MD07G1225400.v1.1

Mad3/BUB1 hoMad3/BUB1 homology region 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010450
Physical Location & Seq
Forward (+)
30185602 .. 30188218
2617 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1225400.v1.1.491

Sequence Viewer

Length: 1542 bp
ATGTCCAACAATGATCTTTTCACCTCCCTAATCTCCGACGTCAAAACCTACTCCGGCAAAGACCCTCTTCTACCATGGCTAAGAGGGATTCGAAAAATGAACGACTCACTCCCGCCTCACACTCTGAAGGAGAAGCTCCCTCGCTTCTTGCAGAAGTGTGCGCAGACGTTCGAGTCCGATCGACGATACCGAAATGATTTGCGCTACATTCGAGTTTGGTTAAAGTTGATGGATTTTGTCGAGGATCCGAGAGCTCTGTTGAGAACAATGGAGGCGAATCAAATCGGGACAAAGCACTCTTTGTTCTACCAGGCATATGCTCTGTACTATGAAAAGAACAAGAAATTTGAGGAAGCCGAGAAGATGTACCACTTGGGAGTGCAAAACCTTGCAGAGCCCATTGGAGCGTTAGAGAAGTCGTACGAGCAGTTCCTTCAACGGATGGAGAGACACAGGAGACACAGAAACCAGGAAAAGAGGACCTTGAAAAGGCCTCTGTCAGATAAGACTATCCCTCCAAATGGAGAAAGGAACAAAGAAAATGCCTGCAGGATCGGCCCCAGGCCTATAGAATCCCAACATGTTCCGTCTAGAAGTGAGCATCAAGTGACTTCTGGAAGAGGGTCAGAGGAACTGAAAATGTTTGGCGGCGATGATACAGTTGTGGTGAAGTTTGTAGACACTGCCATTGTTGGGAAGTCTGAAGCGGAAGATGCGTGTCACCATGGATTGGTGGATCCTACTATAAACATGAAAGAGGCCATGAGCGCCATCAATGACATGTTTCGAGAGCCTATAGAGGCGGCTCCCATTAGCAGAAGATCTCGACAATCCCAACCGAAGAACAATACTGTGGACAGTGGATTCCAAGTATTTGTTGATGACAACTTAGATAATGGAGGGAAATCCGAAAATATTAAGGGAGAATTGAGTAGAACTGCTACCCACCAGTCTCACCAAGAACCTTTAAACATTTTCATTGATGATGATGATAATGACGACAAAGATAGCCCTGATCAGAGTGACATCGGAAATCCGACAGATGATTCTACCTCACCTGCTTCACATGGAAATGTATTCGTGTTTCCAAATCCAAAAGATTTTCCGGGTGAAAGTTCCGGTGATTTGGCTGTTGAAGGCTCATCTCAACCGAGGTTCAGGGAGGATACAATGGTTTGTAGGTTTGTTGGGGCCACCATTTCGGATGAGCCAGAGGAGGTCGAAAATGCTTGCCACCATGGTTTGGTAGACCCTACAATCAACTTAAAGGAAGCCATGGATGATATCAATAACATGTTTGGGAAGCCAATGGAATTTGTAAGAAAGAGGAGAGTGAAAAACCAGGAAAGAGGACTTGATAGGAAAGAAGATTTTGGTGGGTTTTCTATACTTCCTGATGATAACTTGAACCAGAAGCAAGAGCAGAAAATGCCTGATTTACCGCAGAGATCAAGAGATTCAGATTTGTTCGAGGCCACTGTAACAACAAAGGAGGCCATGGATGAGATCAATAAGATGTTTGGAATGCCCCTGGACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

514

Amino Acids

58.64

Weight (kDa)

5.47

Isoelectric Point (pI)

56.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 11 - 127 8.3e-37 Mad3/BUB1 homology region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05510
fragaria_vesca FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900
malus_domestica MD01G1156700.v1.1 MD07G1225400.v1.1
prunus_persica Prupe.2G254200_v2.0.a1
pyrus_communis pycom01g17350 pycom07g20410
rosa_chinensis RchiOBHm_Chr1g0370781
rosa_laevigata RLG00000027010
rosa_multiflora Rmu_sc0009474.1_g000004
rosa_roxburghii Rroxscaffold_4G00286230
rosa_rugosa Rorug01G0359700
rosa_samantha Rh1AG368800 Rh1BG332400 Rh1CG346300 Rh1DG363800
rosa_wichuraiana Rw1G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1066
AasI GACNNNNNNGTC 1 cut(s) 172
AatII GACGTC 1 cut(s) 42
Acc16I TGCGCA 1 cut(s) 162
Acc36I ACCTGC 1 cut(s) 1066
AccB7I CCANNNNNTGG 2 cut(s) 730, 1243
AccI GTMKAC 2 cut(s) 678, 1248
AciI CCGC 5 cut(s) 113, 648, 707, 803, 1442
AclWI GGATC 5 cut(s) 239, 252, 560, 731, 744
AcsI RAATTY 2 cut(s) 344, 1313
AcuI CTGAAG 2 cut(s) 146, 723
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 3 cut(s) 326, 368, 422
AfiI CCNNNNNNNGG 5 cut(s) 489, 521, 693, 730, 1243
AflIII ACRYGT 3 cut(s) 580, 780, 1293
AgsI TTSAA 4 cut(s) 437, 487, 1136, 1408
AjnI CCWGG 5 cut(s) 309, 468, 560, 1341, 1530
AluBI AGCT 2 cut(s) 136, 254
AluI AGCT 2 cut(s) 136, 254
Alw21I GWGCWC 1 cut(s) 256
Alw26I GTCTC 3 cut(s) 442, 451, 957
AlwI GGATC 5 cut(s) 239, 252, 560, 731, 744
AoxI GGCC 7 cut(s) 491, 556, 563, 759, 1191, 1473, 1494
ApoI RAATTY 2 cut(s) 344, 1313
AspLEI GCGC 3 cut(s) 163, 204, 770
AspS9I GGNCC 3 cut(s) 480, 557, 1191
AsuC2I CCSGG 1 cut(s) 1107
AsuHPI GGTGA 7 cut(s) 13, 679, 713, 947, 1047, 1121, 1133
AsuII TTCGAA 1 cut(s) 91
AvaII GGWCC 1 cut(s) 480
BaeI ACNNNNGTAYC 4 cut(s) 648, 681, 1158, 1191
BamHI GGATCC 2 cut(s) 244, 736
BanII GRGCYC 2 cut(s) 256, 399
Bbv12I GWGCWC 1 cut(s) 256
BccI CCATC 3 cut(s) 223, 436, 779
BciT130I CCWGG 5 cut(s) 311, 470, 562, 1343, 1532
BciVI GTATCC 1 cut(s) 1159
BclI TGATCA 1 cut(s) 1015
BcnI CCSGG 1 cut(s) 1107
BcoDI GTCTC 3 cut(s) 442, 451, 957
BfaI CTAG 2 cut(s) 591, 1540
BfmI CTRYAG 3 cut(s) 547, 567, 795
BfoI RGCGCY 1 cut(s) 771
BfuAI ACCTGC 1 cut(s) 1066
BfuI GTATCC 1 cut(s) 1159
BglII AGATCT 1 cut(s) 821
BisI GCNGC 2 cut(s) 649, 804
BlsI GCNGC 2 cut(s) 650, 805
Bme1390I CCNGG 6 cut(s) 311, 470, 562, 1107, 1343, 1532
Bme18I GGWCC 1 cut(s) 480
BmgT120I GGNCC 3 cut(s) 480, 557, 1191
BmiI GGNNCC 5 cut(s) 246, 559, 738, 807, 1192
BmrFI CCNGG 6 cut(s) 311, 470, 562, 1107, 1343, 1532
BmsI GCATC 2 cut(s) 610, 703
Bpu14I TTCGAA 1 cut(s) 91
BpuMI CCSGG 1 cut(s) 1107
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 8 cut(s) 74, 560, 724, 1151, 1237, 1275, 1497, 1530
BsaWI WCCGGW 1 cut(s) 1118
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bsc4I CCNNNNNNNGG 5 cut(s) 489, 521, 693, 730, 1243
Bse1I ACTGG 1 cut(s) 949
BseBI CCWGG 5 cut(s) 311, 470, 562, 1343, 1532
BseDI CCNNGG 8 cut(s) 74, 560, 724, 1151, 1237, 1275, 1497, 1530
BseGI GGATG 4 cut(s) 447, 1210, 1285, 1507
BseLI CCNNNNNNNGG 5 cut(s) 489, 521, 693, 730, 1243
BseNI ACTGG 1 cut(s) 949
BseRI GAGGAG 2 cut(s) 1229, 1342
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 7 cut(s) 493, 558, 565, 761, 1193, 1475, 1496
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 256
BsiSI CCGG 3 cut(s) 54, 1106, 1119
BsiWI CGTACG 1 cut(s) 420
BslFI GGGAC 1 cut(s) 301
BslI CCNNNNNNNGG 5 cut(s) 489, 521, 693, 730, 1243
BsmAI GTCTC 3 cut(s) 442, 451, 957
BsmFI GGGAC 1 cut(s) 301
BsmI GAATGC 1 cut(s) 1530
BsnI GGCC 7 cut(s) 493, 558, 565, 761, 1193, 1475, 1496
Bsp119I TTCGAA 1 cut(s) 91
Bsp1286I GDGCHC 2 cut(s) 256, 399
Bsp143I GATC 9 cut(s) 13, 178, 244, 552, 736, 821, 1015, 1448, 1506
Bsp19I CCATGG 5 cut(s) 74, 724, 1237, 1275, 1497
BspACI CCGC 5 cut(s) 113, 648, 707, 803, 1442
BspANI GGCC 7 cut(s) 493, 558, 565, 761, 1193, 1475, 1496
BspLI GGNNCC 5 cut(s) 246, 559, 738, 807, 1192
BspMAI CTGCAG 1 cut(s) 551
BspMI ACCTGC 1 cut(s) 1066
BspPI GGATC 5 cut(s) 239, 252, 560, 731, 744
BspT104I TTCGAA 1 cut(s) 91
BsrI ACTGG 1 cut(s) 949
BssECI CCNNGG 8 cut(s) 74, 560, 724, 1151, 1237, 1275, 1497, 1530
BssMI GATC 9 cut(s) 13, 178, 244, 552, 736, 821, 1015, 1448, 1506
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 5 cut(s) 74, 724, 1237, 1275, 1497
Bst2UI CCWGG 5 cut(s) 311, 470, 562, 1343, 1532
Bst4CI ACNGT 4 cut(s) 661, 853, 860, 1480
Bst6I CTCTTC 2 cut(s) 72, 613
BstACI GRCGYC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 1429
BstBI TTCGAA 1 cut(s) 91
BstC8I GCNNGC 2 cut(s) 547, 1231
BstDEI CTNAG 2 cut(s) 80, 889
BstDSI CCRYGG 5 cut(s) 74, 724, 1237, 1275, 1497
BstENI CCTNNNNNAGG 1 cut(s) 487
BstF5I GGATG 4 cut(s) 447, 1210, 1285, 1507
BstH2I RGCGCY 1 cut(s) 771
BstHHI GCGC 3 cut(s) 163, 204, 770
BstKTI GATC 9 cut(s) 16, 181, 247, 555, 739, 824, 1018, 1451, 1509
BstMAI GTCTC 3 cut(s) 442, 451, 957
BstMBI GATC 9 cut(s) 13, 178, 244, 552, 736, 821, 1015, 1448, 1506
BstMCI CGRYCG 1 cut(s) 181
BstMWI GCNNNNNNNGC 4 cut(s) 555, 713, 767, 1429
BstNI CCWGG 5 cut(s) 311, 470, 562, 1343, 1532
BstNSI RCATGY 3 cut(s) 584, 784, 1297
BstSCI CCNGG 6 cut(s) 309, 468, 560, 1105, 1341, 1530
BstSFI CTRYAG 3 cut(s) 547, 567, 795
BstX2I RGATCY 3 cut(s) 244, 736, 821
BstYI RGATCY 3 cut(s) 244, 736, 821
BsuI GTATCC 1 cut(s) 1159
BsuRI GGCC 7 cut(s) 493, 558, 565, 761, 1193, 1475, 1496
BtgI CCRYGG 5 cut(s) 74, 724, 1237, 1275, 1497
BtgZI GCGATG 1 cut(s) 666
BtsCI GGATG 4 cut(s) 447, 1210, 1285, 1507
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 3 cut(s) 681, 865, 1476
BveI ACCTGC 1 cut(s) 1066
Cac8I GCNNGC 2 cut(s) 547, 1231
CfoI GCGC 3 cut(s) 163, 204, 770
Cfr13I GGNCC 3 cut(s) 480, 557, 1191
Csp6I GTAC 3 cut(s) 325, 367, 421
CviQI GTAC 3 cut(s) 325, 367, 421
DdeI CTNAG 2 cut(s) 80, 889
DpnI GATC 9 cut(s) 15, 180, 246, 554, 738, 823, 1017, 1450, 1508
DpnII GATC 9 cut(s) 13, 178, 244, 552, 736, 821, 1015, 1448, 1506
DraI TTTAAA 1 cut(s) 969
DrdI GACNNNNNNGTC 1 cut(s) 172
DseDI GACNNNNNNGTC 1 cut(s) 172
Eam1104I CTCTTC 2 cut(s) 72, 613
EarI CTCTTC 2 cut(s) 72, 613
Ecl136II GAGCTC 1 cut(s) 254
Eco130I CCWWGG 5 cut(s) 74, 724, 1237, 1275, 1497
Eco147I AGGCCT 2 cut(s) 493, 565
Eco24I GRGCYC 2 cut(s) 256, 399
Eco32I GATATC 1 cut(s) 1285
Eco47I GGWCC 1 cut(s) 480
Eco53kI GAGCTC 1 cut(s) 254
Eco57I CTGAAG 2 cut(s) 146, 723
EcoICRI GAGCTC 1 cut(s) 254
EcoNI CCTNNNNNAGG 1 cut(s) 487
EcoO109I RGGNCCY 1 cut(s) 480
EcoRII CCWGG 5 cut(s) 309, 468, 560, 1341, 1530
EcoRV GATATC 1 cut(s) 1285
EcoT14I CCWWGG 5 cut(s) 74, 724, 1237, 1275, 1497
EcoT38I GRGCYC 2 cut(s) 256, 399
ErhI CCWWGG 5 cut(s) 74, 724, 1237, 1275, 1497
FalI AAGNNNNNCTT 4 cut(s) 51, 83, 467, 499
FaqI GGGAC 1 cut(s) 301
FauI CCCGC 1 cut(s) 120
FauNDI CATATG 1 cut(s) 316
FbaI TGATCA 1 cut(s) 1015
FblI GTMKAC 2 cut(s) 678, 1248
Fnu4HI GCNGC 2 cut(s) 649, 804
FokI GGATG 4 cut(s) 454, 1217, 1292, 1514
FriOI GRGCYC 2 cut(s) 256, 399
Fsp4HI GCNGC 2 cut(s) 649, 804
FspBI CTAG 2 cut(s) 591, 1540
FspI TGCGCA 1 cut(s) 162
GlaI GCGC 3 cut(s) 162, 203, 769
GluI GCNGC 2 cut(s) 649, 804
HaeII RGCGCY 1 cut(s) 771
HaeIII GGCC 7 cut(s) 493, 558, 565, 761, 1193, 1475, 1496
HapII CCGG 3 cut(s) 54, 1106, 1119
HhaI GCGC 3 cut(s) 163, 204, 770
Hin1I GRCGYC 1 cut(s) 39
Hin6I GCGC 3 cut(s) 161, 202, 768
HinP1I GCGC 3 cut(s) 161, 202, 768
HinfI GANTC 8 cut(s) 88, 104, 173, 277, 572, 864, 1046, 1457
HpaII CCGG 3 cut(s) 54, 1106, 1119
HphI GGTGA 7 cut(s) 13, 679, 713, 947, 1047, 1121, 1133
Hpy166II GTNNAC 3 cut(s) 679, 856, 1249
Hpy188III TCNNGA 7 cut(s) 286, 591, 615, 788, 825, 1394, 1452
Hpy8I GTNNAC 3 cut(s) 679, 856, 1249
Hpy99I CGWCG 2 cut(s) 41, 186
HpyAV CCTTC 3 cut(s) 121, 443, 1130
HpyCH4III ACNGT 4 cut(s) 661, 853, 860, 1480
HpyCH4IV ACGT 2 cut(s) 39, 167
HpyCH4V TGCA 4 cut(s) 151, 382, 392, 549
HpyF10VI GCNNNNNNNGC 4 cut(s) 555, 713, 767, 1429
HpyF3I CTNAG 2 cut(s) 80, 889
HpySE526I ACGT 2 cut(s) 39, 167
Hsp92I GRCGYC 1 cut(s) 39
HspAI GCGC 3 cut(s) 161, 202, 768
Ksp22I TGATCA 1 cut(s) 1015
Kzo9I GATC 9 cut(s) 13, 178, 244, 552, 736, 821, 1015, 1448, 1506
LmnI GCTCC 3 cut(s) 141, 404, 811
LweI GCATC 2 cut(s) 610, 703
MaeI CTAG 2 cut(s) 591, 1540
MaeII ACGT 2 cut(s) 39, 167
MaeIII GTNAC 4 cut(s) 607, 719, 1022, 1480
MalI GATC 9 cut(s) 15, 180, 246, 554, 738, 823, 1017, 1450, 1508
MboI GATC 9 cut(s) 13, 178, 244, 552, 736, 821, 1015, 1448, 1506
MboII GAAGA 7 cut(s) 59, 373, 630, 722, 831, 853, 1379
MflI RGATCY 3 cut(s) 244, 736, 821
MhlI GDGCHC 2 cut(s) 256, 399
MluCI AATT 3 cut(s) 344, 926, 1313
MlyI GAGTC 2 cut(s) 98, 182
MmeI TCCRAC 3 cut(s) 30, 60, 1061
MseI TTAA 4 cut(s) 221, 918, 968, 1265
MslI CAYNNNNRTG 1 cut(s) 1071
MspI CCGG 3 cut(s) 54, 1106, 1119
MspR9I CCNGG 6 cut(s) 311, 470, 562, 1107, 1343, 1532
Mva1269I GAATGC 1 cut(s) 1530
MvaI CCWGG 5 cut(s) 311, 470, 562, 1343, 1532
MwoI GCNNNNNNNGC 4 cut(s) 555, 713, 767, 1429
NciI CCSGG 1 cut(s) 1107
NcoI CCATGG 5 cut(s) 74, 724, 1237, 1275, 1497
NdeI CATATG 1 cut(s) 316
NdeII GATC 9 cut(s) 13, 178, 244, 552, 736, 821, 1015, 1448, 1506
NlaIV GGNNCC 5 cut(s) 246, 559, 738, 807, 1192
NmeAIII GCCGAG 1 cut(s) 382
NmuCI GTSAC 3 cut(s) 607, 719, 1022
NsbI TGCGCA 1 cut(s) 162
NspI RCATGY 3 cut(s) 584, 784, 1297
NspV TTCGAA 1 cut(s) 91
PaqCI CACCTGC 1 cut(s) 1066
PceI AGGCCT 2 cut(s) 493, 565
PciI ACATGT 3 cut(s) 580, 780, 1293
PcsI WCGNNNNNNNCGW 1 cut(s) 187
PctI GAATGC 1 cut(s) 1530
PfeI GAWTC 6 cut(s) 88, 277, 572, 864, 1046, 1457
Pfl23II CGTACG 1 cut(s) 420
PflMI CCANNNNNTGG 2 cut(s) 730, 1243
PkrI GCNGC 2 cut(s) 650, 805
Ple19I CGATCG 1 cut(s) 181
PleI GAGTC 2 cut(s) 98, 181
PpsI GAGTC 2 cut(s) 98, 181
PpuMI RGGWCCY 1 cut(s) 480
PscI ACATGT 3 cut(s) 580, 780, 1293
Psp124BI GAGCTC 1 cut(s) 256
Psp5II RGGWCCY 1 cut(s) 480
Psp6I CCWGG 5 cut(s) 309, 468, 560, 1341, 1530
PspGI CCWGG 5 cut(s) 309, 468, 560, 1341, 1530
PspLI CGTACG 1 cut(s) 420
PspN4I GGNNCC 5 cut(s) 246, 559, 738, 807, 1192
PspPI GGNCC 3 cut(s) 480, 557, 1191
PspPPI RGGWCCY 1 cut(s) 480
PsrI GAACNNNNNNTAC 2 cut(s) 413, 445
PstI CTGCAG 1 cut(s) 551
PsuI RGATCY 3 cut(s) 244, 736, 821
PvuI CGATCG 1 cut(s) 181
RsaI GTAC 3 cut(s) 326, 368, 422
RsaNI GTAC 3 cut(s) 325, 367, 421
RseI CAYNNNNRTG 1 cut(s) 1071
SacI GAGCTC 1 cut(s) 256
SaqAI TTAA 4 cut(s) 221, 918, 968, 1265
SatI GCNGC 2 cut(s) 649, 804
Sau3AI GATC 9 cut(s) 13, 178, 244, 552, 736, 821, 1015, 1448, 1506
Sau96I GGNCC 3 cut(s) 480, 557, 1191
SbfI CCTGCAGG 1 cut(s) 551
SchI GAGTC 2 cut(s) 98, 182
ScrFI CCNGG 6 cut(s) 311, 470, 562, 1107, 1343, 1532
SdaI CCTGCAGG 1 cut(s) 551
SduI GDGCHC 2 cut(s) 256, 399
SfaNI GCATC 2 cut(s) 610, 703
SfcI CTRYAG 3 cut(s) 547, 567, 795
SfuI TTCGAA 1 cut(s) 91
SinI GGWCC 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 1071
Sse8387I CCTGCAGG 1 cut(s) 551
Sse9I AATT 3 cut(s) 344, 926, 1313
SseBI AGGCCT 2 cut(s) 493, 565
SsiI CCGC 5 cut(s) 113, 648, 707, 803, 1442
SspI AATATT 1 cut(s) 916
SspMI CTAG 2 cut(s) 591, 1540
SstI GAGCTC 1 cut(s) 256
StuI AGGCCT 2 cut(s) 493, 565
StyD4I CCNGG 6 cut(s) 309, 468, 560, 1105, 1341, 1530
StyI CCWWGG 5 cut(s) 74, 724, 1237, 1275, 1497
TaaI ACNGT 4 cut(s) 661, 853, 860, 1480
TaiI ACGT 2 cut(s) 42, 170
TaqI TCGA 9 cut(s) 91, 171, 181, 211, 240, 787, 826, 1221, 1470
TasI AATT 3 cut(s) 344, 926, 1313
TatI WGTACW 1 cut(s) 324
TauI GCSGC 2 cut(s) 651, 806
TfiI GAWTC 6 cut(s) 88, 277, 572, 864, 1046, 1457
Tru1I TTAA 4 cut(s) 221, 918, 968, 1265
Tru9I TTAA 4 cut(s) 221, 918, 968, 1265
TscAI CASTG 3 cut(s) 688, 865, 1483
TseFI GTSAC 3 cut(s) 607, 719, 1022
Tsp45I GTSAC 3 cut(s) 607, 719, 1022
TspDTI ATGAA 4 cut(s) 113, 345, 767, 967
TspGWI ACGGA 2 cut(s) 454, 576
TspRI CASTG 3 cut(s) 688, 865, 1483
Van91I CCANNNNNTGG 2 cut(s) 730, 1243
VpaK11BI GGWCC 1 cut(s) 480
XagI CCTNNNNNAGG 1 cut(s) 487
XapI RAATTY 2 cut(s) 344, 1313
XbaI TCTAGA 1 cut(s) 590
XceI RCATGY 3 cut(s) 584, 784, 1297
XmiI GTMKAC 2 cut(s) 678, 1248
XspI CTAG 2 cut(s) 591, 1540
ZraI GACGTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.