pycom01g17350

Mad3/BUB1 hoMad3/BUB1 homology region 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0010450
Physical Location & Seq
Forward (+)
16673212 .. 16674635
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g17350.1

Sequence Viewer

Length: 816 bp
ATGTCCAACAACGATCTTCTCACCTCCTTAATCTCCGACATCAAAACCTACTCCGGCAAAGACCCTCTTCTTCCATGGCTAAGAGGGATTCGAAAAATGAACGACTCACTCCCACCTCGCACTCTGAAGGAGAAGCTCCCTCGCTTCTTGCAGAAATGCGCGCAGACATTCGAGTCCGATCGGCGATACCGAAACGACCTGCGCTACATTAGAGTTTGGTTAAAGTTGATGGATTTTGTCGATGATCCAAGAGCTCTGTTGAGAACAATGGAGGCGAATCAAATCGGGACAAAGCACTCCTTGTTCTACCAGGCATATGCTCTCTACTATGAAAAGACCAAGAAATTTGAGGAGGCTGAGAAGATGTACCACTTGGGAGTGCAAAACCTTGCAGAGCCCATTGGAGCGTTAGAGAAGTCGTACGAGCAGTTCCTTCAACGCATGGAGAGACATAGGAGACACAGAAACCAGGAAAAGAGGACCGTGAAAAGGCCTTTATCGGATCCGACTATCCCTCCAAATGGAGAAAGAAACAAAGAAAATGCCTGCAAAATCGGAGCCAGGCCTATAGAATCCCAACATGATCAGTCTAGAAGTAAGCGTCAAGTGACTTCTGGAAGAGGGTCAGAGGAACCGGAGATGTTTGCCGGCGATGATACGGTTGTGGTGAAGTTTGTAGACACTGCCATTGTTGGAATGTCTGAAGCGGAAGATGCGTGTCATCATGGATTGGTGGATCCATCCTGTAAACATGAAAGAGGCCATGAGCGCCATCAACGACATGTTTCGAGAGCCTATAGAGACGGCTCCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

272

Amino Acids

31.65

Weight (kDa)

9.33

Isoelectric Point (pI)

52.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 10 - 127 1.8e-37 Mad3/BUB1 homology region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05510
fragaria_vesca FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900
malus_domestica MD01G1156700.v1.1 MD07G1225400.v1.1
prunus_persica Prupe.2G254200_v2.0.a1
pyrus_communis pycom01g17350 pycom07g20410
rosa_chinensis RchiOBHm_Chr1g0370781
rosa_laevigata RLG00000027010
rosa_multiflora Rmu_sc0009474.1_g000004
rosa_roxburghii Rroxscaffold_4G00286230
rosa_rugosa Rorug01G0359700
rosa_samantha Rh1AG368800 Rh1BG332400 Rh1CG346300 Rh1DG363800
rosa_wichuraiana Rw1G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 172
Acc36I ACCTGC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 678
AccII CGCG 1 cut(s) 161
AciI CCGC 1 cut(s) 707
AclWI GGATC 5 cut(s) 239, 497, 510, 731, 744
AcsI RAATTY 1 cut(s) 344
AcuI CTGAAG 2 cut(s) 146, 723
AfaI GTAC 2 cut(s) 368, 422
AfiI CCNNNNNNNGG 2 cut(s) 489, 521
AflIII ACRYGT 1 cut(s) 781
AgsI TTSAA 1 cut(s) 437
AjnI CCWGG 3 cut(s) 309, 468, 560
AluBI AGCT 2 cut(s) 136, 254
AluI AGCT 2 cut(s) 136, 254
Alw21I GWGCWC 1 cut(s) 256
Alw26I GTCTC 3 cut(s) 442, 451, 795
AlwI GGATC 5 cut(s) 239, 497, 510, 731, 744
AoxI GGCC 3 cut(s) 491, 563, 760
ApoI RAATTY 1 cut(s) 344
AspLEI GCGC 4 cut(s) 161, 163, 204, 771
AspS9I GGNCC 1 cut(s) 480
AsuHPI GGTGA 2 cut(s) 13, 679
AsuII TTCGAA 1 cut(s) 91
AvaII GGWCC 1 cut(s) 480
BaeI ACNNNNGTAYC 2 cut(s) 648, 681
BamHI GGATCC 2 cut(s) 502, 736
BanII GRGCYC 2 cut(s) 256, 399
Bbv12I GWGCWC 1 cut(s) 256
BccI CCATC 3 cut(s) 223, 748, 780
BciT130I CCWGG 3 cut(s) 311, 470, 562
BclI TGATCA 1 cut(s) 583
BcoDI GTCTC 3 cut(s) 442, 451, 795
BfaI CTAG 1 cut(s) 591
BfmI CTRYAG 2 cut(s) 567, 796
BfoI RGCGCY 1 cut(s) 772
BfuAI ACCTGC 1 cut(s) 207
Bme1390I CCNGG 3 cut(s) 311, 470, 562
Bme18I GGWCC 1 cut(s) 480
BmgT120I GGNCC 1 cut(s) 480
BmiI GGNNCC 5 cut(s) 504, 559, 633, 738, 808
BmrFI CCNGG 3 cut(s) 311, 470, 562
BmsI GCATC 1 cut(s) 703
Bpu14I TTCGAA 1 cut(s) 91
BsaJI CCNNGG 1 cut(s) 74
BsaWI WCCGGW 1 cut(s) 634
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bsc4I CCNNNNNNNGG 2 cut(s) 489, 521
Bse118I RCCGGY 1 cut(s) 647
BseBI CCWGG 3 cut(s) 311, 470, 562
BseDI CCNNGG 1 cut(s) 74
BseGI GGATG 1 cut(s) 740
BseLI CCNNNNNNNGG 2 cut(s) 489, 521
BseMII CTCAG 1 cut(s) 348
BsePI GCGCGC 1 cut(s) 159
BseRI GAGGAG 1 cut(s) 365
Bsh1236I CGCG 1 cut(s) 161
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 3 cut(s) 493, 565, 762
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 256
BsiSI CCGG 3 cut(s) 54, 635, 648
BsiWI CGTACG 1 cut(s) 420
BslFI GGGAC 1 cut(s) 301
BslI CCNNNNNNNGG 2 cut(s) 489, 521
BsmAI GTCTC 3 cut(s) 442, 451, 795
BsmBI CGTCTC 1 cut(s) 795
BsmFI GGGAC 1 cut(s) 301
BsnI GGCC 3 cut(s) 493, 565, 762
Bsp119I TTCGAA 1 cut(s) 91
Bsp1286I GDGCHC 2 cut(s) 256, 399
Bsp143I GATC 6 cut(s) 13, 178, 244, 502, 583, 736
Bsp19I CCATGG 1 cut(s) 74
BspACI CCGC 1 cut(s) 707
BspANI GGCC 3 cut(s) 493, 565, 762
BspCNI CTCAG 1 cut(s) 349
BspFNI CGCG 1 cut(s) 161
BspLI GGNNCC 5 cut(s) 504, 559, 633, 738, 808
BspMI ACCTGC 1 cut(s) 207
BspPI GGATC 5 cut(s) 239, 497, 510, 731, 744
BspT104I TTCGAA 1 cut(s) 91
BsrFI RCCGGY 1 cut(s) 647
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 1 cut(s) 74
BssHII GCGCGC 1 cut(s) 159
BssMI GATC 6 cut(s) 13, 178, 244, 502, 583, 736
BssT1I CCWWGG 1 cut(s) 74
Bst2UI CCWGG 3 cut(s) 311, 470, 562
Bst4CI ACNGT 2 cut(s) 484, 661
Bst6I CTCTTC 2 cut(s) 72, 613
BstBI TTCGAA 1 cut(s) 91
BstC8I GCNNGC 3 cut(s) 161, 547, 649
BstDEI CTNAG 2 cut(s) 80, 357
BstDSI CCRYGG 1 cut(s) 74
BstF5I GGATG 1 cut(s) 740
BstFNI CGCG 1 cut(s) 161
BstH2I RGCGCY 1 cut(s) 772
BstHHI GCGC 4 cut(s) 161, 163, 204, 771
BstKTI GATC 6 cut(s) 16, 181, 247, 505, 586, 739
BstMAI GTCTC 3 cut(s) 442, 451, 795
BstMBI GATC 6 cut(s) 13, 178, 244, 502, 583, 736
BstMCI CGRYCG 1 cut(s) 181
BstMWI GCNNNNNNNGC 2 cut(s) 713, 768
BstNI CCWGG 3 cut(s) 311, 470, 562
BstNSI RCATGY 1 cut(s) 785
BstSCI CCNGG 3 cut(s) 309, 468, 560
BstSFI CTRYAG 2 cut(s) 567, 796
BstUI CGCG 1 cut(s) 161
BstX2I RGATCY 2 cut(s) 502, 736
BstYI RGATCY 2 cut(s) 502, 736
BsuRI GGCC 3 cut(s) 493, 565, 762
BtgI CCRYGG 1 cut(s) 74
BtgZI GCGATG 1 cut(s) 666
BtsCI GGATG 1 cut(s) 740
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 1 cut(s) 681
BveI ACCTGC 1 cut(s) 207
Cac8I GCNNGC 3 cut(s) 161, 547, 649
CfoI GCGC 4 cut(s) 161, 163, 204, 771
Cfr10I RCCGGY 1 cut(s) 647
Cfr13I GGNCC 1 cut(s) 480
CseI GACGC 1 cut(s) 590
Csp6I GTAC 2 cut(s) 367, 421
CviAII CATG 7 cut(s) 75, 442, 581, 725, 752, 764, 782
CviQI GTAC 2 cut(s) 367, 421
DdeI CTNAG 2 cut(s) 80, 357
DpnI GATC 6 cut(s) 15, 180, 246, 504, 585, 738
DpnII GATC 6 cut(s) 13, 178, 244, 502, 583, 736
DrdI GACNNNNNNGTC 1 cut(s) 172
DseDI GACNNNNNNGTC 1 cut(s) 172
Eam1104I CTCTTC 2 cut(s) 72, 613
EarI CTCTTC 2 cut(s) 72, 613
Ecl136II GAGCTC 1 cut(s) 254
Eco130I CCWWGG 1 cut(s) 74
Eco147I AGGCCT 2 cut(s) 493, 565
Eco24I GRGCYC 2 cut(s) 256, 399
Eco47I GGWCC 1 cut(s) 480
Eco53kI GAGCTC 1 cut(s) 254
Eco57I CTGAAG 2 cut(s) 146, 723
EcoICRI GAGCTC 1 cut(s) 254
EcoRII CCWGG 3 cut(s) 309, 468, 560
EcoT14I CCWWGG 1 cut(s) 74
EcoT38I GRGCYC 2 cut(s) 256, 399
ErhI CCWWGG 1 cut(s) 74
Esp3I CGTCTC 1 cut(s) 795
FaeI CATG 7 cut(s) 78, 445, 584, 728, 755, 767, 785
FalI AAGNNNNNCTT 4 cut(s) 51, 83, 284, 316
FaqI GGGAC 1 cut(s) 301
FatI CATG 7 cut(s) 74, 441, 580, 724, 751, 763, 781
FauNDI CATATG 1 cut(s) 316
FbaI TGATCA 1 cut(s) 583
FblI GTMKAC 1 cut(s) 678
FokI GGATG 1 cut(s) 727
FriOI GRGCYC 2 cut(s) 256, 399
FspBI CTAG 1 cut(s) 591
GlaI GCGC 4 cut(s) 160, 162, 203, 770
HaeII RGCGCY 1 cut(s) 772
HaeIII GGCC 3 cut(s) 493, 565, 762
HapII CCGG 3 cut(s) 54, 635, 648
HgaI GACGC 1 cut(s) 590
HhaI GCGC 4 cut(s) 161, 163, 204, 771
Hin1II CATG 7 cut(s) 78, 445, 584, 728, 755, 767, 785
Hin6I GCGC 4 cut(s) 159, 161, 202, 769
HinP1I GCGC 4 cut(s) 159, 161, 202, 769
HinfI GANTC 5 cut(s) 88, 104, 173, 277, 572
HpaII CCGG 3 cut(s) 54, 635, 648
HphI GGTGA 2 cut(s) 13, 679
Hpy166II GTNNAC 2 cut(s) 679, 749
Hpy188I TCNGA 8 cut(s) 37, 126, 178, 502, 507, 557, 628, 703
Hpy188III TCNNGA 4 cut(s) 286, 591, 615, 789
Hpy8I GTNNAC 2 cut(s) 679, 749
HpyAV CCTTC 2 cut(s) 121, 443
HpyCH4III ACNGT 2 cut(s) 484, 661
HpyCH4V TGCA 4 cut(s) 151, 382, 392, 549
HpyF10VI GCNNNNNNNGC 2 cut(s) 713, 768
HpyF3I CTNAG 2 cut(s) 80, 357
Hsp92II CATG 7 cut(s) 78, 445, 584, 728, 755, 767, 785
HspAI GCGC 4 cut(s) 159, 161, 202, 769
KroI GCCGGC 1 cut(s) 647
KroNI GCCGGC 1 cut(s) 649
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 6 cut(s) 13, 178, 244, 502, 583, 736
LmnI GCTCC 4 cut(s) 141, 404, 557, 812
LweI GCATC 1 cut(s) 703
MaeI CTAG 1 cut(s) 591
MaeIII GTNAC 1 cut(s) 607
MalI GATC 6 cut(s) 15, 180, 246, 504, 585, 738
MboI GATC 6 cut(s) 13, 178, 244, 502, 583, 736
MboII GAAGA 6 cut(s) 8, 59, 62, 373, 630, 722
MflI RGATCY 2 cut(s) 502, 736
MhlI GDGCHC 2 cut(s) 256, 399
MluCI AATT 1 cut(s) 344
MlyI GAGTC 2 cut(s) 98, 182
MmeI TCCRAC 4 cut(s) 30, 60, 530, 673
MroNI GCCGGC 1 cut(s) 647
MseI TTAA 2 cut(s) 29, 221
MspI CCGG 3 cut(s) 54, 635, 648
MspR9I CCNGG 3 cut(s) 311, 470, 562
MvaI CCWGG 3 cut(s) 311, 470, 562
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 2 cut(s) 713, 768
NaeI GCCGGC 1 cut(s) 649
NcoI CCATGG 1 cut(s) 74
NdeI CATATG 1 cut(s) 316
NdeII GATC 6 cut(s) 13, 178, 244, 502, 583, 736
NgoMIV GCCGGC 1 cut(s) 647
NlaIII CATG 7 cut(s) 78, 445, 584, 728, 755, 767, 785
NlaIV GGNNCC 5 cut(s) 504, 559, 633, 738, 808
NmuCI GTSAC 1 cut(s) 607
NspI RCATGY 1 cut(s) 785
NspV TTCGAA 1 cut(s) 91
PauI GCGCGC 1 cut(s) 159
PceI AGGCCT 2 cut(s) 493, 565
PciI ACATGT 1 cut(s) 781
PcsI WCGNNNNNNNCGW 1 cut(s) 187
PdiI GCCGGC 1 cut(s) 649
PfeI GAWTC 3 cut(s) 88, 277, 572
Pfl23II CGTACG 1 cut(s) 420
Ple19I CGATCG 1 cut(s) 181
PleI GAGTC 2 cut(s) 98, 181
PpsI GAGTC 2 cut(s) 98, 181
PscI ACATGT 1 cut(s) 781
Psp124BI GAGCTC 1 cut(s) 256
Psp6I CCWGG 3 cut(s) 309, 468, 560
PspGI CCWGG 3 cut(s) 309, 468, 560
PspLI CGTACG 1 cut(s) 420
PspN4I GGNNCC 5 cut(s) 504, 559, 633, 738, 808
PspPI GGNCC 1 cut(s) 480
PsrI GAACNNNNNNTAC 2 cut(s) 413, 445
PsuI RGATCY 2 cut(s) 502, 736
PteI GCGCGC 1 cut(s) 159
PvuI CGATCG 1 cut(s) 181
RsaI GTAC 2 cut(s) 368, 422
RsaNI GTAC 2 cut(s) 367, 421
SacI GAGCTC 1 cut(s) 256
SaqAI TTAA 2 cut(s) 29, 221
Sau3AI GATC 6 cut(s) 13, 178, 244, 502, 583, 736
Sau96I GGNCC 1 cut(s) 480
SchI GAGTC 2 cut(s) 98, 182
ScrFI CCNGG 3 cut(s) 311, 470, 562
SduI GDGCHC 2 cut(s) 256, 399
SetI ASST 7 cut(s) 26, 50, 118, 138, 201, 256, 390
SfaNI GCATC 1 cut(s) 703
SfcI CTRYAG 2 cut(s) 567, 796
SfuI TTCGAA 1 cut(s) 91
SinI GGWCC 1 cut(s) 480
Sse9I AATT 1 cut(s) 344
SseBI AGGCCT 2 cut(s) 493, 565
SsiI CCGC 1 cut(s) 707
SspMI CTAG 1 cut(s) 591
SstI GAGCTC 1 cut(s) 256
StuI AGGCCT 2 cut(s) 493, 565
StyD4I CCNGG 3 cut(s) 309, 468, 560
StyI CCWWGG 1 cut(s) 74
TaaI ACNGT 2 cut(s) 484, 661
TaqI TCGA 4 cut(s) 91, 171, 240, 788
TasI AATT 1 cut(s) 344
TfiI GAWTC 3 cut(s) 88, 277, 572
Tru1I TTAA 2 cut(s) 29, 221
Tru9I TTAA 2 cut(s) 29, 221
TscAI CASTG 1 cut(s) 688
TseFI GTSAC 1 cut(s) 607
Tsp45I GTSAC 1 cut(s) 607
TspDTI ATGAA 3 cut(s) 113, 345, 768
TspRI CASTG 1 cut(s) 688
VpaK11BI GGWCC 1 cut(s) 480
XapI RAATTY 1 cut(s) 344
XbaI TCTAGA 1 cut(s) 590
XceI RCATGY 1 cut(s) 785
XmiI GTMKAC 1 cut(s) 678
XspI CTAG 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.