Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0006462

Physical Location & Seq

Genomic Coordinates Forward (+)

18722634 .. 18726345

Gene Structure 3712 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g24230.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGCAAGGCCTCTGCTTGTCTCACCGCTTGGTTACCCCGGCCACTCACCGGTTGTGTCCTGTTCCGGTCATCGGCATTTCAACCAGAACCTCAAGCTCGACACTGTCATGTGTCCCAAAACTGAACTACGTCCCGAGTATCAGATGCTGCTCTCAGAAGAACCAGTCTTTAACAACTGATGCAAAGAAGTTCGAAGAGGCATCAAAGCATGGGAATCTTGTTCCTCTTTACCGGTGCCTCTTCTCTGATCAACTCACGCCGGTTCTTGCATATCGGTGTTTGGTGAAGGAGGATGATCGAGAGGCCCCAAGTTTTCTGTTTGAGTCAGTCGAACAGAGCACTCAGGGGAGGTACAGCATTGTTGGTGCTCAACCAGCAATGGAAATTGTTGCAAAAGAAAATAAGGTGACTGTTCTTGACCATGAAGCAGGCTGTTCTATTGAGCAGTTTGTCGAGGATCCCATGGCGGTTCCGAAACGAATCTCGGATAACTGGAGGCCTCAGCTCATCGATGAACTTCCTGACGCTTTTTGTGGTGGATGGGTTGGATTCTTCTCATACGACACAGTCCGGTACGTGGAGAAGAAGAGACTGCCGTTCTCGAAGGCTCCGGAGGATGACAGGAATCTACCGGACATGCATTTAGGACTGTATGTGGATGTTATTGTCTTTGATCATGTAGAGAAGAAAATCTATGCTATTCATTGGGTGAGGTTAGACCGGTACTCCTCGGTTGAGAAAGCGTATAAAGATGGAGCAAGACGGTTGGAAAATTTAGTGGCTCGAGTCCATGACATAGAGTCGCCAAAGATTTCTCCAGGTGCTTTCAGTCTGGAAACTCAGAAATTTGATCCAGCTCTGACCATCTCCAACATGACGGGCGAGGCATACAAGAACATTGTGAAGCAGGCAAAGGAGCATATTCTGTCCGGGGATATATTCCAAATTGTGCTGAGTCAGCGATTCGAACGGCGTACATTTGCCCATCCGTTTGAAATCTATAGATCCCTGAGGACAGTAAATCCTAGTCCCTATCTGACTTACCTGCAGGCTAGAGGATGCATACTGGTAGCTTCCAGTCCAGAGATTTTAATGAGTGCCAAAAAGAAGAAGATTGTCAACCGGCCATTAGCCGGAACTGCGAGACGAGGCAAGACATTGGAAGAGGACCAGCAGTTGGAGGTTAAGCTTCTGCAGGACGAGAAGCAGTGCGCCGAACACATAATGCTGGTGGACTTAGGACGCAACGATGTTGGGAAGGTCTCAAAGTCTGGCTCTGTTAAGGTGGACAGGTTAATGGACATAGAACGCTACTCGCACGTCATGCATATCAGCTCTACGGTCACCGGAGAGCTTCGTGAGGGTCTCACTTGCTGGGACGCACTCCGAGCTGCACTGCCTGTGGGTACTGTCAGCGGTGCTCCAAAGATGAAGGCCATAGAGCTTATAGATAAGTTGGAAGTGAACAGGCGCGGCCCGTACAGTGGCGGATTTGGAACAATTTCATTCTCCGGTGATATGGACATTGCACTAGCTCTGAGGACCATGGTAATCCCAACAGCACCCCGGTTTGACACAATGTACTTCTACAACAATGGCAACACCCGCCGCGAGTGGGTTGCATATCTCCAAGCTGGTGCAGGAATAGTAGCGGACAGTGACCCAGATGATGAGAATCTAGAGTGCCAAAACAAAGCTGCAGGTCTTGCTCGCGCTATTGACTTAGCCGAGGCTGCATTCGTGGATGTTGAACAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000162 GO:0003674 GO:0003824 GO:0004049 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005950 GO:0006082 GO:0006520 GO:0006568 GO:0006576 GO:0006586 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009308 GO:0009309 GO:0009889 GO:0009987 GO:0010600 GO:0010817 GO:0016053 GO:0016829 GO:0016830 GO:0016833 GO:0018130 GO:0019222 GO:0019438 GO:0019752 GO:0031323 GO:0031326 GO:0032350 GO:0032991 GO:0034641 GO:0042401 GO:0042430 GO:0042435 GO:0043436 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046219 GO:0046394 GO:0046483 GO:0046885 GO:0050789 GO:0050794 GO:0065007 GO:0065008 GO:0071704 GO:0090354 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00400 ko00405 ko01100 ko01110 ko01130 ko01230 ko02024 ko02025 map00400 map00405 map01100 map01110 map01130 map01230 map02024 map02025

Pfam Domains

Protein Families

Anth_synt_I_N Chorismate_bind

Protein Analysis

586

Amino Acids

65.5

Weight (kDa)

6.69

Isoelectric Point (pI)

47.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Anth_synt_I_N	PF04715	83 - 232	1.9e-29	Anthranilate synthase component I, N terminal region
Chorismate_bind	PF00425	295 - 477	1.3e-56	chorismate binding enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006462)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55870 AT3G55870 AT3G55870 AT5G05730 AT5G05730
fragaria_vesca	FvH4_3g39390 FvH4_7g24230 FvH4_7g24230
malus_domestica	MD01G1149800.v1.1 MD07G1216200.v1.1
prunus_persica	Prupe.2G248100_v2.0.a1
pyrus_communis	pycom07g19850 pycom07g19860
rosa_chinensis	RchiOBHm_Chr1g0369821
rosa_laevigata	RLG00000027103
rosa_multiflora	Rmu_sc0007743.1_g000006 Rmu_sc0008140.1_g000006 Rmu_sc0039066.1_g000001
rosa_roxburghii	Rroxscaffold_4G00287360
rosa_rugosa	Rorug01G0352500
rosa_samantha	Rh1AG361000 Rh1BG324700 Rh1CG337900 Rh1DG355100
rosa_wichuraiana	Rw0G015500 Rw1G031780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1053, 1691
AccB1I	GGYRCC	1 cut(s)	234
AccB7I	CCANNNNNTGG	1 cut(s)	1177
AccII	CGCG	3 cut(s)	1473, 1611, 1713
AccIII	TCCGGA	1 cut(s)	610
AciI	CCGC	8 cut(s)	25, 467, 1416, 1473, 1488, 1606, 1609, 1652
AclWI	GGATC	4 cut(s)	452, 465, 845, 999
AcoI	YGGCCR	2 cut(s)	39, 1124
AcsI	RAATTY	2 cut(s)	772, 845
AfaI	GTAC	7 cut(s)	353, 575, 725, 976, 1408, 1481, 1583
AfiI	CCNNNNNNNGG	7 cut(s)	48, 71, 577, 1133, 1177, 1484, 1615
AgeI	ACCGGT	3 cut(s)	48, 231, 720
AgsI	TTSAA	3 cut(s)	81, 995, 1751
AhdI	GACNNNNNGTC	1 cut(s)	799
AjiI	CACGTC	1 cut(s)	1321
AjnI	CCWGG	1 cut(s)	817
Alw21I	GWGCWC	3 cut(s)	341, 370, 1423
Alw26I	GTCTC	5 cut(s)	24, 583, 1138, 1267, 1370
AlwI	GGATC	4 cut(s)	452, 465, 845, 999
AlwNI	CAGNNNCTG	1 cut(s)	147
Ama87I	CYCGRG	2 cut(s)	133, 783
Aor13HI	TCCGGA	1 cut(s)	610
AoxI	GGCC	7 cut(s)	7, 39, 303, 497, 1124, 1434, 1474
ApeKI	GCWGC	4 cut(s)	147, 1391, 1697, 1733
ApoI	RAATTY	2 cut(s)	772, 845
AsiGI	ACCGGT	3 cut(s)	48, 231, 720
Asp700I	GAANNNNTTC	1 cut(s)	1501
AspLEI	GCGC	3 cut(s)	1214, 1473, 1715
AspS9I	GGNCC	4 cut(s)	304, 1168, 1475, 1542
AsuC2I	CCSGG	3 cut(s)	38, 931, 1567
AsuHPI	GGTGA	7 cut(s)	14, 38, 295, 418, 721, 1336, 1526
AsuII	TTCGAA	2 cut(s)	192, 966
AvaI	CYCGRG	2 cut(s)	133, 783
AvaII	GGWCC	2 cut(s)	1168, 1542
AxyI	CCTNAGG	1 cut(s)	1010
BamHI	GGATCC	1 cut(s)	457
BanI	GGYRCC	1 cut(s)	234
Bbv12I	GWGCWC	3 cut(s)	341, 370, 1423
BbvCI	CCTCAGC	1 cut(s)	501
BbvI	GCAGC	4 cut(s)	134, 1378, 1684, 1720
BccI	CCATC	4 cut(s)	534, 746, 872, 993
BceAI	ACGGC	2 cut(s)	580, 986
BcgI	CGANNNNNNTGC	2 cut(s)	1601, 1635
BciT130I	CCWGG	1 cut(s)	819
BclI	TGATCA	2 cut(s)	247, 673
BcnI	CCSGG	3 cut(s)	38, 931, 1567
BcoDI	GTCTC	5 cut(s)	24, 583, 1138, 1267, 1370
BfaI	CTAG	4 cut(s)	1026, 1053, 1532, 1679
BfmI	CTRYAG	4 cut(s)	1000, 1046, 1193, 1698
BfuAI	ACCTGC	2 cut(s)	1053, 1691
BisI	GCNGC	6 cut(s)	148, 1392, 1474, 1609, 1698, 1734
BlsI	GCNGC	6 cut(s)	149, 1393, 1475, 1610, 1699, 1735
Bme1390I	CCNGG	4 cut(s)	38, 819, 931, 1567
Bme18I	GGWCC	2 cut(s)	1168, 1542
BmeRI	GACNNNNNGTC	1 cut(s)	799
BmeT110I	CYCGRG	2 cut(s)	133, 783
BmgBI	CACGTC	1 cut(s)	1321
BmgT120I	GGNCC	4 cut(s)	304, 1168, 1475, 1542
BmiI	GGNNCC	5 cut(s)	236, 306, 459, 471, 609
BmrFI	CCNGG	4 cut(s)	38, 819, 931, 1567
BmsI	GCATC	4 cut(s)	134, 169, 209, 1049
BpmI	CTGGAG	2 cut(s)	514, 801
Bpu10I	CCTNAGC	1 cut(s)	501
Bpu14I	TTCGAA	2 cut(s)	192, 966
BpuEI	CTTGAG	1 cut(s)	76
BpuMI	CCSGG	3 cut(s)	38, 931, 1567
Bsa29I	ATCGAT	1 cut(s)	510
BsaAI	YACGTR	1 cut(s)	577
BsaBI	GATNNNNATC	1 cut(s)	1674
BsaI	GGTCTC	2 cut(s)	1267, 1370
BsaJI	CCNNGG	7 cut(s)	36, 462, 729, 930, 1545, 1565, 1728
BsaWI	WCCGGW	9 cut(s)	48, 64, 231, 570, 610, 631, 720, 1346, 1511
BsaXI	ACNNNNNCTCC	2 cut(s)	710, 740
Bsc4I	CCNNNNNNNGG	7 cut(s)	48, 71, 577, 1133, 1177, 1484, 1615
Bse118I	RCCGGY	5 cut(s)	48, 231, 259, 720, 1122
Bse1I	ACTGG	4 cut(s)	163, 497, 1071, 1077
Bse21I	CCTNAGG	1 cut(s)	1010
Bse3DI	GCAATG	2 cut(s)	384, 1524
Bse8I	GATNNNNATC	1 cut(s)	1674
BseAI	TCCGGA	1 cut(s)	610
BseBI	CCWGG	1 cut(s)	819
BseCI	ATCGAT	1 cut(s)	510
BseDI	CCNNGG	7 cut(s)	36, 462, 729, 930, 1545, 1565, 1728
BseGI	GGATG	7 cut(s)	298, 545, 622, 664, 985, 1064, 1750
BseJI	GATNNNNATC	1 cut(s)	1674
BseLI	CCNNNNNNNGG	7 cut(s)	48, 71, 577, 1133, 1177, 1484, 1615
BseMI	GCAATG	2 cut(s)	384, 1524
BseMII	CTCAG	7 cut(s)	167, 356, 515, 854, 944, 1001, 1529
BseNI	ACTGG	4 cut(s)	163, 497, 1071, 1077
BseRI	GAGGAG	1 cut(s)	718
BseXI	GCAGC	4 cut(s)	134, 1378, 1684, 1720
BseYI	CCCAGC	1 cut(s)	1374
BsgI	GTGCAG	2 cut(s)	1377, 1659
Bsh1236I	CGCG	3 cut(s)	1473, 1611, 1713
BshFI	GGCC	7 cut(s)	9, 41, 305, 499, 1126, 1436, 1476
BshNI	GGYRCC	1 cut(s)	234
BshTI	ACCGGT	3 cut(s)	48, 231, 720
BshVI	ATCGAT	1 cut(s)	510
BsiHKAI	GWGCWC	3 cut(s)	341, 370, 1423
BsiHKCI	CYCGRG	2 cut(s)	133, 783
BslFI	GGGAC	4 cut(s)	98, 116, 1014, 1391
BslI	CCNNNNNNNGG	7 cut(s)	48, 71, 577, 1133, 1177, 1484, 1615
BsmAI	GTCTC	5 cut(s)	24, 583, 1138, 1267, 1370
BsmBI	CGTCTC	1 cut(s)	1138
BsmFI	GGGAC	4 cut(s)	98, 116, 1014, 1391
BsmI	GAATGC	1 cut(s)	1736
BsnI	GGCC	7 cut(s)	9, 41, 305, 499, 1126, 1436, 1476
Bso31I	GGTCTC	2 cut(s)	1267, 1370
BsoBI	CYCGRG	2 cut(s)	133, 783
Bsp119I	TTCGAA	2 cut(s)	192, 966
Bsp1286I	GDGCHC	3 cut(s)	341, 370, 1423
Bsp13I	TCCGGA	1 cut(s)	610
Bsp143I	GATC	6 cut(s)	247, 295, 457, 673, 850, 1004
Bsp19I	CCATGG	2 cut(s)	462, 1545
BspACI	CCGC	8 cut(s)	25, 467, 1416, 1473, 1488, 1606, 1609, 1652
BspANI	GGCC	7 cut(s)	9, 41, 305, 499, 1126, 1436, 1476
BspCNI	CTCAG	7 cut(s)	166, 355, 514, 853, 945, 1002, 1530
BspDI	ATCGAT	1 cut(s)	510
BspEI	TCCGGA	1 cut(s)	610
BspFNI	CGCG	3 cut(s)	1473, 1611, 1713
BspLI	GGNNCC	5 cut(s)	236, 306, 459, 471, 609
BspMAI	CTGCAG	3 cut(s)	1050, 1197, 1702
BspMI	ACCTGC	2 cut(s)	1053, 1691
BspPI	GGATC	4 cut(s)	452, 465, 845, 999
BspT104I	TTCGAA	2 cut(s)	192, 966
BspT107I	GGYRCC	1 cut(s)	234
BspTNI	GGTCTC	2 cut(s)	1267, 1370
BsrDI	GCAATG	2 cut(s)	384, 1524
BsrFI	RCCGGY	5 cut(s)	48, 231, 259, 720, 1122
BsrI	ACTGG	4 cut(s)	163, 497, 1071, 1077
BssAI	RCCGGY	5 cut(s)	48, 231, 259, 720, 1122
BssECI	CCNNGG	7 cut(s)	36, 462, 729, 930, 1545, 1565, 1728
BssMI	GATC	6 cut(s)	247, 295, 457, 673, 850, 1004
BssT1I	CCWWGG	2 cut(s)	462, 1545
Bst2UI	CCWGG	1 cut(s)	819
Bst6I	CTCTTC	4 cut(s)	189, 245, 581, 1158
BstAPI	GCANNNNNTGC	2 cut(s)	1324, 1706
BstBAI	YACGTR	1 cut(s)	577
BstBI	TTCGAA	2 cut(s)	192, 966
BstC8I	GCNNGC	4 cut(s)	430, 909, 1050, 1711
BstDEI	CTNAG	9 cut(s)	153, 342, 501, 840, 953, 1010, 1237, 1538, 1723
BstDSI	CCRYGG	2 cut(s)	462, 1545
BstEII	GGTNACC	2 cut(s)	31, 1342
BstF5I	GGATG	7 cut(s)	298, 545, 622, 664, 985, 1064, 1750
BstFNI	CGCG	3 cut(s)	1473, 1611, 1713
BstHHI	GCGC	3 cut(s)	1214, 1473, 1715
BstKTI	GATC	6 cut(s)	250, 298, 460, 676, 853, 1007
BstMAI	GTCTC	5 cut(s)	24, 583, 1138, 1267, 1370
BstMBI	GATC	6 cut(s)	247, 295, 457, 673, 850, 1004
BstMWI	GCNNNNNNNGC	8 cut(s)	374, 958, 1139, 1324, 1388, 1605, 1706, 1733
BstNI	CCWGG	1 cut(s)	819
BstNSI	RCATGY	1 cut(s)	640
BstPI	GGTNACC	2 cut(s)	31, 1342
BstSCI	CCNGG	4 cut(s)	36, 817, 929, 1565
BstSFI	CTRYAG	4 cut(s)	1000, 1046, 1193, 1698
BstUI	CGCG	3 cut(s)	1473, 1611, 1713
BstV1I	GCAGC	4 cut(s)	134, 1378, 1684, 1720
BstX2I	RGATCY	2 cut(s)	457, 1004
BstYI	RGATCY	2 cut(s)	457, 1004
Bsu15I	ATCGAT	1 cut(s)	510
Bsu36I	CCTNAGG	1 cut(s)	1010
BsuRI	GGCC	7 cut(s)	9, 41, 305, 499, 1126, 1436, 1476
BsuTUI	ATCGAT	1 cut(s)	510
BtgI	CCRYGG	2 cut(s)	462, 1545
BtrI	CACGTC	1 cut(s)	1321
BtsCI	GGATG	7 cut(s)	298, 545, 622, 664, 985, 1064, 1750
BtsI	GCAGTG	2 cut(s)	1214, 1394
BtsIMutI	CAGTG	5 cut(s)	101, 1214, 1394, 1489, 1663
BveI	ACCTGC	2 cut(s)	1053, 1691
Cac8I	GCNNGC	4 cut(s)	430, 909, 1050, 1711
CaiI	CAGNNNCTG	1 cut(s)	147
CfoI	GCGC	3 cut(s)	1214, 1473, 1715
Cfr10I	RCCGGY	5 cut(s)	48, 231, 259, 720, 1122
Cfr13I	GGNCC	4 cut(s)	304, 1168, 1475, 1542
ClaI	ATCGAT	1 cut(s)	510
CseI	GACGC	3 cut(s)	533, 1251, 1388
Csp6I	GTAC	7 cut(s)	352, 574, 724, 975, 1407, 1480, 1582
CspAI	ACCGGT	3 cut(s)	48, 231, 720
CviQI	GTAC	7 cut(s)	352, 574, 724, 975, 1407, 1480, 1582
DdeI	CTNAG	9 cut(s)	153, 342, 501, 840, 953, 1010, 1237, 1538, 1723
DpnI	GATC	6 cut(s)	249, 297, 459, 675, 852, 1006
DpnII	GATC	6 cut(s)	247, 295, 457, 673, 850, 1004
DriI	GACNNNNNGTC	1 cut(s)	799
EaeI	YGGCCR	2 cut(s)	39, 1124
Eam1104I	CTCTTC	4 cut(s)	189, 245, 581, 1158
Eam1105I	GACNNNNNGTC	1 cut(s)	799
EarI	CTCTTC	4 cut(s)	189, 245, 581, 1158
EciI	GGCGGA	1 cut(s)	1503
Eco130I	CCWWGG	2 cut(s)	462, 1545
Eco147I	AGGCCT	2 cut(s)	9, 499
Eco31I	GGTCTC	2 cut(s)	1267, 1370
Eco47I	GGWCC	2 cut(s)	1168, 1542
Eco81I	CCTNAGG	1 cut(s)	1010
Eco88I	CYCGRG	2 cut(s)	133, 783
Eco91I	GGTNACC	2 cut(s)	31, 1342
EcoO109I	RGGNCCY	1 cut(s)	304
EcoO65I	GGTNACC	2 cut(s)	31, 1342
EcoRII	CCWGG	1 cut(s)	817
EcoT14I	CCWWGG	2 cut(s)	462, 1545
EcoT22I	ATGCAT	3 cut(s)	642, 1064, 1329
ErhI	CCWWGG	2 cut(s)	462, 1545
Esp3I	CGTCTC	1 cut(s)	1138
FaqI	GGGAC	4 cut(s)	98, 116, 1014, 1391
FauI	CCCGC	1 cut(s)	1613
FbaI	TGATCA	2 cut(s)	247, 673
Fnu4HI	GCNGC	6 cut(s)	148, 1392, 1474, 1609, 1698, 1734
FokI	GGATG	6 cut(s)	305, 552, 629, 671, 972, 1071
Fsp4HI	GCNGC	6 cut(s)	148, 1392, 1474, 1609, 1698, 1734
FspBI	CTAG	4 cut(s)	1026, 1053, 1532, 1679
GlaI	GCGC	3 cut(s)	1213, 1472, 1714
GluI	GCNGC	6 cut(s)	148, 1392, 1474, 1609, 1698, 1734
GsaI	CCCAGC	1 cut(s)	1378
GsuI	CTGGAG	2 cut(s)	514, 801
HaeIII	GGCC	7 cut(s)	9, 41, 305, 499, 1126, 1436, 1476
HgaI	GACGC	3 cut(s)	533, 1251, 1388
HhaI	GCGC	3 cut(s)	1214, 1473, 1715
Hin6I	GCGC	3 cut(s)	1212, 1471, 1713
HinP1I	GCGC	3 cut(s)	1212, 1471, 1713
HincII	GTYRAC	1 cut(s)	1120
HindII	GTYRAC	1 cut(s)	1120
HindIII	AAGCTT	1 cut(s)	1187
HphI	GGTGA	7 cut(s)	14, 38, 295, 418, 721, 1336, 1526
Hpy166II	GTNNAC	4 cut(s)	1120, 1234, 1288, 1465
Hpy8I	GTNNAC	4 cut(s)	1120, 1234, 1288, 1465
HpyAV	CCTTC	4 cut(s)	280, 598, 1252, 1426
HpyCH4IV	ACGT	3 cut(s)	129, 576, 1320
HpyF10VI	GCNNNNNNNGC	8 cut(s)	374, 958, 1139, 1324, 1388, 1605, 1706, 1733
HpyF3I	CTNAG	9 cut(s)	153, 342, 501, 840, 953, 1010, 1237, 1538, 1723
HpySE526I	ACGT	3 cut(s)	129, 576, 1320
HspAI	GCGC	3 cut(s)	1212, 1471, 1713
Kpn2I	TCCGGA	1 cut(s)	610
Ksp22I	TGATCA	2 cut(s)	247, 673
Kzo9I	GATC	6 cut(s)	247, 295, 457, 673, 850, 1004
LmnI	GCTCC	4 cut(s)	613, 755, 916, 1426
Lsp1109I	GCAGC	4 cut(s)	134, 1378, 1684, 1720
LweI	GCATC	4 cut(s)	134, 169, 209, 1049
MaeI	CTAG	4 cut(s)	1026, 1053, 1532, 1679
MaeII	ACGT	3 cut(s)	129, 576, 1320
MaeIII	GTNAC	4 cut(s)	31, 406, 1342, 1658
MalI	GATC	6 cut(s)	249, 297, 459, 675, 852, 1006
MboI	GATC	6 cut(s)	247, 295, 457, 673, 850, 1004
MflI	RGATCY	2 cut(s)	457, 1004
MhlI	GDGCHC	3 cut(s)	341, 370, 1423
MluCI	AATT	5 cut(s)	384, 772, 845, 945, 1500
MlyI	GAGTC	4 cut(s)	332, 795, 809, 964
MmeI	TCCRAC	5 cut(s)	526, 747, 894, 1158, 1437
Mph1103I	ATGCAT	3 cut(s)	642, 1064, 1329
MroI	TCCGGA	1 cut(s)	610
MroXI	GAANNNNTTC	1 cut(s)	1501
MseI	TTAA	5 cut(s)	170, 1091, 1185, 1281, 1295
MslI	CAYNNNNRTG	2 cut(s)	106, 274
MspA1I	CMGCKG	1 cut(s)	1416
MspR9I	CCNGG	4 cut(s)	38, 819, 931, 1567
Mva1269I	GAATGC	1 cut(s)	1736
MvaI	CCWGG	1 cut(s)	819
MvnI	CGCG	3 cut(s)	1473, 1611, 1713
MwoI	GCNNNNNNNGC	8 cut(s)	374, 958, 1139, 1324, 1388, 1605, 1706, 1733
NciI	CCSGG	3 cut(s)	38, 931, 1567
NcoI	CCATGG	2 cut(s)	462, 1545
NdeII	GATC	6 cut(s)	247, 295, 457, 673, 850, 1004
NlaIV	GGNNCC	5 cut(s)	236, 306, 459, 471, 609
NmeAIII	GCCGAG	1 cut(s)	1753
NmuCI	GTSAC	3 cut(s)	406, 1342, 1658
NsiI	ATGCAT	3 cut(s)	642, 1064, 1329
NspI	RCATGY	1 cut(s)	640
NspV	TTCGAA	2 cut(s)	192, 966
PaeR7I	CTCGAG	1 cut(s)	783
PceI	AGGCCT	2 cut(s)	9, 499
PctI	GAATGC	1 cut(s)	1736
PdmI	GAANNNNTTC	1 cut(s)	1501
PfeI	GAWTC	6 cut(s)	214, 480, 549, 625, 963, 1675
PflFI	GACNNNGTC	2 cut(s)	103, 566
PflMI	CCANNNNNTGG	1 cut(s)	1177
PinAI	ACCGGT	3 cut(s)	48, 231, 720
PkrI	GCNGC	6 cut(s)	149, 1393, 1475, 1610, 1699, 1735
PleI	GAGTC	4 cut(s)	331, 794, 808, 963
PpsI	GAGTC	4 cut(s)	331, 794, 808, 963
Ppu21I	YACGTR	1 cut(s)	577
Psp6I	CCWGG	1 cut(s)	817
PspEI	GGTNACC	2 cut(s)	31, 1342
PspFI	CCCAGC	1 cut(s)	1374
PspGI	CCWGG	1 cut(s)	817
PspN4I	GGNNCC	5 cut(s)	236, 306, 459, 471, 609
PspPI	GGNCC	4 cut(s)	304, 1168, 1475, 1542
PspXI	VCTCGAGB	1 cut(s)	783
PstI	CTGCAG	3 cut(s)	1050, 1197, 1702
PstNI	CAGNNNCTG	1 cut(s)	147
PsuI	RGATCY	2 cut(s)	457, 1004
PsyI	GACNNNGTC	2 cut(s)	103, 566
RsaI	GTAC	7 cut(s)	353, 575, 725, 976, 1408, 1481, 1583
RsaNI	GTAC	7 cut(s)	352, 574, 724, 975, 1407, 1480, 1582
RseI	CAYNNNNRTG	2 cut(s)	106, 274
SaqAI	TTAA	5 cut(s)	170, 1091, 1185, 1281, 1295
SatI	GCNGC	6 cut(s)	148, 1392, 1474, 1609, 1698, 1734
Sau3AI	GATC	6 cut(s)	247, 295, 457, 673, 850, 1004
Sau96I	GGNCC	4 cut(s)	304, 1168, 1475, 1542
SbfI	CCTGCAGG	1 cut(s)	1050
SchI	GAGTC	4 cut(s)	332, 795, 809, 964
ScrFI	CCNGG	4 cut(s)	38, 819, 931, 1567
SdaI	CCTGCAGG	1 cut(s)	1050
SduI	GDGCHC	3 cut(s)	341, 370, 1423
SfaNI	GCATC	4 cut(s)	134, 169, 209, 1049
SfcI	CTRYAG	4 cut(s)	1000, 1046, 1193, 1698
Sfr274I	CTCGAG	1 cut(s)	783
SfuI	TTCGAA	2 cut(s)	192, 966
SinI	GGWCC	2 cut(s)	1168, 1542
SlaI	CTCGAG	1 cut(s)	783
SmiMI	CAYNNNNRTG	2 cut(s)	106, 274
SmlI	CTYRAG	2 cut(s)	91, 783
SmoI	CTYRAG	2 cut(s)	91, 783
Sse8387I	CCTGCAGG	1 cut(s)	1050
Sse9I	AATT	5 cut(s)	384, 772, 845, 945, 1500
SseBI	AGGCCT	2 cut(s)	9, 499
SsiI	CCGC	8 cut(s)	25, 467, 1416, 1473, 1488, 1606, 1609, 1652
SspMI	CTAG	4 cut(s)	1026, 1053, 1532, 1679
StuI	AGGCCT	2 cut(s)	9, 499
StyD4I	CCNGG	4 cut(s)	36, 817, 929, 1565
StyI	CCWWGG	2 cut(s)	462, 1545
TaiI	ACGT	3 cut(s)	132, 579, 1323
TaqI	TCGA	9 cut(s)	98, 192, 298, 330, 453, 510, 602, 784, 966
TasI	AATT	5 cut(s)	384, 772, 845, 945, 1500
TatI	WGTACW	1 cut(s)	1581
TauI	GCSGC	2 cut(s)	1476, 1611
TfiI	GAWTC	6 cut(s)	214, 480, 549, 625, 963, 1675
Tru1I	TTAA	5 cut(s)	170, 1091, 1185, 1281, 1295
Tru9I	TTAA	5 cut(s)	170, 1091, 1185, 1281, 1295
TscAI	CASTG	5 cut(s)	108, 1214, 1401, 1489, 1663
TseFI	GTSAC	3 cut(s)	406, 1342, 1658
TseI	GCWGC	4 cut(s)	147, 1391, 1697, 1733
Tsp45I	GTSAC	3 cut(s)	406, 1342, 1658
TspDTI	ATGAA	5 cut(s)	438, 528, 692, 1445, 1494
TspGWI	ACGGA	1 cut(s)	978
TspRI	CASTG	5 cut(s)	108, 1214, 1401, 1489, 1663
Tth111I	GACNNNGTC	2 cut(s)	103, 566
Van91I	CCANNNNNTGG	1 cut(s)	1177
VpaK11BI	GGWCC	2 cut(s)	1168, 1542
XapI	RAATTY	2 cut(s)	772, 845
XbaI	TCTAGA	1 cut(s)	1678
XceI	RCATGY	1 cut(s)	640
XhoI	CTCGAG	1 cut(s)	783
XmnI	GAANNNNTTC	1 cut(s)	1501
XspI	CTAG	4 cut(s)	1026, 1053, 1532, 1679
Zsp2I	ATGCAT	3 cut(s)	642, 1064, 1329

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.