Prupe.2G254200_v2.0.a1

Mad3/BUB1 hoMad3/BUB1 homology region 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0010450
Physical Location & Seq
Forward (+)
26719925 .. 26722670
2746 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G254200.1

Sequence Viewer

Length: 1548 bp
ATGGCCAACAACGATCTCTTCACATCGTTAATCGCAGAAATCAAAGCCTATTCCGGCAAAGACCCTCTTCTCCCTTGGCTAAGAGGGATTAGAAGAATGAACGACTCACTCCCACCTCACACTCTAAAGGAGAAGCTGCCTCGCTTCTTGCAGAAATGCGCACAGACTTTCGAGTCCGATCGGCGATATCGAAATGACTTACGCTACATTCGAGTTTGGTTAAAGTTGATGGATTTTGTAGATGATCCGAGAGCATTGTTGAGAACAATGGAGGCTAACCAAATTGGGACAAAGCACTCTTTATTCTATCAGGCATATGCTCTCTACTATGAAAAGAACAAGAAATTTGAAGAGGCTGAAAAGATGTACCACTTGGGAGTGCAAAAGCTTGCAGAGCCCATTGGAGAGTTAGAGAAATCGTATGAGCAGTTCCTTCGTCGCCTGGAGAGACACAGGAGACACCAAAACCAGGAAAAGAGAACAACAAAAAGGCCTTTGTCAGATAGGAGTATCCCTCCAAGTGGAGAAAGGAACAAAGAAAATGCGTGCAGGACTGGGGCCAGGCCTATAGAACCTGAGCATGATGCAAATATAAGTAAGCATCAAGTGACTGCTAAAATAGGGTCAGATGAATCCAGGATGTTTGGCAGTGATGATACAGTTGTGGCGAAGTTTGTAGACACCGCCATTGTTGGAAAGTCTGAAGCAGAAGATGCATGTCACCATGGATTGGTGGATCCTACAATAAACATGAAAGAGGCCATGAGCGCCATAAATAACATGTTTCGAGAGCCTTTAGAGACAGCTCCAGTTAGTAGAAAATCACGCCAATGTCAAAGGAGGGACGATAATCTGGGCAACGAATTCAAGGTATTTGTTGATGAAAACTTGGATAATAGAGGCAAATCAGAAGATAAAACGGAAGAGTTGAGTTTGCCAGTGCTGCAGCATAGGAGAACTGTTACCCAGCAGTCTCGCCAAGAACCTTTAAACATTTTCATTGATGATAATAAAGATAGCCCTGATCAGAGTGATGTCCAAAATTCGACAGAAGATTCTACCTCACCTGCTTCACATGGAAATGTTTTTGTGTTTCCAAATCCAAAATATCTTCCAGGTGAAAGCTCTGGTGATTTGGATGTTGGAGGCTCATCTCAACCAAGGTTTAGAGAGGATACCATGGTTTGTAGGTTTGTTGGGTCTACCATTTCGGATGAGCCACAGGTGGAAAATGTTTGCCACCATGGTTTAGTAGACCCCACAATCAACTTAAAGGAAGCCATGGATGATATCAATAACATGTTTGGGAAGCCAATGGAGTTTGTAAGGAAGAGGAGAGTGAAGAAGCAGGACAAAGGAATAGATAGAAAAAATGATTTTGGTGTCTTTTCTATACTTCCTGATGATAACTTGGACCATAAACAAGAGAAGAAAATGCCAGATTTACCACGAAGATCAAGAGATGCTGATTTGTTTGAGGCCACTGTAACAACAAAGGAGGCCATGGATGAGATCAATAAGATGTTTGGAATGCCCTTGGACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

516

Amino Acids

59.34

Weight (kDa)

6.32

Isoelectric Point (pI)

55.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05510
fragaria_vesca FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900
malus_domestica MD01G1156700.v1.1 MD07G1225400.v1.1
prunus_persica Prupe.2G254200_v2.0.a1
pyrus_communis pycom01g17350 pycom07g20410
rosa_chinensis RchiOBHm_Chr1g0370781
rosa_laevigata RLG00000027010
rosa_multiflora Rmu_sc0009474.1_g000004
rosa_roxburghii Rroxscaffold_4G00286230
rosa_rugosa Rorug01G0359700
rosa_samantha Rh1AG368800 Rh1BG332400 Rh1CG346300 Rh1DG363800
rosa_wichuraiana Rw1G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1075
AasI GACNNNNNNGTC 1 cut(s) 172
Acc16I TGCGCA 1 cut(s) 160
Acc36I ACCTGC 1 cut(s) 1075
AccB7I CCANNNNNTGG 1 cut(s) 730
AccI GTMKAC 3 cut(s) 678, 1202, 1254
AciI CCGC 1 cut(s) 684
AclWI GGATC 3 cut(s) 239, 731, 744
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 344, 863, 1042
AcuI CTGAAG 1 cut(s) 723
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 3 cut(s) 469, 521, 730
AflIII ACRYGT 2 cut(s) 780, 1299
AgsI TTSAA 2 cut(s) 350, 868
AjnI CCWGG 5 cut(s) 441, 468, 560, 635, 1114
AluBI AGCT 4 cut(s) 136, 388, 806, 1125
AluI AGCT 4 cut(s) 136, 388, 806, 1125
Alw26I GTCTC 4 cut(s) 442, 451, 794, 978
AlwI GGATC 3 cut(s) 239, 731, 744
AoxI GGCC 7 cut(s) 3, 491, 558, 563, 759, 1479, 1500
ApeKI GCWGC 3 cut(s) 136, 943, 946
ApoI RAATTY 3 cut(s) 344, 863, 1042
AspLEI GCGC 2 cut(s) 161, 770
AspS9I GGNCC 2 cut(s) 558, 1414
AsuHPI GGTGA 4 cut(s) 713, 1056, 1130, 1142
AvaII GGWCC 1 cut(s) 1414
BaeI ACNNNNGTAYC 4 cut(s) 648, 681, 1167, 1200
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 736
BanII GRGCYC 1 cut(s) 399
BbvI GCAGC 3 cut(s) 123, 930, 958
BccI CCATC 1 cut(s) 223
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 5 cut(s) 443, 470, 562, 637, 1116
BciVI GTATCC 2 cut(s) 521, 1168
BclI TGATCA 1 cut(s) 1024
BcoDI GTCTC 4 cut(s) 442, 451, 794, 978
BfmI CTRYAG 2 cut(s) 567, 944
BfoI RGCGCY 1 cut(s) 771
BfuAI ACCTGC 1 cut(s) 1075
BfuI GTATCC 2 cut(s) 521, 1168
BisI GCNGC 3 cut(s) 137, 944, 947
BlsI GCNGC 3 cut(s) 138, 945, 948
Bme1390I CCNGG 5 cut(s) 443, 470, 562, 637, 1116
Bme18I GGWCC 1 cut(s) 1414
BmgT120I GGNCC 2 cut(s) 558, 1414
BmiI GGNNCC 2 cut(s) 559, 738
BmrFI CCNGG 5 cut(s) 443, 470, 562, 637, 1116
BmrI ACTGGG 1 cut(s) 564
BmsI GCATC 4 cut(s) 574, 610, 703, 1453
BmuI ACTGGG 1 cut(s) 564
BplI GAGNNNNNCTC 2 cut(s) 499, 531
BpmI CTGGAG 2 cut(s) 464, 792
Bpu10I CCTNAGC 1 cut(s) 576
BsaJI CCNNGG 8 cut(s) 74, 724, 1160, 1179, 1243, 1281, 1503, 1536
Bsc4I CCNNNNNNNGG 3 cut(s) 469, 521, 730
Bse1I ACTGG 3 cut(s) 559, 809, 938
BseBI CCWGG 5 cut(s) 443, 470, 562, 637, 1116
BseDI CCNNGG 8 cut(s) 74, 724, 1160, 1179, 1243, 1281, 1503, 1536
BseGI GGATG 5 cut(s) 645, 1144, 1219, 1291, 1513
BseLI CCNNNNNNNGG 3 cut(s) 469, 521, 730
BseMII CTCAG 1 cut(s) 567
BseNI ACTGG 3 cut(s) 559, 809, 938
BseRI GAGGAG 1 cut(s) 1348
BseXI GCAGC 3 cut(s) 123, 930, 958
BseYI CCCAGC 1 cut(s) 966
BsgI GTGCAG 1 cut(s) 568
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 7 cut(s) 5, 493, 560, 565, 761, 1481, 1502
BsiEI CGRYCG 1 cut(s) 181
BsiSI CCGG 1 cut(s) 54
BslFI GGGAC 2 cut(s) 301, 857
BslI CCNNNNNNNGG 3 cut(s) 469, 521, 730
BsmAI GTCTC 4 cut(s) 442, 451, 794, 978
BsmFI GGGAC 2 cut(s) 301, 857
BsmI GAATGC 1 cut(s) 1536
BsnI GGCC 7 cut(s) 5, 493, 560, 565, 761, 1481, 1502
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 7 cut(s) 13, 178, 244, 736, 1024, 1454, 1512
Bsp19I CCATGG 5 cut(s) 724, 1179, 1243, 1281, 1503
BspACI CCGC 1 cut(s) 684
BspANI GGCC 7 cut(s) 5, 493, 560, 565, 761, 1481, 1502
BspCNI CTCAG 1 cut(s) 568
BspLI GGNNCC 2 cut(s) 559, 738
BspMAI CTGCAG 1 cut(s) 948
BspMI ACCTGC 1 cut(s) 1075
BspPI GGATC 3 cut(s) 239, 731, 744
BsrI ACTGG 3 cut(s) 559, 809, 938
BssECI CCNNGG 8 cut(s) 74, 724, 1160, 1179, 1243, 1281, 1503, 1536
BssMI GATC 7 cut(s) 13, 178, 244, 736, 1024, 1454, 1512
BssT1I CCWWGG 8 cut(s) 74, 724, 1160, 1179, 1243, 1281, 1503, 1536
Bst2UI CCWGG 5 cut(s) 443, 470, 562, 637, 1116
Bst4CI ACNGT 3 cut(s) 661, 961, 1486
Bst6I CTCTTC 5 cut(s) 23, 72, 345, 918, 1325
BstAPI GCANNNNNTGC 1 cut(s) 713
BstC8I GCNNGC 2 cut(s) 390, 547
BstDEI CTNAG 2 cut(s) 80, 576
BstDSI CCRYGG 5 cut(s) 724, 1179, 1243, 1281, 1503
BstF5I GGATG 5 cut(s) 645, 1144, 1219, 1291, 1513
BstH2I RGCGCY 1 cut(s) 771
BstHHI GCGC 2 cut(s) 161, 770
BstKTI GATC 7 cut(s) 16, 181, 247, 739, 1027, 1457, 1515
BstMAI GTCTC 4 cut(s) 442, 451, 794, 978
BstMBI GATC 7 cut(s) 13, 178, 244, 736, 1024, 1454, 1512
BstMCI CGRYCG 1 cut(s) 181
BstMWI GCNNNNNNNGC 4 cut(s) 394, 713, 767, 943
BstNI CCWGG 5 cut(s) 443, 470, 562, 637, 1116
BstNSI RCATGY 3 cut(s) 720, 784, 1303
BstSCI CCNGG 5 cut(s) 441, 468, 560, 635, 1114
BstSFI CTRYAG 2 cut(s) 567, 944
BstV1I GCAGC 3 cut(s) 123, 930, 958
BstX2I RGATCY 1 cut(s) 736
BstYI RGATCY 1 cut(s) 736
BsuI GTATCC 2 cut(s) 521, 1168
BsuRI GGCC 7 cut(s) 5, 493, 560, 565, 761, 1481, 1502
BtgI CCRYGG 5 cut(s) 724, 1179, 1243, 1281, 1503
BtsCI GGATG 5 cut(s) 645, 1144, 1219, 1291, 1513
BtsI GCAGTG 1 cut(s) 655
BtsIMutI CAGTG 3 cut(s) 655, 945, 1482
BveI ACCTGC 1 cut(s) 1075
Cac8I GCNNGC 2 cut(s) 390, 547
CfoI GCGC 2 cut(s) 161, 770
Cfr13I GGNCC 2 cut(s) 558, 1414
Csp6I GTAC 1 cut(s) 367
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 2 cut(s) 80, 576
DpnI GATC 7 cut(s) 15, 180, 246, 738, 1026, 1456, 1514
DpnII GATC 7 cut(s) 13, 178, 244, 736, 1024, 1454, 1512
DraI TTTAAA 1 cut(s) 990
DrdI GACNNNNNNGTC 1 cut(s) 172
DseDI GACNNNNNNGTC 1 cut(s) 172
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 5 cut(s) 23, 72, 345, 918, 1325
EarI CTCTTC 5 cut(s) 23, 72, 345, 918, 1325
Eco130I CCWWGG 8 cut(s) 74, 724, 1160, 1179, 1243, 1281, 1503, 1536
Eco147I AGGCCT 2 cut(s) 493, 565
Eco24I GRGCYC 1 cut(s) 399
Eco32I GATATC 2 cut(s) 188, 1291
Eco47I GGWCC 1 cut(s) 1414
Eco57I CTGAAG 1 cut(s) 723
EcoRI GAATTC 1 cut(s) 863
EcoRII CCWGG 5 cut(s) 441, 468, 560, 635, 1114
EcoRV GATATC 2 cut(s) 188, 1291
EcoT14I CCWWGG 8 cut(s) 74, 724, 1160, 1179, 1243, 1281, 1503, 1536
EcoT22I ATGCAT 1 cut(s) 718
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 8 cut(s) 74, 724, 1160, 1179, 1243, 1281, 1503, 1536
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 2 cut(s) 301, 857
FauNDI CATATG 1 cut(s) 316
FbaI TGATCA 1 cut(s) 1024
FblI GTMKAC 3 cut(s) 678, 1202, 1254
Fnu4HI GCNGC 3 cut(s) 137, 944, 947
FokI GGATG 5 cut(s) 652, 1151, 1226, 1298, 1520
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 3 cut(s) 137, 944, 947
FspAI RTGCGCAY 1 cut(s) 160
FspI TGCGCA 1 cut(s) 160
GlaI GCGC 2 cut(s) 160, 769
GluI GCNGC 3 cut(s) 137, 944, 947
GsaI CCCAGC 1 cut(s) 970
GsuI CTGGAG 2 cut(s) 464, 792
HaeII RGCGCY 1 cut(s) 771
HaeIII GGCC 7 cut(s) 5, 493, 560, 565, 761, 1481, 1502
HapII CCGG 1 cut(s) 54
HhaI GCGC 2 cut(s) 161, 770
Hin6I GCGC 2 cut(s) 159, 768
HinP1I GCGC 2 cut(s) 159, 768
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 4 cut(s) 104, 173, 632, 1055
HpaII CCGG 1 cut(s) 54
HphI GGTGA 4 cut(s) 713, 1056, 1130, 1142
Hpy166II GTNNAC 3 cut(s) 679, 1203, 1255
Hpy188I TCNGA 8 cut(s) 178, 249, 502, 628, 703, 910, 1029, 1213
Hpy188III TCNNGA 3 cut(s) 788, 1400, 1458
Hpy8I GTNNAC 3 cut(s) 679, 1203, 1255
Hpy99I CGWCG 1 cut(s) 441
HpyAV CCTTC 1 cut(s) 443
HpyCH4III ACNGT 3 cut(s) 661, 961, 1486
HpyCH4V TGCA 7 cut(s) 151, 382, 392, 549, 587, 716, 946
HpyF10VI GCNNNNNNNGC 4 cut(s) 394, 713, 767, 943
HpyF3I CTNAG 2 cut(s) 80, 576
HspAI GCGC 2 cut(s) 159, 768
Ksp22I TGATCA 1 cut(s) 1024
Kzo9I GATC 7 cut(s) 13, 178, 244, 736, 1024, 1454, 1512
LmnI GCTCC 1 cut(s) 811
Lsp1109I GCAGC 3 cut(s) 123, 930, 958
LweI GCATC 4 cut(s) 574, 610, 703, 1453
MaeIII GTNAC 4 cut(s) 607, 719, 961, 1486
MalI GATC 7 cut(s) 15, 180, 246, 738, 1026, 1456, 1514
MboI GATC 7 cut(s) 13, 178, 244, 736, 1024, 1454, 1512
MflI RGATCY 1 cut(s) 736
MhlI GDGCHC 1 cut(s) 399
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 282, 344, 863, 1042
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 98, 182
MmeI TCCRAC 2 cut(s) 673, 1123
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 718
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 29, 221, 989, 1271
MslI CAYNNNNRTG 2 cut(s) 829, 1080
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 5 cut(s) 443, 470, 562, 637, 1116
Mva1269I GAATGC 1 cut(s) 1536
MvaI CCWGG 5 cut(s) 443, 470, 562, 637, 1116
MwoI GCNNNNNNNGC 4 cut(s) 394, 713, 767, 943
NcoI CCATGG 5 cut(s) 724, 1179, 1243, 1281, 1503
NdeI CATATG 1 cut(s) 316
NdeII GATC 7 cut(s) 13, 178, 244, 736, 1024, 1454, 1512
NlaIV GGNNCC 2 cut(s) 559, 738
NmuCI GTSAC 2 cut(s) 607, 719
NsbI TGCGCA 1 cut(s) 160
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 3 cut(s) 720, 784, 1303
PaqCI CACCTGC 1 cut(s) 1075
PceI AGGCCT 2 cut(s) 493, 565
PciI ACATGT 2 cut(s) 780, 1299
PcsI WCGNNNNNNNCGW 2 cut(s) 187, 208
PctI GAATGC 1 cut(s) 1536
PfeI GAWTC 2 cut(s) 632, 1055
PflMI CCANNNNNTGG 1 cut(s) 730
PfoI TCCNGGA 1 cut(s) 635
PkrI GCNGC 3 cut(s) 138, 945, 948
Ple19I CGATCG 1 cut(s) 181
PleI GAGTC 2 cut(s) 98, 181
PpsI GAGTC 2 cut(s) 98, 181
PscI ACATGT 2 cut(s) 780, 1299
Psp6I CCWGG 5 cut(s) 441, 468, 560, 635, 1114
PspFI CCCAGC 1 cut(s) 966
PspGI CCWGG 5 cut(s) 441, 468, 560, 635, 1114
PspN4I GGNNCC 2 cut(s) 559, 738
PspPI GGNCC 2 cut(s) 558, 1414
PsrI GAACNNNNNNTAC 2 cut(s) 413, 445
PstI CTGCAG 1 cut(s) 948
PsuI RGATCY 1 cut(s) 736
PvuI CGATCG 1 cut(s) 181
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
RseI CAYNNNNRTG 2 cut(s) 829, 1080
SaqAI TTAA 4 cut(s) 29, 221, 989, 1271
SatI GCNGC 3 cut(s) 137, 944, 947
Sau3AI GATC 7 cut(s) 13, 178, 244, 736, 1024, 1454, 1512
Sau96I GGNCC 2 cut(s) 558, 1414
SchI GAGTC 2 cut(s) 98, 182
ScrFI CCNGG 5 cut(s) 443, 470, 562, 637, 1116
SduI GDGCHC 1 cut(s) 399
SfaNI GCATC 4 cut(s) 574, 610, 703, 1453
SfcI CTRYAG 2 cut(s) 567, 944
SinI GGWCC 1 cut(s) 1414
SmiMI CAYNNNNRTG 2 cut(s) 829, 1080
Sse9I AATT 4 cut(s) 282, 344, 863, 1042
SseBI AGGCCT 2 cut(s) 493, 565
SsiI CCGC 1 cut(s) 684
StuI AGGCCT 2 cut(s) 493, 565
StyD4I CCNGG 5 cut(s) 441, 468, 560, 635, 1114
StyI CCWWGG 8 cut(s) 74, 724, 1160, 1179, 1243, 1281, 1503, 1536
TaaI ACNGT 3 cut(s) 661, 961, 1486
TaqI TCGA 5 cut(s) 171, 190, 211, 787, 1046
TasI AATT 4 cut(s) 282, 344, 863, 1042
TfiI GAWTC 2 cut(s) 632, 1055
Tru1I TTAA 4 cut(s) 29, 221, 989, 1271
Tru9I TTAA 4 cut(s) 29, 221, 989, 1271
TscAI CASTG 3 cut(s) 655, 945, 1489
TseFI GTSAC 2 cut(s) 607, 719
TseI GCWGC 3 cut(s) 136, 943, 946
Tsp45I GTSAC 2 cut(s) 607, 719
TspDTI ATGAA 6 cut(s) 113, 345, 645, 767, 897, 988
TspGWI ACGGA 1 cut(s) 935
TspRI CASTG 3 cut(s) 655, 945, 1489
Van91I CCANNNNNTGG 1 cut(s) 730
VpaK11BI GGWCC 1 cut(s) 1414
XapI RAATTY 3 cut(s) 344, 863, 1042
XceI RCATGY 3 cut(s) 720, 784, 1303
XmiI GTMKAC 3 cut(s) 678, 1202, 1254
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.