MD07G1238500.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014972
Physical Location & Seq
Reverse (-)
31037643 .. 31038368
726 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1238500.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGGGAAACTCTGGGTTGAAGTGTGCCTGATATCCGCTCGAGGGCTCACGCGTTCCTCTTCGTTATGGAAGCTCCAGTGGTATGCTGTTGGGTGGACTAATCCCAACAACAAATACTGCACCAAGATTGATGCATCTGGAAATGCAAATCCGGTTTGGAGAACCAAGTTTGCTACCTTGGTTGAGGGCTCGGAGGCGGACTTGCAGGATCTGGCGCTGCACATTGATGTGTACAGCAGAGAGCCTATATTCCTTAGGGAAAAGCTTCAGGGGACGGCAACTATTGTCCTGAGAGAGTTTCTGGCTAAGCATAACAAGAACTCTGGGGCTCCAAGGCAAGGAGCTGAAGAAGTCGGGAGCTACCAGTTGCGGAAGAAGAACTCCAACAAACCTCAAGGGTTCATCGATGTTTCAATACGAATTTCCGAGGATATGGAAGCTCGAAGCTCGTACACAGTGAGGGAGGACCTATGGATCACAGCAACACCATCACCTTGGCTACTGGAGACGGGTCTGCACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

175

Amino Acids

19.71

Weight (kDa)

8.88

Isoelectric Point (pI)

50.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 105 1.3e-07 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40815 AT2G40815
fragaria_vesca FvH4_7g26311
malus_domestica MD01G1172000.v1.1 MD07G1238500.v1.1
prunus_persica Prupe.2G267300_v2.0.a1
pyrus_communis pycom07g21450
rosa_chinensis RchiOBHm_Chr1g0372771
rosa_laevigata RLG00000026873
rosa_multiflora Rmu_sc0029212.1_g000001
rosa_rugosa Rorug01G0042900 Rorug01G0374300
rosa_samantha Rh1AG383000 Rh1CG359900 Rh1DG377600
rosa_wichuraiana Rw1G033710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 38
AccII CGCG 1 cut(s) 52
AciI CCGC 3 cut(s) 36, 197, 370
AclWI GGATC 2 cut(s) 216, 482
AcsI RAATTY 1 cut(s) 420
AcuI CTGAAG 2 cut(s) 251, 366
AfaI GTAC 2 cut(s) 233, 452
AfiI CCNNNNNNNGG 2 cut(s) 42, 338
AflIII ACRYGT 1 cut(s) 50
AgsI TTSAA 2 cut(s) 20, 414
AluBI AGCT 6 cut(s) 73, 265, 344, 360, 440, 447
AluI AGCT 6 cut(s) 73, 265, 344, 360, 440, 447
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 2 cut(s) 216, 482
AlwNI CAGNNNCTG 1 cut(s) 211
Ama87I CYCGRG 1 cut(s) 39
ApeKI GCWGC 1 cut(s) 217
ApoI RAATTY 1 cut(s) 420
Asp700I GAANNNNTTC 1 cut(s) 264
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 1 cut(s) 466
AsuHPI GGTGA 1 cut(s) 483
AvaI CYCGRG 1 cut(s) 39
AvaII GGWCC 1 cut(s) 466
AxyI CCTNAGG 1 cut(s) 254
BanII GRGCYC 3 cut(s) 48, 191, 331
BbvI GCAGC 1 cut(s) 204
BccI CCATC 1 cut(s) 496
BceAI ACGGC 1 cut(s) 291
BcoDI GTCTC 1 cut(s) 500
BfoI RGCGCY 1 cut(s) 218
BisI GCNGC 1 cut(s) 218
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 1 cut(s) 219
Bme18I GGWCC 1 cut(s) 466
BmeT110I CYCGRG 1 cut(s) 39
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 1 cut(s) 330
BmsI GCATC 2 cut(s) 121, 143
BpmI CTGGAG 2 cut(s) 59, 524
Bpu1102I GCTNAGC 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 378
Bsa29I ATCGAT 1 cut(s) 405
BsaJI CCNNGG 4 cut(s) 177, 332, 426, 494
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 2 cut(s) 42, 338
Bse1I ACTGG 4 cut(s) 76, 364, 507, 520
Bse21I CCTNAGG 1 cut(s) 254
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 4 cut(s) 177, 332, 426, 494
BseLI CCNNNNNNNGG 2 cut(s) 42, 338
BseMII CTCAG 1 cut(s) 281
BseNI ACTGG 4 cut(s) 76, 364, 507, 520
BseXI GCAGC 1 cut(s) 204
BsgI GTGCAG 3 cut(s) 103, 203, 500
Bsh1236I CGCG 1 cut(s) 52
BshVI ATCGAT 1 cut(s) 405
BsiHKCI CYCGRG 1 cut(s) 39
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 2 cut(s) 42, 338
BsmAI GTCTC 1 cut(s) 500
BsmBI CGTCTC 1 cut(s) 500
BsmFI GGGAC 1 cut(s) 286
BsoBI CYCGRG 1 cut(s) 39
Bsp1286I GDGCHC 3 cut(s) 48, 191, 331
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 2 cut(s) 208, 474
Bsp1720I GCTNAGC 1 cut(s) 306
BspACI CCGC 3 cut(s) 36, 197, 370
BspCNI CTCAG 1 cut(s) 282
BspDI ATCGAT 1 cut(s) 405
BspFNI CGCG 1 cut(s) 52
BspLI GGNNCC 1 cut(s) 330
BspPI GGATC 2 cut(s) 216, 482
BsrBI CCGCTC 1 cut(s) 38
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 4 cut(s) 76, 364, 507, 520
BssECI CCNNGG 4 cut(s) 177, 332, 426, 494
BssMI GATC 2 cut(s) 208, 474
BssT1I CCWWGG 3 cut(s) 177, 332, 494
Bst4CI ACNGT 1 cut(s) 457
Bst6I CTCTTC 1 cut(s) 64
BstAUI TGTACA 1 cut(s) 231
BstDEI CTNAG 3 cut(s) 254, 290, 306
BstFNI CGCG 1 cut(s) 52
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 2 cut(s) 211, 477
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 2 cut(s) 208, 474
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 208
BstXI CCANNNNNNTGG 1 cut(s) 495
BstYI RGATCY 1 cut(s) 208
Bsu15I ATCGAT 1 cut(s) 405
Bsu36I CCTNAGG 1 cut(s) 254
BsuTUI ATCGAT 1 cut(s) 405
BtsIMutI CAGTG 2 cut(s) 83, 462
CaiI CAGNNNCTG 1 cut(s) 211
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 1 cut(s) 466
ClaI ATCGAT 1 cut(s) 405
Csp6I GTAC 2 cut(s) 232, 451
CviQI GTAC 2 cut(s) 232, 451
DdeI CTNAG 3 cut(s) 254, 290, 306
DpnI GATC 2 cut(s) 210, 476
DpnII GATC 2 cut(s) 208, 474
Eam1104I CTCTTC 1 cut(s) 64
EarI CTCTTC 1 cut(s) 64
EciI GGCGGA 1 cut(s) 212
Eco130I CCWWGG 3 cut(s) 177, 332, 494
Eco24I GRGCYC 3 cut(s) 48, 191, 331
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 466
Eco57I CTGAAG 2 cut(s) 251, 366
Eco81I CCTNAGG 1 cut(s) 254
Eco88I CYCGRG 1 cut(s) 39
EcoO109I RGGNCCY 1 cut(s) 466
EcoRV GATATC 1 cut(s) 33
EcoT14I CCWWGG 3 cut(s) 177, 332, 494
EcoT22I ATGCAT 1 cut(s) 136
EcoT38I GRGCYC 3 cut(s) 48, 191, 331
ErhI CCWWGG 3 cut(s) 177, 332, 494
Esp3I CGTCTC 1 cut(s) 500
FaiI YATR 6 cut(s) 67, 84, 248, 312, 434, 472
FaqI GGGAC 1 cut(s) 286
Fnu4HI GCNGC 1 cut(s) 218
FriOI GRGCYC 3 cut(s) 48, 191, 331
Fsp4HI GCNGC 1 cut(s) 218
GlaI GCGC 1 cut(s) 216
GluI GCNGC 1 cut(s) 218
GsuI CTGGAG 2 cut(s) 59, 524
HaeII RGCGCY 1 cut(s) 218
HapII CCGG 1 cut(s) 152
HhaI GCGC 1 cut(s) 217
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HindIII AAGCTT 1 cut(s) 263
HpaII CCGG 1 cut(s) 152
HphI GGTGA 1 cut(s) 483
Hpy166II GTNNAC 3 cut(s) 96, 232, 453
Hpy188I TCNGA 2 cut(s) 193, 427
Hpy188III TCNNGA 3 cut(s) 138, 289, 355
Hpy8I GTNNAC 3 cut(s) 96, 232, 453
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4V TGCA 6 cut(s) 120, 134, 146, 205, 220, 517
HpyF3I CTNAG 3 cut(s) 254, 290, 306
HspAI GCGC 1 cut(s) 215
Kzo9I GATC 2 cut(s) 208, 474
LmnI GCTCC 4 cut(s) 78, 334, 341, 357
Lsp1109I GCAGC 1 cut(s) 204
LweI GCATC 2 cut(s) 121, 143
MalI GATC 2 cut(s) 210, 476
MbiI CCGCTC 1 cut(s) 38
MboI GATC 2 cut(s) 208, 474
MboII GAAGA 4 cut(s) 51, 359, 385, 388
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 3 cut(s) 48, 191, 331
MluCI AATT 1 cut(s) 420
MluI ACGCGT 1 cut(s) 50
MmeI TCCRAC 1 cut(s) 408
MnlI CCTC 8 cut(s) 35, 67, 178, 187, 402, 421, 453, 457
Mph1103I ATGCAT 1 cut(s) 136
MroXI GAANNNNTTC 1 cut(s) 264
MslI CAYNNNNRTG 2 cut(s) 225, 227
MspI CCGG 1 cut(s) 152
MvnI CGCG 1 cut(s) 52
NdeII GATC 2 cut(s) 208, 474
NlaIV GGNNCC 1 cut(s) 330
NsiI ATGCAT 1 cut(s) 136
PaeR7I CTCGAG 1 cut(s) 39
PdmI GAANNNNTTC 1 cut(s) 264
PkrI GCNGC 1 cut(s) 219
PpuMI RGGWCCY 1 cut(s) 466
Psp5II RGGWCCY 1 cut(s) 466
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 1 cut(s) 466
PspPPI RGGWCCY 1 cut(s) 466
PspXI VCTCGAGB 1 cut(s) 39
PstNI CAGNNNCTG 1 cut(s) 211
PsuI RGATCY 1 cut(s) 208
RsaI GTAC 2 cut(s) 233, 452
RsaNI GTAC 2 cut(s) 232, 451
RseI CAYNNNNRTG 2 cut(s) 225, 227
SatI GCNGC 1 cut(s) 218
Sau3AI GATC 2 cut(s) 208, 474
Sau96I GGNCC 1 cut(s) 466
SduI GDGCHC 3 cut(s) 48, 191, 331
SfaNI GCATC 2 cut(s) 121, 143
Sfr274I CTCGAG 1 cut(s) 39
SinI GGWCC 1 cut(s) 466
SlaI CTCGAG 1 cut(s) 39
SmiMI CAYNNNNRTG 2 cut(s) 225, 227
SmlI CTYRAG 2 cut(s) 39, 393
SmoI CTYRAG 2 cut(s) 39, 393
Sse9I AATT 1 cut(s) 420
SsiI CCGC 3 cut(s) 36, 197, 370
StyI CCWWGG 3 cut(s) 177, 332, 494
TaaI ACNGT 1 cut(s) 457
TaqI TCGA 3 cut(s) 40, 405, 442
TasI AATT 1 cut(s) 420
TatI WGTACW 1 cut(s) 231
TscAI CASTG 2 cut(s) 83, 462
TseI GCWGC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 391
TspRI CASTG 2 cut(s) 83, 462
VpaK11BI GGWCC 1 cut(s) 466
XapI RAATTY 1 cut(s) 420
XhoI CTCGAG 1 cut(s) 39
XmnI GAANNNNTTC 1 cut(s) 264
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.