Rw1G033710

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014972
Physical Location & Seq
Reverse (-)
61690931 .. 61692140
1210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G033710.1

Sequence Viewer

Length: 1032 bp
ATGGGGAAACTCTGGGTTGAAGTTTGCTTGATATCTGCTCGAGGGCTCCGGCGTTCGTCGTCCCTATGGAAGCTTCAATGGTATGCTGTTGGGTGGACCAATCCCAATAACAAGTACTGCACCAAGATTGACGCATCTGGAAATGCAAATCCAGACGCGGAATCTAAACTGAATGATTTGGCTCTGCACATTGAAGTCTATAGTAGAGAGCCTATATTCCTCAGAGAAAGGCTACAGGGGACAGCTACTATTCTCTTGAAAGAGTTTCTAGCTAAGCATAACAAGAATTCTGAGAATTCAAGGCATGGAGTTGAAGAAGTTGGGAGCTACCAATTGCGAAAAAAGAATTCCAACAAACCTCAAGGTTTTGTTGATGTTTCAATCCACATTTCTGAGGATATGGAAGGGAGAAGCTCATACACAGGAAATGAGGGAGGACCTGGGGATCACAGCAATACTATCACCTTGGCTACTGGAGATGGATCTGCACCAGCATTACTACCATTTACTCCACAAAAGAGACCAGAACATCAATTCCAAATGAATTCCCCCTATGCAAATCCAATGCCTTCGACTACATACTATTCGAAACCATCTGCTGTTGGACCAAGTTACACACCAGCAAGCGGACCAAGCTACACGCCAGCAAGCGGACCAGGCGACCTGCCAGCAAATGGACCAGGCTACCTGCCAGCAAGCGGACCAAGCTACCTGCCAGCAAGTGGACCAAGCTATCAGCCTTCTAGAACTCCTCCACCGCCTCCACCACCGTCCAATGTTGGATATGTACCTACTTTTCTCCCCAGGACAGATCAAATGACTGACACCTATATCAATATGCCCTCATCATCTGGAGCACCACCTCCTGGTCGTGGAACTCGGGCCGGAGGACCTGCTTTTGCAATGGGAATGGGTGCTGGAGCGCTGGCAGCCGGTGCGGTGATCTTCGGTGATGACTTTATGTCAGGATTTGATGTTCCTTCAGGCTTACAAGATGCTAGTGTTACCATATCAGCTGATCCTCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

343

Amino Acids

36.39

Weight (kDa)

6.6

Isoelectric Point (pI)

59.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40815 AT2G40815
fragaria_vesca FvH4_7g26311
malus_domestica MD01G1172000.v1.1 MD07G1238500.v1.1
prunus_persica Prupe.2G267300_v2.0.a1
pyrus_communis pycom07g21450
rosa_chinensis RchiOBHm_Chr1g0372771
rosa_laevigata RLG00000026873
rosa_multiflora Rmu_sc0029212.1_g000001
rosa_rugosa Rorug01G0042900 Rorug01G0374300
rosa_samantha Rh1AG383000 Rh1CG359900 Rh1DG377600
rosa_wichuraiana Rw1G033710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 672, 696, 720, 901
AccB7I CCANNNNNTGG 3 cut(s) 674, 722, 866
AccII CGCG 1 cut(s) 158
AciI CCGC 6 cut(s) 158, 627, 651, 699, 758, 938
AclWI GGATC 3 cut(s) 453, 490, 1013
AcsI RAATTY 4 cut(s) 286, 295, 346, 544
AcuI CTGAAG 1 cut(s) 966
AfaI GTAC 2 cut(s) 116, 789
AfeI AGCGCT 1 cut(s) 924
AfiI CCNNNNNNNGG 7 cut(s) 626, 650, 674, 698, 722, 866, 872
AgsI TTSAA 7 cut(s) 20, 77, 194, 259, 300, 314, 381
AhdI GACNNNNNGTC 1 cut(s) 961
AjnI CCWGG 5 cut(s) 439, 655, 679, 803, 865
AloI GAACNNNNNNTCC 4 cut(s) 519, 551, 960, 992
AluBI AGCT 9 cut(s) 73, 245, 272, 327, 414, 636, 708, 732, 1016
AluI AGCT 9 cut(s) 73, 245, 272, 327, 414, 636, 708, 732, 1016
Alw21I GWGCWC 1 cut(s) 859
Alw26I GTCTC 1 cut(s) 514
AlwI GGATC 3 cut(s) 453, 490, 1013
Ama87I CYCGRG 2 cut(s) 39, 879
Aor51HI AGCGCT 1 cut(s) 924
AoxI GGCC 1 cut(s) 882
ApeKI GCWGC 1 cut(s) 929
ApoI RAATTY 4 cut(s) 286, 295, 346, 544
AspLEI GCGC 1 cut(s) 925
AsuHPI GGTGA 3 cut(s) 454, 952, 962
AsuII TTCGAA 1 cut(s) 587
AvaI CYCGRG 2 cut(s) 39, 879
AvaII GGWCC 9 cut(s) 96, 437, 605, 629, 653, 677, 701, 725, 890
BanII GRGCYC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 859
BbvI GCAGC 1 cut(s) 941
BccI CCATC 2 cut(s) 473, 601
BciT130I CCWGG 5 cut(s) 441, 657, 681, 805, 867
BcoDI GTCTC 1 cut(s) 514
BfaI CTAG 3 cut(s) 269, 744, 999
BfmI CTRYAG 2 cut(s) 199, 233
BfoI RGCGCY 1 cut(s) 926
BfuAI ACCTGC 4 cut(s) 672, 696, 720, 901
BisI GCNGC 1 cut(s) 930
BlpI GCTNAGC 1 cut(s) 273
BlsI GCNGC 1 cut(s) 931
BmcAI AGTACT 1 cut(s) 116
Bme1390I CCNGG 5 cut(s) 441, 657, 681, 805, 867
Bme18I GGWCC 9 cut(s) 96, 437, 605, 629, 653, 677, 701, 725, 890
BmeRI GACNNNNNGTC 1 cut(s) 961
BmeT110I CYCGRG 2 cut(s) 39, 879
BmiI GGNNCC 1 cut(s) 47
BmrFI CCNGG 5 cut(s) 441, 657, 681, 805, 867
BmsI GCATC 2 cut(s) 143, 985
BpmI CTGGAG 3 cut(s) 495, 873, 939
Bpu1102I GCTNAGC 1 cut(s) 273
Bpu14I TTCGAA 1 cut(s) 587
BpuEI CTTGAG 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 514
BsaJI CCNNGG 3 cut(s) 440, 465, 803
Bsc4I CCNNNNNNNGG 7 cut(s) 626, 650, 674, 698, 722, 866, 872
Bse118I RCCGGY 1 cut(s) 932
Bse1I ACTGG 1 cut(s) 478
Bse3DI GCAATG 1 cut(s) 909
BseBI CCWGG 5 cut(s) 441, 657, 681, 805, 867
BseDI CCNNGG 3 cut(s) 440, 465, 803
BseLI CCNNNNNNNGG 7 cut(s) 626, 650, 674, 698, 722, 866, 872
BseMI GCAATG 1 cut(s) 909
BseMII CTCAG 3 cut(s) 235, 282, 384
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 1 cut(s) 741
BseXI GCAGC 1 cut(s) 941
BsgI GTGCAG 3 cut(s) 103, 170, 471
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 1 cut(s) 884
BsiHKAI GWGCWC 1 cut(s) 859
BsiHKCI CYCGRG 2 cut(s) 39, 879
BsiSI CCGG 3 cut(s) 49, 885, 933
BslFI GGGAC 2 cut(s) 46, 253
BslI CCNNNNNNNGG 7 cut(s) 626, 650, 674, 698, 722, 866, 872
BsmAI GTCTC 1 cut(s) 514
BsmFI GGGAC 2 cut(s) 46, 253
BsnI GGCC 1 cut(s) 884
Bso31I GGTCTC 1 cut(s) 514
BsoBI CYCGRG 2 cut(s) 39, 879
Bsp119I TTCGAA 1 cut(s) 587
Bsp1286I GDGCHC 2 cut(s) 48, 859
Bsp143I GATC 5 cut(s) 445, 482, 811, 942, 1018
Bsp1720I GCTNAGC 1 cut(s) 273
BspACI CCGC 6 cut(s) 158, 627, 651, 699, 758, 938
BspANI GGCC 1 cut(s) 884
BspCNI CTCAG 3 cut(s) 234, 283, 385
BspFNI CGCG 1 cut(s) 158
BspLI GGNNCC 1 cut(s) 47
BspMI ACCTGC 4 cut(s) 672, 696, 720, 901
BspPI GGATC 3 cut(s) 453, 490, 1013
BspT104I TTCGAA 1 cut(s) 587
BspTNI GGTCTC 1 cut(s) 514
BsrDI GCAATG 1 cut(s) 909
BsrFI RCCGGY 1 cut(s) 932
BsrI ACTGG 1 cut(s) 478
BssAI RCCGGY 1 cut(s) 932
BssECI CCNNGG 3 cut(s) 440, 465, 803
BssMI GATC 5 cut(s) 445, 482, 811, 942, 1018
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 5 cut(s) 441, 657, 681, 805, 867
Bst4CI ACNGT 1 cut(s) 771
BstAPI GCANNNNNTGC 1 cut(s) 935
BstBI TTCGAA 1 cut(s) 587
BstC8I GCNNGC 8 cut(s) 625, 645, 649, 669, 693, 697, 717, 927
BstDEI CTNAG 4 cut(s) 221, 273, 291, 393
BstFNI CGCG 1 cut(s) 158
BstH2I RGCGCY 1 cut(s) 926
BstHHI GCGC 1 cut(s) 925
BstKTI GATC 5 cut(s) 448, 485, 814, 945, 1021
BstMAI GTCTC 1 cut(s) 514
BstMBI GATC 5 cut(s) 445, 482, 811, 942, 1018
BstMWI GCNNNNNNNGC 5 cut(s) 633, 657, 705, 929, 935
BstNI CCWGG 5 cut(s) 441, 657, 681, 805, 867
BstSCI CCNGG 5 cut(s) 439, 655, 679, 803, 865
BstSFI CTRYAG 2 cut(s) 199, 233
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 941
BstX2I RGATCY 1 cut(s) 482
BstYI RGATCY 1 cut(s) 482
BsuRI GGCC 1 cut(s) 884
BveI ACCTGC 4 cut(s) 672, 696, 720, 901
Cac8I GCNNGC 8 cut(s) 625, 645, 649, 669, 693, 697, 717, 927
CfoI GCGC 1 cut(s) 925
Cfr10I RCCGGY 1 cut(s) 932
CseI GACGC 2 cut(s) 140, 164
Csp6I GTAC 2 cut(s) 115, 788
CviAII CATG 1 cut(s) 305
CviQI GTAC 2 cut(s) 115, 788
DdeI CTNAG 4 cut(s) 221, 273, 291, 393
DpnI GATC 5 cut(s) 447, 484, 813, 944, 1020
DpnII GATC 5 cut(s) 445, 482, 811, 942, 1018
DriI GACNNNNNGTC 1 cut(s) 961
Eam1105I GACNNNNNGTC 1 cut(s) 961
Eco130I CCWWGG 1 cut(s) 465
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 1 cut(s) 514
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 9 cut(s) 96, 437, 605, 629, 653, 677, 701, 725, 890
Eco47III AGCGCT 1 cut(s) 924
Eco57I CTGAAG 1 cut(s) 966
Eco88I CYCGRG 2 cut(s) 39, 879
EcoO109I RGGNCCY 2 cut(s) 437, 890
EcoRI GAATTC 4 cut(s) 286, 295, 346, 544
EcoRII CCWGG 5 cut(s) 439, 655, 679, 803, 865
EcoRV GATATC 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 465
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 1 cut(s) 308
FaqI GGGAC 2 cut(s) 46, 253
FatI CATG 1 cut(s) 304
Fnu4HI GCNGC 1 cut(s) 930
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 1 cut(s) 930
FspBI CTAG 3 cut(s) 269, 744, 999
GlaI GCGC 1 cut(s) 924
GluI GCNGC 1 cut(s) 930
GsuI CTGGAG 3 cut(s) 495, 873, 939
HaeII RGCGCY 1 cut(s) 926
HaeIII GGCC 1 cut(s) 884
HapII CCGG 3 cut(s) 49, 885, 933
HgaI GACGC 2 cut(s) 140, 164
HhaI GCGC 1 cut(s) 925
Hin1II CATG 1 cut(s) 308
Hin6I GCGC 1 cut(s) 923
HinP1I GCGC 1 cut(s) 923
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 1 cut(s) 161
HpaII CCGG 3 cut(s) 49, 885, 933
HphI GGTGA 3 cut(s) 454, 952, 962
Hpy166II GTNNAC 2 cut(s) 96, 725
Hpy188I TCNGA 3 cut(s) 224, 292, 394
Hpy188III TCNNGA 6 cut(s) 138, 152, 256, 744, 852, 966
Hpy8I GTNNAC 2 cut(s) 96, 725
Hpy99I CGWCG 1 cut(s) 61
HpyAV CCTTC 4 cut(s) 398, 579, 750, 990
HpyCH4III ACNGT 1 cut(s) 771
HpyCH4V TGCA 6 cut(s) 120, 146, 187, 488, 557, 902
HpyF10VI GCNNNNNNNGC 5 cut(s) 633, 657, 705, 929, 935
HpyF3I CTNAG 4 cut(s) 221, 273, 291, 393
Hsp92II CATG 1 cut(s) 308
HspAI GCGC 1 cut(s) 923
Kzo9I GATC 5 cut(s) 445, 482, 811, 942, 1018
LmnI GCTCC 4 cut(s) 51, 324, 854, 920
Lsp1109I GCAGC 1 cut(s) 941
LweI GCATC 2 cut(s) 143, 985
MaeI CTAG 3 cut(s) 269, 744, 999
MaeIII GTNAC 2 cut(s) 611, 1003
MalI GATC 5 cut(s) 447, 484, 813, 944, 1020
MboI GATC 5 cut(s) 445, 482, 811, 942, 1018
MboII GAAGA 2 cut(s) 326, 937
MfeI CAATTG 1 cut(s) 332
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 2 cut(s) 48, 859
MluCI AATT 6 cut(s) 286, 295, 332, 346, 533, 544
MmeI TCCRAC 3 cut(s) 375, 583, 760
MspA1I CMGCKG 1 cut(s) 1016
MspI CCGG 3 cut(s) 49, 885, 933
MspR9I CCNGG 5 cut(s) 441, 657, 681, 805, 867
MunI CAATTG 1 cut(s) 332
MvaI CCWGG 5 cut(s) 441, 657, 681, 805, 867
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 5 cut(s) 633, 657, 705, 929, 935
NdeII GATC 5 cut(s) 445, 482, 811, 942, 1018
NlaIII CATG 1 cut(s) 308
NlaIV GGNNCC 1 cut(s) 47
NspV TTCGAA 1 cut(s) 587
PaeR7I CTCGAG 1 cut(s) 39
PfeI GAWTC 1 cut(s) 161
PflMI CCANNNNNTGG 3 cut(s) 674, 722, 866
PkrI GCNGC 1 cut(s) 931
PpuMI RGGWCCY 2 cut(s) 437, 890
Psp5II RGGWCCY 2 cut(s) 437, 890
Psp6I CCWGG 5 cut(s) 439, 655, 679, 803, 865
PspGI CCWGG 5 cut(s) 439, 655, 679, 803, 865
PspN4I GGNNCC 1 cut(s) 47
PspPPI RGGWCCY 2 cut(s) 437, 890
PspXI VCTCGAGB 1 cut(s) 39
PsuI RGATCY 1 cut(s) 482
PvuII CAGCTG 1 cut(s) 1016
RsaI GTAC 2 cut(s) 116, 789
RsaNI GTAC 2 cut(s) 115, 788
SatI GCNGC 1 cut(s) 930
Sau3AI GATC 5 cut(s) 445, 482, 811, 942, 1018
ScaI AGTACT 1 cut(s) 116
ScrFI CCNGG 5 cut(s) 441, 657, 681, 805, 867
SduI GDGCHC 2 cut(s) 48, 859
SfaNI GCATC 2 cut(s) 143, 985
SfcI CTRYAG 2 cut(s) 199, 233
Sfr274I CTCGAG 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 587
SinI GGWCC 9 cut(s) 96, 437, 605, 629, 653, 677, 701, 725, 890
SlaI CTCGAG 1 cut(s) 39
SmlI CTYRAG 2 cut(s) 39, 360
SmoI CTYRAG 2 cut(s) 39, 360
Sse9I AATT 6 cut(s) 286, 295, 332, 346, 533, 544
SsiI CCGC 6 cut(s) 158, 627, 651, 699, 758, 938
SspMI CTAG 3 cut(s) 269, 744, 999
StyD4I CCNGG 5 cut(s) 439, 655, 679, 803, 865
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 1 cut(s) 771
TaqI TCGA 3 cut(s) 40, 572, 587
TasI AATT 6 cut(s) 286, 295, 332, 346, 533, 544
TatI WGTACW 1 cut(s) 114
TfiI GAWTC 1 cut(s) 161
TseI GCWGC 1 cut(s) 929
TspDTI ATGAA 1 cut(s) 557
Van91I CCANNNNNTGG 3 cut(s) 674, 722, 866
VpaK11BI GGWCC 9 cut(s) 96, 437, 605, 629, 653, 677, 701, 725, 890
XapI RAATTY 4 cut(s) 286, 295, 346, 544
XbaI TCTAGA 1 cut(s) 743
XhoI CTCGAG 1 cut(s) 39
XspI CTAG 3 cut(s) 269, 744, 999
ZrmI AGTACT 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.