Rmu_sc0029212.1_g000001

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0029212.1
OG0014972
Physical Location & Seq
Forward (+)
187 .. 1396
1210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0029212.1_g000001.1.cds

Sequence Viewer

Length: 1065 bp
atggggaaactctgggtagaagtttgcttgatatctgctcgagggctccggcgttcgtcgtccctatggaagcttcaatggtatgctgttgggtggaccaatcccaataacaagtactgcaccaagattgatgcatctggaaatgcaaatccagtatggaaaaccaagtttgctaccttggttgaggacgcggaatctaaactgaatgatttggctctgcacattgaagtctatagtagagagcctatattcctcagagaaaggctacaggggacagctactattctcttgaaagagtttctggctaagcataacaagaattctgagaattcaaggcatggagttgaagaagttgggagctaccaattgcgaaaaaagaattccaacaaacctcaaggttttgttgatgtttcaatccacatttctgaggatatggaagggagaagctcatacacaggaaatgagggaggacctggggatcacagcaatactatcaccttggctactggagatggatctgcaccagcattactaccatttactccacaaaagagaccagaacatcaattccaaatgaattccccctatgcaaatccaatgccttcgactccatactattcgaaaccatctgctgttggaccaagttacacaccagcaagcggaccaagctacacgccagcaagcggaccaagcaacctgccagcaaatggaccaggctacctgccagcaagcggactaggctacctgccagcaagtggaccaagctatcagccttctagaactcctccaccgcctccacccccgtccaatgttggatatgtacctacttttctccccaggacagatcgaatgactgacacctatatcaatatgccctcatcatctggagcaccccctcctggtcgtggaactcgggccggaggacctgcttttgcaatgggaatgggtgctggagcgctggcagccggtgcggtgatcttcggtgatgactttatgtcaggatttgatgttccttcaggcttacaagatgctagtgttaccatatcagctgatcctcctttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

354

Amino Acids

37.73

Weight (kDa)

8.37

Isoelectric Point (pI)

58.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40815 AT2G40815
fragaria_vesca FvH4_7g26311
malus_domestica MD01G1172000.v1.1 MD07G1238500.v1.1
prunus_persica Prupe.2G267300_v2.0.a1
pyrus_communis pycom07g21450
rosa_chinensis RchiOBHm_Chr1g0372771
rosa_laevigata RLG00000026873
rosa_multiflora Rmu_sc0029212.1_g000001
rosa_rugosa Rorug01G0042900 Rorug01G0374300
rosa_samantha Rh1AG383000 Rh1CG359900 Rh1DG377600
rosa_wichuraiana Rw1G033710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 705, 729, 753, 934
AccB7I CCANNNNNTGG 2 cut(s) 707, 755
AccII CGCG 1 cut(s) 191
AciI CCGC 6 cut(s) 191, 660, 684, 732, 791, 971
AclWI GGATC 3 cut(s) 486, 523, 1046
AcsI RAATTY 4 cut(s) 319, 328, 379, 577
AcuI CTGAAG 1 cut(s) 999
AfaI GTAC 2 cut(s) 116, 822
AfeI AGCGCT 1 cut(s) 957
AfiI CCNNNNNNNGG 7 cut(s) 659, 683, 707, 731, 755, 899, 905
AgsI TTSAA 6 cut(s) 77, 227, 292, 333, 347, 414
AhdI GACNNNNNGTC 1 cut(s) 994
AjnI CCWGG 4 cut(s) 472, 712, 836, 898
AloI GAACNNNNNNTCC 4 cut(s) 552, 584, 993, 1025
AluBI AGCT 7 cut(s) 73, 278, 360, 447, 669, 765, 1049
AluI AGCT 7 cut(s) 73, 278, 360, 447, 669, 765, 1049
Alw21I GWGCWC 1 cut(s) 892
Alw26I GTCTC 1 cut(s) 547
AlwI GGATC 3 cut(s) 486, 523, 1046
Ama87I CYCGRG 2 cut(s) 39, 912
Aor51HI AGCGCT 1 cut(s) 957
AoxI GGCC 1 cut(s) 915
ApeKI GCWGC 1 cut(s) 962
ApoI RAATTY 4 cut(s) 319, 328, 379, 577
AspLEI GCGC 1 cut(s) 958
AspS9I GGNCC 9 cut(s) 96, 470, 638, 662, 686, 710, 758, 915, 923
AsuHPI GGTGA 3 cut(s) 487, 985, 995
AsuII TTCGAA 1 cut(s) 620
AvaI CYCGRG 2 cut(s) 39, 912
AvaII GGWCC 8 cut(s) 96, 470, 638, 662, 686, 710, 758, 923
BanII GRGCYC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 892
BbvI GCAGC 1 cut(s) 974
BccI CCATC 2 cut(s) 506, 634
BciT130I CCWGG 4 cut(s) 474, 714, 838, 900
BcoDI GTCTC 1 cut(s) 547
BfaI CTAG 3 cut(s) 737, 777, 1032
BfmI CTRYAG 2 cut(s) 232, 266
BfoI RGCGCY 1 cut(s) 959
BfuAI ACCTGC 4 cut(s) 705, 729, 753, 934
BisI GCNGC 1 cut(s) 963
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 1 cut(s) 964
BmcAI AGTACT 1 cut(s) 116
Bme1390I CCNGG 4 cut(s) 474, 714, 838, 900
Bme18I GGWCC 8 cut(s) 96, 470, 638, 662, 686, 710, 758, 923
BmeRI GACNNNNNGTC 1 cut(s) 994
BmeT110I CYCGRG 2 cut(s) 39, 912
BmgT120I GGNCC 9 cut(s) 96, 470, 638, 662, 686, 710, 758, 915, 923
BmiI GGNNCC 1 cut(s) 47
BmrFI CCNGG 4 cut(s) 474, 714, 838, 900
BmsI GCATC 3 cut(s) 121, 143, 1018
BpmI CTGGAG 3 cut(s) 528, 906, 972
Bpu1102I GCTNAGC 1 cut(s) 306
Bpu14I TTCGAA 1 cut(s) 620
BpuEI CTTGAG 1 cut(s) 378
BsaI GGTCTC 1 cut(s) 547
BsaJI CCNNGG 4 cut(s) 177, 473, 498, 836
Bsc4I CCNNNNNNNGG 7 cut(s) 659, 683, 707, 731, 755, 899, 905
Bse118I RCCGGY 1 cut(s) 965
Bse1I ACTGG 2 cut(s) 152, 511
Bse3DI GCAATG 1 cut(s) 942
BseBI CCWGG 4 cut(s) 474, 714, 838, 900
BseDI CCNNGG 4 cut(s) 177, 473, 498, 836
BseLI CCNNNNNNNGG 7 cut(s) 659, 683, 707, 731, 755, 899, 905
BseMI GCAATG 1 cut(s) 942
BseMII CTCAG 3 cut(s) 268, 315, 417
BseNI ACTGG 2 cut(s) 152, 511
BseRI GAGGAG 1 cut(s) 774
BseXI GCAGC 1 cut(s) 974
BsgI GTGCAG 3 cut(s) 103, 203, 504
Bsh1236I CGCG 1 cut(s) 191
BshFI GGCC 1 cut(s) 917
BsiHKAI GWGCWC 1 cut(s) 892
BsiHKCI CYCGRG 2 cut(s) 39, 912
BsiSI CCGG 3 cut(s) 49, 918, 966
BslFI GGGAC 2 cut(s) 46, 286
BslI CCNNNNNNNGG 7 cut(s) 659, 683, 707, 731, 755, 899, 905
BsmAI GTCTC 1 cut(s) 547
BsmFI GGGAC 2 cut(s) 46, 286
BsnI GGCC 1 cut(s) 917
Bso31I GGTCTC 1 cut(s) 547
BsoBI CYCGRG 2 cut(s) 39, 912
Bsp119I TTCGAA 1 cut(s) 620
Bsp1286I GDGCHC 2 cut(s) 48, 892
Bsp143I GATC 5 cut(s) 478, 515, 844, 975, 1051
Bsp1720I GCTNAGC 1 cut(s) 306
BspACI CCGC 6 cut(s) 191, 660, 684, 732, 791, 971
BspANI GGCC 1 cut(s) 917
BspCNI CTCAG 3 cut(s) 267, 316, 418
BspFNI CGCG 1 cut(s) 191
BspLI GGNNCC 1 cut(s) 47
BspMI ACCTGC 4 cut(s) 705, 729, 753, 934
BspPI GGATC 3 cut(s) 486, 523, 1046
BspT104I TTCGAA 1 cut(s) 620
BspTNI GGTCTC 1 cut(s) 547
BsrDI GCAATG 1 cut(s) 942
BsrFI RCCGGY 1 cut(s) 965
BsrI ACTGG 2 cut(s) 152, 511
BssAI RCCGGY 1 cut(s) 965
BssECI CCNNGG 4 cut(s) 177, 473, 498, 836
BssMI GATC 5 cut(s) 478, 515, 844, 975, 1051
BssT1I CCWWGG 2 cut(s) 177, 498
Bst2UI CCWGG 4 cut(s) 474, 714, 838, 900
BstAPI GCANNNNNTGC 1 cut(s) 968
BstBI TTCGAA 1 cut(s) 620
BstC8I GCNNGC 8 cut(s) 658, 678, 682, 702, 726, 730, 750, 960
BstDEI CTNAG 4 cut(s) 254, 306, 324, 426
BstFNI CGCG 1 cut(s) 191
BstH2I RGCGCY 1 cut(s) 959
BstHHI GCGC 1 cut(s) 958
BstKTI GATC 5 cut(s) 481, 518, 847, 978, 1054
BstMAI GTCTC 1 cut(s) 547
BstMBI GATC 5 cut(s) 478, 515, 844, 975, 1051
BstMWI GCNNNNNNNGC 5 cut(s) 666, 690, 738, 962, 968
BstNI CCWGG 4 cut(s) 474, 714, 838, 900
BstSCI CCNGG 4 cut(s) 472, 712, 836, 898
BstSFI CTRYAG 2 cut(s) 232, 266
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 1 cut(s) 974
BstX2I RGATCY 1 cut(s) 515
BstYI RGATCY 1 cut(s) 515
BsuRI GGCC 1 cut(s) 917
BveI ACCTGC 4 cut(s) 705, 729, 753, 934
Cac8I GCNNGC 8 cut(s) 658, 678, 682, 702, 726, 730, 750, 960
CfoI GCGC 1 cut(s) 958
Cfr10I RCCGGY 1 cut(s) 965
Cfr13I GGNCC 9 cut(s) 96, 470, 638, 662, 686, 710, 758, 915, 923
CseI GACGC 1 cut(s) 197
Csp6I GTAC 2 cut(s) 115, 821
CviAII CATG 1 cut(s) 338
CviQI GTAC 2 cut(s) 115, 821
DdeI CTNAG 4 cut(s) 254, 306, 324, 426
DpnI GATC 5 cut(s) 480, 517, 846, 977, 1053
DpnII GATC 5 cut(s) 478, 515, 844, 975, 1051
DriI GACNNNNNGTC 1 cut(s) 994
Eam1105I GACNNNNNGTC 1 cut(s) 994
Eco130I CCWWGG 2 cut(s) 177, 498
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 1 cut(s) 547
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 8 cut(s) 96, 470, 638, 662, 686, 710, 758, 923
Eco47III AGCGCT 1 cut(s) 957
Eco57I CTGAAG 1 cut(s) 999
Eco88I CYCGRG 2 cut(s) 39, 912
EcoO109I RGGNCCY 2 cut(s) 470, 923
EcoRI GAATTC 4 cut(s) 319, 328, 379, 577
EcoRII CCWGG 4 cut(s) 472, 712, 836, 898
EcoRV GATATC 1 cut(s) 33
EcoT14I CCWWGG 2 cut(s) 177, 498
EcoT22I ATGCAT 1 cut(s) 136
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 2 cut(s) 177, 498
FaeI CATG 1 cut(s) 341
FaqI GGGAC 2 cut(s) 46, 286
FatI CATG 1 cut(s) 337
Fnu4HI GCNGC 1 cut(s) 963
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 1 cut(s) 963
FspBI CTAG 3 cut(s) 737, 777, 1032
GlaI GCGC 1 cut(s) 957
GluI GCNGC 1 cut(s) 963
GsuI CTGGAG 3 cut(s) 528, 906, 972
HaeII RGCGCY 1 cut(s) 959
HaeIII GGCC 1 cut(s) 917
HapII CCGG 3 cut(s) 49, 918, 966
HgaI GACGC 1 cut(s) 197
HhaI GCGC 1 cut(s) 958
Hin1II CATG 1 cut(s) 341
Hin6I GCGC 1 cut(s) 956
HinP1I GCGC 1 cut(s) 956
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 2 cut(s) 194, 607
HpaII CCGG 3 cut(s) 49, 918, 966
HphI GGTGA 3 cut(s) 487, 985, 995
Hpy166II GTNNAC 2 cut(s) 96, 758
Hpy188I TCNGA 3 cut(s) 257, 325, 427
Hpy188III TCNNGA 5 cut(s) 138, 289, 777, 885, 999
Hpy8I GTNNAC 2 cut(s) 96, 758
Hpy99I CGWCG 1 cut(s) 61
HpyAV CCTTC 4 cut(s) 431, 612, 783, 1023
HpyCH4V TGCA 7 cut(s) 120, 134, 146, 220, 521, 590, 935
HpyF10VI GCNNNNNNNGC 5 cut(s) 666, 690, 738, 962, 968
HpyF3I CTNAG 4 cut(s) 254, 306, 324, 426
Hsp92II CATG 1 cut(s) 341
HspAI GCGC 1 cut(s) 956
Kzo9I GATC 5 cut(s) 478, 515, 844, 975, 1051
LmnI GCTCC 4 cut(s) 51, 357, 887, 953
Lsp1109I GCAGC 1 cut(s) 974
LweI GCATC 3 cut(s) 121, 143, 1018
MaeI CTAG 3 cut(s) 737, 777, 1032
MaeIII GTNAC 2 cut(s) 644, 1036
MalI GATC 5 cut(s) 480, 517, 846, 977, 1053
MboI GATC 5 cut(s) 478, 515, 844, 975, 1051
MboII GAAGA 2 cut(s) 359, 970
MfeI CAATTG 1 cut(s) 365
MflI RGATCY 1 cut(s) 515
MhlI GDGCHC 2 cut(s) 48, 892
MluCI AATT 6 cut(s) 319, 328, 365, 379, 566, 577
MlyI GAGTC 1 cut(s) 601
MmeI TCCRAC 3 cut(s) 408, 616, 793
Mph1103I ATGCAT 1 cut(s) 136
MspA1I CMGCKG 1 cut(s) 1049
MspI CCGG 3 cut(s) 49, 918, 966
MspR9I CCNGG 4 cut(s) 474, 714, 838, 900
MunI CAATTG 1 cut(s) 365
MvaI CCWGG 4 cut(s) 474, 714, 838, 900
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 5 cut(s) 666, 690, 738, 962, 968
NdeII GATC 5 cut(s) 478, 515, 844, 975, 1051
NlaIII CATG 1 cut(s) 341
NlaIV GGNNCC 1 cut(s) 47
NsiI ATGCAT 1 cut(s) 136
NspV TTCGAA 1 cut(s) 620
PaeR7I CTCGAG 1 cut(s) 39
PfeI GAWTC 1 cut(s) 194
PflMI CCANNNNNTGG 2 cut(s) 707, 755
PkrI GCNGC 1 cut(s) 964
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
PpuMI RGGWCCY 2 cut(s) 470, 923
Psp5II RGGWCCY 2 cut(s) 470, 923
Psp6I CCWGG 4 cut(s) 472, 712, 836, 898
PspGI CCWGG 4 cut(s) 472, 712, 836, 898
PspN4I GGNNCC 1 cut(s) 47
PspPI GGNCC 9 cut(s) 96, 470, 638, 662, 686, 710, 758, 915, 923
PspPPI RGGWCCY 2 cut(s) 470, 923
PspXI VCTCGAGB 1 cut(s) 39
PsuI RGATCY 1 cut(s) 515
PvuII CAGCTG 1 cut(s) 1049
RsaI GTAC 2 cut(s) 116, 822
RsaNI GTAC 2 cut(s) 115, 821
SatI GCNGC 1 cut(s) 963
Sau3AI GATC 5 cut(s) 478, 515, 844, 975, 1051
Sau96I GGNCC 9 cut(s) 96, 470, 638, 662, 686, 710, 758, 915, 923
ScaI AGTACT 1 cut(s) 116
SchI GAGTC 1 cut(s) 601
ScrFI CCNGG 4 cut(s) 474, 714, 838, 900
SduI GDGCHC 2 cut(s) 48, 892
SfaNI GCATC 3 cut(s) 121, 143, 1018
SfcI CTRYAG 2 cut(s) 232, 266
Sfr274I CTCGAG 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 620
SinI GGWCC 8 cut(s) 96, 470, 638, 662, 686, 710, 758, 923
SlaI CTCGAG 1 cut(s) 39
SmlI CTYRAG 2 cut(s) 39, 393
SmoI CTYRAG 2 cut(s) 39, 393
Sse9I AATT 6 cut(s) 319, 328, 365, 379, 566, 577
SsiI CCGC 6 cut(s) 191, 660, 684, 732, 791, 971
SspMI CTAG 3 cut(s) 737, 777, 1032
StyD4I CCNGG 4 cut(s) 472, 712, 836, 898
StyI CCWWGG 2 cut(s) 177, 498
TaqI TCGA 4 cut(s) 40, 605, 620, 847
TasI AATT 6 cut(s) 319, 328, 365, 379, 566, 577
TatI WGTACW 1 cut(s) 114
TfiI GAWTC 1 cut(s) 194
TseI GCWGC 1 cut(s) 962
TspDTI ATGAA 1 cut(s) 590
Van91I CCANNNNNTGG 2 cut(s) 707, 755
VpaK11BI GGWCC 8 cut(s) 96, 470, 638, 662, 686, 710, 758, 923
XapI RAATTY 4 cut(s) 319, 328, 379, 577
XbaI TCTAGA 1 cut(s) 776
XhoI CTCGAG 1 cut(s) 39
XspI CTAG 3 cut(s) 737, 777, 1032
ZrmI AGTACT 1 cut(s) 116
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.