Rorug01G0042900

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0014972
Physical Location & Seq
Reverse (-)
7134758 .. 7135474
717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0042900.1

Sequence Viewer

Length: 717 bp
ATGTCCACCATCGACTCTGTAACCAAAAGTTTTGCCACCTCGCTTGCCATTGCAAGCAATGAAGTTGTTGATATCTCCCGTATGTCTGAGGGAGGAGCGCGTCGCAATTCCCAATCCTACCTTTTGGCATGGCCTTTGGCTATGAAGGCAACCTCGGCTGTTGATCTGCAGAGGCATTTTCGCCGAATCTGGATTCTGAAGCAGAGTTTTAGCATTCAACAGCGAGCACCAGACTGCTTTCTATTCTCATTTGATCTCAAAGGGGATCGTAACAAGGTCTTGATGGGGGGACCTTGGAGTTTTGGGAGGGCAATGGTGGTGGTTCAGCCCTATGATGGAGTTGCTCCGATGCAGGCGGTACCAATGCGTGATCTTCTCTGTTGGGTACGCATCTCCAATATGCCACCGGCGTATGAAGAAAAAGAAACAATTGTCGATGTCGCATCGGTGGTAGGGAATTTCCTTGATCTGGACCGAAAGCTGTTTGATACAATGAGGGAGATTAGGGTTCGAGTGTCTCACCCTATCTCGAAACCATTCTTCAAGGAGAAAACCCTAAAGTTTGCGCAGGATGTGGAACAAGAAGTCCATTTCTTCTTTGAGAAGCTAGTGGGACTGTGCAACACCTGCGGGCGGGTGTTTCATGAAGGTGGAACATGTGATGTTGTCTCTCCACCTTTGACAAAGAAGAAGGATGTTGAAAACCCGGTGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

238

Amino Acids

26.9

Weight (kDa)

8.8

Isoelectric Point (pI)

39.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 55 - 112 1.3e-09 Domain of unknown function (DUF4283)
zf-CCHC_4 PF14392 176 - 221 2e-07 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40815 AT2G40815
fragaria_vesca FvH4_7g26311
malus_domestica MD01G1172000.v1.1 MD07G1238500.v1.1
prunus_persica Prupe.2G267300_v2.0.a1
pyrus_communis pycom07g21450
rosa_chinensis RchiOBHm_Chr1g0372771
rosa_laevigata RLG00000026873
rosa_multiflora Rmu_sc0029212.1_g000001
rosa_rugosa Rorug01G0042900 Rorug01G0374300
rosa_samantha Rh1AG383000 Rh1CG359900 Rh1DG377600
rosa_wichuraiana Rw1G033710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 635
Acc16I TGCGCA 1 cut(s) 567
Acc36I ACCTGC 1 cut(s) 635
Acc65I GGTACC 1 cut(s) 358
AccB1I GGYRCC 1 cut(s) 358
AccII CGCG 1 cut(s) 100
AciI CCGC 3 cut(s) 356, 630, 634
AclWI GGATC 1 cut(s) 273
AcsI RAATTY 1 cut(s) 457
AcuI CTGAAG 1 cut(s) 218
AfaI GTAC 2 cut(s) 360, 387
AfiI CCNNNNNNNGG 3 cut(s) 335, 469, 633
AflIII ACRYGT 1 cut(s) 656
AgsI TTSAA 3 cut(s) 218, 544, 701
AloI GAACNNNNNNTCC 2 cut(s) 570, 602
AluBI AGCT 2 cut(s) 481, 607
AluI AGCT 2 cut(s) 481, 607
Alw21I GWGCWC 1 cut(s) 229
Alw26I GTCTC 2 cut(s) 522, 673
AlwI GGATC 1 cut(s) 273
AoxI GGCC 2 cut(s) 131, 711
ApoI RAATTY 1 cut(s) 457
Asp700I GAANNNNTTC 1 cut(s) 536
Asp718I GGTACC 1 cut(s) 358
AspLEI GCGC 2 cut(s) 100, 568
AspS9I GGNCC 2 cut(s) 290, 472
AsuC2I CCSGG 1 cut(s) 707
AsuHPI GGTGA 1 cut(s) 512
AvaII GGWCC 2 cut(s) 290, 472
BanI GGYRCC 1 cut(s) 358
Bbv12I GWGCWC 1 cut(s) 229
BccI CCATC 3 cut(s) 17, 277, 329
BcnI CCSGG 1 cut(s) 707
BcoDI GTCTC 2 cut(s) 522, 673
BfaI CTAG 1 cut(s) 608
BfmI CTRYAG 1 cut(s) 167
BfuAI ACCTGC 1 cut(s) 635
Bme1390I CCNGG 1 cut(s) 707
Bme18I GGWCC 2 cut(s) 290, 472
BmgT120I GGNCC 2 cut(s) 290, 472
BmiI GGNNCC 2 cut(s) 291, 360
BmrFI CCNGG 1 cut(s) 707
BmsI GCATC 3 cut(s) 339, 399, 452
BpuMI CCSGG 1 cut(s) 707
BsaJI CCNNGG 2 cut(s) 153, 293
Bsc4I CCNNNNNNNGG 3 cut(s) 335, 469, 633
Bse118I RCCGGY 1 cut(s) 406
Bse3DI GCAATG 3 cut(s) 48, 64, 318
BseDI CCNNGG 2 cut(s) 153, 293
BseGI GGATG 2 cut(s) 577, 700
BseLI CCNNNNNNNGG 3 cut(s) 335, 469, 633
BseMI GCAATG 3 cut(s) 48, 64, 318
BseMII CTCAG 1 cut(s) 78
BseRI GAGGAG 1 cut(s) 108
Bsh1236I CGCG 1 cut(s) 100
BshFI GGCC 2 cut(s) 133, 713
BshNI GGYRCC 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 229
BsiSI CCGG 2 cut(s) 407, 707
BslFI GGGAC 2 cut(s) 303, 627
BslI CCNNNNNNNGG 3 cut(s) 335, 469, 633
BsmAI GTCTC 2 cut(s) 522, 673
BsmFI GGGAC 2 cut(s) 303, 627
BsmI GAATGC 1 cut(s) 213
BsnI GGCC 2 cut(s) 133, 713
Bsp1286I GDGCHC 1 cut(s) 229
Bsp143I GATC 5 cut(s) 163, 253, 265, 370, 466
BspACI CCGC 3 cut(s) 356, 630, 634
BspANI GGCC 2 cut(s) 133, 713
BspCNI CTCAG 1 cut(s) 79
BspFNI CGCG 1 cut(s) 100
BspHI TCATGA 1 cut(s) 643
BspLI GGNNCC 2 cut(s) 291, 360
BspMAI CTGCAG 1 cut(s) 171
BspMI ACCTGC 1 cut(s) 635
BspPI GGATC 1 cut(s) 273
BspT107I GGYRCC 1 cut(s) 358
BsrDI GCAATG 3 cut(s) 48, 64, 318
BsrFI RCCGGY 1 cut(s) 406
BssAI RCCGGY 1 cut(s) 406
BssECI CCNNGG 2 cut(s) 153, 293
BssMI GATC 5 cut(s) 163, 253, 265, 370, 466
BssT1I CCWWGG 1 cut(s) 293
Bst4CI ACNGT 1 cut(s) 618
BstAPI GCANNNNNTGC 1 cut(s) 627
BstC8I GCNNGC 5 cut(s) 45, 55, 225, 354, 632
BstDEI CTNAG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 577, 700
BstFNI CGCG 1 cut(s) 100
BstHHI GCGC 2 cut(s) 100, 568
BstKTI GATC 5 cut(s) 166, 256, 268, 373, 469
BstMAI GTCTC 2 cut(s) 522, 673
BstMBI GATC 5 cut(s) 163, 253, 265, 370, 466
BstMWI GCNNNNNNNGC 3 cut(s) 146, 155, 627
BstNSI RCATGY 1 cut(s) 660
BstSCI CCNGG 1 cut(s) 705
BstSFI CTRYAG 1 cut(s) 167
BstUI CGCG 1 cut(s) 100
BsuRI GGCC 2 cut(s) 133, 713
BtsCI GGATG 2 cut(s) 577, 700
BveI ACCTGC 1 cut(s) 635
Cac8I GCNNGC 5 cut(s) 45, 55, 225, 354, 632
CciI TCATGA 1 cut(s) 643
CfoI GCGC 2 cut(s) 100, 568
Cfr10I RCCGGY 1 cut(s) 406
Cfr13I GGNCC 2 cut(s) 290, 472
CseI GACGC 1 cut(s) 89
Csp6I GTAC 2 cut(s) 359, 386
CspCI CAANNNNNGTGG 4 cut(s) 25, 60, 300, 335
CviAII CATG 3 cut(s) 129, 644, 657
CviJI RGCY 7 cut(s) 133, 140, 158, 328, 481, 607, 713
CviKI_1 RGCY 7 cut(s) 133, 140, 158, 328, 481, 607, 713
CviQI GTAC 2 cut(s) 359, 386
DdeI CTNAG 1 cut(s) 87
DpnI GATC 5 cut(s) 165, 255, 267, 372, 468
DpnII GATC 5 cut(s) 163, 253, 265, 370, 466
Eco130I CCWWGG 1 cut(s) 293
Eco32I GATATC 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 290, 472
Eco57I CTGAAG 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 290
EcoRV GATATC 1 cut(s) 73
EcoT14I CCWWGG 1 cut(s) 293
ErhI CCWWGG 1 cut(s) 293
FaeI CATG 3 cut(s) 132, 647, 660
FaiI YATR 8 cut(s) 83, 130, 143, 333, 401, 414, 645, 658
FaqI GGGAC 2 cut(s) 303, 627
FatI CATG 3 cut(s) 128, 643, 656
FauI CCCGC 2 cut(s) 623, 627
FokI GGATG 2 cut(s) 584, 707
FspBI CTAG 1 cut(s) 608
FspI TGCGCA 1 cut(s) 567
GlaI GCGC 2 cut(s) 99, 567
HaeIII GGCC 2 cut(s) 133, 713
HapII CCGG 2 cut(s) 407, 707
HgaI GACGC 1 cut(s) 89
HhaI GCGC 2 cut(s) 100, 568
Hin1II CATG 3 cut(s) 132, 647, 660
Hin6I GCGC 2 cut(s) 98, 566
HinP1I GCGC 2 cut(s) 98, 566
HinfI GANTC 3 cut(s) 14, 186, 193
HpaII CCGG 2 cut(s) 407, 707
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 3 cut(s) 88, 198, 348
Hpy188III TCNNGA 5 cut(s) 190, 280, 470, 529, 644
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 1 cut(s) 105
HpyAV CCTTC 3 cut(s) 139, 641, 685
HpyCH4III ACNGT 1 cut(s) 618
HpyCH4V TGCA 4 cut(s) 53, 169, 352, 621
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 155, 627
HpyF3I CTNAG 1 cut(s) 87
Hsp92II CATG 3 cut(s) 132, 647, 660
HspAI GCGC 2 cut(s) 98, 566
KpnI GGTACC 1 cut(s) 362
Kzo9I GATC 5 cut(s) 163, 253, 265, 370, 466
LmnI GCTCC 2 cut(s) 95, 349
LpnPI CCDG 7 cut(s) 175, 243, 338, 420, 455, 554, 640
LweI GCATC 3 cut(s) 339, 399, 452
MaeI CTAG 1 cut(s) 608
MaeIII GTNAC 2 cut(s) 19, 269
MalI GATC 5 cut(s) 165, 255, 267, 372, 468
MboI GATC 5 cut(s) 163, 253, 265, 370, 466
MboII GAAGA 5 cut(s) 365, 428, 532, 586, 700
MfeI CAATTG 1 cut(s) 429
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 3 cut(s) 106, 429, 457
MlyI GAGTC 1 cut(s) 8
MnlI CCTC 7 cut(s) 49, 82, 86, 163, 165, 300, 489
MroXI GAANNNNTTC 1 cut(s) 536
MslI CAYNNNNRTG 1 cut(s) 648
MspI CCGG 2 cut(s) 407, 707
MspR9I CCNGG 1 cut(s) 707
MunI CAATTG 1 cut(s) 429
Mva1269I GAATGC 1 cut(s) 213
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 3 cut(s) 146, 155, 627
NciI CCSGG 1 cut(s) 707
NdeII GATC 5 cut(s) 163, 253, 265, 370, 466
NlaIII CATG 3 cut(s) 132, 647, 660
NlaIV GGNNCC 2 cut(s) 291, 360
NmeAIII GCCGAG 1 cut(s) 134
NsbI TGCGCA 1 cut(s) 567
NspI RCATGY 1 cut(s) 660
PagI TCATGA 1 cut(s) 643
PaqCI CACCTGC 1 cut(s) 635
PciI ACATGT 1 cut(s) 656
PctI GAATGC 1 cut(s) 213
PdmI GAANNNNTTC 1 cut(s) 536
PfeI GAWTC 2 cut(s) 186, 193
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
PpuMI RGGWCCY 1 cut(s) 290
PscI ACATGT 1 cut(s) 656
Psp5II RGGWCCY 1 cut(s) 290
PspN4I GGNNCC 2 cut(s) 291, 360
PspPI GGNCC 2 cut(s) 290, 472
PspPPI RGGWCCY 1 cut(s) 290
PstI CTGCAG 1 cut(s) 171
RsaI GTAC 2 cut(s) 360, 387
RsaNI GTAC 2 cut(s) 359, 386
RseI CAYNNNNRTG 1 cut(s) 648
Sau3AI GATC 5 cut(s) 163, 253, 265, 370, 466
Sau96I GGNCC 2 cut(s) 290, 472
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 1 cut(s) 707
SduI GDGCHC 1 cut(s) 229
SfaNI GCATC 3 cut(s) 339, 399, 452
SfcI CTRYAG 1 cut(s) 167
SgrAI CRCCGGYG 1 cut(s) 406
SinI GGWCC 2 cut(s) 290, 472
SmiMI CAYNNNNRTG 1 cut(s) 648
Sse9I AATT 3 cut(s) 106, 429, 457
SsiI CCGC 3 cut(s) 356, 630, 634
SspMI CTAG 1 cut(s) 608
StyD4I CCNGG 1 cut(s) 705
StyI CCWWGG 1 cut(s) 293
TaaI ACNGT 1 cut(s) 618
TaqI TCGA 4 cut(s) 12, 435, 511, 530
TaqII GACCGA 1 cut(s) 489
TasI AATT 3 cut(s) 106, 429, 457
TfiI GAWTC 2 cut(s) 186, 193
TspDTI ATGAA 5 cut(s) 75, 158, 429, 632, 660
VpaK11BI GGWCC 2 cut(s) 290, 472
XapI RAATTY 1 cut(s) 457
XceI RCATGY 1 cut(s) 660
XmnI GAANNNNTTC 1 cut(s) 536
XspI CTAG 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.