pycom07g18760

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0011170
Physical Location & Seq
Forward (+)
21147192 .. 21147698
507 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g18760.1

Sequence Viewer

Length: 237 bp
ATGAGTGGGGCAGGAAAACCAGATTTCTTCTACAGAGAGGCTCAACGCCTGGGCTATGTGGCTCGCTCGGCCTTCAAGCTGCTTCAGATGCAGAAACAGTACAAGCTCATAACCCCTGGCTCCTCTGTTCTCGACCTCGGCTGTGCTCCCGGCGCTTGGCTTCAGGTGGCTTGCCAGAGCTTGGGTCCCCTAAAAAATGGCGGCCTTGTTGTCGGGATTGATCGATCAAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

79

Amino Acids

8.47

Weight (kDa)

10.02

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 19 - 74 8e-17 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13830
fragaria_vesca FvH4_7g22770
malus_domestica MD07G1200600.v1.1
prunus_persica Prupe.2G237400_v2.0.a1
pyrus_communis pycom01g15670 pycom07g18760 pycom07g18770
rosa_chinensis RchiOBHm_Chr1g0367871
rosa_laevigata RLG00000027260
rosa_multiflora Rmu_co8050104.1_g000001 Rmu_sc0010008.1_g000001 Rmu_ssc0000395.1_g000039
rosa_roxburghii Rroxscaffold_4G00289170
rosa_rugosa Rorug01G0337300 Rorug01G0337400
rosa_samantha Rh1AG344200 Rh1BG306200 Rh1CG321800 Rh1DG338400
rosa_wichuraiana Rw1G030640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 181
AciI CCGC 1 cut(s) 201
AcuI CTGAAG 2 cut(s) 68, 146
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 2 cut(s) 156, 181
AgsI TTSAA 1 cut(s) 76
AjnI CCWGG 2 cut(s) 48, 115
AluBI AGCT 3 cut(s) 79, 106, 180
AluI AGCT 3 cut(s) 79, 106, 180
Alw21I GWGCWC 1 cut(s) 148
AoxI GGCC 2 cut(s) 69, 202
ApeKI GCWGC 1 cut(s) 79
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 185
AsuC2I CCSGG 1 cut(s) 150
AvaII GGWCC 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 1 cut(s) 66
BciT130I CCWGG 2 cut(s) 50, 117
BcnI CCSGG 1 cut(s) 150
BfmI CTRYAG 1 cut(s) 31
BfoI RGCGCY 1 cut(s) 156
BisI GCNGC 2 cut(s) 80, 202
BlsI GCNGC 2 cut(s) 81, 203
Bme1390I CCNGG 3 cut(s) 50, 117, 150
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 3 cut(s) 121, 186, 187
BmrFI CCNGG 3 cut(s) 50, 117, 150
BmsI GCATC 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 150
Bsa29I ATCGAT 1 cut(s) 223
BsaJI CCNNGG 3 cut(s) 49, 115, 136
Bsc4I CCNNNNNNNGG 2 cut(s) 156, 181
BseBI CCWGG 2 cut(s) 50, 117
BseCI ATCGAT 1 cut(s) 223
BseDI CCNNGG 3 cut(s) 49, 115, 136
BseLI CCNNNNNNNGG 2 cut(s) 156, 181
BseRI GAGGAG 1 cut(s) 112
BseXI GCAGC 1 cut(s) 66
BshFI GGCC 2 cut(s) 71, 204
BshVI ATCGAT 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 148
BsiSI CCGG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 171
BslI CCNNNNNNNGG 2 cut(s) 156, 181
BsmFI GGGAC 1 cut(s) 171
BsnI GGCC 2 cut(s) 71, 204
Bsp1286I GDGCHC 1 cut(s) 148
Bsp143I GATC 2 cut(s) 220, 224
BspACI CCGC 1 cut(s) 201
BspANI GGCC 2 cut(s) 71, 204
BspDI ATCGAT 1 cut(s) 223
BspLI GGNNCC 3 cut(s) 121, 186, 187
BssECI CCNNGG 3 cut(s) 49, 115, 136
BssMI GATC 2 cut(s) 220, 224
Bst2UI CCWGG 2 cut(s) 50, 117
Bst4CI ACNGT 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 64, 172
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 2 cut(s) 223, 227
BstMBI GATC 2 cut(s) 220, 224
BstMWI GCNNNNNNNGC 3 cut(s) 68, 88, 152
BstNI CCWGG 2 cut(s) 50, 117
BstSCI CCNGG 3 cut(s) 48, 115, 148
BstSFI CTRYAG 1 cut(s) 31
BstV1I GCAGC 1 cut(s) 66
Bsu15I ATCGAT 1 cut(s) 223
BsuRI GGCC 2 cut(s) 71, 204
BsuTUI ATCGAT 1 cut(s) 223
Cac8I GCNNGC 2 cut(s) 64, 172
CfoI GCGC 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 185
ClaI ATCGAT 1 cut(s) 223
Csp6I GTAC 1 cut(s) 100
CviQI GTAC 1 cut(s) 100
DpnI GATC 2 cut(s) 222, 226
DpnII GATC 2 cut(s) 220, 224
Eco47I GGWCC 1 cut(s) 185
Eco57I CTGAAG 2 cut(s) 68, 146
EcoO109I RGGNCCY 1 cut(s) 185
EcoRII CCWGG 2 cut(s) 48, 115
FaiI YATR 2 cut(s) 57, 110
FaqI GGGAC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 80, 202
Fsp4HI GCNGC 2 cut(s) 80, 202
GlaI GCGC 1 cut(s) 154
GluI GCNGC 2 cut(s) 80, 202
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 2 cut(s) 71, 204
HapII CCGG 1 cut(s) 150
HhaI GCGC 1 cut(s) 155
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HpaII CCGG 1 cut(s) 150
Hpy188I TCNGA 1 cut(s) 87
Hpy188III TCNNGA 3 cut(s) 131, 214, 228
HpyAV CCTTC 2 cut(s) 82, 225
HpyCH4III ACNGT 1 cut(s) 99
HpyCH4V TGCA 1 cut(s) 91
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 88, 152
HspAI GCGC 1 cut(s) 153
KflI GGGWCCC 1 cut(s) 185
Kzo9I GATC 2 cut(s) 220, 224
LmnI GCTCC 2 cut(s) 125, 151
LpnPI CCDG 8 cut(s) 33, 35, 62, 102, 129, 149, 163, 188
Lsp1109I GCAGC 1 cut(s) 66
LweI GCATC 1 cut(s) 78
MalI GATC 2 cut(s) 222, 226
MboI GATC 2 cut(s) 220, 224
MboII GAAGA 1 cut(s) 19
MhlI GDGCHC 1 cut(s) 148
MnlI CCTC 3 cut(s) 31, 133, 146
MspI CCGG 1 cut(s) 150
MspR9I CCNGG 3 cut(s) 50, 117, 150
MvaI CCWGG 2 cut(s) 50, 117
MwoI GCNNNNNNNGC 3 cut(s) 68, 88, 152
NciI CCSGG 1 cut(s) 150
NdeII GATC 2 cut(s) 220, 224
NlaIV GGNNCC 3 cut(s) 121, 186, 187
NmeAIII GCCGAG 2 cut(s) 47, 117
PflMI CCANNNNNTGG 1 cut(s) 181
PkrI GCNGC 2 cut(s) 81, 203
PpuMI RGGWCCY 1 cut(s) 185
Psp5II RGGWCCY 1 cut(s) 185
Psp6I CCWGG 2 cut(s) 48, 115
PspGI CCWGG 2 cut(s) 48, 115
PspN4I GGNNCC 3 cut(s) 121, 186, 187
PspPI GGNCC 1 cut(s) 185
PspPPI RGGWCCY 1 cut(s) 185
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SatI GCNGC 2 cut(s) 80, 202
Sau3AI GATC 2 cut(s) 220, 224
Sau96I GGNCC 1 cut(s) 185
ScrFI CCNGG 3 cut(s) 50, 117, 150
SduI GDGCHC 1 cut(s) 148
SetI ASST 6 cut(s) 81, 108, 138, 168, 182, 236
SfaNI GCATC 1 cut(s) 78
SfcI CTRYAG 1 cut(s) 31
SinI GGWCC 1 cut(s) 185
SsiI CCGC 1 cut(s) 201
StyD4I CCNGG 3 cut(s) 48, 115, 148
TaaI ACNGT 1 cut(s) 99
TaqI TCGA 2 cut(s) 132, 223
TatI WGTACW 1 cut(s) 99
TauI GCSGC 1 cut(s) 204
TseI GCWGC 1 cut(s) 79
Van91I CCANNNNNTGG 1 cut(s) 181
VpaK11BI GGWCC 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.