pycom07g18770

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0011170
Physical Location & Seq
Forward (+)
21147752 .. 21148685
934 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g18770.1

Sequence Viewer

Length: 426 bp
ATGAAACTCCCCAGAGCCCATGTCAGGGAACTATCTCCACAGCAGAAAGGGTTTTCTGTGGTACTCTCGGATATGTGTCCCCTCGTTTCTGGAATCTCAAGTAAAGATGCAGCTTTATCGCTCGAGTTAGGTATGCAAGCACTGAATTTGGCTGTTGGTGGAGCTGCCTTAGCTCATTGCAATAACGAAGTTCAAACAGAGGAAAGTAATGATGGGGCAACTATAATTTCAGATGATAACGGTGTATTGCGAAGGGGAGGCAATCTTGTGATAAAGCTTTTAGAGAGTGAAGATGTGCAAGAAATCAACAAGATTTGCAAACCGCTGTTTAGAAAGGCATCGCTGTTGAGGCCTAAAGCTACAAGATCATCGTCAAGAGAGATTTATTTGATATGTCAAGGTCTGCAGTCGCAGGCAAAGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

142

Amino Acids

15.2

Weight (kDa)

8.4

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 9 - 134 1.7e-15 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13830
fragaria_vesca FvH4_7g22770
malus_domestica MD07G1200600.v1.1
prunus_persica Prupe.2G237400_v2.0.a1
pyrus_communis pycom01g15670 pycom07g18760 pycom07g18770
rosa_chinensis RchiOBHm_Chr1g0367871
rosa_laevigata RLG00000027260
rosa_multiflora Rmu_co8050104.1_g000001 Rmu_sc0010008.1_g000001 Rmu_ssc0000395.1_g000039
rosa_roxburghii Rroxscaffold_4G00289170
rosa_rugosa Rorug01G0337300 Rorug01G0337400
rosa_samantha Rh1AG344200 Rh1BG306200 Rh1CG321800 Rh1DG338400
rosa_wichuraiana Rw1G030640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 323
AcsI RAATTY 1 cut(s) 145
AfaI GTAC 1 cut(s) 63
AfiI CCNNNNNNNGG 2 cut(s) 24, 25
AgsI TTSAA 1 cut(s) 194
AluBI AGCT 5 cut(s) 113, 164, 173, 277, 359
AluI AGCT 5 cut(s) 113, 164, 173, 277, 359
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 1 cut(s) 350
ApeKI GCWGC 2 cut(s) 110, 164
ApoI RAATTY 1 cut(s) 145
AvaI CYCGRG 1 cut(s) 122
BanII GRGCYC 1 cut(s) 19
BbvI GCAGC 2 cut(s) 122, 151
BccI CCATC 1 cut(s) 206
BcgI CGANNNNNNTGC 2 cut(s) 99, 133
BfmI CTRYAG 1 cut(s) 404
BisI GCNGC 2 cut(s) 111, 165
BlsI GCNGC 2 cut(s) 112, 166
BmeT110I CYCGRG 1 cut(s) 122
BmsI GCATC 2 cut(s) 97, 347
Bpu10I CCTNAGC 1 cut(s) 169
BpuEI CTTGAG 1 cut(s) 82
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 25
Bse3DI GCAATG 1 cut(s) 175
BseLI CCNNNNNNNGG 2 cut(s) 24, 25
BseMI GCAATG 1 cut(s) 175
BseXI GCAGC 2 cut(s) 122, 151
BshFI GGCC 1 cut(s) 352
BsiHKCI CYCGRG 1 cut(s) 122
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 2 cut(s) 24, 25
BsmFI GGGAC 1 cut(s) 63
BsnI GGCC 1 cut(s) 352
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 1 cut(s) 365
BspACI CCGC 1 cut(s) 323
BspANI GGCC 1 cut(s) 352
BspMAI CTGCAG 1 cut(s) 408
BsrDI GCAATG 1 cut(s) 175
BssMI GATC 1 cut(s) 365
Bst4CI ACNGT 1 cut(s) 242
BstC8I GCNNGC 2 cut(s) 138, 414
BstDEI CTNAG 2 cut(s) 169, 423
BstKTI GATC 1 cut(s) 368
BstMBI GATC 1 cut(s) 365
BstMWI GCNNNNNNNGC 2 cut(s) 170, 349
BstSFI CTRYAG 1 cut(s) 404
BstV1I GCAGC 2 cut(s) 122, 151
BsuRI GGCC 1 cut(s) 352
BtgZI GCGATG 1 cut(s) 324
BtsIMutI CAGTG 1 cut(s) 140
Cac8I GCNNGC 2 cut(s) 138, 414
Csp6I GTAC 1 cut(s) 62
CviAII CATG 1 cut(s) 20
CviJI RGCY 8 cut(s) 17, 113, 152, 164, 173, 277, 352, 359
CviKI_1 RGCY 8 cut(s) 17, 113, 152, 164, 173, 277, 352, 359
CviQI GTAC 1 cut(s) 62
DdeI CTNAG 2 cut(s) 169, 423
DpnI GATC 1 cut(s) 367
DpnII GATC 1 cut(s) 365
Eco147I AGGCCT 1 cut(s) 352
Eco24I GRGCYC 1 cut(s) 19
Eco88I CYCGRG 1 cut(s) 122
EcoT38I GRGCYC 1 cut(s) 19
FaeI CATG 1 cut(s) 23
FaiI YATR 5 cut(s) 21, 74, 134, 224, 394
FaqI GGGAC 1 cut(s) 63
FatI CATG 1 cut(s) 19
Fnu4HI GCNGC 2 cut(s) 111, 165
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 2 cut(s) 111, 165
GluI GCNGC 2 cut(s) 111, 165
HaeIII GGCC 1 cut(s) 352
Hin1II CATG 1 cut(s) 23
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 1 cut(s) 93
Hpy188I TCNGA 2 cut(s) 70, 232
Hpy188III TCNNGA 2 cut(s) 90, 375
HpyAV CCTTC 1 cut(s) 246
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4V TGCA 6 cut(s) 110, 136, 180, 298, 318, 406
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 349
HpyF3I CTNAG 2 cut(s) 169, 423
Hsp92II CATG 1 cut(s) 23
Kzo9I GATC 1 cut(s) 365
LmnI GCTCC 1 cut(s) 161
LpnPI CCDG 4 cut(s) 10, 25, 75, 398
Lsp1109I GCAGC 2 cut(s) 122, 151
LweI GCATC 2 cut(s) 97, 347
MalI GATC 1 cut(s) 367
MboI GATC 1 cut(s) 365
MboII GAAGA 1 cut(s) 302
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 2 cut(s) 145, 225
MnlI CCTC 4 cut(s) 92, 193, 251, 342
MspA1I CMGCKG 1 cut(s) 325
MwoI GCNNNNNNNGC 2 cut(s) 170, 349
NdeII GATC 1 cut(s) 365
NlaIII CATG 1 cut(s) 23
PaeR7I CTCGAG 1 cut(s) 122
PceI AGGCCT 1 cut(s) 352
PfeI GAWTC 1 cut(s) 93
PkrI GCNGC 2 cut(s) 112, 166
PspXI VCTCGAGB 1 cut(s) 122
PstI CTGCAG 1 cut(s) 408
RsaI GTAC 1 cut(s) 63
RsaNI GTAC 1 cut(s) 62
SatI GCNGC 2 cut(s) 111, 165
Sau3AI GATC 1 cut(s) 365
SduI GDGCHC 1 cut(s) 19
SetI ASST 7 cut(s) 115, 133, 166, 175, 279, 361, 403
SfaNI GCATC 2 cut(s) 97, 347
SfcI CTRYAG 1 cut(s) 404
Sfr274I CTCGAG 1 cut(s) 122
SlaI CTCGAG 1 cut(s) 122
SmlI CTYRAG 2 cut(s) 97, 122
SmoI CTYRAG 2 cut(s) 97, 122
Sse9I AATT 2 cut(s) 145, 225
SseBI AGGCCT 1 cut(s) 352
SsiI CCGC 1 cut(s) 323
StuI AGGCCT 1 cut(s) 352
TaaI ACNGT 1 cut(s) 242
TaqI TCGA 1 cut(s) 123
TasI AATT 2 cut(s) 145, 225
TfiI GAWTC 1 cut(s) 93
TscAI CASTG 1 cut(s) 147
TseI GCWGC 2 cut(s) 110, 164
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 147
XapI RAATTY 1 cut(s) 145
XhoI CTCGAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.