FvH4_7g28501

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013557
Physical Location & Seq
Forward (+)
21128660 .. 21131782
3123 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g28501.t1

Sequence Viewer

Length: 2337 bp
ATGAAAATGGTTGAAAAACCAGTGATCAAATGGCTGCTGGTTCTGATTCTAGCAAGCTCTGTCTTCTTCTTCACCAATGCCCTCTCTGCAAAAACCCAAACTTACATCATCCAAATGGACAGGTCTGCCAAGCCTGAATCTTTCAACTCCCACTTAGATTGGTACTCATCCAAAGTAAACTCAGTACTCACCAAACCAGATAAAGCAGATGATCAGAACCAAAACCAAGACAGAATCATCTACACTTACCAAACCGCCTTCCACGGCGTCGCAGCTCAACTGACCCAACAAGAAGCTCAACTACTAGAGCAACAAGACTCCGTCTTGTCTATATTCCCAGAACAAAAGTACGAGCTCCACACGACCAGAAGTCCTCTGTTTCTGGGACTCGAGCCACACGACAGCACCACCGCCAACGTCTGGTCCCAGCGCCTCTCCGACCATGACGTCATCGTCGGAGTACTCGACACAGGTGTCTGGCCGGAGAGCCAGAGCTTCAACGACACCGGGATGTCTCCAGTGCCGCGGCGGTGGCGCGGCGCGTGCGAGACCGGGAGAGGCTTCACGAAGCTGAACTGCAACCGGAAAGTCGTCGGCGCCAGAATCTTCCTCCGGGGGTACGAGTCGGCCACCGGAAAGTTCAACGAGAAGACTGAGTACAAGTCGCCGAGAGACCAGGACGGACATGGGACCCACACCGCCGCGACCGTCGCCGGGTCCGCAGTGCGCGGCGCCAATCTTCTCGGCTACGCTTACGGCACGGCGAGGGGGATGGCGCCGGCGGCGAGGATCGCGGCGTACAAGGTGTGCTGGATGGGGGGTTGTTTCAGCTCCGACATAATGGCGGCGGTTGATAAGGCCGTAGCGGACGGCGTCGATGTTTTGTCTATTTCACTTGGTGGTGGGGTTTCCTCGTACTACCGGGATAGCTTGTCTATTGCGGCGTTTGGGGCTATGGAGATGGGTGTTTTTGTTTCCTGCTCCGCCGGGAACGGTGGGCCAGACCCGGTGAGTTTGACTAATGTGTCGCCGTGGATCACCACCGTCGGTGCGAGCACCATGGATAGAGATTTTCCCGCCATTGTGAAGTTGGGAAATGGGAGGAGTATTACTGGTGTTTCGCTTTATAGAGGTAGAATGAAGCTTGATACAAAGAAGATGTATCCTGTTGTGTTTGCGGGTGGGAATTCGAGTAGTCCGGATCCGAGTTCGTTGTGTTTAGAAGGTACTTTGAATCCAAGGAAAGTTAAGGGGAAGATTGTGATTTGTGAGCGTGGGATTAGTGCTAGAGTGCAGAAGGGAGTGGTGGTGAAGAATGCAGGAGGAGTTGGGATGATCTTGGCGAATACGGCTTCAAATGGAGAGGAGCTTGTGGCTGATTGTCATCTCATTCCGGCGGTTGCTGTTGGAGAGAATGAAGCGAAATTGATCAAGCATTATGCTTTAAGTAGTCCGAGAGCAAAGGCAACTCTAGCATTTGTTGGTACTAGAGTTGGGATTAGGCCTTCTCCTGTTGTGGCTGCATTTTCGTCAAGAGGGCCTAATCTGTTGAGTTTTGAGATTATGAAGCCCGATTTGGTTGCGCCTGGAGTGAACATTCTTGCTGCTTGGACCGGAGAATTGGGTCCGTCGAGCTTGGCAACTGACCGCAGGAGAGTGAAGTTCAATATCTTGTCTGGGACATCAATGTCATGTCCTCATGTGAGTGGGATTGCTGCTTTGCTCAAGTCTAGGCACCCAGAATGGAGTCCAGCAGCGATCAAATCCGCGCTAATGACTACAGCTTATGTTCATGACAACACAAATAAGCCTCTTGAAGATGCATCAAGAGCTGAGATTTCAACCCCATTTGATCATGGAGCTGGACACATAAACCCTAGCAAGGCTCTTGACCCGGGTTTGGTTTATGACATTCAGCCTCAGGATTATCTTGAGTTCCTCTGCACACAAAAGTTAACCCCAATGCAGCTCAAGGTGTTTAGCAATAGGACTTGCCGTCACACTCTAGCCAATGCAGGGGACCTCAACTATCCAGCCATTTCGGTTGTGTTTGTGGAGAACACTAATGTATCTGTCTTGACAGTTCATAGAACTGTCACTAACGTTGGCCCTGCAGTTTCGAGCTACCATGCCATTGTTTCGCCATTCAAGGGTGCTTACGTGAAAGTTGAGCCGCGGATACTGAAGTTCACCAAAGCTAATCAGAAGCTGTCTTACAAGGTGACCTTTACTACAAAGACTAGGCAGCCAGAACCCGAGTTTGGAGGCTTGGTGTGGAAAGATGGAGTGCACAGAGTGAGAAGTCCAATTGTTGTGGCATGGGGTATGCTGATATGA

Protein Analysis

779

Amino Acids

84.12

Weight (kDa)

9.29

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 120 4.5e-20 Peptidase inhibitor I9
Peptidase_S8 PF00082 148 - 601 3.5e-51 Subtilase family
PA PF02225 388 - 476 6.6e-13 PA domain
fn3_6 PF17766 674 - 772 2e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013557)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51750
fragaria_vesca FvH4_7g28501
malus_domestica MD01G1188800.v1.1 MD01G1189100.v1.1 MD01G1189900.v1.1 MD01G1190400.v1.1
prunus_persica Prupe.2G285500_v2.0.a1
pyrus_communis pycom01g20050
rosa_chinensis RchiOBHm_Chr1g0375891
rosa_laevigata RLG00000026646
rosa_multiflora Rmu_sc0001943.1_g000010
rosa_roxburghii Rroxscaffold_4G00282550
rosa_rugosa Rorug01G0392200
rosa_samantha Rh1AG404100 Rh1BG367500 Rh1CG380300 Rh1DG397500
rosa_wichuraiana Rw0G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 5 cut(s) 446, 452, 473, 1024, 1687
AatII GACGTC 1 cut(s) 450
AccB1I GGYRCC 4 cut(s) 596, 731, 775, 1734
AccB7I CCANNNNNTGG 1 cut(s) 420
AccII CGCG 8 cut(s) 526, 537, 542, 704, 729, 794, 1769, 2176
AccIII TCCGGA 1 cut(s) 1198
AclI AACGTT 1 cut(s) 2103
AclWI GGATC 4 cut(s) 797, 1043, 1196, 1209
AcoI YGGCCR 2 cut(s) 479, 627
AcsI RAATTY 1 cut(s) 1186
AcuI CTGAAG 1 cut(s) 2204
AcyI GRCGYC 6 cut(s) 267, 447, 597, 732, 776, 873
AdeI CACNNNGTG 2 cut(s) 899, 2296
AfiI CCNNNNNNNGG 7 cut(s) 420, 714, 1882, 1900, 2016, 2150, 2261
AhdI GACNNNNNGTC 2 cut(s) 369, 1995
AjnI CCWGG 2 cut(s) 675, 1585
AjuI GAANNNNNNNTTGG 4 cut(s) 1559, 1591, 2244, 2276
Alw21I GWGCWC 3 cut(s) 357, 1058, 2292
Alw26I GTCTC 3 cut(s) 519, 542, 666
Alw44I GTGCAC 1 cut(s) 2288
AlwI GGATC 4 cut(s) 797, 1043, 1196, 1209
AlwNI CAGNNNCTG 1 cut(s) 2209
Ama87I CYCGRG 3 cut(s) 389, 1894, 2255
Aor13HI TCCGGA 1 cut(s) 1198
AoxI GGCC 7 cut(s) 479, 627, 858, 998, 1502, 1538, 2107
ApaLI GTGCAC 1 cut(s) 2288
ApeKI GCWGC 8 cut(s) 34, 272, 1520, 1604, 1715, 1754, 1966, 2245
ApoI RAATTY 1 cut(s) 1186
AspLEI GCGC 9 cut(s) 432, 537, 542, 599, 729, 734, 778, 1585, 1771
AspS9I GGNCC 9 cut(s) 423, 690, 717, 998, 1538, 1611, 1625, 2020, 2108
AsuC2I CCSGG 9 cut(s) 508, 553, 614, 715, 923, 988, 1007, 1895, 1896
AsuHPI GGTGA 7 cut(s) 64, 181, 1021, 1030, 1321, 2182, 2233
AvaI CYCGRG 3 cut(s) 389, 1894, 2255
AvaII GGWCC 6 cut(s) 423, 690, 717, 1611, 1625, 2020
AxyI CCTNAGG 1 cut(s) 1920
BaeGI GKGCMC 1 cut(s) 2292
BaeI ACNNNNGTAYC 6 cut(s) 1476, 1509, 2052, 2085, 2171, 2204
BamHI GGATCC 1 cut(s) 1201
BanI GGYRCC 4 cut(s) 596, 731, 775, 1734
BanII GRGCYC 1 cut(s) 357
BarI GAAGNNNNNNTAC 6 cut(s) 285, 317, 641, 673, 2198, 2230
BbsI GAAGAC 2 cut(s) 55, 656
Bbv12I GWGCWC 3 cut(s) 357, 1058, 2292
BbvI GCAGC 8 cut(s) 21, 284, 1507, 1591, 1702, 1766, 1978, 2257
BccI CCATC 4 cut(s) 766, 808, 955, 2276
BceAI ACGGC 8 cut(s) 280, 772, 777, 845, 886, 1015, 1365, 1980
BcgI CGANNNNNNTGC 2 cut(s) 1562, 1596
BciT130I CCWGG 2 cut(s) 677, 1587
BciVI GTATCC 2 cut(s) 1173, 2172
BclI TGATCA 4 cut(s) 24, 211, 1428, 1852
BcnI CCSGG 9 cut(s) 508, 553, 614, 715, 923, 988, 1007, 1895, 1896
BcoDI GTCTC 3 cut(s) 519, 542, 666
BfaI CTAG 9 cut(s) 50, 305, 1287, 1472, 1488, 1731, 1878, 2006, 2241
BfmI CTRYAG 2 cut(s) 1779, 2112
BfoI RGCGCY 4 cut(s) 433, 600, 735, 779
BfuI GTATCC 2 cut(s) 1173, 2172
BmcAI AGTACT 2 cut(s) 186, 462
Bme18I GGWCC 6 cut(s) 423, 690, 717, 1611, 1625, 2020
BmeRI GACNNNNNGTC 2 cut(s) 369, 1995
BmeT110I CYCGRG 3 cut(s) 389, 1894, 2255
BmgT120I GGNCC 9 cut(s) 423, 690, 717, 998, 1538, 1611, 1625, 2020, 2108
BmsI GCATC 2 cut(s) 1810, 1832
BpiI GAAGAC 2 cut(s) 55, 656
BpmI CTGGAG 2 cut(s) 501, 1608
BpuEI CTTGAG 3 cut(s) 1709, 1952, 1955
BpuMI CCSGG 9 cut(s) 508, 553, 614, 715, 923, 988, 1007, 1895, 1896
BsaAI YACGTR 1 cut(s) 2161
BsaBI GATNNNNATC 1 cut(s) 1383
BsaHI GRCGYC 6 cut(s) 267, 447, 597, 732, 776, 873
BsaI GGTCTC 2 cut(s) 542, 666
BsaJI CCNNGG 8 cut(s) 262, 524, 613, 1031, 1059, 1238, 1894, 2174
BsaWI WCCGGW 4 cut(s) 582, 632, 1198, 1613
BsaXI ACNNNNNCTCC 4 cut(s) 1293, 1323, 2256, 2286
Bsc4I CCNNNNNNNGG 7 cut(s) 420, 714, 1882, 1900, 2016, 2150, 2261
Bse118I RCCGGY 1 cut(s) 778
Bse1I ACTGG 3 cut(s) 20, 518, 1117
Bse21I CCTNAGG 1 cut(s) 1920
Bse8I GATNNNNATC 1 cut(s) 1383
BseAI TCCGGA 1 cut(s) 1198
BseBI CCWGG 2 cut(s) 677, 1587
BseDI CCNNGG 8 cut(s) 262, 524, 613, 1031, 1059, 1238, 1894, 2174
BseGI GGATG 6 cut(s) 108, 167, 516, 777, 819, 1338
BseJI GATNNNNATC 1 cut(s) 1383
BseLI CCNNNNNNNGG 7 cut(s) 420, 714, 1882, 1900, 2016, 2150, 2261
BseMII CTCAG 4 cut(s) 195, 645, 1824, 1934
BseNI ACTGG 3 cut(s) 20, 518, 1117
BseRI GAGGAG 3 cut(s) 1117, 1338, 1379
BseSI GKGCMC 1 cut(s) 2292
BseXI GCAGC 8 cut(s) 21, 284, 1507, 1591, 1702, 1766, 1978, 2257
BseYI CCCAGC 1 cut(s) 426
BsgI GTGCAG 2 cut(s) 1313, 1927
Bsh1236I CGCG 8 cut(s) 526, 537, 542, 704, 729, 794, 1769, 2176
Bsh1285I CGRYCG 1 cut(s) 708
BshFI GGCC 7 cut(s) 481, 629, 860, 1000, 1504, 1540, 2109
BshNI GGYRCC 4 cut(s) 596, 731, 775, 1734
BsiEI CGRYCG 1 cut(s) 708
BsiHKAI GWGCWC 3 cut(s) 357, 1058, 2292
BsiHKCI CYCGRG 3 cut(s) 389, 1894, 2255
BslFI GGGAC 5 cut(s) 399, 409, 703, 1693, 2033
BslI CCNNNNNNNGG 7 cut(s) 420, 714, 1882, 1900, 2016, 2150, 2261
BsmAI GTCTC 3 cut(s) 519, 542, 666
BsmFI GGGAC 5 cut(s) 399, 409, 703, 1693, 2033
BsmI GAATGC 1 cut(s) 1321
BsnI GGCC 7 cut(s) 481, 629, 860, 1000, 1504, 1540, 2109
Bso31I GGTCTC 2 cut(s) 542, 666
BsoBI CYCGRG 3 cut(s) 389, 1894, 2255
Bsp1286I GDGCHC 3 cut(s) 357, 1058, 2292
Bsp13I TCCGGA 1 cut(s) 1198
Bsp143I GATC 9 cut(s) 24, 211, 789, 1035, 1201, 1335, 1428, 1758, 1852
Bsp19I CCATGG 1 cut(s) 1059
BspANI GGCC 7 cut(s) 481, 629, 860, 1000, 1504, 1540, 2109
BspCNI CTCAG 4 cut(s) 194, 646, 1825, 1933
BspEI TCCGGA 1 cut(s) 1198
BspFNI CGCG 8 cut(s) 526, 537, 542, 704, 729, 794, 1769, 2176
BspHI TCATGA 1 cut(s) 1792
BspMAI CTGCAG 1 cut(s) 2116
BspPI GGATC 4 cut(s) 797, 1043, 1196, 1209
BspT107I GGYRCC 4 cut(s) 596, 731, 775, 1734
BspTNI GGTCTC 2 cut(s) 542, 666
BsrFI RCCGGY 1 cut(s) 778
BsrI ACTGG 3 cut(s) 20, 518, 1117
BssAI RCCGGY 1 cut(s) 778
BssECI CCNNGG 8 cut(s) 262, 524, 613, 1031, 1059, 1238, 1894, 2174
BssMI GATC 9 cut(s) 24, 211, 789, 1035, 1201, 1335, 1428, 1758, 1852
BssNI GRCGYC 6 cut(s) 267, 447, 597, 732, 776, 873
BssT1I CCWWGG 2 cut(s) 1059, 1238
Bst2UI CCWGG 2 cut(s) 677, 1587
Bst4CI ACNGT 5 cut(s) 709, 995, 1045, 2083, 2095
BstACI GRCGYC 6 cut(s) 267, 447, 597, 732, 776, 873
BstBAI YACGTR 1 cut(s) 2161
BstC8I GCNNGC 4 cut(s) 55, 544, 780, 1054
BstDEI CTNAG 5 cut(s) 154, 181, 654, 1833, 1920
BstDSI CCRYGG 5 cut(s) 262, 524, 1031, 1059, 2174
BstEII GGTNACC 1 cut(s) 2221
BstF5I GGATG 6 cut(s) 108, 167, 516, 777, 819, 1338
BstFNI CGCG 8 cut(s) 526, 537, 542, 704, 729, 794, 1769, 2176
BstH2I RGCGCY 4 cut(s) 433, 600, 735, 779
BstHHI GCGC 9 cut(s) 432, 537, 542, 599, 729, 734, 778, 1585, 1771
BstKTI GATC 9 cut(s) 27, 214, 792, 1038, 1204, 1338, 1431, 1761, 1855
BstMAI GTCTC 3 cut(s) 519, 542, 666
BstMBI GATC 9 cut(s) 24, 211, 789, 1035, 1201, 1335, 1428, 1758, 1852
BstMCI CGRYCG 1 cut(s) 708
BstNI CCWGG 2 cut(s) 677, 1587
BstPI GGTNACC 1 cut(s) 2221
BstSFI CTRYAG 2 cut(s) 1779, 2112
BstSLI GKGCMC 1 cut(s) 2292
BstUI CGCG 8 cut(s) 526, 537, 542, 704, 729, 794, 1769, 2176
BstV1I GCAGC 8 cut(s) 21, 284, 1507, 1591, 1702, 1766, 1978, 2257
BstV2I GAAGAC 2 cut(s) 55, 656
BstX2I RGATCY 1 cut(s) 1201
BstYI RGATCY 1 cut(s) 1201
Bsu36I CCTNAGG 1 cut(s) 1920
BsuI GTATCC 2 cut(s) 1173, 2172
BsuRI GGCC 7 cut(s) 481, 629, 860, 1000, 1504, 1540, 2109
BtgI CCRYGG 5 cut(s) 262, 524, 1031, 1059, 2174
BtsCI GGATG 6 cut(s) 108, 167, 516, 777, 819, 1338
BtsI GCAGTG 1 cut(s) 729
BtsIMutI CAGTG 3 cut(s) 27, 525, 729
Cac8I GCNNGC 4 cut(s) 55, 544, 780, 1054
CaiI CAGNNNCTG 1 cut(s) 2209
CciI TCATGA 1 cut(s) 1792
CfoI GCGC 9 cut(s) 432, 537, 542, 599, 729, 734, 778, 1585, 1771
Cfr10I RCCGGY 1 cut(s) 778
Cfr13I GGNCC 9 cut(s) 423, 690, 717, 998, 1538, 1611, 1625, 2020, 2108
Cfr42I CCGCGG 2 cut(s) 527, 2177
Cfr9I CCCGGG 1 cut(s) 1894
CseI GACGC 2 cut(s) 256, 862
CspCI CAANNNNNGTGG 4 cut(s) 140, 175, 2295, 2330
CviAII CATG 9 cut(s) 443, 686, 1060, 1692, 1700, 1793, 1856, 2129, 2319
DdeI CTNAG 5 cut(s) 154, 181, 654, 1833, 1920
DinI GGCGCC 3 cut(s) 598, 733, 777
DpnI GATC 9 cut(s) 26, 213, 791, 1037, 1203, 1337, 1430, 1760, 1854
DpnII GATC 9 cut(s) 24, 211, 789, 1035, 1201, 1335, 1428, 1758, 1852
DraIII CACNNNGTG 2 cut(s) 899, 2296
DrdI GACNNNNNNGTC 5 cut(s) 446, 452, 473, 1024, 1687
DriI GACNNNNNGTC 2 cut(s) 369, 1995
DseDI GACNNNNNNGTC 5 cut(s) 446, 452, 473, 1024, 1687
EaeI YGGCCR 2 cut(s) 479, 627
Eam1105I GACNNNNNGTC 2 cut(s) 369, 1995
EciI GGCGGA 1 cut(s) 973
Ecl136II GAGCTC 1 cut(s) 355
Eco130I CCWWGG 2 cut(s) 1059, 1238
Eco147I AGGCCT 1 cut(s) 1504
Eco24I GRGCYC 1 cut(s) 357
Eco31I GGTCTC 2 cut(s) 542, 666
Eco47I GGWCC 6 cut(s) 423, 690, 717, 1611, 1625, 2020
Eco53kI GAGCTC 1 cut(s) 355
Eco57I CTGAAG 1 cut(s) 2204
Eco81I CCTNAGG 1 cut(s) 1920
Eco88I CYCGRG 3 cut(s) 389, 1894, 2255
Eco91I GGTNACC 1 cut(s) 2221
EcoICRI GAGCTC 1 cut(s) 355
EcoO109I RGGNCCY 3 cut(s) 690, 1538, 2020
EcoO65I GGTNACC 1 cut(s) 2221
EcoRI GAATTC 1 cut(s) 1186
EcoRII CCWGG 2 cut(s) 675, 1585
EcoT14I CCWWGG 2 cut(s) 1059, 1238
EcoT22I ATGCAT 1 cut(s) 1825
EcoT38I GRGCYC 1 cut(s) 357
EgeI GGCGCC 3 cut(s) 598, 733, 777
EheI GGCGCC 3 cut(s) 598, 733, 777
ErhI CCWWGG 2 cut(s) 1059, 1238
FaeI CATG 9 cut(s) 446, 689, 1063, 1695, 1703, 1796, 1859, 2132, 2322
FalI AAGNNNNNCTT 2 cut(s) 2210, 2242
FaqI GGGAC 5 cut(s) 399, 409, 703, 1693, 2033
FatI CATG 9 cut(s) 442, 685, 1059, 1691, 1699, 1792, 1855, 2128, 2318
FauI CCCGC 2 cut(s) 1084, 1171
FbaI TGATCA 4 cut(s) 24, 211, 1428, 1852
FokI GGATG 6 cut(s) 95, 154, 523, 784, 826, 1345
FriOI GRGCYC 1 cut(s) 357
FspBI CTAG 9 cut(s) 50, 305, 1287, 1472, 1488, 1731, 1878, 2006, 2241
GlaI GCGC 9 cut(s) 431, 536, 541, 598, 728, 733, 777, 1584, 1770
GsaI CCCAGC 1 cut(s) 430
GsuI CTGGAG 2 cut(s) 501, 1608
HaeII RGCGCY 4 cut(s) 433, 600, 735, 779
HaeIII GGCC 7 cut(s) 481, 629, 860, 1000, 1504, 1540, 2109
HgaI GACGC 2 cut(s) 256, 862
HhaI GCGC 9 cut(s) 432, 537, 542, 599, 729, 734, 778, 1585, 1771
Hin1I GRCGYC 6 cut(s) 267, 447, 597, 732, 776, 873
Hin1II CATG 9 cut(s) 446, 689, 1063, 1695, 1703, 1796, 1859, 2132, 2322
Hin6I GCGC 9 cut(s) 430, 535, 540, 597, 727, 732, 776, 1583, 1769
HinP1I GCGC 9 cut(s) 430, 535, 540, 597, 727, 732, 776, 1583, 1769
HincII GTYRAC 1 cut(s) 1956
HindII GTYRAC 1 cut(s) 1956
HindIII AAGCTT 1 cut(s) 1142
HinfI GANTC 9 cut(s) 46, 137, 234, 317, 387, 603, 623, 1234, 1747
HpaI GTTAAC 1 cut(s) 1956
HphI GGTGA 7 cut(s) 64, 181, 1021, 1030, 1321, 2182, 2233
Hpy166II GTNNAC 5 cut(s) 178, 1594, 1956, 2190, 2290
Hpy188I TCNGA 8 cut(s) 45, 216, 439, 458, 835, 1206, 1455, 2205
Hpy8I GTNNAC 5 cut(s) 178, 1594, 1956, 2190, 2290
Hpy99I CGWCG 7 cut(s) 272, 458, 596, 713, 878, 1049, 1633
HpyAV CCTTC 4 cut(s) 268, 1217, 1291, 1515
HpyCH4III ACNGT 5 cut(s) 709, 995, 1045, 2083, 2095
HpyCH4IV ACGT 4 cut(s) 417, 447, 2103, 2160
HpyF3I CTNAG 5 cut(s) 154, 181, 654, 1833, 1920
HpySE526I ACGT 4 cut(s) 417, 447, 2103, 2160
Hsp92I GRCGYC 6 cut(s) 267, 447, 597, 732, 776, 873
Hsp92II CATG 9 cut(s) 446, 689, 1063, 1695, 1703, 1796, 1859, 2132, 2322
HspAI GCGC 9 cut(s) 430, 535, 540, 597, 727, 732, 776, 1583, 1769
KasI GGCGCC 3 cut(s) 596, 731, 775
KflI GGGWCCC 1 cut(s) 690
Kpn2I TCCGGA 1 cut(s) 1198
KroI GCCGGC 1 cut(s) 778
KroNI GCCGGC 1 cut(s) 780
Ksp22I TGATCA 4 cut(s) 24, 211, 1428, 1852
KspAI GTTAAC 1 cut(s) 1956
KspI CCGCGG 2 cut(s) 527, 2177
Kzo9I GATC 9 cut(s) 24, 211, 789, 1035, 1201, 1335, 1428, 1758, 1852
LmnI GCTCC 5 cut(s) 360, 836, 986, 1366, 1859
Lsp1109I GCAGC 8 cut(s) 21, 284, 1507, 1591, 1702, 1766, 1978, 2257
LweI GCATC 2 cut(s) 1810, 1832
MaeI CTAG 9 cut(s) 50, 305, 1287, 1472, 1488, 1731, 1878, 2006, 2241
MaeII ACGT 4 cut(s) 417, 447, 2103, 2160
MaeIII GTNAC 3 cut(s) 1997, 2095, 2221
MalI GATC 9 cut(s) 26, 213, 791, 1037, 1203, 1337, 1430, 1760, 1854
MboI GATC 9 cut(s) 24, 211, 789, 1035, 1201, 1335, 1428, 1758, 1852
MfeI CAATTG 1 cut(s) 2307
MflI RGATCY 1 cut(s) 1201
MhlI GDGCHC 3 cut(s) 357, 1058, 2292
MluCI AATT 4 cut(s) 1186, 1424, 1619, 2307
Mly113I GGCGCC 3 cut(s) 597, 732, 776
MlyI GAGTC 4 cut(s) 311, 381, 632, 1756
MmeI TCCRAC 4 cut(s) 436, 462, 858, 1387
Mph1103I ATGCAT 1 cut(s) 1825
MreI CGCCGGCG 1 cut(s) 778
MroI TCCGGA 1 cut(s) 1198
MroNI GCCGGC 1 cut(s) 778
MseI TTAA 3 cut(s) 1248, 1445, 1955
MslI CAYNNNNRTG 3 cut(s) 113, 509, 1704
MspA1I CMGCKG 2 cut(s) 526, 2176
MteI GCGCNGCGC 2 cut(s) 538, 730
MunI CAATTG 1 cut(s) 2307
Mva1269I GAATGC 1 cut(s) 1321
MvaI CCWGG 2 cut(s) 677, 1587
MvnI CGCG 8 cut(s) 526, 537, 542, 704, 729, 794, 1769, 2176
NaeI GCCGGC 1 cut(s) 780
NarI GGCGCC 3 cut(s) 597, 732, 776
NciI CCSGG 9 cut(s) 508, 553, 614, 715, 923, 988, 1007, 1895, 1896
NcoI CCATGG 1 cut(s) 1059
NdeII GATC 9 cut(s) 24, 211, 789, 1035, 1201, 1335, 1428, 1758, 1852
NgoMIV GCCGGC 1 cut(s) 778
NlaIII CATG 9 cut(s) 446, 689, 1063, 1695, 1703, 1796, 1859, 2132, 2322
NmeAIII GCCGAG 2 cut(s) 693, 723
NmuCI GTSAC 3 cut(s) 1997, 2095, 2221
NsiI ATGCAT 1 cut(s) 1825
PaeR7I CTCGAG 1 cut(s) 389
PagI TCATGA 1 cut(s) 1792
PceI AGGCCT 1 cut(s) 1504
PcsI WCGNNNNNNNCGW 2 cut(s) 396, 462
PctI GAATGC 1 cut(s) 1321
PdiI GCCGGC 1 cut(s) 780
PfeI GAWTC 5 cut(s) 46, 137, 234, 603, 1234
PflFI GACNNNGTC 2 cut(s) 320, 872
PflMI CCANNNNNTGG 1 cut(s) 420
PleI GAGTC 4 cut(s) 311, 381, 631, 1755
PluTI GGCGCC 3 cut(s) 600, 735, 779
PpsI GAGTC 4 cut(s) 311, 381, 631, 1755
Ppu21I YACGTR 1 cut(s) 2161
PpuMI RGGWCCY 2 cut(s) 690, 2020
Psp124BI GAGCTC 1 cut(s) 357
Psp1406I AACGTT 1 cut(s) 2103
Psp5II RGGWCCY 2 cut(s) 690, 2020
Psp6I CCWGG 2 cut(s) 675, 1585
PspEI GGTNACC 1 cut(s) 2221
PspFI CCCAGC 1 cut(s) 426
PspGI CCWGG 2 cut(s) 675, 1585
PspPI GGNCC 9 cut(s) 423, 690, 717, 998, 1538, 1611, 1625, 2020, 2108
PspPPI RGGWCCY 2 cut(s) 690, 2020
PspXI VCTCGAGB 1 cut(s) 389
PstI CTGCAG 1 cut(s) 2116
PstNI CAGNNNCTG 1 cut(s) 2209
PsuI RGATCY 1 cut(s) 1201
PsyI GACNNNGTC 2 cut(s) 320, 872
RseI CAYNNNNRTG 3 cut(s) 113, 509, 1704
SacI GAGCTC 1 cut(s) 357
SacII CCGCGG 2 cut(s) 527, 2177
SaqAI TTAA 3 cut(s) 1248, 1445, 1955
Sau3AI GATC 9 cut(s) 24, 211, 789, 1035, 1201, 1335, 1428, 1758, 1852
Sau96I GGNCC 9 cut(s) 423, 690, 717, 998, 1538, 1611, 1625, 2020, 2108
ScaI AGTACT 2 cut(s) 186, 462
SchI GAGTC 4 cut(s) 311, 381, 632, 1756
SduI GDGCHC 3 cut(s) 357, 1058, 2292
SfaNI GCATC 2 cut(s) 1810, 1832
SfcI CTRYAG 2 cut(s) 1779, 2112
SfoI GGCGCC 3 cut(s) 598, 733, 777
Sfr274I CTCGAG 1 cut(s) 389
Sfr303I CCGCGG 2 cut(s) 527, 2177
SgrAI CRCCGGYG 1 cut(s) 778
SgrBI CCGCGG 2 cut(s) 527, 2177
SinI GGWCC 6 cut(s) 423, 690, 717, 1611, 1625, 2020
SlaI CTCGAG 1 cut(s) 389
SmaI CCCGGG 1 cut(s) 1896
SmiMI CAYNNNNRTG 3 cut(s) 113, 509, 1704
SmlI CTYRAG 4 cut(s) 389, 1724, 1931, 1970
SmoI CTYRAG 4 cut(s) 389, 1724, 1931, 1970
Sse9I AATT 4 cut(s) 1186, 1424, 1619, 2307
SseBI AGGCCT 1 cut(s) 1504
SspDI GGCGCC 3 cut(s) 596, 731, 775
SspMI CTAG 9 cut(s) 50, 305, 1287, 1472, 1488, 1731, 1878, 2006, 2241
SstI GAGCTC 1 cut(s) 357
StuI AGGCCT 1 cut(s) 1504
StyI CCWWGG 2 cut(s) 1059, 1238
TaaI ACNGT 5 cut(s) 709, 995, 1045, 2083, 2095
TaiI ACGT 4 cut(s) 420, 450, 2106, 2163
TaqI TCGA 6 cut(s) 390, 465, 876, 1190, 1631, 2120
TasI AATT 4 cut(s) 1186, 1424, 1619, 2307
TatI WGTACW 3 cut(s) 184, 460, 657
TfiI GAWTC 5 cut(s) 46, 137, 234, 603, 1234
Tru1I TTAA 3 cut(s) 1248, 1445, 1955
Tru9I TTAA 3 cut(s) 1248, 1445, 1955
TscAI CASTG 3 cut(s) 27, 525, 729
TseFI GTSAC 3 cut(s) 1997, 2095, 2221
TseI GCWGC 8 cut(s) 34, 272, 1520, 1604, 1715, 1754, 1966, 2245
Tsp45I GTSAC 3 cut(s) 1997, 2095, 2221
TspDTI ATGAA 6 cut(s) 17, 1154, 1431, 1580, 1781, 2075
TspGWI ACGGA 3 cut(s) 310, 696, 1617
TspMI CCCGGG 1 cut(s) 1894
TspRI CASTG 3 cut(s) 27, 525, 729
Tth111I GACNNNGTC 2 cut(s) 320, 872
Van91I CCANNNNNTGG 1 cut(s) 420
VneI GTGCAC 1 cut(s) 2288
VpaK11BI GGWCC 6 cut(s) 423, 690, 717, 1611, 1625, 2020
XapI RAATTY 1 cut(s) 1186
XcmI CCANNNNNNNNNTGG 3 cut(s) 27, 683, 1087
XhoI CTCGAG 1 cut(s) 389
XmaI CCCGGG 1 cut(s) 1894
XspI CTAG 9 cut(s) 50, 305, 1287, 1472, 1488, 1731, 1878, 2006, 2241
ZraI GACGTC 1 cut(s) 448
ZrmI AGTACT 2 cut(s) 186, 462
Zsp2I ATGCAT 1 cut(s) 1825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.