RLG00000026646

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013557
Physical Location & Seq
Reverse (-)
4574661 .. 4576976
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026646

Sequence Viewer

Length: 2316 bp
ATGGTTGAAAAACCAGTGATCAAATGGCTGGTTCTGATTCTAAGCTGCGTCTTCTTCAGCATTGCCCTCCCTGCAAAAACCCAAACTTACATCATCCAAATGGACAAGTCCGCCAAGCCTGAATCTTTCACCACCCACCTCGATTGGTACTCATCCAAAATAAACTCAGTACTCAACAAACCAGGAAAAGGAGACGATCAAAACCAAGACAGAATCATTTACACTTATCAAACCGCCTTTCATGGCGTCGCAGCTCAGCTGAGCAAACAAGAAGCGGACCTACTAGAGCAACAAGATGGAGTCTTGGCTATATTCCCAGAACAAAAGTACCAGCTCCACACCACCAGAAGTCCTCTGTTTCTCGGGCTCGAACCACACGACAGCACCACCGACAACGTCTGGTCCCAGAGAGTCTCCGACCATGACGTCATCGTCGGAGTACTCGACACGGGGGTCTGGCCGGAGAGCCAGAGCTTCAACGACACCGGAATGTCTCCGGTGCCGCGGCGCTGGAGAGGCGCGTGCGAGACCGGCCGCGGCTTCACAAAAAGCAACTGCAACAGGAAAATCGTCGGCGCCAGAATCTTCCACCGGGGGTACGAGTCCGCCACCGGAAAAATCAACGAGAAGACGGAGTACAAGTCGCCGAGGGACCAGGACGGACACGGGACTCACACCGCCGCAACCGTCGCCGGATCTCCCGTACGTGGCGCCAATCTTCTCGGCTACGCGTACGGTACGGCAAGAGGAATGGCGCCGGCGGCGAGGATCGCAGCGTACAAGGTGTGCTGGGTCGGAGGTTGTTTCAGCTCTGATATAATGGCGGCGGTTGATAAGGCCGTCGAGGACGGCGTCGATGTTTTGTCTATTTCTCTTGGCGGTGGAGTTTCCTCGTATTACCGGGATAGCTTGTCTATTGCGGCGTTTGGGGCGATGGAGATGGGTGTTTTTGTCTCCTGCTCCGCCGGGAACGGTGGGCCAGACCCGGTGAGTCTGACTAATGTGTCACCTTGGATCACCACCGTCGGTGCTAGCACTATGGATAGGGATTTTCCGGCCACTGTGAAGTTGGGGAATGGGAGGACTATCACTGGAGTTTCGCTTTATAGAGGGAGAATGAAGCTTGACGCAAAGAAGCTGTACCCTGTTGTGTTAGCGGGTGGGAATTCGAGCAGTCCCAATCCGAGCTCGTTGTGTTTAGAGGGTACTTTGGATCCGAGGAAAGTTAAGGGGAAGATTGTGATTTGTGATCGTGGGATTAGTCCTAGAGTGCAGAAGGGAGACGTGGTGAAGAATGCCGGAGGAGTTGGGATGATCTTGGCGAATACGGCTTCCAATGGGGAGGAGCTTGTTGCTGATTGTCATCTGATTCCGGCGGTTGCGGTGGGAGAGAATGAAGCAAAATCGATCAAGCATTACGCTCTAACAAGTCCGAAAGCAAAGGCAACTTTAGCATTTGTTGGTACTAGAGTTGGGGTTAGACCGTCTCCCATTGTGGCTGCATTTTCATCAAGAGGGCCTAATCTTTTGAGTTTTGAGATTATGAAGCCGGATTTGGTGGCGCCGGGAGTGAACATTCTTGCTGCTTGGACCGGAGCATTGGGTCCGTCGAGCTTGGCAACGGATAGCAGGAGAGTGAAGTTCAATATCTTGTCCGGGACTTCAATGTCATGTCCTCATGTGAGTGGGATTGCTGCTTTGCTCAAATCTAGGCACCCCGAATGGAGTCCAGCCGAGATCAAATCCGCCTTGATGACTACAGCTTATGTTCATGACAACACACATAAGCCTCTGGAAGATGCCTCAGAAGCTAAAATTTCAACCCCTTTTGATCATGGAGCTGGACACATAAACCCGAGGAAGGCTCTTGACCCGGGCTTGGTTTATGACATTCAGCCCCAGGATTATCTTGAGTTCCTCTGCACACAAAAGCTAACCCCAACGCAGCTCAAGGTGTTTAGCAATAGCACTTGCCGTCACACTCTGGCCAATGCAGGGGACCTCAACTATCCGGCAATTTCAGTTGTGTTTGTGGAGAACACTAATGTCTCTGTCTTGACAGTTCATAGAACTGTCACTAATGTTGGCCCTGCGCTTTCGAGTTACCATGCCATAGTTTCGCCATTCAAGGGTGCTTATGTGAAAGTTGAGCCGCGAATTCTGAAATTCACCAAAGCTAATCAGAAGCTGTCTTACAAAGTGACCTTTACTACAAAGACTAGGCAGACGGAACCTGAGTTTGGAGGCTTGGTGTGGAAGGATGGAGTGCACAGAGTTAGAAGTCCAATTGTTGTGGCATGGGGGATGCTGATATGA

Protein Analysis

772

Amino Acids

83.04

Weight (kDa)

9.13

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 29 - 113 2e-20 Peptidase inhibitor I9
Peptidase_S8 PF00082 141 - 596 1.1e-51 Subtilase family
PA PF02225 381 - 470 1.8e-13 PA domain
fn3_6 PF17766 667 - 765 1.5e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013557)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51750
fragaria_vesca FvH4_7g28501
malus_domestica MD01G1188800.v1.1 MD01G1189100.v1.1 MD01G1189900.v1.1 MD01G1190400.v1.1
prunus_persica Prupe.2G285500_v2.0.a1
pyrus_communis pycom01g20050
rosa_chinensis RchiOBHm_Chr1g0375891
rosa_laevigata RLG00000026646
rosa_multiflora Rmu_sc0001943.1_g000010
rosa_roxburghii Rroxscaffold_4G00282550
rosa_rugosa Rorug01G0392200
rosa_samantha Rh1AG404100 Rh1BG367500 Rh1CG380300 Rh1DG397500
rosa_wichuraiana Rw0G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 5 cut(s) 425, 431, 452, 1003, 1666
AatII GACGTC 1 cut(s) 429
AccB1I GGYRCC 6 cut(s) 499, 575, 710, 754, 1561, 1713
AccII CGCG 5 cut(s) 505, 521, 537, 731, 2155
AclWI GGATC 5 cut(s) 703, 776, 1022, 1208, 1221
AcoI YGGCCR 4 cut(s) 458, 532, 1056, 1986
AcsI RAATTY 4 cut(s) 1165, 1815, 2157, 2165
AcuI CTGAAG 1 cut(s) 40
AcyI GRCGYC 7 cut(s) 246, 426, 576, 711, 755, 852, 1562
AfiI CCNNNNNNNGG 7 cut(s) 188, 707, 1861, 1879, 1995, 2129, 2240
AflIII ACRYGT 1 cut(s) 729
AgsI TTSAA 6 cut(s) 8, 478, 1645, 1665, 1821, 2128
AjiI CACGTC 1 cut(s) 1285
AjnI CCWGG 3 cut(s) 181, 654, 1899
AjuI GAANNNNNNNTTGG 4 cut(s) 1538, 1570, 2223, 2255
Alw21I GWGCWC 2 cut(s) 1190, 2271
Alw26I GTCTC 8 cut(s) 186, 418, 498, 521, 958, 1275, 1491, 2053
Alw44I GTGCAC 1 cut(s) 2267
AlwI GGATC 5 cut(s) 703, 776, 1022, 1208, 1221
AlwNI CAGNNNCTG 1 cut(s) 2188
Ama87I CYCGRG 3 cut(s) 362, 1855, 1873
AoxI GGCC 8 cut(s) 458, 532, 837, 977, 1056, 1517, 1986, 2086
ApaLI GTGCAC 1 cut(s) 2267
ApeKI GCWGC 7 cut(s) 45, 251, 773, 1499, 1583, 1694, 1945
ApoI RAATTY 4 cut(s) 1165, 1815, 2157, 2165
AspLEI GCGC 7 cut(s) 510, 521, 578, 713, 757, 1564, 2095
AspS9I GGNCC 9 cut(s) 277, 402, 652, 977, 1517, 1590, 1604, 1999, 2087
AsuC2I CCSGG 8 cut(s) 593, 902, 967, 986, 1566, 1657, 1874, 1875
AsuHPI GGTGA 6 cut(s) 121, 999, 1000, 1009, 1300, 2161
AsuNHI GCTAGC 1 cut(s) 1031
AvaI CYCGRG 3 cut(s) 362, 1855, 1873
AvaII GGWCC 6 cut(s) 277, 402, 652, 1590, 1604, 1999
BaeGI GKGCMC 1 cut(s) 2271
BaeI ACNNNNGTAYC 4 cut(s) 311, 344, 1455, 1488
BalI TGGCCA 1 cut(s) 1988
BamHI GGATCC 1 cut(s) 1213
BanI GGYRCC 6 cut(s) 499, 575, 710, 754, 1561, 1713
BanII GRGCYC 2 cut(s) 369, 1190
BarI GAAGNNNNNNTAC 6 cut(s) 264, 296, 620, 652, 2177, 2209
BbsI GAAGAC 2 cut(s) 43, 635
Bbv12I GWGCWC 2 cut(s) 1190, 2271
BbvI GCAGC 7 cut(s) 32, 263, 785, 1486, 1570, 1681, 1957
BccI CCATC 4 cut(s) 290, 928, 934, 2255
BceAI ACGGC 5 cut(s) 756, 824, 865, 1344, 1959
BciT130I CCWGG 3 cut(s) 183, 656, 1901
BclI TGATCA 2 cut(s) 18, 1831
BcnI CCSGG 8 cut(s) 593, 902, 967, 986, 1566, 1657, 1874, 1875
BcoDI GTCTC 8 cut(s) 186, 418, 498, 521, 958, 1275, 1491, 2053
BfaI CTAG 6 cut(s) 284, 1032, 1266, 1467, 1710, 2220
BfmI CTRYAG 1 cut(s) 1758
BfoI RGCGCY 5 cut(s) 511, 579, 714, 758, 1565
BlpI GCTNAGC 2 cut(s) 255, 260
BmcAI AGTACT 2 cut(s) 171, 441
Bme18I GGWCC 6 cut(s) 277, 402, 652, 1590, 1604, 1999
BmeT110I CYCGRG 3 cut(s) 362, 1855, 1873
BmgBI CACGTC 1 cut(s) 1285
BmgT120I GGNCC 9 cut(s) 277, 402, 652, 977, 1517, 1590, 1604, 1999, 2087
BmsI GCATC 2 cut(s) 1789, 2295
BmtI GCTAGC 1 cut(s) 1035
BpiI GAAGAC 2 cut(s) 43, 635
BplI GAGNNNNNCTC 2 cut(s) 1849, 1881
BpmI CTGGAG 2 cut(s) 532, 1113
Bpu1102I GCTNAGC 2 cut(s) 255, 260
BpuEI CTTGAG 2 cut(s) 1931, 1934
BpuMI CCSGG 8 cut(s) 593, 902, 967, 986, 1566, 1657, 1874, 1875
Bsa29I ATCGAT 1 cut(s) 1406
BsaAI YACGTR 1 cut(s) 707
BsaBI GATNNNNATC 1 cut(s) 1362
BsaHI GRCGYC 7 cut(s) 246, 426, 576, 711, 755, 852, 1562
BsaI GGTCTC 1 cut(s) 521
BsaJI CCNNGG 9 cut(s) 503, 535, 592, 647, 1010, 1217, 1856, 1873, 1899
BsaWI WCCGGW 4 cut(s) 485, 496, 611, 1592
BsaXI ACNNNNNCTCC 6 cut(s) 626, 656, 1272, 1302, 2235, 2265
Bsc4I CCNNNNNNNGG 7 cut(s) 188, 707, 1861, 1879, 1995, 2129, 2240
Bse118I RCCGGY 2 cut(s) 530, 757
Bse1I ACTGG 2 cut(s) 14, 1096
Bse3DI GCAATG 1 cut(s) 60
Bse8I GATNNNNATC 1 cut(s) 1362
BseBI CCWGG 3 cut(s) 183, 656, 1901
BseCI ATCGAT 1 cut(s) 1406
BseDI CCNNGG 9 cut(s) 503, 535, 592, 647, 1010, 1217, 1856, 1873, 1899
BseGI GGATG 5 cut(s) 93, 152, 1317, 2266, 2310
BseJI GATNNNNATC 1 cut(s) 1362
BseLI CCNNNNNNNGG 7 cut(s) 188, 707, 1861, 1879, 1995, 2129, 2240
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 5 cut(s) 180, 251, 269, 1818, 2226
BseNI ACTGG 2 cut(s) 14, 1096
BseRI GAGGAG 2 cut(s) 1317, 1358
BseSI GKGCMC 1 cut(s) 2271
BseX3I CGGCCG 1 cut(s) 532
BseXI GCAGC 7 cut(s) 32, 263, 785, 1486, 1570, 1681, 1957
BseYI CCCAGC 1 cut(s) 789
BsgI GTGCAG 2 cut(s) 1292, 1906
Bsh1236I CGCG 5 cut(s) 505, 521, 537, 731, 2155
Bsh1285I CGRYCG 1 cut(s) 535
BshFI GGCC 8 cut(s) 460, 534, 839, 979, 1058, 1519, 1988, 2088
BshNI GGYRCC 6 cut(s) 499, 575, 710, 754, 1561, 1713
BshVI ATCGAT 1 cut(s) 1406
BsiEI CGRYCG 1 cut(s) 535
BsiHKAI GWGCWC 2 cut(s) 1190, 2271
BsiHKCI CYCGRG 3 cut(s) 362, 1855, 1873
BsiWI CGTACG 2 cut(s) 703, 732
BslFI GGGAC 6 cut(s) 388, 665, 682, 1161, 1672, 2012
BslI CCNNNNNNNGG 7 cut(s) 188, 707, 1861, 1879, 1995, 2129, 2240
BsmAI GTCTC 8 cut(s) 186, 418, 498, 521, 958, 1275, 1491, 2053
BsmBI CGTCTC 3 cut(s) 186, 1275, 1491
BsmFI GGGAC 6 cut(s) 388, 665, 682, 1161, 1672, 2012
BsmI GAATGC 1 cut(s) 1300
BsnI GGCC 8 cut(s) 460, 534, 839, 979, 1058, 1519, 1988, 2088
Bso31I GGTCTC 1 cut(s) 521
BsoBI CYCGRG 3 cut(s) 362, 1855, 1873
Bsp1286I GDGCHC 3 cut(s) 369, 1190, 2271
Bsp1720I GCTNAGC 2 cut(s) 255, 260
BspANI GGCC 8 cut(s) 460, 534, 839, 979, 1058, 1519, 1988, 2088
BspCNI CTCAG 5 cut(s) 179, 252, 268, 1817, 2227
BspDI ATCGAT 1 cut(s) 1406
BspFNI CGCG 5 cut(s) 505, 521, 537, 731, 2155
BspHI TCATGA 1 cut(s) 1771
BspOI GCTAGC 1 cut(s) 1035
BspPI GGATC 5 cut(s) 703, 776, 1022, 1208, 1221
BspT107I GGYRCC 6 cut(s) 499, 575, 710, 754, 1561, 1713
BspTNI GGTCTC 1 cut(s) 521
BsrDI GCAATG 1 cut(s) 60
BsrFI RCCGGY 2 cut(s) 530, 757
BsrI ACTGG 2 cut(s) 14, 1096
BssAI RCCGGY 2 cut(s) 530, 757
BssECI CCNNGG 9 cut(s) 503, 535, 592, 647, 1010, 1217, 1856, 1873, 1899
BssNI GRCGYC 7 cut(s) 246, 426, 576, 711, 755, 852, 1562
BssT1I CCWWGG 1 cut(s) 1010
Bst2UI CCWGG 3 cut(s) 183, 656, 1901
Bst4CI ACNGT 8 cut(s) 688, 737, 974, 1024, 1063, 1485, 2062, 2074
BstACI GRCGYC 7 cut(s) 246, 426, 576, 711, 755, 852, 1562
BstBAI YACGTR 1 cut(s) 707
BstC8I GCNNGC 3 cut(s) 523, 759, 1033
BstDEI CTNAG 6 cut(s) 41, 166, 255, 260, 1804, 2235
BstDSI CCRYGG 2 cut(s) 503, 535
BstF5I GGATG 5 cut(s) 93, 152, 1317, 2266, 2310
BstFNI CGCG 5 cut(s) 505, 521, 537, 731, 2155
BstH2I RGCGCY 5 cut(s) 511, 579, 714, 758, 1565
BstHHI GCGC 7 cut(s) 510, 521, 578, 713, 757, 1564, 2095
BstMAI GTCTC 8 cut(s) 186, 418, 498, 521, 958, 1275, 1491, 2053
BstMCI CGRYCG 1 cut(s) 535
BstNI CCWGG 3 cut(s) 183, 656, 1901
BstSFI CTRYAG 1 cut(s) 1758
BstSLI GKGCMC 1 cut(s) 2271
BstUI CGCG 5 cut(s) 505, 521, 537, 731, 2155
BstV1I GCAGC 7 cut(s) 32, 263, 785, 1486, 1570, 1681, 1957
BstV2I GAAGAC 2 cut(s) 43, 635
BstX2I RGATCY 2 cut(s) 695, 1213
BstYI RGATCY 2 cut(s) 695, 1213
BstZI CGGCCG 1 cut(s) 532
Bsu15I ATCGAT 1 cut(s) 1406
BsuRI GGCC 8 cut(s) 460, 534, 839, 979, 1058, 1519, 1988, 2088
BsuTUI ATCGAT 1 cut(s) 1406
BtgI CCRYGG 2 cut(s) 503, 535
BtgZI GCGATG 1 cut(s) 947
BtrI CACGTC 1 cut(s) 1285
BtsCI GGATG 5 cut(s) 93, 152, 1317, 2266, 2310
BtsIMutI CAGTG 3 cut(s) 21, 1059, 1089
Cac8I GCNNGC 3 cut(s) 523, 759, 1033
CaiI CAGNNNCTG 1 cut(s) 2188
CciI TCATGA 1 cut(s) 1771
CfoI GCGC 7 cut(s) 510, 521, 578, 713, 757, 1564, 2095
Cfr10I RCCGGY 2 cut(s) 530, 757
Cfr13I GGNCC 9 cut(s) 277, 402, 652, 977, 1517, 1590, 1604, 1999, 2087
Cfr42I CCGCGG 2 cut(s) 506, 538
Cfr9I CCCGGG 1 cut(s) 1873
ClaI ATCGAT 1 cut(s) 1406
CseI GACGC 4 cut(s) 37, 235, 841, 1136
CspCI CAANNNNNGTGG 4 cut(s) 125, 160, 2274, 2309
CviAII CATG 8 cut(s) 242, 422, 1671, 1679, 1772, 1835, 2108, 2298
DdeI CTNAG 6 cut(s) 41, 166, 255, 260, 1804, 2235
DinI GGCGCC 4 cut(s) 577, 712, 756, 1563
DrdI GACNNNNNNGTC 5 cut(s) 425, 431, 452, 1003, 1666
DseDI GACNNNNNNGTC 5 cut(s) 425, 431, 452, 1003, 1666
EaeI YGGCCR 4 cut(s) 458, 532, 1056, 1986
EagI CGGCCG 1 cut(s) 532
EciI GGCGGA 4 cut(s) 100, 595, 952, 1735
Ecl136II GAGCTC 1 cut(s) 1188
EclXI CGGCCG 1 cut(s) 532
Eco130I CCWWGG 1 cut(s) 1010
Eco24I GRGCYC 2 cut(s) 369, 1190
Eco31I GGTCTC 1 cut(s) 521
Eco47I GGWCC 6 cut(s) 277, 402, 652, 1590, 1604, 1999
Eco52I CGGCCG 1 cut(s) 532
Eco53kI GAGCTC 1 cut(s) 1188
Eco57I CTGAAG 1 cut(s) 40
Eco88I CYCGRG 3 cut(s) 362, 1855, 1873
EcoICRI GAGCTC 1 cut(s) 1188
EcoO109I RGGNCCY 2 cut(s) 1517, 1999
EcoRI GAATTC 2 cut(s) 1165, 2157
EcoRII CCWGG 3 cut(s) 181, 654, 1899
EcoT14I CCWWGG 1 cut(s) 1010
EcoT38I GRGCYC 2 cut(s) 369, 1190
EgeI GGCGCC 4 cut(s) 577, 712, 756, 1563
EheI GGCGCC 4 cut(s) 577, 712, 756, 1563
ErhI CCWWGG 1 cut(s) 1010
Esp3I CGTCTC 3 cut(s) 186, 1275, 1491
FaeI CATG 8 cut(s) 245, 425, 1674, 1682, 1775, 1838, 2111, 2301
FaqI GGGAC 6 cut(s) 388, 665, 682, 1161, 1672, 2012
FatI CATG 8 cut(s) 241, 421, 1670, 1678, 1771, 1834, 2107, 2297
FauI CCCGC 1 cut(s) 1150
FbaI TGATCA 2 cut(s) 18, 1831
FokI GGATG 4 cut(s) 80, 139, 1324, 2273
FriOI GRGCYC 2 cut(s) 369, 1190
FspBI CTAG 6 cut(s) 284, 1032, 1266, 1467, 1710, 2220
GlaI GCGC 7 cut(s) 509, 520, 577, 712, 756, 1563, 2094
GsaI CCCAGC 1 cut(s) 793
GsuI CTGGAG 2 cut(s) 532, 1113
HaeII RGCGCY 5 cut(s) 511, 579, 714, 758, 1565
HaeIII GGCC 8 cut(s) 460, 534, 839, 979, 1058, 1519, 1988, 2088
HgaI GACGC 4 cut(s) 37, 235, 841, 1136
HhaI GCGC 7 cut(s) 510, 521, 578, 713, 757, 1564, 2095
Hin1I GRCGYC 7 cut(s) 246, 426, 576, 711, 755, 852, 1562
Hin1II CATG 8 cut(s) 245, 425, 1674, 1682, 1775, 1838, 2111, 2301
Hin6I GCGC 7 cut(s) 508, 519, 576, 711, 755, 1562, 2093
HinP1I GCGC 7 cut(s) 508, 519, 576, 711, 755, 1562, 2093
HindIII AAGCTT 1 cut(s) 1121
HphI GGTGA 6 cut(s) 121, 999, 1000, 1009, 1300, 2161
Hpy166II GTNNAC 2 cut(s) 1573, 2269
Hpy188III TCNNGA 6 cut(s) 1512, 1772, 1793, 1868, 1910, 2056
Hpy8I GTNNAC 2 cut(s) 1573, 2269
Hpy99I CGWCG 8 cut(s) 251, 437, 575, 692, 845, 857, 1028, 1612
HpyAV CCTTC 3 cut(s) 1270, 1855, 2251
HpyCH4III ACNGT 8 cut(s) 688, 737, 974, 1024, 1063, 1485, 2062, 2074
HpyCH4IV ACGT 4 cut(s) 396, 426, 706, 1284
HpyCH4V TGCA 7 cut(s) 74, 558, 1273, 1502, 1923, 1994, 2269
HpyF3I CTNAG 6 cut(s) 41, 166, 255, 260, 1804, 2235
HpySE526I ACGT 4 cut(s) 396, 426, 706, 1284
Hsp92I GRCGYC 7 cut(s) 246, 426, 576, 711, 755, 852, 1562
Hsp92II CATG 8 cut(s) 245, 425, 1674, 1682, 1775, 1838, 2111, 2301
HspAI GCGC 7 cut(s) 508, 519, 576, 711, 755, 1562, 2093
KasI GGCGCC 4 cut(s) 575, 710, 754, 1561
KroI GCCGGC 1 cut(s) 757
KroNI GCCGGC 1 cut(s) 759
Ksp22I TGATCA 2 cut(s) 18, 1831
KspI CCGCGG 2 cut(s) 506, 538
LmnI GCTCC 5 cut(s) 339, 965, 1345, 1595, 1838
Lsp1109I GCAGC 7 cut(s) 32, 263, 785, 1486, 1570, 1681, 1957
LweI GCATC 2 cut(s) 1789, 2295
MaeI CTAG 6 cut(s) 284, 1032, 1266, 1467, 1710, 2220
MaeII ACGT 4 cut(s) 396, 426, 706, 1284
MaeIII GTNAC 5 cut(s) 1005, 1976, 2074, 2102, 2200
MboII GAAGA 8 cut(s) 43, 46, 577, 640, 710, 1246, 1303, 1808
MfeI CAATTG 1 cut(s) 2286
MflI RGATCY 2 cut(s) 695, 1213
MhlI GDGCHC 3 cut(s) 369, 1190, 2271
MlsI TGGCCA 1 cut(s) 1988
MluCI AATT 6 cut(s) 1165, 1815, 2016, 2157, 2165, 2286
MluI ACGCGT 1 cut(s) 729
MluNI TGGCCA 1 cut(s) 1988
Mly113I GGCGCC 4 cut(s) 576, 711, 755, 1562
MlyI GAGTC 6 cut(s) 309, 420, 611, 664, 1000, 1735
MmeI TCCRAC 3 cut(s) 415, 441, 775
Mox20I TGGCCA 1 cut(s) 1988
MreI CGCCGGCG 1 cut(s) 757
MroNI GCCGGC 1 cut(s) 757
MscI TGGCCA 1 cut(s) 1988
MseI TTAA 1 cut(s) 1227
MslI CAYNNNNRTG 3 cut(s) 98, 488, 1683
Msp20I TGGCCA 1 cut(s) 1988
MspA1I CMGCKG 3 cut(s) 259, 505, 537
MunI CAATTG 1 cut(s) 2286
Mva1269I GAATGC 1 cut(s) 1300
MvaI CCWGG 3 cut(s) 183, 656, 1901
MvnI CGCG 5 cut(s) 505, 521, 537, 731, 2155
NaeI GCCGGC 1 cut(s) 759
NarI GGCGCC 4 cut(s) 576, 711, 755, 1562
NciI CCSGG 8 cut(s) 593, 902, 967, 986, 1566, 1657, 1874, 1875
NgoMIV GCCGGC 1 cut(s) 757
NheI GCTAGC 1 cut(s) 1031
NlaIII CATG 8 cut(s) 245, 425, 1674, 1682, 1775, 1838, 2111, 2301
NmeAIII GCCGAG 3 cut(s) 672, 702, 1759
NmuCI GTSAC 4 cut(s) 1005, 1976, 2074, 2200
PagI TCATGA 1 cut(s) 1771
PcsI WCGNNNNNNNCGW 3 cut(s) 375, 441, 849
PctI GAATGC 1 cut(s) 1300
PdiI GCCGGC 1 cut(s) 759
PfeI GAWTC 5 cut(s) 37, 122, 213, 582, 1369
Pfl23II CGTACG 2 cut(s) 703, 732
PflFI GACNNNGTC 2 cut(s) 395, 851
PfoI TCCNGGA 1 cut(s) 1655
PleI GAGTC 6 cut(s) 308, 419, 610, 664, 999, 1734
PluTI GGCGCC 4 cut(s) 579, 714, 758, 1565
PpsI GAGTC 6 cut(s) 308, 419, 610, 664, 999, 1734
Ppu21I YACGTR 1 cut(s) 707
PpuMI RGGWCCY 1 cut(s) 1999
Psp124BI GAGCTC 1 cut(s) 1190
Psp5II RGGWCCY 1 cut(s) 1999
Psp6I CCWGG 3 cut(s) 181, 654, 1899
PspFI CCCAGC 1 cut(s) 789
PspGI CCWGG 3 cut(s) 181, 654, 1899
PspLI CGTACG 2 cut(s) 703, 732
PspPI GGNCC 9 cut(s) 277, 402, 652, 977, 1517, 1590, 1604, 1999, 2087
PspPPI RGGWCCY 1 cut(s) 1999
PstNI CAGNNNCTG 1 cut(s) 2188
PsuI RGATCY 2 cut(s) 695, 1213
PsyI GACNNNGTC 2 cut(s) 395, 851
PvuII CAGCTG 1 cut(s) 259
RseI CAYNNNNRTG 3 cut(s) 98, 488, 1683
SacI GAGCTC 1 cut(s) 1190
SacII CCGCGG 2 cut(s) 506, 538
SaqAI TTAA 1 cut(s) 1227
Sau96I GGNCC 9 cut(s) 277, 402, 652, 977, 1517, 1590, 1604, 1999, 2087
ScaI AGTACT 2 cut(s) 171, 441
SchI GAGTC 6 cut(s) 309, 420, 611, 664, 1000, 1735
SduI GDGCHC 3 cut(s) 369, 1190, 2271
SfaNI GCATC 2 cut(s) 1789, 2295
SfcI CTRYAG 1 cut(s) 1758
SfoI GGCGCC 4 cut(s) 577, 712, 756, 1563
Sfr303I CCGCGG 2 cut(s) 506, 538
SgrAI CRCCGGYG 1 cut(s) 757
SgrBI CCGCGG 2 cut(s) 506, 538
SinI GGWCC 6 cut(s) 277, 402, 652, 1590, 1604, 1999
SmaI CCCGGG 1 cut(s) 1875
SmiMI CAYNNNNRTG 3 cut(s) 98, 488, 1683
SmlI CTYRAG 2 cut(s) 1910, 1949
SmoI CTYRAG 2 cut(s) 1910, 1949
Sse9I AATT 6 cut(s) 1165, 1815, 2016, 2157, 2165, 2286
SspDI GGCGCC 4 cut(s) 575, 710, 754, 1561
SspMI CTAG 6 cut(s) 284, 1032, 1266, 1467, 1710, 2220
SstI GAGCTC 1 cut(s) 1190
StyI CCWWGG 1 cut(s) 1010
TaaI ACNGT 8 cut(s) 688, 737, 974, 1024, 1063, 1485, 2062, 2074
TaiI ACGT 4 cut(s) 399, 429, 709, 1287
TaqI TCGA 9 cut(s) 141, 369, 444, 843, 855, 1169, 1406, 1610, 2099
TasI AATT 6 cut(s) 1165, 1815, 2016, 2157, 2165, 2286
TatI WGTACW 3 cut(s) 169, 439, 636
TauI GCSGC 9 cut(s) 505, 508, 537, 540, 683, 764, 827, 923, 2155
TfiI GAWTC 5 cut(s) 37, 122, 213, 582, 1369
Tru1I TTAA 1 cut(s) 1227
Tru9I TTAA 1 cut(s) 1227
TscAI CASTG 3 cut(s) 21, 1066, 1096
TseFI GTSAC 4 cut(s) 1005, 1976, 2074, 2200
TseI GCWGC 7 cut(s) 45, 251, 773, 1499, 1583, 1694, 1945
Tsp45I GTSAC 4 cut(s) 1005, 1976, 2074, 2200
TspDTI ATGAA 7 cut(s) 230, 1133, 1410, 1497, 1559, 1760, 2054
TspGWI ACGGA 5 cut(s) 647, 675, 1596, 1637, 2243
TspMI CCCGGG 1 cut(s) 1873
TspRI CASTG 3 cut(s) 21, 1066, 1096
Tth111I GACNNNGTC 2 cut(s) 395, 851
VneI GTGCAC 1 cut(s) 2267
VpaK11BI GGWCC 6 cut(s) 277, 402, 652, 1590, 1604, 1999
XapI RAATTY 4 cut(s) 1165, 1815, 2157, 2165
XcmI CCANNNNNNNNNTGG 2 cut(s) 21, 1066
XmaI CCCGGG 1 cut(s) 1873
XspI CTAG 6 cut(s) 284, 1032, 1266, 1467, 1710, 2220
ZraI GACGTC 1 cut(s) 427
ZrmI AGTACT 2 cut(s) 171, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.