FvH4_7g22770

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0011170
Physical Location & Seq
Forward (+)
17895061 .. 17897596
2536 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22770.t1

Sequence Viewer

Length: 708 bp
ATGAGTGGGGTAGGAAAGCCTGACTTTTTCTACAGAGAAGCTCAGCGCCTGGGTTATGTAGCTCGCTCCGCCTTCAAGCTGCTTCAGATTCAAAAGCAGTACAAGCTCATAACACCAGGCTCCTCTGTTCTTGACCTAGGTTGTGCCCCTGGTGCTTGGCTTCAGGTGGCTTGTCAGAGCTTGGGTCCTATCAAAAATGGTGGCCTTGTTGTTGGGATTGATCTCAAGAAGGTGAAGGTTCCAGCTATGCATTGTGATTCAAGGGTTCAAACTGTTTGTGCTGATGTTATGAAGCTCCCTAAAACCTCAGTCAGGGAACTTTCTCCACAGAAAGGGTTTTCTGTGATACTCTCAGATATGTGTCCCCTCGTTTCTGGAATAGCAAGTAAAGATGCAGCTTTATCCCTCGAGTTAGGCATGCAAGCACTGGATTTGGCTGTTGGTGGAGCAGCATTAGCTAATTCCAACGATGAAACTGAAGAAGAAAATGTCGGTGAATCTAATACAAGTTCAGATGTTAATGGTGTATTGAGAAGGGGCGGTCATCTTGTCATAAAGCTTTTAGAGAGTGAAGATGTGCAAGATTTTAACCGCATCTGCAAACCACTGTTTAGAAAGGCATCATTGCTGAGGCCTAAAGCTACACGATCGACATCAAGAGAGATTTATTTGATATGTCAAGGTCTGCAGTCACAAGCTAGAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

236

Amino Acids

25.32

Weight (kDa)

8.96

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 19 - 228 7.6e-40 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13830
fragaria_vesca FvH4_7g22770
malus_domestica MD07G1200600.v1.1
prunus_persica Prupe.2G237400_v2.0.a1
pyrus_communis pycom01g15670 pycom07g18760 pycom07g18770
rosa_chinensis RchiOBHm_Chr1g0367871
rosa_laevigata RLG00000027260
rosa_multiflora Rmu_co8050104.1_g000001 Rmu_sc0010008.1_g000001 Rmu_ssc0000395.1_g000039
rosa_roxburghii Rroxscaffold_4G00289170
rosa_rugosa Rorug01G0337300 Rorug01G0337400
rosa_samantha Rh1AG344200 Rh1BG306200 Rh1CG321800 Rh1DG338400
rosa_wichuraiana Rw1G030640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 69, 540, 592
AcuI CTGAAG 3 cut(s) 68, 146, 498
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 2 cut(s) 312, 332
AgsI TTSAA 4 cut(s) 76, 92, 261, 269
AjnI CCWGG 3 cut(s) 48, 115, 148
AlwNI CAGNNNCTG 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 407
AoxI GGCC 2 cut(s) 202, 632
ApeKI GCWGC 3 cut(s) 79, 395, 449
AspA2I CCTAGG 1 cut(s) 136
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 244, 506
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 1 cut(s) 185
AvrII CCTAGG 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 148
BbvCI CCTCAGC 1 cut(s) 629
BbvI GCAGC 3 cut(s) 66, 407, 461
BciT130I CCWGG 3 cut(s) 50, 117, 150
BfaI CTAG 2 cut(s) 137, 699
BfmI CTRYAG 2 cut(s) 31, 686
BfoI RGCGCY 1 cut(s) 49
BisI GCNGC 3 cut(s) 80, 396, 450
BlnI CCTAGG 1 cut(s) 136
BlpI GCTNAGC 1 cut(s) 42
BlsI GCNGC 3 cut(s) 81, 397, 451
Bme1390I CCNGG 3 cut(s) 50, 117, 150
Bme18I GGWCC 1 cut(s) 185
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 3 cut(s) 121, 186, 240
BmrFI CCNGG 3 cut(s) 50, 117, 150
BmsI GCATC 3 cut(s) 382, 603, 629
Bpu10I CCTNAGC 1 cut(s) 629
Bpu1102I GCTNAGC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 209
BsaBI GATNNNNATC 1 cut(s) 652
BsaJI CCNNGG 3 cut(s) 49, 136, 148
Bsc4I CCNNNNNNNGG 2 cut(s) 312, 332
Bse1I ACTGG 1 cut(s) 432
Bse3DI GCAATG 1 cut(s) 623
Bse8I GATNNNNATC 1 cut(s) 652
BseBI CCWGG 3 cut(s) 50, 117, 150
BseDI CCNNGG 3 cut(s) 49, 136, 148
BseJI GATNNNNATC 1 cut(s) 652
BseLI CCNNNNNNNGG 2 cut(s) 312, 332
BseMI GCAATG 1 cut(s) 623
BseMII CTCAG 4 cut(s) 56, 321, 366, 620
BseNI ACTGG 1 cut(s) 432
BseRI GAGGAG 1 cut(s) 112
BseSI GKGCMC 1 cut(s) 148
BseXI GCAGC 3 cut(s) 66, 407, 461
Bsh1285I CGRYCG 1 cut(s) 650
BshFI GGCC 2 cut(s) 204, 634
BsiEI CGRYCG 1 cut(s) 650
BsiHKCI CYCGRG 1 cut(s) 407
BslFI GGGAC 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 312, 332
BsmFI GGGAC 1 cut(s) 348
BsnI GGCC 2 cut(s) 204, 634
BsoBI CYCGRG 1 cut(s) 407
Bsp1286I GDGCHC 1 cut(s) 148
Bsp143I GATC 2 cut(s) 220, 647
Bsp1720I GCTNAGC 1 cut(s) 42
BspACI CCGC 3 cut(s) 69, 540, 592
BspANI GGCC 2 cut(s) 204, 634
BspCNI CTCAG 4 cut(s) 55, 320, 365, 621
BspLI GGNNCC 3 cut(s) 121, 186, 240
BspMAI CTGCAG 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 623
BsrI ACTGG 1 cut(s) 432
BssECI CCNNGG 3 cut(s) 49, 136, 148
BssMI GATC 2 cut(s) 220, 647
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 3 cut(s) 50, 117, 150
Bst4CI ACNGT 2 cut(s) 274, 609
BstC8I GCNNGC 3 cut(s) 64, 419, 423
BstDEI CTNAG 4 cut(s) 42, 307, 352, 629
BstENI CCTNNNNNAGG 1 cut(s) 310
BstH2I RGCGCY 1 cut(s) 49
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 2 cut(s) 223, 650
BstMBI GATC 2 cut(s) 220, 647
BstMCI CGRYCG 1 cut(s) 650
BstMWI GCNNNNNNNGC 4 cut(s) 68, 103, 152, 455
BstNI CCWGG 3 cut(s) 50, 117, 150
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 3 cut(s) 48, 115, 148
BstSFI CTRYAG 2 cut(s) 31, 686
BstSLI GKGCMC 1 cut(s) 148
BstV1I GCAGC 3 cut(s) 66, 407, 461
BsuRI GGCC 2 cut(s) 204, 634
BtsIMutI CAGTG 2 cut(s) 425, 605
Cac8I GCNNGC 3 cut(s) 64, 419, 423
CaiI CAGNNNCTG 1 cut(s) 49
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 100
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 1 cut(s) 418
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 4 cut(s) 42, 307, 352, 629
DpnI GATC 2 cut(s) 222, 649
DpnII GATC 2 cut(s) 220, 647
EciI GGCGGA 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 634
Eco47I GGWCC 1 cut(s) 185
Eco57I CTGAAG 3 cut(s) 68, 146, 498
Eco88I CYCGRG 1 cut(s) 407
EcoNI CCTNNNNNAGG 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 185
EcoRII CCWGG 3 cut(s) 48, 115, 148
EcoT14I CCWWGG 1 cut(s) 136
EcoT22I ATGCAT 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 1 cut(s) 421
FaiI YATR 9 cut(s) 57, 110, 248, 290, 359, 419, 554, 676, 706
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FaqI GGGAC 1 cut(s) 348
FatI CATG 1 cut(s) 417
Fnu4HI GCNGC 3 cut(s) 80, 396, 450
Fsp4HI GCNGC 3 cut(s) 80, 396, 450
FspBI CTAG 2 cut(s) 137, 699
GlaI GCGC 1 cut(s) 47
GluI GCNGC 3 cut(s) 80, 396, 450
HaeII RGCGCY 1 cut(s) 49
HaeIII GGCC 2 cut(s) 204, 634
HhaI GCGC 1 cut(s) 48
Hin1II CATG 1 cut(s) 421
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 3 cut(s) 88, 257, 497
HphI GGTGA 2 cut(s) 244, 506
Hpy188I TCNGA 4 cut(s) 87, 177, 355, 514
Hpy188III TCNNGA 4 cut(s) 131, 226, 375, 657
HpyAV CCTTC 4 cut(s) 82, 223, 229, 528
HpyCH4III ACNGT 2 cut(s) 274, 609
HpyCH4V TGCA 6 cut(s) 250, 395, 421, 580, 600, 688
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 103, 152, 455
HpyF3I CTNAG 4 cut(s) 42, 307, 352, 629
Hsp92II CATG 1 cut(s) 421
HspAI GCGC 1 cut(s) 46
Kzo9I GATC 2 cut(s) 220, 647
LmnI GCTCC 4 cut(s) 71, 125, 300, 446
Lsp1109I GCAGC 3 cut(s) 66, 407, 461
LweI GCATC 3 cut(s) 382, 603, 629
MaeI CTAG 2 cut(s) 137, 699
MaeIII GTNAC 1 cut(s) 690
MalI GATC 2 cut(s) 222, 649
MboI GATC 2 cut(s) 220, 647
MboII GAAGA 3 cut(s) 491, 494, 584
MhlI GDGCHC 1 cut(s) 148
MluCI AATT 1 cut(s) 460
MmeI TCCRAC 1 cut(s) 489
MnlI CCTC 5 cut(s) 133, 316, 377, 416, 624
Mph1103I ATGCAT 1 cut(s) 252
MseI TTAA 2 cut(s) 519, 588
MspR9I CCNGG 3 cut(s) 50, 117, 150
MvaI CCWGG 3 cut(s) 50, 117, 150
MwoI GCNNNNNNNGC 4 cut(s) 68, 103, 152, 455
NdeII GATC 2 cut(s) 220, 647
NlaIII CATG 1 cut(s) 421
NlaIV GGNNCC 3 cut(s) 121, 186, 240
NmuCI GTSAC 1 cut(s) 690
NsiI ATGCAT 1 cut(s) 252
NspI RCATGY 1 cut(s) 421
PaeI GCATGC 1 cut(s) 421
PaeR7I CTCGAG 1 cut(s) 407
PceI AGGCCT 1 cut(s) 634
PfeI GAWTC 3 cut(s) 88, 257, 497
PkrI GCNGC 3 cut(s) 81, 397, 451
Ple19I CGATCG 1 cut(s) 650
PpuMI RGGWCCY 1 cut(s) 185
Psp5II RGGWCCY 1 cut(s) 185
Psp6I CCWGG 3 cut(s) 48, 115, 148
PspGI CCWGG 3 cut(s) 48, 115, 148
PspN4I GGNNCC 3 cut(s) 121, 186, 240
PspPI GGNCC 1 cut(s) 185
PspPPI RGGWCCY 1 cut(s) 185
PspXI VCTCGAGB 1 cut(s) 407
PstI CTGCAG 1 cut(s) 690
PstNI CAGNNNCTG 1 cut(s) 49
PvuI CGATCG 1 cut(s) 650
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SaqAI TTAA 2 cut(s) 519, 588
SatI GCNGC 3 cut(s) 80, 396, 450
Sau3AI GATC 2 cut(s) 220, 647
Sau96I GGNCC 1 cut(s) 185
ScrFI CCNGG 3 cut(s) 50, 117, 150
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 3 cut(s) 382, 603, 629
SfcI CTRYAG 2 cut(s) 31, 686
Sfr274I CTCGAG 1 cut(s) 407
SinI GGWCC 1 cut(s) 185
SlaI CTCGAG 1 cut(s) 407
SmlI CTYRAG 2 cut(s) 224, 407
SmoI CTYRAG 2 cut(s) 224, 407
SphI GCATGC 1 cut(s) 421
Sse9I AATT 1 cut(s) 460
SseBI AGGCCT 1 cut(s) 634
SsiI CCGC 3 cut(s) 69, 540, 592
SspMI CTAG 2 cut(s) 137, 699
StuI AGGCCT 1 cut(s) 634
StyD4I CCNGG 3 cut(s) 48, 115, 148
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 2 cut(s) 274, 609
TaqI TCGA 2 cut(s) 408, 650
TasI AATT 1 cut(s) 460
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 3 cut(s) 88, 257, 497
Tru1I TTAA 2 cut(s) 519, 588
Tru9I TTAA 2 cut(s) 519, 588
TscAI CASTG 2 cut(s) 432, 612
TseFI GTSAC 1 cut(s) 690
TseI GCWGC 3 cut(s) 79, 395, 449
Tsp45I GTSAC 1 cut(s) 690
TspDTI ATGAA 2 cut(s) 305, 486
TspRI CASTG 2 cut(s) 432, 612
VpaK11BI GGWCC 1 cut(s) 185
XagI CCTNNNNNAGG 1 cut(s) 310
XceI RCATGY 1 cut(s) 421
XhoI CTCGAG 1 cut(s) 407
XmaJI CCTAGG 1 cut(s) 136
XspI CTAG 2 cut(s) 137, 699
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.