MD07G1200600.v1.1

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011170
Physical Location & Seq
Forward (+)
27854437 .. 27856207
1771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1200600.v1.1.491

Sequence Viewer

Length: 711 bp
ATGAGTGGGGCAGGAAAACCAGATTTCTTCTACAGAGAGGCTCAACGCCTGGGCTATGTGGCTCGCTCGGCCTTCAAGCTGCTTCAGATGCAGAAACAGTACAAGCTCATAACCCCTGGCTCCTCTGTTCTCGACCTCGGCTGTGCTCCCGGCGCTTGGCTTCAGGTGGCTTGCCAGAGCTTGGGTCCCCTCAAAAATGGCGGCCTTGTTGTTGGGATTGATCTCAAGAAGGTGAAGGTTCCAGCTGAGCATTGTGATTCGAGGGTTCAAACTGTTTGCGCCGACGTCATGAAACTCCCCAGAGCCCATGTCAGGGAACTATCTCCACAGAAAGGGTTTTCTGTGGTACTCTCGGATATGTGTCCCCTCGTTTCTGGAATCTCAAGTAAAGATGCAGCTTTATCGCTCGAGTTAGGTATGCAAGCACTGAATTTGGCTGTTGGTGGAGCTGCCTTAGCTCATTCCAATAACGAAATTCAAACAGAGGAAAGTAATGATGGGACAACTACAATTTCAGATGATAATGGTGTATTGCGAAGGGGAGGTAATCTTGTGATAAAGCTTTTAGAGAGTGAAGATGTGCAAGAAATCAACAAGATTTGCAAACCGCTGTTTAGAAAGGCATCGTTGTTGAGGCCTAAAGCTACAAGATCATCGTCAAGAGAGATTTATTTGATATGTCAAGGTCTGCAGTCGCAGGCAAAGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

237

Amino Acids

25.4

Weight (kDa)

9.07

Isoelectric Point (pI)

42.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 19 - 229 1e-40 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13830
fragaria_vesca FvH4_7g22770
malus_domestica MD07G1200600.v1.1
prunus_persica Prupe.2G237400_v2.0.a1
pyrus_communis pycom01g15670 pycom07g18760 pycom07g18770
rosa_chinensis RchiOBHm_Chr1g0367871
rosa_laevigata RLG00000027260
rosa_multiflora Rmu_co8050104.1_g000001 Rmu_sc0010008.1_g000001 Rmu_ssc0000395.1_g000039
rosa_roxburghii Rroxscaffold_4G00289170
rosa_rugosa Rorug01G0337300 Rorug01G0337400
rosa_samantha Rh1AG344200 Rh1BG306200 Rh1CG321800 Rh1DG338400
rosa_wichuraiana Rw1G030640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 288
AccB7I CCANNNNNTGG 1 cut(s) 181
AciI CCGC 2 cut(s) 201, 608
AcsI RAATTY 2 cut(s) 430, 474
AcuI CTGAAG 2 cut(s) 68, 146
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 2 cut(s) 101, 348
AfiI CCNNNNNNNGG 5 cut(s) 156, 181, 312, 313, 332
AgsI TTSAA 3 cut(s) 76, 269, 479
AjnI CCWGG 2 cut(s) 48, 115
AluBI AGCT 9 cut(s) 79, 106, 180, 245, 398, 449, 458, 562, 644
AluI AGCT 9 cut(s) 79, 106, 180, 245, 398, 449, 458, 562, 644
Alw21I GWGCWC 1 cut(s) 148
Ama87I CYCGRG 1 cut(s) 407
AoxI GGCC 3 cut(s) 69, 202, 635
ApeKI GCWGC 3 cut(s) 79, 395, 449
ApoI RAATTY 2 cut(s) 430, 474
AspLEI GCGC 2 cut(s) 155, 281
AspS9I GGNCC 1 cut(s) 185
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 1 cut(s) 244
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 307
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 66, 407, 436
BccI CCATC 1 cut(s) 491
BcgI CGANNNNNNTGC 2 cut(s) 384, 418
BciT130I CCWGG 2 cut(s) 50, 117
BcnI CCSGG 1 cut(s) 150
BfmI CTRYAG 2 cut(s) 31, 689
BfoI RGCGCY 1 cut(s) 156
BisI GCNGC 4 cut(s) 80, 202, 396, 450
BlpI GCTNAGC 1 cut(s) 246
BlsI GCNGC 4 cut(s) 81, 203, 397, 451
Bme1390I CCNGG 3 cut(s) 50, 117, 150
Bme18I GGWCC 1 cut(s) 185
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 4 cut(s) 121, 186, 187, 240
BmrFI CCNGG 3 cut(s) 50, 117, 150
BmsI GCATC 3 cut(s) 78, 382, 632
Bpu10I CCTNAGC 1 cut(s) 454
Bpu1102I GCTNAGC 1 cut(s) 246
BpuEI CTTGAG 2 cut(s) 209, 367
BpuMI CCSGG 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 285
BsaJI CCNNGG 3 cut(s) 49, 115, 136
Bsc4I CCNNNNNNNGG 5 cut(s) 156, 181, 312, 313, 332
BseBI CCWGG 2 cut(s) 50, 117
BseDI CCNNGG 3 cut(s) 49, 115, 136
BseLI CCNNNNNNNGG 5 cut(s) 156, 181, 312, 313, 332
BseMII CTCAG 1 cut(s) 237
BseRI GAGGAG 1 cut(s) 112
BseXI GCAGC 3 cut(s) 66, 407, 436
BshFI GGCC 3 cut(s) 71, 204, 637
BsiHKAI GWGCWC 1 cut(s) 148
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 1 cut(s) 150
BslFI GGGAC 3 cut(s) 171, 348, 514
BslI CCNNNNNNNGG 5 cut(s) 156, 181, 312, 313, 332
BsmFI GGGAC 3 cut(s) 171, 348, 514
BsnI GGCC 3 cut(s) 71, 204, 637
BsoBI CYCGRG 1 cut(s) 407
Bsp1286I GDGCHC 2 cut(s) 148, 307
Bsp143I GATC 2 cut(s) 220, 650
Bsp1720I GCTNAGC 1 cut(s) 246
BspACI CCGC 2 cut(s) 201, 608
BspANI GGCC 3 cut(s) 71, 204, 637
BspCNI CTCAG 1 cut(s) 238
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 4 cut(s) 121, 186, 187, 240
BspMAI CTGCAG 1 cut(s) 693
BssECI CCNNGG 3 cut(s) 49, 115, 136
BssMI GATC 2 cut(s) 220, 650
BssNI GRCGYC 1 cut(s) 285
Bst2UI CCWGG 2 cut(s) 50, 117
Bst4CI ACNGT 2 cut(s) 99, 274
BstACI GRCGYC 1 cut(s) 285
BstC8I GCNNGC 4 cut(s) 64, 172, 423, 699
BstDEI CTNAG 3 cut(s) 246, 454, 708
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 2 cut(s) 155, 281
BstKTI GATC 2 cut(s) 223, 653
BstMBI GATC 2 cut(s) 220, 650
BstMWI GCNNNNNNNGC 4 cut(s) 68, 88, 152, 455
BstNI CCWGG 2 cut(s) 50, 117
BstSCI CCNGG 3 cut(s) 48, 115, 148
BstSFI CTRYAG 2 cut(s) 31, 689
BstV1I GCAGC 3 cut(s) 66, 407, 436
BsuRI GGCC 3 cut(s) 71, 204, 637
BtsIMutI CAGTG 1 cut(s) 425
Cac8I GCNNGC 4 cut(s) 64, 172, 423, 699
CciI TCATGA 1 cut(s) 288
CfoI GCGC 2 cut(s) 155, 281
Cfr13I GGNCC 1 cut(s) 185
Csp6I GTAC 2 cut(s) 100, 347
CviAII CATG 2 cut(s) 289, 308
CviQI GTAC 2 cut(s) 100, 347
DdeI CTNAG 3 cut(s) 246, 454, 708
DpnI GATC 2 cut(s) 222, 652
DpnII GATC 2 cut(s) 220, 650
Eco147I AGGCCT 1 cut(s) 637
Eco24I GRGCYC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 185
Eco57I CTGAAG 2 cut(s) 68, 146
Eco88I CYCGRG 1 cut(s) 407
EcoO109I RGGNCCY 1 cut(s) 185
EcoRII CCWGG 2 cut(s) 48, 115
EcoT38I GRGCYC 1 cut(s) 307
FaeI CATG 2 cut(s) 292, 311
FaiI YATR 7 cut(s) 57, 110, 290, 309, 359, 419, 679
FaqI GGGAC 3 cut(s) 171, 348, 514
FatI CATG 2 cut(s) 288, 307
Fnu4HI GCNGC 4 cut(s) 80, 202, 396, 450
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 4 cut(s) 80, 202, 396, 450
GlaI GCGC 2 cut(s) 154, 280
GluI GCNGC 4 cut(s) 80, 202, 396, 450
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 3 cut(s) 71, 204, 637
HapII CCGG 1 cut(s) 150
HhaI GCGC 2 cut(s) 155, 281
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 2 cut(s) 292, 311
Hin6I GCGC 2 cut(s) 153, 279
HinP1I GCGC 2 cut(s) 153, 279
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 2 cut(s) 257, 378
HpaII CCGG 1 cut(s) 150
HphI GGTGA 1 cut(s) 244
Hpy188I TCNGA 3 cut(s) 87, 355, 517
Hpy188III TCNNGA 5 cut(s) 131, 226, 289, 375, 660
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 4 cut(s) 82, 223, 229, 531
HpyCH4III ACNGT 2 cut(s) 99, 274
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 6 cut(s) 91, 395, 421, 583, 603, 691
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 88, 152, 455
HpyF3I CTNAG 3 cut(s) 246, 454, 708
HpySE526I ACGT 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 2 cut(s) 292, 311
HspAI GCGC 2 cut(s) 153, 279
KflI GGGWCCC 1 cut(s) 185
Kzo9I GATC 2 cut(s) 220, 650
LmnI GCTCC 3 cut(s) 125, 151, 446
Lsp1109I GCAGC 3 cut(s) 66, 407, 436
LweI GCATC 3 cut(s) 78, 382, 632
MaeII ACGT 1 cut(s) 285
MalI GATC 2 cut(s) 222, 652
MboI GATC 2 cut(s) 220, 650
MboII GAAGA 2 cut(s) 19, 587
MhlI GDGCHC 2 cut(s) 148, 307
MluCI AATT 3 cut(s) 430, 474, 510
MnlI CCTC 9 cut(s) 31, 133, 146, 200, 255, 377, 478, 536, 627
MspA1I CMGCKG 2 cut(s) 245, 610
MspI CCGG 1 cut(s) 150
MspR9I CCNGG 3 cut(s) 50, 117, 150
MvaI CCWGG 2 cut(s) 50, 117
MwoI GCNNNNNNNGC 4 cut(s) 68, 88, 152, 455
NciI CCSGG 1 cut(s) 150
NdeII GATC 2 cut(s) 220, 650
NlaIII CATG 2 cut(s) 292, 311
NlaIV GGNNCC 4 cut(s) 121, 186, 187, 240
NmeAIII GCCGAG 2 cut(s) 47, 117
PaeR7I CTCGAG 1 cut(s) 407
PagI TCATGA 1 cut(s) 288
PceI AGGCCT 1 cut(s) 637
PfeI GAWTC 2 cut(s) 257, 378
PflMI CCANNNNNTGG 1 cut(s) 181
PkrI GCNGC 4 cut(s) 81, 203, 397, 451
PpuMI RGGWCCY 1 cut(s) 185
Psp5II RGGWCCY 1 cut(s) 185
Psp6I CCWGG 2 cut(s) 48, 115
PspGI CCWGG 2 cut(s) 48, 115
PspN4I GGNNCC 4 cut(s) 121, 186, 187, 240
PspPI GGNCC 1 cut(s) 185
PspPPI RGGWCCY 1 cut(s) 185
PspXI VCTCGAGB 1 cut(s) 407
PstI CTGCAG 1 cut(s) 693
PvuII CAGCTG 1 cut(s) 245
RsaI GTAC 2 cut(s) 101, 348
RsaNI GTAC 2 cut(s) 100, 347
SatI GCNGC 4 cut(s) 80, 202, 396, 450
Sau3AI GATC 2 cut(s) 220, 650
Sau96I GGNCC 1 cut(s) 185
ScrFI CCNGG 3 cut(s) 50, 117, 150
SduI GDGCHC 2 cut(s) 148, 307
SfaNI GCATC 3 cut(s) 78, 382, 632
SfcI CTRYAG 2 cut(s) 31, 689
Sfr274I CTCGAG 1 cut(s) 407
SinI GGWCC 1 cut(s) 185
SlaI CTCGAG 1 cut(s) 407
SmlI CTYRAG 3 cut(s) 224, 382, 407
SmoI CTYRAG 3 cut(s) 224, 382, 407
Sse9I AATT 3 cut(s) 430, 474, 510
SseBI AGGCCT 1 cut(s) 637
SsiI CCGC 2 cut(s) 201, 608
StuI AGGCCT 1 cut(s) 637
StyD4I CCNGG 3 cut(s) 48, 115, 148
TaaI ACNGT 2 cut(s) 99, 274
TaiI ACGT 1 cut(s) 288
TaqI TCGA 3 cut(s) 132, 260, 408
TasI AATT 3 cut(s) 430, 474, 510
TatI WGTACW 1 cut(s) 99
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 2 cut(s) 257, 378
TscAI CASTG 1 cut(s) 432
TseI GCWGC 3 cut(s) 79, 395, 449
TspDTI ATGAA 1 cut(s) 305
TspRI CASTG 1 cut(s) 432
Van91I CCANNNNNTGG 1 cut(s) 181
VpaK11BI GGWCC 1 cut(s) 185
XapI RAATTY 2 cut(s) 430, 474
XhoI CTCGAG 1 cut(s) 407
ZraI GACGTC 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.