Rh1AG383000

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0014972
Physical Location & Seq
Reverse (-)
61400263 .. 61403314
3052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG383000.1

Sequence Viewer

Length: 1947 bp
ATGGTGATTATTCCCCCACACATTATCATCCCCACCTCATTGGAGAAACAGCCTTCAGAGGAAGAAATGGAGGCTGCGACGCCCATGAACATAGTAGAGGGTCAGTCATGCAAGGCTATGGAGTTAAAATGGGTAAGTTCCCGAGGCAAAACCCAACTGCAAAACATGAAAACACCTTCAAAATCAAAAGCACCAGCTACACTTCAAGCGTCAGTCCCTGTGTTCATATCCACAAACCCATCTCACGTAAAACCAGATGATCTCGTTGAGCTATACAGCGCCTGCAATCTCAACGGCCACCGATTCCCGGAGTACGTGGACGGCGGAGATGGCTGCCGTGTAGTTGAAGGTATTGATGTGAACAAGCTGCGTGTGGCTCTCTCACACAGTGAAGTCGTGGTCTCCGTCTTCTGCAAAGCAAATGATGTAGTTAGCAAAACTTCATCATCATTTTCGCAAAAAAATCGACAGCAGCAGAAGATTACGGAGGGTTTAGGAGAATTGTTTGAGAGGGTGGTGATGCCGGTGACACCATTTAACAGTCAGTTGGTGGGTTTTGGCCGAGCTGTTTCTGATCAAGGATTGACAGCCTCCATCTACGATGTTATGGTTATTCCTTCATTGCAGGGTATGGGAATTGGCAAGATGATAGTTAAAAGAATCACAAGAATACTCACAAGTAGAGACATTTATGACATAGCAGCCCTTTGCTCAGAGAGTGAGAGCTCATTCTTCGAAGCCTGTGGTTTTGGAGATGACATTCTGTGCTCCACAGCAATGATGTATACAAGGACTGCTGGTGGTTCAACTAATAATCTCGAAGAACAACGGATGGTAACACGCGCAGGCATAAGAATTATCTCACCAGCAAATTATCCTGGTATGGGGAAACTCTGGGTTGAAGTTTGCTTGATATCTGCTCGAGGGCTCCGGCGTTCGTCGTCCCTATGGAAGCTTCAATGGTATGCTGTTGGGTGGACCAATCCCAATAACAAGTACTGCACCAAGATTGATGCATCTGGAAATGCAAATCCAGTATGGAAAACCAAGTTTGCTACCTTGGTTGAGGACGCGGAATCTAAACTGAATGATTTGGCTCTGCACATTGAAGTCTATAGTAGAGAGCCTATATTCCTCAGAGAAAGGCTACAGGGGACAGCTACTATTCTCTTGAAAGAGTTTCTGGCCAAGCATAACAAGAATTCTGAGAATTCAAGGCATGGAGTTGAAGAAGTTGGGAGCTACCAATTGCGAAAAAAGAATTCCAACAAACCTCAAGGTTTTGTTGATGTTTCAATCCACATTTCTGAGGATATGGAAGGGAGAAGCTCATACACAGGAAATGAGGGAGGACCTGGGGATCACAGCAATACTATCACCTTGGCTACCGGAGATGGATCTGCACCAGCATTACTACCATTTACTCCACAAAAGAGACCAGAACATCAATTCCAAATGAATTCCCCCTATGCAAATCCAATGCCTTCGACTACATACTATTCGAAACCATCTGCTGTTGGACCAAGTTACACACCAGCAAGCGGACCAAGCTACACGCCAGCAAGCGGACCAGGCAACCTGCCAGCAAATGGACCAGGCTACCTGCCAGCAAGCGGACCAAGCTACCTGCCAGCAAGTGGACCAAGCTATCAGCCTTCTAGAACTCCTCCACCGCCTCCACCACCTTCCAATGTTGGATATGTACCTACTTTTCTCCCCAGGACAGATCAAATGACTGACACCTATATCAATATGCCCTCATCATCTGGAGCACCACCTCCTGGTCGTGGAACTCGGGCCGGAGGACCTGCTTTTGCAATGGGAATGGGTGCTGGAGCGCTGGCAGCCGGTGCGGTGATCTTCGGTGATGACTTTATGTCAGGATTTGATGTTCCTTCAGGCTTACAAGATGCTAGTGTTACCATATCAGCTGATCCTCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

648

Amino Acids

69.69

Weight (kDa)

6.81

Isoelectric Point (pI)

55.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 177 - 250 6.9e-07 Acetyltransferase (GNAT) family
C2 PF00168 300 - 399 6.4e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40815 AT2G40815
fragaria_vesca FvH4_7g26311
malus_domestica MD01G1172000.v1.1 MD07G1238500.v1.1
prunus_persica Prupe.2G267300_v2.0.a1
pyrus_communis pycom07g21450
rosa_chinensis RchiOBHm_Chr1g0372771
rosa_laevigata RLG00000026873
rosa_multiflora Rmu_sc0029212.1_g000001
rosa_rugosa Rorug01G0042900 Rorug01G0374300
rosa_samantha Rh1AG383000 Rh1CG359900 Rh1DG377600
rosa_wichuraiana Rw1G033710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 1587, 1611, 1635, 1816
AccB7I CCANNNNNTGG 3 cut(s) 1589, 1637, 1781
AccI GTMKAC 1 cut(s) 785
AccII CGCG 2 cut(s) 843, 1073
AciI CCGC 7 cut(s) 324, 1073, 1542, 1566, 1614, 1673, 1853
AclWI GGATC 3 cut(s) 1368, 1405, 1928
AcoI YGGCCR 3 cut(s) 295, 559, 1185
AcsI RAATTY 4 cut(s) 1201, 1210, 1261, 1459
AcuI CTGAAG 2 cut(s) 39, 1881
AcyI GRCGYC 1 cut(s) 80
AdeI CACNNNGTG 1 cut(s) 389
AfaI GTAC 3 cut(s) 314, 998, 1704
AfeI AGCGCT 1 cut(s) 1839
AfiI CCNNNNNNNGG 9 cut(s) 307, 884, 1541, 1565, 1589, 1613, 1637, 1781, 1787
AhdI GACNNNNNGTC 1 cut(s) 1876
AjnI CCWGG 6 cut(s) 877, 1354, 1570, 1594, 1718, 1780
AloI GAACNNNNNNTCC 4 cut(s) 1434, 1466, 1875, 1907
Alw21I GWGCWC 3 cut(s) 728, 770, 1774
Alw26I GTCTC 3 cut(s) 406, 678, 1429
AlwI GGATC 3 cut(s) 1368, 1405, 1928
AlwNI CAGNNNCTG 2 cut(s) 218, 282
Ama87I CYCGRG 3 cut(s) 141, 921, 1794
Aor51HI AGCGCT 1 cut(s) 1839
AoxI GGCC 4 cut(s) 295, 559, 1185, 1797
ApeKI GCWGC 6 cut(s) 74, 333, 367, 472, 701, 1844
ApoI RAATTY 4 cut(s) 1201, 1210, 1261, 1459
AspLEI GCGC 3 cut(s) 281, 845, 1840
AsuC2I CCSGG 1 cut(s) 308
AsuHPI GGTGA 7 cut(s) 16, 529, 538, 855, 1369, 1867, 1877
AsuII TTCGAA 2 cut(s) 735, 1502
AvaI CYCGRG 3 cut(s) 141, 921, 1794
AvaII GGWCC 9 cut(s) 978, 1352, 1520, 1544, 1568, 1592, 1616, 1640, 1805
BalI TGGCCA 1 cut(s) 1187
BanII GRGCYC 2 cut(s) 728, 930
BbsI GAAGAC 1 cut(s) 400
Bbv12I GWGCWC 3 cut(s) 728, 770, 1774
BbvI GCAGC 6 cut(s) 61, 320, 354, 484, 713, 1856
BccI CCATC 6 cut(s) 247, 323, 602, 826, 1388, 1516
BceAI ACGGC 3 cut(s) 310, 321, 337
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BciT130I CCWGG 6 cut(s) 879, 1356, 1572, 1596, 1720, 1782
BclI TGATCA 1 cut(s) 574
BcnI CCSGG 1 cut(s) 308
BcoDI GTCTC 3 cut(s) 406, 678, 1429
BfaI CTAG 2 cut(s) 1659, 1914
BfmI CTRYAG 2 cut(s) 1114, 1148
BfoI RGCGCY 2 cut(s) 282, 1841
BfuAI ACCTGC 4 cut(s) 1587, 1611, 1635, 1816
BisI GCNGC 6 cut(s) 75, 334, 368, 473, 702, 1845
BlsI GCNGC 6 cut(s) 76, 335, 369, 474, 703, 1846
BmcAI AGTACT 1 cut(s) 998
Bme1390I CCNGG 7 cut(s) 308, 879, 1356, 1572, 1596, 1720, 1782
Bme18I GGWCC 9 cut(s) 978, 1352, 1520, 1544, 1568, 1592, 1616, 1640, 1805
BmeRI GACNNNNNGTC 1 cut(s) 1876
BmeT110I CYCGRG 3 cut(s) 141, 921, 1794
BmiI GGNNCC 1 cut(s) 929
BmrFI CCNGG 7 cut(s) 308, 879, 1356, 1572, 1596, 1720, 1782
BmsI GCATC 4 cut(s) 510, 1003, 1025, 1900
BpiI GAAGAC 1 cut(s) 400
BpmI CTGGAG 2 cut(s) 1788, 1854
Bpu14I TTCGAA 2 cut(s) 735, 1502
BpuEI CTTGAG 1 cut(s) 1260
BpuMI CCSGG 1 cut(s) 308
BsaAI YACGTR 2 cut(s) 247, 316
BsaHI GRCGYC 1 cut(s) 80
BsaI GGTCTC 2 cut(s) 406, 1429
BsaJI CCNNGG 5 cut(s) 142, 1059, 1355, 1380, 1718
BsaWI WCCGGW 1 cut(s) 1388
Bsc4I CCNNNNNNNGG 9 cut(s) 307, 884, 1541, 1565, 1589, 1613, 1637, 1781, 1787
Bse118I RCCGGY 2 cut(s) 523, 1847
Bse1I ACTGG 1 cut(s) 1034
Bse3DI GCAATG 3 cut(s) 620, 783, 1824
BseBI CCWGG 6 cut(s) 879, 1356, 1572, 1596, 1720, 1782
BseDI CCNNGG 5 cut(s) 142, 1059, 1355, 1380, 1718
BseGI GGATG 2 cut(s) 27, 837
BseLI CCNNNNNNNGG 9 cut(s) 307, 884, 1541, 1565, 1589, 1613, 1637, 1781, 1787
BseMI GCAATG 3 cut(s) 620, 783, 1824
BseMII CTCAG 4 cut(s) 726, 1150, 1197, 1299
BseNI ACTGG 1 cut(s) 1034
BseRI GAGGAG 1 cut(s) 1656
BseXI GCAGC 6 cut(s) 61, 320, 354, 484, 713, 1856
BsgI GTGCAG 3 cut(s) 985, 1085, 1386
Bsh1236I CGCG 2 cut(s) 843, 1073
BshFI GGCC 4 cut(s) 297, 561, 1187, 1799
BsiHKAI GWGCWC 3 cut(s) 728, 770, 1774
BsiHKCI CYCGRG 3 cut(s) 141, 921, 1794
BsiSI CCGG 6 cut(s) 308, 524, 931, 1389, 1800, 1848
BslFI GGGAC 3 cut(s) 200, 928, 1168
BslI CCNNNNNNNGG 9 cut(s) 307, 884, 1541, 1565, 1589, 1613, 1637, 1781, 1787
BsmAI GTCTC 3 cut(s) 406, 678, 1429
BsmFI GGGAC 3 cut(s) 200, 928, 1168
BsnI GGCC 4 cut(s) 297, 561, 1187, 1799
Bso31I GGTCTC 2 cut(s) 406, 1429
BsoBI CYCGRG 3 cut(s) 141, 921, 1794
Bsp119I TTCGAA 2 cut(s) 735, 1502
Bsp1286I GDGCHC 4 cut(s) 728, 770, 930, 1774
Bsp143I GATC 7 cut(s) 259, 574, 1360, 1397, 1726, 1857, 1933
BspACI CCGC 7 cut(s) 324, 1073, 1542, 1566, 1614, 1673, 1853
BspANI GGCC 4 cut(s) 297, 561, 1187, 1799
BspCNI CTCAG 4 cut(s) 725, 1149, 1198, 1300
BspFNI CGCG 2 cut(s) 843, 1073
BspLI GGNNCC 1 cut(s) 929
BspMI ACCTGC 4 cut(s) 1587, 1611, 1635, 1816
BspPI GGATC 3 cut(s) 1368, 1405, 1928
BspT104I TTCGAA 2 cut(s) 735, 1502
BspTNI GGTCTC 2 cut(s) 406, 1429
BsrDI GCAATG 3 cut(s) 620, 783, 1824
BsrFI RCCGGY 2 cut(s) 523, 1847
BsrI ACTGG 1 cut(s) 1034
BssAI RCCGGY 2 cut(s) 523, 1847
BssECI CCNNGG 5 cut(s) 142, 1059, 1355, 1380, 1718
BssMI GATC 7 cut(s) 259, 574, 1360, 1397, 1726, 1857, 1933
BssNAI GTATAC 1 cut(s) 786
BssNI GRCGYC 1 cut(s) 80
BssT1I CCWWGG 2 cut(s) 1059, 1380
Bst1107I GTATAC 1 cut(s) 786
Bst2UI CCWGG 6 cut(s) 879, 1356, 1572, 1596, 1720, 1782
Bst4CI ACNGT 2 cut(s) 389, 542
BstACI GRCGYC 1 cut(s) 80
BstAPI GCANNNNNTGC 1 cut(s) 1850
BstBAI YACGTR 2 cut(s) 247, 316
BstBI TTCGAA 2 cut(s) 735, 1502
BstDEI CTNAG 4 cut(s) 712, 1136, 1206, 1308
BstF5I GGATG 2 cut(s) 27, 837
BstFNI CGCG 2 cut(s) 843, 1073
BstH2I RGCGCY 2 cut(s) 282, 1841
BstHHI GCGC 3 cut(s) 281, 845, 1840
BstKTI GATC 7 cut(s) 262, 577, 1363, 1400, 1729, 1860, 1936
BstMAI GTCTC 3 cut(s) 406, 678, 1429
BstMBI GATC 7 cut(s) 259, 574, 1360, 1397, 1726, 1857, 1933
BstMWI GCNNNNNNNGC 6 cut(s) 330, 1548, 1572, 1620, 1844, 1850
BstNI CCWGG 6 cut(s) 879, 1356, 1572, 1596, 1720, 1782
BstSCI CCNGG 7 cut(s) 306, 877, 1354, 1570, 1594, 1718, 1780
BstSFI CTRYAG 2 cut(s) 1114, 1148
BstUI CGCG 2 cut(s) 843, 1073
BstV1I GCAGC 6 cut(s) 61, 320, 354, 484, 713, 1856
BstV2I GAAGAC 1 cut(s) 400
BstX2I RGATCY 1 cut(s) 1397
BstXI CCANNNNNNTGG 1 cut(s) 40
BstYI RGATCY 1 cut(s) 1397
BstZ17I GTATAC 1 cut(s) 786
BsuRI GGCC 4 cut(s) 297, 561, 1187, 1799
BtsCI GGATG 2 cut(s) 27, 837
BtsIMutI CAGTG 1 cut(s) 394
BveI ACCTGC 4 cut(s) 1587, 1611, 1635, 1816
CaiI CAGNNNCTG 2 cut(s) 218, 282
CfoI GCGC 3 cut(s) 281, 845, 1840
Cfr10I RCCGGY 2 cut(s) 523, 1847
CseI GACGC 3 cut(s) 88, 198, 1079
Csp6I GTAC 3 cut(s) 313, 997, 1703
CviAII CATG 4 cut(s) 85, 108, 166, 1220
CviQI GTAC 3 cut(s) 313, 997, 1703
DdeI CTNAG 4 cut(s) 712, 1136, 1206, 1308
DpnI GATC 7 cut(s) 261, 576, 1362, 1399, 1728, 1859, 1935
DpnII GATC 7 cut(s) 259, 574, 1360, 1397, 1726, 1857, 1933
DraIII CACNNNGTG 1 cut(s) 389
DriI GACNNNNNGTC 1 cut(s) 1876
EaeI YGGCCR 3 cut(s) 295, 559, 1185
Eam1105I GACNNNNNGTC 1 cut(s) 1876
EciI GGCGGA 1 cut(s) 339
Ecl136II GAGCTC 1 cut(s) 726
Eco130I CCWWGG 2 cut(s) 1059, 1380
Eco24I GRGCYC 2 cut(s) 728, 930
Eco31I GGTCTC 2 cut(s) 406, 1429
Eco32I GATATC 1 cut(s) 915
Eco47I GGWCC 9 cut(s) 978, 1352, 1520, 1544, 1568, 1592, 1616, 1640, 1805
Eco47III AGCGCT 1 cut(s) 1839
Eco53kI GAGCTC 1 cut(s) 726
Eco57I CTGAAG 2 cut(s) 39, 1881
Eco88I CYCGRG 3 cut(s) 141, 921, 1794
EcoICRI GAGCTC 1 cut(s) 726
EcoO109I RGGNCCY 2 cut(s) 1352, 1805
EcoRI GAATTC 4 cut(s) 1201, 1210, 1261, 1459
EcoRII CCWGG 6 cut(s) 877, 1354, 1570, 1594, 1718, 1780
EcoRV GATATC 1 cut(s) 915
EcoT14I CCWWGG 2 cut(s) 1059, 1380
EcoT22I ATGCAT 1 cut(s) 1018
EcoT38I GRGCYC 2 cut(s) 728, 930
ErhI CCWWGG 2 cut(s) 1059, 1380
FaeI CATG 4 cut(s) 88, 111, 169, 1223
FaqI GGGAC 3 cut(s) 200, 928, 1168
FatI CATG 4 cut(s) 84, 107, 165, 1219
FbaI TGATCA 1 cut(s) 574
FblI GTMKAC 1 cut(s) 785
Fnu4HI GCNGC 6 cut(s) 75, 334, 368, 473, 702, 1845
FokI GGATG 2 cut(s) 14, 844
FriOI GRGCYC 2 cut(s) 728, 930
Fsp4HI GCNGC 6 cut(s) 75, 334, 368, 473, 702, 1845
FspBI CTAG 2 cut(s) 1659, 1914
GlaI GCGC 3 cut(s) 280, 844, 1839
GluI GCNGC 6 cut(s) 75, 334, 368, 473, 702, 1845
GsuI CTGGAG 2 cut(s) 1788, 1854
HaeII RGCGCY 2 cut(s) 282, 1841
HaeIII GGCC 4 cut(s) 297, 561, 1187, 1799
HapII CCGG 6 cut(s) 308, 524, 931, 1389, 1800, 1848
HgaI GACGC 3 cut(s) 88, 198, 1079
HhaI GCGC 3 cut(s) 281, 845, 1840
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 4 cut(s) 88, 111, 169, 1223
Hin6I GCGC 3 cut(s) 279, 843, 1838
HinP1I GCGC 3 cut(s) 279, 843, 1838
HindIII AAGCTT 1 cut(s) 953
HinfI GANTC 3 cut(s) 303, 660, 1076
HpaII CCGG 6 cut(s) 308, 524, 931, 1389, 1800, 1848
HphI GGTGA 7 cut(s) 16, 529, 538, 855, 1369, 1867, 1877
Hpy166II GTNNAC 5 cut(s) 319, 361, 786, 978, 1640
Hpy188I TCNGA 6 cut(s) 58, 574, 715, 1139, 1207, 1309
Hpy188III TCNNGA 7 cut(s) 141, 818, 1020, 1171, 1659, 1767, 1881
Hpy8I GTNNAC 5 cut(s) 319, 361, 786, 978, 1640
Hpy99I CGWCG 2 cut(s) 82, 943
HpyAV CCTTC 9 cut(s) 63, 186, 341, 627, 1313, 1494, 1665, 1695, 1905
HpyCH4III ACNGT 2 cut(s) 389, 542
HpyCH4IV ACGT 2 cut(s) 246, 315
HpyF10VI GCNNNNNNNGC 6 cut(s) 330, 1548, 1572, 1620, 1844, 1850
HpyF3I CTNAG 4 cut(s) 712, 1136, 1206, 1308
HpySE526I ACGT 2 cut(s) 246, 315
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 4 cut(s) 88, 111, 169, 1223
HspAI GCGC 3 cut(s) 279, 843, 1838
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 7 cut(s) 259, 574, 1360, 1397, 1726, 1857, 1933
LmnI GCTCC 5 cut(s) 773, 933, 1239, 1769, 1835
Lsp1109I GCAGC 6 cut(s) 61, 320, 354, 484, 713, 1856
LweI GCATC 4 cut(s) 510, 1003, 1025, 1900
MaeI CTAG 2 cut(s) 1659, 1914
MaeII ACGT 2 cut(s) 246, 315
MaeIII GTNAC 4 cut(s) 526, 835, 1526, 1918
MalI GATC 7 cut(s) 261, 576, 1362, 1399, 1728, 1859, 1935
MboI GATC 7 cut(s) 259, 574, 1360, 1397, 1726, 1857, 1933
MboII GAAGA 7 cut(s) 74, 400, 490, 724, 833, 1241, 1852
MfeI CAATTG 1 cut(s) 1247
MflI RGATCY 1 cut(s) 1397
MhlI GDGCHC 4 cut(s) 728, 770, 930, 1774
MlsI TGGCCA 1 cut(s) 1187
MluNI TGGCCA 1 cut(s) 1187
MmeI TCCRAC 3 cut(s) 1290, 1498, 1675
Mox20I TGGCCA 1 cut(s) 1187
Mph1103I ATGCAT 1 cut(s) 1018
MscI TGGCCA 1 cut(s) 1187
MseI TTAA 3 cut(s) 125, 537, 654
MslI CAYNNNNRTG 1 cut(s) 776
Msp20I TGGCCA 1 cut(s) 1187
MspA1I CMGCKG 1 cut(s) 1931
MspI CCGG 6 cut(s) 308, 524, 931, 1389, 1800, 1848
MspR9I CCNGG 7 cut(s) 308, 879, 1356, 1572, 1596, 1720, 1782
MunI CAATTG 1 cut(s) 1247
MvaI CCWGG 6 cut(s) 879, 1356, 1572, 1596, 1720, 1782
MvnI CGCG 2 cut(s) 843, 1073
MwoI GCNNNNNNNGC 6 cut(s) 330, 1548, 1572, 1620, 1844, 1850
NciI CCSGG 1 cut(s) 308
NdeII GATC 7 cut(s) 259, 574, 1360, 1397, 1726, 1857, 1933
NlaIII CATG 4 cut(s) 88, 111, 169, 1223
NlaIV GGNNCC 1 cut(s) 929
NmeAIII GCCGAG 1 cut(s) 587
NmuCI GTSAC 1 cut(s) 526
NsiI ATGCAT 1 cut(s) 1018
NspV TTCGAA 2 cut(s) 735, 1502
PaeR7I CTCGAG 1 cut(s) 921
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PfeI GAWTC 3 cut(s) 303, 660, 1076
PflMI CCANNNNNTGG 3 cut(s) 1589, 1637, 1781
PfoI TCCNGGA 1 cut(s) 306
PkrI GCNGC 6 cut(s) 76, 335, 369, 474, 703, 1846
Ppu21I YACGTR 2 cut(s) 247, 316
PpuMI RGGWCCY 2 cut(s) 1352, 1805
Psp124BI GAGCTC 1 cut(s) 728
Psp5II RGGWCCY 2 cut(s) 1352, 1805
Psp6I CCWGG 6 cut(s) 877, 1354, 1570, 1594, 1718, 1780
PspGI CCWGG 6 cut(s) 877, 1354, 1570, 1594, 1718, 1780
PspN4I GGNNCC 1 cut(s) 929
PspPPI RGGWCCY 2 cut(s) 1352, 1805
PspXI VCTCGAGB 1 cut(s) 921
PstNI CAGNNNCTG 2 cut(s) 218, 282
PsuI RGATCY 1 cut(s) 1397
PvuII CAGCTG 1 cut(s) 1931
RsaI GTAC 3 cut(s) 314, 998, 1704
RsaNI GTAC 3 cut(s) 313, 997, 1703
RseI CAYNNNNRTG 1 cut(s) 776
SacI GAGCTC 1 cut(s) 728
SaqAI TTAA 3 cut(s) 125, 537, 654
SatI GCNGC 6 cut(s) 75, 334, 368, 473, 702, 1845
Sau3AI GATC 7 cut(s) 259, 574, 1360, 1397, 1726, 1857, 1933
ScaI AGTACT 1 cut(s) 998
ScrFI CCNGG 7 cut(s) 308, 879, 1356, 1572, 1596, 1720, 1782
SduI GDGCHC 4 cut(s) 728, 770, 930, 1774
SfaNI GCATC 4 cut(s) 510, 1003, 1025, 1900
SfcI CTRYAG 2 cut(s) 1114, 1148
Sfr274I CTCGAG 1 cut(s) 921
SfuI TTCGAA 2 cut(s) 735, 1502
SinI GGWCC 9 cut(s) 978, 1352, 1520, 1544, 1568, 1592, 1616, 1640, 1805
SlaI CTCGAG 1 cut(s) 921
SmiMI CAYNNNNRTG 1 cut(s) 776
SmlI CTYRAG 2 cut(s) 921, 1275
SmoI CTYRAG 2 cut(s) 921, 1275
SsiI CCGC 7 cut(s) 324, 1073, 1542, 1566, 1614, 1673, 1853
SspMI CTAG 2 cut(s) 1659, 1914
SstI GAGCTC 1 cut(s) 728
StyD4I CCNGG 7 cut(s) 306, 877, 1354, 1570, 1594, 1718, 1780
StyI CCWWGG 2 cut(s) 1059, 1380
TaaI ACNGT 2 cut(s) 389, 542
TaiI ACGT 2 cut(s) 249, 318
TaqI TCGA 6 cut(s) 466, 735, 819, 922, 1487, 1502
TatI WGTACW 1 cut(s) 996
TfiI GAWTC 3 cut(s) 303, 660, 1076
Tru1I TTAA 3 cut(s) 125, 537, 654
Tru9I TTAA 3 cut(s) 125, 537, 654
TscAI CASTG 1 cut(s) 394
TseFI GTSAC 1 cut(s) 526
TseI GCWGC 6 cut(s) 74, 333, 367, 472, 701, 1844
Tsp45I GTSAC 1 cut(s) 526
TspDTI ATGAA 6 cut(s) 101, 182, 214, 432, 609, 1472
TspGWI ACGGA 3 cut(s) 394, 500, 844
TspRI CASTG 1 cut(s) 394
Van91I CCANNNNNTGG 3 cut(s) 1589, 1637, 1781
VpaK11BI GGWCC 9 cut(s) 978, 1352, 1520, 1544, 1568, 1592, 1616, 1640, 1805
XapI RAATTY 4 cut(s) 1201, 1210, 1261, 1459
XbaI TCTAGA 1 cut(s) 1658
XhoI CTCGAG 1 cut(s) 921
XmiI GTMKAC 1 cut(s) 785
XspI CTAG 2 cut(s) 1659, 1914
ZrmI AGTACT 1 cut(s) 998
Zsp2I ATGCAT 1 cut(s) 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.