FvH4_7g24900

Mad3/BUB1 hoMad3/BUB1 homology region 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010450
Physical Location & Seq
Forward (+)
19122081 .. 19124517
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24900.t4

Sequence Viewer

Length: 1293 bp
ATGGATTTTGTGGAGGATCCGAGAGAGCTTTTGAGGACGATGGAGGCGAATCAGATCGGGACGAAGCACCCTATGTTCTACCAGGCGTATGCTCTTTACTATGAGAAGAAAAAGAGGTTTGAGGAGGGTGAGAAAATGTACCATTTGGGGGTGCAGAACCATGCTGATCCCATTGAAGAATTAGAGAAAGCATATGAGCAGTTCCTGCAGCGCATGGAGAGACACAGGAAATGGAAACACCAGGAAAAGAGAACCACAAAAAAAGTATCAGATAGGAGTAGAGTCTCTCGAATTAGTGGAGAAAGGAACATTGAAAAGGAATGCAGGATGGGAGATAGGCCTAGAGAAACCCGGCATGATGGAACTAGAAGTAGGCATCAAGTGACTGCTGGAGGGGTGTCAGATGAACGGAGTATGTGCAGCGACGATACAGTATTGGGGAAGTTTGTAGGCACTGCCATTGTTGGAAAATCTGAAGCAGAAGATGCTTGTCACCATGGACTGGTGGATCCTACTATAAACATGAAAGAGGCCATGAGTGCCATAAATAGTATGTTTCGAGAGCCTTTAGAGATGGCTCCGGTTAGAAGAGGGTCACGCCAATCTCAACAAAAGGAGAACAATACAAACAATGGATTCCAGGTGTTTGTTGATGAAAACTTGGAAAAGGGAGGCAAATCAAAAGATAAAGGTGTGGTGCAGCAAAGTAGAGAACCTTTGAACATTTTCATTGATGATGATCAAGATTGCCTCGAGCAGAGTGAAGTTCAGAATAAGAGAGAAGATTCTTCCCCATCTGCTTCACATGAAAATGCTTTTGTGTTTCTAAATCCGAAAGATGTTCCGAGTGAAAGTCCTAATGGTGAAGGTTCATCTAGACCAAGATTTAGAGAGGATACAATGATGGTTCGTAGGATTGTTGGGACCACCATTTCAGAGGACCCGCAGGTGGAAAACGCTTGCCACCATGGCTTAGTTGAACCAACTATCAACTTAAAGGAGGCCATGGATGATATCAACAACATGTTTGGCAAGCCAATAGATTTCGTAAGAAGCAGAAGAGTTAGAAGGCAGGACAAAGTACCAGAGAAGAAACAAGATCTTGGCGGGTTTTGTATACTTCCTGATGATGAAGACGATAGCGGTGATGATAACTTGGAACAACAACAAGTGCAGAAAATGAAAGATCTACCTGGAAAATCAAAAGATTCTGATCTGTTTGAGACTACTGTTTTCACAAAGCAGGCCATGGATGATATCAATAAGATGTTTGGAATGCCACTGGACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

431

Amino Acids

49.44

Weight (kDa)

5.55

Isoelectric Point (pI)

62.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05510
fragaria_vesca FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900
malus_domestica MD01G1156700.v1.1 MD07G1225400.v1.1
prunus_persica Prupe.2G254200_v2.0.a1
pyrus_communis pycom01g17350 pycom07g20410
rosa_chinensis RchiOBHm_Chr1g0370781
rosa_laevigata RLG00000027010
rosa_multiflora Rmu_sc0009474.1_g000004
rosa_roxburghii Rroxscaffold_4G00286230
rosa_rugosa Rorug01G0359700
rosa_samantha Rh1AG368800 Rh1BG332400 Rh1CG346300 Rh1DG363800
rosa_wichuraiana Rw1G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 937
Acc36I ACCTGC 1 cut(s) 937
AccB7I CCANNNNNTGG 1 cut(s) 502
AccI GTMKAC 1 cut(s) 1117
AciI CCGC 3 cut(s) 944, 1107, 1143
AclWI GGATC 5 cut(s) 11, 24, 161, 503, 516
AcuI CTGAAG 1 cut(s) 495
AfaI GTAC 2 cut(s) 140, 1083
AfiI CCNNNNNNNGG 3 cut(s) 148, 502, 949
AflIII ACRYGT 1 cut(s) 1023
AgsI TTSAA 4 cut(s) 176, 314, 721, 980
AjnI CCWGG 4 cut(s) 81, 240, 639, 1192
AluBI AGCT 1 cut(s) 28
AluI AGCT 1 cut(s) 28
Alw26I GTCTC 3 cut(s) 214, 289, 1217
AlwI GGATC 5 cut(s) 11, 24, 161, 503, 516
AlwNI CAGNNNCTG 1 cut(s) 205
Ama87I CYCGRG 1 cut(s) 752
AoxI GGCC 4 cut(s) 338, 531, 1002, 1245
ApeKI GCWGC 3 cut(s) 208, 420, 700
Asp700I GAANNNNTTC 1 cut(s) 725
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 2 cut(s) 924, 940
AsuC2I CCSGG 1 cut(s) 352
AsuHPI GGTGA 4 cut(s) 140, 485, 875, 1157
AvaI CYCGRG 1 cut(s) 752
AvaII GGWCC 2 cut(s) 924, 940
BamHI GGATCC 2 cut(s) 16, 508
BbsI GAAGAC 1 cut(s) 1140
BbvI GCAGC 3 cut(s) 220, 432, 712
BccI CCATC 6 cut(s) 34, 322, 353, 568, 802, 898
BciT130I CCWGG 4 cut(s) 83, 242, 641, 1194
BciVI GTATCC 1 cut(s) 889
BclI TGATCA 1 cut(s) 739
BcnI CCSGG 1 cut(s) 352
BcoDI GTCTC 3 cut(s) 214, 289, 1217
BfaI CTAG 3 cut(s) 342, 366, 876
BfmI CTRYAG 1 cut(s) 206
BfuAI ACCTGC 1 cut(s) 937
BfuI GTATCC 1 cut(s) 889
BglI GCCNNNNNGGC 1 cut(s) 969
BglII AGATCT 2 cut(s) 1099, 1186
BisI GCNGC 3 cut(s) 209, 421, 701
BlsI GCNGC 3 cut(s) 210, 422, 702
Bme1390I CCNGG 5 cut(s) 83, 242, 352, 641, 1194
Bme18I GGWCC 2 cut(s) 924, 940
BmeT110I CYCGRG 1 cut(s) 752
BmgT120I GGNCC 2 cut(s) 924, 940
BmiI GGNNCC 5 cut(s) 18, 510, 579, 925, 942
BmrFI CCNGG 5 cut(s) 83, 242, 352, 641, 1194
BmsI GCATC 2 cut(s) 385, 475
BpiI GAAGAC 1 cut(s) 1140
BpmI CTGGAG 1 cut(s) 411
BpuMI CCSGG 1 cut(s) 352
BsaBI GATNNNNATC 2 cut(s) 738, 1212
BsaJI CCNNGG 4 cut(s) 496, 967, 1005, 1248
BsaWI WCCGGW 1 cut(s) 580
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 502, 949
Bse1I ACTGG 2 cut(s) 507, 1287
Bse8I GATNNNNATC 2 cut(s) 738, 1212
BseBI CCWGG 4 cut(s) 83, 242, 641, 1194
BseDI CCNNGG 4 cut(s) 496, 967, 1005, 1248
BseGI GGATG 3 cut(s) 333, 1015, 1258
BseJI GATNNNNATC 2 cut(s) 738, 1212
BseLI CCNNNNNNNGG 3 cut(s) 148, 502, 949
BseNI ACTGG 2 cut(s) 507, 1287
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 3 cut(s) 220, 432, 712
BsgI GTGCAG 4 cut(s) 173, 439, 719, 1193
BshFI GGCC 4 cut(s) 340, 533, 1004, 1247
BsiHKCI CYCGRG 1 cut(s) 752
BsiSI CCGG 2 cut(s) 352, 581
BslFI GGGAC 2 cut(s) 73, 937
BslI CCNNNNNNNGG 3 cut(s) 148, 502, 949
BsmAI GTCTC 3 cut(s) 214, 289, 1217
BsmFI GGGAC 2 cut(s) 73, 937
BsmI GAATGC 2 cut(s) 326, 1281
BsnI GGCC 4 cut(s) 340, 533, 1004, 1247
BsoBI CYCGRG 1 cut(s) 752
Bsp143I GATC 8 cut(s) 16, 54, 166, 508, 739, 1099, 1186, 1213
Bsp19I CCATGG 4 cut(s) 496, 967, 1005, 1248
BspACI CCGC 3 cut(s) 944, 1107, 1143
BspANI GGCC 4 cut(s) 340, 533, 1004, 1247
BspLI GGNNCC 5 cut(s) 18, 510, 579, 925, 942
BspMAI CTGCAG 1 cut(s) 210
BspMI ACCTGC 1 cut(s) 937
BspPI GGATC 5 cut(s) 11, 24, 161, 503, 516
BsrI ACTGG 2 cut(s) 507, 1287
BssECI CCNNGG 4 cut(s) 496, 967, 1005, 1248
BssMI GATC 8 cut(s) 16, 54, 166, 508, 739, 1099, 1186, 1213
BssNAI GTATAC 1 cut(s) 1118
BssT1I CCWWGG 4 cut(s) 496, 967, 1005, 1248
Bst1107I GTATAC 1 cut(s) 1118
Bst2UI CCWGG 4 cut(s) 83, 242, 641, 1194
Bst4CI ACNGT 2 cut(s) 433, 1231
Bst6I CTCTTC 2 cut(s) 583, 1054
BstAPI GCANNNNNTGC 2 cut(s) 205, 485
BstC8I GCNNGC 3 cut(s) 961, 1034, 1245
BstDEI CTNAG 1 cut(s) 973
BstDSI CCRYGG 4 cut(s) 496, 967, 1005, 1248
BstF5I GGATG 3 cut(s) 333, 1015, 1258
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 8 cut(s) 19, 57, 169, 511, 742, 1102, 1189, 1216
BstMAI GTCTC 3 cut(s) 214, 289, 1217
BstMBI GATC 8 cut(s) 16, 54, 166, 508, 739, 1099, 1186, 1213
BstMWI GCNNNNNNNGC 4 cut(s) 205, 485, 539, 969
BstNI CCWGG 4 cut(s) 83, 242, 641, 1194
BstNSI RCATGY 1 cut(s) 1027
BstSCI CCNGG 5 cut(s) 81, 240, 350, 639, 1192
BstSFI CTRYAG 1 cut(s) 206
BstV1I GCAGC 3 cut(s) 220, 432, 712
BstV2I GAAGAC 1 cut(s) 1140
BstX2I RGATCY 4 cut(s) 16, 508, 1099, 1186
BstYI RGATCY 4 cut(s) 16, 508, 1099, 1186
BstZ17I GTATAC 1 cut(s) 1118
BsuI GTATCC 1 cut(s) 889
BsuRI GGCC 4 cut(s) 340, 533, 1004, 1247
BtgI CCRYGG 4 cut(s) 496, 967, 1005, 1248
BtsCI GGATG 3 cut(s) 333, 1015, 1258
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 2 cut(s) 453, 1280
BveI ACCTGC 1 cut(s) 937
Cac8I GCNNGC 3 cut(s) 961, 1034, 1245
CaiI CAGNNNCTG 1 cut(s) 205
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 2 cut(s) 924, 940
Csp6I GTAC 2 cut(s) 139, 1082
CviJI RGCY 9 cut(s) 28, 340, 533, 565, 578, 972, 1004, 1036, 1247
CviKI_1 RGCY 9 cut(s) 28, 340, 533, 565, 578, 972, 1004, 1036, 1247
CviQI GTAC 2 cut(s) 139, 1082
DdeI CTNAG 1 cut(s) 973
DpnI GATC 8 cut(s) 18, 56, 168, 510, 741, 1101, 1188, 1215
DpnII GATC 8 cut(s) 16, 54, 166, 508, 739, 1099, 1186, 1213
Eam1104I CTCTTC 2 cut(s) 583, 1054
EarI CTCTTC 2 cut(s) 583, 1054
Eco130I CCWWGG 4 cut(s) 496, 967, 1005, 1248
Eco147I AGGCCT 1 cut(s) 340
Eco32I GATATC 2 cut(s) 1015, 1258
Eco47I GGWCC 2 cut(s) 924, 940
Eco57I CTGAAG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 752
EcoO109I RGGNCCY 1 cut(s) 940
EcoRII CCWGG 4 cut(s) 81, 240, 639, 1192
EcoRV GATATC 2 cut(s) 1015, 1258
EcoT14I CCWWGG 4 cut(s) 496, 967, 1005, 1248
ErhI CCWWGG 4 cut(s) 496, 967, 1005, 1248
FaqI GGGAC 2 cut(s) 73, 937
FauI CCCGC 2 cut(s) 951, 1100
FauNDI CATATG 1 cut(s) 193
FbaI TGATCA 1 cut(s) 739
FblI GTMKAC 1 cut(s) 1117
Fnu4HI GCNGC 3 cut(s) 209, 421, 701
FokI GGATG 3 cut(s) 340, 1022, 1265
Fsp4HI GCNGC 3 cut(s) 209, 421, 701
FspBI CTAG 3 cut(s) 342, 366, 876
GlaI GCGC 1 cut(s) 212
GluI GCNGC 3 cut(s) 209, 421, 701
GsuI CTGGAG 1 cut(s) 411
HaeIII GGCC 4 cut(s) 340, 533, 1004, 1247
HapII CCGG 2 cut(s) 352, 581
HhaI GCGC 1 cut(s) 213
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HinfI GANTC 5 cut(s) 49, 282, 636, 785, 1208
HpaII CCGG 2 cut(s) 352, 581
HphI GGTGA 4 cut(s) 140, 485, 875, 1157
Hpy166II GTNNAC 1 cut(s) 1118
Hpy188III TCNNGA 6 cut(s) 58, 288, 560, 743, 876, 1124
Hpy8I GTNNAC 1 cut(s) 1118
Hpy99I CGWCG 1 cut(s) 428
HpyAV CCTTC 2 cut(s) 860, 1062
HpyCH4III ACNGT 2 cut(s) 433, 1231
HpyCH4V TGCA 6 cut(s) 154, 208, 324, 420, 700, 1174
HpyF10VI GCNNNNNNNGC 4 cut(s) 205, 485, 539, 969
HpyF3I CTNAG 1 cut(s) 973
HspAI GCGC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 739
Kzo9I GATC 8 cut(s) 16, 54, 166, 508, 739, 1099, 1186, 1213
LmnI GCTCC 1 cut(s) 583
Lsp1109I GCAGC 3 cut(s) 220, 432, 712
LweI GCATC 2 cut(s) 385, 475
MaeI CTAG 3 cut(s) 342, 366, 876
MaeIII GTNAC 3 cut(s) 382, 491, 594
MalI GATC 8 cut(s) 18, 56, 168, 510, 741, 1101, 1188, 1215
MboI GATC 8 cut(s) 16, 54, 166, 508, 739, 1099, 1186, 1213
MboII GAAGA 9 cut(s) 118, 188, 494, 600, 780, 794, 1071, 1102, 1145
MflI RGATCY 4 cut(s) 16, 508, 1099, 1186
MluCI AATT 2 cut(s) 179, 291
MlyI GAGTC 1 cut(s) 291
MmeI TCCRAC 1 cut(s) 445
MroXI GAANNNNTTC 1 cut(s) 725
MseI TTAA 1 cut(s) 995
MslI CAYNNNNRTG 1 cut(s) 810
MspI CCGG 2 cut(s) 352, 581
MspR9I CCNGG 5 cut(s) 83, 242, 352, 641, 1194
Mva1269I GAATGC 2 cut(s) 326, 1281
MvaI CCWGG 4 cut(s) 83, 242, 641, 1194
MwoI GCNNNNNNNGC 4 cut(s) 205, 485, 539, 969
NciI CCSGG 1 cut(s) 352
NcoI CCATGG 4 cut(s) 496, 967, 1005, 1248
NdeI CATATG 1 cut(s) 193
NdeII GATC 8 cut(s) 16, 54, 166, 508, 739, 1099, 1186, 1213
NlaIV GGNNCC 5 cut(s) 18, 510, 579, 925, 942
NmuCI GTSAC 3 cut(s) 382, 491, 594
NspI RCATGY 1 cut(s) 1027
PaeR7I CTCGAG 1 cut(s) 752
PaqCI CACCTGC 1 cut(s) 937
PceI AGGCCT 1 cut(s) 340
PciI ACATGT 1 cut(s) 1023
PcsI WCGNNNNNNNCGW 1 cut(s) 44
PctI GAATGC 2 cut(s) 326, 1281
PdmI GAANNNNTTC 1 cut(s) 725
PfeI GAWTC 4 cut(s) 49, 636, 785, 1208
PflMI CCANNNNNTGG 1 cut(s) 502
PkrI GCNGC 3 cut(s) 210, 422, 702
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
PpuMI RGGWCCY 1 cut(s) 940
PscI ACATGT 1 cut(s) 1023
Psp5II RGGWCCY 1 cut(s) 940
Psp6I CCWGG 4 cut(s) 81, 240, 639, 1192
PspGI CCWGG 4 cut(s) 81, 240, 639, 1192
PspN4I GGNNCC 5 cut(s) 18, 510, 579, 925, 942
PspPI GGNCC 2 cut(s) 924, 940
PspPPI RGGWCCY 1 cut(s) 940
PspXI VCTCGAGB 1 cut(s) 752
PstI CTGCAG 1 cut(s) 210
PstNI CAGNNNCTG 1 cut(s) 205
PsuI RGATCY 4 cut(s) 16, 508, 1099, 1186
RsaI GTAC 2 cut(s) 140, 1083
RsaNI GTAC 2 cut(s) 139, 1082
RseI CAYNNNNRTG 1 cut(s) 810
SaqAI TTAA 1 cut(s) 995
SatI GCNGC 3 cut(s) 209, 421, 701
Sau3AI GATC 8 cut(s) 16, 54, 166, 508, 739, 1099, 1186, 1213
Sau96I GGNCC 2 cut(s) 924, 940
SchI GAGTC 1 cut(s) 291
ScrFI CCNGG 5 cut(s) 83, 242, 352, 641, 1194
SetI ASST 8 cut(s) 30, 119, 645, 694, 718, 871, 951, 1195
SfaNI GCATC 2 cut(s) 385, 475
SfcI CTRYAG 1 cut(s) 206
Sfr274I CTCGAG 1 cut(s) 752
SinI GGWCC 2 cut(s) 924, 940
SlaI CTCGAG 1 cut(s) 752
SmiMI CAYNNNNRTG 1 cut(s) 810
SmlI CTYRAG 1 cut(s) 752
SmoI CTYRAG 1 cut(s) 752
Sse9I AATT 2 cut(s) 179, 291
SseBI AGGCCT 1 cut(s) 340
SsiI CCGC 3 cut(s) 944, 1107, 1143
SspMI CTAG 3 cut(s) 342, 366, 876
StuI AGGCCT 1 cut(s) 340
StyD4I CCNGG 5 cut(s) 81, 240, 350, 639, 1192
StyI CCWWGG 4 cut(s) 496, 967, 1005, 1248
TaaI ACNGT 2 cut(s) 433, 1231
TaqI TCGA 3 cut(s) 289, 559, 753
TasI AATT 2 cut(s) 179, 291
TfiI GAWTC 4 cut(s) 49, 636, 785, 1208
Tru1I TTAA 1 cut(s) 995
Tru9I TTAA 1 cut(s) 995
TscAI CASTG 2 cut(s) 460, 1287
TseFI GTSAC 3 cut(s) 382, 491, 594
TseI GCWGC 3 cut(s) 208, 420, 700
Tsp45I GTSAC 3 cut(s) 382, 491, 594
TspDTI ATGAA 8 cut(s) 420, 539, 669, 718, 822, 861, 1146, 1196
TspGWI ACGGA 1 cut(s) 424
TspRI CASTG 2 cut(s) 460, 1287
Van91I CCANNNNNTGG 1 cut(s) 502
VpaK11BI GGWCC 2 cut(s) 924, 940
XbaI TCTAGA 1 cut(s) 875
XceI RCATGY 1 cut(s) 1027
XhoI CTCGAG 1 cut(s) 752
XmiI GTMKAC 1 cut(s) 1117
XmnI GAANNNNTTC 1 cut(s) 725
XspI CTAG 3 cut(s) 342, 366, 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.