MD01G1172000.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014972
Physical Location & Seq
Reverse (-)
27433116 .. 27434505
1390 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1172000.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGGGGAAGCTCTGGGTTGAAGTGTGCTTGATATCCGCCCGAGGGCTCAGGCGTTCGTCTTCGTTGTGGAAGCTCCAGTGGTATGCTGTTGGGTGGACTAATCCGAATAACAAGTACTGCACCAAGATTGATGCTTCTGGAAATGAGAATCCGGTGTGGAGAACCAAGTTTGCTACCTTGATTGAGGGCTCGGAGGCGGACTTGCAGGATCTGGCGCTGCACGTTGAGGTGTACAGCAGAGAGCCTATCTTCCTCCGGGAAAAGCTTCAGGGGACGGCAACTATTGTCCTGAGAGAGTTTCTGGCTAAGCATAACAAGAACTCTGAGGCTCCGAGGCATGGAGCTGAAGAAGTTGGGAGCTACCAGTTGCGGAAAAAGAATTCCAACAAACCTCAAGGGTTCGTCGATGTCTCAATACGTATTTCTGAGGATATGGAAGCTCGAAGCTCGTACACAGGCAGTGAAGGAGGACCTATGGATAACAGCATTACTATCACCTTGGCTACCGGAGACGGATCTGCACCAGCGATACGACCTGTAGCTCGACAACAAAGGCCGGAAGATCAATTCCGTATCAACTCCCCATATGCACAACCAATGCCTTTCCCTCCAAACTATTCCAATCCATCTGCAGTCGGACCAAGTTACCCACCAGCCAGCGCAACCAGTTACCCGCCCGCAGGCGGACCAAACTACCTGCCAGCAAACAGACCAAGCTACCCCCCAGCAGGGGGGCCAAGCTACCCACCAGCAGGGGGGCCAAGCTACCCACCAGCAGGCGGGCCGAGCTATCAGCCGCCTAGAACTCCATCACCGCCTCCCCCGCCTTCTAATGTTGGCTACATACCTACTTTTATCCCGAGAACAGATCGAATGCAGGAGACATATATCAATATGCCAGCATCTGGAGCACCGCCGGGCCGTAGAGGAGCAGCTGGTGTTGGAATGGGAATCGGTGCTGGAGCGCTGGCGGCAGGTGCTGTGATCTTTGGTGATGACTTCATGTCAGGATTCGATGTTCCTTCTGGCTTACAAGATGCTAGTCTTACCATATCAACTGATCCTCCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

358

Amino Acids

38.16

Weight (kDa)

8.39

Isoelectric Point (pI)

64.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 106 4.1e-07 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014972)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40815 AT2G40815
fragaria_vesca FvH4_7g26311
malus_domestica MD01G1172000.v1.1 MD07G1238500.v1.1
prunus_persica Prupe.2G267300_v2.0.a1
pyrus_communis pycom07g21450
rosa_chinensis RchiOBHm_Chr1g0372771
rosa_laevigata RLG00000026873
rosa_multiflora Rmu_sc0029212.1_g000001
rosa_rugosa Rorug01G0042900 Rorug01G0374300
rosa_samantha Rh1AG383000 Rh1CG359900 Rh1DG377600
rosa_wichuraiana Rw1G033710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 965
Acc36I ACCTGC 2 cut(s) 705, 965
AccB7I CCANNNNNTGG 1 cut(s) 905
AclWI GGATC 3 cut(s) 216, 523, 1055
AcsI RAATTY 1 cut(s) 379
AcuI CTGAAG 2 cut(s) 251, 366
AfaI GTAC 3 cut(s) 116, 233, 452
AfeI AGCGCT 1 cut(s) 966
AgsI TTSAA 1 cut(s) 20
AhdI GACNNNNNGTC 1 cut(s) 1003
AloI GAACNNNNNNTCC 2 cut(s) 1002, 1034
Alw21I GWGCWC 1 cut(s) 913
Alw26I GTCTC 3 cut(s) 415, 504, 875
AlwI GGATC 3 cut(s) 216, 523, 1055
AlwNI CAGNNNCTG 3 cut(s) 211, 905, 980
Ama87I CYCGRG 2 cut(s) 39, 859
Aor51HI AGCGCT 1 cut(s) 966
AoxI GGCC 5 cut(s) 554, 734, 758, 782, 919
ApeKI GCWGC 2 cut(s) 217, 932
ApoI RAATTY 1 cut(s) 379
Asp700I GAANNNNTTC 1 cut(s) 264
AspLEI GCGC 3 cut(s) 217, 662, 967
AspS9I GGNCC 7 cut(s) 470, 638, 686, 734, 758, 782, 919
AsuC2I CCSGG 2 cut(s) 257, 918
AsuHPI GGTGA 3 cut(s) 487, 804, 1004
AvaI CYCGRG 2 cut(s) 39, 859
AvaII GGWCC 3 cut(s) 470, 638, 686
BanII GRGCYC 2 cut(s) 48, 191
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 913
BbvI GCAGC 2 cut(s) 204, 944
BccI CCATC 2 cut(s) 634, 817
BceAI ACGGC 2 cut(s) 291, 906
BcnI CCSGG 2 cut(s) 257, 918
BcoDI GTCTC 3 cut(s) 415, 504, 875
BfaI CTAG 2 cut(s) 801, 1041
BfmI CTRYAG 2 cut(s) 537, 630
BfoI RGCGCY 2 cut(s) 218, 968
BfuAI ACCTGC 2 cut(s) 705, 965
BisI GCNGC 4 cut(s) 218, 797, 933, 972
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 4 cut(s) 219, 798, 934, 973
BmcAI AGTACT 1 cut(s) 116
Bme1390I CCNGG 2 cut(s) 257, 918
Bme18I GGWCC 3 cut(s) 470, 638, 686
BmeRI GACNNNNNGTC 1 cut(s) 1003
BmeT110I CYCGRG 2 cut(s) 39, 859
BmgT120I GGNCC 7 cut(s) 470, 638, 686, 734, 758, 782, 919
BmiI GGNNCC 3 cut(s) 330, 735, 759
BmrFI CCNGG 2 cut(s) 257, 918
BmsI GCATC 3 cut(s) 121, 911, 1027
BpiI GAAGAC 1 cut(s) 51
BpmI CTGGAG 3 cut(s) 59, 927, 981
Bpu10I CCTNAGC 1 cut(s) 47
Bpu1102I GCTNAGC 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 378
BpuMI CCSGG 2 cut(s) 257, 918
BsaAI YACGTR 1 cut(s) 419
BsaJI CCNNGG 3 cut(s) 40, 332, 498
BsaWI WCCGGW 2 cut(s) 151, 506
BsaXI ACNNNNNCTCC 1 cut(s) 1048
Bse1I ACTGG 3 cut(s) 76, 364, 666
BseDI CCNNGG 3 cut(s) 40, 332, 498
BseMII CTCAG 4 cut(s) 61, 281, 315, 417
BseNI ACTGG 3 cut(s) 76, 364, 666
BseRI GAGGAG 1 cut(s) 942
BseXI GCAGC 2 cut(s) 204, 944
BseYI CCCAGC 1 cut(s) 724
BsgI GTGCAG 3 cut(s) 103, 203, 504
BshFI GGCC 5 cut(s) 556, 736, 760, 784, 921
BsiHKAI GWGCWC 1 cut(s) 913
BsiHKCI CYCGRG 2 cut(s) 39, 859
BsiSI CCGG 5 cut(s) 152, 256, 507, 557, 917
BslFI GGGAC 1 cut(s) 286
BsmAI GTCTC 3 cut(s) 415, 504, 875
BsmBI CGTCTC 1 cut(s) 504
BsmFI GGGAC 1 cut(s) 286
BsmI GAATGC 1 cut(s) 879
BsnI GGCC 5 cut(s) 556, 736, 760, 784, 921
BsoBI CYCGRG 2 cut(s) 39, 859
Bsp1286I GDGCHC 3 cut(s) 48, 191, 913
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 6 cut(s) 208, 515, 562, 868, 984, 1060
Bsp1720I GCTNAGC 1 cut(s) 306
BspANI GGCC 5 cut(s) 556, 736, 760, 784, 921
BspCNI CTCAG 4 cut(s) 60, 282, 316, 418
BspLI GGNNCC 3 cut(s) 330, 735, 759
BspMAI CTGCAG 1 cut(s) 634
BspMI ACCTGC 2 cut(s) 705, 965
BspPI GGATC 3 cut(s) 216, 523, 1055
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 3 cut(s) 76, 364, 666
BssECI CCNNGG 3 cut(s) 40, 332, 498
BssMI GATC 6 cut(s) 208, 515, 562, 868, 984, 1060
BssT1I CCWWGG 1 cut(s) 498
BstAUI TGTACA 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 419
BstC8I GCNNGC 8 cut(s) 658, 678, 682, 702, 778, 782, 900, 969
BstDEI CTNAG 5 cut(s) 47, 290, 306, 324, 426
BstH2I RGCGCY 2 cut(s) 218, 968
BstHHI GCGC 3 cut(s) 217, 662, 967
BstKTI GATC 6 cut(s) 211, 518, 565, 871, 987, 1063
BstMAI GTCTC 3 cut(s) 415, 504, 875
BstMBI GATC 6 cut(s) 208, 515, 562, 868, 984, 1060
BstMWI GCNNNNNNNGC 5 cut(s) 786, 823, 908, 971, 977
BstSCI CCNGG 2 cut(s) 255, 916
BstSFI CTRYAG 2 cut(s) 537, 630
BstSNI TACGTA 1 cut(s) 419
BstV1I GCAGC 2 cut(s) 204, 944
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 2 cut(s) 208, 515
BstYI RGATCY 2 cut(s) 208, 515
BsuRI GGCC 5 cut(s) 556, 736, 760, 784, 921
BtsI GCAGTG 1 cut(s) 466
BtsIMutI CAGTG 2 cut(s) 83, 466
BveI ACCTGC 2 cut(s) 705, 965
Cac8I GCNNGC 8 cut(s) 658, 678, 682, 702, 778, 782, 900, 969
CaiI CAGNNNCTG 3 cut(s) 211, 905, 980
CfoI GCGC 3 cut(s) 217, 662, 967
Cfr13I GGNCC 7 cut(s) 470, 638, 686, 734, 758, 782, 919
Csp6I GTAC 3 cut(s) 115, 232, 451
CviAII CATG 2 cut(s) 338, 1003
CviQI GTAC 3 cut(s) 115, 232, 451
DdeI CTNAG 5 cut(s) 47, 290, 306, 324, 426
DpnI GATC 6 cut(s) 210, 517, 564, 870, 986, 1062
DpnII GATC 6 cut(s) 208, 515, 562, 868, 984, 1060
DriI GACNNNNNGTC 1 cut(s) 1003
Eam1105I GACNNNNNGTC 1 cut(s) 1003
EciI GGCGGA 3 cut(s) 25, 212, 699
Eco105I TACGTA 1 cut(s) 419
Eco130I CCWWGG 1 cut(s) 498
Eco24I GRGCYC 2 cut(s) 48, 191
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 3 cut(s) 470, 638, 686
Eco47III AGCGCT 1 cut(s) 966
Eco57I CTGAAG 2 cut(s) 251, 366
Eco88I CYCGRG 2 cut(s) 39, 859
EcoO109I RGGNCCY 1 cut(s) 470
EcoRI GAATTC 1 cut(s) 379
EcoRV GATATC 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 498
EcoT38I GRGCYC 2 cut(s) 48, 191
ErhI CCWWGG 1 cut(s) 498
Esp3I CGTCTC 1 cut(s) 504
FaeI CATG 2 cut(s) 341, 1006
FaqI GGGAC 1 cut(s) 286
FatI CATG 2 cut(s) 337, 1002
FauI CCCGC 4 cut(s) 681, 685, 773, 831
FauNDI CATATG 1 cut(s) 586
Fnu4HI GCNGC 4 cut(s) 218, 797, 933, 972
FriOI GRGCYC 2 cut(s) 48, 191
Fsp4HI GCNGC 4 cut(s) 218, 797, 933, 972
FspBI CTAG 2 cut(s) 801, 1041
GlaI GCGC 3 cut(s) 216, 661, 966
GluI GCNGC 4 cut(s) 218, 797, 933, 972
GsaI CCCAGC 1 cut(s) 728
GsuI CTGGAG 3 cut(s) 59, 927, 981
HaeII RGCGCY 2 cut(s) 218, 968
HaeIII GGCC 5 cut(s) 556, 736, 760, 784, 921
HapII CCGG 5 cut(s) 152, 256, 507, 557, 917
HhaI GCGC 3 cut(s) 217, 662, 967
Hin1II CATG 2 cut(s) 341, 1006
Hin6I GCGC 3 cut(s) 215, 660, 965
HinP1I GCGC 3 cut(s) 215, 660, 965
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 3 cut(s) 148, 951, 1011
HpaII CCGG 5 cut(s) 152, 256, 507, 557, 917
HphI GGTGA 3 cut(s) 487, 804, 1004
Hpy166II GTNNAC 3 cut(s) 96, 232, 453
Hpy188I TCNGA 7 cut(s) 105, 193, 325, 333, 427, 638, 1073
Hpy188III TCNNGA 5 cut(s) 138, 289, 859, 906, 1008
Hpy8I GTNNAC 3 cut(s) 96, 232, 453
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 3 cut(s) 458, 837, 1032
HpyCH4IV ACGT 2 cut(s) 222, 418
HpyCH4V TGCA 7 cut(s) 120, 205, 220, 521, 590, 632, 877
HpyF10VI GCNNNNNNNGC 5 cut(s) 786, 823, 908, 971, 977
HpyF3I CTNAG 5 cut(s) 47, 290, 306, 324, 426
HpySE526I ACGT 2 cut(s) 222, 418
Hsp92II CATG 2 cut(s) 341, 1006
HspAI GCGC 3 cut(s) 215, 660, 965
Kzo9I GATC 6 cut(s) 208, 515, 562, 868, 984, 1060
LmnI GCTCC 7 cut(s) 78, 334, 341, 357, 908, 929, 962
Lsp1109I GCAGC 2 cut(s) 204, 944
LweI GCATC 3 cut(s) 121, 911, 1027
MaeI CTAG 2 cut(s) 801, 1041
MaeII ACGT 2 cut(s) 222, 418
MaeIII GTNAC 2 cut(s) 644, 668
MalI GATC 6 cut(s) 210, 517, 564, 870, 986, 1062
MboI GATC 6 cut(s) 208, 515, 562, 868, 984, 1060
MboII GAAGA 4 cut(s) 51, 241, 359, 572
MflI RGATCY 2 cut(s) 208, 515
MhlI GDGCHC 3 cut(s) 48, 191, 913
MluCI AATT 2 cut(s) 379, 566
MmeI TCCRAC 3 cut(s) 408, 616, 922
MroXI GAANNNNTTC 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 935
MspI CCGG 5 cut(s) 152, 256, 507, 557, 917
MspR9I CCNGG 2 cut(s) 257, 918
Mva1269I GAATGC 1 cut(s) 879
MwoI GCNNNNNNNGC 5 cut(s) 786, 823, 908, 971, 977
NciI CCSGG 2 cut(s) 257, 918
NdeI CATATG 1 cut(s) 586
NdeII GATC 6 cut(s) 208, 515, 562, 868, 984, 1060
NlaIII CATG 2 cut(s) 341, 1006
NlaIV GGNNCC 3 cut(s) 330, 735, 759
NmeAIII GCCGAG 1 cut(s) 810
PaqCI CACCTGC 1 cut(s) 965
PctI GAATGC 1 cut(s) 879
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 3 cut(s) 148, 951, 1011
PflMI CCANNNNNTGG 1 cut(s) 905
PfoI TCCNGGA 1 cut(s) 255
PkrI GCNGC 4 cut(s) 219, 798, 934, 973
Ppu21I YACGTR 1 cut(s) 419
PpuMI RGGWCCY 1 cut(s) 470
Psp5II RGGWCCY 1 cut(s) 470
PspFI CCCAGC 1 cut(s) 724
PspN4I GGNNCC 3 cut(s) 330, 735, 759
PspPI GGNCC 7 cut(s) 470, 638, 686, 734, 758, 782, 919
PspPPI RGGWCCY 1 cut(s) 470
PstI CTGCAG 1 cut(s) 634
PstNI CAGNNNCTG 3 cut(s) 211, 905, 980
PsuI RGATCY 2 cut(s) 208, 515
PvuII CAGCTG 1 cut(s) 935
RsaI GTAC 3 cut(s) 116, 233, 452
RsaNI GTAC 3 cut(s) 115, 232, 451
SatI GCNGC 4 cut(s) 218, 797, 933, 972
Sau3AI GATC 6 cut(s) 208, 515, 562, 868, 984, 1060
Sau96I GGNCC 7 cut(s) 470, 638, 686, 734, 758, 782, 919
ScaI AGTACT 1 cut(s) 116
ScrFI CCNGG 2 cut(s) 257, 918
SduI GDGCHC 3 cut(s) 48, 191, 913
SfaNI GCATC 3 cut(s) 121, 911, 1027
SfcI CTRYAG 2 cut(s) 537, 630
SinI GGWCC 3 cut(s) 470, 638, 686
SmlI CTYRAG 1 cut(s) 393
SmoI CTYRAG 1 cut(s) 393
SnaBI TACGTA 1 cut(s) 419
Sse9I AATT 2 cut(s) 379, 566
SspMI CTAG 2 cut(s) 801, 1041
StyD4I CCNGG 2 cut(s) 255, 916
StyI CCWWGG 1 cut(s) 498
TaiI ACGT 2 cut(s) 225, 421
TaqI TCGA 5 cut(s) 405, 442, 544, 871, 1014
TasI AATT 2 cut(s) 379, 566
TatI WGTACW 2 cut(s) 114, 231
TauI GCSGC 2 cut(s) 799, 974
TfiI GAWTC 3 cut(s) 148, 951, 1011
TscAI CASTG 2 cut(s) 83, 466
TseI GCWGC 2 cut(s) 217, 932
TspDTI ATGAA 1 cut(s) 991
TspGWI ACGGA 2 cut(s) 528, 560
TspRI CASTG 2 cut(s) 83, 466
Van91I CCANNNNNTGG 1 cut(s) 905
VpaK11BI GGWCC 3 cut(s) 470, 638, 686
XapI RAATTY 1 cut(s) 379
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 2 cut(s) 801, 1041
ZrmI AGTACT 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.