
TscAI CASTG 2 cut(s) 423, 461
TseFI GTSAC 2 cut(s) 463, 604
TseI GCWGC 4 cut(s) 28, 95, 104, 330
Tsp45I GTSAC 2 cut(s) 463, 604
TspDTI ATGAA 4 cut(s) 264, 398, 535, 717
TspRI CASTG 2 cut(s) 423, 461
VpaK11BI GGWCC 1 cut(s) 218
XapI RAATTY 1 cut(s) 718
XmiI GTMKAC 2 cut(s) 323, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.