MD01G1111700.v1.1

Assembly, mitochondrial proton-transport ATP synth complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012541
Physical Location & Seq
Forward (+)
22423392 .. 22425675
2284 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1111700.v1.1.491

Sequence Viewer

Length: 726 bp
ATGACGAGAGCGGCTCTCCTTCAGTTGCTGCGATCACAGTCCAGACACCTCGCATCCTCAAGCTTCCGCTCAGGTTATCAGCCGTGCAGGTCTGCAGCATGGGCTGCTAGTCTGAACACTAAACTGGGTTTCAACTCTGGCTTCGAGATCAATGCTTCACAGAAACGATGGGCTTCTCGTGCCACAACAAGAGAAGATGATGGCAAAATCAGCATTGGACCACGCAGGGGTGGCGAAATTGGGGAAGATGAAAAGGACTCTGGGGTTGTTTACTATGGCCCAATCTCATCTACTATCAAGAAAGTGAAACTTCTCTCGCTGTCGACCTGCTGCCTTTCTGTATCTCTAGGCCCTGTGATAACCTTTATGACATCTCCTGGTATGAATGTGATCTTGAAGGGTGCAGTGGCATCTTCAGTGATATTCTTGAGTGCTTCTACCACAGCCGCTCTTCACTGGTTCGTGACCCCATACATTCACAAGCTCAAGTGGAAGCCTGGTTCAGACAGTTTTGAGGTGGAAATGATGTCATGGCTTGCAACATTCATTCCGAGGACTATTAAATTTTCTGATATCCGGCCAGCAGAAACCAACAGGCCTTTTGTGACTTTTAAGGCCAATGGAAACTTTTACTTCGTGGACGCAGATCATTGTCATAACAAGGCTTTGCTAGCAAGACTGACCCCGCAGAAAGAAACTCGTGAGTCGGCTTTTAAGAACTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM70

Protein Analysis

242

Amino Acids

26.62

Weight (kDa)

9.83

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM70 PF06979 89 - 226 3.3e-11 TMEM70/TMEM186/TMEM223 protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012541)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35790
fragaria_vesca FvH4_7g20020
malus_domestica MD01G1111700.v1.1 MD07G1177900.v1.1
prunus_persica Prupe.2G217200_v2.0.a1 Prupe.2G217200_v2.0.a1
pyrus_communis pycom01g13890 pycom07g17170 pycom07g17180
rosa_chinensis RchiOBHm_Chr1g0363981
rosa_laevigata RLG00000027531
rosa_multiflora Rmu_sc0001607.1_g000014 Rmu_ssc0000167.1_g000025
rosa_roxburghii Rroxscaffold_4G00292790
rosa_rugosa Rorug01G0311700 Rorug01G0311800
rosa_samantha Rh1BG282700 Rh1DG314100
rosa_wichuraiana Rw1G028330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 78, 335
AccBSI CCGCTC 3 cut(s) 11, 69, 449
AccI GTMKAC 1 cut(s) 323
AciI CCGC 4 cut(s) 11, 67, 447, 686
AcoI YGGCCR 1 cut(s) 578
AcsI RAATTY 1 cut(s) 563
AcuI CTGAAG 2 cut(s) 5, 399
AfiI CCNNNNNNNGG 1 cut(s) 227
AgsI TTSAA 2 cut(s) 133, 397
AjnI CCWGG 2 cut(s) 376, 496
AloI GAACNNNNNNTCC 2 cut(s) 484, 516
AluBI AGCT 2 cut(s) 63, 484
AluI AGCT 2 cut(s) 63, 484
AlwNI CAGNNNCTG 1 cut(s) 28
AoxI GGCC 5 cut(s) 277, 349, 578, 596, 615
ApeKI GCWGC 4 cut(s) 28, 95, 104, 330
ApoI RAATTY 1 cut(s) 563
AspS9I GGNCC 3 cut(s) 218, 278, 350
AsuNHI GCTAGC 1 cut(s) 670
AvaII GGWCC 1 cut(s) 218
BauI CACGAG 2 cut(s) 177, 699
BbvI GCAGC 4 cut(s) 15, 91, 107, 317
BccI CCATC 2 cut(s) 162, 194
BceAI ACGGC 1 cut(s) 67
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 2 cut(s) 378, 498
BfaI CTAG 3 cut(s) 108, 347, 671
BfmI CTRYAG 1 cut(s) 93
BfuAI ACCTGC 2 cut(s) 78, 335
BisI GCNGC 6 cut(s) 12, 29, 96, 105, 331, 447
BlsI GCNGC 6 cut(s) 13, 30, 97, 106, 332, 448
Bme1390I CCNGG 2 cut(s) 378, 498
Bme18I GGWCC 1 cut(s) 218
BmgT120I GGNCC 3 cut(s) 218, 278, 350
BmrFI CCNGG 2 cut(s) 378, 498
BmrI ACTGGG 1 cut(s) 134
BmsI GCATC 2 cut(s) 62, 419
BmtI GCTAGC 1 cut(s) 674
BmuI ACTGGG 1 cut(s) 134
BplI GAGNNNNNCTC 2 cut(s) 30, 32
Bpu10I CCTNAGC 1 cut(s) 70
BpuEI CTTGAG 3 cut(s) 43, 448, 470
BsaJI CCNNGG 1 cut(s) 551
Bsc4I CCNNNNNNNGG 1 cut(s) 227
Bse1I ACTGG 2 cut(s) 129, 461
BseBI CCWGG 2 cut(s) 378, 498
BseDI CCNNGG 1 cut(s) 551
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 1 cut(s) 227
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 2 cut(s) 129, 461
BseXI GCAGC 4 cut(s) 15, 91, 107, 317
BsgI GTGCAG 2 cut(s) 106, 423
BshFI GGCC 5 cut(s) 279, 351, 580, 598, 617
BsiSI CCGG 1 cut(s) 577
BslI CCNNNNNNNGG 1 cut(s) 227
BsnI GGCC 5 cut(s) 279, 351, 580, 598, 617
Bsp143I GATC 4 cut(s) 32, 147, 390, 646
BspACI CCGC 4 cut(s) 11, 67, 447, 686
BspANI GGCC 5 cut(s) 279, 351, 580, 598, 617
BspCNI CTCAG 1 cut(s) 83
BspMAI CTGCAG 1 cut(s) 97
BspMI ACCTGC 2 cut(s) 78, 335
BspOI GCTAGC 1 cut(s) 674
BspQI GCTCTTC 1 cut(s) 456
BsrBI CCGCTC 3 cut(s) 11, 69, 449
BsrI ACTGG 2 cut(s) 129, 461
BssECI CCNNGG 1 cut(s) 551
BssMI GATC 4 cut(s) 32, 147, 390, 646
BssSI CACGAG 2 cut(s) 177, 699
Bst2BI CACGAG 2 cut(s) 177, 699
Bst2UI CCWGG 2 cut(s) 378, 498
Bst4CI ACNGT 2 cut(s) 39, 509
Bst6I CTCTTC 1 cut(s) 456
BstAPI GCANNNNNTGC 1 cut(s) 104
BstC8I GCNNGC 3 cut(s) 537, 582, 672
BstDEI CTNAG 1 cut(s) 70
BstF5I GGATG 1 cut(s) 53
BstKTI GATC 4 cut(s) 35, 150, 393, 649
BstMBI GATC 4 cut(s) 32, 147, 390, 646
BstMWI GCNNNNNNNGC 6 cut(s) 101, 104, 179, 210, 231, 671
BstNI CCWGG 2 cut(s) 378, 498
BstSCI CCNGG 2 cut(s) 376, 496
BstSFI CTRYAG 1 cut(s) 93
BstV1I GCAGC 4 cut(s) 15, 91, 107, 317
BsuRI GGCC 5 cut(s) 279, 351, 580, 598, 617
BtsCI GGATG 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 3 cut(s) 411, 423, 454
BveI ACCTGC 2 cut(s) 78, 335
Cac8I GCNNGC 3 cut(s) 537, 582, 672
CaiI CAGNNNCTG 1 cut(s) 28
Cfr13I GGNCC 3 cut(s) 218, 278, 350
CseI GACGC 1 cut(s) 650
CviAII CATG 2 cut(s) 99, 531
DdeI CTNAG 1 cut(s) 70
DpnI GATC 4 cut(s) 34, 149, 392, 648
DpnII GATC 4 cut(s) 32, 147, 390, 646
EaeI YGGCCR 1 cut(s) 578
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco147I AGGCCT 1 cut(s) 598
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 218
Eco57I CTGAAG 2 cut(s) 5, 399
EcoO109I RGGNCCY 1 cut(s) 350
EcoRII CCWGG 2 cut(s) 376, 496
EcoRV GATATC 1 cut(s) 574
FaeI CATG 2 cut(s) 102, 534
FaiI YATR 8 cut(s) 100, 276, 368, 383, 472, 532, 657, 724
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FatI CATG 2 cut(s) 98, 530
FauI CCCGC 1 cut(s) 693
FblI GTMKAC 1 cut(s) 323
Fnu4HI GCNGC 6 cut(s) 12, 29, 96, 105, 331, 447
FokI GGATG 1 cut(s) 40
Fsp4HI GCNGC 6 cut(s) 12, 29, 96, 105, 331, 447
FspBI CTAG 3 cut(s) 108, 347, 671
GluI GCNGC 6 cut(s) 12, 29, 96, 105, 331, 447
HaeIII GGCC 5 cut(s) 279, 351, 580, 598, 617
HapII CCGG 1 cut(s) 577
HgaI GACGC 1 cut(s) 650
Hin1II CATG 2 cut(s) 102, 534
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 2 cut(s) 257, 704
HpaII CCGG 1 cut(s) 577
Hpy166II GTNNAC 3 cut(s) 271, 324, 640
Hpy188I TCNGA 4 cut(s) 114, 505, 552, 571
Hpy188III TCNNGA 7 cut(s) 42, 145, 298, 394, 427, 463, 701
Hpy8I GTNNAC 3 cut(s) 271, 324, 640
HpyAV CCTTC 2 cut(s) 29, 391
HpyCH4III ACNGT 2 cut(s) 39, 509
HpyCH4V TGCA 4 cut(s) 87, 95, 404, 539
HpyF10VI GCNNNNNNNGC 6 cut(s) 101, 104, 179, 210, 231, 671
HpyF3I CTNAG 1 cut(s) 70
Hsp92II CATG 2 cut(s) 102, 534
Kzo9I GATC 4 cut(s) 32, 147, 390, 646
LguI GCTCTTC 1 cut(s) 456
Lsp1109I GCAGC 4 cut(s) 15, 91, 107, 317
LweI GCATC 2 cut(s) 62, 419
MaeI CTAG 3 cut(s) 108, 347, 671
MaeIII GTNAC 2 cut(s) 463, 604
MalI GATC 4 cut(s) 34, 149, 392, 648
MbiI CCGCTC 3 cut(s) 11, 69, 449
MboI GATC 4 cut(s) 32, 147, 390, 646
MboII GAAGA 4 cut(s) 206, 257, 405, 443
MluCI AATT 2 cut(s) 237, 563
MlyI GAGTC 2 cut(s) 251, 713
MnlI CCTC 4 cut(s) 59, 67, 508, 546
MseI TTAA 3 cut(s) 561, 612, 714
MspI CCGG 1 cut(s) 577
MspR9I CCNGG 2 cut(s) 378, 498
MvaI CCWGG 2 cut(s) 378, 498
MwoI GCNNNNNNNGC 6 cut(s) 101, 104, 179, 210, 231, 671
NdeII GATC 4 cut(s) 32, 147, 390, 646
NheI GCTAGC 1 cut(s) 670
NlaIII CATG 2 cut(s) 102, 534
NmuCI GTSAC 2 cut(s) 463, 604
PceI AGGCCT 1 cut(s) 598
PciSI GCTCTTC 1 cut(s) 456
PkrI GCNGC 6 cut(s) 13, 30, 97, 106, 332, 448
PleI GAGTC 2 cut(s) 251, 712
PpsI GAGTC 2 cut(s) 251, 712
Psp6I CCWGG 2 cut(s) 376, 496
PspGI CCWGG 2 cut(s) 376, 496
PspPI GGNCC 3 cut(s) 218, 278, 350
PstI CTGCAG 1 cut(s) 97
PstNI CAGNNNCTG 1 cut(s) 28
SalI GTCGAC 1 cut(s) 322
SapI GCTCTTC 1 cut(s) 456
SaqAI TTAA 3 cut(s) 561, 612, 714
SatI GCNGC 6 cut(s) 12, 29, 96, 105, 331, 447
Sau3AI GATC 4 cut(s) 32, 147, 390, 646
Sau96I GGNCC 3 cut(s) 218, 278, 350
SchI GAGTC 2 cut(s) 251, 713
ScrFI CCNGG 2 cut(s) 378, 498
SetI ASST 8 cut(s) 51, 65, 76, 92, 329, 365, 486, 519
SfaNI GCATC 2 cut(s) 62, 419
SfcI CTRYAG 1 cut(s) 93
SinI GGWCC 1 cut(s) 218
SmlI CTYRAG 3 cut(s) 58, 427, 485
SmoI CTYRAG 3 cut(s) 58, 427, 485
Sse9I AATT 2 cut(s) 237, 563
SseBI AGGCCT 1 cut(s) 598
SsiI CCGC 4 cut(s) 11, 67, 447, 686
SspMI CTAG 3 cut(s) 108, 347, 671
StuI AGGCCT 1 cut(s) 598
StyD4I CCNGG 2 cut(s) 376, 496
TaaI ACNGT 2 cut(s) 39, 509
TaqI TCGA 2 cut(s) 144, 323
TasI AATT 2 cut(s) 237, 563
TauI GCSGC 2 cut(s) 14, 449
Tru1I TTAA 3 cut(s) 561, 612, 714
Tru9I TTAA 3 cut(s) 561, 612, 714
TscAI CASTG 3 cut(s) 411, 423, 461
TseFI GTSAC 2 cut(s) 463, 604
TseI GCWGC 4 cut(s) 28, 95, 104, 330
Tsp45I GTSAC 2 cut(s) 463, 604
TspDTI ATGAA 3 cut(s) 264, 398, 535
TspRI CASTG 3 cut(s) 411, 423, 461
VpaK11BI GGWCC 1 cut(s) 218
XapI RAATTY 1 cut(s) 563
XmiI GTMKAC 1 cut(s) 323
XspI CTAG 3 cut(s) 108, 347, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.