Rw1G032470

Mad3/BUB1 hoMad3/BUB1 homology region 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010450
Physical Location & Seq
Forward (+)
60720666 .. 60723085
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032470.1

Sequence Viewer

Length: 1551 bp
ATGGCCAGCAACGATCTCTTCACGTCCTTAATCTCCGACATCAAATCCTACTCCGGCAAAGACCCTCTTCTCCCTTGGCTAAGAGGGATTCGAAGACTGCAAGACTCACTCCCGCAGAACATTCTAGAGGACAAGCTGCCCCGGTTCTTGCAGAAATGCGCGCAGAGCTTCCAGTCCGACGAGCGCTACCGGAACGACCCGCGGTACCTCCGAGTCTGGTTAAAGCTGATGGATTTTGTGGAGGATCCGAGAGAGGTTTTGAGGACGATGGAGGCGGATCGGATTGGGACGAAGCACCCTCTTTTCTACCAGGCATATGCTCTTTACTATGAGAAGAACAAGAAATTTGAGGAGGCTGAGAAGATGTACCATTTGGGGGTGCAGAACCATGCAGATCCCATTGAAGACTTAGAGAAAGCATATGAGCAGTTCCTGCAGCGCATGGAGAGACACAGGAAACGGAAACACCAGGAAAAGAGAACCACAAAAAGAGTAACAGATCGGAGTGGAGCCTCTCGTATTAATAGAGAAAGGAAAAATGAAAAGGAATGCAGGATCCGTGATAGGCCTACAGAAGCCTGGCATGATGGACCTAGAAGTAAGCATCAAGTGACTGCTGGAGGAGGGTCAGATGAACGGATGATGCCTTGCAGCGATGATACAGTCATGGGAAAGTTTGTAGACACTGCCATTGTCGGAAAATCTGAAGCAGAAGATGCTTGTCATCATGGACTGGTGGATCCTACTATAAACATGAAAGAGGCCATGAGTGCCATAAATAGTATGTTTCGAGAGCCTTTAGAGATGGCTCCAGTTAGAAGAGGATCACGCCAATGCCAACAAAAGGAAAATAACACAAACAATGGATTCAAGGTGTTTGTTGATGAAAACTTGGATAAGGGAGGCAAATCAAAAGATAAAGGTGTTTCAATGGTGCAGCAAGGTAGAACTGCTAGCCATCAGTCTCACCGAGAACCTTTAAACATTTTCATTGATGATGATCAAGATAGCCTTGAGCAGAGTGAAGTTCAGAATATGACAGAAGATTCTTCCCCATCTGCTTCACATGGACATGCTTTTGTGTTTCTAAATCCAAAAGATGTTTCAAGTGAAAGTCCTAATGGTTTGGATGTCGAAGGTTCATCTAGACCAAGGTTTAGAGAGGATACGATGATGGTTCGTAGGATTGTTGGGACCACCATTTCGGAGGACCCGCAGGTGGAAAACGCTTGCCACCATGGCTTAGTTGAACCAACTATCAACTTAAAGGAGGCCATGGATGATATCAATAACATGTTTGGCAAGCCAATAGATTTTGTTAGAAGCAGAAGAGTTAAAAGGCAGGACAAAGTACCAGAGAAGAAACAAGATCTTGGTGGGTTTTGTATACTTCCTGACGATGATGACGATGATAACTTGGAACAACAAGTGCAGAAAATGAAAGATCTACCGGGAAAATCAAAAGATTCCGATCTGTTTGAGACTACTGTTTTTACAAAGCAGGCCATGGATGATATCAATAAGATGTTTGGAATGCCACTGGACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

516

Amino Acids

59.5

Weight (kDa)

5.99

Isoelectric Point (pI)

53.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 10 - 127 1.1e-37 Mad3/BUB1 homology region 1
Mad3_BUB1_I_2 PF17014 63 - 163 6e-06 Putative Mad3/BUB1 like region 1 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05510
fragaria_vesca FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900
malus_domestica MD01G1156700.v1.1 MD07G1225400.v1.1
prunus_persica Prupe.2G254200_v2.0.a1
pyrus_communis pycom01g17350 pycom07g20410
rosa_chinensis RchiOBHm_Chr1g0370781
rosa_laevigata RLG00000027010
rosa_multiflora Rmu_sc0009474.1_g000004
rosa_roxburghii Rroxscaffold_4G00286230
rosa_rugosa Rorug01G0359700
rosa_samantha Rh1AG368800 Rh1BG332400 Rh1CG346300 Rh1DG363800
rosa_wichuraiana Rw1G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1207
Acc36I ACCTGC 1 cut(s) 1207
Acc65I GGTACC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 204
AccI GTMKAC 2 cut(s) 681, 1387
AccII CGCG 2 cut(s) 161, 202
AciI CCGC 5 cut(s) 113, 200, 202, 275, 1214
AclWI GGATC 9 cut(s) 239, 252, 285, 389, 550, 563, 734, 747, 832
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 344
AcuI CTGAAG 1 cut(s) 726
AfaI GTAC 3 cut(s) 206, 368, 1353
AfeI AGCGCT 1 cut(s) 185
AfiI CCNNNNNNNGG 3 cut(s) 376, 844, 1219
AflIII ACRYGT 1 cut(s) 1293
AgsI TTSAA 5 cut(s) 404, 871, 930, 1107, 1250
AjiI CACGTC 1 cut(s) 24
AjnI CCWGG 3 cut(s) 309, 468, 578
AjuI GAANNNNNNNTTGG 2 cut(s) 1087, 1119
AluBI AGCT 3 cut(s) 136, 168, 226
AluI AGCT 3 cut(s) 136, 168, 226
Alw26I GTCTC 3 cut(s) 442, 969, 1475
AlwI GGATC 9 cut(s) 239, 252, 285, 389, 550, 563, 734, 747, 832
AlwNI CAGNNNCTG 1 cut(s) 433
Aor51HI AGCGCT 1 cut(s) 185
AoxI GGCC 5 cut(s) 3, 566, 762, 1272, 1503
ApeKI GCWGC 4 cut(s) 136, 436, 651, 937
ApoI RAATTY 1 cut(s) 344
ArsI GACNNNNNNTTYG 2 cut(s) 1186, 1218
AseI ATTAAT 1 cut(s) 522
Asp718I GGTACC 1 cut(s) 204
AspLEI GCGC 4 cut(s) 161, 163, 186, 441
AspS9I GGNCC 3 cut(s) 590, 1194, 1210
AsuC2I CCSGG 2 cut(s) 142, 1452
AsuHPI GGTGA 1 cut(s) 959
AsuII TTCGAA 1 cut(s) 91
AsuNHI GCTAGC 1 cut(s) 953
AvaII GGWCC 3 cut(s) 590, 1194, 1210
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 3 cut(s) 244, 555, 739
BanI GGYRCC 1 cut(s) 204
BbsI GAAGAC 2 cut(s) 100, 411
BbvI GCAGC 4 cut(s) 123, 448, 663, 949
BccI CCATC 7 cut(s) 223, 262, 581, 799, 966, 1063, 1168
BciT130I CCWGG 3 cut(s) 311, 470, 580
BciVI GTATCC 1 cut(s) 1159
BclI TGATCA 1 cut(s) 1000
BcnI CCSGG 2 cut(s) 142, 1452
BcoDI GTCTC 3 cut(s) 442, 969, 1475
BfaI CTAG 4 cut(s) 125, 594, 954, 1146
BfmI CTRYAG 2 cut(s) 434, 570
BfoI RGCGCY 1 cut(s) 187
BfuAI ACCTGC 1 cut(s) 1207
BfuI GTATCC 1 cut(s) 1159
BglI GCCNNNNNGGC 1 cut(s) 1239
BglII AGATCT 2 cut(s) 1369, 1444
BisI GCNGC 4 cut(s) 137, 437, 652, 938
BlsI GCNGC 4 cut(s) 138, 438, 653, 939
Bme1390I CCNGG 5 cut(s) 142, 311, 470, 580, 1452
Bme18I GGWCC 3 cut(s) 590, 1194, 1210
BmgBI CACGTC 1 cut(s) 24
BmgT120I GGNCC 3 cut(s) 590, 1194, 1210
BmiI GGNNCC 8 cut(s) 206, 246, 511, 557, 741, 810, 1195, 1212
BmrFI CCNGG 5 cut(s) 142, 311, 470, 580, 1452
BmsI GCATC 3 cut(s) 613, 633, 706
BmtI GCTAGC 1 cut(s) 957
BpiI GAAGAC 2 cut(s) 100, 411
BpmI CTGGAG 2 cut(s) 639, 795
Bpu14I TTCGAA 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 1034
BpuMI CCSGG 2 cut(s) 142, 1452
BsaBI GATNNNNATC 2 cut(s) 999, 1470
BsaJI CCNNGG 7 cut(s) 74, 140, 200, 1151, 1237, 1275, 1506
BsaWI WCCGGW 1 cut(s) 189
Bsc4I CCNNNNNNNGG 3 cut(s) 376, 844, 1219
Bse1I ACTGG 4 cut(s) 172, 738, 812, 1545
Bse8I GATNNNNATC 2 cut(s) 999, 1470
BseBI CCWGG 3 cut(s) 311, 470, 580
BseDI CCNNGG 7 cut(s) 74, 140, 200, 1151, 1237, 1275, 1506
BseGI GGATG 4 cut(s) 645, 1135, 1285, 1516
BseJI GATNNNNATC 2 cut(s) 999, 1470
BseLI CCNNNNNNNGG 3 cut(s) 376, 844, 1219
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 4 cut(s) 172, 738, 812, 1545
BsePI GCGCGC 1 cut(s) 159
BseRI GAGGAG 2 cut(s) 365, 636
BseXI GCAGC 4 cut(s) 123, 448, 663, 949
BsgI GTGCAG 3 cut(s) 401, 956, 1451
Bsh1236I CGCG 2 cut(s) 161, 202
BshFI GGCC 5 cut(s) 5, 568, 764, 1274, 1505
BshNI GGYRCC 1 cut(s) 204
BsiSI CCGG 4 cut(s) 54, 142, 190, 1451
BslFI GGGAC 2 cut(s) 301, 1207
BslI CCNNNNNNNGG 3 cut(s) 376, 844, 1219
BsmAI GTCTC 3 cut(s) 442, 969, 1475
BsmFI GGGAC 2 cut(s) 301, 1207
BsmI GAATGC 2 cut(s) 554, 1539
BsnI GGCC 5 cut(s) 5, 568, 764, 1274, 1505
Bsp119I TTCGAA 1 cut(s) 91
Bsp19I CCATGG 3 cut(s) 1237, 1275, 1506
BspACI CCGC 5 cut(s) 113, 200, 202, 275, 1214
BspANI GGCC 5 cut(s) 5, 568, 764, 1274, 1505
BspCNI CTCAG 1 cut(s) 349
BspFNI CGCG 2 cut(s) 161, 202
BspLI GGNNCC 8 cut(s) 206, 246, 511, 557, 741, 810, 1195, 1212
BspMAI CTGCAG 1 cut(s) 438
BspMI ACCTGC 1 cut(s) 1207
BspOI GCTAGC 1 cut(s) 957
BspPI GGATC 9 cut(s) 239, 252, 285, 389, 550, 563, 734, 747, 832
BspT104I TTCGAA 1 cut(s) 91
BspT107I GGYRCC 1 cut(s) 204
BsrI ACTGG 4 cut(s) 172, 738, 812, 1545
BssECI CCNNGG 7 cut(s) 74, 140, 200, 1151, 1237, 1275, 1506
BssHII GCGCGC 1 cut(s) 159
BssNAI GTATAC 1 cut(s) 1388
BssT1I CCWWGG 5 cut(s) 74, 1151, 1237, 1275, 1506
Bst1107I GTATAC 1 cut(s) 1388
Bst2UI CCWGG 3 cut(s) 311, 470, 580
Bst4CI ACNGT 2 cut(s) 664, 1489
Bst6I CTCTTC 4 cut(s) 23, 72, 814, 1324
BstAPI GCANNNNNTGC 2 cut(s) 433, 716
BstBI TTCGAA 1 cut(s) 91
BstC8I GCNNGC 6 cut(s) 7, 161, 955, 1231, 1304, 1503
BstDEI CTNAG 4 cut(s) 80, 357, 409, 1243
BstDSI CCRYGG 4 cut(s) 200, 1237, 1275, 1506
BstF5I GGATG 4 cut(s) 645, 1135, 1285, 1516
BstFNI CGCG 2 cut(s) 161, 202
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 4 cut(s) 161, 163, 186, 441
BstMAI GTCTC 3 cut(s) 442, 969, 1475
BstMWI GCNNNNNNNGC 5 cut(s) 165, 433, 716, 770, 1239
BstNI CCWGG 3 cut(s) 311, 470, 580
BstNSI RCATGY 2 cut(s) 1076, 1297
BstSCI CCNGG 5 cut(s) 140, 309, 468, 578, 1450
BstSFI CTRYAG 2 cut(s) 434, 570
BstUI CGCG 2 cut(s) 161, 202
BstV1I GCAGC 4 cut(s) 123, 448, 663, 949
BstV2I GAAGAC 2 cut(s) 100, 411
BstX2I RGATCY 6 cut(s) 244, 394, 555, 739, 1369, 1444
BstYI RGATCY 6 cut(s) 244, 394, 555, 739, 1369, 1444
BstZ17I GTATAC 1 cut(s) 1388
BsuI GTATCC 1 cut(s) 1159
BsuRI GGCC 5 cut(s) 5, 568, 764, 1274, 1505
BtgI CCRYGG 4 cut(s) 200, 1237, 1275, 1506
BtgZI GCGATG 1 cut(s) 669
BtrI CACGTC 1 cut(s) 24
BtsCI GGATG 4 cut(s) 645, 1135, 1285, 1516
BtsI GCAGTG 1 cut(s) 684
BtsIMutI CAGTG 2 cut(s) 684, 1538
BveI ACCTGC 1 cut(s) 1207
Cac8I GCNNGC 6 cut(s) 7, 161, 955, 1231, 1304, 1503
CaiI CAGNNNCTG 1 cut(s) 433
CfoI GCGC 4 cut(s) 161, 163, 186, 441
Cfr13I GGNCC 3 cut(s) 590, 1194, 1210
Cfr42I CCGCGG 1 cut(s) 203
Csp6I GTAC 3 cut(s) 205, 367, 1352
CviQI GTAC 3 cut(s) 205, 367, 1352
DdeI CTNAG 4 cut(s) 80, 357, 409, 1243
DraI TTTAAA 1 cut(s) 981
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 23, 72, 814, 1324
EarI CTCTTC 4 cut(s) 23, 72, 814, 1324
EciI GGCGGA 1 cut(s) 290
Eco130I CCWWGG 5 cut(s) 74, 1151, 1237, 1275, 1506
Eco147I AGGCCT 1 cut(s) 568
Eco32I GATATC 2 cut(s) 1285, 1516
Eco47I GGWCC 3 cut(s) 590, 1194, 1210
Eco47III AGCGCT 1 cut(s) 185
Eco57I CTGAAG 1 cut(s) 726
EcoO109I RGGNCCY 1 cut(s) 1210
EcoRII CCWGG 3 cut(s) 309, 468, 578
EcoRV GATATC 2 cut(s) 1285, 1516
EcoT14I CCWWGG 5 cut(s) 74, 1151, 1237, 1275, 1506
ErhI CCWWGG 5 cut(s) 74, 1151, 1237, 1275, 1506
FalI AAGNNNNNCTT 4 cut(s) 51, 83, 996, 1028
FaqI GGGAC 2 cut(s) 301, 1207
FauI CCCGC 3 cut(s) 120, 207, 1221
FauNDI CATATG 2 cut(s) 316, 421
FbaI TGATCA 1 cut(s) 1000
FblI GTMKAC 2 cut(s) 681, 1387
Fnu4HI GCNGC 4 cut(s) 137, 437, 652, 938
FokI GGATG 4 cut(s) 652, 1142, 1292, 1523
Fsp4HI GCNGC 4 cut(s) 137, 437, 652, 938
FspBI CTAG 4 cut(s) 125, 594, 954, 1146
GlaI GCGC 4 cut(s) 160, 162, 185, 440
GluI GCNGC 4 cut(s) 137, 437, 652, 938
GsuI CTGGAG 2 cut(s) 639, 795
HaeII RGCGCY 1 cut(s) 187
HaeIII GGCC 5 cut(s) 5, 568, 764, 1274, 1505
HapII CCGG 4 cut(s) 54, 142, 190, 1451
HhaI GCGC 4 cut(s) 161, 163, 186, 441
Hin6I GCGC 4 cut(s) 159, 161, 184, 439
HinP1I GCGC 4 cut(s) 159, 161, 184, 439
HinfI GANTC 6 cut(s) 88, 104, 213, 867, 1046, 1466
HpaII CCGG 4 cut(s) 54, 142, 190, 1451
HphI GGTGA 1 cut(s) 959
Hpy166II GTNNAC 2 cut(s) 682, 1388
Hpy188III TCNNGA 5 cut(s) 125, 791, 1004, 1146, 1394
Hpy8I GTNNAC 2 cut(s) 682, 1388
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 1 cut(s) 1130
HpyCH4III ACNGT 2 cut(s) 664, 1489
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 9 cut(s) 100, 151, 382, 392, 436, 552, 651, 937, 1432
HpyF10VI GCNNNNNNNGC 5 cut(s) 165, 433, 716, 770, 1239
HpyF3I CTNAG 4 cut(s) 80, 357, 409, 1243
HpySE526I ACGT 1 cut(s) 23
HspAI GCGC 4 cut(s) 159, 161, 184, 439
KpnI GGTACC 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 1000
KspI CCGCGG 1 cut(s) 203
LmnI GCTCC 2 cut(s) 509, 814
Lsp1109I GCAGC 4 cut(s) 123, 448, 663, 949
LweI GCATC 3 cut(s) 613, 633, 706
MaeI CTAG 4 cut(s) 125, 594, 954, 1146
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 2 cut(s) 493, 610
MflI RGATCY 6 cut(s) 244, 394, 555, 739, 1369, 1444
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 344
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 98, 222
MmeI TCCRAC 3 cut(s) 60, 201, 676
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 29, 221, 522, 980, 1265, 1335
MslI CAYNNNNRTG 2 cut(s) 832, 1071
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 4 cut(s) 54, 142, 190, 1451
MspR9I CCNGG 5 cut(s) 142, 311, 470, 580, 1452
Mva1269I GAATGC 2 cut(s) 554, 1539
MvaI CCWGG 3 cut(s) 311, 470, 580
MvnI CGCG 2 cut(s) 161, 202
MwoI GCNNNNNNNGC 5 cut(s) 165, 433, 716, 770, 1239
NciI CCSGG 2 cut(s) 142, 1452
NcoI CCATGG 3 cut(s) 1237, 1275, 1506
NdeI CATATG 2 cut(s) 316, 421
NheI GCTAGC 1 cut(s) 953
NlaIV GGNNCC 8 cut(s) 206, 246, 511, 557, 741, 810, 1195, 1212
NmuCI GTSAC 1 cut(s) 610
NspI RCATGY 2 cut(s) 1076, 1297
NspV TTCGAA 1 cut(s) 91
PaqCI CACCTGC 1 cut(s) 1207
PauI GCGCGC 1 cut(s) 159
PceI AGGCCT 1 cut(s) 568
PciI ACATGT 1 cut(s) 1293
PcsI WCGNNNNNNNCGW 1 cut(s) 1404
PctI GAATGC 2 cut(s) 554, 1539
PfeI GAWTC 4 cut(s) 88, 867, 1046, 1466
PkrI GCNGC 4 cut(s) 138, 438, 653, 939
PleI GAGTC 2 cut(s) 98, 221
PpsI GAGTC 2 cut(s) 98, 221
PpuMI RGGWCCY 1 cut(s) 1210
PscI ACATGT 1 cut(s) 1293
PshBI ATTAAT 1 cut(s) 522
Psp5II RGGWCCY 1 cut(s) 1210
Psp6I CCWGG 3 cut(s) 309, 468, 578
PspGI CCWGG 3 cut(s) 309, 468, 578
PspN4I GGNNCC 8 cut(s) 206, 246, 511, 557, 741, 810, 1195, 1212
PspPI GGNCC 3 cut(s) 590, 1194, 1210
PspPPI RGGWCCY 1 cut(s) 1210
PstI CTGCAG 1 cut(s) 438
PstNI CAGNNNCTG 1 cut(s) 433
PsuI RGATCY 6 cut(s) 244, 394, 555, 739, 1369, 1444
PteI GCGCGC 1 cut(s) 159
RsaI GTAC 3 cut(s) 206, 368, 1353
RsaNI GTAC 3 cut(s) 205, 367, 1352
RseI CAYNNNNRTG 2 cut(s) 832, 1071
SacII CCGCGG 1 cut(s) 203
SaqAI TTAA 6 cut(s) 29, 221, 522, 980, 1265, 1335
SatI GCNGC 4 cut(s) 137, 437, 652, 938
Sau96I GGNCC 3 cut(s) 590, 1194, 1210
SchI GAGTC 2 cut(s) 98, 222
ScrFI CCNGG 5 cut(s) 142, 311, 470, 580, 1452
SfaNI GCATC 3 cut(s) 613, 633, 706
SfcI CTRYAG 2 cut(s) 434, 570
Sfr303I CCGCGG 1 cut(s) 203
SfuI TTCGAA 1 cut(s) 91
SgrBI CCGCGG 1 cut(s) 203
SinI GGWCC 3 cut(s) 590, 1194, 1210
SmiMI CAYNNNNRTG 2 cut(s) 832, 1071
SmlI CTYRAG 1 cut(s) 1013
SmoI CTYRAG 1 cut(s) 1013
Sse9I AATT 1 cut(s) 344
SseBI AGGCCT 1 cut(s) 568
SsiI CCGC 5 cut(s) 113, 200, 202, 275, 1214
SspMI CTAG 4 cut(s) 125, 594, 954, 1146
StuI AGGCCT 1 cut(s) 568
StyD4I CCNGG 5 cut(s) 140, 309, 468, 578, 1450
StyI CCWWGG 5 cut(s) 74, 1151, 1237, 1275, 1506
TaaI ACNGT 2 cut(s) 664, 1489
TaiI ACGT 1 cut(s) 26
TaqI TCGA 3 cut(s) 91, 790, 1134
TasI AATT 1 cut(s) 344
TfiI GAWTC 4 cut(s) 88, 867, 1046, 1466
Tru1I TTAA 6 cut(s) 29, 221, 522, 980, 1265, 1335
Tru9I TTAA 6 cut(s) 29, 221, 522, 980, 1265, 1335
TscAI CASTG 2 cut(s) 691, 1545
TseFI GTSAC 1 cut(s) 610
TseI GCWGC 4 cut(s) 136, 436, 651, 937
Tsp45I GTSAC 1 cut(s) 610
TspDTI ATGAA 7 cut(s) 555, 648, 770, 900, 979, 1131, 1454
TspGWI ACGGA 3 cut(s) 475, 548, 652
TspRI CASTG 2 cut(s) 691, 1545
VpaK11BI GGWCC 3 cut(s) 590, 1194, 1210
VspI ATTAAT 1 cut(s) 522
XapI RAATTY 1 cut(s) 344
XbaI TCTAGA 2 cut(s) 124, 1145
XceI RCATGY 2 cut(s) 1076, 1297
XmiI GTMKAC 2 cut(s) 681, 1387
XspI CTAG 4 cut(s) 125, 594, 954, 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.