Rh1AG404100

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0013557
Physical Location & Seq
Forward (+)
63145555 .. 63149021
3467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG404100.1

Sequence Viewer

Length: 2331 bp
ATGAAAATGGTTCATGTTCAAAAACCAGTGATCAAATGGCTGGTTCTGATTCTAACAAGCTCTGTCTTCTTCAGCATGGCCCTCTCTGCAAAAACCCAAACTTATATCATCCAAATGGACAAGTCCGCCAAGCCTGAATCCTTCACCACCCACCTCGATTGGTACTCATCCAAAGTAAACTCAGTACTCAACAAACCAGAAAAAGGAGACGATCAAAGCCAAGACAGAATCATTTACACTTACCAAACCGCCTTTCATGGCGTCGCTGCTCAACTGAGCAAACAAGAAGCAGACCTACTAGAGCAACAAGACGGAGTCTTGGCTATATTCCCAGAACAGAAGTACCAGCTCCACACCACCAGAAGTCCTCTGTTTCTCGGGCTCGAACCACACGACAGCACCACCGACAACGTCTGGTCCCAGCGAGTCTCCGACCACGACGTCATCGTAGGAGTACTCGACACGGGAGTCTGGCCGGAGAGCCAGAGCTTCAACGACACCGGAATGTCTCCGGTTCGGCGGCGCTGGCGCGGCGCATGCGAGACCGGCCGCGGCTTCACAAAAAGCAACTGCAACAGGAAAATCGTCGGCGCCAGAATCTTCCACCGGGGGTACGAGTCCGCCACCGGAAAAATCAACGAGAAGACGGAGTACAAGTCGCCGAGGGACCAGGACGGCCACGGGACCCACACCGCCGCAACCGTCGCCGGATCTCCTGTACGTGGCGCCAATCTTCTCGGCTACGCGTACGGTACGGCGAGAGGAATGGCGCCGGCGGCGAGGATCGCGGCGTACAAGGTGTGCTGGGTGGGAGGTTGTTTCAGCTCTGATATAATGGCCGCGGTTGATAAGGCCGTCGAGGACGGCGTCGATGTTTTGTCTATTTCTCTTGGCGGTGGAGTTTCCTCGTATTACCGGGATAGCTTGTCTATTGCGGCGTTTGGTGCGATGGAGATGGGTGTTTTTGTCTCTTGCTCCGCCGGGAACGGTGGGCCAGACCCGGTGAGTCTGACTAATGTGTCACCTTGGATCACCACCGTCGGTGCTAGCACTATGGATAGGGATTTTCCGGCCACTGTGAAGTTGGGGAATGGGAGGACTATTACTGGAGTTTCGCTTTATAGAGGGAGAATGAAGCTTGACGCAAAGAAGCTGTACCCTGTTGTGTTAGCGGGTGGGAATTCGAGTAGCCCCAATCCTAGCTCGTTGTGTTTAGAGGGTACTTTAGATCCGAAGAAAGTTAAGGGGAAGATTGTGATTTGTGATCGTGGGATTAGTCCTAGAGTGCAGAAGGGAGACGTGGTGAAGAATGCCGGAGGAGTTGGGATGATTTTGGCTAATACGGCTTCGAATGGGGAGGAGCTGGTTGCTGATTGTCATCTGATTCCGGCGGTTGCGGTGGGAGAGAATGAAGCAAAATCGATCAAGCATTACGCCTTAACAAGTCCGAAAGCAAAGGCAACTCTAGCATTTGTTGGTACTAGACTAGGGGTTAGACCGTCTCCCATTGTGGCTGCATTTTCGTCAAGAGGGCCTAATCTTTTGAGTTTTGAGATTATGAAGCCGGATTTGGTGGCGCCGGGAGTGAACATTCTTGCTGCTTGGACTGGGGCATTGGGTCCGTCGAGCTTGGCAACGGATCGCAGGAGAGTGAAGTTCAATATCTTGTCCGGGACTTCAATGTCGTGTCCTCATGTGAGTGGGATTGCTGCTTTGCTCAAATCTAGGCACCCCGAATGGAGTCCAGCCGCGATCAAATCCGCCTTGATGACTACAGCTTATGTTCATGACAACACACATAAGCCTCTGGAAGATGCCTCAACAGCTAAAATTTCAACCCCTTTTGATCATGGAGCTGGACACATAAACCCGAGGAAGGCTCTTGACCCGGGCTTGGTTTATGACATTCAGCCCCAGGATTATCTTGAGTTCCTCTGCACACAAAAACTAACCCCAACGCAGCTCAAGGTGTTTAGCAATAGCACTTGCCGTCACACTCTGGCCAATGCAGGGGACCTCAACTATCCGGCAATTTCCGTTGTGTTTGTGGAGAACACTAATGTCTCTGTCTTGACAGTTCATAGAACTGTCACTAATGTTGGCCCTGCGGTTTCGAGTTACCATGCCATAGTTTCGCCATTCAAGGGTGCTTATGTGAAAGTTGAGCCGCGAATTCTGAAGTTCACCAAAGCTAATCAGAAGCTGTCTTACAAAGTGACCTTTACTACAAAGACTAGGCAGACGGAACCTGAGTTTGGAGGCTTGGTGTGGAAGGCTGGAGTCCACAGAGTTAGAAGTCCAATTGTTGTGGCATGGGGGATGCTGATATGA

Protein Analysis

776

Amino Acids

83.63

Weight (kDa)

9.36

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 118 2e-20 Peptidase inhibitor I9
Peptidase_S8 PF00082 146 - 599 6.7e-51 Subtilase family
PA PF02225 386 - 475 1.7e-13 PA domain
fn3_6 PF17766 672 - 770 1e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013557)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51750
fragaria_vesca FvH4_7g28501
malus_domestica MD01G1188800.v1.1 MD01G1189100.v1.1 MD01G1189900.v1.1 MD01G1190400.v1.1
prunus_persica Prupe.2G285500_v2.0.a1
pyrus_communis pycom01g20050
rosa_chinensis RchiOBHm_Chr1g0375891
rosa_laevigata RLG00000026646
rosa_multiflora Rmu_sc0001943.1_g000010
rosa_roxburghii Rroxscaffold_4G00282550
rosa_rugosa Rorug01G0392200
rosa_samantha Rh1AG404100 Rh1BG367500 Rh1CG380300 Rh1DG397500
rosa_wichuraiana Rw0G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 440, 467, 1018, 1681
AatII GACGTC 1 cut(s) 444
AccB1I GGYRCC 5 cut(s) 590, 725, 769, 1576, 1728
AccII CGCG 7 cut(s) 531, 552, 746, 788, 842, 1751, 2170
AclWI GGATC 5 cut(s) 718, 791, 1037, 1223, 1647
AcoI YGGCCR 6 cut(s) 473, 547, 676, 837, 1071, 2001
AcsI RAATTY 3 cut(s) 1180, 1830, 2172
AcuI CTGAAG 2 cut(s) 55, 2198
AcyI GRCGYC 7 cut(s) 261, 441, 591, 726, 770, 867, 1577
AfiI CCNNNNNNNGG 7 cut(s) 203, 722, 1876, 1894, 2010, 2144, 2255
AflIII ACRYGT 1 cut(s) 744
AgsI TTSAA 6 cut(s) 20, 493, 1660, 1680, 1836, 2143
AjiI CACGTC 1 cut(s) 1300
AjnI CCWGG 2 cut(s) 669, 1914
AjuI GAANNNNNNNTTGG 6 cut(s) 237, 269, 1553, 1585, 2238, 2270
Alw26I GTCTC 8 cut(s) 201, 433, 513, 536, 973, 1290, 1506, 2068
AlwI GGATC 5 cut(s) 718, 791, 1037, 1223, 1647
AlwNI CAGNNNCTG 1 cut(s) 2203
Ama87I CYCGRG 3 cut(s) 377, 1870, 1888
ApeKI GCWGC 5 cut(s) 266, 1514, 1598, 1709, 1960
ApoI RAATTY 3 cut(s) 1180, 1830, 2172
AspLEI GCGC 7 cut(s) 525, 531, 536, 593, 728, 772, 1579
AspS9I GGNCC 9 cut(s) 79, 417, 667, 684, 992, 1532, 1619, 2014, 2102
AsuC2I CCSGG 8 cut(s) 608, 917, 982, 1001, 1581, 1672, 1889, 1890
AsuHPI GGTGA 6 cut(s) 136, 1014, 1015, 1024, 1315, 2176
AsuII TTCGAA 1 cut(s) 1349
AsuNHI GCTAGC 1 cut(s) 1046
AvaI CYCGRG 3 cut(s) 377, 1870, 1888
AvaII GGWCC 5 cut(s) 417, 667, 684, 1619, 2014
BaeI ACNNNNGTAYC 2 cut(s) 326, 359
BalI TGGCCA 1 cut(s) 2003
BanI GGYRCC 5 cut(s) 590, 725, 769, 1576, 1728
BanII GRGCYC 1 cut(s) 384
BarI GAAGNNNNNNTAC 6 cut(s) 279, 311, 635, 667, 2192, 2224
BbsI GAAGAC 2 cut(s) 58, 650
BbvI GCAGC 5 cut(s) 253, 1501, 1585, 1696, 1972
BccI CCATC 2 cut(s) 943, 949
BceAI ACGGC 6 cut(s) 691, 771, 839, 880, 1359, 1974
BciT130I CCWGG 2 cut(s) 671, 1916
BclI TGATCA 2 cut(s) 30, 1846
BcnI CCSGG 8 cut(s) 608, 917, 982, 1001, 1581, 1672, 1889, 1890
BcoDI GTCTC 8 cut(s) 201, 433, 513, 536, 973, 1290, 1506, 2068
BfaI CTAG 9 cut(s) 299, 1047, 1200, 1281, 1466, 1482, 1487, 1725, 2235
BfmI CTRYAG 1 cut(s) 1773
BfoI RGCGCY 5 cut(s) 526, 594, 729, 773, 1580
BmcAI AGTACT 2 cut(s) 186, 456
Bme18I GGWCC 5 cut(s) 417, 667, 684, 1619, 2014
BmeT110I CYCGRG 3 cut(s) 377, 1870, 1888
BmgBI CACGTC 1 cut(s) 1300
BmgT120I GGNCC 9 cut(s) 79, 417, 667, 684, 992, 1532, 1619, 2014, 2102
BmrI ACTGGG 1 cut(s) 1617
BmsI GCATC 2 cut(s) 1804, 2310
BmtI GCTAGC 1 cut(s) 1050
BmuI ACTGGG 1 cut(s) 1617
BpiI GAAGAC 2 cut(s) 58, 650
BplI GAGNNNNNCTC 2 cut(s) 1864, 1896
BpmI CTGGAG 2 cut(s) 1128, 2298
Bpu14I TTCGAA 1 cut(s) 1349
BpuEI CTTGAG 2 cut(s) 1946, 1949
BpuMI CCSGG 8 cut(s) 608, 917, 982, 1001, 1581, 1672, 1889, 1890
Bsa29I ATCGAT 1 cut(s) 1421
BsaAI YACGTR 1 cut(s) 722
BsaBI GATNNNNATC 1 cut(s) 1377
BsaHI GRCGYC 7 cut(s) 261, 441, 591, 726, 770, 867, 1577
BsaI GGTCTC 1 cut(s) 536
BsaJI CCNNGG 9 cut(s) 550, 607, 662, 679, 840, 1025, 1871, 1888, 1914
BsaWI WCCGGW 3 cut(s) 500, 511, 626
BsaXI ACNNNNNCTCC 6 cut(s) 641, 671, 1287, 1317, 2250, 2280
Bsc4I CCNNNNNNNGG 7 cut(s) 203, 722, 1876, 1894, 2010, 2144, 2255
Bse118I RCCGGY 2 cut(s) 545, 772
Bse1I ACTGG 3 cut(s) 26, 1111, 1612
Bse8I GATNNNNATC 1 cut(s) 1377
BseBI CCWGG 2 cut(s) 671, 1916
BseCI ATCGAT 1 cut(s) 1421
BseDI CCNNGG 9 cut(s) 550, 607, 662, 679, 840, 1025, 1871, 1888, 1914
BseGI GGATG 4 cut(s) 108, 167, 1332, 2325
BseJI GATNNNNATC 1 cut(s) 1377
BseLI CCNNNNNNNGG 7 cut(s) 203, 722, 1876, 1894, 2010, 2144, 2255
BseMII CTCAG 3 cut(s) 195, 266, 2241
BseNI ACTGG 3 cut(s) 26, 1111, 1612
BseRI GAGGAG 2 cut(s) 1332, 1373
BseX3I CGGCCG 1 cut(s) 547
BseXI GCAGC 5 cut(s) 253, 1501, 1585, 1696, 1972
BseYI CCCAGC 2 cut(s) 420, 804
BsgI GTGCAG 2 cut(s) 1307, 1921
Bsh1236I CGCG 7 cut(s) 531, 552, 746, 788, 842, 1751, 2170
Bsh1285I CGRYCG 1 cut(s) 550
BshNI GGYRCC 5 cut(s) 590, 725, 769, 1576, 1728
BshVI ATCGAT 1 cut(s) 1421
BsiEI CGRYCG 1 cut(s) 550
BsiHKCI CYCGRG 3 cut(s) 377, 1870, 1888
BsiWI CGTACG 1 cut(s) 747
BslFI GGGAC 5 cut(s) 403, 680, 697, 1687, 2027
BslI CCNNNNNNNGG 7 cut(s) 203, 722, 1876, 1894, 2010, 2144, 2255
BsmAI GTCTC 8 cut(s) 201, 433, 513, 536, 973, 1290, 1506, 2068
BsmBI CGTCTC 3 cut(s) 201, 1290, 1506
BsmFI GGGAC 5 cut(s) 403, 680, 697, 1687, 2027
BsmI GAATGC 1 cut(s) 1315
Bso31I GGTCTC 1 cut(s) 536
BsoBI CYCGRG 3 cut(s) 377, 1870, 1888
Bsp119I TTCGAA 1 cut(s) 1349
Bsp1286I GDGCHC 1 cut(s) 384
BspCNI CTCAG 3 cut(s) 194, 267, 2242
BspDI ATCGAT 1 cut(s) 1421
BspFNI CGCG 7 cut(s) 531, 552, 746, 788, 842, 1751, 2170
BspHI TCATGA 1 cut(s) 1786
BspOI GCTAGC 1 cut(s) 1050
BspPI GGATC 5 cut(s) 718, 791, 1037, 1223, 1647
BspT104I TTCGAA 1 cut(s) 1349
BspT107I GGYRCC 5 cut(s) 590, 725, 769, 1576, 1728
BspTNI GGTCTC 1 cut(s) 536
BsrFI RCCGGY 2 cut(s) 545, 772
BsrI ACTGG 3 cut(s) 26, 1111, 1612
BssAI RCCGGY 2 cut(s) 545, 772
BssECI CCNNGG 9 cut(s) 550, 607, 662, 679, 840, 1025, 1871, 1888, 1914
BssNI GRCGYC 7 cut(s) 261, 441, 591, 726, 770, 867, 1577
BssT1I CCWWGG 1 cut(s) 1025
Bst2UI CCWGG 2 cut(s) 671, 1916
Bst4CI ACNGT 8 cut(s) 703, 752, 989, 1039, 1078, 1500, 2077, 2089
BstACI GRCGYC 7 cut(s) 261, 441, 591, 726, 770, 867, 1577
BstBAI YACGTR 1 cut(s) 722
BstBI TTCGAA 1 cut(s) 1349
BstC8I GCNNGC 4 cut(s) 527, 538, 774, 1048
BstDEI CTNAG 3 cut(s) 181, 275, 2250
BstDSI CCRYGG 3 cut(s) 550, 679, 840
BstF5I GGATG 4 cut(s) 108, 167, 1332, 2325
BstFNI CGCG 7 cut(s) 531, 552, 746, 788, 842, 1751, 2170
BstH2I RGCGCY 5 cut(s) 526, 594, 729, 773, 1580
BstHHI GCGC 7 cut(s) 525, 531, 536, 593, 728, 772, 1579
BstMAI GTCTC 8 cut(s) 201, 433, 513, 536, 973, 1290, 1506, 2068
BstMCI CGRYCG 1 cut(s) 550
BstNI CCWGG 2 cut(s) 671, 1916
BstNSI RCATGY 1 cut(s) 540
BstSFI CTRYAG 1 cut(s) 1773
BstUI CGCG 7 cut(s) 531, 552, 746, 788, 842, 1751, 2170
BstV1I GCAGC 5 cut(s) 253, 1501, 1585, 1696, 1972
BstV2I GAAGAC 2 cut(s) 58, 650
BstX2I RGATCY 2 cut(s) 710, 1228
BstYI RGATCY 2 cut(s) 710, 1228
BstZI CGGCCG 1 cut(s) 547
Bsu15I ATCGAT 1 cut(s) 1421
BsuTUI ATCGAT 1 cut(s) 1421
BtgI CCRYGG 3 cut(s) 550, 679, 840
BtgZI GCGATG 1 cut(s) 962
BtrI CACGTC 1 cut(s) 1300
BtsCI GGATG 4 cut(s) 108, 167, 1332, 2325
BtsIMutI CAGTG 2 cut(s) 33, 1074
Cac8I GCNNGC 4 cut(s) 527, 538, 774, 1048
CaiI CAGNNNCTG 1 cut(s) 2203
CciI TCATGA 1 cut(s) 1786
CfoI GCGC 7 cut(s) 525, 531, 536, 593, 728, 772, 1579
Cfr10I RCCGGY 2 cut(s) 545, 772
Cfr13I GGNCC 9 cut(s) 79, 417, 667, 684, 992, 1532, 1619, 2014, 2102
Cfr42I CCGCGG 2 cut(s) 553, 843
Cfr9I CCCGGG 1 cut(s) 1888
ClaI ATCGAT 1 cut(s) 1421
CseI GACGC 3 cut(s) 250, 856, 1151
CspCI CAANNNNNGTGG 4 cut(s) 140, 175, 2289, 2324
CviAII CATG 9 cut(s) 14, 76, 257, 537, 1694, 1787, 1850, 2123, 2313
DdeI CTNAG 3 cut(s) 181, 275, 2250
DinI GGCGCC 4 cut(s) 592, 727, 771, 1578
DrdI GACNNNNNNGTC 4 cut(s) 440, 467, 1018, 1681
DseDI GACNNNNNNGTC 4 cut(s) 440, 467, 1018, 1681
EaeI YGGCCR 6 cut(s) 473, 547, 676, 837, 1071, 2001
EagI CGGCCG 1 cut(s) 547
EciI GGCGGA 4 cut(s) 115, 610, 967, 1750
EclXI CGGCCG 1 cut(s) 547
Eco130I CCWWGG 1 cut(s) 1025
Eco24I GRGCYC 1 cut(s) 384
Eco31I GGTCTC 1 cut(s) 536
Eco47I GGWCC 5 cut(s) 417, 667, 684, 1619, 2014
Eco52I CGGCCG 1 cut(s) 547
Eco57I CTGAAG 2 cut(s) 55, 2198
Eco88I CYCGRG 3 cut(s) 377, 1870, 1888
EcoO109I RGGNCCY 3 cut(s) 684, 1532, 2014
EcoRI GAATTC 2 cut(s) 1180, 2172
EcoRII CCWGG 2 cut(s) 669, 1914
EcoT14I CCWWGG 1 cut(s) 1025
EcoT38I GRGCYC 1 cut(s) 384
EgeI GGCGCC 4 cut(s) 592, 727, 771, 1578
EheI GGCGCC 4 cut(s) 592, 727, 771, 1578
ErhI CCWWGG 1 cut(s) 1025
Esp3I CGTCTC 3 cut(s) 201, 1290, 1506
FaeI CATG 9 cut(s) 17, 79, 260, 540, 1697, 1790, 1853, 2126, 2316
FaqI GGGAC 5 cut(s) 403, 680, 697, 1687, 2027
FatI CATG 9 cut(s) 13, 75, 256, 536, 1693, 1786, 1849, 2122, 2312
FauI CCCGC 1 cut(s) 1165
FbaI TGATCA 2 cut(s) 30, 1846
FokI GGATG 3 cut(s) 95, 154, 1339
FriOI GRGCYC 1 cut(s) 384
FspBI CTAG 9 cut(s) 299, 1047, 1200, 1281, 1466, 1482, 1487, 1725, 2235
GlaI GCGC 7 cut(s) 524, 530, 535, 592, 727, 771, 1578
GsaI CCCAGC 2 cut(s) 424, 808
GsuI CTGGAG 2 cut(s) 1128, 2298
HaeII RGCGCY 5 cut(s) 526, 594, 729, 773, 1580
HgaI GACGC 3 cut(s) 250, 856, 1151
HhaI GCGC 7 cut(s) 525, 531, 536, 593, 728, 772, 1579
Hin1I GRCGYC 7 cut(s) 261, 441, 591, 726, 770, 867, 1577
Hin1II CATG 9 cut(s) 17, 79, 260, 540, 1697, 1790, 1853, 2126, 2316
Hin6I GCGC 7 cut(s) 523, 529, 534, 591, 726, 770, 1577
HinP1I GCGC 7 cut(s) 523, 529, 534, 591, 726, 770, 1577
HindIII AAGCTT 1 cut(s) 1136
HphI GGTGA 6 cut(s) 136, 1014, 1015, 1024, 1315, 2176
Hpy166II GTNNAC 4 cut(s) 178, 1588, 2184, 2284
Hpy188I TCNGA 9 cut(s) 48, 433, 829, 1011, 1233, 1383, 1449, 2178, 2199
Hpy188III TCNNGA 6 cut(s) 1527, 1787, 1808, 1883, 1925, 2071
Hpy8I GTNNAC 4 cut(s) 178, 1588, 2184, 2284
Hpy99I CGWCG 8 cut(s) 266, 443, 590, 707, 860, 872, 1043, 1627
HpyAV CCTTC 4 cut(s) 151, 1285, 1870, 2266
HpyCH4III ACNGT 8 cut(s) 703, 752, 989, 1039, 1078, 1500, 2077, 2089
HpyCH4IV ACGT 4 cut(s) 411, 441, 721, 1299
HpyCH4V TGCA 6 cut(s) 89, 573, 1288, 1517, 1938, 2009
HpyF3I CTNAG 3 cut(s) 181, 275, 2250
HpySE526I ACGT 4 cut(s) 411, 441, 721, 1299
Hsp92I GRCGYC 7 cut(s) 261, 441, 591, 726, 770, 867, 1577
Hsp92II CATG 9 cut(s) 17, 79, 260, 540, 1697, 1790, 1853, 2126, 2316
HspAI GCGC 7 cut(s) 523, 529, 534, 591, 726, 770, 1577
KasI GGCGCC 4 cut(s) 590, 725, 769, 1576
KflI GGGWCCC 1 cut(s) 684
KroI GCCGGC 1 cut(s) 772
KroNI GCCGGC 1 cut(s) 774
Ksp22I TGATCA 2 cut(s) 30, 1846
KspI CCGCGG 2 cut(s) 553, 843
LmnI GCTCC 4 cut(s) 354, 980, 1360, 1853
Lsp1109I GCAGC 5 cut(s) 253, 1501, 1585, 1696, 1972
LweI GCATC 2 cut(s) 1804, 2310
MaeI CTAG 9 cut(s) 299, 1047, 1200, 1281, 1466, 1482, 1487, 1725, 2235
MaeII ACGT 4 cut(s) 411, 441, 721, 1299
MaeIII GTNAC 5 cut(s) 1020, 1991, 2089, 2117, 2215
MboII GAAGA 9 cut(s) 58, 61, 592, 655, 725, 1246, 1261, 1318, 1823
MfeI CAATTG 1 cut(s) 2301
MflI RGATCY 2 cut(s) 710, 1228
MhlI GDGCHC 1 cut(s) 384
MlsI TGGCCA 1 cut(s) 2003
MluCI AATT 5 cut(s) 1180, 1830, 2031, 2172, 2301
MluI ACGCGT 1 cut(s) 744
MluNI TGGCCA 1 cut(s) 2003
Mly113I GGCGCC 4 cut(s) 591, 726, 770, 1577
MlyI GAGTC 7 cut(s) 324, 435, 477, 626, 1015, 1750, 2289
MmeI TCCRAC 1 cut(s) 456
Mox20I TGGCCA 1 cut(s) 2003
MreI CGCCGGCG 1 cut(s) 772
MroNI GCCGGC 1 cut(s) 772
MscI TGGCCA 1 cut(s) 2003
MseI TTAA 2 cut(s) 1242, 1439
MslI CAYNNNNRTG 3 cut(s) 113, 503, 1698
Msp20I TGGCCA 1 cut(s) 2003
MspA1I CMGCKG 2 cut(s) 552, 842
MteI GCGCNGCGC 1 cut(s) 532
MunI CAATTG 1 cut(s) 2301
Mva1269I GAATGC 1 cut(s) 1315
MvaI CCWGG 2 cut(s) 671, 1916
MvnI CGCG 7 cut(s) 531, 552, 746, 788, 842, 1751, 2170
NaeI GCCGGC 1 cut(s) 774
NarI GGCGCC 4 cut(s) 591, 726, 770, 1577
NciI CCSGG 8 cut(s) 608, 917, 982, 1001, 1581, 1672, 1889, 1890
NgoMIV GCCGGC 1 cut(s) 772
NheI GCTAGC 1 cut(s) 1046
NlaIII CATG 9 cut(s) 17, 79, 260, 540, 1697, 1790, 1853, 2126, 2316
NmeAIII GCCGAG 2 cut(s) 687, 717
NmuCI GTSAC 4 cut(s) 1020, 1991, 2089, 2215
NspI RCATGY 1 cut(s) 540
NspV TTCGAA 1 cut(s) 1349
PaeI GCATGC 1 cut(s) 540
PagI TCATGA 1 cut(s) 1786
PcsI WCGNNNNNNNCGW 3 cut(s) 390, 444, 864
PctI GAATGC 1 cut(s) 1315
PdiI GCCGGC 1 cut(s) 774
PfeI GAWTC 5 cut(s) 49, 137, 228, 597, 1384
Pfl23II CGTACG 1 cut(s) 747
PflFI GACNNNGTC 3 cut(s) 314, 410, 866
PfoI TCCNGGA 1 cut(s) 1670
PleI GAGTC 7 cut(s) 323, 434, 476, 625, 1014, 1749, 2288
PluTI GGCGCC 4 cut(s) 594, 729, 773, 1580
PpsI GAGTC 7 cut(s) 323, 434, 476, 625, 1014, 1749, 2288
Ppu21I YACGTR 1 cut(s) 722
PpuMI RGGWCCY 2 cut(s) 684, 2014
Psp5II RGGWCCY 2 cut(s) 684, 2014
Psp6I CCWGG 2 cut(s) 669, 1914
PspFI CCCAGC 2 cut(s) 420, 804
PspGI CCWGG 2 cut(s) 669, 1914
PspLI CGTACG 1 cut(s) 747
PspPI GGNCC 9 cut(s) 79, 417, 667, 684, 992, 1532, 1619, 2014, 2102
PspPPI RGGWCCY 2 cut(s) 684, 2014
PstNI CAGNNNCTG 1 cut(s) 2203
PsuI RGATCY 2 cut(s) 710, 1228
PsyI GACNNNGTC 3 cut(s) 314, 410, 866
RseI CAYNNNNRTG 3 cut(s) 113, 503, 1698
SacII CCGCGG 2 cut(s) 553, 843
SaqAI TTAA 2 cut(s) 1242, 1439
Sau96I GGNCC 9 cut(s) 79, 417, 667, 684, 992, 1532, 1619, 2014, 2102
ScaI AGTACT 2 cut(s) 186, 456
SchI GAGTC 7 cut(s) 324, 435, 477, 626, 1015, 1750, 2289
SduI GDGCHC 1 cut(s) 384
SfaNI GCATC 2 cut(s) 1804, 2310
SfcI CTRYAG 1 cut(s) 1773
SfoI GGCGCC 4 cut(s) 592, 727, 771, 1578
Sfr303I CCGCGG 2 cut(s) 553, 843
SfuI TTCGAA 1 cut(s) 1349
SgrAI CRCCGGYG 1 cut(s) 772
SgrBI CCGCGG 2 cut(s) 553, 843
SinI GGWCC 5 cut(s) 417, 667, 684, 1619, 2014
SmaI CCCGGG 1 cut(s) 1890
SmiMI CAYNNNNRTG 3 cut(s) 113, 503, 1698
SmlI CTYRAG 2 cut(s) 1925, 1964
SmoI CTYRAG 2 cut(s) 1925, 1964
SphI GCATGC 1 cut(s) 540
Sse9I AATT 5 cut(s) 1180, 1830, 2031, 2172, 2301
SspDI GGCGCC 4 cut(s) 590, 725, 769, 1576
SspMI CTAG 9 cut(s) 299, 1047, 1200, 1281, 1466, 1482, 1487, 1725, 2235
StyI CCWWGG 1 cut(s) 1025
TaaI ACNGT 8 cut(s) 703, 752, 989, 1039, 1078, 1500, 2077, 2089
TaiI ACGT 4 cut(s) 414, 444, 724, 1302
TasI AATT 5 cut(s) 1180, 1830, 2031, 2172, 2301
TatI WGTACW 3 cut(s) 184, 454, 651
TfiI GAWTC 5 cut(s) 49, 137, 228, 597, 1384
Tru1I TTAA 2 cut(s) 1242, 1439
Tru9I TTAA 2 cut(s) 1242, 1439
TscAI CASTG 2 cut(s) 33, 1081
TseFI GTSAC 4 cut(s) 1020, 1991, 2089, 2215
TseI GCWGC 5 cut(s) 266, 1514, 1598, 1709, 1960
Tsp45I GTSAC 4 cut(s) 1020, 1991, 2089, 2215
TspDTI ATGAA 7 cut(s) 17, 245, 1148, 1425, 1574, 1775, 2069
TspGWI ACGGA 6 cut(s) 327, 662, 1611, 1652, 2026, 2258
TspMI CCCGGG 1 cut(s) 1888
TspRI CASTG 2 cut(s) 33, 1081
Tth111I GACNNNGTC 3 cut(s) 314, 410, 866
VpaK11BI GGWCC 5 cut(s) 417, 667, 684, 1619, 2014
XapI RAATTY 3 cut(s) 1180, 1830, 2172
XceI RCATGY 1 cut(s) 540
XcmI CCANNNNNNNNNTGG 2 cut(s) 33, 1081
XmaI CCCGGG 1 cut(s) 1888
XspI CTAG 9 cut(s) 299, 1047, 1200, 1281, 1466, 1482, 1487, 1725, 2235
ZraI GACGTC 1 cut(s) 442
ZrmI AGTACT 2 cut(s) 186, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.