Rh1CG346300

Mad3/BUB1 hoMad3/BUB1 homology region 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0010450
Physical Location & Seq
Forward (+)
62354199 .. 62357114
2916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG346300.1

Sequence Viewer

Length: 1335 bp
ATGGATTTTGTGGAGGATCCGAGAGAGCTTTTGAGGACGATGGAGGCGGATCGGATTGGGACGAAGCACCCTCTTTTCTACCAGGCATATGCTCTTTACTATGAGAAGAACAAGAAATTTGAGGAGGCTGAGAAGATGTACCATTTGGGGGTGCAGAACCATGCAGATCCCATTGAAGAACTAGAGAAAGCATATGAGCAGTTCCTGCAGCGCATGGAGAGACACAGGAAACGGAAACACCAGGAAAAGAGAACCACAAAAAGAGTAACAGATCGGAGTGGAGTCTCTCGTATTAATAGAGAAAGGAAAAATGAAAAGGAATGCAGGATCGGTGATAGGCCTACAGAAGCCCGGCATGATGGACCTAGAAGTAAGCATCAAGTGACTGCTGGAGGAGGGTCAGATGAACGGATGTTGCCTTGCAGCGATGATACAGTCGTGGGGAAGTTTGTAGACACTGCCATTGTCGGAAAATCTGAAGCAGAAGATGCTTGTCACCATGGACTGGTGGATCCTACTATAAACATGAAAGAGGCCATGAGTGCCATAAATAGTATGTTTCGAGAGCCTTTAGAGATGACTCCAGTTAGAAGAGGATCACGCCAATGCCAACAAAAGGAAAATAACACAAACAATGGATTCCAGGTGTTTGTTGATGAAAACTTGGATAAGGGAGGCAAATCAAAAGATAAAGGTGTTTCAATGGTGCAGCAAAGTAGAACTGCTACCCATCAGTCTCACCGAGAACCTTTAAACATTTTCATTGATGATGATCAAGATAGCCTTGATCAGAGTGAAGCTCAGAATATGACAGAAGATTCTTCCCCATCTGCTTCACATGGAAATGCTTTTGTGTTTCTGAATCCAAAAGATGTTTCAAGTGAAAGTCCTAATGGTTTGGATGTCGAAGGTTCATCTAGACCAAGGTTTAGAGAGGATACGATGATGGTTCGTAGGATTGTTGGGACCACCATTTCAGAGGACCCGCAGGTGGAAAACGCTTGCCACCATGGCTTAGTTGAACCAACTATCAACTTAAAGGAGGCCATGGATGATATCAATAACATGTTTGGCAAGCCAATAGATTTTGTTAGAAGCAGAAGAGTTAAAAGGCAGGACAAAGCACCAGAGAAGAAACAAGATCTTGGTGGGTTTTGTATACTTCCTGATGATGAAGATGACGATGACGATGATAACTTGGAACAACAACAAGTGCAGAAAATGAAAGATCTACCGGGAAAATCAAAAGATTCTGATCTGTTTGAGACTACTGTTTTTACAAAGCAGGCCATGGATGATATCAATAAGATGTTTGGAATGCCACTGGACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

444

Amino Acids

50.81

Weight (kDa)

5.4

Isoelectric Point (pI)

53.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 1 - 51 1.5e-11 Mad3/BUB1 homology region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05510
fragaria_vesca FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900
malus_domestica MD01G1156700.v1.1 MD07G1225400.v1.1
prunus_persica Prupe.2G254200_v2.0.a1
pyrus_communis pycom01g17350 pycom07g20410
rosa_chinensis RchiOBHm_Chr1g0370781
rosa_laevigata RLG00000027010
rosa_multiflora Rmu_sc0009474.1_g000004
rosa_roxburghii Rroxscaffold_4G00286230
rosa_rugosa Rorug01G0359700
rosa_samantha Rh1AG368800 Rh1BG332400 Rh1CG346300 Rh1DG363800
rosa_wichuraiana Rw1G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 979
Acc36I ACCTGC 1 cut(s) 979
AccB7I CCANNNNNTGG 1 cut(s) 505
AccI GTMKAC 2 cut(s) 453, 1159
AciI CCGC 2 cut(s) 47, 986
AclWI GGATC 8 cut(s) 11, 24, 57, 161, 335, 506, 519, 604
AcsI RAATTY 1 cut(s) 116
AcuI CTGAAG 1 cut(s) 498
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 4 cut(s) 148, 505, 616, 991
AflIII ACRYGT 1 cut(s) 1065
AgsI TTSAA 4 cut(s) 176, 702, 879, 1022
AjnI CCWGG 3 cut(s) 81, 240, 642
AjuI GAANNNNNNNTTGG 2 cut(s) 859, 891
AluBI AGCT 2 cut(s) 28, 800
AluI AGCT 2 cut(s) 28, 800
Alw26I GTCTC 4 cut(s) 214, 289, 741, 1259
AlwI GGATC 8 cut(s) 11, 24, 57, 161, 335, 506, 519, 604
AlwNI CAGNNNCTG 1 cut(s) 205
AoxI GGCC 4 cut(s) 338, 534, 1044, 1287
ApeKI GCWGC 3 cut(s) 208, 423, 709
ApoI RAATTY 1 cut(s) 116
AseI ATTAAT 1 cut(s) 294
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 3 cut(s) 362, 966, 982
AsuC2I CCSGG 2 cut(s) 352, 1236
AsuHPI GGTGA 3 cut(s) 344, 488, 731
AvaII GGWCC 3 cut(s) 362, 966, 982
BaeI ACNNNNGTAYC 2 cut(s) 423, 456
BamHI GGATCC 2 cut(s) 16, 511
BbvI GCAGC 3 cut(s) 220, 435, 721
BccI CCATC 5 cut(s) 34, 353, 738, 835, 940
BciT130I CCWGG 3 cut(s) 83, 242, 644
BciVI GTATCC 1 cut(s) 931
BclI TGATCA 2 cut(s) 772, 787
BcnI CCSGG 2 cut(s) 352, 1236
BcoDI GTCTC 4 cut(s) 214, 289, 741, 1259
BfaI CTAG 3 cut(s) 182, 366, 918
BfmI CTRYAG 2 cut(s) 206, 342
BfuAI ACCTGC 1 cut(s) 979
BfuI GTATCC 1 cut(s) 931
BglI GCCNNNNNGGC 1 cut(s) 1011
BglII AGATCT 2 cut(s) 1141, 1228
BisI GCNGC 3 cut(s) 209, 424, 710
BlsI GCNGC 3 cut(s) 210, 425, 711
Bme1390I CCNGG 5 cut(s) 83, 242, 352, 644, 1236
Bme18I GGWCC 3 cut(s) 362, 966, 982
BmgT120I GGNCC 3 cut(s) 362, 966, 982
BmiI GGNNCC 4 cut(s) 18, 513, 967, 984
BmrFI CCNGG 5 cut(s) 83, 242, 352, 644, 1236
BmsI GCATC 2 cut(s) 385, 478
BplI GAGNNNNNCTC 2 cut(s) 784, 816
BpmI CTGGAG 2 cut(s) 411, 567
BpuMI CCSGG 2 cut(s) 352, 1236
BsaBI GATNNNNATC 2 cut(s) 771, 1254
BsaJI CCNNGG 5 cut(s) 499, 923, 1009, 1047, 1290
Bsc4I CCNNNNNNNGG 4 cut(s) 148, 505, 616, 991
Bse1I ACTGG 3 cut(s) 510, 584, 1329
Bse8I GATNNNNATC 2 cut(s) 771, 1254
BseBI CCWGG 3 cut(s) 83, 242, 644
BseDI CCNNGG 5 cut(s) 499, 923, 1009, 1047, 1290
BseGI GGATG 4 cut(s) 417, 907, 1057, 1300
BseJI GATNNNNATC 2 cut(s) 771, 1254
BseLI CCNNNNNNNGG 4 cut(s) 148, 505, 616, 991
BseMII CTCAG 2 cut(s) 120, 815
BseNI ACTGG 3 cut(s) 510, 584, 1329
BseRI GAGGAG 2 cut(s) 137, 408
BseXI GCAGC 3 cut(s) 220, 435, 721
BsgI GTGCAG 3 cut(s) 173, 728, 1235
BshFI GGCC 4 cut(s) 340, 536, 1046, 1289
BsiSI CCGG 2 cut(s) 352, 1235
BslFI GGGAC 2 cut(s) 73, 979
BslI CCNNNNNNNGG 4 cut(s) 148, 505, 616, 991
BsmAI GTCTC 4 cut(s) 214, 289, 741, 1259
BsmFI GGGAC 2 cut(s) 73, 979
BsmI GAATGC 2 cut(s) 326, 1323
BsnI GGCC 4 cut(s) 340, 536, 1046, 1289
Bsp19I CCATGG 4 cut(s) 499, 1009, 1047, 1290
BspACI CCGC 2 cut(s) 47, 986
BspANI GGCC 4 cut(s) 340, 536, 1046, 1289
BspCNI CTCAG 2 cut(s) 121, 814
BspLI GGNNCC 4 cut(s) 18, 513, 967, 984
BspMAI CTGCAG 1 cut(s) 210
BspMI ACCTGC 1 cut(s) 979
BspPI GGATC 8 cut(s) 11, 24, 57, 161, 335, 506, 519, 604
BsrI ACTGG 3 cut(s) 510, 584, 1329
BssECI CCNNGG 5 cut(s) 499, 923, 1009, 1047, 1290
BssNAI GTATAC 1 cut(s) 1160
BssT1I CCWWGG 5 cut(s) 499, 923, 1009, 1047, 1290
Bst1107I GTATAC 1 cut(s) 1160
Bst2UI CCWGG 3 cut(s) 83, 242, 644
Bst4CI ACNGT 2 cut(s) 436, 1273
Bst6I CTCTTC 2 cut(s) 586, 1096
BstAPI GCANNNNNTGC 2 cut(s) 205, 488
BstC8I GCNNGC 3 cut(s) 1003, 1076, 1287
BstDEI CTNAG 3 cut(s) 129, 801, 1015
BstDSI CCRYGG 4 cut(s) 499, 1009, 1047, 1290
BstF5I GGATG 4 cut(s) 417, 907, 1057, 1300
BstHHI GCGC 1 cut(s) 213
BstMAI GTCTC 4 cut(s) 214, 289, 741, 1259
BstMWI GCNNNNNNNGC 4 cut(s) 205, 488, 542, 1011
BstNI CCWGG 3 cut(s) 83, 242, 644
BstNSI RCATGY 1 cut(s) 1069
BstSCI CCNGG 5 cut(s) 81, 240, 350, 642, 1234
BstSFI CTRYAG 2 cut(s) 206, 342
BstV1I GCAGC 3 cut(s) 220, 435, 721
BstX2I RGATCY 5 cut(s) 16, 166, 511, 1141, 1228
BstYI RGATCY 5 cut(s) 16, 166, 511, 1141, 1228
BstZ17I GTATAC 1 cut(s) 1160
BsuI GTATCC 1 cut(s) 931
BsuRI GGCC 4 cut(s) 340, 536, 1046, 1289
BtgI CCRYGG 4 cut(s) 499, 1009, 1047, 1290
BtgZI GCGATG 1 cut(s) 441
BtsCI GGATG 4 cut(s) 417, 907, 1057, 1300
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 2 cut(s) 456, 1322
BveI ACCTGC 1 cut(s) 979
Cac8I GCNNGC 3 cut(s) 1003, 1076, 1287
CaiI CAGNNNCTG 1 cut(s) 205
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 3 cut(s) 362, 966, 982
Csp6I GTAC 1 cut(s) 139
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 3 cut(s) 129, 801, 1015
DraI TTTAAA 1 cut(s) 753
Eam1104I CTCTTC 2 cut(s) 586, 1096
EarI CTCTTC 2 cut(s) 586, 1096
EciI GGCGGA 1 cut(s) 62
Eco130I CCWWGG 5 cut(s) 499, 923, 1009, 1047, 1290
Eco147I AGGCCT 1 cut(s) 340
Eco32I GATATC 2 cut(s) 1057, 1300
Eco47I GGWCC 3 cut(s) 362, 966, 982
Eco57I CTGAAG 1 cut(s) 498
EcoO109I RGGNCCY 1 cut(s) 982
EcoRII CCWGG 3 cut(s) 81, 240, 642
EcoRV GATATC 2 cut(s) 1057, 1300
EcoT14I CCWWGG 5 cut(s) 499, 923, 1009, 1047, 1290
ErhI CCWWGG 5 cut(s) 499, 923, 1009, 1047, 1290
FalI AAGNNNNNCTT 2 cut(s) 768, 800
FaqI GGGAC 2 cut(s) 73, 979
FauI CCCGC 1 cut(s) 993
FauNDI CATATG 2 cut(s) 88, 193
FbaI TGATCA 2 cut(s) 772, 787
FblI GTMKAC 2 cut(s) 453, 1159
Fnu4HI GCNGC 3 cut(s) 209, 424, 710
FokI GGATG 4 cut(s) 424, 914, 1064, 1307
Fsp4HI GCNGC 3 cut(s) 209, 424, 710
FspBI CTAG 3 cut(s) 182, 366, 918
GlaI GCGC 1 cut(s) 212
GluI GCNGC 3 cut(s) 209, 424, 710
GsuI CTGGAG 2 cut(s) 411, 567
HaeIII GGCC 4 cut(s) 340, 536, 1046, 1289
HapII CCGG 2 cut(s) 352, 1235
HhaI GCGC 1 cut(s) 213
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HinfI GANTC 6 cut(s) 282, 580, 639, 818, 862, 1250
HpaII CCGG 2 cut(s) 352, 1235
HphI GGTGA 3 cut(s) 344, 488, 731
Hpy166II GTNNAC 2 cut(s) 454, 1160
Hpy188III TCNNGA 4 cut(s) 563, 776, 918, 1166
Hpy8I GTNNAC 2 cut(s) 454, 1160
HpyAV CCTTC 1 cut(s) 902
HpyCH4III ACNGT 2 cut(s) 436, 1273
HpyCH4V TGCA 7 cut(s) 154, 164, 208, 324, 423, 709, 1216
HpyF10VI GCNNNNNNNGC 4 cut(s) 205, 488, 542, 1011
HpyF3I CTNAG 3 cut(s) 129, 801, 1015
HspAI GCGC 1 cut(s) 211
Ksp22I TGATCA 2 cut(s) 772, 787
Lsp1109I GCAGC 3 cut(s) 220, 435, 721
LweI GCATC 2 cut(s) 385, 478
MaeI CTAG 3 cut(s) 182, 366, 918
MaeIII GTNAC 3 cut(s) 265, 382, 494
MflI RGATCY 5 cut(s) 16, 166, 511, 1141, 1228
MluCI AATT 1 cut(s) 116
MlyI GAGTC 2 cut(s) 291, 574
MmeI TCCRAC 1 cut(s) 448
MseI TTAA 4 cut(s) 294, 752, 1037, 1107
MslI CAYNNNNRTG 2 cut(s) 604, 843
MspI CCGG 2 cut(s) 352, 1235
MspR9I CCNGG 5 cut(s) 83, 242, 352, 644, 1236
Mva1269I GAATGC 2 cut(s) 326, 1323
MvaI CCWGG 3 cut(s) 83, 242, 644
MwoI GCNNNNNNNGC 4 cut(s) 205, 488, 542, 1011
NciI CCSGG 2 cut(s) 352, 1236
NcoI CCATGG 4 cut(s) 499, 1009, 1047, 1290
NdeI CATATG 2 cut(s) 88, 193
NlaIV GGNNCC 4 cut(s) 18, 513, 967, 984
NmuCI GTSAC 2 cut(s) 382, 494
NspI RCATGY 1 cut(s) 1069
PaqCI CACCTGC 1 cut(s) 979
PceI AGGCCT 1 cut(s) 340
PciI ACATGT 1 cut(s) 1065
PctI GAATGC 2 cut(s) 326, 1323
PfeI GAWTC 4 cut(s) 639, 818, 862, 1250
PflMI CCANNNNNTGG 1 cut(s) 505
PkrI GCNGC 3 cut(s) 210, 425, 711
PleI GAGTC 2 cut(s) 290, 574
PpsI GAGTC 2 cut(s) 290, 574
PpuMI RGGWCCY 1 cut(s) 982
PscI ACATGT 1 cut(s) 1065
PshBI ATTAAT 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 982
Psp6I CCWGG 3 cut(s) 81, 240, 642
PspGI CCWGG 3 cut(s) 81, 240, 642
PspN4I GGNNCC 4 cut(s) 18, 513, 967, 984
PspPI GGNCC 3 cut(s) 362, 966, 982
PspPPI RGGWCCY 1 cut(s) 982
PstI CTGCAG 1 cut(s) 210
PstNI CAGNNNCTG 1 cut(s) 205
PsuI RGATCY 5 cut(s) 16, 166, 511, 1141, 1228
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 2 cut(s) 604, 843
SaqAI TTAA 4 cut(s) 294, 752, 1037, 1107
SatI GCNGC 3 cut(s) 209, 424, 710
Sau96I GGNCC 3 cut(s) 362, 966, 982
SchI GAGTC 2 cut(s) 291, 574
ScrFI CCNGG 5 cut(s) 83, 242, 352, 644, 1236
SetI ASST 9 cut(s) 30, 367, 648, 697, 751, 802, 913, 929, 993
SfaNI GCATC 2 cut(s) 385, 478
SfcI CTRYAG 2 cut(s) 206, 342
SinI GGWCC 3 cut(s) 362, 966, 982
SmiMI CAYNNNNRTG 2 cut(s) 604, 843
Sse9I AATT 1 cut(s) 116
SseBI AGGCCT 1 cut(s) 340
SsiI CCGC 2 cut(s) 47, 986
SspMI CTAG 3 cut(s) 182, 366, 918
StuI AGGCCT 1 cut(s) 340
StyD4I CCNGG 5 cut(s) 81, 240, 350, 642, 1234
StyI CCWWGG 5 cut(s) 499, 923, 1009, 1047, 1290
TaaI ACNGT 2 cut(s) 436, 1273
TaqI TCGA 2 cut(s) 562, 906
TasI AATT 1 cut(s) 116
TfiI GAWTC 4 cut(s) 639, 818, 862, 1250
Tru1I TTAA 4 cut(s) 294, 752, 1037, 1107
Tru9I TTAA 4 cut(s) 294, 752, 1037, 1107
TscAI CASTG 2 cut(s) 463, 1329
TseFI GTSAC 2 cut(s) 382, 494
TseI GCWGC 3 cut(s) 208, 423, 709
Tsp45I GTSAC 2 cut(s) 382, 494
TspDTI ATGAA 8 cut(s) 327, 420, 542, 672, 751, 903, 1188, 1238
TspGWI ACGGA 2 cut(s) 247, 424
TspRI CASTG 2 cut(s) 463, 1329
Van91I CCANNNNNTGG 1 cut(s) 505
VpaK11BI GGWCC 3 cut(s) 362, 966, 982
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 1 cut(s) 116
XbaI TCTAGA 1 cut(s) 917
XceI RCATGY 1 cut(s) 1069
XmiI GTMKAC 2 cut(s) 453, 1159
XspI CTAG 3 cut(s) 182, 366, 918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.