pycom07g20410

Mad3/BUB1 hoMad3/BUB1 homology region 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010450
Physical Location & Seq
Forward (+)
22513988 .. 22516731
2744 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g20410.4

Sequence Viewer

Length: 1620 bp
ATGTTAAAACCTCCCTCCTCCTCCTCCACCTCCACCACCACTACCACCATGTCGAACAACGATCTTTTCACCTCCCTAATCTCCGACGTCAAAACCTACTCCGGCAAAGACCCTCTTCTCCCATGGCTAAGGTACGCTCTCGCCACCTCCATCTCTTCCTCGCTATCAGTTACCATTCTACTGTTTAACCCATTCGATTTCACGTTGTGGTTGCAGAGGGATTCGAAAAATGAACGACTCACTCCCGCCTCATACTCTGAAGGAGAAACTTCCTCGCTTCTTGCAGAAGTGTGCGCAGACGTTCGAATGGATTTTGTCGATGATCCGAGAGCTCTGTTGAGAACAATGGAGGCGAATCAAATCGGGACAAAGCACTCTTTGTTCTACCAGGCATATGCTCTCTACTATGAAAAGAACAAGAAATTTGAGGAAGCTGAGAAGATGTACCACTTGGGAGTGCAAAACCTTGCAGAGCCCATTGGAGCGTTAGAGAAGTCGTACGAGCAGTTCCTTCAACGAATGGAGAGACACAGGAGACACAGAAACCAGGAAAAGAGGACCTCAAAAAGGCCTCTGTCAGATAGGACTATCCCTCCAAATGGAGAAAGGAACAAAGAAAATGCCTGCAGGATCGGCCCCAGGCCTATAGAATCCCAACATGATCCGTGTAGAAGTGAGCATCAAGTGACTTCTGGAAGAGGGTCAGAGGAACCGAAAATGTTTGGCGGCGATGATACAGTTGTGGTGAAGTTTGTAGACACTGCCATTGTTGGAAAGTCTGAAGCGGAAGATGCGTGTCACCATGGATTGGTGGATCCTACTATAAACATGAAAGAGGCCATGAGCGCCATCAATGACATGTTTCGAGAGCCTATAGAGACGGCTCCCATTAGCAGAAGATCTCGACAATCCCAACCGAAGAACAATACTGTGGACAGTGGATTCCAAGTATTTGTTGATGACAACTTAGATAATGGAGGGAAATCAGAAAATAATAAGGGAGATTTGAGTAGAACTGCTACCCACCAGTCTCACCAAGCACCTTTAAACATTTTCATTGATGATGATGATAATGACGACAAAGATAGCCCTGATCAGAGTGACGTCGGAAATCCGACAGATGATTCTACCTCACCTGCATCACATGGAAATGTATTCGTGTTTCCAAATCCAAAAGATTTTCCGGGTGAAAGTTCCGGTGATTTGGATGTTGGAGGCTCATCTCGACCGAGGTTCAGGGAGGATACAATGGTTTGTAGGTTTGTTGGGGCCACCATTTCGGATGAGCCGGAGGAGGTGGAAAATGCCTGCCACCATGGTTTGGTTGACCCTACAATCAACCTAAAGGAAGCCATGGATGATATCAACAACATGTTTGGGAAGCCAATGGAATTTGTAAGAAAGAGGAGAGTGAAAAAGCAGGAAAGAGGACTTGATAGGAAAGAAGATTTTGGTGGGTTTTCTATACTTCCTGATGATAACTTGAACCAGAAGCAAGAGCAGAAAATGCCTGATTTACCGCAGAGATCAAGAGATTCAGATTTGTTCGAGGCCACTGTAACAACAAAGGAGGCCATGGATGAGATCAATAAGATGTTTGGAATGCCCCTGGACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

540

Amino Acids

60.7

Weight (kDa)

5.1

Isoelectric Point (pI)

55.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05510
fragaria_vesca FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900
malus_domestica MD01G1156700.v1.1 MD07G1225400.v1.1
prunus_persica Prupe.2G254200_v2.0.a1
pyrus_communis pycom01g17350 pycom07g20410
rosa_chinensis RchiOBHm_Chr1g0370781
rosa_laevigata RLG00000027010
rosa_multiflora Rmu_sc0009474.1_g000004
rosa_roxburghii Rroxscaffold_4G00286230
rosa_rugosa Rorug01G0359700
rosa_samantha Rh1AG368800 Rh1BG332400 Rh1CG346300 Rh1DG363800
rosa_wichuraiana Rw1G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1144
AatII GACGTC 2 cut(s) 90, 1107
Acc16I TGCGCA 1 cut(s) 295
Acc36I ACCTGC 1 cut(s) 1144
AccB7I CCANNNNNTGG 2 cut(s) 808, 1321
AccI GTMKAC 1 cut(s) 756
AciI CCGC 4 cut(s) 246, 726, 785, 1520
AclWI GGATC 5 cut(s) 317, 638, 656, 809, 822
AcsI RAATTY 2 cut(s) 422, 1391
AcuI CTGAAG 2 cut(s) 279, 801
AcyI GRCGYC 2 cut(s) 87, 1104
AdeI CACNNNGTG 1 cut(s) 207
AfaI GTAC 3 cut(s) 134, 446, 500
AfiI CCNNNNNNNGG 4 cut(s) 567, 599, 808, 1321
AflIII ACRYGT 2 cut(s) 858, 1371
AgsI TTSAA 2 cut(s) 515, 1486
AjnI CCWGG 4 cut(s) 387, 546, 638, 1608
AluBI AGCT 2 cut(s) 332, 434
AluI AGCT 2 cut(s) 332, 434
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 4 cut(s) 520, 529, 872, 1035
AlwI GGATC 5 cut(s) 317, 638, 656, 809, 822
AoxI GGCC 7 cut(s) 569, 634, 641, 837, 1269, 1551, 1572
ApoI RAATTY 2 cut(s) 422, 1391
AspLEI GCGC 2 cut(s) 296, 848
AspS9I GGNCC 3 cut(s) 558, 635, 1269
AsuC2I CCSGG 1 cut(s) 1185
AsuHPI GGTGA 7 cut(s) 61, 757, 791, 1025, 1125, 1199, 1211
AsuII TTCGAA 2 cut(s) 224, 304
AvaII GGWCC 1 cut(s) 558
BaeI ACNNNNGTAYC 4 cut(s) 726, 759, 1236, 1269
BamHI GGATCC 1 cut(s) 814
BanII GRGCYC 2 cut(s) 334, 477
Bbv12I GWGCWC 1 cut(s) 334
BccI CCATC 2 cut(s) 158, 857
BceAI ACGGC 1 cut(s) 897
BciT130I CCWGG 4 cut(s) 389, 548, 640, 1610
BciVI GTATCC 1 cut(s) 1237
BclI TGATCA 1 cut(s) 1093
BcnI CCSGG 1 cut(s) 1185
BcoDI GTCTC 4 cut(s) 520, 529, 872, 1035
BfaI CTAG 1 cut(s) 1618
BfmI CTRYAG 3 cut(s) 625, 645, 873
BfoI RGCGCY 1 cut(s) 849
BfuAI ACCTGC 1 cut(s) 1144
BfuI GTATCC 1 cut(s) 1237
BglII AGATCT 1 cut(s) 899
BisI GCNGC 1 cut(s) 727
BlsI GCNGC 1 cut(s) 728
Bme1390I CCNGG 5 cut(s) 389, 548, 640, 1185, 1610
Bme18I GGWCC 1 cut(s) 558
BmgT120I GGNCC 3 cut(s) 558, 635, 1269
BmiI GGNNCC 5 cut(s) 637, 711, 816, 885, 1270
BmrFI CCNGG 5 cut(s) 389, 548, 640, 1185, 1610
BmsI GCATC 3 cut(s) 688, 781, 1148
Bpu10I CCTNAGC 1 cut(s) 128
Bpu14I TTCGAA 2 cut(s) 224, 304
BpuMI CCSGG 1 cut(s) 1185
BsaHI GRCGYC 2 cut(s) 87, 1104
BsaJI CCNNGG 8 cut(s) 122, 638, 802, 1229, 1315, 1353, 1575, 1608
BsaWI WCCGGW 1 cut(s) 1196
BsaXI ACNNNNNCTCC 2 cut(s) 577, 607
Bsc4I CCNNNNNNNGG 4 cut(s) 567, 599, 808, 1321
Bse1I ACTGG 1 cut(s) 1027
BseBI CCWGG 4 cut(s) 389, 548, 640, 1610
BseDI CCNNGG 8 cut(s) 122, 638, 802, 1229, 1315, 1353, 1575, 1608
BseGI GGATG 4 cut(s) 1213, 1288, 1363, 1585
BseLI CCNNNNNNNGG 4 cut(s) 567, 599, 808, 1321
BseMII CTCAG 1 cut(s) 426
BseNI ACTGG 1 cut(s) 1027
BseRI GAGGAG 5 cut(s) 7, 10, 13, 1307, 1420
Bsh1285I CGRYCG 1 cut(s) 1229
BshFI GGCC 7 cut(s) 571, 636, 643, 839, 1271, 1553, 1574
BsiEI CGRYCG 1 cut(s) 1229
BsiHKAI GWGCWC 1 cut(s) 334
BsiSI CCGG 4 cut(s) 102, 1184, 1197, 1289
BsiWI CGTACG 1 cut(s) 498
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 4 cut(s) 567, 599, 808, 1321
BsmAI GTCTC 4 cut(s) 520, 529, 872, 1035
BsmBI CGTCTC 1 cut(s) 872
BsmFI GGGAC 1 cut(s) 379
BsmI GAATGC 1 cut(s) 1608
BsnI GGCC 7 cut(s) 571, 636, 643, 839, 1271, 1553, 1574
Bsp119I TTCGAA 2 cut(s) 224, 304
Bsp1286I GDGCHC 2 cut(s) 334, 477
Bsp143I GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
Bsp19I CCATGG 5 cut(s) 122, 802, 1315, 1353, 1575
BspACI CCGC 4 cut(s) 246, 726, 785, 1520
BspANI GGCC 7 cut(s) 571, 636, 643, 839, 1271, 1553, 1574
BspCNI CTCAG 1 cut(s) 427
BspLI GGNNCC 5 cut(s) 637, 711, 816, 885, 1270
BspMAI CTGCAG 1 cut(s) 629
BspMI ACCTGC 1 cut(s) 1144
BspPI GGATC 5 cut(s) 317, 638, 656, 809, 822
BspT104I TTCGAA 2 cut(s) 224, 304
BsrI ACTGG 1 cut(s) 1027
BssECI CCNNGG 8 cut(s) 122, 638, 802, 1229, 1315, 1353, 1575, 1608
BssMI GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
BssNI GRCGYC 2 cut(s) 87, 1104
BssT1I CCWWGG 5 cut(s) 122, 802, 1315, 1353, 1575
Bst2UI CCWGG 4 cut(s) 389, 548, 640, 1610
Bst4CI ACNGT 5 cut(s) 183, 739, 931, 938, 1558
Bst6I CTCTTC 3 cut(s) 120, 160, 691
BstACI GRCGYC 2 cut(s) 87, 1104
BstAPI GCANNNNNTGC 1 cut(s) 1507
BstBI TTCGAA 2 cut(s) 224, 304
BstC8I GCNNGC 2 cut(s) 625, 1309
BstDEI CTNAG 3 cut(s) 128, 435, 967
BstDSI CCRYGG 5 cut(s) 122, 802, 1315, 1353, 1575
BstENI CCTNNNNNAGG 1 cut(s) 565
BstF5I GGATG 4 cut(s) 1213, 1288, 1363, 1585
BstH2I RGCGCY 1 cut(s) 849
BstHHI GCGC 2 cut(s) 296, 848
BstKTI GATC 9 cut(s) 64, 325, 633, 664, 817, 902, 1096, 1529, 1587
BstMAI GTCTC 4 cut(s) 520, 529, 872, 1035
BstMBI GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
BstMCI CGRYCG 1 cut(s) 1229
BstMWI GCNNNNNNNGC 4 cut(s) 633, 791, 845, 1507
BstNI CCWGG 4 cut(s) 389, 548, 640, 1610
BstNSI RCATGY 2 cut(s) 862, 1375
BstSCI CCNGG 5 cut(s) 387, 546, 638, 1183, 1608
BstSFI CTRYAG 3 cut(s) 625, 645, 873
BstX2I RGATCY 2 cut(s) 814, 899
BstYI RGATCY 2 cut(s) 814, 899
BsuI GTATCC 1 cut(s) 1237
BsuRI GGCC 7 cut(s) 571, 636, 643, 839, 1271, 1553, 1574
BtgI CCRYGG 5 cut(s) 122, 802, 1315, 1353, 1575
BtgZI GCGATG 1 cut(s) 744
BtsCI GGATG 4 cut(s) 1213, 1288, 1363, 1585
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 3 cut(s) 759, 943, 1554
BveI ACCTGC 1 cut(s) 1144
Cac8I GCNNGC 2 cut(s) 625, 1309
CfoI GCGC 2 cut(s) 296, 848
Cfr13I GGNCC 3 cut(s) 558, 635, 1269
Csp6I GTAC 3 cut(s) 133, 445, 499
CviQI GTAC 3 cut(s) 133, 445, 499
DdeI CTNAG 3 cut(s) 128, 435, 967
DpnI GATC 9 cut(s) 63, 324, 632, 663, 816, 901, 1095, 1528, 1586
DpnII GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
DraI TTTAAA 1 cut(s) 1047
DraIII CACNNNGTG 1 cut(s) 207
Eam1104I CTCTTC 3 cut(s) 120, 160, 691
EarI CTCTTC 3 cut(s) 120, 160, 691
Ecl136II GAGCTC 1 cut(s) 332
Eco130I CCWWGG 5 cut(s) 122, 802, 1315, 1353, 1575
Eco147I AGGCCT 2 cut(s) 571, 643
Eco24I GRGCYC 2 cut(s) 334, 477
Eco32I GATATC 1 cut(s) 1363
Eco47I GGWCC 1 cut(s) 558
Eco53kI GAGCTC 1 cut(s) 332
Eco57I CTGAAG 2 cut(s) 279, 801
EcoICRI GAGCTC 1 cut(s) 332
EcoNI CCTNNNNNAGG 1 cut(s) 565
EcoO109I RGGNCCY 1 cut(s) 558
EcoRII CCWGG 4 cut(s) 387, 546, 638, 1608
EcoRV GATATC 1 cut(s) 1363
EcoT14I CCWWGG 5 cut(s) 122, 802, 1315, 1353, 1575
EcoT38I GRGCYC 2 cut(s) 334, 477
ErhI CCWWGG 5 cut(s) 122, 802, 1315, 1353, 1575
Esp3I CGTCTC 1 cut(s) 872
FalI AAGNNNNNCTT 2 cut(s) 99, 131
FaqI GGGAC 1 cut(s) 379
FauI CCCGC 1 cut(s) 253
FauNDI CATATG 1 cut(s) 394
FbaI TGATCA 1 cut(s) 1093
FblI GTMKAC 1 cut(s) 756
Fnu4HI GCNGC 1 cut(s) 727
FokI GGATG 4 cut(s) 1220, 1295, 1370, 1592
FriOI GRGCYC 2 cut(s) 334, 477
Fsp4HI GCNGC 1 cut(s) 727
FspBI CTAG 1 cut(s) 1618
FspI TGCGCA 1 cut(s) 295
GlaI GCGC 2 cut(s) 295, 847
GluI GCNGC 1 cut(s) 727
HaeII RGCGCY 1 cut(s) 849
HaeIII GGCC 7 cut(s) 571, 636, 643, 839, 1271, 1553, 1574
HapII CCGG 4 cut(s) 102, 1184, 1197, 1289
HhaI GCGC 2 cut(s) 296, 848
Hin1I GRCGYC 2 cut(s) 87, 1104
Hin6I GCGC 2 cut(s) 294, 846
HinP1I GCGC 2 cut(s) 294, 846
HincII GTYRAC 1 cut(s) 1327
HindII GTYRAC 1 cut(s) 1327
HinfI GANTC 7 cut(s) 221, 237, 355, 650, 942, 1124, 1535
HpaII CCGG 4 cut(s) 102, 1184, 1197, 1289
HphI GGTGA 7 cut(s) 61, 757, 791, 1025, 1125, 1199, 1211
Hpy166II GTNNAC 3 cut(s) 757, 934, 1327
Hpy188III TCNNGA 7 cut(s) 364, 693, 866, 903, 1224, 1472, 1530
Hpy8I GTNNAC 3 cut(s) 757, 934, 1327
Hpy99I CGWCG 2 cut(s) 89, 1109
HpyAV CCTTC 2 cut(s) 254, 521
HpyCH4III ACNGT 5 cut(s) 183, 739, 931, 938, 1558
HpyCH4IV ACGT 4 cut(s) 87, 203, 300, 1104
HpyCH4V TGCA 6 cut(s) 214, 284, 460, 470, 627, 1139
HpyF10VI GCNNNNNNNGC 4 cut(s) 633, 791, 845, 1507
HpyF3I CTNAG 3 cut(s) 128, 435, 967
HpySE526I ACGT 4 cut(s) 87, 203, 300, 1104
Hsp92I GRCGYC 2 cut(s) 87, 1104
HspAI GCGC 2 cut(s) 294, 846
Ksp22I TGATCA 1 cut(s) 1093
Kzo9I GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
LmnI GCTCC 2 cut(s) 482, 889
LweI GCATC 3 cut(s) 688, 781, 1148
MaeI CTAG 1 cut(s) 1618
MaeII ACGT 4 cut(s) 87, 203, 300, 1104
MaeIII GTNAC 5 cut(s) 169, 685, 797, 1100, 1558
MalI GATC 9 cut(s) 63, 324, 632, 663, 816, 901, 1095, 1528, 1586
MboI GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
MboII GAAGA 8 cut(s) 107, 147, 451, 708, 800, 909, 931, 1457
MflI RGATCY 2 cut(s) 814, 899
MhlI GDGCHC 2 cut(s) 334, 477
MluCI AATT 2 cut(s) 422, 1391
MlyI GAGTC 1 cut(s) 231
MmeI TCCRAC 5 cut(s) 108, 751, 1087, 1139, 1192
MseI TTAA 3 cut(s) 5, 186, 1046
MslI CAYNNNNRTG 1 cut(s) 1149
MspI CCGG 4 cut(s) 102, 1184, 1197, 1289
MspR9I CCNGG 5 cut(s) 389, 548, 640, 1185, 1610
Mva1269I GAATGC 1 cut(s) 1608
MvaI CCWGG 4 cut(s) 389, 548, 640, 1610
MwoI GCNNNNNNNGC 4 cut(s) 633, 791, 845, 1507
NciI CCSGG 1 cut(s) 1185
NcoI CCATGG 5 cut(s) 122, 802, 1315, 1353, 1575
NdeI CATATG 1 cut(s) 394
NdeII GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
NlaIV GGNNCC 5 cut(s) 637, 711, 816, 885, 1270
NmuCI GTSAC 3 cut(s) 685, 797, 1100
NsbI TGCGCA 1 cut(s) 295
NspI RCATGY 2 cut(s) 862, 1375
NspV TTCGAA 2 cut(s) 224, 304
PaqCI CACCTGC 1 cut(s) 1144
PceI AGGCCT 2 cut(s) 571, 643
PciI ACATGT 2 cut(s) 858, 1371
PctI GAATGC 1 cut(s) 1608
PfeI GAWTC 6 cut(s) 221, 355, 650, 942, 1124, 1535
Pfl23II CGTACG 1 cut(s) 498
PflMI CCANNNNNTGG 2 cut(s) 808, 1321
PkrI GCNGC 1 cut(s) 728
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
PpuMI RGGWCCY 1 cut(s) 558
PscI ACATGT 2 cut(s) 858, 1371
Psp124BI GAGCTC 1 cut(s) 334
Psp5II RGGWCCY 1 cut(s) 558
Psp6I CCWGG 4 cut(s) 387, 546, 638, 1608
PspGI CCWGG 4 cut(s) 387, 546, 638, 1608
PspLI CGTACG 1 cut(s) 498
PspN4I GGNNCC 5 cut(s) 637, 711, 816, 885, 1270
PspPI GGNCC 3 cut(s) 558, 635, 1269
PspPPI RGGWCCY 1 cut(s) 558
PsrI GAACNNNNNNTAC 2 cut(s) 491, 523
PstI CTGCAG 1 cut(s) 629
PsuI RGATCY 2 cut(s) 814, 899
RsaI GTAC 3 cut(s) 134, 446, 500
RsaNI GTAC 3 cut(s) 133, 445, 499
RseI CAYNNNNRTG 1 cut(s) 1149
SacI GAGCTC 1 cut(s) 334
SaqAI TTAA 3 cut(s) 5, 186, 1046
SatI GCNGC 1 cut(s) 727
Sau3AI GATC 9 cut(s) 61, 322, 630, 661, 814, 899, 1093, 1526, 1584
Sau96I GGNCC 3 cut(s) 558, 635, 1269
SbfI CCTGCAGG 1 cut(s) 629
SchI GAGTC 1 cut(s) 231
ScrFI CCNGG 5 cut(s) 389, 548, 640, 1185, 1610
SdaI CCTGCAGG 1 cut(s) 629
SduI GDGCHC 2 cut(s) 334, 477
SfaNI GCATC 3 cut(s) 688, 781, 1148
SfcI CTRYAG 3 cut(s) 625, 645, 873
SfuI TTCGAA 2 cut(s) 224, 304
SinI GGWCC 1 cut(s) 558
SmiMI CAYNNNNRTG 1 cut(s) 1149
Sse8387I CCTGCAGG 1 cut(s) 629
Sse9I AATT 2 cut(s) 422, 1391
SseBI AGGCCT 2 cut(s) 571, 643
SsiI CCGC 4 cut(s) 246, 726, 785, 1520
SspMI CTAG 1 cut(s) 1618
SstI GAGCTC 1 cut(s) 334
StuI AGGCCT 2 cut(s) 571, 643
StyD4I CCNGG 5 cut(s) 387, 546, 638, 1183, 1608
StyI CCWWGG 5 cut(s) 122, 802, 1315, 1353, 1575
TaaI ACNGT 5 cut(s) 183, 739, 931, 938, 1558
TaiI ACGT 4 cut(s) 90, 206, 303, 1107
TaqI TCGA 9 cut(s) 53, 195, 224, 304, 318, 865, 904, 1225, 1548
TaqII GACCGA 1 cut(s) 1243
TasI AATT 2 cut(s) 422, 1391
TauI GCSGC 1 cut(s) 729
TfiI GAWTC 6 cut(s) 221, 355, 650, 942, 1124, 1535
Tru1I TTAA 3 cut(s) 5, 186, 1046
Tru9I TTAA 3 cut(s) 5, 186, 1046
TscAI CASTG 3 cut(s) 766, 943, 1561
TseFI GTSAC 3 cut(s) 685, 797, 1100
Tsp45I GTSAC 3 cut(s) 685, 797, 1100
TspDTI ATGAA 4 cut(s) 246, 423, 845, 1045
TspGWI ACGGA 1 cut(s) 654
TspRI CASTG 3 cut(s) 766, 943, 1561
Van91I CCANNNNNTGG 2 cut(s) 808, 1321
VpaK11BI GGWCC 1 cut(s) 558
XagI CCTNNNNNAGG 1 cut(s) 565
XapI RAATTY 2 cut(s) 422, 1391
XceI RCATGY 2 cut(s) 862, 1375
XmiI GTMKAC 1 cut(s) 756
XspI CTAG 1 cut(s) 1618
ZraI GACGTC 2 cut(s) 88, 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.