FvH4_7g21740

Ribosomal protein S12/S23

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005269
Physical Location & Seq
Forward (+)
17309910 .. 17312879
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g21740.t1

Sequence Viewer

Length: 885 bp
ATGTATCGAATCGCTTCAAACTTAGCTTCCTCCTTGGCCTCATCTACTTCCAAGAAGCTGGTCTGCGGTAGGGTTGTATGCAATAGGAGCTATGCGGCGCAAGATCTCACCTTGGAGATTGGGCCACGCGCCGCCTTGCTTGAGGCTGCGGAGGCTGCCAGGTTTACCATGGGCGCTCTGGTGCGACGTGTCTCTGAGCTTGGGGAGGCCTTCAGAGTTACGATGGGATCAAAGGATGGTGTTACTGTTAAGAATGTCAGTAAGACACTTGCGGAACAGTTCGCCGGAGCTGCTAATACTGCTGGAGGAGATGGTACAACCTCTGCAGCTGTCTTGACCCAGGCAACACTCACAGAAGGTTGCCACTCCATAGATGCTAGGGTTTCTTTCCTCAAAGATACCGGTATTGGGGAAAATAGGAGAGCCAGTTTAGATGATCTAGCCATCCTCACTGGGCGAGAGGTTATAAGTGAAGATAACAGTTTCATTATTGACAGAATGCTCATGTCACCCACATTAAATCAATTGATTCGTCATAATAGAACTGAGAAAAGTCGCACGAACCGTAATCGAGCTTTGGATAAATGCCCCCAAAAGCAAGGAGTATGCCTGCGAGTTTTTACAAGAACACCCAAGAAGCCAAATTCAGCTCTGCGTAAGGTAGCCAAAGTAAGGCTGAGCAATCGAAATGATGTATTTTCGTACATTCCAGGTGAAGGTCATAATTTGCAGGAACATTCTATGGTCTTAATAAGAGGGGGCAGAGTGAAAGATTTGCCTGGTGTGAAATTCCACTGTATCAGAGGAGTTAAGGATTTGCTTGGACTTCCAAACCGAAGAAGAGGCCGATCAAAATATGGTGCAGAAAAGCCAAAGAGCGCCTGA

Protein Analysis

295

Amino Acids

32.01

Weight (kDa)

10.26

Isoelectric Point (pI)

35.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 188 - 292 7.6e-37 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 467
AccII CGCG 1 cut(s) 129
AciI CCGC 5 cut(s) 66, 95, 132, 149, 272
AclWI GGATC 1 cut(s) 235
AcsI RAATTY 2 cut(s) 643, 788
AcuI CTGAAG 1 cut(s) 196
AfaI GTAC 2 cut(s) 316, 704
AfiI CCNNNNNNNGG 3 cut(s) 408, 672, 716
AflIII ACRYGT 1 cut(s) 187
AgeI ACCGGT 1 cut(s) 401
AgsI TTSAA 1 cut(s) 18
AjiI CACGTC 1 cut(s) 188
AjnI CCWGG 4 cut(s) 158, 339, 709, 778
AluBI AGCT 8 cut(s) 26, 58, 90, 199, 290, 329, 575, 650
AluI AGCT 8 cut(s) 26, 58, 90, 199, 290, 329, 575, 650
Alw26I GTCTC 1 cut(s) 196
AlwI GGATC 1 cut(s) 235
AoxI GGCC 4 cut(s) 36, 122, 207, 844
ApeKI GCWGC 4 cut(s) 146, 155, 290, 326
ApoI RAATTY 2 cut(s) 643, 788
AsiGI ACCGGT 1 cut(s) 401
Asp700I GAANNNNTTC 1 cut(s) 13
AspLEI GCGC 4 cut(s) 100, 131, 176, 881
AspS9I GGNCC 1 cut(s) 122
AsuHPI GGTGA 3 cut(s) 100, 501, 725
BbvI GCAGC 4 cut(s) 133, 142, 277, 338
BccI CCATC 4 cut(s) 217, 230, 305, 452
BciT130I CCWGG 4 cut(s) 160, 341, 711, 780
BcoDI GTCTC 1 cut(s) 196
BfaI CTAG 2 cut(s) 378, 440
BfmI CTRYAG 1 cut(s) 324
BfoI RGCGCY 2 cut(s) 177, 882
BglII AGATCT 1 cut(s) 103
BisI GCNGC 6 cut(s) 96, 132, 147, 156, 291, 327
BlpI GCTNAGC 1 cut(s) 677
BlsI GCNGC 6 cut(s) 97, 133, 148, 157, 292, 328
Bme1390I CCNGG 4 cut(s) 160, 341, 711, 780
BmgBI CACGTC 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 122
BmrFI CCNGG 4 cut(s) 160, 341, 711, 780
BmrI ACTGGG 1 cut(s) 462
BmsI GCATC 1 cut(s) 364
BmuI ACTGGG 1 cut(s) 462
BpmI CTGGAG 1 cut(s) 324
Bpu1102I GCTNAGC 1 cut(s) 677
BpuEI CTTGAG 1 cut(s) 161
BsaJI CCNNGG 4 cut(s) 33, 111, 168, 339
BsaWI WCCGGW 1 cut(s) 401
BsaXI ACNNNNNCTCC 2 cut(s) 412, 442
Bsc4I CCNNNNNNNGG 3 cut(s) 408, 672, 716
Bse118I RCCGGY 1 cut(s) 401
Bse1I ACTGG 2 cut(s) 426, 457
BseBI CCWGG 4 cut(s) 160, 341, 711, 780
BseDI CCNNGG 4 cut(s) 33, 111, 168, 339
BseGI GGATG 2 cut(s) 241, 444
BseLI CCNNNNNNNGG 3 cut(s) 408, 672, 716
BseMII CTCAG 3 cut(s) 186, 537, 668
BseNI ACTGG 2 cut(s) 426, 457
BseRI GAGGAG 2 cut(s) 321, 819
BseXI GCAGC 4 cut(s) 133, 142, 277, 338
BsgI GTGCAG 1 cut(s) 882
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 4 cut(s) 38, 124, 209, 846
BshTI ACCGGT 1 cut(s) 401
BsiSI CCGG 2 cut(s) 285, 402
BslI CCNNNNNNNGG 3 cut(s) 408, 672, 716
BsmAI GTCTC 1 cut(s) 196
BsmI GAATGC 1 cut(s) 504
BsnI GGCC 4 cut(s) 38, 124, 209, 846
Bsp143I GATC 4 cut(s) 103, 227, 436, 848
Bsp1720I GCTNAGC 1 cut(s) 677
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 5 cut(s) 66, 95, 132, 149, 272
BspANI GGCC 4 cut(s) 38, 124, 209, 846
BspCNI CTCAG 3 cut(s) 187, 538, 669
BspFNI CGCG 1 cut(s) 129
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 1 cut(s) 235
BsrFI RCCGGY 1 cut(s) 401
BsrI ACTGG 2 cut(s) 426, 457
BssAI RCCGGY 1 cut(s) 401
BssECI CCNNGG 4 cut(s) 33, 111, 168, 339
BssMI GATC 4 cut(s) 103, 227, 436, 848
BssT1I CCWWGG 3 cut(s) 33, 111, 168
Bst2UI CCWGG 4 cut(s) 160, 341, 711, 780
Bst4CI ACNGT 5 cut(s) 247, 279, 482, 566, 797
Bst6I CTCTTC 1 cut(s) 835
BstC8I GCNNGC 1 cut(s) 611
BstDEI CTNAG 4 cut(s) 22, 195, 546, 677
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 2 cut(s) 241, 444
BstFNI CGCG 1 cut(s) 129
BstH2I RGCGCY 2 cut(s) 177, 882
BstHHI GCGC 4 cut(s) 100, 131, 176, 881
BstKTI GATC 4 cut(s) 106, 230, 439, 851
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 4 cut(s) 103, 227, 436, 848
BstMWI GCNNNNNNNGC 5 cut(s) 87, 152, 155, 290, 299
BstNI CCWGG 4 cut(s) 160, 341, 711, 780
BstSCI CCNGG 4 cut(s) 158, 339, 709, 778
BstSFI CTRYAG 1 cut(s) 324
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 4 cut(s) 133, 142, 277, 338
BstX2I RGATCY 1 cut(s) 103
BstXI CCANNNNNNTGG 1 cut(s) 58
BstYI RGATCY 1 cut(s) 103
BsuRI GGCC 4 cut(s) 38, 124, 209, 846
BtgI CCRYGG 1 cut(s) 168
BtrI CACGTC 1 cut(s) 188
BtsCI GGATG 2 cut(s) 241, 444
BtsIMutI CAGTG 2 cut(s) 450, 793
Cac8I GCNNGC 1 cut(s) 611
CfoI GCGC 4 cut(s) 100, 131, 176, 881
Cfr10I RCCGGY 1 cut(s) 401
Cfr13I GGNCC 1 cut(s) 122
Csp6I GTAC 2 cut(s) 315, 703
CspAI ACCGGT 1 cut(s) 401
CviAII CATG 2 cut(s) 169, 505
CviQI GTAC 2 cut(s) 315, 703
DdeI CTNAG 4 cut(s) 22, 195, 546, 677
DpnI GATC 4 cut(s) 105, 229, 438, 850
DpnII GATC 4 cut(s) 103, 227, 436, 848
Eam1104I CTCTTC 1 cut(s) 835
EarI CTCTTC 1 cut(s) 835
Eco130I CCWWGG 3 cut(s) 33, 111, 168
Eco147I AGGCCT 1 cut(s) 209
Eco57I CTGAAG 1 cut(s) 196
EcoRII CCWGG 4 cut(s) 158, 339, 709, 778
EcoT14I CCWWGG 3 cut(s) 33, 111, 168
ErhI CCWWGG 3 cut(s) 33, 111, 168
FaeI CATG 2 cut(s) 172, 508
FatI CATG 2 cut(s) 168, 504
Fnu4HI GCNGC 6 cut(s) 96, 132, 147, 156, 291, 327
FokI GGATG 2 cut(s) 248, 431
Fsp4HI GCNGC 6 cut(s) 96, 132, 147, 156, 291, 327
FspBI CTAG 2 cut(s) 378, 440
GlaI GCGC 4 cut(s) 99, 130, 175, 880
GluI GCNGC 6 cut(s) 96, 132, 147, 156, 291, 327
GsuI CTGGAG 1 cut(s) 324
HaeII RGCGCY 2 cut(s) 177, 882
HaeIII GGCC 4 cut(s) 38, 124, 209, 846
HapII CCGG 2 cut(s) 285, 402
HhaI GCGC 4 cut(s) 100, 131, 176, 881
Hin1II CATG 2 cut(s) 172, 508
Hin6I GCGC 4 cut(s) 98, 129, 174, 879
HinP1I GCGC 4 cut(s) 98, 129, 174, 879
HinfI GANTC 2 cut(s) 9, 529
HpaII CCGG 2 cut(s) 285, 402
HphI GGTGA 3 cut(s) 100, 501, 725
Hpy166II GTNNAC 1 cut(s) 165
Hpy188I TCNGA 3 cut(s) 196, 215, 803
Hpy188III TCNNGA 1 cut(s) 334
Hpy8I GTNNAC 1 cut(s) 165
Hpy99I CGWCG 1 cut(s) 189
HpyAV CCTTC 3 cut(s) 220, 350, 710
HpyCH4III ACNGT 5 cut(s) 247, 279, 482, 566, 797
HpyCH4IV ACGT 1 cut(s) 187
HpyCH4V TGCA 4 cut(s) 81, 326, 730, 863
HpyF10VI GCNNNNNNNGC 5 cut(s) 87, 152, 155, 290, 299
HpyF3I CTNAG 4 cut(s) 22, 195, 546, 677
HpySE526I ACGT 1 cut(s) 187
Hsp92II CATG 2 cut(s) 172, 508
HspAI GCGC 4 cut(s) 98, 129, 174, 879
Kzo9I GATC 4 cut(s) 103, 227, 436, 848
LmnI GCTCC 2 cut(s) 87, 287
Lsp1109I GCAGC 4 cut(s) 133, 142, 277, 338
LweI GCATC 1 cut(s) 364
MaeI CTAG 2 cut(s) 378, 440
MaeII ACGT 1 cut(s) 187
MaeIII GTNAC 3 cut(s) 217, 241, 507
MalI GATC 4 cut(s) 105, 229, 438, 850
MboI GATC 4 cut(s) 103, 227, 436, 848
MboII GAAGA 3 cut(s) 485, 849, 852
MfeI CAATTG 1 cut(s) 524
MflI RGATCY 1 cut(s) 103
MluCI AATT 4 cut(s) 524, 643, 724, 788
MroXI GAANNNNTTC 1 cut(s) 13
MseI TTAA 4 cut(s) 249, 518, 749, 810
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 2 cut(s) 285, 402
MspR9I CCNGG 4 cut(s) 160, 341, 711, 780
MunI CAATTG 1 cut(s) 524
Mva1269I GAATGC 1 cut(s) 504
MvaI CCWGG 4 cut(s) 160, 341, 711, 780
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 5 cut(s) 87, 152, 155, 290, 299
NcoI CCATGG 1 cut(s) 168
NdeII GATC 4 cut(s) 103, 227, 436, 848
NlaIII CATG 2 cut(s) 172, 508
NmuCI GTSAC 1 cut(s) 507
PceI AGGCCT 1 cut(s) 209
PcsI WCGNNNNNNNCGW 1 cut(s) 562
PctI GAATGC 1 cut(s) 504
PdmI GAANNNNTTC 1 cut(s) 13
PfeI GAWTC 2 cut(s) 9, 529
PinAI ACCGGT 1 cut(s) 401
PkrI GCNGC 6 cut(s) 97, 133, 148, 157, 292, 328
PsiI TTATAA 1 cut(s) 467
Psp6I CCWGG 4 cut(s) 158, 339, 709, 778
PspGI CCWGG 4 cut(s) 158, 339, 709, 778
PspPI GGNCC 1 cut(s) 122
PstI CTGCAG 1 cut(s) 328
PsuI RGATCY 1 cut(s) 103
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 2 cut(s) 316, 704
RsaNI GTAC 2 cut(s) 315, 703
SaqAI TTAA 4 cut(s) 249, 518, 749, 810
SatI GCNGC 6 cut(s) 96, 132, 147, 156, 291, 327
Sau3AI GATC 4 cut(s) 103, 227, 436, 848
Sau96I GGNCC 1 cut(s) 122
ScrFI CCNGG 4 cut(s) 160, 341, 711, 780
SfaNI GCATC 1 cut(s) 364
SfcI CTRYAG 1 cut(s) 324
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
Sse9I AATT 4 cut(s) 524, 643, 724, 788
SseBI AGGCCT 1 cut(s) 209
SsiI CCGC 5 cut(s) 66, 95, 132, 149, 272
SspMI CTAG 2 cut(s) 378, 440
StuI AGGCCT 1 cut(s) 209
StyD4I CCNGG 4 cut(s) 158, 339, 709, 778
StyI CCWWGG 3 cut(s) 33, 111, 168
TaaI ACNGT 5 cut(s) 247, 279, 482, 566, 797
TaiI ACGT 1 cut(s) 190
TaqI TCGA 3 cut(s) 7, 571, 685
TasI AATT 4 cut(s) 524, 643, 724, 788
TauI GCSGC 2 cut(s) 98, 134
TfiI GAWTC 2 cut(s) 9, 529
Tru1I TTAA 4 cut(s) 249, 518, 749, 810
Tru9I TTAA 4 cut(s) 249, 518, 749, 810
TscAI CASTG 2 cut(s) 457, 800
TseFI GTSAC 1 cut(s) 507
TseI GCWGC 4 cut(s) 146, 155, 290, 326
Tsp45I GTSAC 1 cut(s) 507
TspDTI ATGAA 1 cut(s) 475
TspRI CASTG 2 cut(s) 457, 800
XapI RAATTY 2 cut(s) 643, 788
XcmI CCANNNNNNNNNTGG 2 cut(s) 166, 175
XmnI GAANNNNTTC 1 cut(s) 13
XspI CTAG 2 cut(s) 378, 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.