FvH4_7g26311

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014972
Physical Location & Seq
Reverse (-)
19916040 .. 19917201
1162 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g26311.t1

Sequence Viewer

Length: 1017 bp
ATGGGGAAACTCTGGGTTGAAGTTTGCTTGATATCTGCACGAGGGCTTCGACGTTCATCGTCCCTGTGGAAGCTTCAATGGTATGCTGTGGGGTGGACTAATCCCAATAACAAGTACTGCACCAAGATTGATGCATCTGGAAATGCAAATCCTGTATGGAAAACCAAGTTTGCTACCTTGGTTGAGGACGCGGAATCTAAACTGAATGATTTGGCTCTGCACATTGAAGTCTATAGTAGAGAGCCTATATTCCTCAGAGAAAGGCTGCAGGGGACAGCTACTATTCTGTTGAAAGAGTTTCTGGCTAAGCATAACAAGAATTCTGAGAATTCAAGGCATGGAGCTGAAGAAGTTGGGAGCTACCAATTGCGAAAAAAGAATTCCAACAAACCTCAAGGTTTTGTTGATGTTTCAATCCACATTTCTGAAGATATGGAAGGGAGGAGCTCATACACAGGAAATGAGGGAGGACCTGGGGAGTACAGCAATACTATCACCTTGGCTACCGGAGATGGGTCTACACCAGCATTTCTACCTTTTACTCCACAAAAGAGACCAGAACATCAGTTCCAAATGAATTCTCCGTATACAAATGCAATGCCTCCGACTACAAACTATTCAAAACCATCTGCATATGGACCAAGCTACACACCAGCTAGCGGAACAAGCTACCCGCCAGCAACCGGACAGAGCTACCCGCCAGCAAGTGGACCAAGCTATCAGCCATCTAGAACTCCTCCACCGCCTCCACCACCTTCCAACGTGGGGTATATACCTACTTTTCTCCCAAGGACAGACCAAATGCCTGAGAGCTATATCAATATGCCATCATCATCTGGAGCACCACATCCTGGTCGTGGAACTCGAGCTGGAGGACCTGGTTTTGCAATGGGAATGGGTGCTGGGGCCCTGGCAGCCGGTGCTGTGATCTTCGGTGATGACTTTATGTCAGGATTTGATGTTCCTTCAGGCTTACAGAATGCTAGTGTTACTATATCGGCTGATCCTCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

339

Amino Acids

36.4

Weight (kDa)

8.37

Isoelectric Point (pI)

57.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 106 2.1e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40815 AT2G40815
fragaria_vesca FvH4_7g26311
malus_domestica MD01G1172000.v1.1 MD07G1238500.v1.1
prunus_persica Prupe.2G267300_v2.0.a1
pyrus_communis pycom07g21450
rosa_chinensis RchiOBHm_Chr1g0372771
rosa_laevigata RLG00000026873
rosa_multiflora Rmu_sc0029212.1_g000001
rosa_rugosa Rorug01G0042900 Rorug01G0374300
rosa_samantha Rh1AG383000 Rh1CG359900 Rh1DG377600
rosa_wichuraiana Rw1G033710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 707, 851
AccI GTMKAC 2 cut(s) 518, 587
AccII CGCG 1 cut(s) 191
AciI CCGC 5 cut(s) 191, 660, 674, 698, 743
AclWI GGATC 1 cut(s) 998
AcsI RAATTY 4 cut(s) 319, 328, 379, 577
AcuI CTGAAG 3 cut(s) 366, 447, 951
AfaI GTAC 2 cut(s) 116, 482
AfiI CCNNNNNNNGG 7 cut(s) 513, 659, 683, 707, 765, 851, 857
AgsI TTSAA 7 cut(s) 20, 77, 227, 292, 333, 414, 621
AhdI GACNNNNNGTC 1 cut(s) 946
AjnI CCWGG 4 cut(s) 472, 850, 877, 909
AloI GAACNNNNNNTCC 4 cut(s) 552, 584, 945, 977
Alw21I GWGCWC 2 cut(s) 449, 844
Alw26I GTCTC 1 cut(s) 547
AlwI GGATC 1 cut(s) 998
Ama87I CYCGRG 1 cut(s) 864
AoxI GGCC 1 cut(s) 906
ApaI GGGCCC 1 cut(s) 910
ApeKI GCWGC 2 cut(s) 265, 914
ApoI RAATTY 4 cut(s) 319, 328, 379, 577
AspS9I GGNCC 6 cut(s) 470, 638, 710, 875, 906, 907
AsuHPI GGTGA 2 cut(s) 487, 947
AsuNHI GCTAGC 1 cut(s) 656
AvaI CYCGRG 1 cut(s) 864
AvaII GGWCC 4 cut(s) 470, 638, 710, 875
BaeGI GKGCMC 1 cut(s) 910
BanII GRGCYC 2 cut(s) 449, 910
BauI CACGAG 1 cut(s) 39
Bbv12I GWGCWC 2 cut(s) 449, 844
BbvI GCAGC 2 cut(s) 252, 926
BccI CCATC 4 cut(s) 506, 634, 733, 835
BciT130I CCWGG 4 cut(s) 474, 852, 879, 911
BcoDI GTCTC 1 cut(s) 547
BfaI CTAG 3 cut(s) 657, 729, 984
BfmI CTRYAG 2 cut(s) 232, 266
BisI GCNGC 2 cut(s) 266, 915
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 2 cut(s) 267, 916
BmcAI AGTACT 1 cut(s) 116
Bme1390I CCNGG 4 cut(s) 474, 852, 879, 911
Bme18I GGWCC 4 cut(s) 470, 638, 710, 875
BmeRI GACNNNNNGTC 1 cut(s) 946
BmeT110I CYCGRG 1 cut(s) 864
BmgT120I GGNCC 6 cut(s) 470, 638, 710, 875, 906, 907
BmiI GGNNCC 2 cut(s) 907, 908
BmrFI CCNGG 4 cut(s) 474, 852, 879, 911
BmsI GCATC 2 cut(s) 121, 143
BmtI GCTAGC 1 cut(s) 660
BpmI CTGGAG 2 cut(s) 858, 891
Bpu1102I GCTNAGC 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 378
BsaI GGTCTC 1 cut(s) 547
BsaJI CCNNGG 5 cut(s) 177, 473, 498, 788, 909
BsaWI WCCGGW 2 cut(s) 506, 683
Bsc4I CCNNNNNNNGG 7 cut(s) 513, 659, 683, 707, 765, 851, 857
Bse118I RCCGGY 1 cut(s) 917
Bse3DI GCAATG 2 cut(s) 603, 894
BseBI CCWGG 4 cut(s) 474, 852, 879, 911
BseDI CCNNGG 5 cut(s) 177, 473, 498, 788, 909
BseGI GGATG 1 cut(s) 847
BseLI CCNNNNNNNGG 7 cut(s) 513, 659, 683, 707, 765, 851, 857
BseMI GCAATG 2 cut(s) 603, 894
BseMII CTCAG 3 cut(s) 268, 315, 798
BseRI GAGGAG 2 cut(s) 457, 726
BseSI GKGCMC 1 cut(s) 910
BseXI GCAGC 2 cut(s) 252, 926
BseYI CCCAGC 1 cut(s) 902
BsgI GTGCAG 3 cut(s) 21, 103, 203
Bsh1236I CGCG 1 cut(s) 191
BshFI GGCC 1 cut(s) 908
BsiHKAI GWGCWC 2 cut(s) 449, 844
BsiHKCI CYCGRG 1 cut(s) 864
BsiSI CCGG 3 cut(s) 507, 684, 918
BslFI GGGAC 2 cut(s) 46, 286
BslI CCNNNNNNNGG 7 cut(s) 513, 659, 683, 707, 765, 851, 857
BsmAI GTCTC 1 cut(s) 547
BsmFI GGGAC 2 cut(s) 46, 286
BsmI GAATGC 1 cut(s) 985
BsnI GGCC 1 cut(s) 908
Bso31I GGTCTC 1 cut(s) 547
BsoBI CYCGRG 1 cut(s) 864
Bsp120I GGGCCC 1 cut(s) 906
Bsp1286I GDGCHC 3 cut(s) 449, 844, 910
Bsp143I GATC 2 cut(s) 927, 1003
Bsp1720I GCTNAGC 1 cut(s) 306
BspACI CCGC 5 cut(s) 191, 660, 674, 698, 743
BspANI GGCC 1 cut(s) 908
BspCNI CTCAG 3 cut(s) 267, 316, 799
BspFNI CGCG 1 cut(s) 191
BspLI GGNNCC 2 cut(s) 907, 908
BspMAI CTGCAG 1 cut(s) 270
BspOI GCTAGC 1 cut(s) 660
BspPI GGATC 1 cut(s) 998
BspTNI GGTCTC 1 cut(s) 547
BsrDI GCAATG 2 cut(s) 603, 894
BsrFI RCCGGY 1 cut(s) 917
BssAI RCCGGY 1 cut(s) 917
BssECI CCNNGG 5 cut(s) 177, 473, 498, 788, 909
BssMI GATC 2 cut(s) 927, 1003
BssNAI GTATAC 1 cut(s) 588
BssSI CACGAG 1 cut(s) 39
BssT1I CCWWGG 3 cut(s) 177, 498, 788
Bst1107I GTATAC 1 cut(s) 588
Bst2BI CACGAG 1 cut(s) 39
Bst2UI CCWGG 4 cut(s) 474, 852, 879, 911
BstAPI GCANNNNNTGC 1 cut(s) 920
BstC8I GCNNGC 3 cut(s) 658, 678, 702
BstDEI CTNAG 4 cut(s) 254, 306, 324, 807
BstF5I GGATG 1 cut(s) 847
BstFNI CGCG 1 cut(s) 191
BstKTI GATC 2 cut(s) 930, 1006
BstMAI GTCTC 1 cut(s) 547
BstMBI GATC 2 cut(s) 927, 1003
BstMWI GCNNNNNNNGC 3 cut(s) 666, 914, 920
BstNI CCWGG 4 cut(s) 474, 852, 879, 911
BstSCI CCNGG 4 cut(s) 472, 850, 877, 909
BstSFI CTRYAG 2 cut(s) 232, 266
BstSLI GKGCMC 1 cut(s) 910
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 2 cut(s) 252, 926
BstZ17I GTATAC 1 cut(s) 588
BsuRI GGCC 1 cut(s) 908
BtsCI GGATG 1 cut(s) 847
Cac8I GCNNGC 3 cut(s) 658, 678, 702
Cfr10I RCCGGY 1 cut(s) 917
Cfr13I GGNCC 6 cut(s) 470, 638, 710, 875, 906, 907
CseI GACGC 1 cut(s) 197
CsiI ACCWGGT 1 cut(s) 877
Csp6I GTAC 2 cut(s) 115, 481
CviAII CATG 1 cut(s) 338
CviQI GTAC 2 cut(s) 115, 481
DdeI CTNAG 4 cut(s) 254, 306, 324, 807
DpnI GATC 2 cut(s) 929, 1005
DpnII GATC 2 cut(s) 927, 1003
DriI GACNNNNNGTC 1 cut(s) 946
Eam1105I GACNNNNNGTC 1 cut(s) 946
Ecl136II GAGCTC 1 cut(s) 447
Eco130I CCWWGG 3 cut(s) 177, 498, 788
Eco24I GRGCYC 2 cut(s) 449, 910
Eco31I GGTCTC 1 cut(s) 547
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 4 cut(s) 470, 638, 710, 875
Eco53kI GAGCTC 1 cut(s) 447
Eco57I CTGAAG 3 cut(s) 366, 447, 951
Eco88I CYCGRG 1 cut(s) 864
EcoICRI GAGCTC 1 cut(s) 447
EcoO109I RGGNCCY 4 cut(s) 470, 875, 906, 907
EcoRI GAATTC 4 cut(s) 319, 328, 379, 577
EcoRII CCWGG 4 cut(s) 472, 850, 877, 909
EcoRV GATATC 1 cut(s) 33
EcoT14I CCWWGG 3 cut(s) 177, 498, 788
EcoT22I ATGCAT 1 cut(s) 136
EcoT38I GRGCYC 2 cut(s) 449, 910
ErhI CCWWGG 3 cut(s) 177, 498, 788
FaeI CATG 1 cut(s) 341
FaqI GGGAC 2 cut(s) 46, 286
FatI CATG 1 cut(s) 337
FauI CCCGC 2 cut(s) 681, 705
FauNDI CATATG 1 cut(s) 634
FblI GTMKAC 2 cut(s) 518, 587
Fnu4HI GCNGC 2 cut(s) 266, 915
FokI GGATG 1 cut(s) 834
FriOI GRGCYC 2 cut(s) 449, 910
Fsp4HI GCNGC 2 cut(s) 266, 915
FspBI CTAG 3 cut(s) 657, 729, 984
GluI GCNGC 2 cut(s) 266, 915
GsaI CCCAGC 1 cut(s) 906
GsuI CTGGAG 2 cut(s) 858, 891
HaeIII GGCC 1 cut(s) 908
HapII CCGG 3 cut(s) 507, 684, 918
HgaI GACGC 1 cut(s) 197
Hin1II CATG 1 cut(s) 341
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 1 cut(s) 194
HpaII CCGG 3 cut(s) 507, 684, 918
HphI GGTGA 2 cut(s) 487, 947
Hpy166II GTNNAC 4 cut(s) 96, 519, 588, 710
Hpy188I TCNGA 4 cut(s) 257, 325, 427, 606
Hpy188III TCNNGA 4 cut(s) 138, 729, 837, 951
Hpy8I GTNNAC 4 cut(s) 96, 519, 588, 710
Hpy99I CGWCG 1 cut(s) 54
HpyAV CCTTC 3 cut(s) 431, 765, 975
HpyCH4IV ACGT 2 cut(s) 52, 762
HpyCH4V TGCA 9 cut(s) 38, 120, 134, 146, 220, 268, 596, 632, 887
HpyF10VI GCNNNNNNNGC 3 cut(s) 666, 914, 920
HpyF3I CTNAG 4 cut(s) 254, 306, 324, 807
HpySE526I ACGT 2 cut(s) 52, 762
Hsp92II CATG 1 cut(s) 341
Kzo9I GATC 2 cut(s) 927, 1003
LmnI GCTCC 4 cut(s) 341, 357, 444, 839
Lsp1109I GCAGC 2 cut(s) 252, 926
LweI GCATC 2 cut(s) 121, 143
MabI ACCWGGT 1 cut(s) 877
MaeI CTAG 3 cut(s) 657, 729, 984
MaeII ACGT 2 cut(s) 52, 762
MaeIII GTNAC 1 cut(s) 988
MalI GATC 2 cut(s) 929, 1005
MboI GATC 2 cut(s) 927, 1003
MboII GAAGA 3 cut(s) 359, 440, 922
MfeI CAATTG 1 cut(s) 365
MhlI GDGCHC 3 cut(s) 449, 844, 910
MluCI AATT 5 cut(s) 319, 328, 365, 379, 577
MmeI TCCRAC 3 cut(s) 408, 629, 783
Mph1103I ATGCAT 1 cut(s) 136
MspI CCGG 3 cut(s) 507, 684, 918
MspR9I CCNGG 4 cut(s) 474, 852, 879, 911
MunI CAATTG 1 cut(s) 365
Mva1269I GAATGC 1 cut(s) 985
MvaI CCWGG 4 cut(s) 474, 852, 879, 911
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 3 cut(s) 666, 914, 920
NdeI CATATG 1 cut(s) 634
NdeII GATC 2 cut(s) 927, 1003
NheI GCTAGC 1 cut(s) 656
NlaIII CATG 1 cut(s) 341
NlaIV GGNNCC 2 cut(s) 907, 908
NsiI ATGCAT 1 cut(s) 136
PaeR7I CTCGAG 1 cut(s) 864
PcsI WCGNNNNNNNCGW 2 cut(s) 46, 862
PctI GAATGC 1 cut(s) 985
PfeI GAWTC 1 cut(s) 194
PflMI CCANNNNNTGG 2 cut(s) 707, 851
PkrI GCNGC 2 cut(s) 267, 916
PpuMI RGGWCCY 2 cut(s) 470, 875
Psp124BI GAGCTC 1 cut(s) 449
Psp5II RGGWCCY 2 cut(s) 470, 875
Psp6I CCWGG 4 cut(s) 472, 850, 877, 909
PspFI CCCAGC 1 cut(s) 902
PspGI CCWGG 4 cut(s) 472, 850, 877, 909
PspN4I GGNNCC 2 cut(s) 907, 908
PspOMI GGGCCC 1 cut(s) 906
PspPI GGNCC 6 cut(s) 470, 638, 710, 875, 906, 907
PspPPI RGGWCCY 2 cut(s) 470, 875
PspXI VCTCGAGB 1 cut(s) 864
PstI CTGCAG 1 cut(s) 270
RsaI GTAC 2 cut(s) 116, 482
RsaNI GTAC 2 cut(s) 115, 481
SacI GAGCTC 1 cut(s) 449
SatI GCNGC 2 cut(s) 266, 915
Sau3AI GATC 2 cut(s) 927, 1003
Sau96I GGNCC 6 cut(s) 470, 638, 710, 875, 906, 907
ScaI AGTACT 1 cut(s) 116
ScrFI CCNGG 4 cut(s) 474, 852, 879, 911
SduI GDGCHC 3 cut(s) 449, 844, 910
SexAI ACCWGGT 1 cut(s) 877
SfaNI GCATC 2 cut(s) 121, 143
SfcI CTRYAG 2 cut(s) 232, 266
Sfr274I CTCGAG 1 cut(s) 864
SinI GGWCC 4 cut(s) 470, 638, 710, 875
SlaI CTCGAG 1 cut(s) 864
SmlI CTYRAG 2 cut(s) 393, 864
SmoI CTYRAG 2 cut(s) 393, 864
Sse9I AATT 5 cut(s) 319, 328, 365, 379, 577
SsiI CCGC 5 cut(s) 191, 660, 674, 698, 743
SspMI CTAG 3 cut(s) 657, 729, 984
SstI GAGCTC 1 cut(s) 449
StyD4I CCNGG 4 cut(s) 472, 850, 877, 909
StyI CCWWGG 3 cut(s) 177, 498, 788
TaiI ACGT 2 cut(s) 55, 765
TaqI TCGA 2 cut(s) 49, 865
TasI AATT 5 cut(s) 319, 328, 365, 379, 577
TatI WGTACW 2 cut(s) 114, 480
TfiI GAWTC 1 cut(s) 194
TseI GCWGC 2 cut(s) 265, 914
TspDTI ATGAA 2 cut(s) 45, 590
TspGWI ACGGA 1 cut(s) 573
Van91I CCANNNNNTGG 2 cut(s) 707, 851
VpaK11BI GGWCC 4 cut(s) 470, 638, 710, 875
XapI RAATTY 4 cut(s) 319, 328, 379, 577
XbaI TCTAGA 1 cut(s) 728
XhoI CTCGAG 1 cut(s) 864
XmiI GTMKAC 2 cut(s) 518, 587
XspI CTAG 3 cut(s) 657, 729, 984
ZrmI AGTACT 1 cut(s) 116
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.