Rorug01G0337300

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0011170
Physical Location & Seq
Forward (+)
45518280 .. 45519589
1310 bp
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UTR
Exon/CDS
Intron
Rorug01G0337300.1

Sequence Viewer

Length: 468 bp
ATGCATCAGAGCTCCGATTTCGGTGCTCAACGGCCACAGCTACACCGTCAAAAAAGTCAAATTCTCGCCGCACCGGCAAAGCTTGATGATGTCGTGCTCGTACGACTTGTCGTTGCTTCAATACAAGGACTAGGCACTGGCGACGGTTCTGATAGCTACAGCTCCGGCCTTGGATCAATGACTGATCACTTAAGAGCTCAGACTGTGTCTCTGTGCTTGCAGCAAGAGGATGAAGAGGTGCTGGTGCTGCATACGGATTTGGTCGAAGGTCCTCAGCTGATGGAAGTAGCTCAAGTGGAGCGGGCTAGTACAGTTGATAATCAGCCAGTGGAGGAGCCTCTAACCGTGAAATTGACTCGGACAATTGAGAGCTTGTCTAGGTTGAACACCAAAAAGCACTACTCTGACATGTTCATGGTTGGTGGTTATAAATTGTATTCTTCCCTTGACAATCATCGTATGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

155

Amino Acids

17.12

Weight (kDa)

5.42

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13830
fragaria_vesca FvH4_7g22770
malus_domestica MD07G1200600.v1.1
prunus_persica Prupe.2G237400_v2.0.a1
pyrus_communis pycom01g15670 pycom07g18760 pycom07g18770
rosa_chinensis RchiOBHm_Chr1g0367871
rosa_laevigata RLG00000027260
rosa_multiflora Rmu_co8050104.1_g000001 Rmu_sc0010008.1_g000001 Rmu_ssc0000395.1_g000039
rosa_roxburghii Rroxscaffold_4G00289170
rosa_rugosa Rorug01G0337300 Rorug01G0337400
rosa_samantha Rh1AG344200 Rh1BG306200 Rh1CG321800 Rh1DG338400
rosa_wichuraiana Rw1G030640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 429
AccBSI CCGCTC 1 cut(s) 301
AciI CCGC 2 cut(s) 69, 301
AclWI GGATC 1 cut(s) 181
AcoI YGGCCR 1 cut(s) 32
AcsI RAATTY 1 cut(s) 60
AfaI GTAC 2 cut(s) 102, 310
AflII CTTAAG 1 cut(s) 190
AflIII ACRYGT 1 cut(s) 408
AgsI TTSAA 2 cut(s) 120, 385
AluBI AGCT 9 cut(s) 12, 40, 82, 156, 162, 197, 277, 290, 372
AluI AGCT 9 cut(s) 12, 40, 82, 156, 162, 197, 277, 290, 372
Alw21I GWGCWC 4 cut(s) 14, 28, 99, 199
Alw26I GTCTC 1 cut(s) 213
AlwI GGATC 1 cut(s) 181
AoxI GGCC 2 cut(s) 32, 166
ApeKI GCWGC 2 cut(s) 220, 247
ApoI RAATTY 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 269
AvaII GGWCC 1 cut(s) 269
BanII GRGCYC 2 cut(s) 14, 199
Bbv12I GWGCWC 4 cut(s) 14, 28, 99, 199
BbvCI CCTCAGC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 232, 234
BccI CCATC 1 cut(s) 274
BceAI ACGGC 1 cut(s) 47
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BclI TGATCA 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 213
BfaI CTAG 3 cut(s) 131, 306, 378
BfmI CTRYAG 1 cut(s) 157
BfrI CTTAAG 1 cut(s) 190
BglI GCCNNNNNGGC 1 cut(s) 74
BisI GCNGC 3 cut(s) 69, 221, 248
BlsI GCNGC 3 cut(s) 70, 222, 249
Bme18I GGWCC 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 336
BmsI GCATC 1 cut(s) 13
Bpu10I CCTNAGC 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 169
Bse118I RCCGGY 1 cut(s) 73
Bse1I ACTGG 2 cut(s) 142, 326
BseDI CCNNGG 1 cut(s) 169
BseGI GGATG 1 cut(s) 235
BseMII CTCAG 2 cut(s) 212, 287
BseNI ACTGG 2 cut(s) 142, 326
BseRI GAGGAG 1 cut(s) 347
BseXI GCAGC 2 cut(s) 232, 234
BshFI GGCC 2 cut(s) 34, 168
BsiHKAI GWGCWC 4 cut(s) 14, 28, 99, 199
BsiSI CCGG 2 cut(s) 74, 165
BsiWI CGTACG 1 cut(s) 100
BsmAI GTCTC 1 cut(s) 213
BsnI GGCC 2 cut(s) 34, 168
Bsp1286I GDGCHC 4 cut(s) 14, 28, 99, 199
Bsp143I GATC 2 cut(s) 173, 184
BspACI CCGC 2 cut(s) 69, 301
BspANI GGCC 2 cut(s) 34, 168
BspCNI CTCAG 2 cut(s) 211, 286
BspLI GGNNCC 1 cut(s) 336
BspPI GGATC 1 cut(s) 181
BspTI CTTAAG 1 cut(s) 190
BsrBI CCGCTC 1 cut(s) 301
BsrFI RCCGGY 1 cut(s) 73
BsrI ACTGG 2 cut(s) 142, 326
BssAI RCCGGY 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 169
BssMI GATC 2 cut(s) 173, 184
BssT1I CCWWGG 1 cut(s) 169
Bst4CI ACNGT 5 cut(s) 47, 146, 205, 313, 346
Bst6I CTCTTC 1 cut(s) 228
BstAFI CTTAAG 1 cut(s) 190
BstC8I GCNNGC 2 cut(s) 218, 303
BstDEI CTNAG 2 cut(s) 198, 273
BstF5I GGATG 1 cut(s) 235
BstKTI GATC 2 cut(s) 176, 187
BstMAI GTCTC 1 cut(s) 213
BstMBI GATC 2 cut(s) 173, 184
BstMWI GCNNNNNNNGC 2 cut(s) 74, 247
BstNSI RCATGY 1 cut(s) 412
BstSFI CTRYAG 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 232, 234
BsuRI GGCC 2 cut(s) 34, 168
BtsCI GGATG 1 cut(s) 235
BtsIMutI CAGTG 2 cut(s) 135, 333
Cac8I GCNNGC 2 cut(s) 218, 303
Cfr10I RCCGGY 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 269
Csp6I GTAC 2 cut(s) 101, 309
CviAII CATG 2 cut(s) 409, 415
CviQI GTAC 2 cut(s) 101, 309
DdeI CTNAG 2 cut(s) 198, 273
DpnI GATC 2 cut(s) 175, 186
DpnII GATC 2 cut(s) 173, 184
EaeI YGGCCR 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
Ecl136II GAGCTC 2 cut(s) 12, 197
Eco130I CCWWGG 1 cut(s) 169
Eco24I GRGCYC 2 cut(s) 14, 199
Eco47I GGWCC 1 cut(s) 269
Eco53kI GAGCTC 2 cut(s) 12, 197
EcoICRI GAGCTC 2 cut(s) 12, 197
EcoO109I RGGNCCY 1 cut(s) 269
EcoT14I CCWWGG 1 cut(s) 169
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 14, 199
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 2 cut(s) 412, 418
FaiI YATR 5 cut(s) 252, 410, 416, 429, 461
FatI CATG 2 cut(s) 408, 414
FauI CCCGC 1 cut(s) 294
FbaI TGATCA 1 cut(s) 184
Fnu4HI GCNGC 3 cut(s) 69, 221, 248
FokI GGATG 1 cut(s) 242
FriOI GRGCYC 2 cut(s) 14, 199
Fsp4HI GCNGC 3 cut(s) 69, 221, 248
FspBI CTAG 3 cut(s) 131, 306, 378
GluI GCNGC 3 cut(s) 69, 221, 248
HaeIII GGCC 2 cut(s) 34, 168
HapII CCGG 2 cut(s) 74, 165
Hin1II CATG 2 cut(s) 412, 418
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 1 cut(s) 355
HpaII CCGG 2 cut(s) 74, 165
Hpy188I TCNGA 6 cut(s) 9, 16, 151, 201, 360, 406
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 260
HpyCH4III ACNGT 5 cut(s) 47, 146, 205, 313, 346
HpyCH4V TGCA 3 cut(s) 4, 220, 250
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 247
HpyF3I CTNAG 2 cut(s) 198, 273
Hsp92II CATG 2 cut(s) 412, 418
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 2 cut(s) 173, 184
LmnI GCTCC 4 cut(s) 17, 167, 298, 334
LpnPI CCDG 5 cut(s) 87, 123, 178, 227, 339
Lsp1109I GCAGC 2 cut(s) 232, 234
LweI GCATC 1 cut(s) 13
MaeI CTAG 3 cut(s) 131, 306, 378
MalI GATC 2 cut(s) 175, 186
MbiI CCGCTC 1 cut(s) 301
MboI GATC 2 cut(s) 173, 184
MboII GAAGA 2 cut(s) 245, 432
MfeI CAATTG 1 cut(s) 363
MhlI GDGCHC 4 cut(s) 14, 28, 99, 199
MluCI AATT 4 cut(s) 60, 350, 363, 431
MlyI GAGTC 1 cut(s) 349
MnlI CCTC 5 cut(s) 220, 229, 282, 325, 348
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 191
MslI CAYNNNNRTG 1 cut(s) 413
MspA1I CMGCKG 1 cut(s) 277
MspCI CTTAAG 1 cut(s) 190
MspI CCGG 2 cut(s) 74, 165
MunI CAATTG 1 cut(s) 363
MwoI GCNNNNNNNGC 2 cut(s) 74, 247
NdeII GATC 2 cut(s) 173, 184
NlaIII CATG 2 cut(s) 412, 418
NlaIV GGNNCC 1 cut(s) 336
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 412
PciI ACATGT 1 cut(s) 408
Pfl23II CGTACG 1 cut(s) 100
PflFI GACNNNGTC 1 cut(s) 205
PkrI GCNGC 3 cut(s) 70, 222, 249
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
PpuMI RGGWCCY 1 cut(s) 269
PscI ACATGT 1 cut(s) 408
PsiI TTATAA 1 cut(s) 429
Psp124BI GAGCTC 2 cut(s) 14, 199
Psp5II RGGWCCY 1 cut(s) 269
PspLI CGTACG 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 336
PspPI GGNCC 1 cut(s) 269
PspPPI RGGWCCY 1 cut(s) 269
PsyI GACNNNGTC 1 cut(s) 205
PvuII CAGCTG 1 cut(s) 277
RsaI GTAC 2 cut(s) 102, 310
RsaNI GTAC 2 cut(s) 101, 309
RseI CAYNNNNRTG 1 cut(s) 413
SacI GAGCTC 2 cut(s) 14, 199
SaqAI TTAA 1 cut(s) 191
SatI GCNGC 3 cut(s) 69, 221, 248
Sau3AI GATC 2 cut(s) 173, 184
Sau96I GGNCC 1 cut(s) 269
SchI GAGTC 1 cut(s) 349
SduI GDGCHC 4 cut(s) 14, 28, 99, 199
SfaNI GCATC 1 cut(s) 13
SfcI CTRYAG 1 cut(s) 157
SinI GGWCC 1 cut(s) 269
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 2 cut(s) 190, 291
SmoI CTYRAG 2 cut(s) 190, 291
Sse9I AATT 4 cut(s) 60, 350, 363, 431
SsiI CCGC 2 cut(s) 69, 301
SspMI CTAG 3 cut(s) 131, 306, 378
SstI GAGCTC 2 cut(s) 14, 199
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 5 cut(s) 47, 146, 205, 313, 346
TaqI TCGA 1 cut(s) 264
TasI AATT 4 cut(s) 60, 350, 363, 431
TatI WGTACW 1 cut(s) 308
TauI GCSGC 1 cut(s) 71
Tru1I TTAA 1 cut(s) 191
Tru9I TTAA 1 cut(s) 191
TscAI CASTG 2 cut(s) 142, 333
TseI GCWGC 2 cut(s) 220, 247
TspDTI ATGAA 2 cut(s) 246, 403
TspGWI ACGGA 1 cut(s) 269
TspRI CASTG 2 cut(s) 142, 333
Tth111I GACNNNGTC 1 cut(s) 205
Vha464I CTTAAG 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 269
XapI RAATTY 1 cut(s) 60
XceI RCATGY 1 cut(s) 412
XspI CTAG 3 cut(s) 131, 306, 378
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.