pycom07g21450

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0014972
Physical Location & Seq
Reverse (-)
23158520 .. 23159635
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g21450.2

Sequence Viewer

Length: 1026 bp
ATGGGGAAACTCTGGGTTGAAGTGTGCCTGATATCCGCTCGAGGGCTCACGCGTTCCTCTTCGTTATGGAAGCTCCAGTGGTATGCTGTTGGGTGGACCAATCCCAACAACAAATACTGCACCAAGATTGATGCATCTGGAAATGCAAATCCGGTTTGGAGAACCAAGTTTGCTACCTTGGTTGAGGGCTCGGAGGCGGACTTGCAGGATCTGGCACTGCATATTGAAGTGTACAGCAGAGAGCCTATATTCCTCAGAGAAAAGCTTCAGGGGACGGCAACTATTGTCCTGAAAGAGTTTCTGTCTAAGCATAACAAGAACTCTGGGGATCCAAGGCAAGGAGCTGAAGAAGTCGGGAGCTACCAGTTGCGGAAGAAGAACTCCAACAAACCTCAAGGGTTCGTCGATGTTTCGATACGGATTTCCGAGGATATGGAAGCTCAAAGCTCGTACACAGGCAGTGAGGGAGGACCTATGGATCACAGCAATACCATCACCTTGGCTACTGGAGATGGGTCTGCACCAGCGATACGACCTGTAGCTCGACAACAAAGGCCAGAAGATCAGTTCCGTATCAACTCTCCATATGCACAACCAATGCCTTTCCCTCCGAACTATTCCAATCCTTCTGCAGTTGGACCAAGCTACCCACCAGCCAGCGCCCCAAGTTACCCACCAGCAAACGGACCAAACTACCCTTCAGCAAGCGGACCAAGCTACCCACCAGCAGGCGGGCCAAGCTATCAGCCGCCTAGAACTCCACCACCGCCTCCCCCACCTTCTAATGTTGGCTACGTACCCACTTTTGTCCGGAGAACAGATCGAATGCCAGAGACCTATATTAATATGCCATCATCTGGAGCACCGCCTGGTCGCAGAGGAGCAGCTGGTGTTGGAATGGGAATCGGTGCTGGAGCGCTGGCGGCTGGTGCTGTGATCTTTGGTGATGACTTCATGTCAGGATTCGACGTTCCTTCTGGCTTACAAGATGCTAGTCTTACTATATCAATTGATCCTCCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

342

Amino Acids

36.48

Weight (kDa)

7.71

Isoelectric Point (pI)

62.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40815 AT2G40815
fragaria_vesca FvH4_7g26311
malus_domestica MD01G1172000.v1.1 MD07G1238500.v1.1
prunus_persica Prupe.2G267300_v2.0.a1
pyrus_communis pycom07g21450
rosa_chinensis RchiOBHm_Chr1g0372771
rosa_laevigata RLG00000026873
rosa_multiflora Rmu_sc0029212.1_g000001
rosa_rugosa Rorug01G0042900 Rorug01G0374300
rosa_samantha Rh1AG383000 Rh1CG359900 Rh1DG377600
rosa_wichuraiana Rw1G033710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 857
AccBSI CCGCTC 1 cut(s) 38
AccII CGCG 1 cut(s) 52
AccIII TCCGGA 1 cut(s) 810
AciI CCGC 9 cut(s) 36, 197, 370, 708, 732, 749, 767, 866, 923
AclWI GGATC 5 cut(s) 216, 323, 336, 486, 1007
AcuI CTGAAG 3 cut(s) 251, 366, 684
AfaI GTAC 3 cut(s) 233, 452, 798
AfeI AGCGCT 1 cut(s) 918
AfiI CCNNNNNNNGG 6 cut(s) 42, 338, 683, 728, 731, 857
AflIII ACRYGT 1 cut(s) 50
AgsI TTSAA 2 cut(s) 20, 227
AhdI GACNNNNNGTC 1 cut(s) 955
AjnI CCWGG 1 cut(s) 868
AjuI GAANNNNNNNTTGG 2 cut(s) 682, 714
AloI GAACNNNNNNTCC 2 cut(s) 954, 986
Alw21I GWGCWC 1 cut(s) 865
Alw26I GTCTC 1 cut(s) 827
AlwI GGATC 5 cut(s) 216, 323, 336, 486, 1007
AlwNI CAGNNNCTG 1 cut(s) 211
Ama87I CYCGRG 1 cut(s) 39
Aor13HI TCCGGA 1 cut(s) 810
Aor51HI AGCGCT 1 cut(s) 918
AoxI GGCC 2 cut(s) 554, 734
ApeKI GCWGC 1 cut(s) 884
AseI ATTAAT 1 cut(s) 843
Asp700I GAANNNNTTC 1 cut(s) 264
AspLEI GCGC 2 cut(s) 662, 919
AspS9I GGNCC 6 cut(s) 96, 470, 638, 686, 710, 734
AsuHPI GGTGA 2 cut(s) 487, 956
AvaI CYCGRG 1 cut(s) 39
AvaII GGWCC 5 cut(s) 96, 470, 638, 686, 710
BamHI GGATCC 1 cut(s) 328
BanII GRGCYC 2 cut(s) 48, 191
Bbv12I GWGCWC 1 cut(s) 865
BbvI GCAGC 1 cut(s) 896
BccI CCATC 3 cut(s) 500, 506, 859
BceAI ACGGC 1 cut(s) 291
BciT130I CCWGG 1 cut(s) 870
BcoDI GTCTC 1 cut(s) 827
BfaI CTAG 2 cut(s) 753, 993
BfmI CTRYAG 2 cut(s) 537, 630
BfoI RGCGCY 2 cut(s) 663, 920
BisI GCNGC 3 cut(s) 749, 885, 924
BlsI GCNGC 3 cut(s) 750, 886, 925
Bme1390I CCNGG 1 cut(s) 870
Bme18I GGWCC 5 cut(s) 96, 470, 638, 686, 710
BmeRI GACNNNNNGTC 1 cut(s) 955
BmeT110I CYCGRG 1 cut(s) 39
BmgT120I GGNCC 6 cut(s) 96, 470, 638, 686, 710, 734
BmiI GGNNCC 1 cut(s) 330
BmrFI CCNGG 1 cut(s) 870
BmsI GCATC 3 cut(s) 121, 143, 979
BpmI CTGGAG 4 cut(s) 59, 528, 879, 933
BpuEI CTTGAG 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 796
BsaI GGTCTC 1 cut(s) 827
BsaJI CCNNGG 4 cut(s) 177, 332, 426, 498
BsaWI WCCGGW 2 cut(s) 151, 810
Bsc4I CCNNNNNNNGG 6 cut(s) 42, 338, 683, 728, 731, 857
Bse1I ACTGG 3 cut(s) 76, 364, 511
BseAI TCCGGA 1 cut(s) 810
BseBI CCWGG 1 cut(s) 870
BseDI CCNNGG 4 cut(s) 177, 332, 426, 498
BseLI CCNNNNNNNGG 6 cut(s) 42, 338, 683, 728, 731, 857
BseMII CTCAG 1 cut(s) 268
BseNI ACTGG 3 cut(s) 76, 364, 511
BseRI GAGGAG 1 cut(s) 894
BseXI GCAGC 1 cut(s) 896
BsgI GTGCAG 2 cut(s) 103, 504
Bsh1236I CGCG 1 cut(s) 52
BshFI GGCC 2 cut(s) 556, 736
BsiHKAI GWGCWC 1 cut(s) 865
BsiHKCI CYCGRG 1 cut(s) 39
BsiSI CCGG 2 cut(s) 152, 811
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 6 cut(s) 42, 338, 683, 728, 731, 857
BsmAI GTCTC 1 cut(s) 827
BsmFI GGGAC 1 cut(s) 286
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 2 cut(s) 556, 736
Bso31I GGTCTC 1 cut(s) 827
BsoBI CYCGRG 1 cut(s) 39
Bsp1286I GDGCHC 3 cut(s) 48, 191, 865
Bsp13I TCCGGA 1 cut(s) 810
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 7 cut(s) 208, 328, 478, 562, 820, 936, 1012
BspACI CCGC 9 cut(s) 36, 197, 370, 708, 732, 749, 767, 866, 923
BspANI GGCC 2 cut(s) 556, 736
BspCNI CTCAG 1 cut(s) 267
BspEI TCCGGA 1 cut(s) 810
BspFNI CGCG 1 cut(s) 52
BspLI GGNNCC 1 cut(s) 330
BspMAI CTGCAG 1 cut(s) 634
BspPI GGATC 5 cut(s) 216, 323, 336, 486, 1007
BspTNI GGTCTC 1 cut(s) 827
BsrBI CCGCTC 1 cut(s) 38
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 3 cut(s) 76, 364, 511
BssECI CCNNGG 4 cut(s) 177, 332, 426, 498
BssMI GATC 7 cut(s) 208, 328, 478, 562, 820, 936, 1012
BssT1I CCWWGG 3 cut(s) 177, 332, 498
Bst2UI CCWGG 1 cut(s) 870
Bst6I CTCTTC 1 cut(s) 64
BstAUI TGTACA 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 796
BstC8I GCNNGC 5 cut(s) 658, 706, 730, 734, 921
BstDEI CTNAG 2 cut(s) 254, 306
BstFNI CGCG 1 cut(s) 52
BstH2I RGCGCY 2 cut(s) 663, 920
BstHHI GCGC 2 cut(s) 662, 919
BstKTI GATC 7 cut(s) 211, 331, 481, 565, 823, 939, 1015
BstMAI GTCTC 1 cut(s) 827
BstMBI GATC 7 cut(s) 208, 328, 478, 562, 820, 936, 1012
BstMWI GCNNNNNNNGC 4 cut(s) 714, 738, 923, 929
BstNI CCWGG 1 cut(s) 870
BstSCI CCNGG 1 cut(s) 868
BstSFI CTRYAG 2 cut(s) 537, 630
BstSNI TACGTA 1 cut(s) 796
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 1 cut(s) 896
BstX2I RGATCY 2 cut(s) 208, 328
BstXI CCANNNNNNTGG 1 cut(s) 499
BstYI RGATCY 2 cut(s) 208, 328
BsuRI GGCC 2 cut(s) 556, 736
BtsI GCAGTG 2 cut(s) 215, 466
BtsIMutI CAGTG 3 cut(s) 83, 215, 466
Cac8I GCNNGC 5 cut(s) 658, 706, 730, 734, 921
CaiI CAGNNNCTG 1 cut(s) 211
CfoI GCGC 2 cut(s) 662, 919
Cfr13I GGNCC 6 cut(s) 96, 470, 638, 686, 710, 734
Csp6I GTAC 3 cut(s) 232, 451, 797
CviAII CATG 1 cut(s) 955
CviQI GTAC 3 cut(s) 232, 451, 797
DdeI CTNAG 2 cut(s) 254, 306
DpnI GATC 7 cut(s) 210, 330, 480, 564, 822, 938, 1014
DpnII GATC 7 cut(s) 208, 328, 478, 562, 820, 936, 1012
DriI GACNNNNNGTC 1 cut(s) 955
Eam1104I CTCTTC 1 cut(s) 64
Eam1105I GACNNNNNGTC 1 cut(s) 955
EarI CTCTTC 1 cut(s) 64
EciI GGCGGA 1 cut(s) 212
Eco105I TACGTA 1 cut(s) 796
Eco130I CCWWGG 3 cut(s) 177, 332, 498
Eco24I GRGCYC 2 cut(s) 48, 191
Eco31I GGTCTC 1 cut(s) 827
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 5 cut(s) 96, 470, 638, 686, 710
Eco47III AGCGCT 1 cut(s) 918
Eco57I CTGAAG 3 cut(s) 251, 366, 684
Eco88I CYCGRG 1 cut(s) 39
EcoO109I RGGNCCY 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 868
EcoRV GATATC 1 cut(s) 33
EcoT14I CCWWGG 3 cut(s) 177, 332, 498
EcoT22I ATGCAT 1 cut(s) 136
EcoT38I GRGCYC 2 cut(s) 48, 191
ErhI CCWWGG 3 cut(s) 177, 332, 498
FaeI CATG 1 cut(s) 958
FaqI GGGAC 1 cut(s) 286
FatI CATG 1 cut(s) 954
FauI CCCGC 1 cut(s) 725
FauNDI CATATG 1 cut(s) 586
Fnu4HI GCNGC 3 cut(s) 749, 885, 924
FriOI GRGCYC 2 cut(s) 48, 191
Fsp4HI GCNGC 3 cut(s) 749, 885, 924
FspBI CTAG 2 cut(s) 753, 993
GlaI GCGC 2 cut(s) 661, 918
GluI GCNGC 3 cut(s) 749, 885, 924
GsuI CTGGAG 4 cut(s) 59, 528, 879, 933
HaeII RGCGCY 2 cut(s) 663, 920
HaeIII GGCC 2 cut(s) 556, 736
HapII CCGG 2 cut(s) 152, 811
HhaI GCGC 2 cut(s) 662, 919
Hin1II CATG 1 cut(s) 958
Hin6I GCGC 2 cut(s) 660, 917
HinP1I GCGC 2 cut(s) 660, 917
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 2 cut(s) 903, 963
HpaII CCGG 2 cut(s) 152, 811
HphI GGTGA 2 cut(s) 487, 956
Hpy166II GTNNAC 3 cut(s) 96, 232, 453
Hpy188I TCNGA 5 cut(s) 193, 257, 427, 612, 1025
Hpy188III TCNNGA 6 cut(s) 138, 289, 355, 811, 858, 960
Hpy8I GTNNAC 3 cut(s) 96, 232, 453
Hpy99I CGWCG 2 cut(s) 407, 971
HpyAV CCTTC 4 cut(s) 636, 708, 789, 984
HpyCH4IV ACGT 2 cut(s) 795, 969
HpyCH4V TGCA 8 cut(s) 120, 134, 146, 205, 220, 521, 590, 632
HpyF10VI GCNNNNNNNGC 4 cut(s) 714, 738, 923, 929
HpyF3I CTNAG 2 cut(s) 254, 306
HpySE526I ACGT 2 cut(s) 795, 969
Hsp92II CATG 1 cut(s) 958
HspAI GCGC 2 cut(s) 660, 917
Kpn2I TCCGGA 1 cut(s) 810
Kzo9I GATC 7 cut(s) 208, 328, 478, 562, 820, 936, 1012
LmnI GCTCC 6 cut(s) 78, 341, 357, 860, 881, 914
Lsp1109I GCAGC 1 cut(s) 896
LweI GCATC 3 cut(s) 121, 143, 979
MaeI CTAG 2 cut(s) 753, 993
MaeII ACGT 2 cut(s) 795, 969
MaeIII GTNAC 1 cut(s) 668
MalI GATC 7 cut(s) 210, 330, 480, 564, 822, 938, 1014
MbiI CCGCTC 1 cut(s) 38
MboI GATC 7 cut(s) 208, 328, 478, 562, 820, 936, 1012
MboII GAAGA 5 cut(s) 51, 359, 385, 388, 572
MfeI CAATTG 1 cut(s) 1008
MflI RGATCY 2 cut(s) 208, 328
MhlI GDGCHC 3 cut(s) 48, 191, 865
MluCI AATT 1 cut(s) 1008
MluI ACGCGT 1 cut(s) 50
MmeI TCCRAC 3 cut(s) 408, 616, 874
Mph1103I ATGCAT 1 cut(s) 136
MroI TCCGGA 1 cut(s) 810
MroXI GAANNNNTTC 1 cut(s) 264
MseI TTAA 1 cut(s) 843
MspA1I CMGCKG 1 cut(s) 887
MspI CCGG 2 cut(s) 152, 811
MspR9I CCNGG 1 cut(s) 870
MunI CAATTG 1 cut(s) 1008
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 1 cut(s) 870
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 4 cut(s) 714, 738, 923, 929
NdeI CATATG 1 cut(s) 586
NdeII GATC 7 cut(s) 208, 328, 478, 562, 820, 936, 1012
NlaIII CATG 1 cut(s) 958
NlaIV GGNNCC 1 cut(s) 330
NsiI ATGCAT 1 cut(s) 136
PaeR7I CTCGAG 1 cut(s) 39
PctI GAATGC 1 cut(s) 831
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 2 cut(s) 903, 963
PflMI CCANNNNNTGG 1 cut(s) 857
PkrI GCNGC 3 cut(s) 750, 886, 925
Ppu21I YACGTR 1 cut(s) 796
PpuMI RGGWCCY 1 cut(s) 470
PshBI ATTAAT 1 cut(s) 843
Psp5II RGGWCCY 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 868
PspGI CCWGG 1 cut(s) 868
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 6 cut(s) 96, 470, 638, 686, 710, 734
PspPPI RGGWCCY 1 cut(s) 470
PspXI VCTCGAGB 1 cut(s) 39
PstI CTGCAG 1 cut(s) 634
PstNI CAGNNNCTG 1 cut(s) 211
PsuI RGATCY 2 cut(s) 208, 328
PvuII CAGCTG 1 cut(s) 887
RsaI GTAC 3 cut(s) 233, 452, 798
RsaNI GTAC 3 cut(s) 232, 451, 797
SaqAI TTAA 1 cut(s) 843
SatI GCNGC 3 cut(s) 749, 885, 924
Sau3AI GATC 7 cut(s) 208, 328, 478, 562, 820, 936, 1012
Sau96I GGNCC 6 cut(s) 96, 470, 638, 686, 710, 734
ScrFI CCNGG 1 cut(s) 870
SduI GDGCHC 3 cut(s) 48, 191, 865
SfaNI GCATC 3 cut(s) 121, 143, 979
SfcI CTRYAG 2 cut(s) 537, 630
Sfr274I CTCGAG 1 cut(s) 39
SinI GGWCC 5 cut(s) 96, 470, 638, 686, 710
SlaI CTCGAG 1 cut(s) 39
SmlI CTYRAG 2 cut(s) 39, 393
SmoI CTYRAG 2 cut(s) 39, 393
SnaBI TACGTA 1 cut(s) 796
Sse9I AATT 1 cut(s) 1008
SsiI CCGC 9 cut(s) 36, 197, 370, 708, 732, 749, 767, 866, 923
SspMI CTAG 2 cut(s) 753, 993
StyD4I CCNGG 1 cut(s) 868
StyI CCWWGG 3 cut(s) 177, 332, 498
TaiI ACGT 2 cut(s) 798, 972
TaqI TCGA 6 cut(s) 40, 405, 413, 544, 823, 966
TasI AATT 1 cut(s) 1008
TatI WGTACW 1 cut(s) 231
TauI GCSGC 2 cut(s) 751, 926
TfiI GAWTC 2 cut(s) 903, 963
Tru1I TTAA 1 cut(s) 843
Tru9I TTAA 1 cut(s) 843
TscAI CASTG 3 cut(s) 83, 222, 466
TseI GCWGC 1 cut(s) 884
TspDTI ATGAA 1 cut(s) 943
TspGWI ACGGA 3 cut(s) 433, 560, 699
TspRI CASTG 3 cut(s) 83, 222, 466
Van91I CCANNNNNTGG 1 cut(s) 857
VpaK11BI GGWCC 5 cut(s) 96, 470, 638, 686, 710
VspI ATTAAT 1 cut(s) 843
XhoI CTCGAG 1 cut(s) 39
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 2 cut(s) 753, 993
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.