Prupe.2G267300_v2.0.a1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0014972
Physical Location & Seq
Reverse (-)
27325404 .. 27326678
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G267300.1

Sequence Viewer

Length: 1002 bp
ATGGGGAAACTCTGGGTTGAAGTGTGCCTGATATCTGCTAGGGGGCTCCGGCGTTCGTCTTCCTTATGGAAGCTCCAATGGTATGCCGTTGGGTGGACTAACCCGAATAACAAATACTGCACCAAGATTGATGCATCTGGAAATGCAAATCCAGTATGGAAAACCAAGTTTGCTACCTTGGTTGAGGACTCAGAGTCGAACTTGAAGGATCTGGCGCTGCACATTGAAGTGTACAGCAGAGAGCCTATATTCCTCAGAGAGAGGCTTCAGGGTACAGCAACTATTGTCTTGAGAGAGTTTCTGGCTAAGCATAACAAGAATTCTGAGGCTTCAAGACAAGGAGCTGAAGAAGTTGGGAGCTATCAATTGCGGAAAAAGAACTCCAACAAGCCTCAAGGATTCGTAGATGTTTCAATACGTATATCTGAGGACATGGAAGCTCGAAGCTCATACACAGGCAGTGAGGGAGGACCTACGGATCTCAGCAATACCATCACCTTGGCTATTGGAGATGGGTCTGCACCAACATTTCAACCTCTAGCGCCACACCAAAGGCCAGAAAGTCAGCTCCGTATCAATTCTCCATATGCACATCCAAGGCCTTCCCCTACAAACTATTCCAACCCATATGCAGTTGGACCAAGCCACCCACCATCAGGCGGACCAAGTTACCGGCCATCCAGCGGACCAAGCTACCCGCCAGCAAGTGGACCAAGCTATGAGCCGCCCAAAACTCCGCCACCACCTCCCCCACCTTCTAATGTTGGGTACATACCCACTTTTATCCCAAGAACAGATAGAATGTCGGATACCTATGTTAATATGCCATCATCTGGAGCACCGCCTGGTCGTAGAGGGGCACCGGGTTTTGGAATGGGAATGGGTGCTGGAGCGCTGGCGGCCGGTGCTGTGATCTTCGGTGATGACTTTATGTCAGGATTTGATGTGCCTTCAGGATTACAAGATGCTAGTCTCACCATATCAACTGATCCTCCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

334

Amino Acids

35.85

Weight (kDa)

8.73

Isoelectric Point (pI)

63.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40815 AT2G40815
fragaria_vesca FvH4_7g26311
malus_domestica MD01G1172000.v1.1 MD07G1238500.v1.1
prunus_persica Prupe.2G267300_v2.0.a1
pyrus_communis pycom07g21450
rosa_chinensis RchiOBHm_Chr1g0372771
rosa_laevigata RLG00000026873
rosa_multiflora Rmu_sc0029212.1_g000001
rosa_rugosa Rorug01G0042900 Rorug01G0374300
rosa_samantha Rh1AG383000 Rh1CG359900 Rh1DG377600
rosa_wichuraiana Rw1G033710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 859
AccB7I CCANNNNNTGG 2 cut(s) 707, 833
AciI CCGC 8 cut(s) 370, 660, 684, 698, 725, 737, 842, 899
AclWI GGATC 3 cut(s) 216, 486, 983
AcoI YGGCCR 2 cut(s) 674, 900
AcsI RAATTY 1 cut(s) 319
AcuI CTGAAG 3 cut(s) 251, 366, 936
AfaI GTAC 3 cut(s) 233, 274, 770
AfeI AGCGCT 1 cut(s) 894
AfiI CCNNNNNNNGG 7 cut(s) 93, 656, 659, 683, 707, 833, 869
AgsI TTSAA 6 cut(s) 20, 205, 227, 333, 414, 533
AhdI GACNNNNNGTC 2 cut(s) 193, 931
AjnI CCWGG 1 cut(s) 844
AluBI AGCT 8 cut(s) 73, 344, 360, 440, 447, 568, 693, 717
AluI AGCT 8 cut(s) 73, 344, 360, 440, 447, 568, 693, 717
Alw21I GWGCWC 1 cut(s) 841
Alw26I GTCTC 1 cut(s) 977
AlwI GGATC 3 cut(s) 216, 486, 983
Aor51HI AGCGCT 1 cut(s) 894
AoxI GGCC 4 cut(s) 554, 599, 674, 900
ApeKI GCWGC 1 cut(s) 217
ApoI RAATTY 1 cut(s) 319
AspLEI GCGC 3 cut(s) 217, 544, 895
AspS9I GGNCC 5 cut(s) 470, 638, 662, 686, 710
AsuC2I CCSGG 1 cut(s) 864
AsuHPI GGTGA 3 cut(s) 487, 932, 967
AvaII GGWCC 5 cut(s) 470, 638, 662, 686, 710
BaeGI GKGCMC 1 cut(s) 862
BaeI ACNNNNGTAYC 2 cut(s) 801, 834
BanI GGYRCC 1 cut(s) 859
BanII GRGCYC 1 cut(s) 48
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 841
BbvI GCAGC 1 cut(s) 204
BccI CCATC 5 cut(s) 500, 506, 661, 685, 835
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 1 cut(s) 846
BciVI GTATCC 1 cut(s) 802
BcnI CCSGG 1 cut(s) 864
BcoDI GTCTC 1 cut(s) 977
BfaI CTAG 3 cut(s) 39, 539, 969
BfoI RGCGCY 3 cut(s) 218, 545, 896
BfuI GTATCC 1 cut(s) 802
BisI GCNGC 3 cut(s) 218, 725, 900
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 3 cut(s) 219, 726, 901
Bme1390I CCNGG 2 cut(s) 846, 864
Bme18I GGWCC 5 cut(s) 470, 638, 662, 686, 710
BmeRI GACNNNNNGTC 2 cut(s) 193, 931
BmgT120I GGNCC 5 cut(s) 470, 638, 662, 686, 710
BmiI GGNNCC 2 cut(s) 47, 861
BmrFI CCNGG 2 cut(s) 846, 864
BmsI GCATC 3 cut(s) 121, 143, 955
BpiI GAAGAC 1 cut(s) 51
BpmI CTGGAG 2 cut(s) 855, 909
Bpu1102I GCTNAGC 1 cut(s) 306
BpuEI CTTGAG 2 cut(s) 310, 378
BpuMI CCSGG 1 cut(s) 864
BsaAI YACGTR 1 cut(s) 419
BsaJI CCNNGG 3 cut(s) 177, 498, 596
BsaXI ACNNNNNCTCC 1 cut(s) 976
Bsc4I CCNNNNNNNGG 7 cut(s) 93, 656, 659, 683, 707, 833, 869
Bse118I RCCGGY 2 cut(s) 672, 902
Bse1I ACTGG 1 cut(s) 152
BseBI CCWGG 1 cut(s) 846
BseDI CCNNGG 3 cut(s) 177, 498, 596
BseGI GGATG 2 cut(s) 592, 677
BseLI CCNNNNNNNGG 7 cut(s) 93, 656, 659, 683, 707, 833, 869
BseMII CTCAG 5 cut(s) 204, 268, 315, 417, 496
BseNI ACTGG 1 cut(s) 152
BseSI GKGCMC 1 cut(s) 862
BseX3I CGGCCG 1 cut(s) 900
BseXI GCAGC 1 cut(s) 204
BsgI GTGCAG 3 cut(s) 103, 203, 504
Bsh1285I CGRYCG 1 cut(s) 903
BshFI GGCC 4 cut(s) 556, 601, 676, 902
BshNI GGYRCC 1 cut(s) 859
BsiEI CGRYCG 1 cut(s) 903
BsiHKAI GWGCWC 1 cut(s) 841
BsiSI CCGG 4 cut(s) 49, 673, 863, 903
BslI CCNNNNNNNGG 7 cut(s) 93, 656, 659, 683, 707, 833, 869
BsmAI GTCTC 1 cut(s) 977
BsnI GGCC 4 cut(s) 556, 601, 676, 902
Bsp1286I GDGCHC 3 cut(s) 48, 841, 862
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 4 cut(s) 208, 478, 912, 988
Bsp1720I GCTNAGC 1 cut(s) 306
BspACI CCGC 8 cut(s) 370, 660, 684, 698, 725, 737, 842, 899
BspANI GGCC 4 cut(s) 556, 601, 676, 902
BspCNI CTCAG 5 cut(s) 203, 267, 316, 418, 495
BspLI GGNNCC 2 cut(s) 47, 861
BspPI GGATC 3 cut(s) 216, 486, 983
BspT107I GGYRCC 1 cut(s) 859
BsrFI RCCGGY 2 cut(s) 672, 902
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 1 cut(s) 152
BssAI RCCGGY 2 cut(s) 672, 902
BssECI CCNNGG 3 cut(s) 177, 498, 596
BssMI GATC 4 cut(s) 208, 478, 912, 988
BssT1I CCWWGG 3 cut(s) 177, 498, 596
Bst2UI CCWGG 1 cut(s) 846
BstAUI TGTACA 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 419
BstC8I GCNNGC 2 cut(s) 702, 897
BstDEI CTNAG 6 cut(s) 190, 254, 306, 324, 426, 482
BstF5I GGATG 2 cut(s) 592, 677
BstH2I RGCGCY 3 cut(s) 218, 545, 896
BstHHI GCGC 3 cut(s) 217, 544, 895
BstKTI GATC 4 cut(s) 211, 481, 915, 991
BstMAI GTCTC 1 cut(s) 977
BstMBI GATC 4 cut(s) 208, 478, 912, 988
BstMCI CGRYCG 1 cut(s) 903
BstMWI GCNNNNNNNGC 3 cut(s) 690, 899, 905
BstNI CCWGG 1 cut(s) 846
BstSCI CCNGG 2 cut(s) 844, 862
BstSLI GKGCMC 1 cut(s) 862
BstSNI TACGTA 1 cut(s) 419
BstV1I GCAGC 1 cut(s) 204
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 2 cut(s) 208, 478
BstXI CCANNNNNNTGG 1 cut(s) 499
BstYI RGATCY 2 cut(s) 208, 478
BstZI CGGCCG 1 cut(s) 900
BsuI GTATCC 1 cut(s) 802
BsuRI GGCC 4 cut(s) 556, 601, 676, 902
BtsCI GGATG 2 cut(s) 592, 677
BtsI GCAGTG 1 cut(s) 466
BtsIMutI CAGTG 1 cut(s) 466
Cac8I GCNNGC 2 cut(s) 702, 897
CfoI GCGC 3 cut(s) 217, 544, 895
Cfr10I RCCGGY 2 cut(s) 672, 902
Cfr13I GGNCC 5 cut(s) 470, 638, 662, 686, 710
Csp6I GTAC 3 cut(s) 232, 273, 769
CviAII CATG 1 cut(s) 433
CviQI GTAC 3 cut(s) 232, 273, 769
DdeI CTNAG 6 cut(s) 190, 254, 306, 324, 426, 482
DpnI GATC 4 cut(s) 210, 480, 914, 990
DpnII GATC 4 cut(s) 208, 478, 912, 988
DriI GACNNNNNGTC 2 cut(s) 193, 931
EaeI YGGCCR 2 cut(s) 674, 900
EagI CGGCCG 1 cut(s) 900
Eam1105I GACNNNNNGTC 2 cut(s) 193, 931
EciI GGCGGA 2 cut(s) 675, 726
EclXI CGGCCG 1 cut(s) 900
Eco105I TACGTA 1 cut(s) 419
Eco130I CCWWGG 3 cut(s) 177, 498, 596
Eco147I AGGCCT 1 cut(s) 601
Eco24I GRGCYC 1 cut(s) 48
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 5 cut(s) 470, 638, 662, 686, 710
Eco47III AGCGCT 1 cut(s) 894
Eco52I CGGCCG 1 cut(s) 900
Eco57I CTGAAG 3 cut(s) 251, 366, 936
EcoO109I RGGNCCY 1 cut(s) 470
EcoRI GAATTC 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 844
EcoRV GATATC 1 cut(s) 33
EcoT14I CCWWGG 3 cut(s) 177, 498, 596
EcoT22I ATGCAT 1 cut(s) 136
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 3 cut(s) 177, 498, 596
FaeI CATG 1 cut(s) 436
FatI CATG 1 cut(s) 432
FauI CCCGC 1 cut(s) 705
FauNDI CATATG 2 cut(s) 586, 628
Fnu4HI GCNGC 3 cut(s) 218, 725, 900
FokI GGATG 2 cut(s) 579, 664
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 3 cut(s) 218, 725, 900
FspBI CTAG 3 cut(s) 39, 539, 969
GlaI GCGC 3 cut(s) 216, 543, 894
GluI GCNGC 3 cut(s) 218, 725, 900
GsuI CTGGAG 2 cut(s) 855, 909
HaeII RGCGCY 3 cut(s) 218, 545, 896
HaeIII GGCC 4 cut(s) 556, 601, 676, 902
HapII CCGG 4 cut(s) 49, 673, 863, 903
HhaI GCGC 3 cut(s) 217, 544, 895
Hin1II CATG 1 cut(s) 436
Hin6I GCGC 3 cut(s) 215, 542, 893
HinP1I GCGC 3 cut(s) 215, 542, 893
HinfI GANTC 3 cut(s) 188, 194, 399
HpaII CCGG 4 cut(s) 49, 673, 863, 903
HphI GGTGA 3 cut(s) 487, 932, 967
Hpy166II GTNNAC 3 cut(s) 96, 232, 710
Hpy188I TCNGA 5 cut(s) 193, 257, 325, 427, 808
Hpy188III TCNNGA 6 cut(s) 138, 289, 333, 834, 936, 954
Hpy8I GTNNAC 3 cut(s) 96, 232, 710
HpyAV CCTTC 4 cut(s) 199, 612, 765, 960
HpyCH4IV ACGT 1 cut(s) 418
HpyCH4V TGCA 7 cut(s) 120, 134, 146, 220, 521, 590, 632
HpyF10VI GCNNNNNNNGC 3 cut(s) 690, 899, 905
HpyF3I CTNAG 6 cut(s) 190, 254, 306, 324, 426, 482
HpySE526I ACGT 1 cut(s) 418
Hsp92II CATG 1 cut(s) 436
HspAI GCGC 3 cut(s) 215, 542, 893
Kzo9I GATC 4 cut(s) 208, 478, 912, 988
LmnI GCTCC 7 cut(s) 51, 78, 341, 357, 573, 836, 890
Lsp1109I GCAGC 1 cut(s) 204
LweI GCATC 3 cut(s) 121, 143, 955
MaeI CTAG 3 cut(s) 39, 539, 969
MaeII ACGT 1 cut(s) 418
MaeIII GTNAC 1 cut(s) 668
MalI GATC 4 cut(s) 210, 480, 914, 990
MboI GATC 4 cut(s) 208, 478, 912, 988
MboII GAAGA 3 cut(s) 51, 359, 907
MfeI CAATTG 1 cut(s) 365
MflI RGATCY 2 cut(s) 208, 478
MhlI GDGCHC 3 cut(s) 48, 841, 862
MluCI AATT 3 cut(s) 319, 365, 577
MlyI GAGTC 2 cut(s) 182, 203
MmeI TCCRAC 4 cut(s) 408, 616, 645, 786
Mph1103I ATGCAT 1 cut(s) 136
MseI TTAA 2 cut(s) 819, 1000
MslI CAYNNNNRTG 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 684
MspI CCGG 4 cut(s) 49, 673, 863, 903
MspR9I CCNGG 2 cut(s) 846, 864
MunI CAATTG 1 cut(s) 365
MvaI CCWGG 1 cut(s) 846
MwoI GCNNNNNNNGC 3 cut(s) 690, 899, 905
NciI CCSGG 1 cut(s) 864
NdeI CATATG 2 cut(s) 586, 628
NdeII GATC 4 cut(s) 208, 478, 912, 988
NlaIII CATG 1 cut(s) 436
NlaIV GGNNCC 2 cut(s) 47, 861
NsiI ATGCAT 1 cut(s) 136
PceI AGGCCT 1 cut(s) 601
PfeI GAWTC 1 cut(s) 399
PflMI CCANNNNNTGG 2 cut(s) 707, 833
PkrI GCNGC 3 cut(s) 219, 726, 901
PleI GAGTC 2 cut(s) 182, 202
PpsI GAGTC 2 cut(s) 182, 202
Ppu21I YACGTR 1 cut(s) 419
PpuMI RGGWCCY 1 cut(s) 470
Psp5II RGGWCCY 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 844
PspGI CCWGG 1 cut(s) 844
PspN4I GGNNCC 2 cut(s) 47, 861
PspPI GGNCC 5 cut(s) 470, 638, 662, 686, 710
PspPPI RGGWCCY 1 cut(s) 470
PsuI RGATCY 2 cut(s) 208, 478
RsaI GTAC 3 cut(s) 233, 274, 770
RsaNI GTAC 3 cut(s) 232, 273, 769
RseI CAYNNNNRTG 1 cut(s) 227
SaqAI TTAA 2 cut(s) 819, 1000
SatI GCNGC 3 cut(s) 218, 725, 900
Sau3AI GATC 4 cut(s) 208, 478, 912, 988
Sau96I GGNCC 5 cut(s) 470, 638, 662, 686, 710
SchI GAGTC 2 cut(s) 182, 203
ScrFI CCNGG 2 cut(s) 846, 864
SduI GDGCHC 3 cut(s) 48, 841, 862
SfaNI GCATC 3 cut(s) 121, 143, 955
SinI GGWCC 5 cut(s) 470, 638, 662, 686, 710
SmiMI CAYNNNNRTG 1 cut(s) 227
SmlI CTYRAG 2 cut(s) 289, 393
SmoI CTYRAG 2 cut(s) 289, 393
SnaBI TACGTA 1 cut(s) 419
Sse9I AATT 3 cut(s) 319, 365, 577
SseBI AGGCCT 1 cut(s) 601
SsiI CCGC 8 cut(s) 370, 660, 684, 698, 725, 737, 842, 899
SspMI CTAG 3 cut(s) 39, 539, 969
StuI AGGCCT 1 cut(s) 601
StyD4I CCNGG 2 cut(s) 844, 862
StyI CCWWGG 3 cut(s) 177, 498, 596
TaiI ACGT 1 cut(s) 421
TaqI TCGA 2 cut(s) 197, 442
TasI AATT 3 cut(s) 319, 365, 577
TatI WGTACW 1 cut(s) 231
TauI GCSGC 2 cut(s) 727, 902
TfiI GAWTC 1 cut(s) 399
Tru1I TTAA 2 cut(s) 819, 1000
Tru9I TTAA 2 cut(s) 819, 1000
TscAI CASTG 1 cut(s) 466
TseI GCWGC 1 cut(s) 217
TspGWI ACGGA 2 cut(s) 491, 560
TspRI CASTG 1 cut(s) 466
Van91I CCANNNNNTGG 2 cut(s) 707, 833
VpaK11BI GGWCC 5 cut(s) 470, 638, 662, 686, 710
XapI RAATTY 1 cut(s) 319
XspI CTAG 3 cut(s) 39, 539, 969
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.