Rmu_ssc0000395.1_g000039

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000395.1
OG0011170
Physical Location & Seq
Forward (+)
169672 .. 171165
1494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000395.1_g000039.1.cds

Sequence Viewer

Length: 714 bp
atgagtggggcaggaaagccggactttttctacagagaagctcagcgcctgggttatgtagctcgctccgccttcaaggtactgcttcagatacagaagcagtacaagctcataacaccaggctcctctgttctggaccttggttgtgctcctggtgcttggcttcaggtggcttgtcagagcttgggtcctatcaaaaatggtggtcttgttgttgggattgatctcaagaaggtgaaggttccagctatgcattgtgattcaagggttcaaactctgtgtgctgatgttatgaagctccctaaaacctcagtcagggaactttctccacagaaggggttttctgtaatactctcagatatgtgtcccctcgtttctgggatagcaagtaaagatgcagctttatccctcgagttaggcatgcaagcactggatttggctgtaggtgaagcagcattagctaatcccaacaatgtaactcaagtggaagaaagtcttgttggagcaactacaagttcagatgttaatggtgtattgaaaaggggcggtcatcttgttataaagcttttagagagtgaagatgtgcaagatttcaaccgcatttgcaaaccactgtttagaaaggcatcattgttgaggcctaaagctacaagatcatcatcaagagagatttatttgatatgtcaaggtctgcagtcacaagcaagaacatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

237

Amino Acids

25.44

Weight (kDa)

9.28

Isoelectric Point (pI)

40.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13830
fragaria_vesca FvH4_7g22770
malus_domestica MD07G1200600.v1.1
prunus_persica Prupe.2G237400_v2.0.a1
pyrus_communis pycom01g15670 pycom07g18760 pycom07g18770
rosa_chinensis RchiOBHm_Chr1g0367871
rosa_laevigata RLG00000027260
rosa_multiflora Rmu_co8050104.1_g000001 Rmu_sc0010008.1_g000001 Rmu_ssc0000395.1_g000039
rosa_roxburghii Rroxscaffold_4G00289170
rosa_rugosa Rorug01G0337300 Rorug01G0337400
rosa_samantha Rh1AG344200 Rh1BG306200 Rh1CG321800 Rh1DG338400
rosa_wichuraiana Rw1G030640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AciI CCGC 3 cut(s) 69, 546, 598
AcuI CTGAAG 2 cut(s) 71, 149
AfaI GTAC 2 cut(s) 81, 104
AfiI CCNNNNNNNGG 2 cut(s) 315, 335
AgsI TTSAA 5 cut(s) 76, 264, 272, 538, 595
AjnI CCWGG 3 cut(s) 48, 118, 151
AjuI GAANNNNNNNTTGG 2 cut(s) 483, 515
Alw21I GWGCWC 1 cut(s) 151
AlwNI CAGNNNCTG 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 410
AoxI GGCC 1 cut(s) 638
ApeKI GCWGC 2 cut(s) 398, 452
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 2 cut(s) 136, 188
AsuHPI GGTGA 2 cut(s) 247, 458
AvaI CYCGRG 1 cut(s) 410
AvaII GGWCC 2 cut(s) 136, 188
Bbv12I GWGCWC 1 cut(s) 151
BbvI GCAGC 2 cut(s) 410, 464
BciT130I CCWGG 3 cut(s) 50, 120, 153
BfmI CTRYAG 3 cut(s) 31, 441, 692
BfoI RGCGCY 1 cut(s) 49
BisI GCNGC 2 cut(s) 399, 453
BlpI GCTNAGC 1 cut(s) 42
BlsI GCNGC 2 cut(s) 400, 454
Bme1390I CCNGG 3 cut(s) 50, 120, 153
Bme18I GGWCC 2 cut(s) 136, 188
BmeT110I CYCGRG 1 cut(s) 410
BmgT120I GGNCC 2 cut(s) 136, 188
BmiI GGNNCC 3 cut(s) 124, 189, 243
BmrFI CCNGG 3 cut(s) 50, 120, 153
BmsI GCATC 2 cut(s) 385, 635
Bpu1102I GCTNAGC 1 cut(s) 42
BpuEI CTTGAG 2 cut(s) 212, 465
BsaBI GATNNNNATC 1 cut(s) 658
BsaJI CCNNGG 2 cut(s) 49, 139
Bsc4I CCNNNNNNNGG 2 cut(s) 315, 335
Bse1I ACTGG 1 cut(s) 435
Bse8I GATNNNNATC 1 cut(s) 658
BseBI CCWGG 3 cut(s) 50, 120, 153
BseDI CCNNGG 2 cut(s) 49, 139
BseJI GATNNNNATC 1 cut(s) 658
BseLI CCNNNNNNNGG 2 cut(s) 315, 335
BseMII CTCAG 3 cut(s) 56, 324, 369
BseNI ACTGG 1 cut(s) 435
BseRI GAGGAG 1 cut(s) 115
BseXI GCAGC 2 cut(s) 410, 464
BshFI GGCC 1 cut(s) 640
BsiHKAI GWGCWC 1 cut(s) 151
BsiHKCI CYCGRG 1 cut(s) 410
BsiSI CCGG 1 cut(s) 20
BslFI GGGAC 1 cut(s) 351
BslI CCNNNNNNNGG 2 cut(s) 315, 335
BsmFI GGGAC 1 cut(s) 351
BsnI GGCC 1 cut(s) 640
BsoBI CYCGRG 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 151
Bsp143I GATC 2 cut(s) 223, 653
Bsp1720I GCTNAGC 1 cut(s) 42
BspACI CCGC 3 cut(s) 69, 546, 598
BspANI GGCC 1 cut(s) 640
BspCNI CTCAG 3 cut(s) 55, 323, 368
BspLI GGNNCC 3 cut(s) 124, 189, 243
BspMAI CTGCAG 1 cut(s) 696
BsrI ACTGG 1 cut(s) 435
BssECI CCNNGG 2 cut(s) 49, 139
BssMI GATC 2 cut(s) 223, 653
BssT1I CCWWGG 1 cut(s) 139
Bst2UI CCWGG 3 cut(s) 50, 120, 153
Bst4CI ACNGT 1 cut(s) 615
BstC8I GCNNGC 3 cut(s) 64, 422, 426
BstDEI CTNAG 3 cut(s) 42, 310, 355
BstENI CCTNNNNNAGG 1 cut(s) 313
BstH2I RGCGCY 1 cut(s) 49
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 2 cut(s) 226, 656
BstMBI GATC 2 cut(s) 223, 653
BstMWI GCNNNNNNNGC 4 cut(s) 68, 106, 155, 458
BstNI CCWGG 3 cut(s) 50, 120, 153
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 3 cut(s) 48, 118, 151
BstSFI CTRYAG 3 cut(s) 31, 441, 692
BstV1I GCAGC 2 cut(s) 410, 464
BsuRI GGCC 1 cut(s) 640
BtsIMutI CAGTG 2 cut(s) 428, 611
Cac8I GCNNGC 3 cut(s) 64, 422, 426
CaiI CAGNNNCTG 1 cut(s) 49
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 2 cut(s) 136, 188
Csp6I GTAC 2 cut(s) 80, 103
CspCI CAANNNNNGTGG 2 cut(s) 184, 219
CviAII CATG 1 cut(s) 421
CviQI GTAC 2 cut(s) 80, 103
DdeI CTNAG 3 cut(s) 42, 310, 355
DpnI GATC 2 cut(s) 225, 655
DpnII GATC 2 cut(s) 223, 653
EciI GGCGGA 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 139
Eco147I AGGCCT 1 cut(s) 640
Eco47I GGWCC 2 cut(s) 136, 188
Eco57I CTGAAG 2 cut(s) 71, 149
Eco88I CYCGRG 1 cut(s) 410
EcoNI CCTNNNNNAGG 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 188
EcoRII CCWGG 3 cut(s) 48, 118, 151
EcoT14I CCWWGG 1 cut(s) 139
EcoT22I ATGCAT 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 1 cut(s) 424
FaiI YATR 9 cut(s) 57, 113, 251, 293, 362, 422, 560, 682, 712
FalI AAGNNNNNCTT 4 cut(s) 8, 40, 480, 512
FaqI GGGAC 1 cut(s) 351
FatI CATG 1 cut(s) 420
Fnu4HI GCNGC 2 cut(s) 399, 453
Fsp4HI GCNGC 2 cut(s) 399, 453
GlaI GCGC 1 cut(s) 47
GluI GCNGC 2 cut(s) 399, 453
HaeII RGCGCY 1 cut(s) 49
HaeIII GGCC 1 cut(s) 640
HapII CCGG 1 cut(s) 20
HhaI GCGC 1 cut(s) 48
Hin1II CATG 1 cut(s) 424
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HindIII AAGCTT 1 cut(s) 563
HinfI GANTC 1 cut(s) 260
HpaII CCGG 1 cut(s) 20
HphI GGTGA 2 cut(s) 247, 458
Hpy188I TCNGA 4 cut(s) 90, 180, 358, 520
Hpy188III TCNNGA 3 cut(s) 134, 229, 663
HpyAV CCTTC 4 cut(s) 82, 226, 232, 328
HpyCH4III ACNGT 1 cut(s) 615
HpyCH4V TGCA 6 cut(s) 253, 398, 424, 586, 606, 694
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 106, 155, 458
HpyF3I CTNAG 3 cut(s) 42, 310, 355
Hsp92II CATG 1 cut(s) 424
HspAI GCGC 1 cut(s) 46
Kzo9I GATC 2 cut(s) 223, 653
LmnI GCTCC 5 cut(s) 71, 128, 154, 303, 503
Lsp1109I GCAGC 2 cut(s) 410, 464
LweI GCATC 2 cut(s) 385, 635
MaeIII GTNAC 2 cut(s) 475, 696
MalI GATC 2 cut(s) 225, 655
MboI GATC 2 cut(s) 223, 653
MboII GAAGA 2 cut(s) 500, 590
MhlI GDGCHC 1 cut(s) 151
MmeI TCCRAC 1 cut(s) 481
MnlI CCTC 5 cut(s) 136, 319, 380, 419, 630
Mph1103I ATGCAT 1 cut(s) 255
MseI TTAA 1 cut(s) 525
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 3 cut(s) 50, 120, 153
MvaI CCWGG 3 cut(s) 50, 120, 153
MwoI GCNNNNNNNGC 4 cut(s) 68, 106, 155, 458
NdeII GATC 2 cut(s) 223, 653
NlaIII CATG 1 cut(s) 424
NlaIV GGNNCC 3 cut(s) 124, 189, 243
NmuCI GTSAC 1 cut(s) 696
NsiI ATGCAT 1 cut(s) 255
NspI RCATGY 1 cut(s) 424
PaeI GCATGC 1 cut(s) 424
PaeR7I CTCGAG 1 cut(s) 410
PceI AGGCCT 1 cut(s) 640
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 2 cut(s) 400, 454
PpuMI RGGWCCY 1 cut(s) 188
PsiI TTATAA 1 cut(s) 560
Psp5II RGGWCCY 1 cut(s) 188
Psp6I CCWGG 3 cut(s) 48, 118, 151
PspGI CCWGG 3 cut(s) 48, 118, 151
PspN4I GGNNCC 3 cut(s) 124, 189, 243
PspPI GGNCC 2 cut(s) 136, 188
PspPPI RGGWCCY 1 cut(s) 188
PspXI VCTCGAGB 1 cut(s) 410
PstI CTGCAG 1 cut(s) 696
PstNI CAGNNNCTG 1 cut(s) 49
RsaI GTAC 2 cut(s) 81, 104
RsaNI GTAC 2 cut(s) 80, 103
SaqAI TTAA 1 cut(s) 525
SatI GCNGC 2 cut(s) 399, 453
Sau3AI GATC 2 cut(s) 223, 653
Sau96I GGNCC 2 cut(s) 136, 188
ScrFI CCNGG 3 cut(s) 50, 120, 153
SduI GDGCHC 1 cut(s) 151
SfaNI GCATC 2 cut(s) 385, 635
SfcI CTRYAG 3 cut(s) 31, 441, 692
Sfr274I CTCGAG 1 cut(s) 410
SinI GGWCC 2 cut(s) 136, 188
SlaI CTCGAG 1 cut(s) 410
SmlI CTYRAG 3 cut(s) 227, 410, 480
SmoI CTYRAG 3 cut(s) 227, 410, 480
SphI GCATGC 1 cut(s) 424
SseBI AGGCCT 1 cut(s) 640
SsiI CCGC 3 cut(s) 69, 546, 598
StuI AGGCCT 1 cut(s) 640
StyD4I CCNGG 3 cut(s) 48, 118, 151
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 1 cut(s) 615
TaqI TCGA 1 cut(s) 411
TatI WGTACW 1 cut(s) 102
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 1 cut(s) 525
Tru9I TTAA 1 cut(s) 525
TscAI CASTG 2 cut(s) 435, 618
TseFI GTSAC 1 cut(s) 696
TseI GCWGC 2 cut(s) 398, 452
Tsp45I GTSAC 1 cut(s) 696
TspDTI ATGAA 1 cut(s) 308
TspRI CASTG 2 cut(s) 435, 618
VpaK11BI GGWCC 2 cut(s) 136, 188
XagI CCTNNNNNAGG 1 cut(s) 313
XceI RCATGY 1 cut(s) 424
XhoI CTCGAG 1 cut(s) 410
Zsp2I ATGCAT 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.