Rroxscaffold_4G00289170

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0011170
Physical Location & Seq
Reverse (-)
9853178 .. 9855936
2759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00289170.1

Sequence Viewer

Length: 756 bp
ATGAGTGGGGCAGGAAAGCCGGACTTTTTCTACAGAGAAGCTCAGCGCCTGGGTTATGTAGCTCGCTCCGCCTTCAAGCTGCTTCAGATACAGAAGCAGTACAAGCTCATAACACCAGGCTCCTCTGTTCTGGACCTTGGTTGTGCTCCTGGTGCTTGGCTTCAGGTGGCTTGTCAGAGCTTGGGTCCTATCAAAAATGGTGGTCTTGTTGTTGGGATTGATCTCAAGAAGGTGAAGGTTCCAGCTATGCATTGTGATTCAAGGGTTCAAACTGTGTGTGCTGATGTTATGAAGCTCCCTAAAACCTCAGTCAGGGAACTTTCTCCACAGGGACTTGGTATTCCAAGAATTGTGTTAACCCTTTTCGATAACTTGAAGGGGTTTTCTGTAATACTCTCAGATATGTGTCCCCTCGTTTCTGGGATAGCAAGTAAAGATGCGGCTTTATCCCTCGAGTTAGGCATGCAAGCACTGGATTTGGCTGTAGGTGAAGCAGCATTAGCTAATCCCAACAATGTAACTCAAGTGGAAGAAAGTCTTGTTGGAGCAACTACAAGTTCAGATGTTAATGGTGTATTGAAAAGGGGCGGTCATCTTGTCATAAAGCTTTTAGAGAGTGAAGATGTGCAAGATTTCAACCGCATTTGCAAACCACTGTTTAGAAAGGCATCATTGTTGAGGCCTAAAGCTACAAGATCATCATCAAGAGAGATTTATTTGATATGTCAAGGTCTGCAGTCACAAGCAAGACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

251

Amino Acids

26.95

Weight (kDa)

9.28

Isoelectric Point (pI)

38.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 19 - 244 1.3e-38 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13830
fragaria_vesca FvH4_7g22770
malus_domestica MD07G1200600.v1.1
prunus_persica Prupe.2G237400_v2.0.a1
pyrus_communis pycom01g15670 pycom07g18760 pycom07g18770
rosa_chinensis RchiOBHm_Chr1g0367871
rosa_laevigata RLG00000027260
rosa_multiflora Rmu_co8050104.1_g000001 Rmu_sc0010008.1_g000001 Rmu_ssc0000395.1_g000039
rosa_roxburghii Rroxscaffold_4G00289170
rosa_rugosa Rorug01G0337300 Rorug01G0337400
rosa_samantha Rh1AG344200 Rh1BG306200 Rh1CG321800 Rh1DG338400
rosa_wichuraiana Rw1G030640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 69, 440, 588, 640
AcuI CTGAAG 2 cut(s) 68, 146
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 312
AgsI TTSAA 6 cut(s) 76, 261, 269, 376, 580, 637
AjnI CCWGG 3 cut(s) 48, 115, 148
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
Alw21I GWGCWC 1 cut(s) 148
AlwNI CAGNNNCTG 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 1 cut(s) 680
ApeKI GCWGC 2 cut(s) 79, 494
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 2 cut(s) 133, 185
AsuHPI GGTGA 2 cut(s) 244, 500
AvaI CYCGRG 1 cut(s) 452
AvaII GGWCC 2 cut(s) 133, 185
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 2 cut(s) 66, 506
BciT130I CCWGG 3 cut(s) 50, 117, 150
BfmI CTRYAG 3 cut(s) 31, 483, 734
BfoI RGCGCY 1 cut(s) 49
BisI GCNGC 3 cut(s) 80, 441, 495
BlpI GCTNAGC 1 cut(s) 42
BlsI GCNGC 3 cut(s) 81, 442, 496
Bme1390I CCNGG 3 cut(s) 50, 117, 150
Bme18I GGWCC 2 cut(s) 133, 185
BmeT110I CYCGRG 1 cut(s) 452
BmgT120I GGNCC 2 cut(s) 133, 185
BmiI GGNNCC 3 cut(s) 121, 186, 240
BmrFI CCNGG 3 cut(s) 50, 117, 150
BmsI GCATC 2 cut(s) 427, 677
Bpu1102I GCTNAGC 1 cut(s) 42
BpuEI CTTGAG 2 cut(s) 209, 507
BsaBI GATNNNNATC 1 cut(s) 700
BsaJI CCNNGG 2 cut(s) 49, 136
Bsc4I CCNNNNNNNGG 1 cut(s) 312
Bse1I ACTGG 1 cut(s) 477
Bse8I GATNNNNATC 1 cut(s) 700
BseBI CCWGG 3 cut(s) 50, 117, 150
BseDI CCNNGG 2 cut(s) 49, 136
BseJI GATNNNNATC 1 cut(s) 700
BseLI CCNNNNNNNGG 1 cut(s) 312
BseMII CTCAG 3 cut(s) 56, 321, 411
BseNI ACTGG 1 cut(s) 477
BseRI GAGGAG 1 cut(s) 112
BseXI GCAGC 2 cut(s) 66, 506
BshFI GGCC 1 cut(s) 682
BsiHKAI GWGCWC 1 cut(s) 148
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 1 cut(s) 20
BslFI GGGAC 2 cut(s) 345, 393
BslI CCNNNNNNNGG 1 cut(s) 312
BsmFI GGGAC 2 cut(s) 345, 393
BsnI GGCC 1 cut(s) 682
BsoBI CYCGRG 1 cut(s) 452
Bsp1286I GDGCHC 1 cut(s) 148
Bsp143I GATC 2 cut(s) 220, 695
Bsp1720I GCTNAGC 1 cut(s) 42
BspACI CCGC 4 cut(s) 69, 440, 588, 640
BspANI GGCC 1 cut(s) 682
BspCNI CTCAG 3 cut(s) 55, 320, 410
BspLI GGNNCC 3 cut(s) 121, 186, 240
BspMAI CTGCAG 1 cut(s) 738
BsrI ACTGG 1 cut(s) 477
BssECI CCNNGG 2 cut(s) 49, 136
BssMI GATC 2 cut(s) 220, 695
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 3 cut(s) 50, 117, 150
Bst4CI ACNGT 2 cut(s) 274, 657
BstC8I GCNNGC 3 cut(s) 64, 464, 468
BstDEI CTNAG 3 cut(s) 42, 307, 397
BstENI CCTNNNNNAGG 1 cut(s) 310
BstH2I RGCGCY 1 cut(s) 49
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 2 cut(s) 223, 698
BstMBI GATC 2 cut(s) 220, 695
BstMWI GCNNNNNNNGC 4 cut(s) 68, 103, 152, 500
BstNI CCWGG 3 cut(s) 50, 117, 150
BstNSI RCATGY 1 cut(s) 466
BstSCI CCNGG 3 cut(s) 48, 115, 148
BstSFI CTRYAG 3 cut(s) 31, 483, 734
BstV1I GCAGC 2 cut(s) 66, 506
BsuRI GGCC 1 cut(s) 682
BtsIMutI CAGTG 2 cut(s) 470, 653
Cac8I GCNNGC 3 cut(s) 64, 464, 468
CaiI CAGNNNCTG 1 cut(s) 49
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 2 cut(s) 133, 185
Csp6I GTAC 1 cut(s) 100
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 1 cut(s) 463
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 3 cut(s) 42, 307, 397
DpnI GATC 2 cut(s) 222, 697
DpnII GATC 2 cut(s) 220, 695
EciI GGCGGA 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 682
Eco47I GGWCC 2 cut(s) 133, 185
Eco57I CTGAAG 2 cut(s) 68, 146
Eco88I CYCGRG 1 cut(s) 452
EcoNI CCTNNNNNAGG 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 185
EcoRII CCWGG 3 cut(s) 48, 115, 148
EcoT14I CCWWGG 1 cut(s) 136
EcoT22I ATGCAT 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 1 cut(s) 466
FaiI YATR 9 cut(s) 57, 110, 248, 290, 404, 464, 602, 724, 754
FalI AAGNNNNNCTT 4 cut(s) 8, 40, 522, 554
FaqI GGGAC 2 cut(s) 345, 393
FatI CATG 1 cut(s) 462
Fnu4HI GCNGC 3 cut(s) 80, 441, 495
Fsp4HI GCNGC 3 cut(s) 80, 441, 495
GlaI GCGC 1 cut(s) 47
GluI GCNGC 3 cut(s) 80, 441, 495
HaeII RGCGCY 1 cut(s) 49
HaeIII GGCC 1 cut(s) 682
HapII CCGG 1 cut(s) 20
HhaI GCGC 1 cut(s) 48
Hin1II CATG 1 cut(s) 466
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 1 cut(s) 257
HpaI GTTAAC 1 cut(s) 357
HpaII CCGG 1 cut(s) 20
HphI GGTGA 2 cut(s) 244, 500
Hpy166II GTNNAC 1 cut(s) 357
Hpy188I TCNGA 4 cut(s) 87, 177, 400, 562
Hpy188III TCNNGA 3 cut(s) 131, 226, 705
Hpy8I GTNNAC 1 cut(s) 357
HpyAV CCTTC 4 cut(s) 82, 223, 229, 370
HpyCH4III ACNGT 2 cut(s) 274, 657
HpyCH4V TGCA 5 cut(s) 250, 466, 628, 648, 736
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 103, 152, 500
HpyF3I CTNAG 3 cut(s) 42, 307, 397
Hsp92II CATG 1 cut(s) 466
HspAI GCGC 1 cut(s) 46
KspAI GTTAAC 1 cut(s) 357
Kzo9I GATC 2 cut(s) 220, 695
LmnI GCTCC 5 cut(s) 71, 125, 151, 300, 545
Lsp1109I GCAGC 2 cut(s) 66, 506
LweI GCATC 2 cut(s) 427, 677
MaeIII GTNAC 2 cut(s) 517, 738
MalI GATC 2 cut(s) 222, 697
MboI GATC 2 cut(s) 220, 695
MboII GAAGA 2 cut(s) 542, 632
MhlI GDGCHC 1 cut(s) 148
MluCI AATT 1 cut(s) 348
MmeI TCCRAC 1 cut(s) 523
MnlI CCTC 5 cut(s) 133, 316, 422, 461, 672
Mph1103I ATGCAT 1 cut(s) 252
MseI TTAA 2 cut(s) 356, 567
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 3 cut(s) 50, 117, 150
MvaI CCWGG 3 cut(s) 50, 117, 150
MwoI GCNNNNNNNGC 4 cut(s) 68, 103, 152, 500
NdeII GATC 2 cut(s) 220, 695
NlaIII CATG 1 cut(s) 466
NlaIV GGNNCC 3 cut(s) 121, 186, 240
NmuCI GTSAC 1 cut(s) 738
NsiI ATGCAT 1 cut(s) 252
NspI RCATGY 1 cut(s) 466
PaeI GCATGC 1 cut(s) 466
PaeR7I CTCGAG 1 cut(s) 452
PceI AGGCCT 1 cut(s) 682
PfeI GAWTC 1 cut(s) 257
PkrI GCNGC 3 cut(s) 81, 442, 496
PpuMI RGGWCCY 1 cut(s) 185
Psp5II RGGWCCY 1 cut(s) 185
Psp6I CCWGG 3 cut(s) 48, 115, 148
PspGI CCWGG 3 cut(s) 48, 115, 148
PspN4I GGNNCC 3 cut(s) 121, 186, 240
PspPI GGNCC 2 cut(s) 133, 185
PspPPI RGGWCCY 1 cut(s) 185
PspXI VCTCGAGB 1 cut(s) 452
PstI CTGCAG 1 cut(s) 738
PstNI CAGNNNCTG 1 cut(s) 49
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SaqAI TTAA 2 cut(s) 356, 567
SatI GCNGC 3 cut(s) 80, 441, 495
Sau3AI GATC 2 cut(s) 220, 695
Sau96I GGNCC 2 cut(s) 133, 185
ScrFI CCNGG 3 cut(s) 50, 117, 150
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 2 cut(s) 427, 677
SfcI CTRYAG 3 cut(s) 31, 483, 734
Sfr274I CTCGAG 1 cut(s) 452
SinI GGWCC 2 cut(s) 133, 185
SlaI CTCGAG 1 cut(s) 452
SmlI CTYRAG 3 cut(s) 224, 452, 522
SmoI CTYRAG 3 cut(s) 224, 452, 522
SphI GCATGC 1 cut(s) 466
Sse9I AATT 1 cut(s) 348
SseBI AGGCCT 1 cut(s) 682
SsiI CCGC 4 cut(s) 69, 440, 588, 640
StuI AGGCCT 1 cut(s) 682
StyD4I CCNGG 3 cut(s) 48, 115, 148
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 2 cut(s) 274, 657
TaqI TCGA 2 cut(s) 366, 453
TasI AATT 1 cut(s) 348
TatI WGTACW 1 cut(s) 99
TauI GCSGC 1 cut(s) 443
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 2 cut(s) 356, 567
Tru9I TTAA 2 cut(s) 356, 567
TscAI CASTG 2 cut(s) 477, 660
TseFI GTSAC 1 cut(s) 738
TseI GCWGC 2 cut(s) 79, 494
Tsp45I GTSAC 1 cut(s) 738
TspDTI ATGAA 1 cut(s) 305
TspRI CASTG 2 cut(s) 477, 660
VpaK11BI GGWCC 2 cut(s) 133, 185
XagI CCTNNNNNAGG 1 cut(s) 310
XceI RCATGY 1 cut(s) 466
XhoI CTCGAG 1 cut(s) 452
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.