RLG00000027260

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0011170
Physical Location & Seq
Reverse (-)
10096079 .. 10097896
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027260

Sequence Viewer

Length: 798 bp
ATGAGTGGGGTAGGAAAGCCGGACTTTTTCTACAGAGAAGCTCAGCGCCTGGGTTATGTAGCTCGCTCCGCCTTCAAGCTGCTTCAGATACAGAAGCAGTACAAGCTCATAACACCAGGCTCCTCTGTTCTGGACCTTGGTTGTGCTCCTGGTGCTTGGCTTCAGGTGGCTTGTCAGAGCTTGGGTCCTATCAAAAATGGTGGTCTTGTTGTTGGGATTGATCTCAAGAAGGTGAAGGTTCCAGCTATGCATTGTGATTCAAGGGTTCAAACTCTGTGTGCTGATGTTATGAAGCTCCCTAAAACCTCAGTCAGGGAACTTTCTCCACAGAAGGGGTTTTCTGTAATACTCTCAGATATGTGTCCCCTCGTTTCTGGGATAGCAAGTAAAGATGCAGCTTTATCCCTCGAGTTAGGCATGCAAGCACTGGATTTGGCCGTAGGTGAAGCAGCATTAGCTAATCCCAACAATGTAACTCAAGTGGAAGAAAGTCTTGTTGGAGCAACTACAAGTTCAGATGTTAATGGTGTATTGAAAAGGGGCGGTCATCTTGTCATAAAGCTTTTAGAGAGTGAAGATGTGCAAGATTTCAACCGCATTTGCAAACCACTGTTTAGAAAGGCATCATTGTTGAGGCCTAAAGCTACAAGATCATCATCAAGAGAGATTTATTTGATATGTCAAGGAAATTTCAGTTATCTGCAATCATTTATTTCACAGATTGCAGTCTTGAGTCTCAAATCATGGTTTATTCCCTTTAGCTATGCAAAGACTTCGGCTCTCTTTGATCTTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

266

Amino Acids

28.77

Weight (kDa)

9.21

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 19 - 229 6e-40 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13830
fragaria_vesca FvH4_7g22770
malus_domestica MD07G1200600.v1.1
prunus_persica Prupe.2G237400_v2.0.a1
pyrus_communis pycom01g15670 pycom07g18760 pycom07g18770
rosa_chinensis RchiOBHm_Chr1g0367871
rosa_laevigata RLG00000027260
rosa_multiflora Rmu_co8050104.1_g000001 Rmu_sc0010008.1_g000001 Rmu_ssc0000395.1_g000039
rosa_roxburghii Rroxscaffold_4G00289170
rosa_rugosa Rorug01G0337300 Rorug01G0337400
rosa_samantha Rh1AG344200 Rh1BG306200 Rh1CG321800 Rh1DG338400
rosa_wichuraiana Rw1G030640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 69, 543, 595
AcoI YGGCCR 1 cut(s) 435
AcsI RAATTY 1 cut(s) 688
AcuI CTGAAG 2 cut(s) 68, 146
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 2 cut(s) 312, 332
AgsI TTSAA 5 cut(s) 76, 261, 269, 535, 592
AjnI CCWGG 3 cut(s) 48, 115, 148
AjuI GAANNNNNNNTTGG 2 cut(s) 480, 512
Alw21I GWGCWC 1 cut(s) 148
Alw26I GTCTC 1 cut(s) 740
AlwNI CAGNNNCTG 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 407
AoxI GGCC 2 cut(s) 435, 635
ApeKI GCWGC 3 cut(s) 79, 395, 449
ApoI RAATTY 1 cut(s) 688
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 2 cut(s) 133, 185
AsuHPI GGTGA 2 cut(s) 244, 455
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 2 cut(s) 133, 185
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 66, 407, 461
BceAI ACGGC 1 cut(s) 422
BcgI CGANNNNNNTGC 2 cut(s) 756, 790
BciT130I CCWGG 3 cut(s) 50, 117, 150
BcoDI GTCTC 1 cut(s) 740
BfmI CTRYAG 1 cut(s) 31
BfoI RGCGCY 1 cut(s) 49
BisI GCNGC 3 cut(s) 80, 396, 450
BlpI GCTNAGC 1 cut(s) 42
BlsI GCNGC 3 cut(s) 81, 397, 451
Bme1390I CCNGG 3 cut(s) 50, 117, 150
Bme18I GGWCC 2 cut(s) 133, 185
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 2 cut(s) 133, 185
BmiI GGNNCC 3 cut(s) 121, 186, 240
BmrFI CCNGG 3 cut(s) 50, 117, 150
BmsI GCATC 2 cut(s) 382, 632
Bpu1102I GCTNAGC 1 cut(s) 42
BpuEI CTTGAG 3 cut(s) 209, 462, 751
BsaBI GATNNNNATC 1 cut(s) 655
BsaJI CCNNGG 2 cut(s) 49, 136
Bsc4I CCNNNNNNNGG 2 cut(s) 312, 332
Bse1I ACTGG 1 cut(s) 432
Bse8I GATNNNNATC 1 cut(s) 655
BseBI CCWGG 3 cut(s) 50, 117, 150
BseDI CCNNGG 2 cut(s) 49, 136
BseJI GATNNNNATC 1 cut(s) 655
BseLI CCNNNNNNNGG 2 cut(s) 312, 332
BseMII CTCAG 3 cut(s) 56, 321, 366
BseNI ACTGG 1 cut(s) 432
BseRI GAGGAG 1 cut(s) 112
BseXI GCAGC 3 cut(s) 66, 407, 461
BshFI GGCC 2 cut(s) 437, 637
BsiHKAI GWGCWC 1 cut(s) 148
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 1 cut(s) 20
BslFI GGGAC 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 312, 332
BsmAI GTCTC 1 cut(s) 740
BsmFI GGGAC 1 cut(s) 348
BsnI GGCC 2 cut(s) 437, 637
BsoBI CYCGRG 1 cut(s) 407
Bsp1286I GDGCHC 1 cut(s) 148
Bsp143I GATC 3 cut(s) 220, 650, 787
Bsp1720I GCTNAGC 1 cut(s) 42
BspACI CCGC 3 cut(s) 69, 543, 595
BspANI GGCC 2 cut(s) 437, 637
BspCNI CTCAG 3 cut(s) 55, 320, 365
BspLI GGNNCC 3 cut(s) 121, 186, 240
BsrI ACTGG 1 cut(s) 432
BssECI CCNNGG 2 cut(s) 49, 136
BssMI GATC 3 cut(s) 220, 650, 787
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 3 cut(s) 50, 117, 150
Bst4CI ACNGT 1 cut(s) 612
BstC8I GCNNGC 3 cut(s) 64, 419, 423
BstDEI CTNAG 3 cut(s) 42, 307, 352
BstENI CCTNNNNNAGG 1 cut(s) 310
BstH2I RGCGCY 1 cut(s) 49
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 3 cut(s) 223, 653, 790
BstMAI GTCTC 1 cut(s) 740
BstMBI GATC 3 cut(s) 220, 650, 787
BstMWI GCNNNNNNNGC 4 cut(s) 68, 103, 152, 455
BstNI CCWGG 3 cut(s) 50, 117, 150
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 3 cut(s) 48, 115, 148
BstSFI CTRYAG 1 cut(s) 31
BstV1I GCAGC 3 cut(s) 66, 407, 461
BsuRI GGCC 2 cut(s) 437, 637
BtsIMutI CAGTG 2 cut(s) 425, 608
Cac8I GCNNGC 3 cut(s) 64, 419, 423
CaiI CAGNNNCTG 1 cut(s) 49
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 2 cut(s) 133, 185
Csp6I GTAC 1 cut(s) 100
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 2 cut(s) 418, 744
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 3 cut(s) 42, 307, 352
DpnI GATC 3 cut(s) 222, 652, 789
DpnII GATC 3 cut(s) 220, 650, 787
EaeI YGGCCR 1 cut(s) 435
EciI GGCGGA 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 637
Eco47I GGWCC 2 cut(s) 133, 185
Eco57I CTGAAG 2 cut(s) 68, 146
Eco88I CYCGRG 1 cut(s) 407
EcoNI CCTNNNNNAGG 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 185
EcoRII CCWGG 3 cut(s) 48, 115, 148
EcoT14I CCWWGG 1 cut(s) 136
EcoT22I ATGCAT 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 2 cut(s) 421, 747
FalI AAGNNNNNCTT 4 cut(s) 8, 40, 477, 509
FaqI GGGAC 1 cut(s) 348
FatI CATG 2 cut(s) 417, 743
Fnu4HI GCNGC 3 cut(s) 80, 396, 450
Fsp4HI GCNGC 3 cut(s) 80, 396, 450
GlaI GCGC 1 cut(s) 47
GluI GCNGC 3 cut(s) 80, 396, 450
HaeII RGCGCY 1 cut(s) 49
HaeIII GGCC 2 cut(s) 437, 637
HapII CCGG 1 cut(s) 20
HhaI GCGC 1 cut(s) 48
Hin1II CATG 2 cut(s) 421, 747
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 2 cut(s) 257, 733
HpaII CCGG 1 cut(s) 20
HphI GGTGA 2 cut(s) 244, 455
Hpy188I TCNGA 4 cut(s) 87, 177, 355, 517
Hpy188III TCNNGA 4 cut(s) 131, 226, 660, 730
HpyAV CCTTC 4 cut(s) 82, 223, 229, 325
HpyCH4III ACNGT 1 cut(s) 612
HpyCH4V TGCA 8 cut(s) 250, 395, 421, 583, 603, 703, 725, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 103, 152, 455
HpyF3I CTNAG 3 cut(s) 42, 307, 352
Hsp92II CATG 2 cut(s) 421, 747
HspAI GCGC 1 cut(s) 46
Kzo9I GATC 3 cut(s) 220, 650, 787
LmnI GCTCC 5 cut(s) 71, 125, 151, 300, 500
Lsp1109I GCAGC 3 cut(s) 66, 407, 461
LweI GCATC 2 cut(s) 382, 632
MaeIII GTNAC 1 cut(s) 472
MalI GATC 3 cut(s) 222, 652, 789
MboI GATC 3 cut(s) 220, 650, 787
MboII GAAGA 2 cut(s) 497, 587
MhlI GDGCHC 1 cut(s) 148
MluCI AATT 1 cut(s) 688
MlyI GAGTC 1 cut(s) 742
MmeI TCCRAC 1 cut(s) 478
MnlI CCTC 5 cut(s) 133, 316, 377, 416, 627
Mph1103I ATGCAT 1 cut(s) 252
MseI TTAA 1 cut(s) 522
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 3 cut(s) 50, 117, 150
MvaI CCWGG 3 cut(s) 50, 117, 150
MwoI GCNNNNNNNGC 4 cut(s) 68, 103, 152, 455
NdeII GATC 3 cut(s) 220, 650, 787
NlaIII CATG 2 cut(s) 421, 747
NlaIV GGNNCC 3 cut(s) 121, 186, 240
NsiI ATGCAT 1 cut(s) 252
NspI RCATGY 1 cut(s) 421
PaeI GCATGC 1 cut(s) 421
PaeR7I CTCGAG 1 cut(s) 407
PceI AGGCCT 1 cut(s) 637
PfeI GAWTC 1 cut(s) 257
PkrI GCNGC 3 cut(s) 81, 397, 451
PleI GAGTC 1 cut(s) 741
PpsI GAGTC 1 cut(s) 741
PpuMI RGGWCCY 1 cut(s) 185
Psp5II RGGWCCY 1 cut(s) 185
Psp6I CCWGG 3 cut(s) 48, 115, 148
PspGI CCWGG 3 cut(s) 48, 115, 148
PspN4I GGNNCC 3 cut(s) 121, 186, 240
PspPI GGNCC 2 cut(s) 133, 185
PspPPI RGGWCCY 1 cut(s) 185
PspXI VCTCGAGB 1 cut(s) 407
PstNI CAGNNNCTG 1 cut(s) 49
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SaqAI TTAA 1 cut(s) 522
SatI GCNGC 3 cut(s) 80, 396, 450
Sau3AI GATC 3 cut(s) 220, 650, 787
Sau96I GGNCC 2 cut(s) 133, 185
SchI GAGTC 1 cut(s) 742
ScrFI CCNGG 3 cut(s) 50, 117, 150
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 2 cut(s) 382, 632
SfcI CTRYAG 1 cut(s) 31
Sfr274I CTCGAG 1 cut(s) 407
SinI GGWCC 2 cut(s) 133, 185
SlaI CTCGAG 1 cut(s) 407
SmlI CTYRAG 4 cut(s) 224, 407, 477, 730
SmoI CTYRAG 4 cut(s) 224, 407, 477, 730
SphI GCATGC 1 cut(s) 421
Sse9I AATT 1 cut(s) 688
SseBI AGGCCT 1 cut(s) 637
SsiI CCGC 3 cut(s) 69, 543, 595
StuI AGGCCT 1 cut(s) 637
StyD4I CCNGG 3 cut(s) 48, 115, 148
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 612
TaqI TCGA 1 cut(s) 408
TasI AATT 1 cut(s) 688
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TscAI CASTG 2 cut(s) 432, 615
TseI GCWGC 3 cut(s) 79, 395, 449
TspDTI ATGAA 1 cut(s) 305
TspRI CASTG 2 cut(s) 432, 615
VpaK11BI GGWCC 2 cut(s) 133, 185
XagI CCTNNNNNAGG 1 cut(s) 310
XapI RAATTY 1 cut(s) 688
XceI RCATGY 1 cut(s) 421
XhoI CTCGAG 1 cut(s) 407
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.