MD01G1156700.v1.1

Mad3/BUB1 hoMad3/BUB1 homology region 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010450
Physical Location & Seq
Forward (+)
26415880 .. 26423733
7854 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1156700.v1.1.491

Sequence Viewer

Length: 1500 bp
ATGTCCAACAACGATCTTCTCACCTCCTTAATCTCCGACATCAAAACCTACTCCGGCAAAGACCCTCTTCTTCCATGGCTAAGAGGGATTCGAAAAATGAACGACTCACTCCCACCTCGCACTCTGAAGGAGAAGCTCCCTCGCTTCTTGCAGAAATGCGCGCAGACATTCGAGTCTGATCGGCGATACCGAAAAGACTTGCGCTACATTCGAGTTTGGTTGAAGTTGATGGATTTTGTCGATGATCCAAGAGCTTTGTTGAGAACAATGGTGGTGAATCAAATCGGGACAAAGCACTCCTTGTTCTACCGGGCATATGCTCTCTACTATGAAAAGAACAAGAAATTTGAGGAGGCTGAGAAGATGTACCACTTGGGAGTGCAAAACCTTGCAGAGCCCATTGGAGCGTTAGAGAAGTCATACGAGCAGTTCCTTCAACGCATGGAGAGACACAGGAGACACAGAAACCAGAAAATTGTTGTGCATCGCCAGGAAAAGAGGACGACCACGAAAAGGCCTCTGTTGGATCGGACTATCCCTCCAAATGGAGAAAGAAGCAAAGAAAATGCCTGCAGAATCGGGGCCAGGCCTATAGAATCCCAACATGATCCGTCTAGAAGTAAGCGTCAAGTGACTTCTGGAAGAGGGTCAGAGGAACCGAAGATGTTTGGCGGCGATGATACAGTTGTGGTGAAGTTTGTAGACACTGCCATTGTTGGAAAGTCTGAAGCGGAAGATGCGTGTCATCATGGATTGGTGGATCCTACTATAAACATGAAAGAGGCCATGAGCGCCATCAACGACATGTTTCGAGAGCCTATAGAAACGGCTCCCATTAGTAGAAGACCTCGCCAATCCCAACCAAAGAACAATAATGTGGACATGGATGCCAGGTATTTGTTGATGACGACTTGGATAATGGAGGGAAATCAGAAAATAATAAGGAAGATGAGTAGAACTGCTACCCAACAGTCTCACCAAGAACCTTTAAACATTTTCATTGATGATGATAACGATAGCCCTGATCAGAGTGGCGTCCAAAATCCAACAGATGATTCTACCTCACCAGCTTCACATAGAAATGTGTTTGTGTTTCCAAAACCGAAGGATCTTCCAGGTGAAAGTTCCAGTGATTTGGACGTTGGAGGCTCATCTCGACCGAGGTTCAGGGAGGATACAATGGTTTGTAGGTTTGTTGGGGCCACCATTTCGGATGAGCCAGAGGAGGTCGAAAATGCTTGCCACCATGGTTTAGTCGACCCGACAATAAACTTAAAAGAGGCCATGGATGATATCAATAACATGTTCGGGAAGCCTATGGATTTTGTAAGAAAGAGGAGAGTGAAGAAGCAGGACAGAGGAATTGATAGGAAAGAAGATTTTGGTGGGTTTTCTATACTTCCTGATGATAACTTGGACCAAAATGCTAGATTTGTCGGGAGGATCAAGAGGTTCGGATTTGTTCGAGGCCACTGTAACAACAAAGGAGGCCATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

500

Amino Acids

57.43

Weight (kDa)

9.26

Isoelectric Point (pI)

51.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 10 - 127 8.8e-36 Mad3/BUB1 homology region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05510
fragaria_vesca FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900 FvH4_7g24900
malus_domestica MD01G1156700.v1.1 MD07G1225400.v1.1
prunus_persica Prupe.2G254200_v2.0.a1
pyrus_communis pycom01g17350 pycom07g20410
rosa_chinensis RchiOBHm_Chr1g0370781
rosa_laevigata RLG00000027010
rosa_multiflora Rmu_sc0009474.1_g000004
rosa_roxburghii Rroxscaffold_4G00286230
rosa_rugosa Rorug01G0359700
rosa_samantha Rh1AG368800 Rh1BG332400 Rh1CG346300 Rh1DG363800
rosa_wichuraiana Rw1G032470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 172
AccI GTMKAC 2 cut(s) 702, 1257
AccII CGCG 1 cut(s) 161
AciI CCGC 2 cut(s) 672, 731
AclWI GGATC 7 cut(s) 239, 534, 602, 755, 768, 1116, 1451
AcsI RAATTY 1 cut(s) 344
AcuI CTGAAG 2 cut(s) 146, 747
AcyI GRCGYC 1 cut(s) 1035
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 2 cut(s) 513, 545
AflIII ACRYGT 2 cut(s) 804, 1302
AgsI TTSAA 2 cut(s) 223, 437
AjnI CCWGG 4 cut(s) 489, 584, 890, 1114
AjuI GAANNNNNNNTTGG 2 cut(s) 1039, 1071
AluBI AGCT 3 cut(s) 136, 254, 1070
AluI AGCT 3 cut(s) 136, 254, 1070
Alw26I GTCTC 3 cut(s) 442, 451, 978
AlwI GGATC 7 cut(s) 239, 534, 602, 755, 768, 1116, 1451
AoxI GGCC 8 cut(s) 515, 582, 587, 783, 1200, 1281, 1468, 1489
ApoI RAATTY 1 cut(s) 344
AspLEI GCGC 4 cut(s) 161, 163, 204, 794
AspS9I GGNCC 3 cut(s) 582, 1200, 1417
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 6 cut(s) 13, 286, 703, 968, 1056, 1130
AsuII TTCGAA 1 cut(s) 91
AvaII GGWCC 1 cut(s) 1417
BaeI ACNNNNGTAYC 4 cut(s) 672, 705, 1167, 1200
BamHI GGATCC 1 cut(s) 760
BanII GRGCYC 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 850
BccI CCATC 2 cut(s) 223, 803
BceAI ACGGC 1 cut(s) 843
BciT130I CCWGG 4 cut(s) 491, 586, 892, 1116
BciVI GTATCC 1 cut(s) 1168
BclI TGATCA 1 cut(s) 1024
BcnI CCSGG 1 cut(s) 311
BcoDI GTCTC 3 cut(s) 442, 451, 978
BfaI CTAG 2 cut(s) 615, 1428
BfmI CTRYAG 3 cut(s) 571, 591, 819
BfoI RGCGCY 1 cut(s) 795
BfuI GTATCC 1 cut(s) 1168
BisI GCNGC 1 cut(s) 673
BlsI GCNGC 1 cut(s) 674
Bme1390I CCNGG 5 cut(s) 311, 491, 586, 892, 1116
Bme18I GGWCC 1 cut(s) 1417
BmgT120I GGNCC 3 cut(s) 582, 1200, 1417
BmiI GGNNCC 5 cut(s) 583, 657, 762, 831, 1201
BmrFI CCNGG 5 cut(s) 311, 491, 586, 892, 1116
BmsI GCATC 3 cut(s) 493, 727, 877
BpiI GAAGAC 1 cut(s) 850
Bpu14I TTCGAA 1 cut(s) 91
BpuMI CCSGG 1 cut(s) 311
BsaHI GRCGYC 1 cut(s) 1035
BsaJI CCNNGG 5 cut(s) 74, 1160, 1246, 1284, 1492
BsaXI ACNNNNNCTCC 2 cut(s) 523, 553
Bsc4I CCNNNNNNNGG 2 cut(s) 513, 545
Bse1I ACTGG 1 cut(s) 1128
BseBI CCWGG 4 cut(s) 491, 586, 892, 1116
BseDI CCNNGG 5 cut(s) 74, 1160, 1246, 1284, 1492
BseGI GGATG 3 cut(s) 892, 1219, 1294
BseLI CCNNNNNNNGG 2 cut(s) 513, 545
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 1 cut(s) 1128
BsePI GCGCGC 1 cut(s) 159
BseRI GAGGAG 3 cut(s) 365, 1238, 1351
Bsh1236I CGCG 1 cut(s) 161
Bsh1285I CGRYCG 1 cut(s) 1160
BshFI GGCC 8 cut(s) 517, 584, 589, 785, 1202, 1283, 1470, 1491
BsiEI CGRYCG 1 cut(s) 1160
BsiSI CCGG 2 cut(s) 54, 310
BslFI GGGAC 1 cut(s) 301
BslI CCNNNNNNNGG 2 cut(s) 513, 545
BsmAI GTCTC 3 cut(s) 442, 451, 978
BsmFI GGGAC 1 cut(s) 301
BsnI GGCC 8 cut(s) 517, 584, 589, 785, 1202, 1283, 1470, 1491
Bsp119I TTCGAA 1 cut(s) 91
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 9 cut(s) 13, 178, 244, 526, 607, 760, 1024, 1108, 1443
Bsp19I CCATGG 4 cut(s) 74, 1246, 1284, 1492
BspACI CCGC 2 cut(s) 672, 731
BspANI GGCC 8 cut(s) 517, 584, 589, 785, 1202, 1283, 1470, 1491
BspCNI CTCAG 1 cut(s) 349
BspFNI CGCG 1 cut(s) 161
BspLI GGNNCC 5 cut(s) 583, 657, 762, 831, 1201
BspMAI CTGCAG 1 cut(s) 575
BspPI GGATC 7 cut(s) 239, 534, 602, 755, 768, 1116, 1451
BspT104I TTCGAA 1 cut(s) 91
BsrI ACTGG 1 cut(s) 1128
BssECI CCNNGG 5 cut(s) 74, 1160, 1246, 1284, 1492
BssHII GCGCGC 1 cut(s) 159
BssMI GATC 9 cut(s) 13, 178, 244, 526, 607, 760, 1024, 1108, 1443
BssNI GRCGYC 1 cut(s) 1035
BssT1I CCWWGG 4 cut(s) 74, 1246, 1284, 1492
Bst2UI CCWGG 4 cut(s) 491, 586, 892, 1116
Bst4CI ACNGT 3 cut(s) 685, 972, 1475
Bst6I CTCTTC 2 cut(s) 72, 637
BstACI GRCGYC 1 cut(s) 1035
BstBI TTCGAA 1 cut(s) 91
BstC8I GCNNGC 3 cut(s) 161, 571, 1240
BstDEI CTNAG 2 cut(s) 80, 357
BstDSI CCRYGG 4 cut(s) 74, 1246, 1284, 1492
BstF5I GGATG 3 cut(s) 892, 1219, 1294
BstFNI CGCG 1 cut(s) 161
BstH2I RGCGCY 1 cut(s) 795
BstHHI GCGC 4 cut(s) 161, 163, 204, 794
BstKTI GATC 9 cut(s) 16, 181, 247, 529, 610, 763, 1027, 1111, 1446
BstMAI GTCTC 3 cut(s) 442, 451, 978
BstMBI GATC 9 cut(s) 13, 178, 244, 526, 607, 760, 1024, 1108, 1443
BstMCI CGRYCG 1 cut(s) 1160
BstMWI GCNNNNNNNGC 2 cut(s) 737, 791
BstNI CCWGG 4 cut(s) 491, 586, 892, 1116
BstNSI RCATGY 2 cut(s) 808, 1306
BstSCI CCNGG 5 cut(s) 309, 489, 584, 890, 1114
BstSFI CTRYAG 3 cut(s) 571, 591, 819
BstUI CGCG 1 cut(s) 161
BstV2I GAAGAC 1 cut(s) 850
BstX2I RGATCY 2 cut(s) 760, 1108
BstXI CCANNNNNNTGG 1 cut(s) 1135
BstYI RGATCY 2 cut(s) 760, 1108
BsuI GTATCC 1 cut(s) 1168
BsuRI GGCC 8 cut(s) 517, 584, 589, 785, 1202, 1283, 1470, 1491
BtgI CCRYGG 4 cut(s) 74, 1246, 1284, 1492
BtgZI GCGATG 2 cut(s) 470, 690
BtsCI GGATG 3 cut(s) 892, 1219, 1294
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 3 cut(s) 705, 1135, 1471
Cac8I GCNNGC 3 cut(s) 161, 571, 1240
CfoI GCGC 4 cut(s) 161, 163, 204, 794
Cfr13I GGNCC 3 cut(s) 582, 1200, 1417
CseI GACGC 2 cut(s) 614, 1024
Csp6I GTAC 1 cut(s) 367
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 2 cut(s) 80, 357
DpnI GATC 9 cut(s) 15, 180, 246, 528, 609, 762, 1026, 1110, 1445
DpnII GATC 9 cut(s) 13, 178, 244, 526, 607, 760, 1024, 1108, 1443
DraI TTTAAA 1 cut(s) 990
DrdI GACNNNNNNGTC 1 cut(s) 172
DseDI GACNNNNNNGTC 1 cut(s) 172
Eam1104I CTCTTC 2 cut(s) 72, 637
EarI CTCTTC 2 cut(s) 72, 637
Eco130I CCWWGG 4 cut(s) 74, 1246, 1284, 1492
Eco147I AGGCCT 2 cut(s) 517, 589
Eco24I GRGCYC 1 cut(s) 399
Eco32I GATATC 1 cut(s) 1294
Eco47I GGWCC 1 cut(s) 1417
Eco57I CTGAAG 2 cut(s) 146, 747
EcoRII CCWGG 4 cut(s) 489, 584, 890, 1114
EcoRV GATATC 1 cut(s) 1294
EcoT14I CCWWGG 4 cut(s) 74, 1246, 1284, 1492
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 4 cut(s) 74, 1246, 1284, 1492
FalI AAGNNNNNCTT 4 cut(s) 51, 83, 284, 316
FaqI GGGAC 1 cut(s) 301
FauNDI CATATG 1 cut(s) 316
FbaI TGATCA 1 cut(s) 1024
FblI GTMKAC 2 cut(s) 702, 1257
Fnu4HI GCNGC 1 cut(s) 673
FokI GGATG 3 cut(s) 899, 1226, 1301
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 1 cut(s) 673
FspBI CTAG 2 cut(s) 615, 1428
GlaI GCGC 4 cut(s) 160, 162, 203, 793
GluI GCNGC 1 cut(s) 673
HaeII RGCGCY 1 cut(s) 795
HaeIII GGCC 8 cut(s) 517, 584, 589, 785, 1202, 1283, 1470, 1491
HapII CCGG 2 cut(s) 54, 310
HgaI GACGC 2 cut(s) 614, 1024
HhaI GCGC 4 cut(s) 161, 163, 204, 794
Hin1I GRCGYC 1 cut(s) 1035
Hin6I GCGC 4 cut(s) 159, 161, 202, 792
HinP1I GCGC 4 cut(s) 159, 161, 202, 792
HincII GTYRAC 1 cut(s) 1258
HindII GTYRAC 1 cut(s) 1258
HinfI GANTC 7 cut(s) 88, 104, 173, 277, 576, 596, 1055
HpaII CCGG 2 cut(s) 54, 310
HphI GGTGA 6 cut(s) 13, 286, 703, 968, 1056, 1130
Hpy166II GTNNAC 3 cut(s) 703, 880, 1258
Hpy188III TCNNGA 9 cut(s) 286, 615, 639, 812, 1155, 1309, 1403, 1438, 1447
Hpy8I GTNNAC 3 cut(s) 703, 880, 1258
HpyAV CCTTC 3 cut(s) 121, 443, 1099
HpyCH4III ACNGT 3 cut(s) 685, 972, 1475
HpyCH4IV ACGT 1 cut(s) 1140
HpyCH4V TGCA 5 cut(s) 151, 382, 392, 484, 573
HpyF10VI GCNNNNNNNGC 2 cut(s) 737, 791
HpyF3I CTNAG 2 cut(s) 80, 357
HpySE526I ACGT 1 cut(s) 1140
Hsp92I GRCGYC 1 cut(s) 1035
HspAI GCGC 4 cut(s) 159, 161, 202, 792
Ksp22I TGATCA 1 cut(s) 1024
Kzo9I GATC 9 cut(s) 13, 178, 244, 526, 607, 760, 1024, 1108, 1443
LmnI GCTCC 3 cut(s) 141, 404, 835
LweI GCATC 3 cut(s) 493, 727, 877
MaeI CTAG 2 cut(s) 615, 1428
MaeII ACGT 1 cut(s) 1140
MaeIII GTNAC 2 cut(s) 631, 1475
MalI GATC 9 cut(s) 15, 180, 246, 528, 609, 762, 1026, 1110, 1445
MboI GATC 9 cut(s) 13, 178, 244, 526, 607, 760, 1024, 1108, 1443
MflI RGATCY 2 cut(s) 760, 1108
MhlI GDGCHC 1 cut(s) 399
MluCI AATT 3 cut(s) 344, 474, 1362
MlyI GAGTC 2 cut(s) 98, 182
MmeI TCCRAC 6 cut(s) 30, 60, 504, 697, 1070, 1123
MseI TTAA 3 cut(s) 29, 989, 1274
MslI CAYNNNNRTG 1 cut(s) 1080
MspI CCGG 2 cut(s) 54, 310
MspR9I CCNGG 5 cut(s) 311, 491, 586, 892, 1116
MvaI CCWGG 4 cut(s) 491, 586, 892, 1116
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 2 cut(s) 737, 791
NciI CCSGG 1 cut(s) 311
NcoI CCATGG 4 cut(s) 74, 1246, 1284, 1492
NdeI CATATG 1 cut(s) 316
NdeII GATC 9 cut(s) 13, 178, 244, 526, 607, 760, 1024, 1108, 1443
NlaIV GGNNCC 5 cut(s) 583, 657, 762, 831, 1201
NmuCI GTSAC 1 cut(s) 631
NspI RCATGY 2 cut(s) 808, 1306
NspV TTCGAA 1 cut(s) 91
PauI GCGCGC 1 cut(s) 159
PceI AGGCCT 2 cut(s) 517, 589
PciI ACATGT 2 cut(s) 804, 1302
PcsI WCGNNNNNNNCGW 1 cut(s) 187
PfeI GAWTC 5 cut(s) 88, 277, 576, 596, 1055
PkrI GCNGC 1 cut(s) 674
PleI GAGTC 2 cut(s) 98, 181
PpsI GAGTC 2 cut(s) 98, 181
PscI ACATGT 2 cut(s) 804, 1302
Psp6I CCWGG 4 cut(s) 489, 584, 890, 1114
PspGI CCWGG 4 cut(s) 489, 584, 890, 1114
PspN4I GGNNCC 5 cut(s) 583, 657, 762, 831, 1201
PspPI GGNCC 3 cut(s) 582, 1200, 1417
PstI CTGCAG 1 cut(s) 575
PsuI RGATCY 2 cut(s) 760, 1108
PteI GCGCGC 1 cut(s) 159
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
RseI CAYNNNNRTG 1 cut(s) 1080
SalI GTCGAC 1 cut(s) 1256
SaqAI TTAA 3 cut(s) 29, 989, 1274
SatI GCNGC 1 cut(s) 673
Sau3AI GATC 9 cut(s) 13, 178, 244, 526, 607, 760, 1024, 1108, 1443
Sau96I GGNCC 3 cut(s) 582, 1200, 1417
SchI GAGTC 2 cut(s) 98, 182
ScrFI CCNGG 5 cut(s) 311, 491, 586, 892, 1116
SduI GDGCHC 1 cut(s) 399
SfaNI GCATC 3 cut(s) 493, 727, 877
SfcI CTRYAG 3 cut(s) 571, 591, 819
SfuI TTCGAA 1 cut(s) 91
SinI GGWCC 1 cut(s) 1417
SmiMI CAYNNNNRTG 1 cut(s) 1080
Sse9I AATT 3 cut(s) 344, 474, 1362
SseBI AGGCCT 2 cut(s) 517, 589
SsiI CCGC 2 cut(s) 672, 731
SspMI CTAG 2 cut(s) 615, 1428
StuI AGGCCT 2 cut(s) 517, 589
StyD4I CCNGG 5 cut(s) 309, 489, 584, 890, 1114
StyI CCWWGG 4 cut(s) 74, 1246, 1284, 1492
TaaI ACNGT 3 cut(s) 685, 972, 1475
TaiI ACGT 1 cut(s) 1143
TaqI TCGA 9 cut(s) 91, 171, 211, 240, 811, 1156, 1230, 1257, 1465
TaqII GACCGA 1 cut(s) 1174
TasI AATT 3 cut(s) 344, 474, 1362
TauI GCSGC 1 cut(s) 675
TfiI GAWTC 5 cut(s) 88, 277, 576, 596, 1055
Tru1I TTAA 3 cut(s) 29, 989, 1274
Tru9I TTAA 3 cut(s) 29, 989, 1274
TscAI CASTG 3 cut(s) 712, 1135, 1478
TseFI GTSAC 1 cut(s) 631
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 4 cut(s) 113, 345, 791, 988
TspGWI ACGGA 1 cut(s) 600
TspRI CASTG 3 cut(s) 712, 1135, 1478
VpaK11BI GGWCC 1 cut(s) 1417
XapI RAATTY 1 cut(s) 344
XbaI TCTAGA 1 cut(s) 614
XceI RCATGY 2 cut(s) 808, 1306
XmiI GTMKAC 2 cut(s) 702, 1257
XspI CTAG 2 cut(s) 615, 1428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.