Rh1AG368800

Mad3/BUB1 hoMad3/BUB1 homology region 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0010450
Physical Location & Seq
Forward (+)
60302390 .. 60304943
2554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG368800.1

Sequence Viewer

Length: 1323 bp
ATGGATTTTGTGGAGGATCCGAGAGAGGTTTTGAGGACGATGGAGGCGGATCGGATTGGGACGAAGCACCCTCTTTTCTACCAGGCATATGCTCTTTACTATGAGAAGAACAAGAAATTTGAGGAGGCTGAGAAGATGTACCATTTGGGGGTGCAGAACCATGCAGATCCCATTGAAGACTTAGAGAAAGCATATGAGCAGTTCCTGCAGCGCATGGAGAGACACAGGAAACGGAAACACCAGGAAAAGAGAACCACAAAAAGAGTAACAGATCGGAGTGGAGCCTCTCGTATTAATAGAGAAAGGAAAAATGAAAAGGAATGCAGGATCCGTGATAGGCCTACAGAAGCCTGGCATGATGGACCTAGAAGTAAGCATCAAGTGACTGCTGGAGGAGGGTCAGATGAACGGATGATGCCTTGCAGCGATGATACAGTCATGGGAAAGTTTGTAGACACTGCCATTGTCGGAAAATCTGAAGCAGAAGATGCTTGTCATCATGGACTGGTGGATCCTACTATAAACATGAAAGAGGCCATGAGTGCCATAAATAGTATGTTTCGAGAGCCTTTAGAGATGGCTCCAGTTAGAAGAGGATCACGCCAATGCCAACAAAAGGAAAATAACACAAACAATGGATTCAAGGTGTTTGTTGATGAAAACTTGGATAAGGGAGGCAAATCAAAAGATAAAGGTGTTTCAATGGTGCAGCAAGGTAGAACTGCTAGCCATCAGTCTCACCGAGAACCTTTAAACATTTTCATTGATGATGATCAAGATAGCCTTGAGCAGAGTGAAGTTCAGAATATGACAGAAGATTCTTCCCCATCTGCTTCACATGGACATGCTTTTGTGTTTCTAAATCCAAAAGATGTTTCAAGTGAAAGTCCTAACGGTTTGGATGTCGAAGGTTCATCTAGACCAAGGTTTAGAGAGGATACGATGATGGTTCGTAGGATTGTTGGCACCACCATTTCGGAGGACCCGCAGGTGGAAAACGCTTGCCACCATGGCTTAGTTGAACCAACTATCAACTTAAAGGAGGCCATGGATGATATCAATAACATGTTTGGCAAGCCAATAGATTTTGTTAGAAGCAGAAGAGTTAAAAGGCAGGACAAAGTACCAGAGAAGAAACAAGATCTTGGTGGGTTTTGTATACTTCCTGACGATTATGACGATGATAACTTGGAACAACAAGTGCAGAAAATGAAAGATCTACCGGGAAAATCAAAAGATTCCGATCTGTTTGAGACTACTGTTTTTACAAAGCAGGCCATGGATGATATCAATAAGATGTTTGGAATGCCACTGGACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

440

Amino Acids

50.51

Weight (kDa)

5.75

Isoelectric Point (pI)

53.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 1 - 51 1.1e-11 Mad3/BUB1 homology region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05510
fragaria_vesca FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900
malus_domestica MD01G1156700.v1.1 MD07G1225400.v1.1
prunus_persica Prupe.2G254200_v2.0.a1
pyrus_communis pycom01g17350 pycom07g20410
rosa_chinensis RchiOBHm_Chr1g0370781
rosa_laevigata RLG00000027010
rosa_multiflora Rmu_sc0009474.1_g000004
rosa_roxburghii Rroxscaffold_4G00286230
rosa_rugosa Rorug01G0359700
rosa_samantha Rh1AG368800 Rh1BG332400 Rh1CG346300 Rh1DG363800
rosa_wichuraiana Rw1G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 979
Acc36I ACCTGC 1 cut(s) 979
AccB1I GGYRCC 1 cut(s) 965
AccI GTMKAC 2 cut(s) 453, 1159
AciI CCGC 2 cut(s) 47, 986
AclWI GGATC 9 cut(s) 11, 24, 57, 161, 322, 335, 506, 519, 604
AcsI RAATTY 1 cut(s) 116
AcuI CTGAAG 1 cut(s) 498
AfaI GTAC 2 cut(s) 140, 1125
AfiI CCNNNNNNNGG 3 cut(s) 148, 616, 991
AflIII ACRYGT 1 cut(s) 1065
AgsI TTSAA 5 cut(s) 176, 643, 702, 879, 1022
AjnI CCWGG 3 cut(s) 81, 240, 350
AjuI GAANNNNNNNTTGG 2 cut(s) 859, 891
Alw26I GTCTC 3 cut(s) 214, 741, 1247
AlwI GGATC 9 cut(s) 11, 24, 57, 161, 322, 335, 506, 519, 604
AlwNI CAGNNNCTG 1 cut(s) 205
AoxI GGCC 4 cut(s) 338, 534, 1044, 1275
ApeKI GCWGC 3 cut(s) 208, 423, 709
ApoI RAATTY 1 cut(s) 116
AseI ATTAAT 1 cut(s) 294
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 2 cut(s) 362, 982
AsuC2I CCSGG 1 cut(s) 1224
AsuHPI GGTGA 1 cut(s) 731
AsuNHI GCTAGC 1 cut(s) 725
AvaII GGWCC 2 cut(s) 362, 982
BamHI GGATCC 3 cut(s) 16, 327, 511
BanI GGYRCC 1 cut(s) 965
BbsI GAAGAC 1 cut(s) 183
BbvI GCAGC 3 cut(s) 220, 435, 721
BccI CCATC 6 cut(s) 34, 353, 571, 738, 835, 940
BciT130I CCWGG 3 cut(s) 83, 242, 352
BciVI GTATCC 1 cut(s) 931
BclI TGATCA 1 cut(s) 772
BcnI CCSGG 1 cut(s) 1224
BcoDI GTCTC 3 cut(s) 214, 741, 1247
BfaI CTAG 3 cut(s) 366, 726, 918
BfmI CTRYAG 2 cut(s) 206, 342
BfuAI ACCTGC 1 cut(s) 979
BfuI GTATCC 1 cut(s) 931
BglI GCCNNNNNGGC 1 cut(s) 1011
BglII AGATCT 2 cut(s) 1141, 1216
BisI GCNGC 3 cut(s) 209, 424, 710
BlsI GCNGC 3 cut(s) 210, 425, 711
Bme1390I CCNGG 4 cut(s) 83, 242, 352, 1224
Bme18I GGWCC 2 cut(s) 362, 982
BmgT120I GGNCC 2 cut(s) 362, 982
BmiI GGNNCC 7 cut(s) 18, 283, 329, 513, 582, 967, 984
BmrFI CCNGG 4 cut(s) 83, 242, 352, 1224
BmsI GCATC 3 cut(s) 385, 405, 478
BmtI GCTAGC 1 cut(s) 729
BpiI GAAGAC 1 cut(s) 183
BpmI CTGGAG 2 cut(s) 411, 567
BpuEI CTTGAG 1 cut(s) 806
BpuMI CCSGG 1 cut(s) 1224
BsaBI GATNNNNATC 2 cut(s) 771, 1242
BsaJI CCNNGG 4 cut(s) 923, 1009, 1047, 1278
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 616, 991
Bse1I ACTGG 3 cut(s) 510, 584, 1317
Bse8I GATNNNNATC 2 cut(s) 771, 1242
BseBI CCWGG 3 cut(s) 83, 242, 352
BseDI CCNNGG 4 cut(s) 923, 1009, 1047, 1278
BseGI GGATG 4 cut(s) 417, 907, 1057, 1288
BseJI GATNNNNATC 2 cut(s) 771, 1242
BseLI CCNNNNNNNGG 3 cut(s) 148, 616, 991
BseMII CTCAG 1 cut(s) 120
BseNI ACTGG 3 cut(s) 510, 584, 1317
BseRI GAGGAG 2 cut(s) 137, 408
BseXI GCAGC 3 cut(s) 220, 435, 721
BsgI GTGCAG 3 cut(s) 173, 728, 1223
BshFI GGCC 4 cut(s) 340, 536, 1046, 1277
BshNI GGYRCC 1 cut(s) 965
BsiSI CCGG 1 cut(s) 1223
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 3 cut(s) 148, 616, 991
BsmAI GTCTC 3 cut(s) 214, 741, 1247
BsmFI GGGAC 1 cut(s) 73
BsmI GAATGC 2 cut(s) 326, 1311
BsnI GGCC 4 cut(s) 340, 536, 1046, 1277
Bsp19I CCATGG 3 cut(s) 1009, 1047, 1278
BspACI CCGC 2 cut(s) 47, 986
BspANI GGCC 4 cut(s) 340, 536, 1046, 1277
BspCNI CTCAG 1 cut(s) 121
BspLI GGNNCC 7 cut(s) 18, 283, 329, 513, 582, 967, 984
BspMAI CTGCAG 1 cut(s) 210
BspMI ACCTGC 1 cut(s) 979
BspOI GCTAGC 1 cut(s) 729
BspPI GGATC 9 cut(s) 11, 24, 57, 161, 322, 335, 506, 519, 604
BspT107I GGYRCC 1 cut(s) 965
BsrI ACTGG 3 cut(s) 510, 584, 1317
BssECI CCNNGG 4 cut(s) 923, 1009, 1047, 1278
BssNAI GTATAC 1 cut(s) 1160
BssT1I CCWWGG 4 cut(s) 923, 1009, 1047, 1278
Bst1107I GTATAC 1 cut(s) 1160
Bst2UI CCWGG 3 cut(s) 83, 242, 352
Bst4CI ACNGT 3 cut(s) 436, 896, 1261
Bst6I CTCTTC 2 cut(s) 586, 1096
BstAPI GCANNNNNTGC 2 cut(s) 205, 488
BstC8I GCNNGC 4 cut(s) 727, 1003, 1076, 1275
BstDEI CTNAG 3 cut(s) 129, 181, 1015
BstDSI CCRYGG 3 cut(s) 1009, 1047, 1278
BstF5I GGATG 4 cut(s) 417, 907, 1057, 1288
BstHHI GCGC 1 cut(s) 213
BstMAI GTCTC 3 cut(s) 214, 741, 1247
BstMWI GCNNNNNNNGC 4 cut(s) 205, 488, 542, 1011
BstNI CCWGG 3 cut(s) 83, 242, 352
BstNSI RCATGY 2 cut(s) 848, 1069
BstSCI CCNGG 4 cut(s) 81, 240, 350, 1222
BstSFI CTRYAG 2 cut(s) 206, 342
BstV1I GCAGC 3 cut(s) 220, 435, 721
BstV2I GAAGAC 1 cut(s) 183
BstX2I RGATCY 6 cut(s) 16, 166, 327, 511, 1141, 1216
BstYI RGATCY 6 cut(s) 16, 166, 327, 511, 1141, 1216
BstZ17I GTATAC 1 cut(s) 1160
BsuI GTATCC 1 cut(s) 931
BsuRI GGCC 4 cut(s) 340, 536, 1046, 1277
BtgI CCRYGG 3 cut(s) 1009, 1047, 1278
BtgZI GCGATG 1 cut(s) 441
BtsCI GGATG 4 cut(s) 417, 907, 1057, 1288
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 2 cut(s) 456, 1310
BveI ACCTGC 1 cut(s) 979
Cac8I GCNNGC 4 cut(s) 727, 1003, 1076, 1275
CaiI CAGNNNCTG 1 cut(s) 205
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 2 cut(s) 362, 982
Csp6I GTAC 2 cut(s) 139, 1124
CviQI GTAC 2 cut(s) 139, 1124
DdeI CTNAG 3 cut(s) 129, 181, 1015
DraI TTTAAA 1 cut(s) 753
Eam1104I CTCTTC 2 cut(s) 586, 1096
EarI CTCTTC 2 cut(s) 586, 1096
EciI GGCGGA 1 cut(s) 62
Eco130I CCWWGG 4 cut(s) 923, 1009, 1047, 1278
Eco147I AGGCCT 1 cut(s) 340
Eco32I GATATC 2 cut(s) 1057, 1288
Eco47I GGWCC 2 cut(s) 362, 982
Eco57I CTGAAG 1 cut(s) 498
EcoO109I RGGNCCY 1 cut(s) 982
EcoRII CCWGG 3 cut(s) 81, 240, 350
EcoRV GATATC 2 cut(s) 1057, 1288
EcoT14I CCWWGG 4 cut(s) 923, 1009, 1047, 1278
ErhI CCWWGG 4 cut(s) 923, 1009, 1047, 1278
FalI AAGNNNNNCTT 2 cut(s) 768, 800
FaqI GGGAC 1 cut(s) 73
FauI CCCGC 1 cut(s) 993
FauNDI CATATG 2 cut(s) 88, 193
FbaI TGATCA 1 cut(s) 772
FblI GTMKAC 2 cut(s) 453, 1159
Fnu4HI GCNGC 3 cut(s) 209, 424, 710
FokI GGATG 4 cut(s) 424, 914, 1064, 1295
Fsp4HI GCNGC 3 cut(s) 209, 424, 710
FspBI CTAG 3 cut(s) 366, 726, 918
GlaI GCGC 1 cut(s) 212
GluI GCNGC 3 cut(s) 209, 424, 710
GsuI CTGGAG 2 cut(s) 411, 567
HaeIII GGCC 4 cut(s) 340, 536, 1046, 1277
HapII CCGG 1 cut(s) 1223
HhaI GCGC 1 cut(s) 213
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HinfI GANTC 3 cut(s) 639, 818, 1238
HpaII CCGG 1 cut(s) 1223
HphI GGTGA 1 cut(s) 731
Hpy166II GTNNAC 2 cut(s) 454, 1160
Hpy188I TCNGA 9 cut(s) 21, 54, 276, 403, 470, 478, 804, 979, 1243
Hpy188III TCNNGA 4 cut(s) 563, 776, 918, 1166
Hpy8I GTNNAC 2 cut(s) 454, 1160
HpyAV CCTTC 1 cut(s) 902
HpyCH4III ACNGT 3 cut(s) 436, 896, 1261
HpyCH4V TGCA 7 cut(s) 154, 164, 208, 324, 423, 709, 1204
HpyF10VI GCNNNNNNNGC 4 cut(s) 205, 488, 542, 1011
HpyF3I CTNAG 3 cut(s) 129, 181, 1015
HspAI GCGC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 772
LmnI GCTCC 2 cut(s) 281, 586
Lsp1109I GCAGC 3 cut(s) 220, 435, 721
LweI GCATC 3 cut(s) 385, 405, 478
MaeI CTAG 3 cut(s) 366, 726, 918
MaeIII GTNAC 2 cut(s) 265, 382
MboII GAAGA 9 cut(s) 118, 145, 188, 497, 603, 813, 827, 1113, 1144
MflI RGATCY 6 cut(s) 16, 166, 327, 511, 1141, 1216
MluCI AATT 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 448
MseI TTAA 4 cut(s) 294, 752, 1037, 1107
MslI CAYNNNNRTG 2 cut(s) 604, 843
MspI CCGG 1 cut(s) 1223
MspR9I CCNGG 4 cut(s) 83, 242, 352, 1224
Mva1269I GAATGC 2 cut(s) 326, 1311
MvaI CCWGG 3 cut(s) 83, 242, 352
MwoI GCNNNNNNNGC 4 cut(s) 205, 488, 542, 1011
NciI CCSGG 1 cut(s) 1224
NcoI CCATGG 3 cut(s) 1009, 1047, 1278
NdeI CATATG 2 cut(s) 88, 193
NheI GCTAGC 1 cut(s) 725
NlaIV GGNNCC 7 cut(s) 18, 283, 329, 513, 582, 967, 984
NmuCI GTSAC 1 cut(s) 382
NspI RCATGY 2 cut(s) 848, 1069
PaqCI CACCTGC 1 cut(s) 979
PceI AGGCCT 1 cut(s) 340
PciI ACATGT 1 cut(s) 1065
PcsI WCGNNNNNNNCGW 1 cut(s) 1176
PctI GAATGC 2 cut(s) 326, 1311
PfeI GAWTC 3 cut(s) 639, 818, 1238
PkrI GCNGC 3 cut(s) 210, 425, 711
PpuMI RGGWCCY 1 cut(s) 982
PscI ACATGT 1 cut(s) 1065
PshBI ATTAAT 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 982
Psp6I CCWGG 3 cut(s) 81, 240, 350
PspGI CCWGG 3 cut(s) 81, 240, 350
PspN4I GGNNCC 7 cut(s) 18, 283, 329, 513, 582, 967, 984
PspPI GGNCC 2 cut(s) 362, 982
PspPPI RGGWCCY 1 cut(s) 982
PstI CTGCAG 1 cut(s) 210
PstNI CAGNNNCTG 1 cut(s) 205
PsuI RGATCY 6 cut(s) 16, 166, 327, 511, 1141, 1216
RsaI GTAC 2 cut(s) 140, 1125
RsaNI GTAC 2 cut(s) 139, 1124
RseI CAYNNNNRTG 2 cut(s) 604, 843
SaqAI TTAA 4 cut(s) 294, 752, 1037, 1107
SatI GCNGC 3 cut(s) 209, 424, 710
Sau96I GGNCC 2 cut(s) 362, 982
ScrFI CCNGG 4 cut(s) 83, 242, 352, 1224
SetI ASST 9 cut(s) 30, 367, 648, 697, 718, 751, 913, 929, 993
SfaNI GCATC 3 cut(s) 385, 405, 478
SfcI CTRYAG 2 cut(s) 206, 342
SinI GGWCC 2 cut(s) 362, 982
SmiMI CAYNNNNRTG 2 cut(s) 604, 843
SmlI CTYRAG 1 cut(s) 785
SmoI CTYRAG 1 cut(s) 785
Sse9I AATT 1 cut(s) 116
SseBI AGGCCT 1 cut(s) 340
SsiI CCGC 2 cut(s) 47, 986
SspMI CTAG 3 cut(s) 366, 726, 918
StuI AGGCCT 1 cut(s) 340
StyD4I CCNGG 4 cut(s) 81, 240, 350, 1222
StyI CCWWGG 4 cut(s) 923, 1009, 1047, 1278
TaaI ACNGT 3 cut(s) 436, 896, 1261
TaqI TCGA 2 cut(s) 562, 906
TasI AATT 1 cut(s) 116
TfiI GAWTC 3 cut(s) 639, 818, 1238
Tru1I TTAA 4 cut(s) 294, 752, 1037, 1107
Tru9I TTAA 4 cut(s) 294, 752, 1037, 1107
TscAI CASTG 2 cut(s) 463, 1317
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 3 cut(s) 208, 423, 709
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 7 cut(s) 327, 420, 542, 672, 751, 903, 1226
TspGWI ACGGA 3 cut(s) 247, 320, 424
TspRI CASTG 2 cut(s) 463, 1317
VpaK11BI GGWCC 2 cut(s) 362, 982
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 1 cut(s) 116
XbaI TCTAGA 1 cut(s) 917
XceI RCATGY 2 cut(s) 848, 1069
XmiI GTMKAC 2 cut(s) 453, 1159
XspI CTAG 3 cut(s) 366, 726, 918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.