Rroxscaffold_4G00282550

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013557
Physical Location & Seq
Reverse (-)
4635689 .. 4640186
4498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282550.1

Sequence Viewer

Length: 2322 bp
ATGAAAATGGTTGAAAAACCAGTGATCAAATGGCTGGTTCTGATTCTAAGCTGCGTCTTCTTCAGCATTGCCCTGTCTGCAAAAACCCAAACTTACATCATCCAAATGGACAAGTCCGCCAAGCCTGAATCTTTCACCACCCACCTCGATTGGTACTCATCCAAAGTAAACTCAGTGCTCAACAAACCAGAAAAAGGAGACGATCAAAACCAAGACAGAATCATTTACACTTACCAAACCGCTTTTCATGGCGTCGCAGCTCAACTGAGCAAACAAGAAGCAGACCTACTAGAGCAACAAGATGGAGTCTTGGCTATATTCCCAGAACAAAAGTACCAGCTCCACACCACCAGAAGTCCTCTGTTTCTCGGGCTCGAACCACACGACAGCACCACCGACAACGTCTGGTCCCAGCGAGTCTCCGACCATGACGTCATCGTAGGAGTACTCGACACTGGGGTCTGGCCGGAGAGCCAGAGCTTCAACGACACCGGAATGTCTCCGGTGCCGCGGCGCTGGAGAGGCGCGTGCGAGACCGGTCGCGGCTTCACAAAAAGCAACTGCAACAGGAAAATCGTCGGCGCCAGAATCTTCCATCGGGGGTACGAGTCCGCCACGGGAAAAATCAACGAGAAGACGGAGTACAAGTCGCCGAGGGACCAGGACGGCCACGGGACCCACACCGCCGCGACCGTCGCCGGATCTCCCGTACGTGGCGCCAATCTTCTCGGCTACGCGTACGGTACGGCAAGAGGAATGGCGCCGGCGGCGAGAATAGCGGTGTACAAGGTGTGCTGGGTCGGAGGTTGTTTCAGCTCTGATATAATGGCGGCAGTTGATAAGGCTGTCGAGGACGGCGTCGATGTTTTGTCTATTTCTCTTGGCGGTGGAGTTTCCTCGTACTACCGGGACAGTTTGTCTATTGCGGCGTTTGGTGCGATGGAGATGGGTGTTTTTGTCTCCTGCTCCGCCGGGAACGGTGGGCCAGACCCGGTGAGTCTGACTAATGTGTCACCTTGGATCACCACCGTCGGTGCTAGCACTATGGATAGGGATTTTCCGGCCACTGTGAAGTTGGGGAATGGGAGGAGTGTAACGGGAGTTTCGCTTTATAGAGGGAGAATGAAGCTTGACACAAAGAAGCTGTACCCAGTTGTGTTAGCGGGTGGGAATTCGAGTAGTCCCAATCCGAGCTCGTTGTGTTTAGAGGGTACTTTAGATCCGAAGAAAGTTAAGGGGAAGATTGTGATTTGTGATCGTGGGATTAGTCCTAGAGTGCAGAAGGGAGACGTGGTGAAGAATGCCGGAGGAGTTGGGATGATCTTGGCGAATACGGCTTCGAATGGGGAGGAGCTTGTTGCTGATTGTCATCTGATTCCGGCGGTTGCGGTGGGAGAGAATGAAGCGAAATCGATCAAGCATTACGCGTTAACAAGTCCGAAAGCAAAGGCAACTTTGGCATTTGTTGGTACTAGAGTTGGGGTTAGACCGTCTCCTATTGTGGCTGCATTTTCGTCAAGAGGGCCTAATCTTTTGAGTTTTGAGATTATGAAGCCGGATTTGGTGGCGCCGGGAGTGAACATTCTTGCTGCTTGGACCGGAGCATTGGGTCCGTCGAGCTTGGCAACGGATGGCAGGAGAGTGAAGTTCAATATCTTGTCCGGGACTTCAATGTCGTGTCCTCATGTGAGTGGGATTGCTGCTTTGCTCAAATCTAGGCACCCTGAATGGAGTCCAGCCGCGATCAAATCCGCTTTGATGACTACAGCTTATGTTCATGACAACACACATAAGCCTCTTGAAGATGCCTCAAAAGCTAAAATTTCAACCCCTTTTGATCATGGAGCTGGACACATAAACCCGAGGAAGGCTCTTGACCCGGGTTTGGTTTACGACATTCAGCCCCAGGATTATCTTGAGTTTCTCTGCACACAAAAGCTAACCCCAACGCAGCTCAAGGTGTTTAGCAATAGCACTTGCCGTCACACTCTGGCCAATGCAGGGGACCTCAACTATCCGGCGATTTCAGTTGTGTTTGTGGAGAACACTAATGTATCTGTCTTGACAGTTCATAGAACTGTCACTAATGTTGGCCCTGCGGTTTCGAGTTACCATGCCATTGTTTCGCCATTCAAGGGTGCTTATGTGAAAGTTGAGCCGAGAATTCTGAAATTCACCAAAGCTAATCAGAAGCTGTCTTACAAAGTGACCTTTACTACAAAGACTAGGCAGACGGAACCTGAGTTTGGAGGTTTGGTGTGGAAGGATGGAGTCCACAGAGTTAGAAGTCCAATTGTTGTGGCAAGGGGGATGCTGATATGA

Protein Analysis

773

Amino Acids

83.21

Weight (kDa)

9.26

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 31 - 115 1.6e-20 Peptidase inhibitor I9
Peptidase_S8 PF00082 143 - 596 1.7e-50 Subtilase family
PA PF02225 383 - 472 1.7e-13 PA domain
fn3_6 PF17766 669 - 767 1.3e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013557)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51750
fragaria_vesca FvH4_7g28501
malus_domestica MD01G1188800.v1.1 MD01G1189100.v1.1 MD01G1189900.v1.1 MD01G1190400.v1.1
prunus_persica Prupe.2G285500_v2.0.a1
pyrus_communis pycom01g20050
rosa_chinensis RchiOBHm_Chr1g0375891
rosa_laevigata RLG00000026646
rosa_multiflora Rmu_sc0001943.1_g000010
rosa_roxburghii Rroxscaffold_4G00282550
rosa_rugosa Rorug01G0392200
rosa_samantha Rh1AG404100 Rh1BG367500 Rh1CG380300 Rh1DG397500
rosa_wichuraiana Rw0G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 431, 458, 1009, 1672
AatII GACGTC 1 cut(s) 435
AccB1I GGYRCC 6 cut(s) 505, 581, 716, 760, 1567, 1719
AccII CGCG 7 cut(s) 511, 527, 543, 689, 737, 1427, 1742
AclWI GGATC 3 cut(s) 709, 1028, 1214
AcoI YGGCCR 4 cut(s) 464, 667, 1062, 1992
AcsI RAATTY 4 cut(s) 1171, 1821, 2163, 2171
AcuI CTGAAG 1 cut(s) 46
AcyI GRCGYC 7 cut(s) 252, 432, 582, 717, 761, 858, 1568
AfiI CCNNNNNNNGG 7 cut(s) 194, 713, 1867, 1885, 2001, 2135, 2246
AflIII ACRYGT 2 cut(s) 735, 1425
AgeI ACCGGT 1 cut(s) 536
AgsI TTSAA 7 cut(s) 14, 484, 1651, 1671, 1802, 1827, 2134
AhdI GACNNNNNGTC 1 cut(s) 916
AjiI CACGTC 1 cut(s) 1291
AjnI CCWGG 2 cut(s) 660, 1905
AjuI GAANNNNNNNTTGG 6 cut(s) 228, 260, 1544, 1576, 2229, 2261
Alw21I GWGCWC 2 cut(s) 180, 1196
Alw26I GTCTC 7 cut(s) 192, 424, 504, 527, 964, 1281, 1497
AlwI GGATC 3 cut(s) 709, 1028, 1214
AlwNI CAGNNNCTG 1 cut(s) 2194
Ama87I CYCGRG 3 cut(s) 368, 1861, 1879
AoxI GGCC 7 cut(s) 464, 667, 983, 1062, 1523, 1992, 2092
ApeKI GCWGC 6 cut(s) 51, 257, 1505, 1589, 1700, 1951
ApoI RAATTY 4 cut(s) 1171, 1821, 2163, 2171
AsiGI ACCGGT 1 cut(s) 536
AspLEI GCGC 6 cut(s) 516, 527, 584, 719, 763, 1570
AspS9I GGNCC 9 cut(s) 408, 658, 675, 983, 1523, 1596, 1610, 2005, 2093
AsuC2I CCSGG 7 cut(s) 908, 973, 992, 1572, 1663, 1880, 1881
AsuHPI GGTGA 6 cut(s) 127, 1005, 1006, 1015, 1306, 2167
AsuII TTCGAA 1 cut(s) 1340
AsuNHI GCTAGC 1 cut(s) 1037
AvaI CYCGRG 3 cut(s) 368, 1861, 1879
AvaII GGWCC 6 cut(s) 408, 658, 675, 1596, 1610, 2005
BaeI ACNNNNGTAYC 6 cut(s) 317, 350, 1461, 1494, 2037, 2070
BalI TGGCCA 1 cut(s) 1994
BanI GGYRCC 6 cut(s) 505, 581, 716, 760, 1567, 1719
BanII GRGCYC 2 cut(s) 375, 1196
BarI GAAGNNNNNNTAC 6 cut(s) 270, 302, 626, 658, 2183, 2215
BbsI GAAGAC 2 cut(s) 49, 641
Bbv12I GWGCWC 2 cut(s) 180, 1196
BbvI GCAGC 6 cut(s) 38, 269, 1492, 1576, 1687, 1963
BccI CCATC 6 cut(s) 296, 603, 934, 940, 1625, 2261
BceAI ACGGC 5 cut(s) 682, 762, 871, 1350, 1965
BciT130I CCWGG 2 cut(s) 662, 1907
BclI TGATCA 2 cut(s) 24, 1837
BcnI CCSGG 7 cut(s) 908, 973, 992, 1572, 1663, 1880, 1881
BcoDI GTCTC 7 cut(s) 192, 424, 504, 527, 964, 1281, 1497
BfaI CTAG 6 cut(s) 290, 1038, 1272, 1473, 1716, 2226
BfmI CTRYAG 1 cut(s) 1764
BfoI RGCGCY 5 cut(s) 517, 585, 720, 764, 1571
BmcAI AGTACT 1 cut(s) 447
Bme1390I CCNGG 9 cut(s) 662, 908, 973, 992, 1572, 1663, 1880, 1881, 1907
Bme18I GGWCC 6 cut(s) 408, 658, 675, 1596, 1610, 2005
BmeRI GACNNNNNGTC 1 cut(s) 916
BmeT110I CYCGRG 3 cut(s) 368, 1861, 1879
BmgBI CACGTC 1 cut(s) 1291
BmgT120I GGNCC 9 cut(s) 408, 658, 675, 983, 1523, 1596, 1610, 2005, 2093
BmrFI CCNGG 9 cut(s) 662, 908, 973, 992, 1572, 1663, 1880, 1881, 1907
BmrI ACTGGG 2 cut(s) 465, 1145
BmsI GCATC 2 cut(s) 1795, 2301
BmtI GCTAGC 1 cut(s) 1041
BmuI ACTGGG 2 cut(s) 465, 1145
BpiI GAAGAC 2 cut(s) 49, 641
BplI GAGNNNNNCTC 2 cut(s) 1855, 1887
BpmI CTGGAG 1 cut(s) 538
Bpu14I TTCGAA 1 cut(s) 1340
BpuEI CTTGAG 2 cut(s) 1937, 1940
BpuMI CCSGG 7 cut(s) 908, 973, 992, 1572, 1663, 1880, 1881
Bsa29I ATCGAT 1 cut(s) 1412
BsaAI YACGTR 1 cut(s) 713
BsaBI GATNNNNATC 1 cut(s) 1368
BsaHI GRCGYC 7 cut(s) 252, 432, 582, 717, 761, 858, 1568
BsaI GGTCTC 1 cut(s) 527
BsaJI CCNNGG 8 cut(s) 509, 615, 653, 670, 1016, 1862, 1879, 1905
BsaWI WCCGGW 4 cut(s) 491, 502, 536, 1598
BsaXI ACNNNNNCTCC 6 cut(s) 632, 662, 1278, 1308, 2241, 2271
Bsc4I CCNNNNNNNGG 7 cut(s) 194, 713, 1867, 1885, 2001, 2135, 2246
Bse118I RCCGGY 2 cut(s) 536, 763
Bse1I ACTGG 3 cut(s) 20, 460, 1151
Bse3DI GCAATG 1 cut(s) 66
Bse8I GATNNNNATC 1 cut(s) 1368
BseBI CCWGG 2 cut(s) 662, 1907
BseCI ATCGAT 1 cut(s) 1412
BseDI CCNNGG 8 cut(s) 509, 615, 653, 670, 1016, 1862, 1879, 1905
BseGI GGATG 6 cut(s) 99, 158, 1323, 1636, 2272, 2316
BseJI GATNNNNATC 1 cut(s) 1368
BseLI CCNNNNNNNGG 7 cut(s) 194, 713, 1867, 1885, 2001, 2135, 2246
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 3 cut(s) 186, 257, 2232
BseNI ACTGG 3 cut(s) 20, 460, 1151
BseRI GAGGAG 3 cut(s) 1102, 1323, 1364
BseXI GCAGC 6 cut(s) 38, 269, 1492, 1576, 1687, 1963
BseYI CCCAGC 2 cut(s) 411, 795
BsgI GTGCAG 2 cut(s) 1298, 1912
Bsh1236I CGCG 7 cut(s) 511, 527, 543, 689, 737, 1427, 1742
Bsh1285I CGRYCG 2 cut(s) 541, 693
BshFI GGCC 7 cut(s) 466, 669, 985, 1064, 1525, 1994, 2094
BshNI GGYRCC 6 cut(s) 505, 581, 716, 760, 1567, 1719
BshTI ACCGGT 1 cut(s) 536
BshVI ATCGAT 1 cut(s) 1412
BsiEI CGRYCG 2 cut(s) 541, 693
BsiHKAI GWGCWC 2 cut(s) 180, 1196
BsiHKCI CYCGRG 3 cut(s) 368, 1861, 1879
BsiWI CGTACG 2 cut(s) 709, 738
BslFI GGGAC 7 cut(s) 394, 671, 688, 923, 1167, 1678, 2018
BslI CCNNNNNNNGG 7 cut(s) 194, 713, 1867, 1885, 2001, 2135, 2246
BsmAI GTCTC 7 cut(s) 192, 424, 504, 527, 964, 1281, 1497
BsmBI CGTCTC 3 cut(s) 192, 1281, 1497
BsmFI GGGAC 7 cut(s) 394, 671, 688, 923, 1167, 1678, 2018
BsmI GAATGC 1 cut(s) 1306
BsnI GGCC 7 cut(s) 466, 669, 985, 1064, 1525, 1994, 2094
Bso31I GGTCTC 1 cut(s) 527
BsoBI CYCGRG 3 cut(s) 368, 1861, 1879
Bsp119I TTCGAA 1 cut(s) 1340
Bsp1286I GDGCHC 3 cut(s) 180, 375, 1196
Bsp1407I TGTACA 1 cut(s) 783
BspANI GGCC 7 cut(s) 466, 669, 985, 1064, 1525, 1994, 2094
BspCNI CTCAG 3 cut(s) 185, 258, 2233
BspDI ATCGAT 1 cut(s) 1412
BspFNI CGCG 7 cut(s) 511, 527, 543, 689, 737, 1427, 1742
BspHI TCATGA 1 cut(s) 1777
BspOI GCTAGC 1 cut(s) 1041
BspPI GGATC 3 cut(s) 709, 1028, 1214
BspT104I TTCGAA 1 cut(s) 1340
BspT107I GGYRCC 6 cut(s) 505, 581, 716, 760, 1567, 1719
BspTNI GGTCTC 1 cut(s) 527
BsrDI GCAATG 1 cut(s) 66
BsrFI RCCGGY 2 cut(s) 536, 763
BsrGI TGTACA 1 cut(s) 783
BsrI ACTGG 3 cut(s) 20, 460, 1151
BssAI RCCGGY 2 cut(s) 536, 763
BssECI CCNNGG 8 cut(s) 509, 615, 653, 670, 1016, 1862, 1879, 1905
BssNI GRCGYC 7 cut(s) 252, 432, 582, 717, 761, 858, 1568
BssT1I CCWWGG 1 cut(s) 1016
Bst2UI CCWGG 2 cut(s) 662, 1907
Bst4CI ACNGT 9 cut(s) 694, 743, 914, 980, 1030, 1069, 1491, 2068, 2080
BstACI GRCGYC 7 cut(s) 252, 432, 582, 717, 761, 858, 1568
BstAUI TGTACA 1 cut(s) 783
BstBAI YACGTR 1 cut(s) 713
BstBI TTCGAA 1 cut(s) 1340
BstC8I GCNNGC 3 cut(s) 529, 765, 1039
BstDEI CTNAG 4 cut(s) 47, 172, 266, 2241
BstDSI CCRYGG 3 cut(s) 509, 615, 670
BstF5I GGATG 6 cut(s) 99, 158, 1323, 1636, 2272, 2316
BstFNI CGCG 7 cut(s) 511, 527, 543, 689, 737, 1427, 1742
BstH2I RGCGCY 5 cut(s) 517, 585, 720, 764, 1571
BstHHI GCGC 6 cut(s) 516, 527, 584, 719, 763, 1570
BstMAI GTCTC 7 cut(s) 192, 424, 504, 527, 964, 1281, 1497
BstMCI CGRYCG 2 cut(s) 541, 693
BstMWI GCNNNNNNNGC 9 cut(s) 77, 522, 695, 767, 776, 935, 1334, 1457, 1814
BstNI CCWGG 2 cut(s) 662, 1907
BstSCI CCNGG 9 cut(s) 660, 906, 971, 990, 1570, 1661, 1878, 1879, 1905
BstSFI CTRYAG 1 cut(s) 1764
BstUI CGCG 7 cut(s) 511, 527, 543, 689, 737, 1427, 1742
BstV1I GCAGC 6 cut(s) 38, 269, 1492, 1576, 1687, 1963
BstV2I GAAGAC 2 cut(s) 49, 641
BstX2I RGATCY 2 cut(s) 701, 1219
BstYI RGATCY 2 cut(s) 701, 1219
Bsu15I ATCGAT 1 cut(s) 1412
BsuRI GGCC 7 cut(s) 466, 669, 985, 1064, 1525, 1994, 2094
BsuTUI ATCGAT 1 cut(s) 1412
BtgI CCRYGG 3 cut(s) 509, 615, 670
BtgZI GCGATG 1 cut(s) 953
BtrI CACGTC 1 cut(s) 1291
BtsCI GGATG 6 cut(s) 99, 158, 1323, 1636, 2272, 2316
BtsIMutI CAGTG 4 cut(s) 27, 180, 453, 1065
Cac8I GCNNGC 3 cut(s) 529, 765, 1039
CaiI CAGNNNCTG 1 cut(s) 2194
CciI TCATGA 1 cut(s) 1777
CfoI GCGC 6 cut(s) 516, 527, 584, 719, 763, 1570
Cfr10I RCCGGY 2 cut(s) 536, 763
Cfr13I GGNCC 9 cut(s) 408, 658, 675, 983, 1523, 1596, 1610, 2005, 2093
Cfr42I CCGCGG 1 cut(s) 512
Cfr9I CCCGGG 1 cut(s) 1879
ClaI ATCGAT 1 cut(s) 1412
CseI GACGC 3 cut(s) 43, 241, 847
CspAI ACCGGT 1 cut(s) 536
CspCI CAANNNNNGTGG 4 cut(s) 131, 166, 2280, 2315
CviAII CATG 6 cut(s) 248, 428, 1685, 1778, 1841, 2114
DdeI CTNAG 4 cut(s) 47, 172, 266, 2241
DinI GGCGCC 4 cut(s) 583, 718, 762, 1569
DrdI GACNNNNNNGTC 4 cut(s) 431, 458, 1009, 1672
DriI GACNNNNNGTC 1 cut(s) 916
DseDI GACNNNNNNGTC 4 cut(s) 431, 458, 1009, 1672
EaeI YGGCCR 4 cut(s) 464, 667, 1062, 1992
Eam1105I GACNNNNNGTC 1 cut(s) 916
EciI GGCGGA 3 cut(s) 106, 601, 958
Ecl136II GAGCTC 1 cut(s) 1194
Eco130I CCWWGG 1 cut(s) 1016
Eco24I GRGCYC 2 cut(s) 375, 1196
Eco31I GGTCTC 1 cut(s) 527
Eco47I GGWCC 6 cut(s) 408, 658, 675, 1596, 1610, 2005
Eco53kI GAGCTC 1 cut(s) 1194
Eco57I CTGAAG 1 cut(s) 46
Eco88I CYCGRG 3 cut(s) 368, 1861, 1879
EcoICRI GAGCTC 1 cut(s) 1194
EcoO109I RGGNCCY 3 cut(s) 675, 1523, 2005
EcoRI GAATTC 2 cut(s) 1171, 2163
EcoRII CCWGG 2 cut(s) 660, 1905
EcoT14I CCWWGG 1 cut(s) 1016
EcoT38I GRGCYC 2 cut(s) 375, 1196
EgeI GGCGCC 4 cut(s) 583, 718, 762, 1569
EheI GGCGCC 4 cut(s) 583, 718, 762, 1569
ErhI CCWWGG 1 cut(s) 1016
Esp3I CGTCTC 3 cut(s) 192, 1281, 1497
FaeI CATG 6 cut(s) 251, 431, 1688, 1781, 1844, 2117
FaqI GGGAC 7 cut(s) 394, 671, 688, 923, 1167, 1678, 2018
FatI CATG 6 cut(s) 247, 427, 1684, 1777, 1840, 2113
FauI CCCGC 1 cut(s) 1156
FbaI TGATCA 2 cut(s) 24, 1837
FokI GGATG 5 cut(s) 86, 145, 1330, 1643, 2279
FriOI GRGCYC 2 cut(s) 375, 1196
FspBI CTAG 6 cut(s) 290, 1038, 1272, 1473, 1716, 2226
GlaI GCGC 6 cut(s) 515, 526, 583, 718, 762, 1569
GsaI CCCAGC 2 cut(s) 415, 799
GsuI CTGGAG 1 cut(s) 538
HaeII RGCGCY 5 cut(s) 517, 585, 720, 764, 1571
HaeIII GGCC 7 cut(s) 466, 669, 985, 1064, 1525, 1994, 2094
HgaI GACGC 3 cut(s) 43, 241, 847
HhaI GCGC 6 cut(s) 516, 527, 584, 719, 763, 1570
Hin1I GRCGYC 7 cut(s) 252, 432, 582, 717, 761, 858, 1568
Hin1II CATG 6 cut(s) 251, 431, 1688, 1781, 1844, 2117
Hin6I GCGC 6 cut(s) 514, 525, 582, 717, 761, 1568
HinP1I GCGC 6 cut(s) 514, 525, 582, 717, 761, 1568
HincII GTYRAC 1 cut(s) 1431
HindII GTYRAC 1 cut(s) 1431
HindIII AAGCTT 1 cut(s) 1127
HpaI GTTAAC 1 cut(s) 1431
HphI GGTGA 6 cut(s) 127, 1005, 1006, 1015, 1306, 2167
Hpy166II GTNNAC 6 cut(s) 169, 784, 1431, 1579, 1891, 2275
Hpy188III TCNNGA 6 cut(s) 1518, 1778, 1799, 1874, 1916, 2062
Hpy8I GTNNAC 6 cut(s) 169, 784, 1431, 1579, 1891, 2275
Hpy99I CGWCG 6 cut(s) 257, 581, 698, 863, 1034, 1618
HpyAV CCTTC 3 cut(s) 1276, 1861, 2257
HpyCH4III ACNGT 9 cut(s) 694, 743, 914, 980, 1030, 1069, 1491, 2068, 2080
HpyCH4IV ACGT 4 cut(s) 402, 432, 712, 1290
HpyCH4V TGCA 6 cut(s) 80, 564, 1279, 1508, 1929, 2000
HpyF10VI GCNNNNNNNGC 9 cut(s) 77, 522, 695, 767, 776, 935, 1334, 1457, 1814
HpyF3I CTNAG 4 cut(s) 47, 172, 266, 2241
HpySE526I ACGT 4 cut(s) 402, 432, 712, 1290
Hsp92I GRCGYC 7 cut(s) 252, 432, 582, 717, 761, 858, 1568
Hsp92II CATG 6 cut(s) 251, 431, 1688, 1781, 1844, 2117
HspAI GCGC 6 cut(s) 514, 525, 582, 717, 761, 1568
KasI GGCGCC 4 cut(s) 581, 716, 760, 1567
KflI GGGWCCC 1 cut(s) 675
KroI GCCGGC 1 cut(s) 763
KroNI GCCGGC 1 cut(s) 765
Ksp22I TGATCA 2 cut(s) 24, 1837
KspAI GTTAAC 1 cut(s) 1431
KspI CCGCGG 1 cut(s) 512
LmnI GCTCC 5 cut(s) 345, 971, 1351, 1601, 1844
Lsp1109I GCAGC 6 cut(s) 38, 269, 1492, 1576, 1687, 1963
LweI GCATC 2 cut(s) 1795, 2301
MaeI CTAG 6 cut(s) 290, 1038, 1272, 1473, 1716, 2226
MaeII ACGT 4 cut(s) 402, 432, 712, 1290
MaeIII GTNAC 6 cut(s) 1011, 1093, 1982, 2080, 2108, 2206
MboII GAAGA 9 cut(s) 49, 52, 583, 646, 716, 1237, 1252, 1309, 1814
MfeI CAATTG 1 cut(s) 2292
MflI RGATCY 2 cut(s) 701, 1219
MhlI GDGCHC 3 cut(s) 180, 375, 1196
MlsI TGGCCA 1 cut(s) 1994
MluCI AATT 5 cut(s) 1171, 1821, 2163, 2171, 2292
MluI ACGCGT 2 cut(s) 735, 1425
MluNI TGGCCA 1 cut(s) 1994
Mly113I GGCGCC 4 cut(s) 582, 717, 761, 1568
MlyI GAGTC 6 cut(s) 315, 426, 617, 1006, 1741, 2280
MmeI TCCRAC 2 cut(s) 447, 781
Mox20I TGGCCA 1 cut(s) 1994
MreI CGCCGGCG 1 cut(s) 763
MroNI GCCGGC 1 cut(s) 763
MscI TGGCCA 1 cut(s) 1994
MseI TTAA 2 cut(s) 1233, 1430
MslI CAYNNNNRTG 3 cut(s) 104, 494, 1689
Msp20I TGGCCA 1 cut(s) 1994
MspA1I CMGCKG 1 cut(s) 511
MspR9I CCNGG 9 cut(s) 662, 908, 973, 992, 1572, 1663, 1880, 1881, 1907
MunI CAATTG 1 cut(s) 2292
Mva1269I GAATGC 1 cut(s) 1306
MvaI CCWGG 2 cut(s) 662, 1907
MvnI CGCG 7 cut(s) 511, 527, 543, 689, 737, 1427, 1742
MwoI GCNNNNNNNGC 9 cut(s) 77, 522, 695, 767, 776, 935, 1334, 1457, 1814
NaeI GCCGGC 1 cut(s) 765
NarI GGCGCC 4 cut(s) 582, 717, 761, 1568
NciI CCSGG 7 cut(s) 908, 973, 992, 1572, 1663, 1880, 1881
NgoMIV GCCGGC 1 cut(s) 763
NheI GCTAGC 1 cut(s) 1037
NlaIII CATG 6 cut(s) 251, 431, 1688, 1781, 1844, 2117
NmeAIII GCCGAG 3 cut(s) 678, 708, 2184
NmuCI GTSAC 4 cut(s) 1011, 1982, 2080, 2206
NspV TTCGAA 1 cut(s) 1340
PagI TCATGA 1 cut(s) 1777
PcsI WCGNNNNNNNCGW 2 cut(s) 381, 855
PctI GAATGC 1 cut(s) 1306
PdiI GCCGGC 1 cut(s) 765
PfeI GAWTC 5 cut(s) 43, 128, 219, 588, 1375
Pfl23II CGTACG 2 cut(s) 709, 738
PflFI GACNNNGTC 2 cut(s) 401, 857
PfoI TCCNGGA 1 cut(s) 1661
PinAI ACCGGT 1 cut(s) 536
PleI GAGTC 6 cut(s) 314, 425, 616, 1005, 1740, 2279
PluTI GGCGCC 4 cut(s) 585, 720, 764, 1571
PpsI GAGTC 6 cut(s) 314, 425, 616, 1005, 1740, 2279
Ppu21I YACGTR 1 cut(s) 713
PpuMI RGGWCCY 2 cut(s) 675, 2005
Psp124BI GAGCTC 1 cut(s) 1196
Psp5II RGGWCCY 2 cut(s) 675, 2005
Psp6I CCWGG 2 cut(s) 660, 1905
PspFI CCCAGC 2 cut(s) 411, 795
PspGI CCWGG 2 cut(s) 660, 1905
PspLI CGTACG 2 cut(s) 709, 738
PspPI GGNCC 9 cut(s) 408, 658, 675, 983, 1523, 1596, 1610, 2005, 2093
PspPPI RGGWCCY 2 cut(s) 675, 2005
PstNI CAGNNNCTG 1 cut(s) 2194
PsuI RGATCY 2 cut(s) 701, 1219
PsyI GACNNNGTC 2 cut(s) 401, 857
RseI CAYNNNNRTG 3 cut(s) 104, 494, 1689
SacI GAGCTC 1 cut(s) 1196
SacII CCGCGG 1 cut(s) 512
SaqAI TTAA 2 cut(s) 1233, 1430
Sau96I GGNCC 9 cut(s) 408, 658, 675, 983, 1523, 1596, 1610, 2005, 2093
ScaI AGTACT 1 cut(s) 447
SchI GAGTC 6 cut(s) 315, 426, 617, 1006, 1741, 2280
ScrFI CCNGG 9 cut(s) 662, 908, 973, 992, 1572, 1663, 1880, 1881, 1907
SduI GDGCHC 3 cut(s) 180, 375, 1196
SfaNI GCATC 2 cut(s) 1795, 2301
SfcI CTRYAG 1 cut(s) 1764
SfoI GGCGCC 4 cut(s) 583, 718, 762, 1569
Sfr303I CCGCGG 1 cut(s) 512
SfuI TTCGAA 1 cut(s) 1340
SgrAI CRCCGGYG 1 cut(s) 763
SgrBI CCGCGG 1 cut(s) 512
SinI GGWCC 6 cut(s) 408, 658, 675, 1596, 1610, 2005
SmaI CCCGGG 1 cut(s) 1881
SmiMI CAYNNNNRTG 3 cut(s) 104, 494, 1689
SmlI CTYRAG 2 cut(s) 1916, 1955
SmoI CTYRAG 2 cut(s) 1916, 1955
Sse9I AATT 5 cut(s) 1171, 1821, 2163, 2171, 2292
SspDI GGCGCC 4 cut(s) 581, 716, 760, 1567
SspMI CTAG 6 cut(s) 290, 1038, 1272, 1473, 1716, 2226
SstI GAGCTC 1 cut(s) 1196
StyD4I CCNGG 9 cut(s) 660, 906, 971, 990, 1570, 1661, 1878, 1879, 1905
StyI CCWWGG 1 cut(s) 1016
TaaI ACNGT 9 cut(s) 694, 743, 914, 980, 1030, 1069, 1491, 2068, 2080
TaiI ACGT 4 cut(s) 405, 435, 715, 1293
TasI AATT 5 cut(s) 1171, 1821, 2163, 2171, 2292
TatI WGTACW 3 cut(s) 445, 642, 783
TauI GCSGC 8 cut(s) 511, 514, 546, 689, 770, 833, 929, 1742
TfiI GAWTC 5 cut(s) 43, 128, 219, 588, 1375
Tru1I TTAA 2 cut(s) 1233, 1430
Tru9I TTAA 2 cut(s) 1233, 1430
TscAI CASTG 4 cut(s) 27, 180, 460, 1072
TseFI GTSAC 4 cut(s) 1011, 1982, 2080, 2206
TseI GCWGC 6 cut(s) 51, 257, 1505, 1589, 1700, 1951
Tsp45I GTSAC 4 cut(s) 1011, 1982, 2080, 2206
TspDTI ATGAA 7 cut(s) 17, 236, 1139, 1416, 1565, 1766, 2060
TspGWI ACGGA 4 cut(s) 653, 1602, 1643, 2249
TspMI CCCGGG 1 cut(s) 1879
TspRI CASTG 4 cut(s) 27, 180, 460, 1072
Tth111I GACNNNGTC 2 cut(s) 401, 857
VpaK11BI GGWCC 6 cut(s) 408, 658, 675, 1596, 1610, 2005
XapI RAATTY 4 cut(s) 1171, 1821, 2163, 2171
XcmI CCANNNNNNNNNTGG 2 cut(s) 27, 1072
XmaI CCCGGG 1 cut(s) 1879
XspI CTAG 6 cut(s) 290, 1038, 1272, 1473, 1716, 2226
ZraI GACGTC 1 cut(s) 433
ZrmI AGTACT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.